LOCUS       pSB1K15                 2648 bp    DNA     linear       25-Nov-2024
DEFINITION  Gateway entry vector for promoters (L4R1)
ACCESSION   pSB1K15
VERSION     pSB1K15.1  
FEATURES             Location/Qualifiers
     misc_feature    2552..2648
                     /label=attL4 Recombination Site
     misc_feature    1..125
                     /label=attR1 Recombination Site
     CDS             354..1164
                     /label=Kanamycin 
     misc_feature    1284..1957
                     /label=pUC Origin
     misc_feature    2228..2255
                     /label=rrnB T2 Transcription Terminator
     misc_feature    2387..2430
                     /label=rrnB T1 Transcription Terminator
     primer_bind     2496..2512
                     /label=M13(-20) Forward Primer
     primer_bind     125..141
                     /label=M13 Reverse Primer
ORIGIN
        1 ccaagtttgt acaaaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatgcagtca
      121 ctatgaacca actacttaga tggtattagt gacctgtaga attaattcga gctctagagc
      181 tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
      241 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
      301 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      361 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
      421 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
      481 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      541 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      601 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
      661 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      721 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      781 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      841 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
      901 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
      961 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     1021 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     1081 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     1141 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     1201 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     1261 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1321 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1381 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1441 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1501 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1561 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1621 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1681 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1741 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1801 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1861 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1921 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     1981 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2041 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2101 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2161 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     2221 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     2281 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     2341 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     2401 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     2461 taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc
     2521 tcgggcccga gttaacgcta ccatggagct ccaaataatg attttatttt gactgatagt
     2581 gacctgttcg ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtatag
     2641 aaaagttg
//