LOCUS pSB1AK3_1 3864 bp DNA linear 22-Nov-2024 DEFINITION PSB1AK3 with ccdB insert ACCESSION pSB1AK3_1 VERSION pSB1AK3_1.1 FEATURES Location/Qualifiers CDS 1912..2727 /label=kanR (rev) CDS 2882..3742 /label=ampR (rev) CDS 169..474 /label=ccdB (rev) CDS 476..696 /label=ccdA- (rev) misc_feature 1076..1690 /label=rep (pMB1) (rev) misc_feature 120..135 /label=BB-Pre misc_feature 818..836 /label=BB-Post primer_bind 3785..3804 /label=Vf primer_bind 972..991 /label=Vr stem_loop 90..109 /label=terminator stem_loop 845..879 /label=terminator misc_feature 1091..1091 /label=ORI misc_feature 140..816 /label=ccD Operon (rev) primer_bind 1..20 /label=VF2 ORIGIN 1 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga 121 attcgcggcc gcatctagag actggctgtg tataagggag cctgacattt atattcccca 181 gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc 241 ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc 301 atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc 361 actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc 421 cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc 481 agcccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca 541 tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca 601 tggttgtgct taccagaccg gagatattga catcatatat gccttgagca actgatagct 661 gtcgctgtca actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt 721 cgggtataca tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac 781 tgttatctgg cttttagtaa gccggatcca cgcgttacta gtagcggccg ctgcagtccg 841 gcaaaaaaac gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac 901 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 961 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 1021 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 1081 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 1141 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 1201 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 1261 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 1321 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 1381 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 1441 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 1501 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 1561 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 1621 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 1681 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 1741 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 1801 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgagtcccg 1861 tcaagtcagc gtaatgctct gccagtgtta caaccaatta accaattctg attagaaaaa 1921 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt 1981 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc 2041 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt 2101 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg 2161 tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg 2221 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc 2281 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg 2341 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa 2401 tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt 2461 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac 2521 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg 2581 cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg 2641 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctggagca 2701 agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga 2761 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg 2821 agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagctcga 2881 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 2941 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 3001 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 3061 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 3121 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 3181 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 3241 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 3301 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 3361 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 3421 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 3481 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 3541 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 3601 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 3661 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 3721 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 3781 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 3841 ttccgcgcac atttccccga aaag //