LOCUS pSB6A1 4022 bp DNA linear 17-May-2024 DEFINITION pBR322 converted to BioBrick vector (formally BBa_I739201) ACCESSION pSB6A1 VERSION pSB6A1.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=BioBrick suffix CDS 23..918 /label=partial tetracycline resistance CDS (nonfunctional) misc_feature 1157..1161 /label=gamma-delta insertion sequence misc_feature 1278..1404 /label=from pSC101 CDS 1557..1748 /label=rop misc_feature 1653..1809 /label=H-strand Y effector site misc_feature 1993..2056 /label=L-strand Y effector site misc_feature 2176..2728 /label=RNAII transcript misc_feature 2161..2775 /label=rep pMB1 misc_feature 2618..2725 /label=RNAI transcript CDS 2935..3795 /label=beta-lactamase (bla gene) promoter 2582..2587 /label=RNAI transcript promoter -35 sequence promoter 2604..2609 /label=RNAI transcript promoter -10 sequence promoter 2738..2743 /label=RNAII transcript promoter -10 sequence promoter 2759..2764 /label=RNAII transcript promoter -35 sequence CDS 3727..3795 /label=beta-lactamase signal peptide CDS 2938..3726 /label=beta-lactamase mature peptide RBS 3803..3807 /label=bla gene promoter 3839..3844 /label=bla(P3) promoter -10 sequence promoter 3860..3865 /label=bla(P3) promoter -35 sequence misc_feature 2790..4003 /label=Repeat region 1 misc_feature 4001..4022 /label=BioBrick prefix ORIGIN 1 tactagtagc ggccgctgca gctctacgcc ggacgcatcg tggccggcat caccggcgcc 61 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc 121 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg 181 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc 241 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg 301 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc 361 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg 421 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg 481 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc 541 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac 601 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct 661 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac 721 catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga 781 ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg 841 attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc 901 cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa 961 gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga 1021 acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg 1081 ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc 1141 ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact 1201 gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt 1261 cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg 1321 caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt 1381 gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac 1441 aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc 1501 gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga 1561 ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc 1621 ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc 1681 acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa 1741 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 1801 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 1861 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat 1921 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 1981 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 2041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 2101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 2161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 2221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 2281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 2341 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 2401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 2461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 2521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 2581 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 2641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 2701 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 2761 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 2821 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 2881 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 2941 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 3001 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 3061 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 3121 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 3181 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 3241 tgccattgct gtaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 3301 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 3361 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 3421 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 3481 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 3541 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 3601 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 3661 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 3721 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 3781 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 3841 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 3901 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 3961 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattcgcgg ccgcttctag 4021 ag //