LOCUS pSB3K3 2750 bp DNA linear 22-Dec-2024 DEFINITION Low to medium copy BioBrick standard vector ACCESSION pSB3K3 VERSION pSB3K3.1 FEATURES Location/Qualifiers misc_feature 2729..2750 /label=Prefix misc_feature 1..21 /label=Suffix CDS 239..1054 /label=kanR (rev) CDS 1386..2210 /label=p15A ORI (rev) primer_bind 2611..2630 /label=VF2 primer_bind 157..176 /label=VR misc_feature 34..35 /label=deletion of two bases misc_feature 22..93 /label=E. coli his operon terminator stem_loop 30..64 /label=terminator stem loop stem_loop 2700..2719 /label=terminator stem loop misc_feature 2687..2690 /label=end of terminator ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg 181 agtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt 241 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac 301 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata 361 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta 421 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg 481 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc 541 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg 601 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat 661 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt 721 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat 781 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta 841 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca 901 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat 961 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc 1021 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt 1081 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga 1141 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc 1201 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca 1261 ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg 1321 atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc 1381 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt 1441 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg 1501 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg 1561 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg 1621 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc 1681 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg 1741 cgtttcccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt 1801 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg 1861 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct 1921 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag 1981 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa 2041 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag 2101 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag 2161 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa ggggtctgac 2221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 2281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 2341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 2461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 2581 ttatccgcct ccatccagtc tattccatgg tgccacctga cgtctaagaa accattatta 2641 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat 2701 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag //