##gff-version 3
##sequence-region pSB2K3 1 4425
pSB2K3	.	sequence_feature	22	93	.	+	0	ID=annotation1824015;Name=E. coli his operon terminator
pSB2K3	.	CDS	3433	4248	.	+	0	ID=annotation1023020;Name=kanR
pSB2K3	.	stem_loop	4375	4394	.	+	0	ID=annotation1824016;Name=terminator stem loop
pSB2K3	.	stem_loop	30	64	.	+	0	ID=annotation1824014;Name=terminator stem loop
pSB2K3	.	operator	2037	2211	.	+	0	ID=annotation1023026;Name=lacOP
pSB2K3	.	CDS	2289	3134	.	+	0	ID=annotation1023027;Name=repL
pSB2K3	.	engineered_region	1	21	.	+	0	ID=annotation1023022;Name=suffix
pSB2K3	.	promoter	3242	3393	.	+	0	ID=annotation1023028;Name=nnpp promoters
pSB2K3	.	promoter	242	290	.	+	0	ID=annotation1023023;Name=F1 Replication Origin
pSB2K3	.	sequence_feature	34	35	.	+	0	ID=annotation1823781;Name=deletion of two bases
pSB2K3	.	CDS	617	1372	.	+	0	ID=annotation1023024;Name=repE (rev)
pSB2K3	.	sequence_feature	4362	4365	.	+	0	ID=annotation1824017;Name=end of terminator
pSB2K3	.	primer_binding_site	157	176	.	+	0	ID=annotation1782483;Name=VR
pSB2K3	.	promoter	1463	1679	.	+	0	ID=annotation1023025;Name=oriS Replication Origin (rev)
pSB2K3	.	primer_binding_site	4286	4305	.	+	0	ID=annotation1782484;Name=VF2
pSB2K3	.	engineered_region	4404	4425	.	+	0	ID=annotation1023021;Name=prefix
>pSB2K3
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaatggcgcgccacgcgtacccataatacccataatagctgtttgccatcgcgtatgcatcgatc
acgtgtccacgttctttaatagtggactcttgttccaaactggaacaacactcggatcgatccggcgcgcaccgtggga
aaaactccaggtagaggtacacacgcggatagccaattcagagtaataaactgtgataatcaaccctcatcaatgatga
cgaactaacccccgatatcaggtcacatgacgaagggaaagagaaggaaatcaactgtgacaaactgccctcaaatttg
gcttccttaaaaattacagttcaaaaagtatgagaaaatccatgcaggctgaaggaaacagcaaaactgtgacaaatta
ccctcagtaggtcagaacaaatgtgacgaaccaccctcaaatctgtgacagataaccctcagactatcctgtcgtcatg
gaagtgatatcgcggaaggaaaatacgatatgagtcgtctggcggcctttctttttctcaatgtatgagaggcgcattg
gagttctgctgttgatctcattaacacagacttgcaggaagcggcggcggaagtcaggcatacgctggtaactttgagg
cagctggtaacgctctatgatccagtcgattttcagagagacgatgcctgagccatccggcttacgatactgacacagg
gattcgtataaacgcatggcatacggattggtgatttcttttgtttcactaagccgaaactgcgtaaaccggttctgta
acccgataaagaagggaatgagatatgggttgatatgtacactgtaaagccctctggatggactgtgcgcacgtttgat
aaaccaaggaaaagattcatagcctttttcatcgccggcatcctcttcagggcgataaaaaaccacttccttccccgcg
aaactcttcaatgcctgccgtatatccttactggcttccgcagaggtcaatccgaatatttcagcatatttagcaacat
ggatctcgcagataccgtcatgttcctgtagggtgccatcagattttctgatctggtcaacgaacagatacagcatacg
tttttgatcccgggagagactatatgccgcctcagtgaggtcgtttgactggacgattcgcgggctatttttacgtttc
ttgtgattgataaccgctgtttccgccatgacagatccatgtgaagtgtgacaagtttttagattgtcacactaaataa
aaaagagtcaataagcagggataactttgtgaaaaaacagcttcttctgagggcaatttgtcacagggttaagggcaat
ttgtcacagacaggactgtcatttgagggtgatttgtcacactgaaagggcaatttgtcacaacaccttctgtagaacc
agcatggataaaggcctacaaggcgctctaaaaaagaagatctaaaaactataaaaaaaataattataaaaatatcccc
gtggataagtggataaccccaagggaagttttttcaggcatcgtgtgtaagcagaatatataagtgctgttccctggtg
cttcctcgctcactcgaaattcccggggatagctttatgcttgtaaaccgttttgtgaaaaaatttttaaaataaaaaa
ggggacctctagggtccccaattaattagtaatataatctattaaaggtcattcaaaaggtcatccaccggatcaattc
ccctgctcgcgcaggctgggtgccaagctctcgggtaacatcaaggcccgatccttggagcccttgccctcccgcacga
tgatcgtgccgtgatcgaaatccagatccttgacccgcagttgcaaaccctcactgatccgattcattaatgcagctgg
cacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacccc
aggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagcta
tgaccatgattacgccaagcgcggtacctgtcgcggcaacgcgctaacagacgtagtaagaaccaccagcattgtaatg
ctggctaaagtcactttcctgagctgtataacgatgagcgattttactttttctggctatgaattggcctgctttgtaa
cacactccggtctatcccgtagcgccgggcatatcctgtcgcaatgtgcaaatctcgcggcaacaaccagtgaatactt
cattcacaagcctcaccgcctgatcgcggcagaaactggttatagccaatcaaccgtcgttcgtgcattccgtgaagct
gtaaacaaaggtatcctgtctgtagagattgttatcggcgatcaccgtgaacgtcgcgctaacctgtaccggtttacac
catcctttttggccttcgcacaacaagccaaaaatgcgctgatagaaagcaaattaaagatctcttcagcggcaaccaa
ggttaaagctgttctcgctaagacattggctttatttaattttttatccacacccccatgtcaaaatgataccccctcc
ccctgtcaggatgacgtggcaataaagaataagaagtcacaagttaaaaaaacaaaaagatcagtttccggcggtgccg
gaacaaccagcctcaaaaaattgacttcatggatcgctaaggcaaaagcaaaggctgacaatctgcggttatccaaaaa
acgcactcaaaaacatgagttcaagcagaaagtagaggcggctgcgcggaaatatgcttacctgaagaacaagcgttcg
cctgatattggcgggatatcaaacttcgataacctaccgcattgcatgacggtaaacgaagctcttaatgcggttttag
ccaaaaataaagataacgaacaatggggtataccggcaggattcagagggtaatgaattgctctaattataaccatgca
tactttcaacacctctagtttgccatgaggcaaactcataggtgtcctggtaagaggacactgttgccaaaactggacg
ccccattattgcaattaataaacaactaacggacaattctacctaacaataagtggagttgcggccggccgcgatccgc
tagcaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaac
tgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgatt
aaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctat
cgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatg
agatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgc
atggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatatt
gttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtat
ttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctg
gcctgttgaacaagtctggaaagaaatgcataaactgttgccattctcaccggattcagtcgtcactcatggtgatttc
tcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgat
accaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatgg
tattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattgg
ttgtaacactggcgagctctgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgta
tcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag
g