//plasmid/component/origin
type: Collection
persistent identity: plasmid component origin
display id: plasmid_component_origin
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: pSC101ts
member: p15A
member: Ori+Kan
member: F plasmid
member: BBa_I50030
member: BBa_K533000
member: pSC101
member: BBa_K112125
member: B-H-R plas
member: pSC101 ts
member: pUC19 ori
member: R6K
member: Ori
member: BBa_I50050
member: OriV
member: BBa_I50001
member: BBa_K864050
member: BBa_K524203

//plasmid/component
type: Collection
persistent identity: plasmid component
display id: plasmid_component
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //plasmid/component/other
member: //plasmid/component/origin
member: //plasmid/component/insulation
member: //plasmid/component/cloningsite
member: //plasmid/component/primerbindingsite

//plasmid/component/cloningsite
type: Collection
persistent identity: plasmid component cloningsite
display id: plasmid_component_cloningsite
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: Suffix
member: Prefix

//plasmid/component/insulation
type: Collection
persistent identity: plasmid component insulation
display id: plasmid_component_insulation
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_B0062
member: BBa_B0055
member: TSS
member: BBa_B0054
member: BBa_B0053

//plasmid/component/other
type: Collection
persistent identity: plasmid component other
display id: plasmid_component_other
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K863201
member: TOPO
member: NheI
member: GLF
member: Reporter +

//plasmid/component/primerbindingsite
type: Collection
persistent identity: plasmid component primerbindingsite
display id: plasmid_component_primerbindingsite
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: VR
member: Suffix
member: VF2
member: Prefix

Ori+Kan
type: ComponentDefinition
persistent identity: BBa K320004
display id: BBa_K320004
version: 1
was generated by: iGEM to SBOL conversion
description: Origin for Asaia and resistance to Kanamycin
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: t
owned by: james
owned by: myers
mutable description: t
discontinued: false
dominant: false
experience: experience/Issues
group u list: _439_
m user id: 0
owner id: 7149
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: t
star: false
creator: Perrudet Christian
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: KanR
sequence annotation: Asaia replication origin
sequence annotation: BBa_K320000
sequence: https://synbiohub.org/public/igem/BBa_K320004_sequence/1

KanR
type: SequenceAnnotation
persistent identity: annotation2091398
display id: annotation2091398
version: 1
top level: Ori+Kan
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2091398/range2091398/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2091398/range2091398/1
type: Range
persistent identity: range2091398
display id: range2091398
version: 1
top level: Ori+Kan
direction: direction/forward
start: 1400
end: 2372
orientation: inline

Asaia replication origin
type: SequenceAnnotation
persistent identity: annotation2091397
display id: annotation2091397
version: 1
top level: Ori+Kan
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2091397/range2091397/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2091397/range2091397/1
type: Range
persistent identity: range2091397
display id: range2091397
version: 1
top level: Ori+Kan
direction: direction/forward
start: 355
end: 1029
orientation: inline

BBa_K320000
type: SequenceAnnotation
persistent identity: annotation2088717
display id: annotation2088717
version: 1
top level: Ori+Kan
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2088717/range2088717/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2088717/range2088717/1
type: Range
persistent identity: range2088717
display id: range2088717
version: 1
top level: Ori+Kan
direction: direction/reverse
start: 1
end: 1399
orientation: inline

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

TSS
type: ComponentDefinition
persistent identity: BBa B0042
display id: BBa_B0042
version: 1
was generated by: iGEM to SBOL conversion
description: Translational stop sequence
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: stop codon (-1 frame)
sequence annotation: stop codon (+1 frame)
sequence annotation: stop codon (+3 frame)
sequence annotation: stop codon (-3 frame)
sequence annotation: stop codon (+2 frame)
sequence annotation: stop codon (-2 frame)
sequence: https://synbiohub.org/public/igem/BBa_B0042_sequence/1

stop codon (-1 frame)
type: SequenceAnnotation
persistent identity: annotation1797084
display id: annotation1797084
version: 1
top level: TSS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1

https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
type: Range
persistent identity: range1797084
display id: range1797084
version: 1
top level: TSS
direction: direction/reverse
start: 1
end: 3
orientation: inline

stop codon (+1 frame)
type: SequenceAnnotation
persistent identity: annotation1797086
display id: annotation1797086
version: 1
top level: TSS
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1

https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
type: Range
persistent identity: range1797086
display id: range1797086
version: 1
top level: TSS
direction: direction/forward
start: 10
end: 12
orientation: inline

stop codon (+3 frame)
type: SequenceAnnotation
persistent identity: annotation1797085
display id: annotation1797085
version: 1
top level: TSS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1

https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
type: Range
persistent identity: range1797085
display id: range1797085
version: 1
top level: TSS
direction: direction/forward
start: 6
end: 8
orientation: inline

stop codon (-3 frame)
type: SequenceAnnotation
persistent identity: annotation1797088
display id: annotation1797088
version: 1
top level: TSS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1

https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
type: Range
persistent identity: range1797088
display id: range1797088
version: 1
top level: TSS
direction: direction/reverse
start: 5
end: 7
orientation: inline

stop codon (+2 frame)
type: SequenceAnnotation
persistent identity: annotation1797083
display id: annotation1797083
version: 1
top level: TSS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1

https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
type: Range
persistent identity: range1797083
display id: range1797083
version: 1
top level: TSS
direction: direction/forward
start: 2
end: 4
orientation: inline

stop codon (-2 frame)
type: SequenceAnnotation
persistent identity: annotation1797087
display id: annotation1797087
version: 1
top level: TSS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1

https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
type: Range
persistent identity: range1797087
display id: range1797087
version: 1
top level: TSS
direction: direction/reverse
start: 9
end: 11
orientation: inline

BBa_I50001
type: ComponentDefinition
persistent identity: BBa I50001
display id: BBa_I50001
version: 1
was generated by: iGEM to SBOL conversion
description: F plasmid backbone with BioBrick sites removed, reverse
created: 2006-03-11T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: This is a (near) single copy origin derived from the F plasmid. The naturally occurring BioBricks sites were removed with an intent to preserve the amino acid and codon frequency where possible. Similar to the pSMART VC sequence from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. It is intended to be combined with other vector components (antibiotic resistance marker, multiple cloning site) in order to form a BioBricks vector. Relevant information can be found at http://openwetware.org/wiki/BioBrick_Parts_for_Plasmid_Engineering. This part is a reverse copy of BBa_I50000.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: iteron (repeat region)
sequence annotation: repE
sequence annotation: DnaA box
sequence annotation: sequence derived from F plasmid in GenBank Accession Number AP001918
sequence annotation: parB
sequence annotation: ori2 (oriS) single copy replication origin
sequence annotation: A/T region
sequence annotation: C to T mutation (eliminates PstI site)
sequence annotation: parA
sequence annotation: A to T mutation (eliminates SpeI site)
sequence annotation: sopC (IncD) repeat region
sequence annotation: C to G mutation (eliminates XbaI site, noncoding region)
sequence: https://synbiohub.org/public/igem/BBa_I50001_sequence/1

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1820926
display id: annotation1820926
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820926/range1820926/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820926/range1820926/1
type: Range
persistent identity: range1820926
display id: range1820926
version: 1
top level: BBa_I50001
direction: direction/reverse
start: 4430
end: 4449
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1820922
display id: annotation1820922
version: 1
top level: BBa_I50001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820922/range1820922/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820922/range1820922/1
type: Range
persistent identity: range1820922
display id: range1820922
version: 1
top level: BBa_I50001
direction: direction/reverse
start: 3502
end: 4257
orientation: inline

DnaA box
type: SequenceAnnotation
persistent identity: annotation1820931
display id: annotation1820931
version: 1
top level: BBa_I50001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820931/range1820931/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820931/range1820931/1
type: Range
persistent identity: range1820931
display id: range1820931
version: 1
top level: BBa_I50001
direction: direction/reverse
start: 4553
end: 4560
orientation: inline

sequence derived from F plasmid in GenBank Accession Number AP001918
type: SequenceAnnotation
persistent identity: annotation1820920
display id: annotation1820920
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820920/range1820920/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820920/range1820920/1
type: Range
persistent identity: range1820920
display id: range1820920
version: 1
top level: BBa_I50001
direction: direction/forward
start: 3251
end: 4640
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1820925
display id: annotation1820925
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820925/range1820925/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820925/range1820925/1
type: Range
persistent identity: range1820925
display id: range1820925
version: 1
top level: BBa_I50001
direction: direction/reverse
start: 4409
end: 4428
orientation: inline

parB
type: SequenceAnnotation
persistent identity: annotation1820916
display id: annotation1820916
version: 1
top level: BBa_I50001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820916/range1820916/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820916/range1820916/1
type: Range
persistent identity: range1820916
display id: range1820916
version: 1
top level: BBa_I50001
direction: direction/reverse
start: 777
end: 1748
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1820923
display id: annotation1820923
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820923/range1820923/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820923/range1820923/1
type: Range
persistent identity: range1820923
display id: range1820923
version: 1
top level: BBa_I50001
direction: direction/reverse
start: 4354
end: 4373
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1820924
display id: annotation1820924
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820924/range1820924/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820924/range1820924/1
type: Range
persistent identity: range1820924
display id: range1820924
version: 1
top level: BBa_I50001
direction: direction/reverse
start: 4376
end: 4395
orientation: inline

ori2 (oriS) single copy replication origin
type: SequenceAnnotation
persistent identity: annotation1820928
display id: annotation1820928
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820928/range1820928/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820928/range1820928/1
type: Range
persistent identity: range1820928
display id: range1820928
version: 1
top level: BBa_I50001
direction: direction/forward
start: 4351
end: 4640
orientation: inline

A/T region
type: SequenceAnnotation
persistent identity: annotation1820929
display id: annotation1820929
version: 1
top level: BBa_I50001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820929/range1820929/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820929/range1820929/1
type: Range
persistent identity: range1820929
display id: range1820929
version: 1
top level: BBa_I50001
direction: direction/reverse
start: 4494
end: 4540
orientation: inline

C to T mutation (eliminates PstI site)
type: SequenceAnnotation
persistent identity: annotation1820917
display id: annotation1820917
version: 1
top level: BBa_I50001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820917/range1820917/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820917/range1820917/1
type: Range
persistent identity: range1820917
display id: range1820917
version: 1
top level: BBa_I50001
direction: direction/forward
start: 2088
end: 2088
orientation: inline

sequence derived from F plasmid in GenBank Accession Number AP001918
type: SequenceAnnotation
persistent identity: annotation1820919
display id: annotation1820919
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820919/range1820919/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820919/range1820919/1
type: Range
persistent identity: range1820919
display id: range1820919
version: 1
top level: BBa_I50001
direction: direction/forward
start: 1
end: 2921
orientation: inline

DnaA box
type: SequenceAnnotation
persistent identity: annotation1820930
display id: annotation1820930
version: 1
top level: BBa_I50001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820930/range1820930/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820930/range1820930/1
type: Range
persistent identity: range1820930
display id: range1820930
version: 1
top level: BBa_I50001
direction: direction/reverse
start: 4544
end: 4552
orientation: inline

parA
type: SequenceAnnotation
persistent identity: annotation1820918
display id: annotation1820918
version: 1
top level: BBa_I50001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820918/range1820918/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820918/range1820918/1
type: Range
persistent identity: range1820918
display id: range1820918
version: 1
top level: BBa_I50001
direction: direction/reverse
start: 1748
end: 2923
orientation: inline

A to T mutation (eliminates SpeI site)
type: SequenceAnnotation
persistent identity: annotation1820915
display id: annotation1820915
version: 1
top level: BBa_I50001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820915/range1820915/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820915/range1820915/1
type: Range
persistent identity: range1820915
display id: range1820915
version: 1
top level: BBa_I50001
direction: direction/forward
start: 930
end: 930
orientation: inline

sopC (IncD) repeat region
type: SequenceAnnotation
persistent identity: annotation1820911
display id: annotation1820911
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820911/range1820911/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820911/range1820911/1
type: Range
persistent identity: range1820911
display id: range1820911
version: 1
top level: BBa_I50001
direction: direction/forward
start: 1
end: 703
orientation: inline

C to T mutation (eliminates PstI site)
type: SequenceAnnotation
persistent identity: annotation1820921
display id: annotation1820921
version: 1
top level: BBa_I50001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820921/range1820921/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820921/range1820921/1
type: Range
persistent identity: range1820921
display id: range1820921
version: 1
top level: BBa_I50001
direction: direction/forward
start: 3628
end: 3628
orientation: inline

C to G mutation (eliminates XbaI site, noncoding region)
type: SequenceAnnotation
persistent identity: annotation1820927
display id: annotation1820927
version: 1
top level: BBa_I50001
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820927/range1820927/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820927/range1820927/1
type: Range
persistent identity: range1820927
display id: range1820927
version: 1
top level: BBa_I50001
direction: direction/forward
start: 4458
end: 4458
orientation: inline

pSC101
type: ComponentDefinition
persistent identity: BBa I50042
display id: BBa_I50042
version: 1
was generated by: iGEM to SBOL conversion
description: pSC101 origin of replication
created: 2007-04-10T11:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: This part was amplified from pSB4A3-P1010.
owned by: james
owned by: myers
mutable description: pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The replication origin boundaries were chosen quite liberally (i.e. they might include more sequence than is strictly necessary) because a different version of this origin (BBa_I50040) was shown to be non-functional.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: promoter region
sequence annotation: T to C difference with canonical pSC101 vector sequence
sequence annotation: par region
sequence annotation: inverted repeat
sequence annotation: G to C difference with canonical pSC101 vector sequence
sequence annotation: -35
sequence annotation: mutated SpeI site
sequence annotation: repA
sequence annotation: RBS
sequence annotation: direct repeat
sequence annotation: C to T difference with canonical pSC101 vector sequence
sequence annotation: putative polyA
sequence annotation: -10
sequence annotation: Tn3 integration site
sequence annotation: AT rich region
sequence annotation: G to A difference with canonical pSC101 vector sequence
sequence annotation: Terminator
sequence: https://synbiohub.org/public/igem/BBa_I50042_sequence/1

promoter region
type: SequenceAnnotation
persistent identity: annotation2071323
display id: annotation2071323
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071323/range2071323/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071323/range2071323/1
type: Range
persistent identity: range2071323
display id: range2071323
version: 1
top level: pSC101
direction: direction/forward
start: 846
end: 865
orientation: inline

T to C difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071335
display id: annotation2071335
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071335/range2071335/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071335/range2071335/1
type: Range
persistent identity: range2071335
display id: range2071335
version: 1
top level: pSC101
direction: direction/forward
start: 2002
end: 2002
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation2071310
display id: annotation2071310
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071310/range2071310/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071310/range2071310/1
type: Range
persistent identity: range2071310
display id: range2071310
version: 1
top level: pSC101
direction: direction/forward
start: 137
end: 504
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071311
display id: annotation2071311
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071311/range2071311/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071311/range2071311/1
type: Range
persistent identity: range2071311
display id: range2071311
version: 1
top level: pSC101
direction: direction/forward
start: 139
end: 152
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071313
display id: annotation2071313
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071313/range2071313/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071313/range2071313/1
type: Range
persistent identity: range2071313
display id: range2071313
version: 1
top level: pSC101
direction: direction/forward
start: 165
end: 181
orientation: inline

G to C difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071332
display id: annotation2071332
version: 1
top level: pSC101
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071332/range2071332/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071332/range2071332/1
type: Range
persistent identity: range2071332
display id: range2071332
version: 1
top level: pSC101
direction: direction/forward
start: 1912
end: 1912
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2071321
display id: annotation2071321
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071321/range2071321/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071321/range2071321/1
type: Range
persistent identity: range2071321
display id: range2071321
version: 1
top level: pSC101
direction: direction/forward
start: 828
end: 833
orientation: inline

mutated SpeI site
type: SequenceAnnotation
persistent identity: annotation2071320
display id: annotation2071320
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071320/range2071320/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071320/range2071320/1
type: Range
persistent identity: range2071320
display id: range2071320
version: 1
top level: pSC101
direction: direction/reverse
start: 814
end: 819
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071315
display id: annotation2071315
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071315/range2071315/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071315/range2071315/1
type: Range
persistent identity: range2071315
display id: range2071315
version: 1
top level: pSC101
direction: direction/forward
start: 221
end: 238
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071334
display id: annotation2071334
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071334/range2071334/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071334/range2071334/1
type: Range
persistent identity: range2071334
display id: range2071334
version: 1
top level: pSC101
direction: direction/forward
start: 1963
end: 1979
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2071328
display id: annotation2071328
version: 1
top level: pSC101
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071328/range2071328/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071328/range2071328/1
type: Range
persistent identity: range2071328
display id: range2071328
version: 1
top level: pSC101
direction: direction/forward
start: 886
end: 1836
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071325
display id: annotation2071325
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071325/range2071325/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071325/range2071325/1
type: Range
persistent identity: range2071325
display id: range2071325
version: 1
top level: pSC101
direction: direction/forward
start: 862
end: 884
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071331
display id: annotation2071331
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071331/range2071331/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071331/range2071331/1
type: Range
persistent identity: range2071331
display id: range2071331
version: 1
top level: pSC101
direction: direction/forward
start: 1847
end: 1861
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071314
display id: annotation2071314
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071314/range2071314/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071314/range2071314/1
type: Range
persistent identity: range2071314
display id: range2071314
version: 1
top level: pSC101
direction: direction/forward
start: 192
end: 215
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2071327
display id: annotation2071327
version: 1
top level: pSC101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071327/range2071327/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071327/range2071327/1
type: Range
persistent identity: range2071327
display id: range2071327
version: 1
top level: pSC101
direction: direction/forward
start: 879
end: 884
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071322
display id: annotation2071322
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071322/range2071322/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071322/range2071322/1
type: Range
persistent identity: range2071322
display id: range2071322
version: 1
top level: pSC101
direction: direction/forward
start: 838
end: 859
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071318
display id: annotation2071318
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071318/range2071318/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071318/range2071318/1
type: Range
persistent identity: range2071318
display id: range2071318
version: 1
top level: pSC101
direction: direction/forward
start: 737
end: 756
orientation: inline

C to T difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071330
display id: annotation2071330
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071330/range2071330/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071330/range2071330/1
type: Range
persistent identity: range2071330
display id: range2071330
version: 1
top level: pSC101
direction: direction/forward
start: 1750
end: 1750
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071319
display id: annotation2071319
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071319/range2071319/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071319/range2071319/1
type: Range
persistent identity: range2071319
display id: range2071319
version: 1
top level: pSC101
direction: direction/forward
start: 769
end: 788
orientation: inline

putative polyA
type: SequenceAnnotation
persistent identity: annotation2071333
display id: annotation2071333
version: 1
top level: pSC101
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071333/range2071333/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071333/range2071333/1
type: Range
persistent identity: range2071333
display id: range2071333
version: 1
top level: pSC101
direction: direction/forward
start: 1947
end: 1962
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071317
display id: annotation2071317
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071317/range2071317/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071317/range2071317/1
type: Range
persistent identity: range2071317
display id: range2071317
version: 1
top level: pSC101
direction: direction/forward
start: 716
end: 735
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2071326
display id: annotation2071326
version: 1
top level: pSC101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071326/range2071326/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071326/range2071326/1
type: Range
persistent identity: range2071326
display id: range2071326
version: 1
top level: pSC101
direction: direction/forward
start: 870
end: 882
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2071324
display id: annotation2071324
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071324/range2071324/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071324/range2071324/1
type: Range
persistent identity: range2071324
display id: range2071324
version: 1
top level: pSC101
direction: direction/forward
start: 850
end: 855
orientation: inline

Tn3 integration site
type: SequenceAnnotation
persistent identity: annotation2071312
display id: annotation2071312
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071312/range2071312/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071312/range2071312/1
type: Range
persistent identity: range2071312
display id: range2071312
version: 1
top level: pSC101
direction: direction/forward
start: 158
end: 162
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation2071316
display id: annotation2071316
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071316/range2071316/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071316/range2071316/1
type: Range
persistent identity: range2071316
display id: range2071316
version: 1
top level: pSC101
direction: direction/forward
start: 635
end: 717
orientation: inline

G to A difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071329
display id: annotation2071329
version: 1
top level: pSC101
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071329/range2071329/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071329/range2071329/1
type: Range
persistent identity: range2071329
display id: range2071329
version: 1
top level: pSC101
direction: direction/forward
start: 1655
end: 1655
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2071309
display id: annotation2071309
version: 1
top level: pSC101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071309/range2071309/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071309/range2071309/1
type: Range
persistent identity: range2071309
display id: range2071309
version: 1
top level: pSC101
direction: direction/forward
start: 46
end: 75
orientation: inline

BBa_K533000
type: ComponentDefinition
persistent identity: BBa K533000
display id: BBa_K533000
version: 1
was generated by: iGEM to SBOL conversion
description: p15A replication origin
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
mutable provenance: Source: Cloned from plasmid pACYC184
owned by: james
owned by: myers
mutable description: p15A replication origin belongs to a different compatibility group from the common pMB1 (pBR or pUC) origin, and hence allows for co-existence with other pUC origin plasmids. When using this part, ligate it along with a proper antibiotic resistance gene and your gene of interest together to make a circular plasmid. Please do NOT include any other replication origin in your design.
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Unlike pMB1 origin, which allows for chloramphenicol amplification, p15A replication origin is insensitive to chloramphenicol and remains in low copy number. This replication origin is different from the original one in that it contains several mutations, which increases the copy number to be no lower than pBR origin. In this way, both the compatibility and the convenience for use are accommodated.
star: false
creator: ZHANG Yunxiao
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: p15A replication origin
sequence: https://synbiohub.org/public/igem/BBa_K533000_sequence/1

p15A replication origin
type: SequenceAnnotation
persistent identity: annotation2150403
display id: annotation2150403
version: 1
top level: BBa_K533000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K533000/annotation2150403/range2150403/1

https://synbiohub.org/public/igem/BBa_K533000/annotation2150403/range2150403/1
type: Range
persistent identity: range2150403
display id: range2150403
version: 1
top level: BBa_K533000
direction: direction/forward
start: 1
end: 914
orientation: inline

OriT-RP4
type: ComponentDefinition
persistent identity: BBa K125320
display id: BBa_K125320
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-RP4 (Origin of transfer for the RP4 plasmid)
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: RP4 NCBI accession number NC_001621
owned by: james
owned by: myers
mutable description: Relaxation region of self-transmissible plasmid RP4. When this part is added to a plasmid, it is transferred by RP4 through conjugation.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is similar to J01003, which has no experience and is much longer. In the process of designing and testing this part, it will be compared to J01003.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_K125320_sequence/1

Prefix
type: ComponentDefinition
persistent identity: BBa G00000
display id: BBa_G00000
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site prefix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks prefix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NotI site
sequence annotation: EcoRI site
sequence annotation: XbaI site
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_G00000_sequence/1

NotI site
type: SequenceAnnotation
persistent identity: annotation1961228
display id: annotation1961228
version: 1
top level: Prefix
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1

https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
type: Range
persistent identity: range1961228
display id: range1961228
version: 1
top level: Prefix
direction: direction/forward
start: 7
end: 14
orientation: inline

EcoRI site
type: SequenceAnnotation
persistent identity: annotation1934223
display id: annotation1934223
version: 1
top level: Prefix
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1

https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
type: Range
persistent identity: range1934223
display id: range1934223
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 6
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation1934224
display id: annotation1934224
version: 1
top level: Prefix
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1

https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
type: Range
persistent identity: range1934224
display id: range1934224
version: 1
top level: Prefix
direction: direction/forward
start: 16
end: 21
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1934225
display id: annotation1934225
version: 1
top level: Prefix
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1

https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
type: Range
persistent identity: range1934225
display id: range1934225
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 22
orientation: inline

GLF
type: ComponentDefinition
persistent identity: BBa K558005
display id: BBa_K558005
version: 1
was generated by: iGEM to SBOL conversion
description: Zymomonas mobilis glucose facilitator gene
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:41Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _726_
m user id: 0
owner id: 6570
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Vadim Gladwill, Laura Colgin
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: BBa_B0014 Double Terminator
sequence annotation: start
sequence annotation: GLF
sequence annotation: RBS
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K558005_sequence/1

BBa_B0014 Double Terminator
type: SequenceAnnotation
persistent identity: annotation2145002
display id: annotation2145002
version: 1
top level: GLF
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145002/range2145002/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145002/range2145002/1
type: Range
persistent identity: range2145002
display id: range2145002
version: 1
top level: GLF
direction: direction/forward
start: 1469
end: 1564
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2145030
display id: annotation2145030
version: 1
top level: GLF
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145030/range2145030/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145030/range2145030/1
type: Range
persistent identity: range2145030
display id: range2145030
version: 1
top level: GLF
direction: direction/forward
start: 18
end: 20
orientation: inline

GLF
type: SequenceAnnotation
persistent identity: annotation2145001
display id: annotation2145001
version: 1
top level: GLF
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145001/range2145001/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145001/range2145001/1
type: Range
persistent identity: range2145001
display id: range2145001
version: 1
top level: GLF
direction: direction/forward
start: 18
end: 1468
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2145000
display id: annotation2145000
version: 1
top level: GLF
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145000/range2145000/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145000/range2145000/1
type: Range
persistent identity: range2145000
display id: range2145000
version: 1
top level: GLF
direction: direction/forward
start: 1
end: 17
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2145031
display id: annotation2145031
version: 1
top level: GLF
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145031/range2145031/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145031/range2145031/1
type: Range
persistent identity: range2145031
display id: range2145031
version: 1
top level: GLF
direction: direction/forward
start: 1464
end: 1466
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2145032
display id: annotation2145032
version: 1
top level: GLF
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145032/range2145032/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145032/range2145032/1
type: Range
persistent identity: range2145032
display id: range2145032
version: 1
top level: GLF
direction: direction/forward
start: 1467
end: 1469
orientation: inline

NheI
type: ComponentDefinition
persistent identity: BBa B0045
display id: BBa_B0045
version: 1
was generated by: iGEM to SBOL conversion
description: NheI restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NheI site
sequence: https://synbiohub.org/public/igem/BBa_B0045_sequence/1

NheI site
type: SequenceAnnotation
persistent identity: annotation1822724
display id: annotation1822724
version: 1
top level: NheI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1

https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
type: Range
persistent identity: range1822724
display id: range1822724
version: 1
top level: NheI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K112125
type: ComponentDefinition
persistent identity: BBa K112125
display id: BBa_K112125
version: 1
was generated by: iGEM to SBOL conversion
description: OriV origin of replication
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:14Z
mutable provenance: cloned from E. coli genome
owned by: james
owned by: myers
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] <!-- Add more about the biology of this part here
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Christina Brown
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K112125_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p15A
type: ComponentDefinition
persistent identity: BBa I50032
display id: BBa_I50032
version: 1
was generated by: iGEM to SBOL conversion
description: p15A replication origin
created: 2007-04-10T11:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: This part was constructed by PCR amplification of pSB3K3-P1010.
owned by: james
owned by: myers
mutable description: p15A origin of replication which is supposed to have low to medium copy number. It is also the origin of replication in pSB3* series plasmids.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The boundaries of this part are quite liberal to ensure that this part is a functional origin.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: repeat region
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/igem/BBa_I50032_sequence/1

repeat region
type: SequenceAnnotation
persistent identity: annotation1928308
display id: annotation1928308
version: 1
top level: p15A
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50032/annotation1928308/range1928308/1

https://synbiohub.org/public/igem/BBa_I50032/annotation1928308/range1928308/1
type: Range
persistent identity: range1928308
display id: range1928308
version: 1
top level: p15A
direction: direction/forward
start: 1223
end: 1450
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation1928307
display id: annotation1928307
version: 1
top level: p15A
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50032/annotation1928307/range1928307/1

https://synbiohub.org/public/igem/BBa_I50032/annotation1928307/range1928307/1
type: Range
persistent identity: range1928307
display id: range1928307
version: 1
top level: p15A
direction: direction/forward
start: 399
end: 1223
orientation: inline

Rep
type: ComponentDefinition
persistent identity: BBa K125510
display id: BBa_K125510
version: 1
was generated by: iGEM to SBOL conversion
description: Replication Proteins
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.
owned by: james
owned by: myers
mutable description: Plasmids containing oriV and the corresponding Rep proteins (from RSF1010) are autonomously replicated in a broad range of hosts. It has been reported that RSF1010 derived plasmids containing oriV and the Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, Serratia (Leemans 1987), and several cyanobacteria strains (Mermet-Bouvier 1993) including Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part will be constructed with the origin of vegetative replication derived from an RSF1010 plasmid.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: I14032
sequence annotation: B0030
sequence annotation: RepB'
sequence annotation: Native Terminator
sequence annotation: Promoter[EFRepAC]
sequence annotation: [EFRepAC]
sequence: https://synbiohub.org/public/igem/BBa_K125510_sequence/1

I14032
type: SequenceAnnotation
persistent identity: annotation1974185
display id: annotation1974185
version: 1
top level: Rep
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974185/range1974185/1

https://synbiohub.org/public/igem/BBa_K125510/annotation1974185/range1974185/1
type: Range
persistent identity: range1974185
display id: range1974185
version: 1
top level: Rep
direction: direction/forward
start: 1
end: 37
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation1974186
display id: annotation1974186
version: 1
top level: Rep
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974186/range1974186/1

https://synbiohub.org/public/igem/BBa_K125510/annotation1974186/range1974186/1
type: Range
persistent identity: range1974186
display id: range1974186
version: 1
top level: Rep
direction: direction/forward
start: 46
end: 60
orientation: inline

RepB'
type: SequenceAnnotation
persistent identity: annotation1974187
display id: annotation1974187
version: 1
top level: Rep
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974187/range1974187/1

https://synbiohub.org/public/igem/BBa_K125510/annotation1974187/range1974187/1
type: Range
persistent identity: range1974187
display id: range1974187
version: 1
top level: Rep
direction: direction/forward
start: 67
end: 1038
orientation: inline

Native Terminator
type: SequenceAnnotation
persistent identity: annotation1974190
display id: annotation1974190
version: 1
top level: Rep
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974190/range1974190/1

https://synbiohub.org/public/igem/BBa_K125510/annotation1974190/range1974190/1
type: Range
persistent identity: range1974190
display id: range1974190
version: 1
top level: Rep
direction: direction/forward
start: 3353
end: 3385
orientation: inline

Promoter[EFRepAC]
type: SequenceAnnotation
persistent identity: annotation1974188
display id: annotation1974188
version: 1
top level: Rep
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974188/range1974188/1

https://synbiohub.org/public/igem/BBa_K125510/annotation1974188/range1974188/1
type: Range
persistent identity: range1974188
display id: range1974188
version: 1
top level: Rep
direction: direction/forward
start: 1028
end: 1079
orientation: inline

[EFRepAC]
type: SequenceAnnotation
persistent identity: annotation1974189
display id: annotation1974189
version: 1
top level: Rep
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974189/range1974189/1

https://synbiohub.org/public/igem/BBa_K125510/annotation1974189/range1974189/1
type: Range
persistent identity: range1974189
display id: range1974189
version: 1
top level: Rep
direction: direction/forward
start: 1099
end: 3226
orientation: inline

F plasmid
type: ComponentDefinition
persistent identity: BBa I50000
display id: BBa_I50000
version: 1
was generated by: iGEM to SBOL conversion
description: F plasmid backbone with BioBrick sites removed.
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: This is a (near) single copy origin derived from the F plasmid. The naturally occurring BioBricks sites were removed with an intent to preserve the amino acid and codon frequency where possible. Similar to the pSMART VC sequence from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. It is intended to be combined with other vector components (antibiotic resistance marker, multiple cloning site) in order to form a BioBricks vector. Relevant information can be found at http://openwetware.org/wiki/BioBrick_Parts_for_Plasmid_Engineering.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: sopC(IncD) repeat region
sequence annotation: A/T region
sequence annotation: parB
sequence annotation: repE
sequence annotation: DnaA box
sequence annotation: A to T mutation (eliminates SpeI site)
sequence annotation: C to T mutation (eliminates PstI site)
sequence annotation: iteron (repeat region)
sequence annotation: ori2 (oriS) single copy replication origin
sequence annotation: parA
sequence annotation: sequence derived from F plasmid in GenBank Accession Number AP001918
sequence annotation: C to G mutation (eliminates XbaI site, noncoding region)
sequence: https://synbiohub.org/public/igem/BBa_I50000_sequence/1

sopC(IncD) repeat region
type: SequenceAnnotation
persistent identity: annotation1757395
display id: annotation1757395
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757395/range1757395/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757395/range1757395/1
type: Range
persistent identity: range1757395
display id: range1757395
version: 1
top level: F plasmid
direction: direction/reverse
start: 3938
end: 4640
orientation: inline

A/T region
type: SequenceAnnotation
persistent identity: annotation1757415
display id: annotation1757415
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757415/range1757415/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757415/range1757415/1
type: Range
persistent identity: range1757415
display id: range1757415
version: 1
top level: F plasmid
direction: direction/forward
start: 101
end: 147
orientation: inline

parB
type: SequenceAnnotation
persistent identity: annotation1757405
display id: annotation1757405
version: 1
top level: F plasmid
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757405/range1757405/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757405/range1757405/1
type: Range
persistent identity: range1757405
display id: range1757405
version: 1
top level: F plasmid
direction: direction/forward
start: 2893
end: 3864
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1757408
display id: annotation1757408
version: 1
top level: F plasmid
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757408/range1757408/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757408/range1757408/1
type: Range
persistent identity: range1757408
display id: range1757408
version: 1
top level: F plasmid
direction: direction/forward
start: 384
end: 1139
orientation: inline

DnaA box
type: SequenceAnnotation
persistent identity: annotation1757414
display id: annotation1757414
version: 1
top level: F plasmid
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757414/range1757414/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757414/range1757414/1
type: Range
persistent identity: range1757414
display id: range1757414
version: 1
top level: F plasmid
direction: direction/forward
start: 89
end: 97
orientation: inline

A to T mutation (eliminates SpeI site)
type: SequenceAnnotation
persistent identity: annotation1757411
display id: annotation1757411
version: 1
top level: F plasmid
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757411/range1757411/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757411/range1757411/1
type: Range
persistent identity: range1757411
display id: range1757411
version: 1
top level: F plasmid
direction: direction/reverse
start: 3711
end: 3711
orientation: inline

C to T mutation (eliminates PstI site)
type: SequenceAnnotation
persistent identity: annotation1757410
display id: annotation1757410
version: 1
top level: F plasmid
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757410/range1757410/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757410/range1757410/1
type: Range
persistent identity: range1757410
display id: range1757410
version: 1
top level: F plasmid
direction: direction/reverse
start: 2553
end: 2553
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757416
display id: annotation1757416
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757416/range1757416/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757416/range1757416/1
type: Range
persistent identity: range1757416
display id: range1757416
version: 1
top level: F plasmid
direction: direction/forward
start: 192
end: 211
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757417
display id: annotation1757417
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757417/range1757417/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757417/range1757417/1
type: Range
persistent identity: range1757417
display id: range1757417
version: 1
top level: F plasmid
direction: direction/forward
start: 213
end: 232
orientation: inline

ori2 (oriS) single copy replication origin
type: SequenceAnnotation
persistent identity: annotation1757409
display id: annotation1757409
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757409/range1757409/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757409/range1757409/1
type: Range
persistent identity: range1757409
display id: range1757409
version: 1
top level: F plasmid
direction: direction/reverse
start: 1
end: 290
orientation: inline

parA
type: SequenceAnnotation
persistent identity: annotation1757406
display id: annotation1757406
version: 1
top level: F plasmid
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757406/range1757406/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757406/range1757406/1
type: Range
persistent identity: range1757406
display id: range1757406
version: 1
top level: F plasmid
direction: direction/forward
start: 1718
end: 2893
orientation: inline

sequence derived from F plasmid in GenBank Accession Number AP001918
type: SequenceAnnotation
persistent identity: annotation1757421
display id: annotation1757421
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757421/range1757421/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757421/range1757421/1
type: Range
persistent identity: range1757421
display id: range1757421
version: 1
top level: F plasmid
direction: direction/reverse
start: 1720
end: 4640
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757418
display id: annotation1757418
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757418/range1757418/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757418/range1757418/1
type: Range
persistent identity: range1757418
display id: range1757418
version: 1
top level: F plasmid
direction: direction/forward
start: 246
end: 265
orientation: inline

sequence derived from F plasmid in GenBank Accession Number AP001918
type: SequenceAnnotation
persistent identity: annotation1757407
display id: annotation1757407
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757407/range1757407/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757407/range1757407/1
type: Range
persistent identity: range1757407
display id: range1757407
version: 1
top level: F plasmid
direction: direction/reverse
start: 1
end: 1390
orientation: inline

C to T mutation (eliminates PstI site)
type: SequenceAnnotation
persistent identity: annotation1757412
display id: annotation1757412
version: 1
top level: F plasmid
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757412/range1757412/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757412/range1757412/1
type: Range
persistent identity: range1757412
display id: range1757412
version: 1
top level: F plasmid
direction: direction/reverse
start: 1013
end: 1013
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757419
display id: annotation1757419
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757419/range1757419/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757419/range1757419/1
type: Range
persistent identity: range1757419
display id: range1757419
version: 1
top level: F plasmid
direction: direction/forward
start: 268
end: 287
orientation: inline

C to G mutation (eliminates XbaI site, noncoding region)
type: SequenceAnnotation
persistent identity: annotation1757413
display id: annotation1757413
version: 1
top level: F plasmid
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757413/range1757413/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757413/range1757413/1
type: Range
persistent identity: range1757413
display id: range1757413
version: 1
top level: F plasmid
direction: direction/reverse
start: 183
end: 183
orientation: inline

DnaA box
type: SequenceAnnotation
persistent identity: annotation1757420
display id: annotation1757420
version: 1
top level: F plasmid
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757420/range1757420/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757420/range1757420/1
type: Range
persistent identity: range1757420
display id: range1757420
version: 1
top level: F plasmid
direction: direction/forward
start: 81
end: 88
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: iGEM to SBOL conversion
description: promoter P(Lac) IQ
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, High Transcription
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: P(Lac) IQ
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 26
end: 31
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 1
end: 37
orientation: inline

OriV
type: ComponentDefinition
persistent identity: BBa K125340
display id: BBa_K125340
version: 1
was generated by: iGEM to SBOL conversion
description: Origin of Vegetative Replication
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.
owned by: james
owned by: myers
mutable description: Plasmids containing oriV and the corresponding Rep proteins (from RSF1010) are autonomously replicated in a broad range of hosts. It has been reported that RSF1010 derived plasmids containing oriV and the Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, Serratia (Leemans 1987), and several cyanobacteria strains (Mermet-Bouvier 1993) including Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is to be constructed with the Rep proteins derived from an RSF1010 plasmid.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RepB Binding Site
sequence annotation: RepA Binding Site
sequence annotation: RepC Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K125340_sequence/1

RepB Binding Site
type: SequenceAnnotation
persistent identity: annotation1974215
display id: annotation1974215
version: 1
top level: OriV
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974215/range1974215/1

https://synbiohub.org/public/igem/BBa_K125340/annotation1974215/range1974215/1
type: Range
persistent identity: range1974215
display id: range1974215
version: 1
top level: OriV
direction: direction/forward
start: 207
end: 352
orientation: inline

RepA Binding Site
type: SequenceAnnotation
persistent identity: annotation1974216
display id: annotation1974216
version: 1
top level: OriV
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974216/range1974216/1

https://synbiohub.org/public/igem/BBa_K125340/annotation1974216/range1974216/1
type: Range
persistent identity: range1974216
display id: range1974216
version: 1
top level: OriV
direction: direction/forward
start: 353
end: 401
orientation: inline

RepC Binding Site
type: SequenceAnnotation
persistent identity: annotation1974214
display id: annotation1974214
version: 1
top level: OriV
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974214/range1974214/1

https://synbiohub.org/public/igem/BBa_K125340/annotation1974214/range1974214/1
type: Range
persistent identity: range1974214
display id: range1974214
version: 1
top level: OriV
direction: direction/forward
start: 3
end: 66
orientation: inline

BBa_K125500
type: ComponentDefinition
persistent identity: BBa K125500
display id: BBa_K125500
version: 1
was generated by: iGEM to SBOL conversion
description: GFP fusion brick
created: 2008-07-24T11:00:00Z
modified: 2016-01-25T02:34:56Z
mutable provenance: The GFP fusion brick was derived from the GFP BioBrick, [http://partsregistry.org/Part:BBa_E0040 BBa_E0040].
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 4206
owner id: 3229
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The first two nucleotides of the GFP start codon were removed to create this fusion brick.
star: false
creator: Grace Kwan
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP fusion brick
sequence annotation: deletion of TG
sequence: https://synbiohub.org/public/igem/BBa_K125500_sequence/1

GFP fusion brick
type: SequenceAnnotation
persistent identity: annotation1968260
display id: annotation1968260
version: 1
top level: BBa_K125500
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1

https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1
type: Range
persistent identity: range1968260
display id: range1968260
version: 1
top level: BBa_K125500
direction: direction/forward
start: 1
end: 718
orientation: inline

deletion of TG
type: SequenceAnnotation
persistent identity: annotation1969908
display id: annotation1969908
version: 1
top level: BBa_K125500
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1

https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1
type: Range
persistent identity: range1969908
display id: range1969908
version: 1
top level: BBa_K125500
direction: direction/forward
start: 1
end: 1
orientation: inline

slr2016 SS
type: ComponentDefinition
persistent identity: BBa K125310
display id: BBa_K125310
version: 1
was generated by: iGEM to SBOL conversion
description: slr2016 signal sequence from cyanobacterium Synechocystis; secretes protein
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: The ''slr2016'' signal sequence is located at the amino terminus of slr2016 polypeptides synthesized by ''Synechocystis'' sp. PCC 6803.
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3229
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Krystle Salazar and Grace Kwan
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: slr2016 signal sequence
sequence: https://synbiohub.org/public/igem/BBa_K125310_sequence/1

slr2016 signal sequence
type: SequenceAnnotation
persistent identity: annotation1968259
display id: annotation1968259
version: 1
top level: slr2016 SS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125310/annotation1968259/range1968259/1

https://synbiohub.org/public/igem/BBa_K125310/annotation1968259/range1968259/1
type: Range
persistent identity: range1968259
display id: range1968259
version: 1
top level: slr2016 SS
direction: direction/forward
start: 1
end: 53
orientation: inline

pSC101 ts
type: ComponentDefinition
persistent identity: BBa I50052
display id: BBa_I50052
version: 1
was generated by: iGEM to SBOL conversion
description: pSC101 temperature sensitive origin of replication
created: 2010-06-20T11:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: This part is derived from BBa_I50042. It contains a point mutation that changes Alanine to Valine in RepA protein.
owned by: james
owned by: myers
mutable description: BBa_I50052 is a pSC101 temperature sensitive origin of replication. BBa_I50052 is the temperature sensitive replication origin found in the pSB8* series of BioBrick vectors as well as the pSIM5 recombineering vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: See design notes for Part:BBa_I50042.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: G to A difference with canonical pSC101 vector sequence
sequence annotation: repA
sequence annotation: -35
sequence annotation: G to C difference with canonical pSC101 vector sequence
sequence annotation: putative polyA
sequence annotation: promoter region
sequence annotation: inverted repeat
sequence annotation: RBS
sequence annotation: mutated SpeI site
sequence annotation: T to C difference with canonical pSC101 vector sequence
sequence annotation: -10
sequence annotation: AT rich region
sequence annotation: direct repeat
sequence annotation: Terminator
sequence annotation: C to T difference with canonical pSC101 vector sequence
sequence annotation: par region
sequence annotation: Tn3 integration site
sequence annotation: GCT->GTT ts mutation (Ala->Val)
sequence: https://synbiohub.org/public/igem/BBa_I50052_sequence/1

G to A difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071358
display id: annotation2071358
version: 1
top level: pSC101 ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071358/range2071358/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071358/range2071358/1
type: Range
persistent identity: range2071358
display id: range2071358
version: 1
top level: pSC101 ts
direction: direction/forward
start: 1655
end: 1655
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2071355
display id: annotation2071355
version: 1
top level: pSC101 ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071355/range2071355/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071355/range2071355/1
type: Range
persistent identity: range2071355
display id: range2071355
version: 1
top level: pSC101 ts
direction: direction/forward
start: 886
end: 1836
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2071348
display id: annotation2071348
version: 1
top level: pSC101 ts
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071348/range2071348/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071348/range2071348/1
type: Range
persistent identity: range2071348
display id: range2071348
version: 1
top level: pSC101 ts
direction: direction/forward
start: 828
end: 833
orientation: inline

G to C difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071361
display id: annotation2071361
version: 1
top level: pSC101 ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071361/range2071361/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071361/range2071361/1
type: Range
persistent identity: range2071361
display id: range2071361
version: 1
top level: pSC101 ts
direction: direction/forward
start: 1912
end: 1912
orientation: inline

putative polyA
type: SequenceAnnotation
persistent identity: annotation2071359
display id: annotation2071359
version: 1
top level: pSC101 ts
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071359/range2071359/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071359/range2071359/1
type: Range
persistent identity: range2071359
display id: range2071359
version: 1
top level: pSC101 ts
direction: direction/forward
start: 1947
end: 1962
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation2071350
display id: annotation2071350
version: 1
top level: pSC101 ts
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071350/range2071350/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071350/range2071350/1
type: Range
persistent identity: range2071350
display id: range2071350
version: 1
top level: pSC101 ts
direction: direction/forward
start: 846
end: 865
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071356
display id: annotation2071356
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071356/range2071356/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071356/range2071356/1
type: Range
persistent identity: range2071356
display id: range2071356
version: 1
top level: pSC101 ts
direction: direction/forward
start: 1847
end: 1861
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071340
display id: annotation2071340
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071340/range2071340/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071340/range2071340/1
type: Range
persistent identity: range2071340
display id: range2071340
version: 1
top level: pSC101 ts
direction: direction/forward
start: 165
end: 181
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071349
display id: annotation2071349
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071349/range2071349/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071349/range2071349/1
type: Range
persistent identity: range2071349
display id: range2071349
version: 1
top level: pSC101 ts
direction: direction/forward
start: 838
end: 859
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2071353
display id: annotation2071353
version: 1
top level: pSC101 ts
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071353/range2071353/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071353/range2071353/1
type: Range
persistent identity: range2071353
display id: range2071353
version: 1
top level: pSC101 ts
direction: direction/forward
start: 870
end: 882
orientation: inline

mutated SpeI site
type: SequenceAnnotation
persistent identity: annotation2071347
display id: annotation2071347
version: 1
top level: pSC101 ts
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071347/range2071347/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071347/range2071347/1
type: Range
persistent identity: range2071347
display id: range2071347
version: 1
top level: pSC101 ts
direction: direction/forward
start: 814
end: 819
orientation: inline

T to C difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071362
display id: annotation2071362
version: 1
top level: pSC101 ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071362/range2071362/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071362/range2071362/1
type: Range
persistent identity: range2071362
display id: range2071362
version: 1
top level: pSC101 ts
direction: direction/forward
start: 2002
end: 2002
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2071351
display id: annotation2071351
version: 1
top level: pSC101 ts
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071351/range2071351/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071351/range2071351/1
type: Range
persistent identity: range2071351
display id: range2071351
version: 1
top level: pSC101 ts
direction: direction/forward
start: 850
end: 855
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation2071343
display id: annotation2071343
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071343/range2071343/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071343/range2071343/1
type: Range
persistent identity: range2071343
display id: range2071343
version: 1
top level: pSC101 ts
direction: direction/forward
start: 635
end: 717
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071342
display id: annotation2071342
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071342/range2071342/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071342/range2071342/1
type: Range
persistent identity: range2071342
display id: range2071342
version: 1
top level: pSC101 ts
direction: direction/forward
start: 221
end: 238
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071352
display id: annotation2071352
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071352/range2071352/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071352/range2071352/1
type: Range
persistent identity: range2071352
display id: range2071352
version: 1
top level: pSC101 ts
direction: direction/forward
start: 862
end: 884
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071345
display id: annotation2071345
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071345/range2071345/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071345/range2071345/1
type: Range
persistent identity: range2071345
display id: range2071345
version: 1
top level: pSC101 ts
direction: direction/forward
start: 737
end: 756
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071344
display id: annotation2071344
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071344/range2071344/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071344/range2071344/1
type: Range
persistent identity: range2071344
display id: range2071344
version: 1
top level: pSC101 ts
direction: direction/forward
start: 716
end: 735
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2071336
display id: annotation2071336
version: 1
top level: pSC101 ts
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071336/range2071336/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071336/range2071336/1
type: Range
persistent identity: range2071336
display id: range2071336
version: 1
top level: pSC101 ts
direction: direction/forward
start: 46
end: 75
orientation: inline

C to T difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071360
display id: annotation2071360
version: 1
top level: pSC101 ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071360/range2071360/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071360/range2071360/1
type: Range
persistent identity: range2071360
display id: range2071360
version: 1
top level: pSC101 ts
direction: direction/forward
start: 1750
end: 1750
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation2071337
display id: annotation2071337
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071337/range2071337/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071337/range2071337/1
type: Range
persistent identity: range2071337
display id: range2071337
version: 1
top level: pSC101 ts
direction: direction/forward
start: 137
end: 504
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071341
display id: annotation2071341
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071341/range2071341/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071341/range2071341/1
type: Range
persistent identity: range2071341
display id: range2071341
version: 1
top level: pSC101 ts
direction: direction/forward
start: 192
end: 215
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071346
display id: annotation2071346
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071346/range2071346/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071346/range2071346/1
type: Range
persistent identity: range2071346
display id: range2071346
version: 1
top level: pSC101 ts
direction: direction/forward
start: 769
end: 788
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2071354
display id: annotation2071354
version: 1
top level: pSC101 ts
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071354/range2071354/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071354/range2071354/1
type: Range
persistent identity: range2071354
display id: range2071354
version: 1
top level: pSC101 ts
direction: direction/forward
start: 879
end: 884
orientation: inline

Tn3 integration site
type: SequenceAnnotation
persistent identity: annotation2071339
display id: annotation2071339
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071339/range2071339/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071339/range2071339/1
type: Range
persistent identity: range2071339
display id: range2071339
version: 1
top level: pSC101 ts
direction: direction/forward
start: 158
end: 162
orientation: inline

GCT->GTT ts mutation (Ala->Val)
type: SequenceAnnotation
persistent identity: annotation2071357
display id: annotation2071357
version: 1
top level: pSC101 ts
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071357/range2071357/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071357/range2071357/1
type: Range
persistent identity: range2071357
display id: range2071357
version: 1
top level: pSC101 ts
direction: direction/forward
start: 1051
end: 1053
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071338
display id: annotation2071338
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071338/range2071338/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071338/range2071338/1
type: Range
persistent identity: range2071338
display id: range2071338
version: 1
top level: pSC101 ts
direction: direction/forward
start: 139
end: 152
orientation: inline

BBa_I50030
type: ComponentDefinition
persistent identity: BBa I50030
display id: BBa_I50030
version: 1
was generated by: iGEM to SBOL conversion
description: pBR322 replication origin
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:42Z
owned by: james
owned by: myers
mutable description: This part is the replication origin from plasmid pBR322 which is supposed to have low to medium copy number.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: replication origin
sequence annotation: H-strand Y effector site
sequence annotation: RNAI transcript
sequence annotation: from pSC101
sequence annotation: RBS 1
sequence annotation: repeat region found in Tn3
sequence annotation: rep pMB1
sequence annotation: RNAII transcript
sequence annotation: RNAI transcript promoter -35 sequence
sequence annotation: L-strand Y effector site
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: gamma-delta insertion target sequence
sequence annotation: RNAI transcript promoter -10 sequence
sequence annotation: pMB1 origin of replication
sequence annotation: RBS 2
sequence: https://synbiohub.org/public/igem/BBa_I50030_sequence/1

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1796196
display id: annotation1796196
version: 1
top level: BBa_I50030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796196/range1796196/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796196/range1796196/1
type: Range
persistent identity: range1796196
display id: range1796196
version: 1
top level: BBa_I50030
direction: direction/forward
start: 171
end: 176
orientation: inline

replication origin
type: SequenceAnnotation
persistent identity: annotation1796203
display id: annotation1796203
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796203/range1796203/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796203/range1796203/1
type: Range
persistent identity: range1796203
display id: range1796203
version: 1
top level: BBa_I50030
direction: direction/reverse
start: 758
end: 758
orientation: inline

H-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1796206
display id: annotation1796206
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796206/range1796206/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796206/range1796206/1
type: Range
persistent identity: range1796206
display id: range1796206
version: 1
top level: BBa_I50030
direction: direction/reverse
start: 1126
end: 1282
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1796198
display id: annotation1796198
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796198/range1796198/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796198/range1796198/1
type: Range
persistent identity: range1796198
display id: range1796198
version: 1
top level: BBa_I50030
direction: direction/reverse
start: 210
end: 317
orientation: inline

from pSC101
type: SequenceAnnotation
persistent identity: annotation1796210
display id: annotation1796210
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796210/range1796210/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796210/range1796210/1
type: Range
persistent identity: range1796210
display id: range1796210
version: 1
top level: BBa_I50030
direction: direction/reverse
start: 1531
end: 1657
orientation: inline

RBS 1
type: SequenceAnnotation
persistent identity: annotation1796207
display id: annotation1796207
version: 1
top level: BBa_I50030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796207/range1796207/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796207/range1796207/1
type: Range
persistent identity: range1796207
display id: range1796207
version: 1
top level: BBa_I50030
direction: direction/reverse
start: 1383
end: 1388
orientation: inline

repeat region found in Tn3
type: SequenceAnnotation
persistent identity: annotation1796195
display id: annotation1796195
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796195/range1796195/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796195/range1796195/1
type: Range
persistent identity: range1796195
display id: range1796195
version: 1
top level: BBa_I50030
direction: direction/reverse
start: 108
end: 145
orientation: inline

rep pMB1
type: SequenceAnnotation
persistent identity: annotation1796201
display id: annotation1796201
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796201/range1796201/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796201/range1796201/1
type: Range
persistent identity: range1796201
display id: range1796201
version: 1
top level: BBa_I50030
direction: direction/forward
start: 160
end: 774
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1796202
display id: annotation1796202
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796202/range1796202/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796202/range1796202/1
type: Range
persistent identity: range1796202
display id: range1796202
version: 1
top level: BBa_I50030
direction: direction/forward
start: 207
end: 759
orientation: inline

RNAI transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1796200
display id: annotation1796200
version: 1
top level: BBa_I50030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796200/range1796200/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796200/range1796200/1
type: Range
persistent identity: range1796200
display id: range1796200
version: 1
top level: BBa_I50030
direction: direction/reverse
start: 348
end: 353
orientation: inline

L-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1796204
display id: annotation1796204
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796204/range1796204/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796204/range1796204/1
type: Range
persistent identity: range1796204
display id: range1796204
version: 1
top level: BBa_I50030
direction: direction/forward
start: 879
end: 942
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1796197
display id: annotation1796197
version: 1
top level: BBa_I50030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796197/range1796197/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796197/range1796197/1
type: Range
persistent identity: range1796197
display id: range1796197
version: 1
top level: BBa_I50030
direction: direction/forward
start: 192
end: 197
orientation: inline

gamma-delta insertion target sequence
type: SequenceAnnotation
persistent identity: annotation1796211
display id: annotation1796211
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796211/range1796211/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796211/range1796211/1
type: Range
persistent identity: range1796211
display id: range1796211
version: 1
top level: BBa_I50030
direction: direction/forward
start: 1774
end: 1778
orientation: inline

gamma-delta insertion target sequence
type: SequenceAnnotation
persistent identity: annotation1796209
display id: annotation1796209
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796209/range1796209/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796209/range1796209/1
type: Range
persistent identity: range1796209
display id: range1796209
version: 1
top level: BBa_I50030
direction: direction/forward
start: 1501
end: 1505
orientation: inline

gamma-delta insertion target sequence
type: SequenceAnnotation
persistent identity: annotation1796205
display id: annotation1796205
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796205/range1796205/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796205/range1796205/1
type: Range
persistent identity: range1796205
display id: range1796205
version: 1
top level: BBa_I50030
direction: direction/forward
start: 1044
end: 1048
orientation: inline

RNAI transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1796199
display id: annotation1796199
version: 1
top level: BBa_I50030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796199/range1796199/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796199/range1796199/1
type: Range
persistent identity: range1796199
display id: range1796199
version: 1
top level: BBa_I50030
direction: direction/reverse
start: 326
end: 331
orientation: inline

pMB1 origin of replication
type: SequenceAnnotation
persistent identity: annotation1796194
display id: annotation1796194
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796194/range1796194/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796194/range1796194/1
type: Range
persistent identity: range1796194
display id: range1796194
version: 1
top level: BBa_I50030
direction: direction/forward
start: 171
end: 759
orientation: inline

RBS 2
type: SequenceAnnotation
persistent identity: annotation1796208
display id: annotation1796208
version: 1
top level: BBa_I50030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796208/range1796208/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796208/range1796208/1
type: Range
persistent identity: range1796208
display id: range1796208
version: 1
top level: BBa_I50030
direction: direction/reverse
start: 1384
end: 1388
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0012

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0052)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
top level: BBa_B0062
direction: direction/forward
start: 14
end: 33
orientation: inline

BBa_K524203
type: ComponentDefinition
persistent identity: BBa K524203
display id: BBa_K524203
version: 1
was generated by: iGEM to SBOL conversion
description: pSC101 heat sensitive origin of replication with deletion in par locus
created: 2012-08-25T11:00:00Z
modified: 2015-05-08T01:12:34Z
mutable provenance: Modified from BBa_K524000
owned by: james
owned by: myers
mutable description: A derivative of pSC101 origin of replication, this heat sensitive origin has the par locus deleted, therefore allowing a rapid loss of plasmid even under the permissive temperature.
discontinued: false
dominant: false
experience: experience/None
group u list: _689_
m user id: 0
owner id: 8758
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The par locus was deleted through excision of BBa_K524000 by SspI and StuI, purification of desired fragments and then
star: false
creator: HO Yuan Heng Trevor
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Direct Repeat 3 (DR3)
sequence annotation: repA101ts
sequence annotation: scar of removed par locus
sequence annotation: IHF binding sequence
sequence annotation: Inverted Repeat 2 (IR2) <-
sequence annotation: AT Rich Region
sequence annotation: Inverted Repeat 1 (IR1) ->
sequence annotation: DnaD protein binding sequence
sequence annotation: -10 promoter region of repAts
sequence annotation: Inverted Repeat 1 (IR1) <-
sequence annotation: Direct Repeat 2 (DR2)
sequence annotation: -35 promoter region of repAts
sequence annotation: Direct Repeat 1 (DR1)
sequence annotation: Inverted Repeat 2 (IR2) ->
sequence: https://synbiohub.org/public/igem/BBa_K524203_sequence/1

Direct Repeat 3 (DR3)
type: SequenceAnnotation
persistent identity: annotation2181028
display id: annotation2181028
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181028/range2181028/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181028/range2181028/1
type: Range
persistent identity: range2181028
display id: range2181028
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 1420
end: 1441
orientation: inline

repA101ts
type: SequenceAnnotation
persistent identity: annotation2181021
display id: annotation2181021
version: 1
top level: BBa_K524203
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181021/range2181021/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181021/range2181021/1
type: Range
persistent identity: range2181021
display id: range2181021
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 373
end: 1323
orientation: inline

scar of removed par locus
type: SequenceAnnotation
persistent identity: annotation2181034
display id: annotation2181034
version: 1
top level: BBa_K524203
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181034/range2181034/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181034/range2181034/1
type: Range
persistent identity: range2181034
display id: range2181034
version: 1
top level: BBa_K524203
direction: direction/forward
start: 1734
end: 1739
orientation: inline

IHF binding sequence
type: SequenceAnnotation
persistent identity: annotation2181031
display id: annotation2181031
version: 1
top level: BBa_K524203
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181031/range2181031/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181031/range2181031/1
type: Range
persistent identity: range2181031
display id: range2181031
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 1514
end: 1526
orientation: inline

Inverted Repeat 2 (IR2) <-
type: SequenceAnnotation
persistent identity: annotation2181023
display id: annotation2181023
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181023/range2181023/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181023/range2181023/1
type: Range
persistent identity: range2181023
display id: range2181023
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 1349
end: 1371
orientation: inline

AT Rich Region
type: SequenceAnnotation
persistent identity: annotation2181032
display id: annotation2181032
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181032/range2181032/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181032/range2181032/1
type: Range
persistent identity: range2181032
display id: range2181032
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 1492
end: 1547
orientation: inline

Inverted Repeat 1 (IR1) ->
type: SequenceAnnotation
persistent identity: annotation2181026
display id: annotation2181026
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181026/range2181026/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181026/range2181026/1
type: Range
persistent identity: range2181026
display id: range2181026
version: 1
top level: BBa_K524203
direction: direction/forward
start: 1371
end: 1384
orientation: inline

DnaD protein binding sequence
type: SequenceAnnotation
persistent identity: annotation2181033
display id: annotation2181033
version: 1
top level: BBa_K524203
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181033/range2181033/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181033/range2181033/1
type: Range
persistent identity: range2181033
display id: range2181033
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 1582
end: 1590
orientation: inline

-10 promoter region of repAts
type: SequenceAnnotation
persistent identity: annotation2181024
display id: annotation2181024
version: 1
top level: BBa_K524203
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181024/range2181024/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181024/range2181024/1
type: Range
persistent identity: range2181024
display id: range2181024
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 1354
end: 1359
orientation: inline

Inverted Repeat 1 (IR1) <-
type: SequenceAnnotation
persistent identity: annotation2181027
display id: annotation2181027
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181027/range2181027/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181027/range2181027/1
type: Range
persistent identity: range2181027
display id: range2181027
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 1385
end: 1397
orientation: inline

Direct Repeat 2 (DR2)
type: SequenceAnnotation
persistent identity: annotation2181029
display id: annotation2181029
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181029/range2181029/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181029/range2181029/1
type: Range
persistent identity: range2181029
display id: range2181029
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 1453
end: 1473
orientation: inline

-35 promoter region of repAts
type: SequenceAnnotation
persistent identity: annotation2181025
display id: annotation2181025
version: 1
top level: BBa_K524203
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181025/range2181025/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181025/range2181025/1
type: Range
persistent identity: range2181025
display id: range2181025
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 1376
end: 1381
orientation: inline

Direct Repeat 1 (DR1)
type: SequenceAnnotation
persistent identity: annotation2181030
display id: annotation2181030
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181030/range2181030/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181030/range2181030/1
type: Range
persistent identity: range2181030
display id: range2181030
version: 1
top level: BBa_K524203
direction: direction/reverse
start: 1474
end: 1494
orientation: inline

Inverted Repeat 2 (IR2) ->
type: SequenceAnnotation
persistent identity: annotation2181022
display id: annotation2181022
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181022/range2181022/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181022/range2181022/1
type: Range
persistent identity: range2181022
display id: range2181022
version: 1
top level: BBa_K524203
direction: direction/forward
start: 1325
end: 1348
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: stop
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

Suffix
type: ComponentDefinition
persistent identity: BBa G00001
display id: BBa_G00001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site suffix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks suffix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Suffix
sequence annotation: NotI
sequence annotation: SpeI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_G00001_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation1821544
display id: annotation1821544
version: 1
top level: Suffix
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1

https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
type: Range
persistent identity: range1821544
display id: range1821544
version: 1
top level: Suffix
direction: direction/forward
start: 1
end: 21
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1934228
display id: annotation1934228
version: 1
top level: Suffix
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1

https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
type: Range
persistent identity: range1934228
display id: range1934228
version: 1
top level: Suffix
direction: direction/forward
start: 9
end: 16
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1934226
display id: annotation1934226
version: 1
top level: Suffix
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1

https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
type: Range
persistent identity: range1934226
display id: range1934226
version: 1
top level: Suffix
direction: direction/forward
start: 2
end: 7
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1934227
display id: annotation1934227
version: 1
top level: Suffix
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1

https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
type: Range
persistent identity: range1934227
display id: range1934227
version: 1
top level: Suffix
direction: direction/forward
start: 16
end: 21
orientation: inline

Reporter +
type: ComponentDefinition
persistent identity: BBa K653001
display id: BBa_K653001
version: 1
was generated by: iGEM to SBOL conversion
description: GFP reporter plus Double Terminator
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:13:00Z
mutable provenance: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtg aaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatgg tgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaat tagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattac ctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg atgaactatacaaataataa ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc accttcgggtgggcctttctgcgtttata
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The iGEM Team Panama 2011 have to design and assemble the ends of an expression platform in order to bring up by rational designing a fuctional test platform for the expression of our Team favorite BioBrick part BBa_K653000 A.K. The Biosurfactator. We choose to assembly two existing BioBrick parts from the shop, we mean The Registry. Those parts are: a GFP reporter BBa_E0040 and a double terminator BBa_B0015. We assemble them to bring up a new composite part that will serve as a well characterized ends for a expression platform for several coding sequences like our RhlAB_BBa_K653000
discontinued: false
dominant: false
experience: experience/None
group u list: _830_
m user id: 0
owner id: 7119
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This GFP part was derived from the reporter device used by Elowitz's repressilator : GFPmut3* has a couple of mutations from wtGFP - S2R and S65G and S72A. Emerald and EGFP have S65T and multiple other mutations. The S2R mutation should have no effect. The Double terminator consisting of BBa_B0010 and BBa_B0012. This is the most commonly used terminator. It seems to be reliable. The assembling of this two parts was performed using the 3A Assembly Method.
star: false
creator: Ernesto G??mez
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_E0040
sequence annotation: BBa_B0015
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K653001_sequence/1

BBa_B0015
type: Component
persistent identity: component2146086
display id: component2146086
version: 1
top level: Reporter +
access: public
definition: BBa_B0015

BBa_E0040
type: Component
persistent identity: component2146079
display id: component2146079
version: 1
top level: Reporter +
access: public
definition: GFP

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2146086
display id: annotation2146086
version: 1
top level: Reporter +
location: https://synbiohub.org/public/igem/BBa_K653001/annotation2146086/range2146086/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K653001/annotation2146086/range2146086/1
type: Range
persistent identity: range2146086
display id: range2146086
version: 1
top level: Reporter +
direction: direction/reverse
start: 729
end: 857
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2146079
display id: annotation2146079
version: 1
top level: Reporter +
location: https://synbiohub.org/public/igem/BBa_K653001/annotation2146079/range2146079/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K653001/annotation2146079/range2146079/1
type: Range
persistent identity: range2146079
display id: range2146079
version: 1
top level: Reporter +
direction: direction/reverse
start: 1
end: 720
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem loop from bacteriophage T3
sequence annotation: identical to bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 36
end: 57
orientation: inline

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 28
end: 66
orientation: inline

BBa_K864050
type: ComponentDefinition
persistent identity: BBa K864050
display id: BBa_K864050
version: 1
was generated by: iGEM to SBOL conversion
description: pSC101 low copy origin of replication
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
mutable provenance: pSIM5
owned by: james
owned by: myers
mutable description: BBa_K864050 is a pSC101 origin of replication and is found in the pSB4*15 series of BioBrick vectors. This ori is an improvement of the ori found in all pSB4*5 plasmids, BBa_I50042. BBa_I50042 has been shown to have a higher copy number than expected.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: This ori was created by cloning pSC101ts from pSIM5 and making a V56A mutation in repA.
star: false
creator: Erik Lundin
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: -35 signal
sequence annotation: Repeated sequence
sequence annotation: AT rich region
sequence annotation: A>T mutation to remove SpeI
sequence annotation: Hairpin structure
sequence annotation: repA
sequence annotation: Repeat structure
sequence annotation: Tandem repeat
sequence annotation: pSC101 ori
sequence annotation: Repeat "4"
sequence annotation: par region
sequence annotation: Ins(GGC)
sequence annotation: Repeat "5"
sequence: https://synbiohub.org/public/igem/BBa_K864050_sequence/1

-35 signal
type: SequenceAnnotation
persistent identity: annotation2202479
display id: annotation2202479
version: 1
top level: BBa_K864050
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202479/range2202479/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202479/range2202479/1
type: Range
persistent identity: range2202479
display id: range2202479
version: 1
top level: BBa_K864050
direction: direction/forward
start: 692
end: 697
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202472
display id: annotation2202472
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202472/range2202472/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202472/range2202472/1
type: Range
persistent identity: range2202472
display id: range2202472
version: 1
top level: BBa_K864050
direction: direction/reverse
start: 603
end: 610
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation2202468
display id: annotation2202468
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202468/range2202468/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202468/range2202468/1
type: Range
persistent identity: range2202468
display id: range2202468
version: 1
top level: BBa_K864050
direction: direction/forward
start: 499
end: 581
orientation: inline

A>T mutation to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2202477
display id: annotation2202477
version: 1
top level: BBa_K864050
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202477/range2202477/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202477/range2202477/1
type: Range
persistent identity: range2202477
display id: range2202477
version: 1
top level: BBa_K864050
direction: direction/forward
start: 678
end: 678
orientation: inline

Hairpin structure
type: SequenceAnnotation
persistent identity: annotation2202483
display id: annotation2202483
version: 1
top level: BBa_K864050
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202483/range2202483/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202483/range2202483/1
type: Range
persistent identity: range2202483
display id: range2202483
version: 1
top level: BBa_K864050
direction: direction/forward
start: 1608
end: 1682
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2202482
display id: annotation2202482
version: 1
top level: BBa_K864050
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202482/range2202482/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202482/range2202482/1
type: Range
persistent identity: range2202482
display id: range2202482
version: 1
top level: BBa_K864050
direction: direction/forward
start: 750
end: 1700
orientation: inline

Hairpin structure
type: SequenceAnnotation
persistent identity: annotation2202478
display id: annotation2202478
version: 1
top level: BBa_K864050
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202478/range2202478/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202478/range2202478/1
type: Range
persistent identity: range2202478
display id: range2202478
version: 1
top level: BBa_K864050
direction: direction/forward
start: 683
end: 761
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202481
display id: annotation2202481
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202481/range2202481/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202481/range2202481/1
type: Range
persistent identity: range2202481
display id: range2202481
version: 1
top level: BBa_K864050
direction: direction/reverse
start: 712
end: 719
orientation: inline

Repeat structure
type: SequenceAnnotation
persistent identity: annotation2202467
display id: annotation2202467
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202467/range2202467/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202467/range2202467/1
type: Range
persistent identity: range2202467
display id: range2202467
version: 1
top level: BBa_K864050
direction: direction/forward
start: 232
end: 247
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202473
display id: annotation2202473
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202473/range2202473/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202473/range2202473/1
type: Range
persistent identity: range2202473
display id: range2202473
version: 1
top level: BBa_K864050
direction: direction/forward
start: 631
end: 652
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202471
display id: annotation2202471
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202471/range2202471/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202471/range2202471/1
type: Range
persistent identity: range2202471
display id: range2202471
version: 1
top level: BBa_K864050
direction: direction/forward
start: 601
end: 620
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2202465
display id: annotation2202465
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202465/range2202465/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202465/range2202465/1
type: Range
persistent identity: range2202465
display id: range2202465
version: 1
top level: BBa_K864050
direction: direction/forward
start: 1
end: 2216
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202469
display id: annotation2202469
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202469/range2202469/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202469/range2202469/1
type: Range
persistent identity: range2202469
display id: range2202469
version: 1
top level: BBa_K864050
direction: direction/forward
start: 578
end: 599
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202474
display id: annotation2202474
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202474/range2202474/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202474/range2202474/1
type: Range
persistent identity: range2202474
display id: range2202474
version: 1
top level: BBa_K864050
direction: direction/reverse
start: 635
end: 642
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202476
display id: annotation2202476
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202476/range2202476/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202476/range2202476/1
type: Range
persistent identity: range2202476
display id: range2202476
version: 1
top level: BBa_K864050
direction: direction/reverse
start: 676
end: 683
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2202475
display id: annotation2202475
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202475/range2202475/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202475/range2202475/1
type: Range
persistent identity: range2202475
display id: range2202475
version: 1
top level: BBa_K864050
direction: direction/forward
start: 673
end: 691
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation2202464
display id: annotation2202464
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202464/range2202464/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202464/range2202464/1
type: Range
persistent identity: range2202464
display id: range2202464
version: 1
top level: BBa_K864050
direction: direction/forward
start: 1
end: 368
orientation: inline

Ins(GGC)
type: SequenceAnnotation
persistent identity: annotation2202484
display id: annotation2202484
version: 1
top level: BBa_K864050
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202484/range2202484/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202484/range2202484/1
type: Range
persistent identity: range2202484
display id: range2202484
version: 1
top level: BBa_K864050
direction: direction/forward
start: 2141
end: 2143
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202470
display id: annotation2202470
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202470/range2202470/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202470/range2202470/1
type: Range
persistent identity: range2202470
display id: range2202470
version: 1
top level: BBa_K864050
direction: direction/reverse
start: 582
end: 589
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2202480
display id: annotation2202480
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202480/range2202480/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202480/range2202480/1
type: Range
persistent identity: range2202480
display id: range2202480
version: 1
top level: BBa_K864050
direction: direction/forward
start: 702
end: 723
orientation: inline

Repeat structure
type: SequenceAnnotation
persistent identity: annotation2202466
display id: annotation2202466
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202466/range2202466/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202466/range2202466/1
type: Range
persistent identity: range2202466
display id: range2202466
version: 1
top level: BBa_K864050
direction: direction/forward
start: 190
end: 204
orientation: inline

R6K
type: ComponentDefinition
persistent identity: BBa J61001
display id: BBa_J61001
version: 1
was generated by: iGEM to SBOL conversion
description: [R6K] Origin of replication
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is Bca1011 ---- ca1011F Forward Biobricking of R6K GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg ca1011R Reverse biobricking of R6K ccgctactagtaGCAGTTCAACCTGTTGATAG
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature. {pSB1A2-Bca1011}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: R6K
sequence: https://synbiohub.org/public/igem/BBa_J61001_sequence/1

R6K
type: SequenceAnnotation
persistent identity: annotation1892271
display id: annotation1892271
version: 1
top level: R6K
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1

https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1
type: Range
persistent identity: range1892271
display id: range1892271
version: 1
top level: R6K
direction: direction/forward
start: 27
end: 406
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

pSC101
type: ComponentDefinition
persistent identity: BBa I50041
display id: BBa_I50041
version: 1
was generated by: iGEM to SBOL conversion
description: pSC101 origin of replication, reverse
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:42Z
owned by: james
owned by: myers
mutable description: pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. This part is the reverse copy of BBa_I50040.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: difference from canonical pSC101 sequence in Genbank
sequence annotation: inverted repeat
sequence annotation: promoter region
sequence annotation: AT rich region
sequence annotation: rep promoter -35
sequence annotation: RBS
sequence annotation: terminator
sequence annotation: Mutated SpeI site
sequence annotation: putative polyA
sequence annotation: par region
sequence annotation: direct repeat
sequence annotation: Tn3 integration site
sequence annotation: repA
sequence annotation: rep promoter -10
sequence: https://synbiohub.org/public/igem/BBa_I50041_sequence/1

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080859
display id: annotation2080859
version: 1
top level: pSC101
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080859/range2080859/1

https://synbiohub.org/public/igem/BBa_I50041/annotation2080859/range2080859/1
type: Range
persistent identity: range2080859
display id: range2080859
version: 1
top level: pSC101
direction: direction/forward
start: 383
end: 383
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932231
display id: annotation1932231
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932231/range1932231/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932231/range1932231/1
type: Range
persistent identity: range1932231
display id: range1932231
version: 1
top level: pSC101
direction: direction/reverse
start: 1823
end: 1846
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation1932220
display id: annotation1932220
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932220/range1932220/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932220/range1932220/1
type: Range
persistent identity: range1932220
display id: range1932220
version: 1
top level: pSC101
direction: direction/reverse
start: 1173
end: 1192
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation1932228
display id: annotation1932228
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932228/range1932228/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932228/range1932228/1
type: Range
persistent identity: range1932228
display id: range1932228
version: 1
top level: pSC101
direction: direction/reverse
start: 1321
end: 1403
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932221
display id: annotation1932221
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932221/range1932221/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932221/range1932221/1
type: Range
persistent identity: range1932221
display id: range1932221
version: 1
top level: pSC101
direction: direction/reverse
start: 1179
end: 1200
orientation: inline

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080856
display id: annotation2080856
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080856/range2080856/1

https://synbiohub.org/public/igem/BBa_I50041/annotation2080856/range2080856/1
type: Range
persistent identity: range2080856
display id: range2080856
version: 1
top level: pSC101
direction: direction/forward
start: 36
end: 36
orientation: inline

rep promoter -35
type: SequenceAnnotation
persistent identity: annotation1932223
display id: annotation1932223
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932223/range1932223/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932223/range1932223/1
type: Range
persistent identity: range1932223
display id: range1932223
version: 1
top level: pSC101
direction: direction/reverse
start: 1205
end: 1210
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932232
display id: annotation1932232
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932232/range1932232/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932232/range1932232/1
type: Range
persistent identity: range1932232
display id: range1932232
version: 1
top level: pSC101
direction: direction/reverse
start: 1857
end: 1873
orientation: inline

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080857
display id: annotation2080857
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080857/range2080857/1

https://synbiohub.org/public/igem/BBa_I50041/annotation2080857/range2080857/1
type: Range
persistent identity: range2080857
display id: range2080857
version: 1
top level: pSC101
direction: direction/forward
start: 126
end: 126
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932234
display id: annotation1932234
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932234/range1932234/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932234/range1932234/1
type: Range
persistent identity: range1932234
display id: range1932234
version: 1
top level: pSC101
direction: direction/reverse
start: 1886
end: 1899
orientation: inline

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080858
display id: annotation2080858
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080858/range2080858/1

https://synbiohub.org/public/igem/BBa_I50041/annotation2080858/range2080858/1
type: Range
persistent identity: range2080858
display id: range2080858
version: 1
top level: pSC101
direction: direction/forward
start: 288
end: 288
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1932218
display id: annotation1932218
version: 1
top level: pSC101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932218/range1932218/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932218/range1932218/1
type: Range
persistent identity: range1932218
display id: range1932218
version: 1
top level: pSC101
direction: direction/reverse
start: 1154
end: 1159
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1932235
display id: annotation1932235
version: 1
top level: pSC101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932235/range1932235/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932235/range1932235/1
type: Range
persistent identity: range1932235
display id: range1932235
version: 1
top level: pSC101
direction: direction/reverse
start: 1963
end: 1992
orientation: inline

Mutated SpeI site
type: SequenceAnnotation
persistent identity: annotation1932224
display id: annotation1932224
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932224/range1932224/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932224/range1932224/1
type: Range
persistent identity: range1932224
display id: range1932224
version: 1
top level: pSC101
direction: direction/reverse
start: 1219
end: 1224
orientation: inline

putative polyA
type: SequenceAnnotation
persistent identity: annotation1932215
display id: annotation1932215
version: 1
top level: pSC101
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932215/range1932215/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932215/range1932215/1
type: Range
persistent identity: range1932215
display id: range1932215
version: 1
top level: pSC101
direction: direction/reverse
start: 76
end: 91
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation1932229
display id: annotation1932229
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932229/range1932229/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932229/range1932229/1
type: Range
persistent identity: range1932229
display id: range1932229
version: 1
top level: pSC101
direction: direction/reverse
start: 1534
end: 1901
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1932227
display id: annotation1932227
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932227/range1932227/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932227/range1932227/1
type: Range
persistent identity: range1932227
display id: range1932227
version: 1
top level: pSC101
direction: direction/reverse
start: 1303
end: 1322
orientation: inline

Tn3 integration site
type: SequenceAnnotation
persistent identity: annotation1932233
display id: annotation1932233
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932233/range1932233/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932233/range1932233/1
type: Range
persistent identity: range1932233
display id: range1932233
version: 1
top level: pSC101
direction: direction/reverse
start: 1876
end: 1880
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932230
display id: annotation1932230
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932230/range1932230/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932230/range1932230/1
type: Range
persistent identity: range1932230
display id: range1932230
version: 1
top level: pSC101
direction: direction/reverse
start: 1800
end: 1817
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932217
display id: annotation1932217
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932217/range1932217/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932217/range1932217/1
type: Range
persistent identity: range1932217
display id: range1932217
version: 1
top level: pSC101
direction: direction/reverse
start: 1154
end: 1176
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation1932216
display id: annotation1932216
version: 1
top level: pSC101
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932216/range1932216/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932216/range1932216/1
type: Range
persistent identity: range1932216
display id: range1932216
version: 1
top level: pSC101
direction: direction/reverse
start: 202
end: 1152
orientation: inline

rep promoter -10
type: SequenceAnnotation
persistent identity: annotation1932222
display id: annotation1932222
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932222/range1932222/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932222/range1932222/1
type: Range
persistent identity: range1932222
display id: range1932222
version: 1
top level: pSC101
direction: direction/reverse
start: 1183
end: 1188
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1932226
display id: annotation1932226
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932226/range1932226/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932226/range1932226/1
type: Range
persistent identity: range1932226
display id: range1932226
version: 1
top level: pSC101
direction: direction/reverse
start: 1282
end: 1301
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1932219
display id: annotation1932219
version: 1
top level: pSC101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932219/range1932219/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932219/range1932219/1
type: Range
persistent identity: range1932219
display id: range1932219
version: 1
top level: pSC101
direction: direction/reverse
start: 1156
end: 1168
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1932225
display id: annotation1932225
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932225/range1932225/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932225/range1932225/1
type: Range
persistent identity: range1932225
display id: range1932225
version: 1
top level: pSC101
direction: direction/reverse
start: 1250
end: 1269
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0043
display id: BBa_B0043
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (forward)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Generated T overhang
sequence annotation: Nt.BstNB I recognition site
sequence annotation: Topoisomerase I recognition site
sequence annotation: Arbitrary sequence (could be any bases)
sequence: https://synbiohub.org/public/igem/BBa_B0043_sequence/1

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797104
display id: annotation1797104
version: 1
top level: TOPO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1

https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
type: Range
persistent identity: range1797104
display id: range1797104
version: 1
top level: TOPO
direction: direction/forward
start: 5
end: 5
orientation: inline

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797102
display id: annotation1797102
version: 1
top level: TOPO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1

https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
type: Range
persistent identity: range1797102
display id: range1797102
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797101
display id: annotation1797101
version: 1
top level: TOPO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1

https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
type: Range
persistent identity: range1797101
display id: range1797101
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797103
display id: annotation1797103
version: 1
top level: TOPO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1

https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
type: Range
persistent identity: range1797103
display id: range1797103
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

B-H-R plas
type: ComponentDefinition
persistent identity: BBa K125020
display id: BBa_K125020
version: 1
was generated by: iGEM to SBOL conversion
description: lac promoter/rbs/rep/oriV/lac promoter/rbs/aadA/oriT
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: This part was derived from RSF1010 and RP4.
owned by: james
owned by: myers
mutable description: This is an intermediate construction step for the construction of the broad-host-range plasmid derived from RSF1010. The coding region was inserted into pSB1A3.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: I am having trouble inserting this part into the Base Vector so the construct will be tested while still in pSB1A3.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_K125500
component: BBa_K125510
component: BBa_K125310
component: BBa_I14032
component: BBa_K125340
component: BBa_K125320
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K125500
sequence annotation: BBa_K125320
sequence annotation: BBa_K125340
sequence annotation: BBa_K125510
sequence annotation: BBa_I14032
sequence annotation: BBa_K125310
sequence: https://synbiohub.org/public/igem/BBa_K125020_sequence/1

BBa_K125500
type: Component
persistent identity: component1992599
display id: component1992599
version: 1
top level: B-H-R plas
access: public
definition: BBa_K125500

BBa_K125510
type: Component
persistent identity: component1992585
display id: component1992585
version: 1
top level: B-H-R plas
access: public
definition: Rep

BBa_K125310
type: Component
persistent identity: component1992597
display id: component1992597
version: 1
top level: B-H-R plas
access: public
definition: slr2016 SS

BBa_I14032
type: Component
persistent identity: component1992576
display id: component1992576
version: 1
top level: B-H-R plas
access: public
definition: P(Lac) IQ

BBa_K125340
type: Component
persistent identity: component1992589
display id: component1992589
version: 1
top level: B-H-R plas
access: public
definition: OriV

BBa_K125320
type: Component
persistent identity: component1992600
display id: component1992600
version: 1
top level: B-H-R plas
access: public
definition: OriT-RP4

BBa_I14032
type: Component
persistent identity: component1992593
display id: component1992593
version: 1
top level: B-H-R plas
access: public
definition: P(Lac) IQ

BBa_B0034
type: Component
persistent identity: component1992595
display id: component1992595
version: 1
top level: B-H-R plas
access: public
definition: BBa_B0034

BBa_B0034
type: Component
persistent identity: component1992578
display id: component1992578
version: 1
top level: B-H-R plas
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1992595
display id: annotation1992595
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992595/range1992595/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125020/annotation1992595/range1992595/1
type: Range
persistent identity: range1992595
display id: range1992595
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 3936
end: 3947
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1992578
display id: annotation1992578
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992578/range1992578/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125020/annotation1992578/range1992578/1
type: Range
persistent identity: range1992578
display id: range1992578
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 46
end: 57
orientation: inline

BBa_K125500
type: SequenceAnnotation
persistent identity: annotation1992599
display id: annotation1992599
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992599/range1992599/1
component: BBa_K125500

https://synbiohub.org/public/igem/BBa_K125020/annotation1992599/range1992599/1
type: Range
persistent identity: range1992599
display id: range1992599
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 4017
end: 4734
orientation: inline

BBa_K125320
type: SequenceAnnotation
persistent identity: annotation1992600
display id: annotation1992600
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992600/range1992600/1
component: BBa_K125320

https://synbiohub.org/public/igem/BBa_K125020/annotation1992600/range1992600/1
type: Range
persistent identity: range1992600
display id: range1992600
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 4743
end: 4841
orientation: inline

BBa_K125340
type: SequenceAnnotation
persistent identity: annotation1992589
display id: annotation1992589
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992589/range1992589/1
component: BBa_K125340

https://synbiohub.org/public/igem/BBa_K125020/annotation1992589/range1992589/1
type: Range
persistent identity: range1992589
display id: range1992589
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 3459
end: 3882
orientation: inline

BBa_K125510
type: SequenceAnnotation
persistent identity: annotation1992585
display id: annotation1992585
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992585/range1992585/1
component: BBa_K125510

https://synbiohub.org/public/igem/BBa_K125020/annotation1992585/range1992585/1
type: Range
persistent identity: range1992585
display id: range1992585
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 66
end: 3450
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1992593
display id: annotation1992593
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992593/range1992593/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125020/annotation1992593/range1992593/1
type: Range
persistent identity: range1992593
display id: range1992593
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 3891
end: 3927
orientation: inline

BBa_K125310
type: SequenceAnnotation
persistent identity: annotation1992597
display id: annotation1992597
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992597/range1992597/1
component: BBa_K125310

https://synbiohub.org/public/igem/BBa_K125020/annotation1992597/range1992597/1
type: Range
persistent identity: range1992597
display id: range1992597
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 3956
end: 4008
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1992576
display id: annotation1992576
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992576/range1992576/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125020/annotation1992576/range1992576/1
type: Range
persistent identity: range1992576
display id: range1992576
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 1
end: 37
orientation: inline

pUC19 ori
type: ComponentDefinition
persistent identity: BBa I50022
display id: BBa_I50022
version: 1
was generated by: iGEM to SBOL conversion
description: Minimal pUC19-derived high copy replication origin
created: 2008-01-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: pSB1A3
owned by: james
owned by: myers
mutable description: High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites. Lacks mutations that render BBa_I50020 nonfunctional as a replication origin.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We had to leave several restriction sites in because point mutations that eliminated these sites were found to render the origin nonfunctional.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RNAI transcript
sequence annotation: RNAI transcript promoter -35
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: RNAII transcript
sequence annotation: rep (pMB1)
sequence annotation: RNAI transcript promoter -10
sequence annotation: origin of replication
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_I50022_sequence/1

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1959122
display id: annotation1959122
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959122/range1959122/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959122/range1959122/1
type: Range
persistent identity: range1959122
display id: range1959122
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 66
end: 173
orientation: inline

RNAI transcript promoter -35
type: SequenceAnnotation
persistent identity: annotation1959124
display id: annotation1959124
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959124/range1959124/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959124/range1959124/1
type: Range
persistent identity: range1959124
display id: range1959124
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 204
end: 209
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1959118
display id: annotation1959118
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959118/range1959118/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959118/range1959118/1
type: Range
persistent identity: range1959118
display id: range1959118
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 32
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1959120
display id: annotation1959120
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959120/range1959120/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959120/range1959120/1
type: Range
persistent identity: range1959120
display id: range1959120
version: 1
top level: pUC19 ori
direction: direction/forward
start: 48
end: 53
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1959121
display id: annotation1959121
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959121/range1959121/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959121/range1959121/1
type: Range
persistent identity: range1959121
display id: range1959121
version: 1
top level: pUC19 ori
direction: direction/forward
start: 63
end: 615
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1959117
display id: annotation1959117
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959117/range1959117/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959117/range1959117/1
type: Range
persistent identity: range1959117
display id: range1959117
version: 1
top level: pUC19 ori
direction: direction/forward
start: 16
end: 630
orientation: inline

RNAI transcript promoter -10
type: SequenceAnnotation
persistent identity: annotation1959123
display id: annotation1959123
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959123/range1959123/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959123/range1959123/1
type: Range
persistent identity: range1959123
display id: range1959123
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 182
end: 187
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1959119
display id: annotation1959119
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959119/range1959119/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959119/range1959119/1
type: Range
persistent identity: range1959119
display id: range1959119
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 615
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1959125
display id: annotation1959125
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959125/range1959125/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959125/range1959125/1
type: Range
persistent identity: range1959125
display id: range1959125
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 615
end: 615
orientation: inline

BBa_I50050
type: ComponentDefinition
persistent identity: BBa I50050
display id: BBa_I50050
version: 1
was generated by: iGEM to SBOL conversion
description: R6K replication origin
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:42Z
owned by: james
owned by: myers
mutable description: R6K minimal origin of replication. Only contains gamma origin and requires pir+ strain for replication. Copy number is dependent on amount of pir protein in the cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: R6K origin
sequence annotation: gamma origin
sequence: https://synbiohub.org/public/igem/BBa_I50050_sequence/1

R6K origin
type: SequenceAnnotation
persistent identity: annotation1796245
display id: annotation1796245
version: 1
top level: BBa_I50050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50050/annotation1796245/range1796245/1

https://synbiohub.org/public/igem/BBa_I50050/annotation1796245/range1796245/1
type: Range
persistent identity: range1796245
display id: range1796245
version: 1
top level: BBa_I50050
direction: direction/forward
start: 1
end: 306
orientation: inline

gamma origin
type: SequenceAnnotation
persistent identity: annotation1796246
display id: annotation1796246
version: 1
top level: BBa_I50050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50050/annotation1796246/range1796246/1

https://synbiohub.org/public/igem/BBa_I50050/annotation1796246/range1796246/1
type: Range
persistent identity: range1796246
display id: range1796246
version: 1
top level: BBa_I50050
direction: direction/forward
start: 37
end: 312
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0044
display id: BBa_B0044
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (reverse)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Nt.BstNB I recognition site
sequence: https://synbiohub.org/public/igem/BBa_B0044_sequence/1

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797128
display id: annotation1797128
version: 1
top level: TOPO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1

https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
type: Range
persistent identity: range1797128
display id: range1797128
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797129
display id: annotation1797129
version: 1
top level: TOPO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1

https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
type: Range
persistent identity: range1797129
display id: range1797129
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 9
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797127
display id: annotation1797127
version: 1
top level: TOPO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1

https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
type: Range
persistent identity: range1797127
display id: range1797127
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797126
display id: annotation1797126
version: 1
top level: TOPO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1

https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
type: Range
persistent identity: range1797126
display id: range1797126
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Ori
type: ComponentDefinition
persistent identity: BBa K320000
display id: BBa_K320000
version: 1
was generated by: iGEM to SBOL conversion
description: Asaia replication origin
created: 2010-10-16T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: test1
discontinued: false
dominant: false
experience: experience/None
group u list: _439_
m user id: 0
owner id: 7149
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: test
star: false
creator: Perrudet Christian
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Asaia replication origin
sequence: https://synbiohub.org/public/igem/BBa_K320000_sequence/1

Asaia replication origin
type: SequenceAnnotation
persistent identity: annotation2091381
display id: annotation2091381
version: 1
top level: Ori
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K320000/annotation2091381/range2091381/1

https://synbiohub.org/public/igem/BBa_K320000/annotation2091381/range2091381/1
type: Range
persistent identity: range2091381
display id: range2091381
version: 1
top level: Ori
direction: direction/forward
start: 371
end: 1046
orientation: inline

pSC101ts
type: ComponentDefinition
persistent identity: BBa K864051
display id: BBa_K864051
version: 1
was generated by: iGEM to SBOL conversion
description: pSC101ts temperature sensitive low copy origin of replication
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:37Z
mutable provenance: pSIM5
owned by: james
owned by: myers
mutable description: pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: -
star: false
creator: Erik Lundin
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Repeated sequence
sequence annotation: Tandem repeat
sequence annotation: Hairpin structure
sequence annotation: Repeat "4"
sequence annotation: Ins(GGC)
sequence annotation: A56V ts mutation
sequence annotation: Repeat "5"
sequence annotation: -35 signal
sequence annotation: Repeat structure
sequence annotation: AT rich region
sequence annotation: par region
sequence annotation: repA
sequence annotation: pSC101ts ori
sequence annotation: A>T mutation to remove SpeI
sequence: https://synbiohub.org/public/igem/BBa_K864051_sequence/1

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202700
display id: annotation2202700
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202700/range2202700/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202700/range2202700/1
type: Range
persistent identity: range2202700
display id: range2202700
version: 1
top level: pSC101ts
direction: direction/reverse
start: 603
end: 610
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202697
display id: annotation2202697
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202697/range2202697/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202697/range2202697/1
type: Range
persistent identity: range2202697
display id: range2202697
version: 1
top level: pSC101ts
direction: direction/forward
start: 578
end: 599
orientation: inline

Hairpin structure
type: SequenceAnnotation
persistent identity: annotation2202712
display id: annotation2202712
version: 1
top level: pSC101ts
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202712/range2202712/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202712/range2202712/1
type: Range
persistent identity: range2202712
display id: range2202712
version: 1
top level: pSC101ts
direction: direction/forward
start: 1608
end: 1682
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2202703
display id: annotation2202703
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202703/range2202703/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202703/range2202703/1
type: Range
persistent identity: range2202703
display id: range2202703
version: 1
top level: pSC101ts
direction: direction/forward
start: 671
end: 693
orientation: inline

Ins(GGC)
type: SequenceAnnotation
persistent identity: annotation2202713
display id: annotation2202713
version: 1
top level: pSC101ts
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202713/range2202713/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202713/range2202713/1
type: Range
persistent identity: range2202713
display id: range2202713
version: 1
top level: pSC101ts
direction: direction/forward
start: 2141
end: 2143
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202699
display id: annotation2202699
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202699/range2202699/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202699/range2202699/1
type: Range
persistent identity: range2202699
display id: range2202699
version: 1
top level: pSC101ts
direction: direction/forward
start: 601
end: 620
orientation: inline

A56V ts mutation
type: SequenceAnnotation
persistent identity: annotation2202688
display id: annotation2202688
version: 1
top level: pSC101ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202688/range2202688/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202688/range2202688/1
type: Range
persistent identity: range2202688
display id: range2202688
version: 1
top level: pSC101ts
direction: direction/forward
start: 915
end: 917
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202702
display id: annotation2202702
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202702/range2202702/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202702/range2202702/1
type: Range
persistent identity: range2202702
display id: range2202702
version: 1
top level: pSC101ts
direction: direction/reverse
start: 635
end: 642
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2202710
display id: annotation2202710
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202710/range2202710/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202710/range2202710/1
type: Range
persistent identity: range2202710
display id: range2202710
version: 1
top level: pSC101ts
direction: direction/forward
start: 702
end: 723
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202705
display id: annotation2202705
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202705/range2202705/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202705/range2202705/1
type: Range
persistent identity: range2202705
display id: range2202705
version: 1
top level: pSC101ts
direction: direction/reverse
start: 676
end: 683
orientation: inline

-35 signal
type: SequenceAnnotation
persistent identity: annotation2202708
display id: annotation2202708
version: 1
top level: pSC101ts
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202708/range2202708/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202708/range2202708/1
type: Range
persistent identity: range2202708
display id: range2202708
version: 1
top level: pSC101ts
direction: direction/forward
start: 692
end: 697
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202701
display id: annotation2202701
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202701/range2202701/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202701/range2202701/1
type: Range
persistent identity: range2202701
display id: range2202701
version: 1
top level: pSC101ts
direction: direction/forward
start: 631
end: 652
orientation: inline

Repeat structure
type: SequenceAnnotation
persistent identity: annotation2202695
display id: annotation2202695
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202695/range2202695/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202695/range2202695/1
type: Range
persistent identity: range2202695
display id: range2202695
version: 1
top level: pSC101ts
direction: direction/forward
start: 232
end: 247
orientation: inline

Repeat structure
type: SequenceAnnotation
persistent identity: annotation2202693
display id: annotation2202693
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202693/range2202693/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202693/range2202693/1
type: Range
persistent identity: range2202693
display id: range2202693
version: 1
top level: pSC101ts
direction: direction/forward
start: 190
end: 204
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation2202696
display id: annotation2202696
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202696/range2202696/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202696/range2202696/1
type: Range
persistent identity: range2202696
display id: range2202696
version: 1
top level: pSC101ts
direction: direction/forward
start: 499
end: 581
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation2202692
display id: annotation2202692
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202692/range2202692/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202692/range2202692/1
type: Range
persistent identity: range2202692
display id: range2202692
version: 1
top level: pSC101ts
direction: direction/forward
start: 1
end: 368
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2202709
display id: annotation2202709
version: 1
top level: pSC101ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202709/range2202709/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202709/range2202709/1
type: Range
persistent identity: range2202709
display id: range2202709
version: 1
top level: pSC101ts
direction: direction/forward
start: 750
end: 1700
orientation: inline

pSC101ts ori
type: SequenceAnnotation
persistent identity: annotation2202690
display id: annotation2202690
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202690/range2202690/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202690/range2202690/1
type: Range
persistent identity: range2202690
display id: range2202690
version: 1
top level: pSC101ts
direction: direction/forward
start: 1
end: 2216
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202698
display id: annotation2202698
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202698/range2202698/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202698/range2202698/1
type: Range
persistent identity: range2202698
display id: range2202698
version: 1
top level: pSC101ts
direction: direction/reverse
start: 582
end: 589
orientation: inline

A>T mutation to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2202706
display id: annotation2202706
version: 1
top level: pSC101ts
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202706/range2202706/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202706/range2202706/1
type: Range
persistent identity: range2202706
display id: range2202706
version: 1
top level: pSC101ts
direction: direction/forward
start: 678
end: 678
orientation: inline

Hairpin structure
type: SequenceAnnotation
persistent identity: annotation2202707
display id: annotation2202707
version: 1
top level: pSC101ts
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202707/range2202707/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202707/range2202707/1
type: Range
persistent identity: range2202707
display id: range2202707
version: 1
top level: pSC101ts
direction: direction/forward
start: 683
end: 761
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202711
display id: annotation2202711
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202711/range2202711/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202711/range2202711/1
type: Range
persistent identity: range2202711
display id: range2202711
version: 1
top level: pSC101ts
direction: direction/reverse
start: 712
end: 719
orientation: inline

VR
type: ComponentDefinition
persistent identity: BBa G00102
display id: BBa_G00102
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick primer annealing site (VR binding site)
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00102_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1961229
display id: annotation1961229
version: 1
top level: VR
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1

https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
type: Range
persistent identity: range1961229
display id: range1961229
version: 1
top level: VR
direction: direction/reverse
start: 1
end: 20
orientation: inline

BBa_K863201
type: ComponentDefinition
persistent identity: BBa K863201
display id: BBa_K863201
version: 1
was generated by: iGEM to SBOL conversion
description: 3' UTR site of alcohol oxidase 1 gene (aox1)
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
mutable provenance: The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 3' UTR site of alcohol oxidase 1 gene (AOX1) from K. pastoris used as a recombination site.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: primer sequences are coming soon
star: false
creator: Julia Schirmacher
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K863201_sequence/1

pSC101
type: ComponentDefinition
persistent identity: BBa I50040
display id: BBa_I50040
version: 1
was generated by: iGEM to SBOL conversion
description: nonfunctional pSC101 origin of replication
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:42Z
owned by: james
owned by: myers
mutable description: pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: direct repeat
sequence annotation: inverted repeat
sequence annotation: AT rich region
sequence annotation: par region
sequence annotation: potential hairpin
sequence annotation: T->A mutation to remove MfeI site
sequence annotation: promoter region
sequence annotation: 11bp sequence repeated 4 times in oriC
sequence annotation: repA
sequence annotation: -10 signal functional promoter
sequence annotation: RBS
sequence annotation: A->T mutation to remove MfeI site
sequence: https://synbiohub.org/public/igem/BBa_I50040_sequence/1

direct repeat
type: SequenceAnnotation
persistent identity: annotation1796226
display id: annotation1796226
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796226/range1796226/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796226/range1796226/1
type: Range
persistent identity: range1796226
display id: range1796226
version: 1
top level: pSC101
direction: direction/forward
start: 578
end: 601
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1796221
display id: annotation1796221
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796221/range1796221/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796221/range1796221/1
type: Range
persistent identity: range1796221
display id: range1796221
version: 1
top level: pSC101
direction: direction/forward
start: 180
end: 189
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation1796225
display id: annotation1796225
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796225/range1796225/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796225/range1796225/1
type: Range
persistent identity: range1796225
display id: range1796225
version: 1
top level: pSC101
direction: direction/forward
start: 503
end: 581
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation1796222
display id: annotation1796222
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796222/range1796222/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796222/range1796222/1
type: Range
persistent identity: range1796222
display id: range1796222
version: 1
top level: pSC101
direction: direction/forward
start: 1
end: 368
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1796228
display id: annotation1796228
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796228/range1796228/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796228/range1796228/1
type: Range
persistent identity: range1796228
display id: range1796228
version: 1
top level: pSC101
direction: direction/forward
start: 631
end: 654
orientation: inline

potential hairpin
type: SequenceAnnotation
persistent identity: annotation1796231
display id: annotation1796231
version: 1
top level: pSC101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796231/range1796231/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796231/range1796231/1
type: Range
persistent identity: range1796231
display id: range1796231
version: 1
top level: pSC101
direction: direction/forward
start: 683
end: 761
orientation: inline

T->A mutation to remove MfeI site
type: SequenceAnnotation
persistent identity: annotation1880624
display id: annotation1880624
version: 1
top level: pSC101
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1880624/range1880624/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1880624/range1880624/1
type: Range
persistent identity: range1880624
display id: range1880624
version: 1
top level: pSC101
direction: direction/forward
start: 742
end: 742
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1796227
display id: annotation1796227
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796227/range1796227/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796227/range1796227/1
type: Range
persistent identity: range1796227
display id: range1796227
version: 1
top level: pSC101
direction: direction/forward
start: 603
end: 620
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation1796230
display id: annotation1796230
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796230/range1796230/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796230/range1796230/1
type: Range
persistent identity: range1796230
display id: range1796230
version: 1
top level: pSC101
direction: direction/forward
start: 710
end: 729
orientation: inline

11bp sequence repeated 4 times in oriC
type: SequenceAnnotation
persistent identity: annotation1796224
display id: annotation1796224
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796224/range1796224/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796224/range1796224/1
type: Range
persistent identity: range1796224
display id: range1796224
version: 1
top level: pSC101
direction: direction/forward
start: 482
end: 492
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation1796236
display id: annotation1796236
version: 1
top level: pSC101
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796236/range1796236/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796236/range1796236/1
type: Range
persistent identity: range1796236
display id: range1796236
version: 1
top level: pSC101
direction: direction/forward
start: 750
end: 1700
orientation: inline

-10 signal functional promoter
type: SequenceAnnotation
persistent identity: annotation1796235
display id: annotation1796235
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796235/range1796235/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796235/range1796235/1
type: Range
persistent identity: range1796235
display id: range1796235
version: 1
top level: pSC101
direction: direction/forward
start: 948
end: 953
orientation: inline

potential hairpin
type: SequenceAnnotation
persistent identity: annotation1796238
display id: annotation1796238
version: 1
top level: pSC101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796238/range1796238/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796238/range1796238/1
type: Range
persistent identity: range1796238
display id: range1796238
version: 1
top level: pSC101
direction: direction/forward
start: 2063
end: 2088
orientation: inline

potential hairpin
type: SequenceAnnotation
persistent identity: annotation1796237
display id: annotation1796237
version: 1
top level: pSC101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796237/range1796237/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796237/range1796237/1
type: Range
persistent identity: range1796237
display id: range1796237
version: 1
top level: pSC101
direction: direction/forward
start: 1608
end: 1682
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1796233
display id: annotation1796233
version: 1
top level: pSC101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796233/range1796233/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796233/range1796233/1
type: Range
persistent identity: range1796233
display id: range1796233
version: 1
top level: pSC101
direction: direction/forward
start: 734
end: 746
orientation: inline

A->T mutation to remove MfeI site
type: SequenceAnnotation
persistent identity: annotation1880623
display id: annotation1880623
version: 1
top level: pSC101
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1880623/range1880623/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1880623/range1880623/1
type: Range
persistent identity: range1880623
display id: range1880623
version: 1
top level: pSC101
direction: direction/forward
start: 708
end: 708
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1796234
display id: annotation1796234
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796234/range1796234/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796234/range1796234/1
type: Range
persistent identity: range1796234
display id: range1796234
version: 1
top level: pSC101
direction: direction/reverse
start: 726
end: 749
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1796223
display id: annotation1796223
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796223/range1796223/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796223/range1796223/1
type: Range
persistent identity: range1796223
display id: range1796223
version: 1
top level: pSC101
direction: direction/reverse
start: 191
end: 201
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1796229
display id: annotation1796229
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796229/range1796229/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796229/range1796229/1
type: Range
persistent identity: range1796229
display id: range1796229
version: 1
top level: pSC101
direction: direction/forward
start: 702
end: 723
orientation: inline

-10 signal functional promoter
type: SequenceAnnotation
persistent identity: annotation1796232
display id: annotation1796232
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796232/range1796232/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796232/range1796232/1
type: Range
persistent identity: range1796232
display id: range1796232
version: 1
top level: pSC101
direction: direction/forward
start: 723
end: 727
orientation: inline

https://synbiohub.org/public/igem/BBa_K533000_sequence/1
type: Sequence
persistent identity: BBa K533000 sequence
display id: BBa_K533000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgctagcggagtgtatactggcttactatgttggcactgatgagggtgtcagtgaagtgcttcatgtggcaggagaaaaaaggctgcaccggtgcgtcagcagaatatgtgatacaggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaatcagataaaatatttctagttttcagtgcaatttatctcttcaaatgtagcacctgaagtcagccccatacgatataagttgta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50022_sequence/1
type: Sequence
persistent identity: BBa I50022 sequence
display id: BBa_I50022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125340_sequence/1
type: Sequence
persistent identity: BBa K125340 sequence
display id: BBa_K125340_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacccctgcaataactgtcacgcccccctgcaataactgtcacgaacccctgcaataactgtcacgcccccaaacctgcaaacccagcaggggcgggggctggcggggtgttggaaaaatccatccatgattatctaagaataatccactaggcgcggttatcagcgcccttgtggggcgctgctgcccttgcccaatatgcccggccagaggccggatagctggtctattcgctgcgctaggctacacaccgccccaccgctgcgcggcagggggaaaggcgggcaaagcccgctaaaccccacaccaaaccccgcagaaatacgctggagcgcttttagccgctttagcggcctttccccctacccgaagggtgggggcgcgtgtgcagccccgcagggcctgtctcggtcgatcattcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125310_sequence/1
type: Sequence
persistent identity: BBa K125310 sequence
display id: BBa_K125310_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggcagcaaaacaactatggaaaattttcaatcctagaccgatgaagggtgga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112125_sequence/1
type: Sequence
persistent identity: BBa K112125 sequence
display id: BBa_K112125_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgcttcctcgctcactcgaccgggagggttcgagaagggggggcaccccccttcggcgtgcgcggtcacgcgcacagggcgcagccctggttaaaaacaaggtttataaatattggtttaaaagcaggttaaaagacaggttagcggtggccgaaaaacgggcggaaacccttgcaaatgctggattttctgcctgtggacagcccctcaaatgtcaataggtgcgcccctcatctgtcagcactctgcccctcaagtgtcaaggatcgcgcccctcatctgtcagtagtcgcgcccctcaagtgtcaataccgcagggcacttatccccaggcttgtccacatcatctgtgggaaactcgcgtaaaatcaggcgttttcgccgatttgcgaggctggccagctccacgtcgccggccgaaatcgagcctgcccctcatctgtcaacgccgcgccgggtgagtcggcccctcaagtgtcaacgtccgcccctcatctgtcagtgagggccaagttttccgcgaggtatccacaacgccggcggccggccgcggtgtctcgcacacggcttcgacggcgtttctggcgcgtttgcagggccatagacggccgccagcccagcggcgagggcaaccagccgagggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K524203_sequence/1
type: Sequence
persistent identity: BBa K524203 sequence
display id: BBa_K524203_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatgacgcatcctcacgataatatccgggtaggcgcaatcactttcgtctactccgttacaaagcgaggctgggtatttcccggcctttctgttatccgaaatccactgaaagcacagcggctggctgaggagataaataataaacgaggggctgtatgcacaaagcatcttctgttgagttaagaacgagtatcgagatggcacatagccttgctcaaattggaatcaggtttgtgccaataccagtagaaacagacgaagaatccatgggtatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattagtagtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcggttgcggccgcaatcgggcaaatcgctgaatcctgcattatgtcgtgattgagcgggatgaaaagtacatggcgagttttgacgagatcgtgccggagttcatcgaaaaaatggacgaggcactggctgaaattggttttgtatttggggagcaatggcgctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K320000_sequence/1
type: Sequence
persistent identity: BBa K320000 sequence
display id: BBa_K320000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accttgggcaaggccgaaggccgcgcagtcgatcaacaagccccggaggggccactttttgccggagggggagccgcgccgaaggcgtgggggaaccccgcaggggtgcccttctttgggcaccaaagaactagatatagggcgaaatgcgaaagacttaaaaatcaacaacttaaaaaaggggggtacgcaacagctcattgcggcaccccccgcaatagctcattgcgtaggttaaagaaaatctgtaattgactgccacttttacgcaacgcataattgttgtcgcgctgccgaaaagttgcagctgattgcgcatggtgccgcaaccgtgcggcaccctaccgcatggagataagcatggccacgcagtccagagaaatcggcattcaagccaagaacaagcccggtcactgggtgcaaacggaacgcaaagcgcatgaggcgtgggccgggcttattgcgaggaaacccacggcggcaatgctgctgcatcacctcgtggcgcagatgggccaccagaacgccgtggtggtcagccagaagacactttccaagctcatcggacgttctttgcggacggtccaatacgcagtcaaggacttggtggccgagcgctggatctccgtcgtgaagctcaacggccccggcaccgtgtcggcctacgtggtcaatgaccgcgtggcgtggggccagccccgcgaccagttgcgcctgtcggtgttcagtgccgccgtggtggttgatcacgacgaccaggacgaatcgctgttggggcatggcgacctgcgccgcatcccgaccctgtatccgggcgagcagcaactaccgaccggccccggcgaggagccgcccagccagcccggcattccgggcatggaaccagacctgccagccttgaccgaaacggaggaatgggaacggcgcgggcagcagcgcctgccgatgcccgatgagccgtgttttctggacgatggcgagccgttggagccgccgacacgggtcacgctgccgcgccggtagcacttgggttgcgcagcaacccgtaagtgcgctgttccagactatcggctgtagccgcctcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccgtttttatcaggctctgggaggcagaataaatgatcatatcgtcaattattacctccacggggagagcctgagcaaactggcctcaggcatttgagaagcacacggtcacactgcttccggtagtcaataaaccggtaaaccagcaatagacataagcggctatttaacgaccctgccctgaaccgacgaccgggtcgaattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50032_sequence/1
type: Sequence
persistent identity: BBa I50032 sequence
display id: BBa_I50032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0055_sequence/1
type: Sequence
persistent identity: BBa B0055 sequence
display id: BBa_B0055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0044_sequence/1
type: Sequence
persistent identity: BBa B0044 sequence
display id: BBa_B0044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagtcactaaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0042_sequence/1
type: Sequence
persistent identity: BBa B0042 sequence
display id: BBa_B0042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagttagttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K320004_sequence/1
type: Sequence
persistent identity: BBa K320004 sequence
display id: BBa_K320004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagagaccttgggcaaggccgaaggccgcgcagtcgatcaacaagccccggaggggccactttttgccggagggggagccgcgccgaaggcgtgggggaaccccgcaggggtgcccttctttgggcaccaaagaactagatatagggcgaaatgcgaaagacttaaaaatcaacaacttaaaaaaggggggtacgcaacagctcattgcggcaccccccgcaatagctcattgcgtaggttaaagaaaatctgtaattgactgccacttttacgcaacgcataattgttgtcgcgctgccgaaaagttgcagctgattgcgcatggtgccgcaaccgtgcggcaccctaccgcatggagataagcatggccacgcagtccagagaaatcggcattcaagccaagaacaagcccggtcactgggtgcaaacggaacgcaaagcgcatgaggcgtgggccgggcttattgcgaggaaacccacggcggcaatgctgctgcatcacctcgtggcgcagatgggccaccagaacgccgtggtggtcagccagaagacactttccaagctcatcggacgttctttgcggacggtccaatacgcagtcaaggacttggtggccgagcgctggatctccgtcgtgaagctcaacggccccggcaccgtgtcggcctacgtggtcaatgaccgcgtggcgtggggccagccccgcgaccagttgcgcctgtcggtgttcagtgccgccgtggtggttgatcacgacgaccaggacgaatcgctgttggggcatggcgacctgcgccgcatcccgaccctgtatccgggcgagcagcaactaccgaccggccccggcgaggagccgcccagccagcccggcattccgggcatggaaccagacctgccagccttgaccgaaacggaggaatgggaacggcgcgggcagcagcgcctgccgatgcccgatgagccgtgttttctggacgatggcgagccgttggagccgccgacacgggtcacgctgccgcgccggtagcacttgggttgcgcagcaacccgtaagtgcgctgttccagactatcggctgtagccgcctcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccgtttttatcaggctctgggaggcagaataaatgatcatatcgtcaattattacctccacggggagagcctgagcaaactggcctcaggcatttgagaagcacacggtcacactgcttccggtagtcaataaaccggtaaaccagcaatagacataagcggctatttaacgaccctgccctgaaccgacgaccgggtcgaattttactagagctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtctt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50050_sequence/1
type: Sequence
persistent identity: BBa I50050 sequence
display id: BBa_I50050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atccctggcttgttgtccacaaccgttaaaccttaaaagctttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagccatgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagtacgtactatcaacaggttgaactgctgatcttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50042_sequence/1
type: Sequence
persistent identity: BBa I50042 sequence
display id: BBa_I50042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K864051_sequence/1
type: Sequence
persistent identity: BBa K864051 sequence
display id: BBa_K864051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggtctagtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgttcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaacagatgaacagcatgtaacacctaatagaacaggtgaaaccagtaaaacaaagcaactagaacatgaaattgaacacctgagacaacttgttacagctca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125510_sequence/1
type: Sequence
persistent identity: BBa K125510 sequence
display id: BBa_K125510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatgaagaacgacaggactttgcaggccataggccgacagctcaaggccatgggctgtgagcgcttcgatatcggcgtcagggacgcacccaccggccagatgatgaaccgggaatggtcagccgccgaagtgctccagaacacgccatggctcaagcggatgaatgcccagggcaatgacgtgtatatcaggcccgccgagcaggagcggcatggtctggtgctggtggacgacctcagcgagtttgacctggatgacatgaaagccgagggccgggagcctgccctggtagtggaaaccagcccgaagaactatcaggcatgggtcaaggtggccgacgccgcaggcggtgaacttcgggggcagattgcccggacgctggccagcgagtacgacgccgacccggccagcgccgacagccgccactatggccgcttggcgggcttcaccaaccgcaaggacaagcacaccacccgcgccggttatcagccgtgggtgctgctgcgtgaatccaagggcaagaccgccaccgctggcccggcgctggtgcagcaggctggccagcagatcgagcaggcccagcggcagcaggagaaggcccgcaggctggccagcctcgaactgcccgagcggcagcttagccgccaccggcgcacggcgctggacgagtaccgcagcgagatggccgggctggtcaagcgcttcggtcatgacctcagcaagtgcgactttatcgccgcgcagaagctggccagccggggccgcagtgccgaggaaatcggcaaggccatggccgaggccagcccagcgctggcagagcgcaagcccggccacgaagcggattacatcgagcgcaccgtcagcaaggtcatgggtctgcccagcgtccagcttgcgcgggccgagctggcacgggcaccggcaccccgccagcgaggcatggacaggggcgggccagatttcagcatgtagtgcttgcgttggtactcacgcctgttatactatgagtactcacgcacagaagggggttttatggaatacgaaaaaagcgcttcagggtcggtctacctgatcaaaagtgacaagggctattggttgcccggtggctttggttatacgtcaaacaaggccgaggctggccgcttttcagtcgctgatatggccagccttaaccttgacggctgcaccttgtccttgttccgcgaagacaagcctttcggccccggcaagtttctcggtgactgatatgaaagaccaaaaggacaagcagaccggcgacctgctggccagccctgacgctgtacgccaagcgcgatatgccgagcgcatgaaggccaaagggatgcgtcagcgcaagttctggctgaccgacgacgaatacgaggcgctgcgcgagtgcctggaagaactcagagcggcgcagggcgggggtagtgaccccgccagcgcctaaccaccaactgcctgcaaaggaggcaatcaatggctacccataagcctatcaatattctggaggcgttcgcagcagcgccgccaccgctggactacgttttgcccaacatggtggccggtacggtcggggcgctggtgtcgcccggtggtgccggtaaatccatgctggccctgcaactggccgcacagattgcaggcgggccggatctgctggaggtgggcgaactgcccaccggcccggtgatctacctgcccgccgaagacccgcccaccgccattcatcaccgcctgcacgcccttggggcgcacctcagcgccgaggaacggcaagccgtggctgacggcctgctgatccagccgctgatcggcagcctgcccaacatcatggccccggagtggttcgacggcctcaagcgcgccgccgagggccgccgcctgatggtgctggacacgctgcgccggttccacatcgaggaagaaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K864050_sequence/1
type: Sequence
persistent identity: BBa K864050 sequence
display id: BBa_K864050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggtctagtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaacagatgaacagcatgtaacacctaatagaacaggtgaaaccagtaaaacaaagcaactagaacatgaaattgaacacctgagacaacttgttacagctca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50041_sequence/1
type: Sequence
persistent identity: BBa I50041 sequence
display id: BBa_I50041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttacattgtcgatctgttcatggtgaacagctttgaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50030_sequence/1
type: Sequence
persistent identity: BBa I50030 sequence
display id: BBa_I50030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggccaggacccaacgctgcccgagatgcgccgcgtgcggctgctggagatggcggacgcgatggatatgttctgccaagggttggtttgcgcattcacagttctccgcaagaattgattggctccaattcttggagtggtgaatccgttagcgagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125500_sequence/1
type: Sequence
persistent identity: BBa K125500 sequence
display id: BBa_K125500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K558005_sequence/1
type: Sequence
persistent identity: BBa K558005 sequence
display id: BBa_K558005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttaaaggaggttaaaatgtctagtgaaagcagccagggcctggtgacccgcctggccctgatcgctgctatcggtggtctgctgtttggctacgatagcgccgtgatcgccgccatcggtaccccagtggatatccactttatcgctccccgccacctgagcgctaccgccgccgccagcctgagcggtatggtggtggtggctgtgctggtcggctgcgtgaccggcagcctgctgagcggttggatcggcatccgctttggccgccgcggtggtctgctgatgagcagcatctgctttgtggccgccggctttggtgctgccctgaccgaaaaactgtttggtaccggtggtagcgctctgcaaatcttttgctttttccgctttctggccggcctgggcatcggcgtcgtgagcaccctgacccccacctacatcgccgaaatcgccccccccgataaacgcggtcagatggtcagcggccagcagatggctatcgtgaccggtgccctgaccggttacatctttacctggctgctggcccactttggcagcatcgattgggtgaacgccagcggttggtgctggagcccagccagcgaaggtctgatcggtatcgcctttctgctgctgctgctgaccgctccagataccccccactggctggtgatgaaaggccgccacagcgaagccagcaaaatcctggcccgcctggaaccccaggccgatcccaacctgaccatccagaaaatcaaagccggctttgataaagccatggataaaagcagcgccggcctgtttgcctttggcatcaccgtggtgtttgccggcgtgagcgtggccgcctttcagcagctggtcggcatcaacgccgtgctgtactacgccccccagatgtttcagaacctgggctttggcgccgataccgccctgctgcaaaccatcagcatcggcgtggtgaactttatctttaccatgatcgccagccgcgtggtggatcgctttggtcgcaaaccactgctgatctggggtgccctgggcatggctgctatgatggctgtgctgggctgctgcttttggtttaaagtgggcggcgtgctgcccctggccagcgtgctgctgtacatcgccgtgtttggcatgagctggggccccgtgtgctgggtggtgctgagcgaaatgtttcccagcagcatcaaaggcgccgccatgcccatcgccgtgaccggccagtggctggccaacatcctggtgaactttctgtttaaagtggccgatggcagccccgccctgaaccagacctttaaccacggctttagctacctggtgtttgccgccctgagcatcctgggcggcctgatcgtggcccgctttgtgcccgaaaccaaaggccgcagcctggatgaaatcgaagaaatgtggcgcagccagaaaggcagcagccaccaccaccaccaccactaataatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0045_sequence/1
type: Sequence
persistent identity: BBa B0045 sequence
display id: BBa_B0045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863201_sequence/1
type: Sequence
persistent identity: BBa K863201 sequence
display id: BBa_K863201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgagtatctatgattggaagtatgggaatggtgatacccgcattcttcagtgtcttgaggtctcctatcagattatgcccaactaaagcaaccggaggaggagatttcatggtaaatttctctgacttttggtcatcagtagactcgaactgtgagactatctcggttatgacagcagaaatgtccttcttggagacagtaaatgaagtcccaccaataaagaaatccttgttatcaggaacaaacttcttgtttcgaactttttcggtgccttgaactataaaatgtagagtggatatgtcgggtaggaatggagcgggcaaatgcttaccttctggaccttcaagaggtatgtagggtttgtagatactgatgccaacttcagtgacaacgttgctatttcgttcaaaccattccgaatccagagaaatcaaagttgtttgtctactattgatccaagccagtgcggtcttgaaactgacaatagtgtgctcgtgttttgaggtcatctttgtatgaataaatctagtctttgatctaaataatcttgacgagccagacgataataccaatctaaactctttaaacgttaaaggacaagtatgtctgcctgtattaaaccccaaatcagctcgtagtctgatcctcatcaacttgaggggcactatcttgtttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0062_sequence/1
type: Sequence
persistent identity: BBa B0062 sequence
display id: BBa_B0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagataaaaaaaatccttagctttcgctaaggatgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50001_sequence/1
type: Sequence
persistent identity: BBa I50001 sequence
display id: BBa_I50001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acttgcatcggatgcagcccggtgaacgtgccggcacggcctgggtaaccaggtattttgtccacataaccgtgcgcaaaatgttgtggataagcaggacacagcagcaatccacagcaggcatacaaccgcacaccgaggttactccgttctacaggttacgacgacatgtcaatacttgcccttgacaggcattgatggaatcgtagtctcacgctgatagtctgatcgacaatacaagtgggaccgtggtcccagaccgataatcagaccgacaacacgagtgggatcgtggtcccagactaataatcagaccgacgatacgagtgggaccgtggtcccagactaataatcagaccgacgatacgagtgggaccgtggttccagactaataatcagaccgacgatacgagtgggaccgtggtcccagactaataatcagaccgacgatacgagtgggaccatggtcccagactaataatcagaccgacgatacgagtgggaccgtggtcccagtctgattatcagaccgacgatacgagtgggaccgtggtcccagactaataatcagaccgacgatacgagtgggaccgtggtcccagactaataatcagaccgacgatacgagtgggaccgtggtcccagtctgattatcagaccgacgatacaagtggaacagtgggcccagagagaatattcaggccagttatgctttctggcctgtaacaaaggacattaagtaaagacagataaacgtagactaaaacgtggtcgcatcagggtgctggcttttcaagttccttaagaatggcctcaattttctctatacactcagttggaacacgagacctgtccaggttaagcaccattttatcgcccttatacaatactgtcgctccaggagcaaactgatgtcgtgagcttaaacttgttcttgatgcagatgacgttttaagcacagaagttaaaagagtgataacttcttcagcttcaaatatcaccccagcttttttctgctcatgaaggttagatgcctgctgcttaagtaattcctctttatctgtaaaggctttttgaagtgcatcacctgaccgggcagatagttcaccggggtgagaaaaaagagcaacaactgatttaggcaatttggcggtgttgatacagcgggtaataatcttacgtgaaatattttccgcatcagccagcgcagaaatatttccagcaaattcattctgcaatcggcttgcataacgctgaccacgttcataagcacttgttgggcgataatcgttacccaatctggataatgcagccatctgctcatcatccagctcgccaaccagaacacgataatcactttcggtaagtgcagcagctttacgacggcgactcccatcggcaatttctatgacaccagatactcttcgaccgaacgccggtgtctgttgaccagtcagtagaaaagaagggatgagatcatccagtgcgtcctcagtaagcagctcctggtcacgttcattacctgaccatacccgagaggtcttctcaacactatcaccccggagcacttcaagagtaaacttcacatcccgaccacatacaggcaaagtaatggcattaccgcgagccattactcctacgcgcgcaattaacgaatccaccatcggggcagctggtgtcgataacgaagtatcttcaaccggttgagtattgagcgtatgttttggaataacaggcgcacgcttcattatctaatctcccagcgtggtttaatcagacgatcgaaaatttcattgcagacaggttcccaaatagaaagagcatttctccaggcaccagttgaagagcgttgatcaatggcctgttcaaaaacagttctcatccggatctgacctttaccaacttcatccgtttcacgtacaacattttttagaaccatgcttccccaggcatcccgaatttgctcctccatccacggggactgagagccattactattg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61001_sequence/1
type: Sequence
persistent identity: BBa J61001 sequence
display id: BBa_J61001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgattcgcacgggcccatggctaattcccatgtcagccgttaagtgttcctgtgtcactcaaaattgctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagctttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagccatgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagtacgtactatcaacaggttgaactgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125320_sequence/1
type: Sequence
persistent identity: BBa K125320 sequence
display id: BBa_K125320_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50000_sequence/1
type: Sequence
persistent identity: BBa I50000 sequence
display id: BBa_I50000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgagtgagcgaggaagcaccagggaacagcacttatatattctgcttacacacgatgcctgaaaaaacttcccttggggttatccacttatccacggggatatttttataattattttttttatagtttttagatcttcttttttagagcgccttgtaggcctttatccatgctggttctacagaaggtgttgtgacaaattgccctttcagtgtgacaaatcaccctcaaatgacagtcctgtctgtgacaaattgcccttaaccctgtgacaaattgccctcagaagaagctgttttttcacaaagttatccctgcttattgactcttttttatttagtgtgacaatctaaaaacttgtcacacttcacatggatctgtcatggcggaaacagcggttatcaatcacaagaaacgtaaaaatagcccgcgaatcgtccagtcaaacgacctcactgaggcggcatatagtctctcccgggatcaaaaacgtatgctgtatctgttcgttgaccagatcagaaaatctgatggcaccctacaggaacatgacggtatctgcgagatccatgttgctaaatatgctgaaatattcggattgacctctgcggaagccagtaaggatatacggcaggcattgaagagtttcgcggggaaggaagtggttttttatcgccctgaagaggatgccggcgatgaaaaaggctatgaatcttttccttggtttatcaaacgtgcgcacagtccatccagagggctttacagtgtacatatcaacccatatctcattcccttctttatcgggttacagaaccggtttacgcagtttcggcttagtgaaacaaaagaaatcaccaatccgtatgccatgcgtttatacgaatccctgtgtcagtatcgtaagccggatggctcaggcatcgtctctctgaaaatcgactggatcatagagcgttaccagctgcctcaaagttaccagcgtatgcctgacttccgccgccgcttcctgcaagtctgtgttaatgagatcaacagcagaactccaatgcgcctctcatacattgagaaaaagaaaggccgccagacgactcatatcgtattttccttccgcgatatcacttccatgacgacaggatagtctgagggttatctgtcacagatttgagggtggttcgtcacatttgttctgacctactgagggtaatttgtcacagttttgctgtttccttcagcctgcatggattttctcatactttttgaactgtaatttttaaggaagccaaatttgagggcagtttgtcacagttgatttccttctctttcccttcgtcatgtgacttgatatcgggggttagttcgtcatcattgatgagggttgattatcacagtttattactctgaattggctatccgcgtgtgtacctctacctggagtttttcccacggtggatatttcttcttgcgctgagcgtaagagctatctgacagaacagttcttctttgcttcctcgccagttcgctcgctatgctcggttacacggctgcggcgagcgctagtgataataagtgactgaggtatgtgctcttcttatctccttttgtagtgttgctcttattttaaacaactttgcggttttttgatgactttgcgattttgttgttgctttgcagtaaattgcaagatttaataaaaaaacgcaaagcaatgattaaaggatgttcagaatgaaactcatggaaacacttaaccagtgcataaacgctggtcatgaaatgacgaaggctatcgccattgcacagtttaatgatgacagcccggaagcgaggaaaataacccggcgctggagaataggtgaagcagcggatttagttggggtttcttctcaggctatcagagatgccgagaaagcagggcgactaccgcacccggatatggaaattcgaggacgggttgagcaacgtgttggttatacaattgaacaaattaatcatatgcgtg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125020_sequence/1
type: Sequence
persistent identity: BBa K125020 sequence
display id: BBa_K125020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatgaagaacgacaggactttgcaggccataggccgacagctcaaggccatgggctgtgagcgcttcgatatcggcgtcagggacgcacccaccggccagatgatgaaccgggaatggtcagccgccgaagtgctccagaacacgccatggctcaagcggatgaatgcccagggcaatgacgtgtatatcaggcccgccgagcaggagcggcatggtctggtgctggtggacgacctcagcgagtttgacctggatgacatgaaagccgagggccgggagcctgccctggtagtggaaaccagcccgaagaactatcaggcatgggtcaaggtggccgacgccgcaggcggtgaacttcgggggcagattgcccggacgctggccagcgagtacgacgccgacccggccagcgccgacagccgccactatggccgcttggcgggcttcaccaaccgcaaggacaagcacaccacccgcgccggttatcagccgtgggtgctgctgcgtgaatccaagggcaagaccgccaccgctggcccggcgctggtgcagcaggctggccagcagatcgagcaggcccagcggcagcaggagaaggcccgcaggctggccagcctcgaactgcccgagcggcagcttagccgccaccggcgcacggcgctggacgagtaccgcagcgagatggccgggctggtcaagcgcttcggtcatgacctcagcaagtgcgactttatcgccgcgcagaagctggccagccggggccgcagtgccgaggaaatcggcaaggccatggccgaggccagcccagcgctggcagagcgcaagcccggccacgaagcggattacatcgagcgcaccgtcagcaaggtcatgggtctgcccagcgtccagcttgcgcgggccgagctggcacgggcaccggcaccccgccagcgaggcatggacaggggcgggccagatttcagcatgtagtgcttgcgttggtactcacgcctgttatactatgagtactcacgcacagaagggggttttatggaatacgaaaaaagcgcttcagggtcggtctacctgatcaaaagtgacaagggctattggttgcccggtggctttggttatacgtcaaacaaggccgaggctggccgcttttcagtcgctgatatggccagccttaaccttgacggctgcaccttgtccttgttccgcgaagacaagcctttcggccccggcaagtttctcggtgactgatatgaaagaccaaaaggacaagcagaccggcgacctgctggccagccctgacgctgtacgccaagcgcgatatgccgagcgcatgaaggccaaagggatgcgtcagcgcaagttctggctgaccgacgacgaatacgaggcgctgcgcgagtgcctggaagaactcagagcggcgcagggcgggggtagtgaccccgccagcgcctaaccaccaactgcctgcaaaggaggcaatcaatggctacccataagcctatcaatattctggaggcgttcgcagcagcgccgccaccgctggactacgttttgcccaacatggtggccggtacggtcggggcgctggtgtcgcccggtggtgccggtaaatccatgctggccctgcaactggccgcacagattgcaggcgggccggatctgctggaggtgggcgaactgcccaccggcccggtgatctacctgcccgccgaagacccgcccaccgccattcatcaccgcctgcacgcccttggggcgcacctcagcgccgaggaacggcaagccgtggctgacggcctgctgatccagccgctgatcggcagcctgcccaacatcatggccccggagtggttcgacggcctcaagcgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50040_sequence/1
type: Sequence
persistent identity: BBa I50040 sequence
display id: BBa_I50040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacagtaagacgggtaagcctgttgatgataccgctgccttactggctgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctaaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgattcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctctattggtatagtgattaaaatcacctagactaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccaatggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaggagatattgccttatcttttccagttaaaaaagttcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgagtttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggacctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacgtaacggactaagaaaaacactacacgatgctttaactgcaaaaatccagctcaccagttttgaggcaaagtttttgagtgacatgcaaagtaagcatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatttaaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttagatatcaaagggaaaactgtccaaatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcatttaaagctgttcaccatgaacagatcgacaatgtaacagatgaacagcatgtaacacctaatagaacaggtgaaaccagtaaaacaaagcaactagaacatgaaattgaacacctgagacaacttgttacagctca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14032_sequence/1
type: Sequence
persistent identity: BBa I14032 sequence
display id: BBa_I14032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50052_sequence/1
type: Sequence
persistent identity: BBa I50052 sequence
display id: BBa_I50052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgttcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00000_sequence/1
type: Sequence
persistent identity: BBa G00000 sequence
display id: BBa_G00000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00001_sequence/1
type: Sequence
persistent identity: BBa G00001 sequence
display id: BBa_G00001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K653001_sequence/1
type: Sequence
persistent identity: BBa K653001 sequence
display id: BBa_K653001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0043_sequence/1
type: Sequence
persistent identity: BBa B0043 sequence
display id: BBa_B0043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttagtgactc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00102_sequence/1
type: Sequence
persistent identity: BBa G00102 sequence
display id: BBa_G00102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcactcaaaggcggtaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z