LOCUS pSB3T5 3252 bp DNA linear 25-Dec-2024 DEFINITION Low to medium copy BioBrick standard vector ACCESSION pSB3T5 VERSION pSB3T5.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 22..26 /label=Nt.BstNB I recognition site misc_feature 27..29 /label=Arbitrary sequence (could be any bases) misc_feature 30..34 /label=Topoisomerase I recognition site misc_feature 30..30 /label=Generated T overhang misc_feature 22..34 /label=BBa_B0044 misc_feature 36..38 /label=stop codon (+2 frame) misc_feature 35..37 /label=stop codon (-1 frame) misc_feature 40..42 /label=stop codon (+3 frame) misc_feature 44..46 /label=stop codon (+1 frame) misc_feature 43..45 /label=stop codon (-2 frame) misc_feature 39..41 /label=stop codon (-3 frame) misc_feature 35..46 /label=BBa_B0042 stem_loop 57..88 /label=stem loop misc_feature 50..89 /label=identical to E. coli MG1655 between tonB and yciA terminator 47..115 /label=BBa_B0054 primer_bind 116..135 /label=VR misc_feature 116..135 /label=BBa_G00102 stem_loop 149..168 /label=stem_loop terminator 136..176 /label=BBa_B0062 misc_feature 177..183 /label=BBa_G0003 misc_feature 582..1406 /label=p15A origin of replication misc_feature 1406..1633 /label=repeat region misc_feature 184..1737 /label=BBa_I50032 misc_feature 1738..1745 /label=BBa_G0002 promoter 1746..1834 /label=tetR promoter CDS 1835..3028 /label=tetA misc_feature 1746..3028 /label=BBa_P1005 misc_feature 3029..3035 /label=BBa_G0004 stem_loop 3044..3078 /label=stem_loop terminator 3036..3107 /label=BBa_B0053 primer_bind 3108..3127 /label=VF2 primer 3108..3127 /label=BBa_G00100 stem_loop 3163..3184 /label=stem loop from bacteriophage T3 misc_feature 3155..3193 /label=identical to bacteriophage T3 terminator 3128..3205 /label=BBa_B0055 misc_feature 3207..3209 /label=stop codon (+2 frame) misc_feature 3206..3208 /label=stop codon (-1 frame) misc_feature 3211..3213 /label=stop codon (+3 frame) misc_feature 3215..3217 /label=stop codon (+1 frame) misc_feature 3214..3216 /label=stop codon (-2 frame) misc_feature 3210..3212 /label=stop codon (-3 frame) misc_feature 3206..3217 /label=BBa_B0042 misc_feature 3218..3222 /label=Topoisomerase I recognition site misc_feature 3226..3230 /label=Nt.BstNB I recognition site misc_feature 3223..3225 /label=Arbitrary sequence (could be any bases) misc_feature 3222..3222 /label=Generated T overhang misc_feature 3218..3230 /label=BBa_B0043 misc_feature 3231..3236 /label=EcoRI site misc_feature 3246..3251 /label=XbaI site misc_feature 3231..3252 /label=Prefix misc_feature 3237..3244 /label=NotI site misc_feature 3231..3252 /label=BBa_G00000 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gagatggaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt 601 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg 661 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag 721 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga 781 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc 841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt 901 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga 961 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg 1021 agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt 1081 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg 1141 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca 1201 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta 1261 gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg 1321 gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac 1381 atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct 1441 gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg 1501 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa 1561 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt 1621 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat 1681 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac 1741 tagaggagat tctcatgttt gacagcttat catcgataag ctttaatgcg gtagtttatc 1801 acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt 1861 cattctcggc accgtcaccc tggacgctgt aggcataggc ttggttatgc cggtactgcc 1921 gggcctcttg cgggatatcg tccattccga cagtattgcc agtcactatg gcgtgctgct 1981 tgcgctctat gcgttgatgc aatttctttg cgcacccgtt ctcggagccc tgtccgaccg 2041 ctttggccgc cgtccagtcc tgctcgcttc gctccttgga gccactatcg actacgcgat 2101 catggcgacc acacccgtcc tgtggattct ctacgccgga cgcatcgtgg cgggcatcac 2161 gggtgccaca ggtgcggttg ctggtgccta tatcgccgac atcaccgatg gggaagatcg 2221 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt 2281 ggccggggga ctgttgggtg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct 2341 caacggcctc aacctcctcc tgggctgctt ccttatgcag gaatcgcata agggagagcg 2401 ccgtccgatg cccttgcgtg ccttcaatcc agtcagctcc ttccggtggg cgcggggcat 2461 gactatcgtc gccgcactta tgactgtttt ctttatcatg caactcgtag gacaggttcc 2521 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg 2581 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcacgggccc 2641 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcg ggcatggcgg ccgacgcgct 2701 gggctacgtc ttgctggcgt tcgcgacgcg cggctggatg gccttcccca ttatgattct 2761 tctcgcttcc ggcggcatcg gtatgcccgc gttgcaggcc atgctgtccc gccaagtaga 2821 tgacgaccat cagggacagc ttcaagggtc gctcgcggct cttaccagcc tcacttcgat 2881 cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt 2941 ggcatggatt gtaggtgccg ccctttacct tgtctgcctc cccgcgttgc gtcgcggtgc 3001 atggagccgg gccacctcga cctaataata ctagctccgg caaaaaaacg ggcaaggtgt 3061 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgtttgc cacctgacgt 3121 ctaagaaaag gaatattcag caatttgccc gtgccgaaga aaggcccacc cgtgaaggtg 3181 agccagtgag ttgattgcta cgtaattagt tagttagccc ttagtgactc gaattcgcgg 3241 ccgcttctag ag //