pSB2K4 1 pSB2K4 pSB2K3-derived BioBrick plasmid free of many restriction sites 2006-01-19T12:00:00Z 2015-05-08T01:14:55Z pSB2K3 Derived from pSB2K3 with many restriction enzyme sites removed by site-directed mutagenesis (~2 dozen mutations). Plasmid is useful when there is a need to cut with a variety of different restriction enzymes. Most symmetric cutters have been removed, and some non-symmetric cutters have also including AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, and SgrAI. Works in exactly same way as pSB2K3. See <bb_part>pSB2K3</bb_part> for more information. Note that the VF2 site has a single base mutation in the middle of it. However, VF2 has been used successfully as a primer for PCR with this plasmid. false false _41_6_ 0 22 41 Not in stock false false Austin Che, Leon Chan and Sri Kosuri annotation1974302 1 incC range1974302 1 394 641 annotation1974314 1 Predicted transcription start range1974314 1 1426 1426 annotation1974350 1 C->T range1974350 1 4119 4119 annotation1974320 1 Possible -10 range1974320 1 1455 1463 annotation1974325 1 repE binding site range1974325 1 1527 1537 annotation1974319 1 G->T range1974319 1 1454 1454 annotation1974345 1 T->A range1974345 1 3507 3507 annotation1974328 1 oriS range1974328 1 1490 1706 annotation1974322 1 Predicted transcription start range1974322 1 1460 1460 annotation1974354 1 Stem loop range1974354 1 4402 4421 annotation1974310 1 repE range1974310 1 644 1399 annotation1974352 1 G->C range1974352 1 4321 4321 annotation1974305 1 T->C range1974305 1 578 578 annotation1974355 1 BioBrick prefix range1974355 1 4446 4467 annotation1974324 1 repE binding site range1974324 1 1497 1515 annotation1974326 1 repE binding site range1974326 1 1552 1570 annotation1974334 1 T->G range1974334 1 2699 2699 annotation1974353 1 end of terminator range1974353 1 4389 4392 annotation1974321 1 Possible -35 range1974321 1 1455 1460 annotation1974331 1 lacOP range1974331 1 2064 2238 annotation1974291 1 BioBrick suffix range1974291 1 1 21 annotation1974346 1 T->C range1974346 1 3702 3702 annotation1974330 1 Low GC region range1974330 1 1823 1844 annotation1974296 1 Verification reverse range1974296 1 184 203 annotation1974344 1 nnpp promoter 1 (.92)' range1974344 1 3375 3420 annotation1974298 1 T->A range1974298 1 292 292 annotation1974337 1 G->A range1974337 1 3017 3017 annotation1974341 1 nnpp promoter 2 (0.96)' range1974341 1 3353 3398 annotation1974323 1 Possible -35 range1974323 1 1477 1482 annotation1974348 1 kanR range1974348 1 3460 4275 annotation1974340 1 Predicted -35 range1974340 1 3359 3364 annotation1974329 1 Low GC region range1974329 1 1636 1682 annotation1974339 1 nnpp promoter 3 (0.95)' range1974339 1 3269 3314 annotation1974343 1 Predicted transcription start range1974343 1 3394 3394 annotation1974338 1 T->C range1974338 1 3251 3251 annotation1974315 1 Possible -35 range1974315 1 1426 1431 annotation1974309 1 G->C range1974309 1 857 857 annotation1974313 1 C->T range1974313 1 1142 1142 annotation1974316 1 Possible -10 range1974316 1 1433 1441 annotation1974293 1 Stem loop range1974293 1 59 91 annotation1974292 1 E. coli his operon terminator range1974292 1 51 120 annotation1974301 1 repE binding site range1974301 1 477 495 annotation1974351 1 VF2 (not an exact match, but works) range1974351 1 4313 4332 annotation1974347 1 G->C range1974347 1 3849 3849 annotation1974312 1 T->C range1974312 1 1100 1100 annotation1974307 1 repE binding site range1974307 1 623 641 annotation1974295 1 C->A range1974295 1 146 146 annotation1974311 1 G->C range1974311 1 1091 1091 annotation1974349 1 T->G range1974349 1 3903 3903 annotation1974303 1 T->A mutation range1974303 1 551 551 annotation1974297 1 f1 ori range1974297 1 269 317 annotation1974300 1 A->G mutation range1974300 1 462 462 annotation1974308 1 A->C range1974308 1 692 692 annotation1974317 1 Possible -10 range1974317 1 1444 1452 annotation1974335 1 repL range1974335 1 2316 3161 annotation1974304 1 repE binding site range1974304 1 568 586 annotation1974306 1 repE binding site range1974306 1 597 619 annotation1974336 1 T->C range1974336 1 2774 2774 annotation1974318 1 Predicted transcription start range1974318 1 1447 1447 annotation1974327 1 repE binding site range1974327 1 1581 1591 annotation1974294 1 T->C range1974294 1 93 93 annotation1974342 1 Predicted -10 range1974342 1 3379 3387 annotation1974333 1 T->G range1974333 1 2567 2567 annotation1974332 1 Maybe A or G range1974332 1 2565 2565 annotation1974299 1 repE binding site range1974299 1 394 412 pSB2K4_sequence 1 tactagtagcggccgctgcagcaccggtggcagttcgaatgatcatgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttccttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactagctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatggcgcggctcgcgtacccataatacccataatagctgtttgccatcgcgtatgcatcgatcacgtgtccacgttctttaatagtggacacttgttccaaactggaacaacactcggatcgaaccgccgcgcaccgtgggaaaaactccaggtagaggtacacacgcggatagccaattcagagtaataaactgtgataatcaaccctcatcaatgatgacgaactaacccccgatatcaggtcacatgacgaagggaaggagaaggaaatcaactgtgacaaactgccctcaaatttggcttccttaaaaattacagttcaaaaagtatgagaaaatccatgcaggcagaaggaaacagcaaaactgtgacaaactaccctcagtaggtcagaacaaatgtgacgaaccaccctcaaatctgtgacagataaccctcagactatcctgtcgtcatggaagtgatatcgcggaaggaaaatacgatatgcgtcgtctggcggcctttctttttctcaatgtatgagaggcgcattggagttctgctgttgatctcattaacacagacttgcaggaagcggcggcggaagtcaggcatacgctggtaactttgaggcagctggtaacgctctatgatccagtcgattttcagagacacgatgcctgagccatccggcttacgatactgacacagggattcgtataaacgcatggcatacggattggtgatttcttttgtttcactaagccgaaactgcgtaaaccgattctgtaacccgataaagaagggaatgagatatgggttgatatgtacactgtaaagccctctggatggactgtgcgcacgtttgataaaccaaggaaaagattcatagcctttttcatcacccgcatcctcctcagggcgataaaaaaccacttccttccccgcgaaactctttaatgcctgccgtatatccttactggcttccgcagaggtcaatccgaatatttcagcatatttagcaacatggatctcgcagataccgtcatgttcctgtagggtgccatcagattttctgatctggtcaacgaacagatacagcatacgtttttgatcccgagagagactatatgccgcctcagtgaggtcgtttgactggacgattcgcgggctatttttacgtttcttgtgattgataaccgctgtttccgccatgacagatccatgtgaagtgtgacaagtttttagattgtcacactaaataaaaaatagtcaataagcagggataactttgtgaaaaaacagcttcttctgagggcaatttgtcacagggttaagggcaatttgtcacagacaggactgtcatttgagggtgatttgtcacactgaaagggcaatttgtcacaacaccttctgtagaaccagcatggataaaggcctacaaggcgctctaaaaaagaagatctaaaaactataaaaaaaataattataaaaatatccccgtggataagtggataaccccaagggaagttttttcaggcatcgtgtgtaagcagaatatataagtgctgttccctggtgcttcctcgctcactcgaaattgccggggatagctttatgcttgtaaaccgttttgtgaaaaaatttttaaaataaaaaaggggacctctagggtccccaattaattagtaatataatctattaaaggtcattcaaaaggtcatccaccggatcaattcccctgctcgcgcaggctgggtgccaagctctcgggtaacatcaaggcccgatccttggagcccttgccctcccgcacgatgatcgtgccgtgatcgaaatccagatccttgacccgcagttgcaaaccctcactgatccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcgcggtacctgtcgcggcaacgcgctaacagacgtagtaagaaccaccagcattgtaatgctggctaaagtcactttcctgagctgtataacgatgagcgattttactttttctggctatgaattggcctgctttgtaacacactccggtctatcccgtagcgccgggcatatcctgtcgcaatgtgcaaatctcgcggcaacaaccagtgaatacttcattcacaagcctcaccgcctgatcgcggcagaaactggttatagccaatcaaccgtcgttcgtgcattccgtgaagctgtaaacaaagggatcctgtctgtagagattgttatcggcgatcaccgtgaacgtcgcgctaacctgtatcggtttacaccatcctttttggccttcgcacaacaagccaaaaatgcgctgatagaaagcaaattaaagatctcgtcagcggcaaccaaggttaaagctgttctcgctaagacattggctttatttaattttttatccacacccccatgccaaaatgataccccctccccctgtcaggatgacgtggcaataaagaataagaagtcacaagttaaaaaaacaaaaagatcagtttccggcggtgccggaacaaccagcctcaaaaaattgacttcatggatcgctaaggcaaaagcaaaggctgacaatctgcggttatccaaaaaacgcactcaaaaacatgagttcaagcagaaagtagaggcggctgcgcggaaatatgcttacctgaaaaacaagcgttcgcctgatattggcgggatatcaaacttcgataacctaccgcattgcatgacggtaaacgaagctcttaatgcggttttagccaaaaataaagataacgaacaatggggtataccggcaggattcagagggtaatgaattgctctaattataaccatgcatactttcaacacctctagtttgccatgaggcaaactcataggtgtcctggtaagaggacactgctgccaaaactggacgccccattattgcaattaataaacaactaacggacaattctacctaacaataagtggagttgcggcccgccgcgatccgctaggaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcaaggccgcgattaaaatccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctaccgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccgcttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcggctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaactgttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagttggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcgagctgtgccacctcacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggtattctgcggaccggaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z