LOCUS       pSB1A7                  2431 bp    DNA     linear       22-Nov-2024
DEFINITION  Transcriptionally insulated high copy BioBrick plasmid
ACCESSION   pSB1A7
VERSION     pSB1A7.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=suffix
     primer_bind     2155..2174
                     /label=VF2
     misc_feature    24..152
                     /label=B0015 (rev)
     stem_loop       38..57
                     /label=stem_loop
     stem_loop       98..141
                     /label=stem_loop
     misc_feature    153..153
                     /label=c > g
     misc_feature    153..158
                     /label=mutated PstI
     misc_feature    159..229
                     /label=E. coli his operon terminator
     stem_loop       167..201
                     /label=stem_loop
     primer_bind     294..313
                     /label=VR
     CDS             398..1012
                     /label=rep(pMB1) (rev)
     CDS             1172..2032
                     /label=ampR (rev)
     misc_feature    2231..2234
                     /label=end of terminator
     stem_loop       2244..2263
                     /label=stem_loop
     misc_feature    2273..2278
                     /label=mutated EcoRI
     misc_feature    2278..2278
                     /label=c > g
     misc_feature    2279..2407
                     /label=B0015
     stem_loop       2290..2333
                     /label=stem_loop
     stem_loop       2374..2393
                     /label=stem_loop
     misc_feature    2411..2431
                     /label=prefix
ORIGIN
        1 actagtagcg gccgctgcag gcttataaac gcagaaaggc ccacccgaag gtgagccagt
       61 gtgactctag tagagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
      121 cgactgagcc tttcgtttta tttgatgcct gggtgcagtc cggcaaaaaa acgggcaagg
      181 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt
      241 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      301 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      361 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      421 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      481 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      541 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      601 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      661 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      721 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      781 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      841 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      901 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      961 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1021 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1081 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1141 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1201 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1261 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1321 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1381 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1441 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1501 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1561 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1621 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1681 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1741 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1801 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1861 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1921 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1981 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2041 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2101 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2161 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2221 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattgcc
     2281 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2341 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2401 gtttatagca gaattcgcgg ccgcttctag a
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