LOCUS pSB4A3 3339 bp DNA linear 03-May-2024 DEFINITION pSB4A3 (Replaced by pSB4A5) ACCESSION pSB4A3 VERSION pSB4A3.1 FEATURES Location/Qualifiers RBS 3085..3089 /label=rbs(rev) promoter 3098..3103 /label=-10 promoter 3124..3129 /label=-35 misc_feature 3276..3279 /label=end of terminator misc_feature 3318..3339 /label=prefix misc_feature 1..21 /label=suffix misc_feature 22..93 /label=E.coli His operon terminator CDS 384..1334 /label=repA (rev) RBS 1338..1350 /label=RBS (rev) promoter 1355..1374 /label=(rev) CDS 2220..3080 /label=AmpR (rev) stem_loop 2145..2174 /label=Terminator (rev) primer_bind 157..176 /label=VR primer_bind 3200..3219 /label=VF2 misc_feature 34..35 /label=deletion of two bases stem_loop 30..64 /label=terminator stem loop stem_loop 3289..3308 /label=terminator stem loop ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg 181 aggttacatt gtcgatctgt tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa 241 gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac agttttccct 301 ttgatatcta acggtgaaca gttgttctac ttttgtttgt tagtcttgat gcttcactga 361 tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat gttctctagt 421 gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcatg cttactttgc 481 atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg 541 tgtagtgttt ttcttagtcc gttacgtagg taggaatctg atgtaatggt tgttggtatt 601 ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc catttgtcta 661 tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc aaccaccaat 721 ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca ttggttaagc 781 cttttaaact catggtagtt attttcaagc attaacatga acttaaattc atcaaggcta 841 atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa ccactcataa 901 atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata ttttatgaat 961 ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc taatttttcg 1021 cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac caaaggattc 1081 ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac accataagca 1141 ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga taccgtccgt 1201 tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca taaaattagc 1261 ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca 1321 actaattcag acatacatct caattggtct aggtgatttt aatcactata ccaattgaga 1381 tgggctagtc aatgataatt acatgtcctt ttcctttgag ttgtgggtat ctgtaaattc 1441 tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa ttccgctaga 1501 cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat aaagaaagaa 1561 taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact ttagtcagtt 1621 ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa 1681 aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa tattcctttt 1741 gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt tcgctgcgct 1801 cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat ggattcatgc 1861 aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc gttttatggc 1921 gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc cctctgattt 1981 tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct aatgcaccca 2041 gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtccctagtg cttggattct 2101 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg 2161 aggtcattac tggatctatc aacaggagtc caagcgagct cgtaaacttg gtctgacagt 2221 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 2281 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 2341 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 2401 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 2461 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 2521 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 2581 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 2641 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 2701 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 2761 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 2821 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 2881 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 2941 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 3001 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 3061 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 3121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 3181 ccgcgcacat ttcccatggt gccacctgac gtctaagaaa ccattattat catgacatta 3241 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc 3301 gctaaggatg atttctggaa ttcgcggccg cttctagag //