LOCUS pSB3K5 2936 bp DNA linear 22-Nov-2024 DEFINITION Low to medium copy BioBrick standard vector ACCESSION pSB3K5 VERSION pSB3K5.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 22..26 /label=Nt.BstNB I recognition site misc_feature 27..29 /label=Arbitrary sequence (could be any bases) misc_feature 30..34 /label=Topoisomerase I recognition site misc_feature 30..30 /label=Generated T overhang misc_feature 22..34 /label=BBa_B0044 misc_feature 36..38 /label=stop codon (+2 frame) misc_feature 35..37 /label=stop codon (-1 frame) misc_feature 40..42 /label=stop codon (+3 frame) misc_feature 44..46 /label=stop codon (+1 frame) misc_feature 43..45 /label=stop codon (-2 frame) misc_feature 39..41 /label=stop codon (-3 frame) misc_feature 35..46 /label=BBa_B0042 stem_loop 57..88 /label=stem loop misc_feature 50..89 /label=identical to E. coli MG1655 between tonB and yciA terminator 47..115 /label=BBa_B0054 primer_bind 116..135 /label=VR misc_feature 116..135 /label=BBa_G00102 stem_loop 149..168 /label=stem_loop terminator 136..176 /label=BBa_B0062 misc_feature 177..183 /label=BBa_G0003 misc_feature 582..1406 /label=p15A origin of replication misc_feature 1406..1633 /label=repeat region misc_feature 184..1737 /label=BBa_I50032 misc_feature 1738..1745 /label=BBa_G0002 CDS 1894..2712 /label=kanamycin resistance misc_feature 1746..2712 /label=BBa_P1003 misc_feature 2713..2719 /label=BBa_G0004 stem_loop 2728..2762 /label=stem_loop terminator 2720..2791 /label=BBa_B0053 primer_bind 2792..2811 /label=VF2 primer 2792..2811 /label=BBa_G00100 stem_loop 2847..2868 /label=stem loop from bacteriophage T3 misc_feature 2839..2877 /label=identical to bacteriophage T3 terminator 2812..2889 /label=BBa_B0055 misc_feature 2891..2893 /label=stop codon (+2 frame) misc_feature 2890..2892 /label=stop codon (-1 frame) misc_feature 2895..2897 /label=stop codon (+3 frame) misc_feature 2899..2901 /label=stop codon (+1 frame) misc_feature 2898..2900 /label=stop codon (-2 frame) misc_feature 2894..2896 /label=stop codon (-3 frame) misc_feature 2890..2901 /label=BBa_B0042 misc_feature 2902..2906 /label=Topoisomerase I recognition site misc_feature 2910..2914 /label=Nt.BstNB I recognition site misc_feature 2907..2909 /label=Arbitrary sequence (could be any bases) misc_feature 2906..2906 /label=Generated T overhang misc_feature 2902..2914 /label=BBa_B0043 misc_feature 2915..2920 /label=EcoRI site misc_feature 2930..2935 /label=XbaI site misc_feature 2915..2936 /label=Prefix misc_feature 2921..2928 /label=NotI site misc_feature 2915..2936 /label=BBa_G00000 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gagatggaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt 601 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg 661 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag 721 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga 781 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc 841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt 901 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga 961 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg 1021 agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt 1081 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg 1141 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca 1201 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta 1261 gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg 1321 gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac 1381 atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct 1441 gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg 1501 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa 1561 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt 1621 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat 1681 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac 1741 tagagctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca cgttgtgtct 1801 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg 1861 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct 1921 tgctcccgtc cgcgcttaaa ctccaacatg gacgctgatt tatatgggta taaatgggct 1981 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg 2041 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 2101 gtccgtctca actggctgac ggagtttatg cctctcccga ccatcaagca ttttatccgt 2161 actcctgatg atgcgtggtt actcaccacc gcgattcctg ggaaaacagc cttccaggta 2221 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggccgt gttcctgcgc 2281 cggttacatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgtgt atttcgtctt 2341 gctcaggcgc aatcacgcat gaataacggt ttggttgatg cgagtgattt tgatgacgag 2401 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc acaagctctt gccattctca 2461 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 2521 aaattaatag gttgtattga tgttggacgg gtcggaatcg cagaccgtta ccaggacctt 2581 gccattcttt ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 2641 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 2701 tttttctaat aatactagct ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt 2761 ttctttaaaa ccgaaaagat tacttcgcgt ttgccacctg acgtctaaga aaaggaatat 2821 tcagcaattt gcccgtgccg aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt 2881 gctacgtaat tagttagtta gcccttagtg actcgaattc gcggccgctt ctagag //