LOCUS pSB3K1 3064 bp DNA linear 22-Nov-2024 DEFINITION pACYC177 converted into BioBrick vector (formerly BBa_I739202) ACCESSION pSB3K1 VERSION pSB3K1.1 FEATURES Location/Qualifiers misc_feature 483..1312 /label=p15A replication origin misc_feature 1637..2453 /label=kanamycin resistance marker misc_feature 22..332 /label=partial ampicillin resistance marker (nonfunctional) misc_feature 445..482 /label=Tn3 inverted repeat misc_feature 1307..1534 /label=Tn903 inverted repeat misc_feature 2515..2742 /label=Tn903 inverted repeat misc_feature 2742..3036 /label=partial Tn903 repressor fragment misc_feature 1..21 /label=BioBrick suffix misc_feature 3043..3064 /label=BioBrick prefix ORIGIN 1 tactagtagc ggccgctgca gcaatggcaa caacgttgcg caaactatta actggcgaac 61 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 121 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 181 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 241 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 301 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 361 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 421 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 481 ccttaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa 541 acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc aactctttga 601 accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca gtttagcctt 661 aaccggcgca tgacttcaag actaactcct ctaaatcaat taccagtggc tgctgccagt 721 ggtgcttttg catgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag 781 cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg 841 gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt 901 aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa acgcctggta 961 tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt 1021 gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta 1081 agtatcttcc tggcatcttc caggaaatct ccgccccgtt cgtaagccat ttccgctcgc 1141 cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tatcctgtat 1201 cacatattct gctgacgcac cggtgcagcc ttttttctcc tgccacatga agcacttcac 1261 tgacaccctc atcagtgcca acatagtaag ccagtataca ctccgctagc gctgaggtct 1321 gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca 1381 gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt 1441 gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt 1501 caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg 1561 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa 1621 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg 1681 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg 1741 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct 1801 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg 1861 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc 1921 atggttactc accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc 1981 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat 2041 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc 2101 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc 2161 tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt 2221 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg 2281 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa 2341 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga 2401 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga 2461 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcggctt 2521 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac 2581 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa 2641 gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcctgg 2701 tatgagtcag caacaccttc ttcacgaggc agacctcagc gctcaaagat gcaggggtaa 2761 aagctaaccg catctttacc gacaaggcat ccggcagttc aacagatcgg gaagggctgg 2821 atttgctgag gatgaaggtg gaggaaggtg atgtcattct ggtgaagaag ctcgaccgtc 2881 ttggccgcga caccgccgac atgatccaac tgataaaaga gtttgatgct cagggtgtag 2941 cggttcggtt tattgacgac gggatcagta ccgacggtga tatggggcaa atggtggtca 3001 ccatcctgtc ggctgtggca caggctgaac gccggaggat ccgaattcgc ggccgcttct 3061 agag //