pSB1A1 1 pSB1A1 pUC19 with a BioBrick cloning site (Replaced by pSB1A3) 2006-10-23T11:00:00Z 2015-05-08T01:14:55Z pUC19 is an extremely common cloning vector. This particular version is entered in the registry because several parts synthesized by Codon Devices (BBa_P1002, BBa_P1003, BBa_P1004, BBa_P1005, BBa_I50040) were returned in this vector. Sequence information can be found at http://www.fermentas.com/techinfo/nucleicacids/sequences/puc19.txt This plasmid is pUC19 with a BioBricks cloning site (EcoRI-NotI-XbaI-SpeI-NotI-PstI) located between the EcoRI and BamHI sites in the multiple cloning site of pUC19. false false _41_ 0 126 70 Not in stock false It is not entirely clear whether there are two contigous copies of EcoRI restriction site or one copy of the EcoRI in this vector. Sequence information did not include this section of the vector. In this part, only a single EcoRI restriction site is shown. false Reshma Shetty annotation1904908 1 RNAI transcript promoter -35 site range1904908 1 878 883 annotation1904919 1 M13 Forward priming site range1904919 1 2670 2685 annotation1904907 1 RNAI transcript range1904907 1 914 1021 annotation1904909 1 RNAI transcript promoter -35 site range1904909 1 900 906 annotation1904917 1 P(BLA) -35 site range1904917 1 2156 2161 annotation1904912 1 pBR322 range1904912 1 290 2291 annotation1904915 1 bla RNA transcription start range1904915 1 2126 2126 annotation1904911 1 RNAII transcript promoter -35 site range1904911 1 1055 1060 annotation1904916 1 P(BLA) -10 site range1904916 1 2135 2140 annotation1904905 1 RNAII transcript range1904905 1 472 1024 annotation1904913 1 beta-lactamase (bla; amp-r) range1904913 1 1231 2091 annotation1904920 1 BioBricks suffix range1904920 1 1 21 annotation1904906 1 origin of replication range1904906 1 472 1060 annotation1904914 1 beta-lactamase signal peptide range1904914 1 2023 2091 annotation1904918 1 pBR322 range1904918 1 2292 2428 annotation1904904 1 ORI range1904904 1 472 472 annotation1904910 1 RNAII transcript promoter -10 site range1904910 1 1034 1039 annotation1904901 1 P(LAC) -35 site range1904901 1 143 148 annotation1904902 1 CAP protein binding site range1904902 1 168 180 annotation1904898 1 M13Reverse priming site range1904898 1 70 86 annotation1904900 1 P(LAC) -10 site range1904900 1 119 124 annotation1904903 1 Lac operon range1904903 1 60 289 annotation1904921 1 BioBricks prefix range1904921 1 2687 2708 annotation1904899 1 P(LAC) transcription start site range1904899 1 112 112 pSB1A1_sequence 1 tactagtagcggccgctgcagggatcctctagagtcgacctgcaggcatgcaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z