##gff-version 3
##sequence-region pSB1A1 1 2708
pSB1A1	.	CDS	1231	2091	.	+	0	ID=annotation1904913;Name=beta-lactamase (bla; amp-r)
pSB1A1	.	operator	168	180	.	+	0	ID=annotation1904902;Name=CAP protein binding site
pSB1A1	.	primer_binding_site	2670	2685	.	+	0	ID=annotation1904919;Name=M13 Forward priming site
pSB1A1	.	promoter	143	148	.	+	0	ID=annotation1904901;Name=P(LAC) -35 site
pSB1A1	.	sequence_feature	112	112	.	+	0	ID=annotation1904899;Name=P(LAC) transcription start site
pSB1A1	.	sequence_feature	2687	2708	.	+	0	ID=annotation1904921;Name=BioBricks prefix
pSB1A1	.	promoter	1055	1060	.	+	0	ID=annotation1904911;Name=RNAII transcript promoter -35 site
pSB1A1	.	sequence_feature	472	1060	.	+	0	ID=annotation1904906;Name=origin of replication
pSB1A1	.	sequence_feature	472	1024	.	+	0	ID=annotation1904905;Name=RNAII transcript
pSB1A1	.	CDS	2023	2091	.	+	0	ID=annotation1904914;Name=beta-lactamase signal peptide
pSB1A1	.	promoter	2156	2161	.	+	0	ID=annotation1904917;Name=P(BLA) -35 site
pSB1A1	.	sequence_feature	914	1021	.	+	0	ID=annotation1904907;Name=RNAI transcript
pSB1A1	.	promoter	119	124	.	+	0	ID=annotation1904900;Name=P(LAC) -10 site
pSB1A1	.	sequence_feature	2292	2428	.	+	0	ID=annotation1904918;Name=pBR322
pSB1A1	.	sequence_feature	472	472	.	+	0	ID=annotation1904904;Name=ORI
pSB1A1	.	sequence_feature	1	21	.	+	0	ID=annotation1904920;Name=BioBricks suffix
pSB1A1	.	sequence_feature	60	289	.	+	0	ID=annotation1904903;Name=Lac operon
pSB1A1	.	promoter	878	883	.	+	0	ID=annotation1904908;Name=RNAI transcript promoter -35 site
pSB1A1	.	sequence_feature	290	2291	.	+	0	ID=annotation1904912;Name=pBR322
pSB1A1	.	promoter	1034	1039	.	+	0	ID=annotation1904910;Name=RNAII transcript promoter -10 site
pSB1A1	.	primer_binding_site	70	86	.	+	0	ID=annotation1904898;Name=M13Reverse priming site
pSB1A1	.	sequence_feature	2126	2126	.	+	0	ID=annotation1904915;Name=bla RNA transcription start
pSB1A1	.	promoter	2135	2140	.	+	0	ID=annotation1904916;Name=P(BLA) -10 site
pSB1A1	.	promoter	900	906	.	+	0	ID=annotation1904909;Name=RNAI transcript promoter -35 site
>pSB1A1
tactagtagcggccgctgcagggatcctctagagtcgacctgcaggcatgcaagcttggcgtaatcatggtcatagctg
tttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtg
cctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagct
gcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcg
ctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggg
ataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttttt
ccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataa
agataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccg
cctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctc
caagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaac
ccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgcta
cagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagt
taccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag
cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacg
aaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaag
ttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctca
gcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccat
ctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccgg
aagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagta
agtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggta
tggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctc
cttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattct
cttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgc
ggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcat
tggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgca
cccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaa
agggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggtta
ttgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaa
gtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtc
tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcgga
tgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatca
gagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggc
gccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaa
agggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagt
gaattcgcggccgcttctagag