LOCUS pSB1AC3_1 3730 bp DNA linear 26-Dec-2024 DEFINITION pSB1AC3 with ccdB insert ACCESSION pSB1AC3_1 VERSION pSB1AC3_1.1 FEATURES Location/Qualifiers CDS 1861..2519 /label=Cm R (rev) CDS 2631..3491 /label=APr (rev) CDS 52..357 /label=ccdB (rev) CDS 359..579 /label=ccdA- (rev) misc_feature 3..18 /label=BB-Pre misc_feature 701..719 /label=BB-Post misc_feature 959..1573 /label=rep (pMB1) (rev) stem_loop 3703..3722 /label=terminator stem_loop 728..762 /label=terminator misc_feature 23..699 /label=ccD Operon (rev) primer_bind 3534..3553 /label=VF primer_bind 855..874 /label=VR misc_feature 974..974 /label=ORI primer_bind 3614..3633 /label=VF2 ORIGIN 1 ggaattcgcg gccgcatcta gagactggct gtgtataagg gagcctgaca tttatattcc 61 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac 121 ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct 181 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg 241 tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac 301 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc 361 accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag 421 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct 481 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata 541 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata 601 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca 661 tactgttatc tggcttttag taagccggat ccacgcgtta ctagtagcgg ccgctgcagt 721 ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat 781 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 1021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 1081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 1141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 1201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 1261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 1321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 1381 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 1441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 1501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 1561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 1621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 1681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc 1741 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat 1801 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat 1861 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca 1921 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg 1981 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc 2041 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc 2101 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa 2161 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt 2221 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca 2281 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga 2341 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata 2401 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt 2461 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt 2521 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt 2581 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag ttaccaatgc 2641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 2701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 2761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 2821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 2881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 2941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 3001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 3061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 3121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 3181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 3241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 3301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 3361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 3421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 3481 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 3541 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 3601 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 3661 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag 3721 gatgatttct //