LOCUS       pSB1AK8                 3426 bp    DNA     linear       22-Nov-2024
DEFINITION  Eukaryotic terminator vector (formerly BBa_J52017)
ACCESSION   pSB1AK8
VERSION     pSB1AK8.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick suffix
     misc_feature    22..259
                     /label=terminator
     CDS             498..1112
                     /label=rep (pMB1)
     CDS             1334..2149
                     /label=kanR
     CDS             2304..3164
                     /label=ampR
     misc_feature    3405..3426
                     /label=BioBrick prefix
ORIGIN
        1 tactagtagc ggccgctgca gctagagctc gctgatcagc ctcgactgtg ccttctagtt
       61 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
      121 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
      181 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
      241 ggcatgctgg ggatatgcac cggcaaaaaa acgggcaagg tgtcaccacc ctgccctttt
      301 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
      361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
      961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1141 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1261 tggtctgaca gctcgagtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat
     1321 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1381 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1441 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1501 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1561 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1621 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1681 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1741 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1801 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     1861 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1921 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1981 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     2041 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     2101 aatttaatcg cggcctggag caagacgttt cccgttgaat atggctcata acaccccttg
     2161 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     2221 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     2281 ctgagttgaa ggatcagctc gagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2341 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2401 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2461 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2521 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2581 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2641 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2701 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2761 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2821 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2881 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     2941 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3001 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3061 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3121 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     3301 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     3361 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt
     3421 ctagag
//