pSB1A7 1 pSB1A7 Transcriptionally insulated high copy BioBrick plasmid 2006-10-18T11:00:00Z 2015-05-08T01:14:55Z Based upon pSB1A3; Includes two copies of [BBa_B0015] assembled from DNA oligos pSB1A4 was constructed by adding double terminators ([BBa_B0015]) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. This insulator blocks read-through transcription into BioBrick parts/ devices. false false _61_ 0 1144 61 It's complicated true The double terminators were assembled from small smaller overlapping ssDNA oligos. The outer-most EcoRI and PstI sites were mutated while the inner-most BioBrick cut sites were restored. false Karmella Haynes annotation1903659 1 stem_loop range1903659 1 2290 2333 annotation1903651 1 VR range1903651 1 294 313 annotation1903647 1 c > g range1903647 1 153 153 annotation1903661 1 prefix range1903661 1 2411 2431 annotation1903643 1 VF2 range1903643 1 2155 2174 annotation1903645 1 stem_loop range1903645 1 38 57 annotation1903660 1 stem_loop range1903660 1 2374 2393 annotation1903652 1 rep(pMB1) (rev) range1903652 1 398 1012 annotation1903657 1 c > g range1903657 1 2278 2278 annotation1903649 1 E. coli his operon terminator range1903649 1 159 229 annotation1903648 1 mutated PstI range1903648 1 153 158 annotation1903658 1 B0015 range1903658 1 2279 2407 annotation1903655 1 stem_loop range1903655 1 2244 2263 annotation1903654 1 end of terminator range1903654 1 2231 2234 annotation1903650 1 stem_loop range1903650 1 167 201 annotation1903653 1 ampR (rev) range1903653 1 1172 2032 annotation1903656 1 mutated EcoRI range1903656 1 2273 2278 annotation1903646 1 stem_loop range1903646 1 98 141 annotation1903644 1 B0015 (rev) range1903644 1 24 152 annotation1903642 1 suffix range1903642 1 1 20 pSB1A7_sequence 1 actagtagcggccgctgcaggcttataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgggtgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcagaattcgcggccgcttctaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z