LOCUS       pSB1K3                  2204 bp    DNA     linear       26-Dec-2024
DEFINITION  High copy BioBrick assembly plasmid
ACCESSION   pSB1K3
VERSION     pSB1K3.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick suffix
     stem_loop       22..93
                     /label=E. coli his operon terminator
     stem_loop       30..64
                     /label=Stem loop
     primer_bind     157..176
                     /label=Verification reverse (VR) primer binding site
     misc_feature    261..875
                     /label=rep (pMB1)
     misc_feature    276..276
                     /label=ORI
     CDS             1097..1912
                     /label=Kanamycin resistance marker
     misc_feature    1880..1880
                     /label=G->C mutation to remove XhoI site
     misc_feature    2017..2060
                     /label=repeat region
     primer_bind     2067..2086
                     /label=Verification forward (VF2) primer binding site
     stem_loop       2143..2146
                     /label=end of terminator
     stem_loop       2156..2175
                     /label=Stem loop
     misc_feature    2185..2206
                     /label=BioBrick prefix
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
       61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
      121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
      301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1021 ttggtctgac agctcgagtc ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa
     1081 ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     1141 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     1201 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     1261 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     1321 ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact
     1381 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     1441 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     1501 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     1561 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     1621 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     1681 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     1741 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     1801 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     1861 gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     1921 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     1981 gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt
     2041 gctgagttga aggatcagct cgagtgccac ctgacgtcta agaaaccatt attatcatga
     2101 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     2161 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agag
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