LOCUS pSB1AT3_1 4121 bp DNA linear 22-Nov-2024 DEFINITION pSB1AT3 with ccdB insert ACCESSION pSB1AT3_1 VERSION pSB1AT3_1.1 FEATURES Location/Qualifiers CDS 3139..3999 /label=APr (rev) CDS 1942..3132 /label=Tet-r CDS 169..474 /label=ccdB (rev) CDS 476..696 /label=ccdA- (rev) misc_feature 120..135 /label=BB-Pre misc_feature 818..836 /label=BB-Post misc_feature 1076..1690 /label=rep (pMB1) (rev) primer_bind 4042..4061 /label=VF2 primer_bind 972..991 /label=VR misc_feature 1091..1091 /label=ORI stem_loop 90..109 /label=terminator stem_loop 845..879 /label=terminator ORIGIN 1 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga 121 attcgcggcc gcatctagag actggctgtg tataagggag cctgacattt atattcccca 181 gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc 241 ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc 301 atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc 361 actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc 421 cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc 481 agcccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca 541 tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca 601 tggttgtgct taccagaccg gagatattga catcatatat gccttgagca actgatagct 661 gtcgctgtca actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt 721 cgggtataca tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac 781 tgttatctgg cttttagtaa gccggatcca cgcgttacta gtagcggccg ctgcagtccg 841 gcaaaaaaac gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac 901 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 961 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 1021 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 1081 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 1141 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 1201 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 1261 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 1321 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 1381 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 1441 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 1501 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 1561 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 1621 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 1681 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 1741 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 1801 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgagattct 1861 catgtttgac agcttatcat cgataagctt taatgcggta gtttatcaca gttaaattgc 1921 taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc 1981 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg 2041 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg 2101 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc 2161 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca 2221 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt 2281 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc 2341 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg 2401 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac 2461 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc 2521 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 2581 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg 2641 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg 2701 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt 2761 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg 2821 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc 2881 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag 2941 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg 3001 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta 3061 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc 3121 acctcgacct aactcgagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 3181 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 3241 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 3301 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 3361 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 3421 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 3481 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 3541 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 3601 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 3661 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 3721 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 3781 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 3841 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 3901 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 3961 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 4021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 4081 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa g //