LOCUS pSB1A10 5263 bp DNA linear 24-Nov-2024 DEFINITION Screening Plasmid v1.0 ACCESSION pSB1A10 VERSION pSB1A10.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=suffix misc_feature 22..37 /label=spacer artifact from 13450 misc_feature 38..58 /label=RNAse E site stem_loop 59..93 /label=Hairpin RBS 102..113 /label=B0034 CDS 120..800 /label=E1010 (mRFP1) misc_feature 801..825 /label=barcode misc_feature 834..962 /label=B0015 (Term) misc_feature 963..1044 /label=Scar from BB suffix removal by I13450 misc_feature 1045..3157 /label=psb1a3 misc_feature 3158..3238 /label=Scar from BB prefix removal by I13450 misc_feature 3239..4448 /label=I0500 (pBAD/araC) RBS 4457..4468 /label=B0034 CDS 4475..5194 /label=E0040 (EGFP) misc_feature 5203..5223 /label=RNAse E site misc_feature 5224..5241 /label=spacer artifact from 13450 misc_feature 5242..5263 /label=prefix ORIGIN 1 tactagtagc ggccgctgca gcgatccatt gcagtggtat tatttgtatt gatctcctta 61 ctatctctcg agagattagt acctttggag atctactaga gaaagaggag aaatactaga 121 tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt 181 ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta 241 cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc 301 tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg 361 actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag 421 acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca 481 aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca 541 tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa 601 tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct 661 acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca 721 tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc 781 actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca 841 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 901 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 961 tatactagag tcaattgagg tagaggtaca cacgcgaact ccgatagcca attcagagta 1021 ataaactgtg ataatcaatg cagtccggca aaaaaacggg caaggtgtca ccaccctgcc 1081 ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact 1141 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 1201 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 1261 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 1321 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 1381 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 1441 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 1501 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 1561 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 1621 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 1681 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 1741 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 1801 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 1861 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 1921 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 1981 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 2041 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 2101 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 2161 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 2221 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 2281 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 2341 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 2401 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 2461 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 2521 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 2581 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 2641 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 2701 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 2761 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 2821 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 2881 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 2941 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 3001 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 3061 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat 3121 aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttgaggtaga ggtacacacg 3181 cgaactccga tagccaattc agagtaataa actgtgataa tcaatgcatg tactagagtt 3241 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg 3301 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa 3361 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc 3421 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat 3481 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc 3541 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg 3601 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta 3661 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca 3721 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata 3781 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt 3841 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca 3901 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac 3961 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat 4021 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt 4081 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca 4141 gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc 4201 tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga 4261 ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca 4321 ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag 4381 cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt 4441 gggctagcta ctagagaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac 4501 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt 4561 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac 4621 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca 4681 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc 4741 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg 4801 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga 4861 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa 4921 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca 4981 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg 5041 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa 5101 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat 5161 tacacatggc atggatgaac tatacaaata ataatactag agtattattt gtattgatct 5221 ggtgaattag gtcttcgaat cgaattcgcg gccgcttcta gag //