LOCUS pSB1AT3 3446 bp DNA linear 22-Nov-2024 DEFINITION High copy BioBrick assembly plasmid ACCESSION pSB1AT3 VERSION pSB1AT3.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 3425..3446 /label=Prefix CDS 261..875 /label=rep (pMB1) (rev) CDS 1127..2317 /label=Tet-r CDS 2324..3184 /label=APr (rev) misc_feature 276..276 /label=ORI primer_bind 157..176 /label=VR primer_bind 3307..3326 /label=VF2 stem_loop 3396..3415 /label=terminator stem loop stem_loop 30..64 /label=terminator stem loop misc_feature 34..35 /label=deletion of two bases misc_feature 22..93 /label=E. coli his operon terminator misc_feature 3383..3386 /label=end of terminator ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1021 acttggtctg acagctcgag attctcatgt ttgacagctt atcatcgata agctttaatg 1081 cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa 1141 tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat 1201 gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta 1261 tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc 1321 actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat 1381 cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt 1441 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga 1501 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt 1561 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc 1621 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca 1681 taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg 1741 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt 1801 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc 1861 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt 1921 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc 1981 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc 2041 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc 2101 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag 2161 cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac 2221 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt 2281 gcgtcgcggt gcatggagcc gggccacctc gacctaactc gagttaccaa tgcttaatca 2341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 2401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 2461 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 2521 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 2581 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 2641 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 2701 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 2761 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 2821 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2881 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2941 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 3001 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 3061 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 3121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 3181 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 3241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 3301 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 3361 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt 3421 tctggaattc gcggccgctt ctagag //