##gff-version 3
##sequence-region pSB1T3 1 2463
pSB1T3	.	stem_loop	30	64	.	+	0	ID=annotation1975538;Name=Stem loop
pSB1T3	.	promoter	1068	1074	.	+	0	ID=annotation1975543;Name=Promoter P1
pSB1T3	.	non_covalent_binding_site	1080	1083	.	+	0	ID=annotation1975545;Name=Bind Site 2
pSB1T3	.	stem_loop	2413	2432	.	+	0	ID=annotation1975554;Name=Stem loop
pSB1T3	.	sequence_feature	261	875	.	+	0	ID=annotation1975542;Name=rep (pMB1)
pSB1T3	.	stem_loop	2400	2403	.	+	0	ID=annotation1975553;Name=end of terminator
pSB1T3	.	primer_binding_site	2324	2343	.	+	0	ID=annotation1975552;Name=Verifiction forward (VF2) primer binding site
pSB1T3	.	sequence_alteration	34	35	.	+	0	ID=annotation1975536;Name=two base pair AC deletion
pSB1T3	.	promoter	1084	1090	.	+	0	ID=annotation1975547;Name=Promoter P2
pSB1T3	.	primer_binding_site	157	176	.	+	0	ID=annotation1975540;Name=Verification reverse (VR) primer binding site
pSB1T3	.	non_covalent_binding_site	1108	1111	.	+	0	ID=annotation1975548;Name=Bind Site 4
pSB1T3	.	non_covalent_binding_site	1065	1068	.	+	0	ID=annotation1975544;Name=Bind Site 1
pSB1T3	.	CDS	1127	2317	.	+	0	ID=annotation1975550;Name=Tetracycline resistance
pSB1T3	.	non_covalent_binding_site	1094	1097	.	+	0	ID=annotation1975546;Name=Bind Site 3
pSB1T3	.	sequence_feature	2442	2463	.	+	0	ID=annotation1975555;Name=BioBrick prefix
pSB1T3	.	sequence_feature	1	21	.	+	0	ID=annotation1975537;Name=BioBrick suffix
pSB1T3	.	sequence_alteration	2175	2175	.	+	0	ID=annotation1975551;Name=C->T mutation
pSB1T3	.	non_covalent_binding_site	1121	1124	.	+	0	ID=annotation1975549;Name=Bind Site 5
pSB1T3	.	sequence_feature	276	276	.	+	0	ID=annotation1975541;Name=ORI
pSB1T3	.	stem_loop	22	93	.	+	0	ID=annotation1975539;Name=E. coli his operon terminator
>pSB1T3
tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaaga
ttacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctc
actcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaa
ggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcga
cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgct
ctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctc
acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgac
cgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactg
gtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactag
aagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaa
caaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc
ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaa
aaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtct
gacagctcgagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgt
gctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgc
ccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctct
acgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgct
tcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctc
gcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttc
aaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataa
aaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcgg
ccgcttctagag