LOCUS       pSB6C5                  2356 bp    DNA     linear       25-Nov-2024
DEFINITION  pBR322 Derived Chloramphenicol Backbone
ACCESSION   pSB6C5
VERSION     pSB6C5.1  
FEATURES             Location/Qualifiers
     misc_feature    2335..2356
                     /label=Biobrick Prefix
     misc_feature    1..21
                     /label=Biobrick Suffix
     misc_feature    184..952
                     /label=P1004 (Cm Resistance)
     misc_feature    979..1172
                     /label=ROP
     misc_feature    1513..2127
                     /label=pMB1 Ori
     misc_feature    1479..1484
                     /label=Deleted Region / BamHI site
     primer_bind     116..135
                     /label=VR
     primer_bind     2212..2231
                     /label=VF2
ORIGIN
        1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc
       61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca
      121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta
      181 gaggttgatc gggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
      241 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      301 aatcacggga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      361 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      421 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      481 ccgcctgatg aacgctcacc cggagtttcg tatggccatg aaagacggtg agctggtgat
      541 ctgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcgtc
      601 cctctggagt gaataccacg acgatttccg gcagtttctc cacatatatt cgcaagatgt
      661 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
      721 tgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
      781 caacttcttc gcccccgttt tcacgatggg caaatattat acgcaaggcg acaaggtgct
      841 gatgccgctg gcgatccagg ttcatcatgc cgtttgtgat ggcttccatg tcggccgcat
      901 gcttaatgaa ttacaacagt actgtgatga gtggcagggc ggggcgtaat aatactagag
      961 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     1021 atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag
     1081 gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg
     1141 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     1201 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     1261 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     1321 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     1381 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     1441 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagg atccagcaaa aggccaggaa
     1501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     1981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2101 aaaaaaagga tctcaagaag atcctttgat cttactagct ccggcaaaaa aacgggcaag
     2161 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt ttgccacctg
     2221 acgtctaaga aaaggaatat tcagcaattt gcccgtgccg aagaaaggcc cacccgtgaa
     2281 ggtgagccag tgagttgatt gctacgtaat tagttagtta gcccttagtg actcgaattc
     2341 gcggccgctt ctagag
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