pSB4AC5
type: ComponentDefinition
persistent identity: pSB4AC5
display id: pSB4AC5
version: 1
was generated by: iGEM to SBOL conversion
description: pSB4AC5-P1011.I50020
created: 2006-03-17T12:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Low copy vector designed entirely from BioBricks parts. See http://openwetware.org/wiki/Synthetic_Biology:Vectors/Wishlist for details.
discontinued: true
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
component: BBa_I50041
component: BBa_P1004
component: BBa_B0062
component: BBa_B0043
component: BBa_B0042
component: BBa_B0055
component: BBa_G00100
component: BBa_I50020
component: BBa_P1011
component: BBa_G00000
component: BBa_B0044
component: BBa_G00001
component: BBa_P1009
component: BBa_G00102
component: BBa_B0054
component: BBa_B0053
sequence annotation: BBa_G00001
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_G00102
sequence annotation: BBa_B0062
sequence annotation: BBa_P1009
sequence annotation: BBa_P1004
sequence annotation: BBa_I50041
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_B0043
sequence annotation: BBa_G00000
sequence annotation: BBa_P1011
sequence annotation: BBa_I50020
sequence: https://synbiohub.org/public/igem/pSB4AC5_sequence/1

BBa_I50041
type: Component
persistent identity: component1880259
display id: component1880259
version: 1
top level: pSB4AC5
definition: pSC101
access: public

BBa_P1004
type: Component
persistent identity: component1880240
display id: component1880240
version: 1
top level: pSB4AC5
definition: cmR
access: public

BBa_B0062
type: Component
persistent identity: component1880238
display id: component1880238
version: 1
top level: pSB4AC5
definition: BBa_B0062
access: public

BBa_B0043
type: Component
persistent identity: component1880277
display id: component1880277
version: 1
top level: pSB4AC5
definition: TOPO
access: public

BBa_B0042
type: Component
persistent identity: component1880272
display id: component1880272
version: 1
top level: pSB4AC5
definition: TSS
access: public

BBa_B0055
type: Component
persistent identity: component1880265
display id: component1880265
version: 1
top level: pSB4AC5
definition: BBa_B0055
access: public

BBa_G00100
type: Component
persistent identity: component1880262
display id: component1880262
version: 1
top level: pSB4AC5
definition: VF2
access: public

BBa_I50020
type: Component
persistent identity: component1880293
display id: component1880293
version: 1
top level: pSB4AC5
definition: pUC19 ori
access: public

BBa_P1011
type: Component
persistent identity: component1880283
display id: component1880283
version: 1
top level: pSB4AC5
definition: ccdB
access: public

BBa_G00000
type: Component
persistent identity: component1880279
display id: component1880279
version: 1
top level: pSB4AC5
definition: Prefix
access: public

BBa_B0042
type: Component
persistent identity: component1880228
display id: component1880228
version: 1
top level: pSB4AC5
definition: TSS
access: public

BBa_B0044
type: Component
persistent identity: component1880221
display id: component1880221
version: 1
top level: pSB4AC5
definition: TOPO
access: public

BBa_G00001
type: Component
persistent identity: component1880216
display id: component1880216
version: 1
top level: pSB4AC5
definition: Suffix
access: public

BBa_P1009
type: Component
persistent identity: component1880236
display id: component1880236
version: 1
top level: pSB4AC5
definition: BBa_P1009
access: public

BBa_B0062
type: Component
persistent identity: component1880234
display id: component1880234
version: 1
top level: pSB4AC5
definition: BBa_B0062
access: public

BBa_G00102
type: Component
persistent identity: component1880232
display id: component1880232
version: 1
top level: pSB4AC5
definition: VR
access: public

BBa_B0054
type: Component
persistent identity: component1880231
display id: component1880231
version: 1
top level: pSB4AC5
definition: BBa_B0054
access: public

BBa_B0053
type: Component
persistent identity: component1880261
display id: component1880261
version: 1
top level: pSB4AC5
definition: BBa_B0053
access: public

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1880216
display id: annotation1880216
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880216/range1880216/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4AC5/annotation1880216/range1880216/1
type: Range
persistent identity: range1880216
display id: range1880216
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 1
end: 21
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1880221
display id: annotation1880221
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880221/range1880221/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4AC5/annotation1880221/range1880221/1
type: Range
persistent identity: range1880221
display id: range1880221
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 30
end: 42
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1880228
display id: annotation1880228
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880228/range1880228/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4AC5/annotation1880228/range1880228/1
type: Range
persistent identity: range1880228
display id: range1880228
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 51
end: 62
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1880231
display id: annotation1880231
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880231/range1880231/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4AC5/annotation1880231/range1880231/1
type: Range
persistent identity: range1880231
display id: range1880231
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 71
end: 139
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1880232
display id: annotation1880232
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880232/range1880232/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4AC5/annotation1880232/range1880232/1
type: Range
persistent identity: range1880232
display id: range1880232
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 148
end: 167
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1880234
display id: annotation1880234
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880234/range1880234/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4AC5/annotation1880234/range1880234/1
type: Range
persistent identity: range1880234
display id: range1880234
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 176
end: 216
orientation: inline

BBa_P1009
type: SequenceAnnotation
persistent identity: annotation1880236
display id: annotation1880236
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880236/range1880236/1
component: BBa_P1009

https://synbiohub.org/public/igem/pSB4AC5/annotation1880236/range1880236/1
type: Range
persistent identity: range1880236
display id: range1880236
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 225
end: 1013
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1880238
display id: annotation1880238
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880238/range1880238/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4AC5/annotation1880238/range1880238/1
type: Range
persistent identity: range1880238
display id: range1880238
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 1022
end: 1062
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation1880240
display id: annotation1880240
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880240/range1880240/1
component: BBa_P1004

https://synbiohub.org/public/igem/pSB4AC5/annotation1880240/range1880240/1
type: Range
persistent identity: range1880240
display id: range1880240
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 1071
end: 2011
orientation: inline

BBa_I50041
type: SequenceAnnotation
persistent identity: annotation1880259
display id: annotation1880259
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880259/range1880259/1
component: BBa_I50041

https://synbiohub.org/public/igem/pSB4AC5/annotation1880259/range1880259/1
type: Range
persistent identity: range1880259
display id: range1880259
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 2020
end: 4234
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1880261
display id: annotation1880261
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880261/range1880261/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4AC5/annotation1880261/range1880261/1
type: Range
persistent identity: range1880261
display id: range1880261
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 4243
end: 4314
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1880262
display id: annotation1880262
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880262/range1880262/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4AC5/annotation1880262/range1880262/1
type: Range
persistent identity: range1880262
display id: range1880262
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 4323
end: 4342
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1880265
display id: annotation1880265
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880265/range1880265/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4AC5/annotation1880265/range1880265/1
type: Range
persistent identity: range1880265
display id: range1880265
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 4351
end: 4428
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1880272
display id: annotation1880272
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880272/range1880272/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4AC5/annotation1880272/range1880272/1
type: Range
persistent identity: range1880272
display id: range1880272
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 4437
end: 4448
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1880277
display id: annotation1880277
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880277/range1880277/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4AC5/annotation1880277/range1880277/1
type: Range
persistent identity: range1880277
display id: range1880277
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 4457
end: 4469
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1880279
display id: annotation1880279
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880279/range1880279/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4AC5/annotation1880279/range1880279/1
type: Range
persistent identity: range1880279
display id: range1880279
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 4478
end: 4499
orientation: inline

BBa_P1011
type: SequenceAnnotation
persistent identity: annotation1880283
display id: annotation1880283
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880283/range1880283/1
component: BBa_P1011

https://synbiohub.org/public/igem/pSB4AC5/annotation1880283/range1880283/1
type: Range
persistent identity: range1880283
display id: range1880283
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 4508
end: 4961
orientation: inline

BBa_I50020
type: SequenceAnnotation
persistent identity: annotation1880293
display id: annotation1880293
version: 1
top level: pSB4AC5
location: https://synbiohub.org/public/igem/pSB4AC5/annotation1880293/range1880293/1
component: BBa_I50020

https://synbiohub.org/public/igem/pSB4AC5/annotation1880293/range1880293/1
type: Range
persistent identity: range1880293
display id: range1880293
version: 1
top level: pSB4AC5
direction: direction/reverse
start: 4970
end: 5827
orientation: inline

pSC101
type: ComponentDefinition
persistent identity: BBa I50041
display id: BBa_I50041
version: 1
was generated by: iGEM to SBOL conversion
description: pSC101 origin of replication, reverse
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:42Z
owned by: james
owned by: myers
mutable description: pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. This part is the reverse copy of BBa_I50040.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: difference from canonical pSC101 sequence in Genbank
sequence annotation: putative polyA
sequence annotation: repA
sequence annotation: RBS
sequence annotation: inverted repeat
sequence annotation: promoter region
sequence annotation: rep promoter -10
sequence annotation: rep promoter -35
sequence annotation: Mutated SpeI site
sequence annotation: direct repeat
sequence annotation: AT rich region
sequence annotation: par region
sequence annotation: Tn3 integration site
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_I50041_sequence/1

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080856
display id: annotation2080856
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080856/range2080856/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50041/annotation2080856/range2080856/1
type: Range
persistent identity: range2080856
display id: range2080856
version: 1
top level: pSC101
direction: direction/forward
start: 36
end: 36
orientation: inline

putative polyA
type: SequenceAnnotation
persistent identity: annotation1932215
display id: annotation1932215
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932215/range1932215/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_I50041/annotation1932215/range1932215/1
type: Range
persistent identity: range1932215
display id: range1932215
version: 1
top level: pSC101
direction: direction/reverse
start: 76
end: 91
orientation: inline

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080857
display id: annotation2080857
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080857/range2080857/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50041/annotation2080857/range2080857/1
type: Range
persistent identity: range2080857
display id: range2080857
version: 1
top level: pSC101
direction: direction/forward
start: 126
end: 126
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation1932216
display id: annotation1932216
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932216/range1932216/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I50041/annotation1932216/range1932216/1
type: Range
persistent identity: range1932216
display id: range1932216
version: 1
top level: pSC101
direction: direction/reverse
start: 202
end: 1152
orientation: inline

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080858
display id: annotation2080858
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080858/range2080858/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50041/annotation2080858/range2080858/1
type: Range
persistent identity: range2080858
display id: range2080858
version: 1
top level: pSC101
direction: direction/forward
start: 288
end: 288
orientation: inline

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080859
display id: annotation2080859
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080859/range2080859/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50041/annotation2080859/range2080859/1
type: Range
persistent identity: range2080859
display id: range2080859
version: 1
top level: pSC101
direction: direction/forward
start: 383
end: 383
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1932218
display id: annotation1932218
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932218/range1932218/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I50041/annotation1932218/range1932218/1
type: Range
persistent identity: range1932218
display id: range1932218
version: 1
top level: pSC101
direction: direction/reverse
start: 1154
end: 1159
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932217
display id: annotation1932217
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932217/range1932217/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50041/annotation1932217/range1932217/1
type: Range
persistent identity: range1932217
display id: range1932217
version: 1
top level: pSC101
direction: direction/reverse
start: 1154
end: 1176
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1932219
display id: annotation1932219
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932219/range1932219/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I50041/annotation1932219/range1932219/1
type: Range
persistent identity: range1932219
display id: range1932219
version: 1
top level: pSC101
direction: direction/reverse
start: 1156
end: 1168
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation1932220
display id: annotation1932220
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932220/range1932220/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I50041/annotation1932220/range1932220/1
type: Range
persistent identity: range1932220
display id: range1932220
version: 1
top level: pSC101
direction: direction/reverse
start: 1173
end: 1192
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932221
display id: annotation1932221
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932221/range1932221/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50041/annotation1932221/range1932221/1
type: Range
persistent identity: range1932221
display id: range1932221
version: 1
top level: pSC101
direction: direction/reverse
start: 1179
end: 1200
orientation: inline

rep promoter -10
type: SequenceAnnotation
persistent identity: annotation1932222
display id: annotation1932222
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932222/range1932222/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50041/annotation1932222/range1932222/1
type: Range
persistent identity: range1932222
display id: range1932222
version: 1
top level: pSC101
direction: direction/reverse
start: 1183
end: 1188
orientation: inline

rep promoter -35
type: SequenceAnnotation
persistent identity: annotation1932223
display id: annotation1932223
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932223/range1932223/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50041/annotation1932223/range1932223/1
type: Range
persistent identity: range1932223
display id: range1932223
version: 1
top level: pSC101
direction: direction/reverse
start: 1205
end: 1210
orientation: inline

Mutated SpeI site
type: SequenceAnnotation
persistent identity: annotation1932224
display id: annotation1932224
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932224/range1932224/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50041/annotation1932224/range1932224/1
type: Range
persistent identity: range1932224
display id: range1932224
version: 1
top level: pSC101
direction: direction/reverse
start: 1219
end: 1224
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1932225
display id: annotation1932225
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932225/range1932225/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50041/annotation1932225/range1932225/1
type: Range
persistent identity: range1932225
display id: range1932225
version: 1
top level: pSC101
direction: direction/reverse
start: 1250
end: 1269
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1932226
display id: annotation1932226
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932226/range1932226/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50041/annotation1932226/range1932226/1
type: Range
persistent identity: range1932226
display id: range1932226
version: 1
top level: pSC101
direction: direction/reverse
start: 1282
end: 1301
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1932227
display id: annotation1932227
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932227/range1932227/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50041/annotation1932227/range1932227/1
type: Range
persistent identity: range1932227
display id: range1932227
version: 1
top level: pSC101
direction: direction/reverse
start: 1303
end: 1322
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation1932228
display id: annotation1932228
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932228/range1932228/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50041/annotation1932228/range1932228/1
type: Range
persistent identity: range1932228
display id: range1932228
version: 1
top level: pSC101
direction: direction/reverse
start: 1321
end: 1403
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation1932229
display id: annotation1932229
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932229/range1932229/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50041/annotation1932229/range1932229/1
type: Range
persistent identity: range1932229
display id: range1932229
version: 1
top level: pSC101
direction: direction/reverse
start: 1534
end: 1901
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932230
display id: annotation1932230
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932230/range1932230/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50041/annotation1932230/range1932230/1
type: Range
persistent identity: range1932230
display id: range1932230
version: 1
top level: pSC101
direction: direction/reverse
start: 1800
end: 1817
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932231
display id: annotation1932231
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932231/range1932231/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50041/annotation1932231/range1932231/1
type: Range
persistent identity: range1932231
display id: range1932231
version: 1
top level: pSC101
direction: direction/reverse
start: 1823
end: 1846
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932232
display id: annotation1932232
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932232/range1932232/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50041/annotation1932232/range1932232/1
type: Range
persistent identity: range1932232
display id: range1932232
version: 1
top level: pSC101
direction: direction/reverse
start: 1857
end: 1873
orientation: inline

Tn3 integration site
type: SequenceAnnotation
persistent identity: annotation1932233
display id: annotation1932233
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932233/range1932233/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50041/annotation1932233/range1932233/1
type: Range
persistent identity: range1932233
display id: range1932233
version: 1
top level: pSC101
direction: direction/reverse
start: 1876
end: 1880
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932234
display id: annotation1932234
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932234/range1932234/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50041/annotation1932234/range1932234/1
type: Range
persistent identity: range1932234
display id: range1932234
version: 1
top level: pSC101
direction: direction/reverse
start: 1886
end: 1899
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1932235
display id: annotation1932235
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932235/range1932235/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_I50041/annotation1932235/range1932235/1
type: Range
persistent identity: range1932235
display id: range1932235
version: 1
top level: pSC101
direction: direction/reverse
start: 1963
end: 1992
orientation: inline

BBa_P1009
type: ComponentDefinition
persistent identity: BBa P1009
display id: BBa_P1009
version: 1
was generated by: iGEM to SBOL conversion
description: CmR (reverse)
created: 2006-05-31T11:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_P1009_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1877871
display id: annotation1877871
version: 1
top level: BBa_P1009
location: https://synbiohub.org/public/igem/BBa_P1009/annotation1877871/range1877871/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1009/annotation1877871/range1877871/1
type: Range
persistent identity: range1877871
display id: range1877871
version: 1
top level: BBa_P1009
direction: direction/reverse
start: 24
end: 683
orientation: inline

TSS
type: ComponentDefinition
persistent identity: BBa B0042
display id: BBa_B0042
version: 1
was generated by: iGEM to SBOL conversion
description: Translational stop sequence
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: stop codon (-1 frame)
sequence annotation: stop codon (+2 frame)
sequence annotation: stop codon (-3 frame)
sequence annotation: stop codon (+3 frame)
sequence annotation: stop codon (-2 frame)
sequence annotation: stop codon (+1 frame)
sequence: https://synbiohub.org/public/igem/BBa_B0042_sequence/1

stop codon (-1 frame)
type: SequenceAnnotation
persistent identity: annotation1797084
display id: annotation1797084
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
type: Range
persistent identity: range1797084
display id: range1797084
version: 1
top level: TSS
direction: direction/reverse
start: 1
end: 3
orientation: inline

stop codon (+2 frame)
type: SequenceAnnotation
persistent identity: annotation1797083
display id: annotation1797083
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
type: Range
persistent identity: range1797083
display id: range1797083
version: 1
top level: TSS
direction: direction/forward
start: 2
end: 4
orientation: inline

stop codon (-3 frame)
type: SequenceAnnotation
persistent identity: annotation1797088
display id: annotation1797088
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
type: Range
persistent identity: range1797088
display id: range1797088
version: 1
top level: TSS
direction: direction/reverse
start: 5
end: 7
orientation: inline

stop codon (+3 frame)
type: SequenceAnnotation
persistent identity: annotation1797085
display id: annotation1797085
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
type: Range
persistent identity: range1797085
display id: range1797085
version: 1
top level: TSS
direction: direction/forward
start: 6
end: 8
orientation: inline

stop codon (-2 frame)
type: SequenceAnnotation
persistent identity: annotation1797087
display id: annotation1797087
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
type: Range
persistent identity: range1797087
display id: range1797087
version: 1
top level: TSS
direction: direction/reverse
start: 9
end: 11
orientation: inline

stop codon (+1 frame)
type: SequenceAnnotation
persistent identity: annotation1797086
display id: annotation1797086
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
type: Range
persistent identity: range1797086
display id: range1797086
version: 1
top level: TSS
direction: direction/forward
start: 10
end: 12
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0043
display id: BBa_B0043
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (forward)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Nt.BstNB I recognition site
sequence: https://synbiohub.org/public/igem/BBa_B0043_sequence/1

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797101
display id: annotation1797101
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
type: Range
persistent identity: range1797101
display id: range1797101
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797104
display id: annotation1797104
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
type: Range
persistent identity: range1797104
display id: range1797104
version: 1
top level: TOPO
direction: direction/forward
start: 5
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797103
display id: annotation1797103
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
type: Range
persistent identity: range1797103
display id: range1797103
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797102
display id: annotation1797102
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
type: Range
persistent identity: range1797102
display id: range1797102
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0044
display id: BBa_B0044
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (reverse)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Nt.BstNB I recognition site
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence: https://synbiohub.org/public/igem/BBa_B0044_sequence/1

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797126
display id: annotation1797126
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
type: Range
persistent identity: range1797126
display id: range1797126
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797127
display id: annotation1797127
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
type: Range
persistent identity: range1797127
display id: range1797127
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797128
display id: annotation1797128
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
type: Range
persistent identity: range1797128
display id: range1797128
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797129
display id: annotation1797129
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
type: Range
persistent identity: range1797129
display id: range1797129
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 9
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to bacteriophage T3
sequence annotation: stem loop from bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 28
end: 66
orientation: inline

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 36
end: 57
orientation: inline

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0052)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
top level: BBa_B0062
direction: direction/forward
start: 14
end: 33
orientation: inline

Prefix
type: ComponentDefinition
persistent identity: BBa G00000
display id: BBa_G00000
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site prefix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks prefix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Prefix
sequence annotation: EcoRI site
sequence annotation: NotI site
sequence annotation: XbaI site
sequence: https://synbiohub.org/public/igem/BBa_G00000_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation1934225
display id: annotation1934225
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
type: Range
persistent identity: range1934225
display id: range1934225
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 22
orientation: inline

EcoRI site
type: SequenceAnnotation
persistent identity: annotation1934223
display id: annotation1934223
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
type: Range
persistent identity: range1934223
display id: range1934223
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 6
orientation: inline

NotI site
type: SequenceAnnotation
persistent identity: annotation1961228
display id: annotation1961228
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
type: Range
persistent identity: range1961228
display id: range1961228
version: 1
top level: Prefix
direction: direction/forward
start: 7
end: 14
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation1934224
display id: annotation1934224
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
type: Range
persistent identity: range1934224
display id: range1934224
version: 1
top level: Prefix
direction: direction/forward
start: 16
end: 21
orientation: inline

Suffix
type: ComponentDefinition
persistent identity: BBa G00001
display id: BBa_G00001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site suffix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks suffix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Suffix
sequence annotation: SpeI
sequence annotation: NotI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_G00001_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation1821544
display id: annotation1821544
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
type: Range
persistent identity: range1821544
display id: range1821544
version: 1
top level: Suffix
direction: direction/forward
start: 1
end: 21
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1934226
display id: annotation1934226
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
type: Range
persistent identity: range1934226
display id: range1934226
version: 1
top level: Suffix
direction: direction/forward
start: 2
end: 7
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1934228
display id: annotation1934228
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
type: Range
persistent identity: range1934228
display id: range1934228
version: 1
top level: Suffix
direction: direction/forward
start: 9
end: 16
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1934227
display id: annotation1934227
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
type: Range
persistent identity: range1934227
display id: range1934227
version: 1
top level: Suffix
direction: direction/forward
start: 16
end: 21
orientation: inline

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

VR
type: ComponentDefinition
persistent identity: BBa G00102
display id: BBa_G00102
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick primer annealing site (VR binding site)
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00102_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1961229
display id: annotation1961229
version: 1
top level: VR
location: https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
type: Range
persistent identity: range1961229
display id: range1961229
version: 1
top level: VR
direction: direction/reverse
start: 1
end: 20
orientation: inline

pUC19 ori
type: ComponentDefinition
persistent identity: BBa I50020
display id: BBa_I50020
version: 1
was generated by: iGEM to SBOL conversion
description: Minimal pUC19-derived high copy replication origin
created: 2006-01-09T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: rep (pMB1)
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: origin of replication
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: RNAII transcript
sequence annotation: RNAI transcript
sequence annotation: RNAI transcript promoter -10
sequence annotation: RNAI transcript promoter -35
sequence annotation: G to A, nonfunctional mutation
sequence annotation: C to T, nonfunctional mutation
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_I50020_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1782180
display id: annotation1782180
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1782180/range1782180/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50020/annotation1782180/range1782180/1
type: Range
persistent identity: range1782180
display id: range1782180
version: 1
top level: pUC19 ori
direction: direction/forward
start: 16
end: 630
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1793686
display id: annotation1793686
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793686/range1793686/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793686/range1793686/1
type: Range
persistent identity: range1793686
display id: range1793686
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 32
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1793680
display id: annotation1793680
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793680/range1793680/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50020/annotation1793680/range1793680/1
type: Range
persistent identity: range1793680
display id: range1793680
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 615
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1793685
display id: annotation1793685
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793685/range1793685/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793685/range1793685/1
type: Range
persistent identity: range1793685
display id: range1793685
version: 1
top level: pUC19 ori
direction: direction/forward
start: 48
end: 53
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1793681
display id: annotation1793681
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793681/range1793681/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50020/annotation1793681/range1793681/1
type: Range
persistent identity: range1793681
display id: range1793681
version: 1
top level: pUC19 ori
direction: direction/forward
start: 63
end: 615
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1793684
display id: annotation1793684
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793684/range1793684/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50020/annotation1793684/range1793684/1
type: Range
persistent identity: range1793684
display id: range1793684
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 66
end: 173
orientation: inline

RNAI transcript promoter -10
type: SequenceAnnotation
persistent identity: annotation1793683
display id: annotation1793683
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793683/range1793683/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793683/range1793683/1
type: Range
persistent identity: range1793683
display id: range1793683
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 182
end: 187
orientation: inline

RNAI transcript promoter -35
type: SequenceAnnotation
persistent identity: annotation1793682
display id: annotation1793682
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793682/range1793682/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793682/range1793682/1
type: Range
persistent identity: range1793682
display id: range1793682
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 204
end: 209
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910687
display id: annotation1910687
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910687/range1910687/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910687/range1910687/1
type: Range
persistent identity: range1910687
display id: range1910687
version: 1
top level: pUC19 ori
direction: direction/forward
start: 261
end: 261
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910688
display id: annotation1910688
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910688/range1910688/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910688/range1910688/1
type: Range
persistent identity: range1910688
display id: range1910688
version: 1
top level: pUC19 ori
direction: direction/forward
start: 265
end: 265
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910689
display id: annotation1910689
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910689/range1910689/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910689/range1910689/1
type: Range
persistent identity: range1910689
display id: range1910689
version: 1
top level: pUC19 ori
direction: direction/forward
start: 274
end: 274
orientation: inline

C to T, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910690
display id: annotation1910690
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910690/range1910690/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910690/range1910690/1
type: Range
persistent identity: range1910690
display id: range1910690
version: 1
top level: pUC19 ori
direction: direction/forward
start: 303
end: 303
orientation: inline

C to T, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910691
display id: annotation1910691
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910691/range1910691/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910691/range1910691/1
type: Range
persistent identity: range1910691
display id: range1910691
version: 1
top level: pUC19 ori
direction: direction/forward
start: 467
end: 467
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1782181
display id: annotation1782181
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1782181/range1782181/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50020/annotation1782181/range1782181/1
type: Range
persistent identity: range1782181
display id: range1782181
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 615
end: 615
orientation: inline

cmR
type: ComponentDefinition
persistent identity: BBa P1004
display id: BBa_P1004
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol resistance cassette
created: 2006-02-03T12:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same part as BBa_P1002 except in reverse orientation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: cmR
sequence: https://synbiohub.org/public/igem/BBa_P1004_sequence/1

cmR
type: SequenceAnnotation
persistent identity: annotation1897114
display id: annotation1897114
version: 1
top level: cmR
location: https://synbiohub.org/public/igem/BBa_P1004/annotation1897114/range1897114/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_P1004/annotation1897114/range1897114/1
type: Range
persistent identity: range1897114
display id: range1897114
version: 1
top level: cmR
direction: direction/forward
start: 107
end: 769
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa P1011
display id: BBa_P1011
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB cassette
created: 2006-03-13T12:00:00Z
modified: 2015-07-09T11:40:28Z
owned by: james
owned by: myers
mutable description: This is identical to BBa_P1010 except that ccdA- has been deleted since its presence is not necessary for ccdB's function as a toxin.
discontinued: true
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 4206
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Deleted
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: -35
sequence annotation: -10
sequence annotation: ccdB
sequence: https://synbiohub.org/public/igem/BBa_P1011_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1822230
display id: annotation1822230
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1011/annotation1822230/range1822230/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1011/annotation1822230/range1822230/1
type: Range
persistent identity: range1822230
display id: range1822230
version: 1
top level: ccdB
direction: direction/forward
start: 41
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1822231
display id: annotation1822231
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1011/annotation1822231/range1822231/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1011/annotation1822231/range1822231/1
type: Range
persistent identity: range1822231
display id: range1822231
version: 1
top level: ccdB
direction: direction/forward
start: 64
end: 69
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation1822232
display id: annotation1822232
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1011/annotation1822232/range1822232/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1011/annotation1822232/range1822232/1
type: Range
persistent identity: range1822232
display id: range1822232
version: 1
top level: ccdB
direction: direction/forward
start: 108
end: 416
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

https://synbiohub.org/public/igem/pSB4AC5_sequence/1
type: Sequence
persistent identity: pSB4AC5 sequence
display id: pSB4AC5_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtactagaggagtcactaagggtactagagttagttagttagtactagagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattgggtactagaggctcactcaaaggcggtaattactagagcagataaaaaaaatccttagctttcgctaaggatgatttcttactagaggtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactactagagcagataaaaaaaatccttagctttcgctaaggatgatttcttactagaggttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50041_sequence/1
type: Sequence
persistent identity: BBa I50041 sequence
display id: BBa_I50041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttacattgtcgatctgttcatggtgaacagctttgaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1009_sequence/1
type: Sequence
persistent identity: BBa P1009 sequence
display id: BBa_P1009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0042_sequence/1
type: Sequence
persistent identity: BBa B0042 sequence
display id: BBa_B0042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagttagttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0043_sequence/1
type: Sequence
persistent identity: BBa B0043 sequence
display id: BBa_B0043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttagtgactc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0044_sequence/1
type: Sequence
persistent identity: BBa B0044 sequence
display id: BBa_B0044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagtcactaaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0055_sequence/1
type: Sequence
persistent identity: BBa B0055 sequence
display id: BBa_B0055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0062_sequence/1
type: Sequence
persistent identity: BBa B0062 sequence
display id: BBa_B0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagataaaaaaaatccttagctttcgctaaggatgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00000_sequence/1
type: Sequence
persistent identity: BBa G00000 sequence
display id: BBa_G00000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00001_sequence/1
type: Sequence
persistent identity: BBa G00001 sequence
display id: BBa_G00001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00102_sequence/1
type: Sequence
persistent identity: BBa G00102 sequence
display id: BBa_G00102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcactcaaaggcggtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50020_sequence/1
type: Sequence
persistent identity: BBa I50020 sequence
display id: BBa_I50020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1004_sequence/1
type: Sequence
persistent identity: BBa P1004 sequence
display id: BBa_P1004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttgatcgggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1011_sequence/1
type: Sequence
persistent identity: BBa P1011 sequence
display id: BBa_P1011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaacgtgagagccgttatcgtctgtttgtggacgtacagagtgatattattgacacgcctgggcgtcgcatggtgattcccctggccagcgcacgtctgctttcagataaagtctcccgtgaactttacccggtggtgcatatcggggacgaaagctggcgcatgatgaccaccgatatggccagcgtgccggtttccgtgatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z