LOCUS pSB1AC3 3055 bp DNA linear 22-Nov-2024 DEFINITION High copy BioBrick assembly plasmid ACCESSION pSB1AC3 VERSION pSB1AC3.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 3034..3055 /label=Prefix CDS 261..875 /label=rep (pMB1) (rev) misc_feature 276..276 /label=ORI CDS 1163..1821 /label=Cm R (rev) CDS 1933..2793 /label=APr (rev) stem_loop 30..64 /label=terminator stem loop stem_loop 3005..3024 /label=terminator stem loop primer_bind 2916..2935 /label=VF2 primer_bind 157..176 /label=VR stem_loop 1042..1147 /label=T0 terminator misc_feature 1237..1237 /label=Silent g->a mutation found during sequencing misc_feature 34..35 /label=deletion of two bases misc_feature 22..93 /label=E. coli his operon terminator misc_feature 2992..2995 /label=end of terminator ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1021 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg 1081 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 1141 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa 1201 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc 1261 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc 1321 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt 1381 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc 1441 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc 1501 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac 1561 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt 1621 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac 1681 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac 1741 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata 1801 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa 1861 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc 1921 gatcaactcg agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 1981 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 2041 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 2101 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 2161 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 2221 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 2281 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 2341 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 2401 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 2461 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 2521 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 2581 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 2641 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 2701 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 2761 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 2821 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 2881 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 2941 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa 3001 aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc tagag //