LOCUS pSB3C5 2738 bp DNA linear 22-Nov-2024 DEFINITION Low to medium copy BioBrick standard vector ACCESSION pSB3C5 VERSION pSB3C5.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 22..26 /label=Nt.BstNB I recognition site misc_feature 27..29 /label=Arbitrary sequence (could be any bases) misc_feature 30..34 /label=Topoisomerase I recognition site misc_feature 30..30 /label=Generated T overhang misc_feature 22..34 /label=BBa_B0044 misc_feature 36..38 /label=stop codon (+2 frame) misc_feature 35..37 /label=stop codon (-1 frame) misc_feature 40..42 /label=stop codon (+3 frame) misc_feature 44..46 /label=stop codon (+1 frame) misc_feature 43..45 /label=stop codon (-2 frame) misc_feature 39..41 /label=stop codon (-3 frame) misc_feature 35..46 /label=BBa_B0042 stem_loop 57..88 /label=stem loop misc_feature 50..89 /label=identical to E. coli MG1655 between tonB and yciA terminator 47..115 /label=BBa_B0054 primer_bind 116..135 /label=VR misc_feature 116..135 /label=BBa_G00102 stem_loop 149..168 /label=stem_loop terminator 136..176 /label=BBa_B0062 misc_feature 177..183 /label=BBa_G0003 misc_feature 582..1406 /label=p15A origin of replication misc_feature 1406..1633 /label=repeat region misc_feature 184..1737 /label=BBa_I50032 misc_feature 1738..1745 /label=BBa_G0002 CDS 1852..2514 /label=cmR misc_feature 1746..2514 /label=BBa_P1004 misc_feature 2515..2521 /label=BBa_G0004 stem_loop 2530..2564 /label=stem_loop terminator 2522..2593 /label=BBa_B0053 primer_bind 2594..2613 /label=VF2 primer 2594..2613 /label=BBa_G00100 stem_loop 2649..2670 /label=stem loop from bacteriophage T3 misc_feature 2641..2679 /label=identical to bacteriophage T3 terminator 2614..2691 /label=BBa_B0055 misc_feature 2693..2695 /label=stop codon (+2 frame) misc_feature 2692..2694 /label=stop codon (-1 frame) misc_feature 2697..2699 /label=stop codon (+3 frame) misc_feature 2701..2703 /label=stop codon (+1 frame) misc_feature 2700..2702 /label=stop codon (-2 frame) misc_feature 2696..2698 /label=stop codon (-3 frame) misc_feature 2692..2703 /label=BBa_B0042 misc_feature 2704..2708 /label=Topoisomerase I recognition site misc_feature 2712..2716 /label=Nt.BstNB I recognition site misc_feature 2709..2711 /label=Arbitrary sequence (could be any bases) misc_feature 2708..2708 /label=Generated T overhang misc_feature 2704..2716 /label=BBa_B0043 misc_feature 2717..2722 /label=EcoRI site misc_feature 2732..2737 /label=XbaI site misc_feature 2717..2738 /label=Prefix misc_feature 2723..2730 /label=NotI site misc_feature 2717..2738 /label=BBa_G00000 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gagatggaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt 601 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg 661 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag 721 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga 781 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc 841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt 901 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga 961 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg 1021 agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt 1081 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg 1141 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca 1201 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta 1261 gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg 1321 gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac 1381 atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct 1441 gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg 1501 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa 1561 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt 1621 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat 1681 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac 1741 tagaggttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta 1801 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa 1861 aaaatcacgg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag 1921 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc 1981 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt 2041 gcccgcctga tgaacgctca cccggagttt cgtatggcca tgaaagacgg tgagctggtg 2101 atctgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttcg 2161 tccctctgga gtgaatacca cgacgatttc cggcagtttc tccacatata ttcgcaagat 2221 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt 2281 tttgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg 2341 gacaacttct tcgcccccgt tttcacgatg ggcaaatatt atacgcaagg cgacaaggtg 2401 ctgatgccgc tggcgatcca ggttcatcat gccgtttgtg atggcttcca tgtcggccgc 2461 atgcttaatg aattacaaca gtactgtgat gagtggcagg gcggggcgta ataatactag 2521 ctccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag 2581 attacttcgc gtttgccacc tgacgtctaa gaaaaggaat attcagcaat ttgcccgtgc 2641 cgaagaaagg cccacccgtg aaggtgagcc agtgagttga ttgctacgta attagttagt 2701 tagcccttag tgactcgaat tcgcggccgc ttctagag //