##gff-version 3
##sequence-region pSB5A5 1 7012
pSB5A5	.	engineered_region	225	993	.	+	0	ID=BBa_P1004;Name=BBa_P1004
pSB5A5	.	CDS	331	993	.	+	0	ID=annotation1897114;Name=cmR;Parent=BBa_P1004
pSB5A5	.	terminator	5758	5835	.	+	0	ID=BBa_B0055;Name=BBa_B0055
pSB5A5	.	sequence_feature	5785	5823	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
pSB5A5	.	stem_loop	5793	5814	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
pSB5A5	.	engineered_region	5915	7012	.	+	0	ID=BBa_I52000;Name=BBa_I52000
pSB5A5	.	plasmid	6339	7012	.	+	0	ID=BBa_I50020;Name=BBa_I50020;Parent=BBa_I52000
pSB5A5	.	sequence_feature	6404	6511	.	+	0	ID=annotation1793684;Name=RNAI transcript;Parent=BBa_I50020
pSB5A5	.	sequence_alteration	6603	6603	.	+	0	ID=annotation1910688;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
pSB5A5	.	promoter	6386	6391	.	+	0	ID=annotation1793685;Name=RNAII transcript promoter -10 sequence;Parent=BBa_I50020
pSB5A5	.	sequence_feature	6354	6968	.	+	0	ID=annotation1782180;Name=rep (pMB1);Parent=BBa_I50020
pSB5A5	.	sequence_alteration	6599	6599	.	+	0	ID=annotation1910687;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
pSB5A5	.	sequence_feature	6953	6953	.	+	0	ID=annotation1782181;Name=ORI;Parent=BBa_I50020
pSB5A5	.	sequence_feature	6365	6953	.	+	0	ID=annotation1793680;Name=origin of replication;Parent=BBa_I50020
pSB5A5	.	sequence_alteration	6612	6612	.	+	0	ID=annotation1910689;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
pSB5A5	.	sequence_alteration	6805	6805	.	+	0	ID=annotation1910691;Name=C to T, nonfunctional mutation;Parent=BBa_I50020
pSB5A5	.	promoter	6542	6547	.	+	0	ID=annotation1793682;Name=RNAI transcript promoter -35;Parent=BBa_I50020
pSB5A5	.	sequence_alteration	6641	6641	.	+	0	ID=annotation1910690;Name=C to T, nonfunctional mutation;Parent=BBa_I50020
pSB5A5	.	promoter	6520	6525	.	+	0	ID=annotation1793683;Name=RNAI transcript promoter -10;Parent=BBa_I50020
pSB5A5	.	sequence_feature	6401	6953	.	+	0	ID=annotation1793681;Name=RNAII transcript;Parent=BBa_I50020
pSB5A5	.	promoter	6365	6370	.	+	0	ID=annotation1793686;Name=RNAII transcript promoter -35 sequence;Parent=BBa_I50020
pSB5A5	.	engineered_region	5915	6330	.	+	0	ID=BBa_P1011;Name=BBa_P1011;Parent=BBa_I52000
pSB5A5	.	CDS	6022	6330	.	+	0	ID=annotation1822232;Name=ccdB;Parent=BBa_P1011
pSB5A5	.	promoter	5955	5960	.	+	0	ID=annotation1822230;Name=-35;Parent=BBa_P1011
pSB5A5	.	promoter	5978	5983	.	+	0	ID=annotation1822231;Name=-10;Parent=BBa_P1011
pSB5A5	.	sequence_feature	51	62	.	+	0	ID=BBa_B0042;Name=BBa_B0042
pSB5A5	.	stop_codon	56	58	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
pSB5A5	.	stop_codon	51	53	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
pSB5A5	.	stop_codon	52	54	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
pSB5A5	.	stop_codon	60	62	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
pSB5A5	.	stop_codon	59	61	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
pSB5A5	.	stop_codon	55	57	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
pSB5A5	.	terminator	71	139	.	+	0	ID=BBa_B0054;Name=BBa_B0054
pSB5A5	.	sequence_feature	74	113	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
pSB5A5	.	stem_loop	81	112	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
pSB5A5	.	sequence_feature	30	42	.	+	0	ID=BBa_B0044;Name=BBa_B0044
pSB5A5	.	sequence_feature	38	38	.	+	0	ID=annotation1797129;Name=Generated T overhang;Parent=BBa_B0044
pSB5A5	.	sequence_feature	35	37	.	+	0	ID=annotation1797127;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0044
pSB5A5	.	sequence_feature	38	42	.	+	0	ID=annotation1797128;Name=Topoisomerase I recognition site;Parent=BBa_B0044
pSB5A5	.	sequence_feature	30	34	.	+	0	ID=annotation1797126;Name=Nt.BstNB I recognition site;Parent=BBa_B0044
pSB5A5	.	primer	5730	5749	.	+	0	ID=BBa_G00100;Name=BBa_G00100
pSB5A5	.	primer_binding_site	5730	5749	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
pSB5A5	.	terminator	176	216	.	+	0	ID=BBa_B0062;Name=BBa_B0062
pSB5A5	.	stem_loop	189	208	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
pSB5A5	.	sequence_feature	5864	5876	.	+	0	ID=BBa_B0043;Name=BBa_B0043
pSB5A5	.	sequence_feature	5864	5868	.	+	0	ID=annotation1797101;Name=Topoisomerase I recognition site;Parent=BBa_B0043
pSB5A5	.	sequence_feature	5868	5868	.	+	0	ID=annotation1797104;Name=Generated T overhang;Parent=BBa_B0043
pSB5A5	.	sequence_feature	5872	5876	.	+	0	ID=annotation1797102;Name=Nt.BstNB I recognition site;Parent=BBa_B0043
pSB5A5	.	sequence_feature	5869	5871	.	+	0	ID=annotation1797103;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0043
pSB5A5	.	terminator	5650	5721	.	+	0	ID=BBa_B0053;Name=BBa_B0053
pSB5A5	.	stem_loop	5658	5692	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
pSB5A5	.	sequence_feature	5844	5855	.	+	0	ID=BBa_B0042;Name=BBa_B0042
pSB5A5	.	stop_codon	5849	5851	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
pSB5A5	.	stop_codon	5844	5846	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
pSB5A5	.	stop_codon	5845	5847	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
pSB5A5	.	stop_codon	5853	5855	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
pSB5A5	.	stop_codon	5852	5854	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
pSB5A5	.	stop_codon	5848	5850	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
pSB5A5	.	sequence_feature	1	21	.	+	0	ID=BBa_G00001;Name=BBa_G00001
pSB5A5	.	engineered_region	1	21	.	+	0	ID=annotation1821544;Name=Suffix;Parent=BBa_G00001
pSB5A5	.	sequence_feature	16	21	.	+	0	ID=annotation1934227;Name=PstI;Parent=BBa_G00001
pSB5A5	.	sequence_feature	9	16	.	+	0	ID=annotation1934228;Name=NotI;Parent=BBa_G00001
pSB5A5	.	sequence_feature	2	7	.	+	0	ID=annotation1934226;Name=SpeI;Parent=BBa_G00001
pSB5A5	.	sequence_feature	148	167	.	+	0	ID=BBa_G00102;Name=BBa_G00102
pSB5A5	.	primer_binding_site	148	167	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
pSB5A5	.	sequence_feature	5885	5906	.	+	0	ID=BBa_G00000;Name=BBa_G00000
pSB5A5	.	engineered_region	5885	5906	.	+	0	ID=annotation1934225;Name=Prefix;Parent=BBa_G00000
pSB5A5	.	sequence_feature	5885	5890	.	+	0	ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000
pSB5A5	.	sequence_feature	5900	5905	.	+	0	ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000
pSB5A5	.	sequence_feature	5891	5898	.	+	0	ID=annotation1961228;Name=NotI site;Parent=BBa_G00000
pSB5A5	.	sequence_feature	1002	5641	.	+	0	ID=BBa_I50001;Name=BBa_I50001
pSB5A5	.	sequence_feature	1002	1704	.	+	0	ID=annotation1820911;Name=sopC (IncD) repeat region;Parent=BBa_I50001
pSB5A5	.	point_mutation	1931	1931	.	+	0	ID=annotation1820915;Name=A to T mutation (eliminates SpeI site);Parent=BBa_I50001
pSB5A5	.	CDS	4503	5258	.	+	0	ID=annotation1820922;Name=repE;Parent=BBa_I50001
pSB5A5	.	polyA_site	5495	5541	.	+	0	ID=annotation1820929;Name=A/T region;Parent=BBa_I50001
pSB5A5	.	sequence_feature	4252	5641	.	+	0	ID=annotation1820920;Name=sequence derived from F plasmid in GenBank Accession Number AP001918;Parent=BBa_I50001
pSB5A5	.	sequence_feature	1002	3922	.	+	0	ID=annotation1820919;Name=sequence derived from F plasmid in GenBank Accession Number AP001918;Parent=BBa_I50001
pSB5A5	.	operator	5545	5553	.	+	0	ID=annotation1820930;Name=DnaA box;Parent=BBa_I50001
pSB5A5	.	point_mutation	4629	4629	.	+	0	ID=annotation1820921;Name=C to T mutation (eliminates PstI site);Parent=BBa_I50001
pSB5A5	.	sequence_feature	5431	5450	.	+	0	ID=annotation1820926;Name=iteron (repeat region);Parent=BBa_I50001
pSB5A5	.	CDS	1778	2749	.	+	0	ID=annotation1820916;Name=parB;Parent=BBa_I50001
pSB5A5	.	point_mutation	3089	3089	.	+	0	ID=annotation1820917;Name=C to T mutation (eliminates PstI site);Parent=BBa_I50001
pSB5A5	.	sequence_feature	5410	5429	.	+	0	ID=annotation1820925;Name=iteron (repeat region);Parent=BBa_I50001
pSB5A5	.	sequence_alteration	5459	5459	.	+	0	ID=annotation1820927;Name=C to G mutation (eliminates XbaI site, noncoding region);Parent=BBa_I50001
pSB5A5	.	sequence_feature	5355	5374	.	+	0	ID=annotation1820923;Name=iteron (repeat region);Parent=BBa_I50001
pSB5A5	.	operator	5554	5561	.	+	0	ID=annotation1820931;Name=DnaA box;Parent=BBa_I50001
pSB5A5	.	sequence_feature	5377	5396	.	+	0	ID=annotation1820924;Name=iteron (repeat region);Parent=BBa_I50001
pSB5A5	.	sequence_feature	5352	5641	.	+	0	ID=annotation1820928;Name=ori2 (oriS) single copy replication origin;Parent=BBa_I50001
pSB5A5	.	CDS	2749	3924	.	+	0	ID=annotation1820918;Name=parA;Parent=BBa_I50001
>pSB5A5
tactagtagcggccgctgcagtactagaggagtcactaagggtactagagttagttagttagtactagagattagcaga
aagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattgggtactagaggctcactcaaa
ggcggtaattactagagcagataaaaaaaatccttagctttcgctaaggatgatttcttactagaggttgatcgggcac
gtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggag
ctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattt
tgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgta
aagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgta
tggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaac
gttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttac
ggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttca
ccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaagg
cgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaat
gaattacaacagtactgtgatgagtggcagggcggggcgtaataatactagagacttgcatcggatgcagcccggtgaa
cgtgccggcacggcctgggtaaccaggtattttgtccacataaccgtgcgcaaaatgttgtggataagcaggacacagc
agcaatccacagcaggcatacaaccgcacaccgaggttactccgttctacaggttacgacgacatgtcaatacttgccc
ttgacaggcattgatggaatcgtagtctcacgctgatagtctgatcgacaatacaagtgggaccgtggtcccagaccga
taatcagaccgacaacacgagtgggatcgtggtcccagactaataatcagaccgacgatacgagtgggaccgtggtccc
agactaataatcagaccgacgatacgagtgggaccgtggttccagactaataatcagaccgacgatacgagtgggaccg
tggtcccagactaataatcagaccgacgatacgagtgggaccatggtcccagactaataatcagaccgacgatacgagt
gggaccgtggtcccagtctgattatcagaccgacgatacgagtgggaccgtggtcccagactaataatcagaccgacga
tacgagtgggaccgtggtcccagactaataatcagaccgacgatacgagtgggaccgtggtcccagtctgattatcaga
ccgacgatacaagtggaacagtgggcccagagagaatattcaggccagttatgctttctggcctgtaacaaaggacatt
aagtaaagacagataaacgtagactaaaacgtggtcgcatcagggtgctggcttttcaagttccttaagaatggcctca
attttctctatacactcagttggaacacgagacctgtccaggttaagcaccattttatcgcccttatacaatactgtcg
ctccaggagcaaactgatgtcgtgagcttaaacttgttcttgatgcagatgacgttttaagcacagaagttaaaagagt
gataacttcttcagcttcaaatatcaccccagcttttttctgctcatgaaggttagatgcctgctgcttaagtaattcc
tctttatctgtaaaggctttttgaagtgcatcacctgaccgggcagatagttcaccggggtgagaaaaaagagcaacaa
ctgatttaggcaatttggcggtgttgatacagcgggtaataatcttacgtgaaatattttccgcatcagccagcgcaga
aatatttccagcaaattcattctgcaatcggcttgcataacgctgaccacgttcataagcacttgttgggcgataatcg
ttacccaatctggataatgcagccatctgctcatcatccagctcgccaaccagaacacgataatcactttcggtaagtg
cagcagctttacgacggcgactcccatcggcaatttctatgacaccagatactcttcgaccgaacgccggtgtctgttg
accagtcagtagaaaagaagggatgagatcatccagtgcgtcctcagtaagcagctcctggtcacgttcattacctgac
catacccgagaggtcttctcaacactatcaccccggagcacttcaagagtaaacttcacatcccgaccacatacaggca
aagtaatggcattaccgcgagccattactcctacgcgcgcaattaacgaatccaccatcggggcagctggtgtcgataa
cgaagtatcttcaaccggttgagtattgagcgtatgttttggaataacaggcgcacgcttcattatctaatctcccagc
gtggtttaatcagacgatcgaaaatttcattgcagacaggttcccaaatagaaagagcatttctccaggcaccagttga
agagcgttgatcaatggcctgttcaaaaacagttctcatccggatctgacctttaccaacttcatccgtttcacgtaca
acattttttagaaccatgcttccccaggcatcccgaatttgctcctccatccacggggactgagagccattactattgc
tgtatttggtaagcaaaatacgtacatcaggctcgaaccctttaagatcaacgttcttgagcagatcacgaagcatatc
gaaaaattgcagtgcggaggtgtagtcaaacaactcagcaggcgtgggaacaatcagcacatcagcagcacatacgaca
ttaatcgtgccgatacccaggttaggcgcgctgtcaataactatgacatcatagtcatgagcaacagtttcaatggcca
gtcggagcatcaggtgtggatcggtgggcagtttaccttcatcaaatttgcccattaactcagtttcaatacggtgcag
agccagacaggaaggaataatgtcaagccccggccagcaagtgggctttattgcataagtgacatcgtccttttcccca
agatagaaaggcaggagagtgtcttctgcatgaatatgaagatctggtacccatccgtgatacattgaggctgttccct
gggggtcgttaccttccacgagcaaaacacgtagccccttcagagccagatcctgagcaagatgaacagaaactgaggt
tttgtaaacgccacctttatgggcagcaaccccgatcaccggtggaaatacgtcttcagcacgtcgcaatcgcgtacca
aacacatcacgcatatgattaatttgttcaattgtataaccaacacgttgctcaacccgtcctcgaatttccatatccg
ggtgcggtagtcgccctgctttctcggcatctctgatagcctgagaagaaaccccaactaaatccgctgcttcacctat
tctccagcgccgggttattttcctcgcttccgggctgtcatcattaaactgtgcaatggcgatagccttcgtcatttca
tgaccagcgtttatgcactggttaagtgtttccatgagtttcattctgaacatcctttaatcattgctttgcgtttttt
tattaaatcttgcaatttactgcaaagcaacaacaaaatcgcaaagtcatcaaaaaaccgcaaagttgtttaaaataag
agcaacactacaaaaggagataagaagagcacatacctcagtcacttattatcactagcgctcgccgcagccgtgtaac
cgagcatagcgagcgaactggcgaggaagcaaagaagaactgttctgtcagatagctcttacgctcagcgcaagaagaa
atatccaccgtgggaaaaactccaggtagaggtacacacgcggatagccaattcagagtaataaactgtgataatcaac
cctcatcaatgatgacgaactaacccccgatatcaagtcacatgacgaagggaaagagaaggaaatcaactgtgacaaa
ctgccctcaaatttggcttccttaaaaattacagttcaaaaagtatgagaaaatccatgcaggctgaaggaaacagcaa
aactgtgacaaattaccctcagtaggtcagaacaaatgtgacgaaccaccctcaaatctgtgacagataaccctcagac
tatcctgtcgtcatggaagtgatatcgcggaaggaaaatacgatatgagtcgtctggcggcctttctttttctcaatgt
atgagaggcgcattggagttctgctgttgatctcattaacacagacttgcaggaagcggcggcggaagtcaggcatacg
ctggtaactttgaggcagctggtaacgctctatgatccagtcgattttcagagagacgatgcctgagccatccggctta
cgatactgacacagggattcgtataaacgcatggcatacggattggtgatttcttttgtttcactaagccgaaactgcg
taaaccggttctgtaacccgataaagaagggaatgagatatgggttgatatgtacactgtaaagccctctggatggact
gtgcgcacgtttgataaaccaaggaaaagattcatagcctttttcatcgccggcatcctcttcagggcgataaaaaacc
acttccttccccgcgaaactcttcaatgcctgccgtatatccttactggcttccgcagaggtcaatccgaatatttcag
catatttagcaacatggatctcgcagataccgtcatgttcctgtagggtgccatcagattttctgatctggtcaacgaa
cagatacagcatacgtttttgatcccgggagagactatatgccgcctcagtgaggtcgtttgactggacgattcgcggg
ctatttttacgtttcttgtgattgataaccgctgtttccgccatgacagatccatgtgaagtgtgacaagtttttagat
tgtcacactaaataaaaaagagtcaataagcagggataactttgtgaaaaaacagcttcttctgagggcaatttgtcac
agggttaagggcaatttgtcacagacaggactgtcatttgagggtgatttgtcacactgaaagggcaatttgtcacaac
accttctgtagaaccagcatggataaaggcctacaaggcgctctaaaaaagaagatctaaaaactataaaaaaaataat
tataaaaatatccccgtggataagtggataaccccaagggaagttttttcaggcatcgtgtgtaagcagaatatataag
tgctgttccctggtgcttcctcgctcactcgatactagagtccggcaaaaaaacgggcaaggtgtcaccaccctgccct
ttttctttaaaaccgaaaagattacttcgcgtttactagagtgccacctgacgtctaagaatactagagaaggaatatt
cagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaatactagagtta
gttagttagtactagagcccttagtgactctactagaggaattcgcggccgcttctagagtactagagggcttactaaa
agccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatg
tcaaaaagaggtatgctatgcagtttaaggtttacacctataaacgtgagagccgttatcgtctgtttgtggacgtaca
gagtgatattattgacacgcctgggcgtcgcatggtgattcccctggccagcgcacgtctgctttcagataaagtctcc
cgtgaactttacccggtggtgcatatcggggacgaaagctggcgcatgatgaccaccgatatggccagcgtgccggttt
ccgtgatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctgggg
aatataataatactagagcccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctact
tgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaac
tggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgta
gcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggt
tggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttgga
gcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcg
gacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatcttt
atagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaa
aaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat