LOCUS pSB1AK3 3189 bp DNA linear 22-Nov-2024 DEFINITION High copy BioBrick assembly plasmid ACCESSION pSB1AK3 VERSION pSB1AK3.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 3168..3189 /label=Prefix CDS 261..875 /label=rep (pMB1) (rev) CDS 1097..1912 /label=kanR (rev) CDS 2067..2927 /label=ampR (rev) misc_feature 276..276 /label=ORI stem_loop 3139..3158 /label=terminator stem loop stem_loop 30..64 /label=terminator stem loop primer_bind 3050..3069 /label=VF2 primer_bind 157..176 /label=VR misc_feature 34..35 /label=deletion of two bases misc_feature 22..93 /label=E. coli his terminator misc_feature 3126..3129 /label=end of terminator ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1021 acttggtctg acagctcgag tcccgtcaag tcagcgtaat gctctgccag tgttacaacc 1081 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca 1141 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact 1201 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc 1261 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat 1321 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga 1381 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt 1441 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat 1501 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt 1561 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg 1621 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa 1681 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt 1741 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg 1801 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt 1861 tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc 1921 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt 1981 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt 2041 ttgctgagtt gaaggatcag ctcgagttac caatgcttaa tcagtgaggc acctatctca 2101 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 2161 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 2221 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 2281 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 2341 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 2401 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 2461 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 2521 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 2581 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 2641 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 2701 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 2761 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 2821 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 2881 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 2941 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 3001 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 3061 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag 3121 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg 3181 cttctagag //