BBa_I51001 BioBricks vector scaffold, pSC101 based origin and a version of the chloramphenicol rsistance cassette that has been cleaned of restriction sites.
pSB4C3 is a low copy plasmid based with chloramphenicol resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) pSB4C5 is derived from the BioBricks vector scaffold BBa_I51001. However, the direction of the resistance marker and replication origin is not optimal.
Topoisomerase I mediated cloning site (reverse)
Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.
This part is the standard BioBricks suffix.
This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part.
Topoisomerase I mediated cloning site (forward)
Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
identical to E. coli MG1655 between tonB and yciA
Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
This part is the standard BioBricks prefix.
Forward primer for sequencing/amplifying BioBrick parts (VF2)
Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
Reverse BioBrick primer annealing site (VR binding site)
This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
same part as BBa_P1002 except in reverse orientation.
This part was amplified from pSB4A3-P1010.
pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids.
The replication origin boundaries were chosen quite liberally (i.e. they might include more sequence than is strictly necessary) because a different version of this origin (BBa_I50040) was shown to be non-functional.
G to A difference with canonical pSC101 vector sequence
C to T difference with canonical pSC101 vector sequence
G to C difference with canonical pSC101 vector sequence
T to C difference with canonical pSC101 vector sequence
XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
NheI/SpeI mixed site. Used to help enter parts into the registry.
Conversion of the iGEM parts registry to SBOL2.1