LOCUS pSB2K3 4425 bp DNA linear 22-Nov-2024 DEFINITION Inducible copy number BioBrick plasmid ACCESSION pSB2K3 VERSION pSB2K3.1 FEATURES Location/Qualifiers CDS 3433..4248 /label=kanR misc_feature 4404..4425 /label=prefix misc_feature 1..21 /label=suffix promoter 242..290 /label=F1 Replication Origin CDS 617..1372 /label=repE (rev) promoter 1463..1679 /label=oriS Replication Origin (rev) misc_feature 2037..2211 /label=lacOP CDS 2289..3134 /label=repL promoter 3242..3393 /label=nnpp promoters primer_bind 157..176 /label=VR primer_bind 4286..4305 /label=VF2 misc_feature 34..35 /label=deletion of two bases stem_loop 30..64 /label=terminator stem loop misc_feature 22..93 /label=E. coli his operon terminator stem_loop 4375..4394 /label=terminator stem loop misc_feature 4362..4365 /label=end of terminator ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatggcg 181 cgccacgcgt acccataata cccataatag ctgtttgcca tcgcgtatgc atcgatcacg 241 tgtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc ggatcgatcc 301 ggcgcgcacc gtgggaaaaa ctccaggtag aggtacacac gcggatagcc aattcagagt 361 aataaactgt gataatcaac cctcatcaat gatgacgaac taacccccga tatcaggtca 421 catgacgaag ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa tttggcttcc 481 ttaaaaatta cagttcaaaa agtatgagaa aatccatgca ggctgaagga aacagcaaaa 541 ctgtgacaaa ttaccctcag taggtcagaa caaatgtgac gaaccaccct caaatctgtg 601 acagataacc ctcagactat cctgtcgtca tggaagtgat atcgcggaag gaaaatacga 661 tatgagtcgt ctggcggcct ttctttttct caatgtatga gaggcgcatt ggagttctgc 721 tgttgatctc attaacacag acttgcagga agcggcggcg gaagtcaggc atacgctggt 781 aactttgagg cagctggtaa cgctctatga tccagtcgat tttcagagag acgatgcctg 841 agccatccgg cttacgatac tgacacaggg attcgtataa acgcatggca tacggattgg 901 tgatttcttt tgtttcacta agccgaaact gcgtaaaccg gttctgtaac ccgataaaga 961 agggaatgag atatgggttg atatgtacac tgtaaagccc tctggatgga ctgtgcgcac 1021 gtttgataaa ccaaggaaaa gattcatagc ctttttcatc gccggcatcc tcttcagggc 1081 gataaaaaac cacttccttc cccgcgaaac tcttcaatgc ctgccgtata tccttactgg 1141 cttccgcaga ggtcaatccg aatatttcag catatttagc aacatggatc tcgcagatac 1201 cgtcatgttc ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac agatacagca 1261 tacgtttttg atcccgggag agactatatg ccgcctcagt gaggtcgttt gactggacga 1321 ttcgcgggct atttttacgt ttcttgtgat tgataaccgc tgtttccgcc atgacagatc 1381 catgtgaagt gtgacaagtt tttagattgt cacactaaat aaaaaagagt caataagcag 1441 ggataacttt gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg ttaagggcaa 1501 tttgtcacag acaggactgt catttgaggg tgatttgtca cactgaaagg gcaatttgtc 1561 acaacacctt ctgtagaacc agcatggata aaggcctaca aggcgctcta aaaaagaaga 1621 tctaaaaact ataaaaaaaa taattataaa aatatccccg tggataagtg gataacccca 1681 agggaagttt tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct 1741 tcctcgctca ctcgaaattc ccggggatag ctttatgctt gtaaaccgtt ttgtgaaaaa 1801 atttttaaaa taaaaaaggg gacctctagg gtccccaatt aattagtaat ataatctatt 1861 aaaggtcatt caaaaggtca tccaccggat caattcccct gctcgcgcag gctgggtgcc 1921 aagctctcgg gtaacatcaa ggcccgatcc ttggagccct tgccctcccg cacgatgatc 1981 gtgccgtgat cgaaatccag atccttgacc cgcagttgca aaccctcact gatccgattc 2041 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 2101 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 2161 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 2221 attacgccaa gcgcggtacc tgtcgcggca acgcgctaac agacgtagta agaaccacca 2281 gcattgtaat gctggctaaa gtcactttcc tgagctgtat aacgatgagc gattttactt 2341 tttctggcta tgaattggcc tgctttgtaa cacactccgg tctatcccgt agcgccgggc 2401 atatcctgtc gcaatgtgca aatctcgcgg caacaaccag tgaatacttc attcacaagc 2461 ctcaccgcct gatcgcggca gaaactggtt atagccaatc aaccgtcgtt cgtgcattcc 2521 gtgaagctgt aaacaaaggt atcctgtctg tagagattgt tatcggcgat caccgtgaac 2581 gtcgcgctaa cctgtaccgg tttacaccat cctttttggc cttcgcacaa caagccaaaa 2641 atgcgctgat agaaagcaaa ttaaagatct cttcagcggc aaccaaggtt aaagctgttc 2701 tcgctaagac attggcttta tttaattttt tatccacacc cccatgtcaa aatgataccc 2761 cctccccctg tcaggatgac gtggcaataa agaataagaa gtcacaagtt aaaaaaacaa 2821 aaagatcagt ttccggcggt gccggaacaa ccagcctcaa aaaattgact tcatggatcg 2881 ctaaggcaaa agcaaaggct gacaatctgc ggttatccaa aaaacgcact caaaaacatg 2941 agttcaagca gaaagtagag gcggctgcgc ggaaatatgc ttacctgaag aacaagcgtt 3001 cgcctgatat tggcgggata tcaaacttcg ataacctacc gcattgcatg acggtaaacg 3061 aagctcttaa tgcggtttta gccaaaaata aagataacga acaatggggt ataccggcag 3121 gattcagagg gtaatgaatt gctctaatta taaccatgca tactttcaac acctctagtt 3181 tgccatgagg caaactcata ggtgtcctgg taagaggaca ctgttgccaa aactggacgc 3241 cccattattg caattaataa acaactaacg gacaattcta cctaacaata agtggagttg 3301 cggccggccg cgatccgcta gcaaagccac gttgtgtctc aaaatctctg atgttacatt 3361 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat 3421 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat 3481 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 3541 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 3601 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 3661 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 3721 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca 3781 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 3841 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 3901 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 3961 caagtctgga aagaaatgca taaactgttg ccattctcac cggattcagt cgtcactcat 4021 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 4081 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 4141 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 4201 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt 4261 aattggttgt aacactggcg agctctgcca cctgacgtct aagaaaccat tattatcatg 4321 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta 4381 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagag //