LOCUS pSB4AC5 5827 bp DNA linear 05-May-2024 DEFINITION pSB4AC5-P1011.I50020 ACCESSION pSB4AC5 VERSION pSB4AC5.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 30..34 /label=Nt.BstNB I recognition site misc_feature 35..37 /label=Arbitrary sequence (could be any bases) misc_feature 38..42 /label=Topoisomerase I recognition site misc_feature 38..38 /label=Generated T overhang misc_feature 30..42 /label=BBa_B0044 misc_feature 52..54 /label=stop codon (+2 frame) misc_feature 51..53 /label=stop codon (-1 frame) misc_feature 56..58 /label=stop codon (+3 frame) misc_feature 60..62 /label=stop codon (+1 frame) misc_feature 59..61 /label=stop codon (-2 frame) misc_feature 55..57 /label=stop codon (-3 frame) misc_feature 51..62 /label=BBa_B0042 stem_loop 81..112 /label=stem loop misc_feature 74..113 /label=identical to E. coli MG1655 between tonB and yciA terminator 71..139 /label=BBa_B0054 primer_bind 148..167 /label=VR misc_feature 148..167 /label=BBa_G00102 stem_loop 189..208 /label=stem_loop terminator 176..216 /label=BBa_B0062 CDS 248..907 /label=CmR misc_feature 225..1013 /label=BBa_P1009 stem_loop 1035..1054 /label=stem_loop terminator 1022..1062 /label=BBa_B0062 CDS 1177..1839 /label=cmR misc_feature 1071..2011 /label=BBa_P1004 polyA_site 2095..2110 /label=putative polyA CDS 2221..3171 /label=repA misc_feature 3173..3195 /label=inverted repeat RBS 3173..3178 /label=RBS RBS 3175..3187 /label=RBS promoter 3192..3211 /label=promoter region misc_feature 3198..3219 /label=inverted repeat promoter 3202..3207 /label=rep promoter -10 promoter 3224..3229 /label=rep promoter -35 misc_feature 3238..3243 /label=Mutated SpeI site misc_feature 3269..3288 /label=direct repeat misc_feature 3301..3320 /label=direct repeat misc_feature 3322..3341 /label=direct repeat misc_feature 3340..3422 /label=AT rich region misc_feature 3553..3920 /label=par region misc_feature 3819..3836 /label=inverted repeat misc_feature 3842..3865 /label=inverted repeat misc_feature 3876..3892 /label=inverted repeat misc_feature 3895..3899 /label=Tn3 integration site misc_feature 3905..3918 /label=inverted repeat stem_loop 3982..4011 /label=terminator misc_feature 2055..2055 /label=difference from canonical pSC101 sequence in Genbank misc_feature 2145..2145 /label=difference from canonical pSC101 sequence in Genbank misc_feature 2307..2307 /label=difference from canonical pSC101 sequence in Genbank misc_feature 2402..2402 /label=difference from canonical pSC101 sequence in Genbank misc_feature 2020..4234 /label=BBa_I50041 stem_loop 4251..4285 /label=stem_loop terminator 4243..4314 /label=BBa_B0053 primer_bind 4323..4342 /label=VF2 primer 4323..4342 /label=BBa_G00100 stem_loop 4386..4407 /label=stem loop from bacteriophage T3 misc_feature 4378..4416 /label=identical to bacteriophage T3 terminator 4351..4428 /label=BBa_B0055 misc_feature 4438..4440 /label=stop codon (+2 frame) misc_feature 4437..4439 /label=stop codon (-1 frame) misc_feature 4442..4444 /label=stop codon (+3 frame) misc_feature 4446..4448 /label=stop codon (+1 frame) misc_feature 4445..4447 /label=stop codon (-2 frame) misc_feature 4441..4443 /label=stop codon (-3 frame) misc_feature 4437..4448 /label=BBa_B0042 misc_feature 4457..4461 /label=Topoisomerase I recognition site misc_feature 4465..4469 /label=Nt.BstNB I recognition site misc_feature 4462..4464 /label=Arbitrary sequence (could be any bases) misc_feature 4461..4461 /label=Generated T overhang misc_feature 4457..4469 /label=BBa_B0043 misc_feature 4478..4483 /label=EcoRI site misc_feature 4493..4498 /label=XbaI site misc_feature 4478..4499 /label=Prefix misc_feature 4484..4491 /label=NotI site misc_feature 4478..4499 /label=BBa_G00000 promoter 4548..4553 /label=-35 promoter 4571..4576 /label=-10 CDS 4615..4923 /label=ccdB misc_feature 4508..4961 /label=BBa_P1011 misc_feature 4985..5599 /label=rep (pMB1) misc_feature 5584..5584 /label=ORI misc_feature 4996..5584 /label=origin of replication misc_feature 5032..5584 /label=RNAII transcript promoter 5173..5178 /label=RNAI transcript promoter -35 promoter 5151..5156 /label=RNAI transcript promoter -10 misc_feature 5035..5142 /label=RNAI transcript promoter 5017..5022 /label=RNAII transcript promoter -10 sequence promoter 4996..5001 /label=RNAII transcript promoter -35 sequence misc_feature 5230..5230 /label=G to A, nonfunctional mutation misc_feature 5234..5234 /label=G to A, nonfunctional mutation misc_feature 5243..5243 /label=G to A, nonfunctional mutation misc_feature 5272..5272 /label=C to T, nonfunctional mutation misc_feature 5436..5436 /label=C to T, nonfunctional mutation misc_feature 4970..5827 /label=BBa_I50020 ORIGIN 1 tactagtagc ggccgctgca gtactagagg agtcactaag ggtactagag ttagttagtt 61 agtactagag attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc 121 ccttggggtt atcattgggt actagaggct cactcaaagg cggtaattac tagagcagat 181 aaaaaaaatc cttagctttc gctaaggatg atttcttact agaggtccaa gcgagctcga 241 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc 301 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca 361 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca 421 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa 481 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat 541 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg 601 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca 661 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa 721 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg 781 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct 841 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt 901 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta 961 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactactaga 1021 gcagataaaa aaaatcctta gctttcgcta aggatgattt cttactagag gttaccaatg 1081 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 1141 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 1201 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 1261 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 1321 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 1381 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 1441 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 1501 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 1561 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 1621 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 1681 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 1741 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 1801 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 1861 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 1921 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 1981 catgagcgga tacatatttg aatgtattta gtactagagg tacgggtttt gctgcccgca 2041 aacgggctgt tctggtgttg ctagtttgtt atcagaatcg cagatccggc ttcaggtttg 2101 ccggctgaaa gcgctatttc ttccagaatt gccatgattt tttccccacg ggaggcgtca 2161 ctggctcccg tgttgtcggc agctttgatt cgataagcag catcgcctgt ttcaggctgt 2221 ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca tgttctagtt 2281 gctttgtttt actggtttca cctgttctat taggtgttac atgctgttca tctgttacat 2341 tgtcgatctg ttcatggtga acagctttaa atgcaccaaa aactcgtaaa agctctgatg 2401 tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc tttgatatct 2461 aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa 2521 gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt 2581 tgtttttgcg tgagccatga gaacgaacca ttgagatcat gcttactttg catgtcactc 2641 aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt 2701 tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca 2761 ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca 2821 acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg 2881 ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac 2941 tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata 3001 tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata 3061 gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac 3121 tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac 3181 ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc 3241 acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc attttcccta 3301 ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt 3361 gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca 3421 tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca 3481 gacatacatc tcaattggtc taggtgattt taatcactat accaattgag atgggctagt 3541 caatgataat tacatgtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc 3601 tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt 3661 gttttttttg tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag 3721 ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat 3781 tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa 3841 ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt tgtctccgac 3901 catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc tcacggctct 3961 ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg caaggaaact 4021 acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg cgggtctgct 4081 atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt ttccagtctg 4141 accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc agtaaggcag 4201 cggtatcatc aacaggctta cccgtcttac tgtctactag agtccggcaa aaaaacgggc 4261 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgtttactag 4321 agtgccacct gacgtctaag aatactagag aaggaatatt cagcaatttg cccgtgccga 4381 agaaaggccc acccgtgaag gtgagccagt gagttgattg ctacgtaata ctagagttag 4441 ttagttagta ctagagccct tagtgactct actagaggaa ttcgcggccg cttctagagt 4501 actagagacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc 4561 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg 4621 tatgctaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt 4681 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag 4741 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga 4801 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga 4861 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt 4921 ctggggaata taaatgtcag gctcccttat acacagccag ttactagagc tgtcagacca 4981 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 5041 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 5101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 5161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 5221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 5281 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 5341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 5401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 5461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 5521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 5581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 5641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 5701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 5761 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 5821 taaccgt //