##gff-version 3
##sequence-region pSB4A5 1 3395
pSB4A5	.	sequence_feature	3172	3178	.	+	0	ID=BBa_G0004;Name=BBa_G0004
pSB4A5	.	sequence_feature	2221	2228	.	+	0	ID=BBa_G0002;Name=BBa_G0002
pSB4A5	.	sequence_feature	3349	3360	.	+	0	ID=BBa_B0042;Name=BBa_B0042
pSB4A5	.	stop_codon	3349	3351	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
pSB4A5	.	stop_codon	3350	3352	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
pSB4A5	.	stop_codon	3354	3356	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
pSB4A5	.	stop_codon	3358	3360	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
pSB4A5	.	stop_codon	3353	3355	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
pSB4A5	.	stop_codon	3357	3359	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
pSB4A5	.	sequence_feature	184	2220	.	+	0	ID=BBa_I50042;Name=BBa_I50042
pSB4A5	.	promoter	1029	1048	.	+	0	ID=annotation2071323;Name=promoter region;Parent=BBa_I50042
pSB4A5	.	sequence_feature	920	939	.	+	0	ID=annotation2071318;Name=direct repeat;Parent=BBa_I50042
pSB4A5	.	sequence_feature	1045	1067	.	+	0	ID=annotation2071325;Name=inverted repeat;Parent=BBa_I50042
pSB4A5	.	sequence_feature	322	335	.	+	0	ID=annotation2071311;Name=inverted repeat;Parent=BBa_I50042
pSB4A5	.	sequence_feature	341	345	.	+	0	ID=annotation2071312;Name=Tn3 integration site;Parent=BBa_I50042
pSB4A5	.	sequence_feature	404	421	.	+	0	ID=annotation2071315;Name=inverted repeat;Parent=BBa_I50042
pSB4A5	.	promoter	1011	1016	.	+	0	ID=annotation2071321;Name=-35;Parent=BBa_I50042
pSB4A5	.	sequence_feature	348	364	.	+	0	ID=annotation2071313;Name=inverted repeat;Parent=BBa_I50042
pSB4A5	.	sequence_feature	2030	2044	.	+	0	ID=annotation2071331;Name=inverted repeat;Parent=BBa_I50042
pSB4A5	.	sequence_feature	320	687	.	+	0	ID=annotation2071310;Name=par region;Parent=BBa_I50042
pSB4A5	.	ribosome_entry_site	1053	1065	.	+	0	ID=annotation2071326;Name=RBS;Parent=BBa_I50042
pSB4A5	.	polyA_site	2130	2145	.	+	0	ID=annotation2071333;Name=putative polyA;Parent=BBa_I50042
pSB4A5	.	sequence_feature	818	900	.	+	0	ID=annotation2071316;Name=AT rich region;Parent=BBa_I50042
pSB4A5	.	sequence_alteration	1838	1838	.	+	0	ID=annotation2071329;Name=G to A difference with canonical pSC101 vector sequence;Parent=BBa_I50042
pSB4A5	.	sequence_alteration	1933	1933	.	+	0	ID=annotation2071330;Name=C to T difference with canonical pSC101 vector sequence;Parent=BBa_I50042
pSB4A5	.	sequence_feature	1021	1042	.	+	0	ID=annotation2071322;Name=inverted repeat;Parent=BBa_I50042
pSB4A5	.	sequence_alteration	2095	2095	.	+	0	ID=annotation2071332;Name=G to C difference with canonical pSC101 vector sequence;Parent=BBa_I50042
pSB4A5	.	sequence_alteration	2185	2185	.	+	0	ID=annotation2071335;Name=T to C difference with canonical pSC101 vector sequence;Parent=BBa_I50042
pSB4A5	.	promoter	1033	1038	.	+	0	ID=annotation2071324;Name=-10;Parent=BBa_I50042
pSB4A5	.	ribosome_entry_site	1062	1067	.	+	0	ID=annotation2071327;Name=RBS;Parent=BBa_I50042
pSB4A5	.	sequence_feature	899	918	.	+	0	ID=annotation2071317;Name=direct repeat;Parent=BBa_I50042
pSB4A5	.	sequence_feature	2146	2162	.	+	0	ID=annotation2071334;Name=inverted repeat;Parent=BBa_I50042
pSB4A5	.	sequence_feature	952	971	.	+	0	ID=annotation2071319;Name=direct repeat;Parent=BBa_I50042
pSB4A5	.	sequence_alteration	997	1002	.	+	0	ID=annotation2071320;Name=mutated SpeI site;Parent=BBa_I50042
pSB4A5	.	sequence_feature	375	398	.	+	0	ID=annotation2071314;Name=inverted repeat;Parent=BBa_I50042
pSB4A5	.	CDS	1069	2019	.	+	0	ID=annotation2071328;Name=repA;Parent=BBa_I50042
pSB4A5	.	stem_loop	229	258	.	+	0	ID=annotation2071309;Name=Terminator;Parent=BBa_I50042
pSB4A5	.	sequence_feature	35	46	.	+	0	ID=BBa_B0042;Name=BBa_B0042
pSB4A5	.	stop_codon	35	37	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
pSB4A5	.	stop_codon	36	38	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
pSB4A5	.	stop_codon	40	42	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
pSB4A5	.	stop_codon	44	46	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
pSB4A5	.	stop_codon	39	41	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
pSB4A5	.	stop_codon	43	45	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
pSB4A5	.	engineered_region	2229	3171	.	+	0	ID=BBa_P1002;Name=BBa_P1002
pSB4A5	.	ribosome_entry_site	2299	2303	.	+	0	ID=annotation1897111;Name=ampR RBS;Parent=BBa_P1002
pSB4A5	.	promoter	2259	2264	.	+	0	ID=annotation1897109;Name=ampR -35;Parent=BBa_P1002
pSB4A5	.	CDS	2308	3171	.	+	0	ID=annotation1790786;Name=ampicillin resistance;Parent=BBa_P1002
pSB4A5	.	promoter	2285	2290	.	+	0	ID=annotation1897110;Name=ampR -10;Parent=BBa_P1002
pSB4A5	.	terminator	3179	3250	.	+	0	ID=BBa_B0053;Name=BBa_B0053
pSB4A5	.	stem_loop	3187	3221	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
pSB4A5	.	sequence_feature	1	21	.	+	0	ID=BBa_G00001;Name=BBa_G00001
pSB4A5	.	sequence_feature	16	21	.	+	0	ID=annotation1934227;Name=PstI;Parent=BBa_G00001
pSB4A5	.	sequence_feature	9	16	.	+	0	ID=annotation1934228;Name=NotI;Parent=BBa_G00001
pSB4A5	.	engineered_region	1	21	.	+	0	ID=annotation1821544;Name=Suffix;Parent=BBa_G00001
pSB4A5	.	sequence_feature	2	7	.	+	0	ID=annotation1934226;Name=SpeI;Parent=BBa_G00001
pSB4A5	.	primer	3251	3270	.	+	0	ID=BBa_G00100;Name=BBa_G00100
pSB4A5	.	primer_binding_site	3251	3270	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
pSB4A5	.	terminator	47	115	.	+	0	ID=BBa_B0054;Name=BBa_B0054
pSB4A5	.	stem_loop	57	88	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
pSB4A5	.	sequence_feature	50	89	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
pSB4A5	.	sequence_feature	116	135	.	+	0	ID=BBa_G00102;Name=BBa_G00102
pSB4A5	.	primer_binding_site	116	135	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
pSB4A5	.	sequence_feature	3361	3373	.	+	0	ID=BBa_B0043;Name=BBa_B0043
pSB4A5	.	sequence_feature	3365	3365	.	+	0	ID=annotation1797104;Name=Generated T overhang;Parent=BBa_B0043
pSB4A5	.	sequence_feature	3366	3368	.	+	0	ID=annotation1797103;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0043
pSB4A5	.	sequence_feature	3369	3373	.	+	0	ID=annotation1797102;Name=Nt.BstNB I recognition site;Parent=BBa_B0043
pSB4A5	.	sequence_feature	3361	3365	.	+	0	ID=annotation1797101;Name=Topoisomerase I recognition site;Parent=BBa_B0043
pSB4A5	.	sequence_feature	22	34	.	+	0	ID=BBa_B0044;Name=BBa_B0044
pSB4A5	.	sequence_feature	27	29	.	+	0	ID=annotation1797127;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0044
pSB4A5	.	sequence_feature	30	30	.	+	0	ID=annotation1797129;Name=Generated T overhang;Parent=BBa_B0044
pSB4A5	.	sequence_feature	22	26	.	+	0	ID=annotation1797126;Name=Nt.BstNB I recognition site;Parent=BBa_B0044
pSB4A5	.	sequence_feature	30	34	.	+	0	ID=annotation1797128;Name=Topoisomerase I recognition site;Parent=BBa_B0044
pSB4A5	.	terminator	136	176	.	+	0	ID=BBa_B0062;Name=BBa_B0062
pSB4A5	.	stem_loop	149	168	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
pSB4A5	.	sequence_feature	177	183	.	+	0	ID=BBa_G0003;Name=BBa_G0003
pSB4A5	.	terminator	3271	3348	.	+	0	ID=BBa_B0055;Name=BBa_B0055
pSB4A5	.	stem_loop	3306	3327	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
pSB4A5	.	sequence_feature	3298	3336	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
pSB4A5	.	sequence_feature	3374	3395	.	+	0	ID=BBa_G00000;Name=BBa_G00000
pSB4A5	.	sequence_feature	3380	3387	.	+	0	ID=annotation1961228;Name=NotI site;Parent=BBa_G00000
pSB4A5	.	sequence_feature	3374	3379	.	+	0	ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000
pSB4A5	.	engineered_region	3374	3395	.	+	0	ID=annotation1934225;Name=Prefix;Parent=BBa_G00000
pSB4A5	.	sequence_feature	3389	3394	.	+	0	ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000
>pSB4A5
tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg
cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt
tcgctaaggatgatttctgctagagctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaat
gacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcact
agggacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcac
gggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacata
gcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatcca
taaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaaga
cagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaag
cctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgact
aaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataac
cacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttcca
gcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctca
attggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagc
gattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaa
aaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatg
cttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctt
tgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgcct
tatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgagga
tttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaa
gttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagat
ttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaac
tagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccat
tacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagt
tttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaac
gaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcat
caagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaa
cggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgac
aatgtaactactagagctaaatacattcaaatatctatccgctcatgagacaataaccctgataaatgcttcaataata
ttgaaaaaggaagaatatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtt
tttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactgg
acctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgct
ctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttg
gttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataacca
tgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacat
gggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacg
atgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaa
tagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataa
atctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagtt
atctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagc
attggtaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagat
tacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtga
aggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagag