##gff-version 3
##sequence-region pSB1K3 1 2206
pSB1K3	.	CDS	1097	1912	.	+	0	ID=annotation2120015;Name=Kanamycin resistance marker
pSB1K3	.	sequence_feature	276	276	.	+	0	ID=annotation2120014;Name=ORI
pSB1K3	.	sequence_feature	261	875	.	+	0	ID=annotation2120013;Name=rep (pMB1)
pSB1K3	.	point_mutation	1880	1880	.	+	0	ID=annotation2120016;Name=G->C mutation to remove XhoI site
pSB1K3	.	sequence_feature	1	21	.	+	0	ID=annotation2120009;Name=BioBrick suffix
pSB1K3	.	sequence_feature	2017	2060	.	+	0	ID=annotation2120017;Name=repeat region
pSB1K3	.	stem_loop	2143	2146	.	+	0	ID=annotation2120019;Name=end of terminator
pSB1K3	.	primer_binding_site	157	176	.	+	0	ID=annotation2120012;Name=Verification reverse (VR) primer binding site
pSB1K3	.	stem_loop	30	64	.	+	0	ID=annotation2120011;Name=Stem loop
pSB1K3	.	sequence_feature	2185	2206	.	+	0	ID=annotation2120021;Name=BioBrick prefix
pSB1K3	.	stem_loop	22	93	.	+	0	ID=annotation2120010;Name=E. coli his operon terminator
pSB1K3	.	primer_binding_site	2067	2086	.	+	0	ID=annotation2120018;Name=Verification forward (VF2) primer binding site
pSB1K3	.	stem_loop	2156	2175	.	+	0	ID=annotation2120020;Name=Stem loop
>pSB1K3
tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaaga
ttacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctc
actcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaa
ggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcga
cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgct
ctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctc
acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgac
cgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactg
gtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactag
aagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaa
caaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc
ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaa
aaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtct
gacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactc
atcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaat
gaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacat
caatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccgg
tgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactc
gcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattac
aaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttc
taatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttg
atggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctt
tgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacatt
atcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgt
tgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttat
cttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaagga
tcagctcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggc
agaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag