//plasmid/component/origin
type: Collection
persistent identity: plasmid component origin
display id: plasmid_component_origin
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: pSC101ts
member: p15A
member: Ori+Kan
member: F plasmid
member: BBa_I50030
member: BBa_K533000
member: pSC101
member: BBa_K112125
member: B-H-R plas
member: pSC101 ts
member: pUC19 ori
member: R6K
member: Ori
member: BBa_I50050
member: OriV
member: BBa_I50001
member: BBa_K864050
member: BBa_K524203

pSC101
type: ComponentDefinition
persistent identity: BBa I50040
display id: BBa_I50040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nonfunctional pSC101 origin of replication
creator: Reshma Shetty
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: direct repeat
sequence annotation: inverted repeat
sequence annotation: promoter region
sequence annotation: A->T mutation to remove MfeI site
sequence annotation: potential hairpin
sequence annotation: 11bp sequence repeated 4 times in oriC
sequence annotation: RBS
sequence annotation: par region
sequence annotation: repA
sequence annotation: AT rich region
sequence annotation: -10 signal functional promoter
sequence annotation: T->A mutation to remove MfeI site
sequence: https://synbiohub.org/public/igem/BBa_I50040_sequence/1

direct repeat
type: SequenceAnnotation
persistent identity: annotation1796227
display id: annotation1796227
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796227/range1796227/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796227/range1796227/1
type: Range
persistent identity: range1796227
display id: range1796227
version: 1
direction: direction/forward
top level: pSC101
start: 603
end: 620
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1796229
display id: annotation1796229
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796229/range1796229/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796229/range1796229/1
type: Range
persistent identity: range1796229
display id: range1796229
version: 1
direction: direction/forward
top level: pSC101
start: 702
end: 723
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation1796230
display id: annotation1796230
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796230/range1796230/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796230/range1796230/1
type: Range
persistent identity: range1796230
display id: range1796230
version: 1
direction: direction/forward
top level: pSC101
start: 710
end: 729
orientation: inline

A->T mutation to remove MfeI site
type: SequenceAnnotation
persistent identity: annotation1880623
display id: annotation1880623
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1880623/range1880623/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1880623/range1880623/1
type: Range
persistent identity: range1880623
display id: range1880623
version: 1
direction: direction/forward
top level: pSC101
start: 708
end: 708
orientation: inline

potential hairpin
type: SequenceAnnotation
persistent identity: annotation1796237
display id: annotation1796237
version: 1
top level: pSC101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796237/range1796237/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796237/range1796237/1
type: Range
persistent identity: range1796237
display id: range1796237
version: 1
direction: direction/forward
top level: pSC101
start: 1608
end: 1682
orientation: inline

potential hairpin
type: SequenceAnnotation
persistent identity: annotation1796238
display id: annotation1796238
version: 1
top level: pSC101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796238/range1796238/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796238/range1796238/1
type: Range
persistent identity: range1796238
display id: range1796238
version: 1
direction: direction/forward
top level: pSC101
start: 2063
end: 2088
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1796228
display id: annotation1796228
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796228/range1796228/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796228/range1796228/1
type: Range
persistent identity: range1796228
display id: range1796228
version: 1
direction: direction/forward
top level: pSC101
start: 631
end: 654
orientation: inline

11bp sequence repeated 4 times in oriC
type: SequenceAnnotation
persistent identity: annotation1796224
display id: annotation1796224
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796224/range1796224/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796224/range1796224/1
type: Range
persistent identity: range1796224
display id: range1796224
version: 1
direction: direction/forward
top level: pSC101
start: 482
end: 492
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1796233
display id: annotation1796233
version: 1
top level: pSC101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796233/range1796233/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796233/range1796233/1
type: Range
persistent identity: range1796233
display id: range1796233
version: 1
direction: direction/forward
top level: pSC101
start: 734
end: 746
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1796226
display id: annotation1796226
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796226/range1796226/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796226/range1796226/1
type: Range
persistent identity: range1796226
display id: range1796226
version: 1
direction: direction/forward
top level: pSC101
start: 578
end: 601
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1796223
display id: annotation1796223
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796223/range1796223/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796223/range1796223/1
type: Range
persistent identity: range1796223
display id: range1796223
version: 1
direction: direction/reverse
top level: pSC101
start: 191
end: 201
orientation: inline

potential hairpin
type: SequenceAnnotation
persistent identity: annotation1796231
display id: annotation1796231
version: 1
top level: pSC101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796231/range1796231/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796231/range1796231/1
type: Range
persistent identity: range1796231
display id: range1796231
version: 1
direction: direction/forward
top level: pSC101
start: 683
end: 761
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation1796222
display id: annotation1796222
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796222/range1796222/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796222/range1796222/1
type: Range
persistent identity: range1796222
display id: range1796222
version: 1
direction: direction/forward
top level: pSC101
start: 1
end: 368
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation1796236
display id: annotation1796236
version: 1
top level: pSC101
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796236/range1796236/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796236/range1796236/1
type: Range
persistent identity: range1796236
display id: range1796236
version: 1
direction: direction/forward
top level: pSC101
start: 750
end: 1700
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation1796225
display id: annotation1796225
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796225/range1796225/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796225/range1796225/1
type: Range
persistent identity: range1796225
display id: range1796225
version: 1
direction: direction/forward
top level: pSC101
start: 503
end: 581
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1796234
display id: annotation1796234
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796234/range1796234/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796234/range1796234/1
type: Range
persistent identity: range1796234
display id: range1796234
version: 1
direction: direction/reverse
top level: pSC101
start: 726
end: 749
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1796221
display id: annotation1796221
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796221/range1796221/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796221/range1796221/1
type: Range
persistent identity: range1796221
display id: range1796221
version: 1
direction: direction/forward
top level: pSC101
start: 180
end: 189
orientation: inline

-10 signal functional promoter
type: SequenceAnnotation
persistent identity: annotation1796235
display id: annotation1796235
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796235/range1796235/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796235/range1796235/1
type: Range
persistent identity: range1796235
display id: range1796235
version: 1
direction: direction/forward
top level: pSC101
start: 948
end: 953
orientation: inline

-10 signal functional promoter
type: SequenceAnnotation
persistent identity: annotation1796232
display id: annotation1796232
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1796232/range1796232/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1796232/range1796232/1
type: Range
persistent identity: range1796232
display id: range1796232
version: 1
direction: direction/forward
top level: pSC101
start: 723
end: 727
orientation: inline

T->A mutation to remove MfeI site
type: SequenceAnnotation
persistent identity: annotation1880624
display id: annotation1880624
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50040/annotation1880624/range1880624/1

https://synbiohub.org/public/igem/BBa_I50040/annotation1880624/range1880624/1
type: Range
persistent identity: range1880624
display id: range1880624
version: 1
direction: direction/forward
top level: pSC101
start: 742
end: 742
orientation: inline

BBa_I50030
type: ComponentDefinition
persistent identity: BBa I50030
display id: BBa_I50030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBR322 replication origin
creator: Reshma Shetty
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the replication origin from plasmid pBR322 which is supposed to have low to medium copy number.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: rep pMB1
sequence annotation: RNAI transcript
sequence annotation: repeat region found in Tn3
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: replication origin
sequence annotation: RBS 2
sequence annotation: RNAI transcript promoter -10 sequence
sequence annotation: from pSC101
sequence annotation: gamma-delta insertion target sequence
sequence annotation: H-strand Y effector site
sequence annotation: RNAI transcript promoter -35 sequence
sequence annotation: L-strand Y effector site
sequence annotation: pMB1 origin of replication
sequence annotation: RBS 1
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: RNAII transcript
sequence: https://synbiohub.org/public/igem/BBa_I50030_sequence/1

rep pMB1
type: SequenceAnnotation
persistent identity: annotation1796201
display id: annotation1796201
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796201/range1796201/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796201/range1796201/1
type: Range
persistent identity: range1796201
display id: range1796201
version: 1
direction: direction/forward
top level: BBa_I50030
start: 160
end: 774
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1796198
display id: annotation1796198
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796198/range1796198/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796198/range1796198/1
type: Range
persistent identity: range1796198
display id: range1796198
version: 1
direction: direction/reverse
top level: BBa_I50030
start: 210
end: 317
orientation: inline

repeat region found in Tn3
type: SequenceAnnotation
persistent identity: annotation1796195
display id: annotation1796195
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796195/range1796195/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796195/range1796195/1
type: Range
persistent identity: range1796195
display id: range1796195
version: 1
direction: direction/reverse
top level: BBa_I50030
start: 108
end: 145
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1796197
display id: annotation1796197
version: 1
top level: BBa_I50030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796197/range1796197/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796197/range1796197/1
type: Range
persistent identity: range1796197
display id: range1796197
version: 1
direction: direction/forward
top level: BBa_I50030
start: 192
end: 197
orientation: inline

replication origin
type: SequenceAnnotation
persistent identity: annotation1796203
display id: annotation1796203
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796203/range1796203/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796203/range1796203/1
type: Range
persistent identity: range1796203
display id: range1796203
version: 1
direction: direction/reverse
top level: BBa_I50030
start: 758
end: 758
orientation: inline

RBS 2
type: SequenceAnnotation
persistent identity: annotation1796208
display id: annotation1796208
version: 1
top level: BBa_I50030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796208/range1796208/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796208/range1796208/1
type: Range
persistent identity: range1796208
display id: range1796208
version: 1
direction: direction/reverse
top level: BBa_I50030
start: 1384
end: 1388
orientation: inline

RNAI transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1796199
display id: annotation1796199
version: 1
top level: BBa_I50030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796199/range1796199/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796199/range1796199/1
type: Range
persistent identity: range1796199
display id: range1796199
version: 1
direction: direction/reverse
top level: BBa_I50030
start: 326
end: 331
orientation: inline

from pSC101
type: SequenceAnnotation
persistent identity: annotation1796210
display id: annotation1796210
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796210/range1796210/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796210/range1796210/1
type: Range
persistent identity: range1796210
display id: range1796210
version: 1
direction: direction/reverse
top level: BBa_I50030
start: 1531
end: 1657
orientation: inline

gamma-delta insertion target sequence
type: SequenceAnnotation
persistent identity: annotation1796211
display id: annotation1796211
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796211/range1796211/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796211/range1796211/1
type: Range
persistent identity: range1796211
display id: range1796211
version: 1
direction: direction/forward
top level: BBa_I50030
start: 1774
end: 1778
orientation: inline

H-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1796206
display id: annotation1796206
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796206/range1796206/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796206/range1796206/1
type: Range
persistent identity: range1796206
display id: range1796206
version: 1
direction: direction/reverse
top level: BBa_I50030
start: 1126
end: 1282
orientation: inline

RNAI transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1796200
display id: annotation1796200
version: 1
top level: BBa_I50030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796200/range1796200/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796200/range1796200/1
type: Range
persistent identity: range1796200
display id: range1796200
version: 1
direction: direction/reverse
top level: BBa_I50030
start: 348
end: 353
orientation: inline

gamma-delta insertion target sequence
type: SequenceAnnotation
persistent identity: annotation1796209
display id: annotation1796209
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796209/range1796209/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796209/range1796209/1
type: Range
persistent identity: range1796209
display id: range1796209
version: 1
direction: direction/forward
top level: BBa_I50030
start: 1501
end: 1505
orientation: inline

L-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1796204
display id: annotation1796204
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796204/range1796204/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796204/range1796204/1
type: Range
persistent identity: range1796204
display id: range1796204
version: 1
direction: direction/forward
top level: BBa_I50030
start: 879
end: 942
orientation: inline

pMB1 origin of replication
type: SequenceAnnotation
persistent identity: annotation1796194
display id: annotation1796194
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796194/range1796194/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796194/range1796194/1
type: Range
persistent identity: range1796194
display id: range1796194
version: 1
direction: direction/forward
top level: BBa_I50030
start: 171
end: 759
orientation: inline

RBS 1
type: SequenceAnnotation
persistent identity: annotation1796207
display id: annotation1796207
version: 1
top level: BBa_I50030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796207/range1796207/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796207/range1796207/1
type: Range
persistent identity: range1796207
display id: range1796207
version: 1
direction: direction/reverse
top level: BBa_I50030
start: 1383
end: 1388
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1796196
display id: annotation1796196
version: 1
top level: BBa_I50030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796196/range1796196/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796196/range1796196/1
type: Range
persistent identity: range1796196
display id: range1796196
version: 1
direction: direction/forward
top level: BBa_I50030
start: 171
end: 176
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1796202
display id: annotation1796202
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796202/range1796202/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796202/range1796202/1
type: Range
persistent identity: range1796202
display id: range1796202
version: 1
direction: direction/forward
top level: BBa_I50030
start: 207
end: 759
orientation: inline

gamma-delta insertion target sequence
type: SequenceAnnotation
persistent identity: annotation1796205
display id: annotation1796205
version: 1
top level: BBa_I50030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50030/annotation1796205/range1796205/1

https://synbiohub.org/public/igem/BBa_I50030/annotation1796205/range1796205/1
type: Range
persistent identity: range1796205
display id: range1796205
version: 1
direction: direction/forward
top level: BBa_I50030
start: 1044
end: 1048
orientation: inline

pSC101ts
type: ComponentDefinition
persistent identity: BBa K864051
display id: BBa_K864051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSC101ts temperature sensitive low copy origin of replication
creator: Erik Lundin
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C.
mutable notes: -
mutable provenance: pSIM5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Tandem repeat
sequence annotation: Repeat structure
sequence annotation: Repeated sequence
sequence annotation: Hairpin structure
sequence annotation: pSC101ts ori
sequence annotation: Ins(GGC)
sequence annotation: A>T mutation to remove SpeI
sequence annotation: Repeat "4"
sequence annotation: -35 signal
sequence annotation: repA
sequence annotation: par region
sequence annotation: AT rich region
sequence annotation: Repeat "5"
sequence annotation: A56V ts mutation
sequence: https://synbiohub.org/public/igem/BBa_K864051_sequence/1

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202701
display id: annotation2202701
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202701/range2202701/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202701/range2202701/1
type: Range
persistent identity: range2202701
display id: range2202701
version: 1
direction: direction/forward
top level: pSC101ts
start: 631
end: 652
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202697
display id: annotation2202697
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202697/range2202697/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202697/range2202697/1
type: Range
persistent identity: range2202697
display id: range2202697
version: 1
direction: direction/forward
top level: pSC101ts
start: 578
end: 599
orientation: inline

Repeat structure
type: SequenceAnnotation
persistent identity: annotation2202693
display id: annotation2202693
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202693/range2202693/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202693/range2202693/1
type: Range
persistent identity: range2202693
display id: range2202693
version: 1
direction: direction/forward
top level: pSC101ts
start: 190
end: 204
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202698
display id: annotation2202698
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202698/range2202698/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202698/range2202698/1
type: Range
persistent identity: range2202698
display id: range2202698
version: 1
direction: direction/reverse
top level: pSC101ts
start: 582
end: 589
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202700
display id: annotation2202700
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202700/range2202700/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202700/range2202700/1
type: Range
persistent identity: range2202700
display id: range2202700
version: 1
direction: direction/reverse
top level: pSC101ts
start: 603
end: 610
orientation: inline

Hairpin structure
type: SequenceAnnotation
persistent identity: annotation2202707
display id: annotation2202707
version: 1
top level: pSC101ts
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202707/range2202707/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202707/range2202707/1
type: Range
persistent identity: range2202707
display id: range2202707
version: 1
direction: direction/forward
top level: pSC101ts
start: 683
end: 761
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202711
display id: annotation2202711
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202711/range2202711/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202711/range2202711/1
type: Range
persistent identity: range2202711
display id: range2202711
version: 1
direction: direction/reverse
top level: pSC101ts
start: 712
end: 719
orientation: inline

Hairpin structure
type: SequenceAnnotation
persistent identity: annotation2202712
display id: annotation2202712
version: 1
top level: pSC101ts
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202712/range2202712/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202712/range2202712/1
type: Range
persistent identity: range2202712
display id: range2202712
version: 1
direction: direction/forward
top level: pSC101ts
start: 1608
end: 1682
orientation: inline

Repeat structure
type: SequenceAnnotation
persistent identity: annotation2202695
display id: annotation2202695
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202695/range2202695/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202695/range2202695/1
type: Range
persistent identity: range2202695
display id: range2202695
version: 1
direction: direction/forward
top level: pSC101ts
start: 232
end: 247
orientation: inline

pSC101ts ori
type: SequenceAnnotation
persistent identity: annotation2202690
display id: annotation2202690
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202690/range2202690/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202690/range2202690/1
type: Range
persistent identity: range2202690
display id: range2202690
version: 1
direction: direction/forward
top level: pSC101ts
start: 1
end: 2216
orientation: inline

Ins(GGC)
type: SequenceAnnotation
persistent identity: annotation2202713
display id: annotation2202713
version: 1
top level: pSC101ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202713/range2202713/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202713/range2202713/1
type: Range
persistent identity: range2202713
display id: range2202713
version: 1
direction: direction/forward
top level: pSC101ts
start: 2141
end: 2143
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202702
display id: annotation2202702
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202702/range2202702/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202702/range2202702/1
type: Range
persistent identity: range2202702
display id: range2202702
version: 1
direction: direction/reverse
top level: pSC101ts
start: 635
end: 642
orientation: inline

A>T mutation to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2202706
display id: annotation2202706
version: 1
top level: pSC101ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202706/range2202706/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202706/range2202706/1
type: Range
persistent identity: range2202706
display id: range2202706
version: 1
direction: direction/forward
top level: pSC101ts
start: 678
end: 678
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2202703
display id: annotation2202703
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202703/range2202703/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202703/range2202703/1
type: Range
persistent identity: range2202703
display id: range2202703
version: 1
direction: direction/forward
top level: pSC101ts
start: 671
end: 693
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202699
display id: annotation2202699
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202699/range2202699/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202699/range2202699/1
type: Range
persistent identity: range2202699
display id: range2202699
version: 1
direction: direction/forward
top level: pSC101ts
start: 601
end: 620
orientation: inline

-35 signal
type: SequenceAnnotation
persistent identity: annotation2202708
display id: annotation2202708
version: 1
top level: pSC101ts
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202708/range2202708/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202708/range2202708/1
type: Range
persistent identity: range2202708
display id: range2202708
version: 1
direction: direction/forward
top level: pSC101ts
start: 692
end: 697
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2202709
display id: annotation2202709
version: 1
top level: pSC101ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202709/range2202709/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202709/range2202709/1
type: Range
persistent identity: range2202709
display id: range2202709
version: 1
direction: direction/forward
top level: pSC101ts
start: 750
end: 1700
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202705
display id: annotation2202705
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202705/range2202705/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202705/range2202705/1
type: Range
persistent identity: range2202705
display id: range2202705
version: 1
direction: direction/reverse
top level: pSC101ts
start: 676
end: 683
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation2202692
display id: annotation2202692
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202692/range2202692/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202692/range2202692/1
type: Range
persistent identity: range2202692
display id: range2202692
version: 1
direction: direction/forward
top level: pSC101ts
start: 1
end: 368
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation2202696
display id: annotation2202696
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202696/range2202696/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202696/range2202696/1
type: Range
persistent identity: range2202696
display id: range2202696
version: 1
direction: direction/forward
top level: pSC101ts
start: 499
end: 581
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2202710
display id: annotation2202710
version: 1
top level: pSC101ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202710/range2202710/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202710/range2202710/1
type: Range
persistent identity: range2202710
display id: range2202710
version: 1
direction: direction/forward
top level: pSC101ts
start: 702
end: 723
orientation: inline

A56V ts mutation
type: SequenceAnnotation
persistent identity: annotation2202688
display id: annotation2202688
version: 1
top level: pSC101ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864051/annotation2202688/range2202688/1

https://synbiohub.org/public/igem/BBa_K864051/annotation2202688/range2202688/1
type: Range
persistent identity: range2202688
display id: range2202688
version: 1
direction: direction/forward
top level: pSC101ts
start: 915
end: 917
orientation: inline

pSC101
type: ComponentDefinition
persistent identity: BBa I50042
display id: BBa_I50042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSC101 origin of replication
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids.
mutable notes: The replication origin boundaries were chosen quite liberally (i.e. they might include more sequence than is strictly necessary) because a different version of this origin (BBa_I50040) was shown to be non-functional.
mutable provenance: This part was amplified from pSB4A3-P1010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: putative polyA
sequence annotation: direct repeat
sequence annotation: inverted repeat
sequence annotation: AT rich region
sequence annotation: RBS
sequence annotation: par region
sequence annotation: -10
sequence annotation: G to A difference with canonical pSC101 vector sequence
sequence annotation: Terminator
sequence annotation: repA
sequence annotation: Tn3 integration site
sequence annotation: G to C difference with canonical pSC101 vector sequence
sequence annotation: T to C difference with canonical pSC101 vector sequence
sequence annotation: promoter region
sequence annotation: mutated SpeI site
sequence annotation: -35
sequence annotation: C to T difference with canonical pSC101 vector sequence
sequence: https://synbiohub.org/public/igem/BBa_I50042_sequence/1

putative polyA
type: SequenceAnnotation
persistent identity: annotation2071333
display id: annotation2071333
version: 1
top level: pSC101
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071333/range2071333/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071333/range2071333/1
type: Range
persistent identity: range2071333
display id: range2071333
version: 1
direction: direction/forward
top level: pSC101
start: 1947
end: 1962
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071319
display id: annotation2071319
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071319/range2071319/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071319/range2071319/1
type: Range
persistent identity: range2071319
display id: range2071319
version: 1
direction: direction/forward
top level: pSC101
start: 769
end: 788
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071317
display id: annotation2071317
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071317/range2071317/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071317/range2071317/1
type: Range
persistent identity: range2071317
display id: range2071317
version: 1
direction: direction/forward
top level: pSC101
start: 716
end: 735
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071322
display id: annotation2071322
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071322/range2071322/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071322/range2071322/1
type: Range
persistent identity: range2071322
display id: range2071322
version: 1
direction: direction/forward
top level: pSC101
start: 838
end: 859
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation2071316
display id: annotation2071316
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071316/range2071316/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071316/range2071316/1
type: Range
persistent identity: range2071316
display id: range2071316
version: 1
direction: direction/forward
top level: pSC101
start: 635
end: 717
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2071326
display id: annotation2071326
version: 1
top level: pSC101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071326/range2071326/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071326/range2071326/1
type: Range
persistent identity: range2071326
display id: range2071326
version: 1
direction: direction/forward
top level: pSC101
start: 870
end: 882
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071314
display id: annotation2071314
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071314/range2071314/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071314/range2071314/1
type: Range
persistent identity: range2071314
display id: range2071314
version: 1
direction: direction/forward
top level: pSC101
start: 192
end: 215
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation2071310
display id: annotation2071310
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071310/range2071310/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071310/range2071310/1
type: Range
persistent identity: range2071310
display id: range2071310
version: 1
direction: direction/forward
top level: pSC101
start: 137
end: 504
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2071324
display id: annotation2071324
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071324/range2071324/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071324/range2071324/1
type: Range
persistent identity: range2071324
display id: range2071324
version: 1
direction: direction/forward
top level: pSC101
start: 850
end: 855
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071325
display id: annotation2071325
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071325/range2071325/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071325/range2071325/1
type: Range
persistent identity: range2071325
display id: range2071325
version: 1
direction: direction/forward
top level: pSC101
start: 862
end: 884
orientation: inline

G to A difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071329
display id: annotation2071329
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071329/range2071329/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071329/range2071329/1
type: Range
persistent identity: range2071329
display id: range2071329
version: 1
direction: direction/forward
top level: pSC101
start: 1655
end: 1655
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071315
display id: annotation2071315
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071315/range2071315/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071315/range2071315/1
type: Range
persistent identity: range2071315
display id: range2071315
version: 1
direction: direction/forward
top level: pSC101
start: 221
end: 238
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071311
display id: annotation2071311
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071311/range2071311/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071311/range2071311/1
type: Range
persistent identity: range2071311
display id: range2071311
version: 1
direction: direction/forward
top level: pSC101
start: 139
end: 152
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2071309
display id: annotation2071309
version: 1
top level: pSC101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071309/range2071309/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071309/range2071309/1
type: Range
persistent identity: range2071309
display id: range2071309
version: 1
direction: direction/forward
top level: pSC101
start: 46
end: 75
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071334
display id: annotation2071334
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071334/range2071334/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071334/range2071334/1
type: Range
persistent identity: range2071334
display id: range2071334
version: 1
direction: direction/forward
top level: pSC101
start: 1963
end: 1979
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2071328
display id: annotation2071328
version: 1
top level: pSC101
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071328/range2071328/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071328/range2071328/1
type: Range
persistent identity: range2071328
display id: range2071328
version: 1
direction: direction/forward
top level: pSC101
start: 886
end: 1836
orientation: inline

Tn3 integration site
type: SequenceAnnotation
persistent identity: annotation2071312
display id: annotation2071312
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071312/range2071312/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071312/range2071312/1
type: Range
persistent identity: range2071312
display id: range2071312
version: 1
direction: direction/forward
top level: pSC101
start: 158
end: 162
orientation: inline

G to C difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071332
display id: annotation2071332
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071332/range2071332/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071332/range2071332/1
type: Range
persistent identity: range2071332
display id: range2071332
version: 1
direction: direction/forward
top level: pSC101
start: 1912
end: 1912
orientation: inline

T to C difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071335
display id: annotation2071335
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071335/range2071335/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071335/range2071335/1
type: Range
persistent identity: range2071335
display id: range2071335
version: 1
direction: direction/forward
top level: pSC101
start: 2002
end: 2002
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation2071323
display id: annotation2071323
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071323/range2071323/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071323/range2071323/1
type: Range
persistent identity: range2071323
display id: range2071323
version: 1
direction: direction/forward
top level: pSC101
start: 846
end: 865
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071318
display id: annotation2071318
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071318/range2071318/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071318/range2071318/1
type: Range
persistent identity: range2071318
display id: range2071318
version: 1
direction: direction/forward
top level: pSC101
start: 737
end: 756
orientation: inline

mutated SpeI site
type: SequenceAnnotation
persistent identity: annotation2071320
display id: annotation2071320
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071320/range2071320/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071320/range2071320/1
type: Range
persistent identity: range2071320
display id: range2071320
version: 1
direction: direction/reverse
top level: pSC101
start: 814
end: 819
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071313
display id: annotation2071313
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071313/range2071313/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071313/range2071313/1
type: Range
persistent identity: range2071313
display id: range2071313
version: 1
direction: direction/forward
top level: pSC101
start: 165
end: 181
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071331
display id: annotation2071331
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071331/range2071331/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071331/range2071331/1
type: Range
persistent identity: range2071331
display id: range2071331
version: 1
direction: direction/forward
top level: pSC101
start: 1847
end: 1861
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2071321
display id: annotation2071321
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071321/range2071321/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071321/range2071321/1
type: Range
persistent identity: range2071321
display id: range2071321
version: 1
direction: direction/forward
top level: pSC101
start: 828
end: 833
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2071327
display id: annotation2071327
version: 1
top level: pSC101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071327/range2071327/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071327/range2071327/1
type: Range
persistent identity: range2071327
display id: range2071327
version: 1
direction: direction/forward
top level: pSC101
start: 879
end: 884
orientation: inline

C to T difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071330
display id: annotation2071330
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071330/range2071330/1

https://synbiohub.org/public/igem/BBa_I50042/annotation2071330/range2071330/1
type: Range
persistent identity: range2071330
display id: range2071330
version: 1
direction: direction/forward
top level: pSC101
start: 1750
end: 1750
orientation: inline

B-H-R plas
type: ComponentDefinition
persistent identity: BBa K125020
display id: BBa_K125020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lac promoter/rbs/rep/oriV/lac promoter/rbs/aadA/oriT
creator: Margaret Ruzicka
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an intermediate construction step for the construction of the broad-host-range plasmid derived from RSF1010. The coding region was inserted into pSB1A3.
mutable notes: I am having trouble inserting this part into the Base Vector so the construct will be tested while still in pSB1A3.
mutable provenance: This part was derived from RSF1010 and RP4.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_K125320
component: BBa_I14032
component: BBa_B0034
component: BBa_K125310
component: BBa_K125340
component: BBa_K125500
component: BBa_K125510
sequence annotation: BBa_K125320
sequence annotation: BBa_I14032
sequence annotation: BBa_K125310
sequence annotation: BBa_K125340
sequence annotation: BBa_B0034
sequence annotation: BBa_K125510
sequence annotation: BBa_K125500
sequence: https://synbiohub.org/public/igem/BBa_K125020_sequence/1

BBa_K125320
type: Component
persistent identity: component1992600
display id: component1992600
version: 1
top level: B-H-R plas
access: public
definition: https://synbiohub.org/public/igem/BBa_K125320/1

BBa_I14032
type: Component
persistent identity: component1992593
display id: component1992593
version: 1
top level: B-H-R plas
access: public
definition: https://synbiohub.org/public/igem/BBa_I14032/1

BBa_B0034
type: Component
persistent identity: component1992595
display id: component1992595
version: 1
top level: B-H-R plas
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K125310
type: Component
persistent identity: component1992597
display id: component1992597
version: 1
top level: B-H-R plas
access: public
definition: https://synbiohub.org/public/igem/BBa_K125310/1

BBa_B0034
type: Component
persistent identity: component1992578
display id: component1992578
version: 1
top level: B-H-R plas
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I14032
type: Component
persistent identity: component1992576
display id: component1992576
version: 1
top level: B-H-R plas
access: public
definition: https://synbiohub.org/public/igem/BBa_I14032/1

BBa_K125340
type: Component
persistent identity: component1992589
display id: component1992589
version: 1
top level: B-H-R plas
access: public
definition: OriV

BBa_K125500
type: Component
persistent identity: component1992599
display id: component1992599
version: 1
top level: B-H-R plas
access: public
definition: https://synbiohub.org/public/igem/BBa_K125500/1

BBa_K125510
type: Component
persistent identity: component1992585
display id: component1992585
version: 1
top level: B-H-R plas
access: public
definition: https://synbiohub.org/public/igem/BBa_K125510/1

BBa_K125320
type: SequenceAnnotation
persistent identity: annotation1992600
display id: annotation1992600
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992600/range1992600/1
component: BBa_K125320

https://synbiohub.org/public/igem/BBa_K125020/annotation1992600/range1992600/1
type: Range
persistent identity: range1992600
display id: range1992600
version: 1
direction: direction/reverse
top level: B-H-R plas
start: 4743
end: 4841
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1992593
display id: annotation1992593
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992593/range1992593/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125020/annotation1992593/range1992593/1
type: Range
persistent identity: range1992593
display id: range1992593
version: 1
direction: direction/reverse
top level: B-H-R plas
start: 3891
end: 3927
orientation: inline

BBa_K125310
type: SequenceAnnotation
persistent identity: annotation1992597
display id: annotation1992597
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992597/range1992597/1
component: BBa_K125310

https://synbiohub.org/public/igem/BBa_K125020/annotation1992597/range1992597/1
type: Range
persistent identity: range1992597
display id: range1992597
version: 1
direction: direction/reverse
top level: B-H-R plas
start: 3956
end: 4008
orientation: inline

BBa_K125340
type: SequenceAnnotation
persistent identity: annotation1992589
display id: annotation1992589
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992589/range1992589/1
component: BBa_K125340

https://synbiohub.org/public/igem/BBa_K125020/annotation1992589/range1992589/1
type: Range
persistent identity: range1992589
display id: range1992589
version: 1
direction: direction/reverse
top level: B-H-R plas
start: 3459
end: 3882
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1992595
display id: annotation1992595
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992595/range1992595/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125020/annotation1992595/range1992595/1
type: Range
persistent identity: range1992595
display id: range1992595
version: 1
direction: direction/reverse
top level: B-H-R plas
start: 3936
end: 3947
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1992576
display id: annotation1992576
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992576/range1992576/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125020/annotation1992576/range1992576/1
type: Range
persistent identity: range1992576
display id: range1992576
version: 1
direction: direction/reverse
top level: B-H-R plas
start: 1
end: 37
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1992578
display id: annotation1992578
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992578/range1992578/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125020/annotation1992578/range1992578/1
type: Range
persistent identity: range1992578
display id: range1992578
version: 1
direction: direction/reverse
top level: B-H-R plas
start: 46
end: 57
orientation: inline

BBa_K125510
type: SequenceAnnotation
persistent identity: annotation1992585
display id: annotation1992585
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992585/range1992585/1
component: BBa_K125510

https://synbiohub.org/public/igem/BBa_K125020/annotation1992585/range1992585/1
type: Range
persistent identity: range1992585
display id: range1992585
version: 1
direction: direction/reverse
top level: B-H-R plas
start: 66
end: 3450
orientation: inline

BBa_K125500
type: SequenceAnnotation
persistent identity: annotation1992599
display id: annotation1992599
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992599/range1992599/1
component: BBa_K125500

https://synbiohub.org/public/igem/BBa_K125020/annotation1992599/range1992599/1
type: Range
persistent identity: range1992599
display id: range1992599
version: 1
direction: direction/reverse
top level: B-H-R plas
start: 4017
end: 4734
orientation: inline

pUC19 ori
type: ComponentDefinition
persistent identity: BBa I50022
display id: BBa_I50022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Minimal pUC19-derived high copy replication origin
creator: Reshma Shetty
created: 2008-01-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites. Lacks mutations that render BBa_I50020 nonfunctional as a replication origin.
mutable notes: We had to leave several restriction sites in because point mutations that eliminated these sites were found to render the origin nonfunctional.
mutable provenance: pSB1A3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: ORI
sequence annotation: origin of replication
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: RNAI transcript promoter -10
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: RNAII transcript
sequence annotation: rep (pMB1)
sequence annotation: RNAI transcript promoter -35
sequence annotation: RNAI transcript
sequence: https://synbiohub.org/public/igem/BBa_I50022_sequence/1

ORI
type: SequenceAnnotation
persistent identity: annotation1959125
display id: annotation1959125
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959125/range1959125/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959125/range1959125/1
type: Range
persistent identity: range1959125
display id: range1959125
version: 1
direction: direction/reverse
top level: pUC19 ori
start: 615
end: 615
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1959119
display id: annotation1959119
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959119/range1959119/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959119/range1959119/1
type: Range
persistent identity: range1959119
display id: range1959119
version: 1
direction: direction/forward
top level: pUC19 ori
start: 27
end: 615
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1959120
display id: annotation1959120
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959120/range1959120/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959120/range1959120/1
type: Range
persistent identity: range1959120
display id: range1959120
version: 1
direction: direction/forward
top level: pUC19 ori
start: 48
end: 53
orientation: inline

RNAI transcript promoter -10
type: SequenceAnnotation
persistent identity: annotation1959123
display id: annotation1959123
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959123/range1959123/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959123/range1959123/1
type: Range
persistent identity: range1959123
display id: range1959123
version: 1
direction: direction/reverse
top level: pUC19 ori
start: 182
end: 187
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1959118
display id: annotation1959118
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959118/range1959118/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959118/range1959118/1
type: Range
persistent identity: range1959118
display id: range1959118
version: 1
direction: direction/forward
top level: pUC19 ori
start: 27
end: 32
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1959121
display id: annotation1959121
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959121/range1959121/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959121/range1959121/1
type: Range
persistent identity: range1959121
display id: range1959121
version: 1
direction: direction/forward
top level: pUC19 ori
start: 63
end: 615
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1959117
display id: annotation1959117
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959117/range1959117/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959117/range1959117/1
type: Range
persistent identity: range1959117
display id: range1959117
version: 1
direction: direction/forward
top level: pUC19 ori
start: 16
end: 630
orientation: inline

RNAI transcript promoter -35
type: SequenceAnnotation
persistent identity: annotation1959124
display id: annotation1959124
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959124/range1959124/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959124/range1959124/1
type: Range
persistent identity: range1959124
display id: range1959124
version: 1
direction: direction/reverse
top level: pUC19 ori
start: 204
end: 209
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1959122
display id: annotation1959122
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959122/range1959122/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959122/range1959122/1
type: Range
persistent identity: range1959122
display id: range1959122
version: 1
direction: direction/reverse
top level: pUC19 ori
start: 66
end: 173
orientation: inline

BBa_I50050
type: ComponentDefinition
persistent identity: BBa I50050
display id: BBa_I50050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R6K replication origin
creator: Reshma Shetty
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R6K minimal origin of replication. Only contains gamma origin and requires pir+ strain for replication. Copy number is dependent on amount of pir protein in the cell.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: R6K origin
sequence annotation: gamma origin
sequence: https://synbiohub.org/public/igem/BBa_I50050_sequence/1

R6K origin
type: SequenceAnnotation
persistent identity: annotation1796245
display id: annotation1796245
version: 1
top level: BBa_I50050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50050/annotation1796245/range1796245/1

https://synbiohub.org/public/igem/BBa_I50050/annotation1796245/range1796245/1
type: Range
persistent identity: range1796245
display id: range1796245
version: 1
direction: direction/forward
top level: BBa_I50050
start: 1
end: 306
orientation: inline

gamma origin
type: SequenceAnnotation
persistent identity: annotation1796246
display id: annotation1796246
version: 1
top level: BBa_I50050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50050/annotation1796246/range1796246/1

https://synbiohub.org/public/igem/BBa_I50050/annotation1796246/range1796246/1
type: Range
persistent identity: range1796246
display id: range1796246
version: 1
direction: direction/forward
top level: BBa_I50050
start: 37
end: 312
orientation: inline

pSC101
type: ComponentDefinition
persistent identity: BBa I50041
display id: BBa_I50041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSC101 origin of replication, reverse
creator: Reshma Shetty
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. This part is the reverse copy of BBa_I50040.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: repA
sequence annotation: RBS
sequence annotation: promoter region
sequence annotation: putative polyA
sequence annotation: difference from canonical pSC101 sequence in Genbank
sequence annotation: inverted repeat
sequence annotation: Tn3 integration site
sequence annotation: terminator
sequence annotation: par region
sequence annotation: rep promoter -10
sequence annotation: direct repeat
sequence annotation: AT rich region
sequence annotation: rep promoter -35
sequence annotation: Mutated SpeI site
sequence: https://synbiohub.org/public/igem/BBa_I50041_sequence/1

repA
type: SequenceAnnotation
persistent identity: annotation1932216
display id: annotation1932216
version: 1
top level: pSC101
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932216/range1932216/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932216/range1932216/1
type: Range
persistent identity: range1932216
display id: range1932216
version: 1
direction: direction/reverse
top level: pSC101
start: 202
end: 1152
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1932219
display id: annotation1932219
version: 1
top level: pSC101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932219/range1932219/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932219/range1932219/1
type: Range
persistent identity: range1932219
display id: range1932219
version: 1
direction: direction/reverse
top level: pSC101
start: 1156
end: 1168
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation1932220
display id: annotation1932220
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932220/range1932220/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932220/range1932220/1
type: Range
persistent identity: range1932220
display id: range1932220
version: 1
direction: direction/reverse
top level: pSC101
start: 1173
end: 1192
orientation: inline

putative polyA
type: SequenceAnnotation
persistent identity: annotation1932215
display id: annotation1932215
version: 1
top level: pSC101
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932215/range1932215/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932215/range1932215/1
type: Range
persistent identity: range1932215
display id: range1932215
version: 1
direction: direction/reverse
top level: pSC101
start: 76
end: 91
orientation: inline

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080857
display id: annotation2080857
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080857/range2080857/1

https://synbiohub.org/public/igem/BBa_I50041/annotation2080857/range2080857/1
type: Range
persistent identity: range2080857
display id: range2080857
version: 1
direction: direction/forward
top level: pSC101
start: 126
end: 126
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932230
display id: annotation1932230
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932230/range1932230/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932230/range1932230/1
type: Range
persistent identity: range1932230
display id: range1932230
version: 1
direction: direction/reverse
top level: pSC101
start: 1800
end: 1817
orientation: inline

Tn3 integration site
type: SequenceAnnotation
persistent identity: annotation1932233
display id: annotation1932233
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932233/range1932233/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932233/range1932233/1
type: Range
persistent identity: range1932233
display id: range1932233
version: 1
direction: direction/reverse
top level: pSC101
start: 1876
end: 1880
orientation: inline

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080859
display id: annotation2080859
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080859/range2080859/1

https://synbiohub.org/public/igem/BBa_I50041/annotation2080859/range2080859/1
type: Range
persistent identity: range2080859
display id: range2080859
version: 1
direction: direction/forward
top level: pSC101
start: 383
end: 383
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1932235
display id: annotation1932235
version: 1
top level: pSC101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932235/range1932235/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932235/range1932235/1
type: Range
persistent identity: range1932235
display id: range1932235
version: 1
direction: direction/reverse
top level: pSC101
start: 1963
end: 1992
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation1932229
display id: annotation1932229
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932229/range1932229/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932229/range1932229/1
type: Range
persistent identity: range1932229
display id: range1932229
version: 1
direction: direction/reverse
top level: pSC101
start: 1534
end: 1901
orientation: inline

rep promoter -10
type: SequenceAnnotation
persistent identity: annotation1932222
display id: annotation1932222
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932222/range1932222/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932222/range1932222/1
type: Range
persistent identity: range1932222
display id: range1932222
version: 1
direction: direction/reverse
top level: pSC101
start: 1183
end: 1188
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1932225
display id: annotation1932225
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932225/range1932225/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932225/range1932225/1
type: Range
persistent identity: range1932225
display id: range1932225
version: 1
direction: direction/reverse
top level: pSC101
start: 1250
end: 1269
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation1932228
display id: annotation1932228
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932228/range1932228/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932228/range1932228/1
type: Range
persistent identity: range1932228
display id: range1932228
version: 1
direction: direction/reverse
top level: pSC101
start: 1321
end: 1403
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1932227
display id: annotation1932227
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932227/range1932227/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932227/range1932227/1
type: Range
persistent identity: range1932227
display id: range1932227
version: 1
direction: direction/reverse
top level: pSC101
start: 1303
end: 1322
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932217
display id: annotation1932217
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932217/range1932217/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932217/range1932217/1
type: Range
persistent identity: range1932217
display id: range1932217
version: 1
direction: direction/reverse
top level: pSC101
start: 1154
end: 1176
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932231
display id: annotation1932231
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932231/range1932231/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932231/range1932231/1
type: Range
persistent identity: range1932231
display id: range1932231
version: 1
direction: direction/reverse
top level: pSC101
start: 1823
end: 1846
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1932218
display id: annotation1932218
version: 1
top level: pSC101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932218/range1932218/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932218/range1932218/1
type: Range
persistent identity: range1932218
display id: range1932218
version: 1
direction: direction/reverse
top level: pSC101
start: 1154
end: 1159
orientation: inline

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080856
display id: annotation2080856
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080856/range2080856/1

https://synbiohub.org/public/igem/BBa_I50041/annotation2080856/range2080856/1
type: Range
persistent identity: range2080856
display id: range2080856
version: 1
direction: direction/forward
top level: pSC101
start: 36
end: 36
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932234
display id: annotation1932234
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932234/range1932234/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932234/range1932234/1
type: Range
persistent identity: range1932234
display id: range1932234
version: 1
direction: direction/reverse
top level: pSC101
start: 1886
end: 1899
orientation: inline

difference from canonical pSC101 sequence in Genbank
type: SequenceAnnotation
persistent identity: annotation2080858
display id: annotation2080858
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50041/annotation2080858/range2080858/1

https://synbiohub.org/public/igem/BBa_I50041/annotation2080858/range2080858/1
type: Range
persistent identity: range2080858
display id: range2080858
version: 1
direction: direction/forward
top level: pSC101
start: 288
end: 288
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932232
display id: annotation1932232
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932232/range1932232/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932232/range1932232/1
type: Range
persistent identity: range1932232
display id: range1932232
version: 1
direction: direction/reverse
top level: pSC101
start: 1857
end: 1873
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1932221
display id: annotation1932221
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932221/range1932221/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932221/range1932221/1
type: Range
persistent identity: range1932221
display id: range1932221
version: 1
direction: direction/reverse
top level: pSC101
start: 1179
end: 1200
orientation: inline

rep promoter -35
type: SequenceAnnotation
persistent identity: annotation1932223
display id: annotation1932223
version: 1
top level: pSC101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932223/range1932223/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932223/range1932223/1
type: Range
persistent identity: range1932223
display id: range1932223
version: 1
direction: direction/reverse
top level: pSC101
start: 1205
end: 1210
orientation: inline

Mutated SpeI site
type: SequenceAnnotation
persistent identity: annotation1932224
display id: annotation1932224
version: 1
top level: pSC101
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932224/range1932224/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932224/range1932224/1
type: Range
persistent identity: range1932224
display id: range1932224
version: 1
direction: direction/reverse
top level: pSC101
start: 1219
end: 1224
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1932226
display id: annotation1932226
version: 1
top level: pSC101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50041/annotation1932226/range1932226/1

https://synbiohub.org/public/igem/BBa_I50041/annotation1932226/range1932226/1
type: Range
persistent identity: range1932226
display id: range1932226
version: 1
direction: direction/reverse
top level: pSC101
start: 1282
end: 1301
orientation: inline

BBa_I50001
type: ComponentDefinition
persistent identity: BBa I50001
display id: BBa_I50001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F plasmid backbone with BioBrick sites removed, reverse
creator: Reshma Shetty
created: 2006-03-11T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a (near) single copy origin derived from the F plasmid. The naturally occurring BioBricks sites were removed with an intent to preserve the amino acid and codon frequency where possible. Similar to the pSMART VC sequence from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. It is intended to be combined with other vector components (antibiotic resistance marker, multiple cloning site) in order to form a BioBricks vector. Relevant information can be found at http://openwetware.org/wiki/BioBrick_Parts_for_Plasmid_Engineering. This part is a reverse copy of BBa_I50000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: C to T mutation (eliminates PstI site)
sequence annotation: parA
sequence annotation: iteron (repeat region)
sequence annotation: sopC (IncD) repeat region
sequence annotation: sequence derived from F plasmid in GenBank Accession Number AP001918
sequence annotation: DnaA box
sequence annotation: C to G mutation (eliminates XbaI site, noncoding region)
sequence annotation: repE
sequence annotation: parB
sequence annotation: A/T region
sequence annotation: ori2 (oriS) single copy replication origin
sequence annotation: A to T mutation (eliminates SpeI site)
sequence: https://synbiohub.org/public/igem/BBa_I50001_sequence/1

C to T mutation (eliminates PstI site)
type: SequenceAnnotation
persistent identity: annotation1820921
display id: annotation1820921
version: 1
top level: BBa_I50001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820921/range1820921/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820921/range1820921/1
type: Range
persistent identity: range1820921
display id: range1820921
version: 1
direction: direction/forward
top level: BBa_I50001
start: 3628
end: 3628
orientation: inline

parA
type: SequenceAnnotation
persistent identity: annotation1820918
display id: annotation1820918
version: 1
top level: BBa_I50001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820918/range1820918/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820918/range1820918/1
type: Range
persistent identity: range1820918
display id: range1820918
version: 1
direction: direction/reverse
top level: BBa_I50001
start: 1748
end: 2923
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1820924
display id: annotation1820924
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820924/range1820924/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820924/range1820924/1
type: Range
persistent identity: range1820924
display id: range1820924
version: 1
direction: direction/reverse
top level: BBa_I50001
start: 4376
end: 4395
orientation: inline

sopC (IncD) repeat region
type: SequenceAnnotation
persistent identity: annotation1820911
display id: annotation1820911
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820911/range1820911/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820911/range1820911/1
type: Range
persistent identity: range1820911
display id: range1820911
version: 1
direction: direction/forward
top level: BBa_I50001
start: 1
end: 703
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1820926
display id: annotation1820926
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820926/range1820926/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820926/range1820926/1
type: Range
persistent identity: range1820926
display id: range1820926
version: 1
direction: direction/reverse
top level: BBa_I50001
start: 4430
end: 4449
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1820925
display id: annotation1820925
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820925/range1820925/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820925/range1820925/1
type: Range
persistent identity: range1820925
display id: range1820925
version: 1
direction: direction/reverse
top level: BBa_I50001
start: 4409
end: 4428
orientation: inline

sequence derived from F plasmid in GenBank Accession Number AP001918
type: SequenceAnnotation
persistent identity: annotation1820919
display id: annotation1820919
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820919/range1820919/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820919/range1820919/1
type: Range
persistent identity: range1820919
display id: range1820919
version: 1
direction: direction/forward
top level: BBa_I50001
start: 1
end: 2921
orientation: inline

DnaA box
type: SequenceAnnotation
persistent identity: annotation1820931
display id: annotation1820931
version: 1
top level: BBa_I50001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820931/range1820931/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820931/range1820931/1
type: Range
persistent identity: range1820931
display id: range1820931
version: 1
direction: direction/reverse
top level: BBa_I50001
start: 4553
end: 4560
orientation: inline

DnaA box
type: SequenceAnnotation
persistent identity: annotation1820930
display id: annotation1820930
version: 1
top level: BBa_I50001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820930/range1820930/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820930/range1820930/1
type: Range
persistent identity: range1820930
display id: range1820930
version: 1
direction: direction/reverse
top level: BBa_I50001
start: 4544
end: 4552
orientation: inline

C to G mutation (eliminates XbaI site, noncoding region)
type: SequenceAnnotation
persistent identity: annotation1820927
display id: annotation1820927
version: 1
top level: BBa_I50001
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820927/range1820927/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820927/range1820927/1
type: Range
persistent identity: range1820927
display id: range1820927
version: 1
direction: direction/forward
top level: BBa_I50001
start: 4458
end: 4458
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1820923
display id: annotation1820923
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820923/range1820923/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820923/range1820923/1
type: Range
persistent identity: range1820923
display id: range1820923
version: 1
direction: direction/reverse
top level: BBa_I50001
start: 4354
end: 4373
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1820922
display id: annotation1820922
version: 1
top level: BBa_I50001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820922/range1820922/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820922/range1820922/1
type: Range
persistent identity: range1820922
display id: range1820922
version: 1
direction: direction/reverse
top level: BBa_I50001
start: 3502
end: 4257
orientation: inline

parB
type: SequenceAnnotation
persistent identity: annotation1820916
display id: annotation1820916
version: 1
top level: BBa_I50001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820916/range1820916/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820916/range1820916/1
type: Range
persistent identity: range1820916
display id: range1820916
version: 1
direction: direction/reverse
top level: BBa_I50001
start: 777
end: 1748
orientation: inline

A/T region
type: SequenceAnnotation
persistent identity: annotation1820929
display id: annotation1820929
version: 1
top level: BBa_I50001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820929/range1820929/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820929/range1820929/1
type: Range
persistent identity: range1820929
display id: range1820929
version: 1
direction: direction/reverse
top level: BBa_I50001
start: 4494
end: 4540
orientation: inline

ori2 (oriS) single copy replication origin
type: SequenceAnnotation
persistent identity: annotation1820928
display id: annotation1820928
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820928/range1820928/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820928/range1820928/1
type: Range
persistent identity: range1820928
display id: range1820928
version: 1
direction: direction/forward
top level: BBa_I50001
start: 4351
end: 4640
orientation: inline

C to T mutation (eliminates PstI site)
type: SequenceAnnotation
persistent identity: annotation1820917
display id: annotation1820917
version: 1
top level: BBa_I50001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820917/range1820917/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820917/range1820917/1
type: Range
persistent identity: range1820917
display id: range1820917
version: 1
direction: direction/forward
top level: BBa_I50001
start: 2088
end: 2088
orientation: inline

A to T mutation (eliminates SpeI site)
type: SequenceAnnotation
persistent identity: annotation1820915
display id: annotation1820915
version: 1
top level: BBa_I50001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820915/range1820915/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820915/range1820915/1
type: Range
persistent identity: range1820915
display id: range1820915
version: 1
direction: direction/forward
top level: BBa_I50001
start: 930
end: 930
orientation: inline

sequence derived from F plasmid in GenBank Accession Number AP001918
type: SequenceAnnotation
persistent identity: annotation1820920
display id: annotation1820920
version: 1
top level: BBa_I50001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50001/annotation1820920/range1820920/1

https://synbiohub.org/public/igem/BBa_I50001/annotation1820920/range1820920/1
type: Range
persistent identity: range1820920
display id: range1820920
version: 1
direction: direction/forward
top level: BBa_I50001
start: 3251
end: 4640
orientation: inline

BBa_K524203
type: ComponentDefinition
persistent identity: BBa K524203
display id: BBa_K524203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSC101 heat sensitive origin of replication with deletion in par locus
creator: HO Yuan Heng Trevor
created: 2012-08-25T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _689_
m user id: 0
owner id: 8758
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A derivative of pSC101 origin of replication, this heat sensitive origin has the par locus deleted, therefore allowing a rapid loss of plasmid even under the permissive temperature.
mutable notes: The par locus was deleted through excision of BBa_K524000 by SspI and StuI, purification of desired fragments and then
mutable provenance: Modified from BBa_K524000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Inverted Repeat 2 (IR2) ->
sequence annotation: DnaD protein binding sequence
sequence annotation: Direct Repeat 3 (DR3)
sequence annotation: -35 promoter region of repAts
sequence annotation: Inverted Repeat 2 (IR2) <-
sequence annotation: Direct Repeat 2 (DR2)
sequence annotation: -10 promoter region of repAts
sequence annotation: Direct Repeat 1 (DR1)
sequence annotation: Inverted Repeat 1 (IR1) ->
sequence annotation: Inverted Repeat 1 (IR1) <-
sequence annotation: IHF binding sequence
sequence annotation: scar of removed par locus
sequence annotation: repA101ts
sequence annotation: AT Rich Region
sequence: https://synbiohub.org/public/igem/BBa_K524203_sequence/1

Inverted Repeat 2 (IR2) ->
type: SequenceAnnotation
persistent identity: annotation2181022
display id: annotation2181022
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181022/range2181022/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181022/range2181022/1
type: Range
persistent identity: range2181022
display id: range2181022
version: 1
direction: direction/forward
top level: BBa_K524203
start: 1325
end: 1348
orientation: inline

DnaD protein binding sequence
type: SequenceAnnotation
persistent identity: annotation2181033
display id: annotation2181033
version: 1
top level: BBa_K524203
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181033/range2181033/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181033/range2181033/1
type: Range
persistent identity: range2181033
display id: range2181033
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 1582
end: 1590
orientation: inline

Direct Repeat 3 (DR3)
type: SequenceAnnotation
persistent identity: annotation2181028
display id: annotation2181028
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181028/range2181028/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181028/range2181028/1
type: Range
persistent identity: range2181028
display id: range2181028
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 1420
end: 1441
orientation: inline

-35 promoter region of repAts
type: SequenceAnnotation
persistent identity: annotation2181025
display id: annotation2181025
version: 1
top level: BBa_K524203
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181025/range2181025/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181025/range2181025/1
type: Range
persistent identity: range2181025
display id: range2181025
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 1376
end: 1381
orientation: inline

Inverted Repeat 2 (IR2) <-
type: SequenceAnnotation
persistent identity: annotation2181023
display id: annotation2181023
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181023/range2181023/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181023/range2181023/1
type: Range
persistent identity: range2181023
display id: range2181023
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 1349
end: 1371
orientation: inline

Direct Repeat 2 (DR2)
type: SequenceAnnotation
persistent identity: annotation2181029
display id: annotation2181029
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181029/range2181029/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181029/range2181029/1
type: Range
persistent identity: range2181029
display id: range2181029
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 1453
end: 1473
orientation: inline

-10 promoter region of repAts
type: SequenceAnnotation
persistent identity: annotation2181024
display id: annotation2181024
version: 1
top level: BBa_K524203
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181024/range2181024/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181024/range2181024/1
type: Range
persistent identity: range2181024
display id: range2181024
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 1354
end: 1359
orientation: inline

Direct Repeat 1 (DR1)
type: SequenceAnnotation
persistent identity: annotation2181030
display id: annotation2181030
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181030/range2181030/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181030/range2181030/1
type: Range
persistent identity: range2181030
display id: range2181030
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 1474
end: 1494
orientation: inline

Inverted Repeat 1 (IR1) ->
type: SequenceAnnotation
persistent identity: annotation2181026
display id: annotation2181026
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181026/range2181026/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181026/range2181026/1
type: Range
persistent identity: range2181026
display id: range2181026
version: 1
direction: direction/forward
top level: BBa_K524203
start: 1371
end: 1384
orientation: inline

Inverted Repeat 1 (IR1) <-
type: SequenceAnnotation
persistent identity: annotation2181027
display id: annotation2181027
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181027/range2181027/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181027/range2181027/1
type: Range
persistent identity: range2181027
display id: range2181027
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 1385
end: 1397
orientation: inline

IHF binding sequence
type: SequenceAnnotation
persistent identity: annotation2181031
display id: annotation2181031
version: 1
top level: BBa_K524203
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181031/range2181031/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181031/range2181031/1
type: Range
persistent identity: range2181031
display id: range2181031
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 1514
end: 1526
orientation: inline

scar of removed par locus
type: SequenceAnnotation
persistent identity: annotation2181034
display id: annotation2181034
version: 1
top level: BBa_K524203
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181034/range2181034/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181034/range2181034/1
type: Range
persistent identity: range2181034
display id: range2181034
version: 1
direction: direction/forward
top level: BBa_K524203
start: 1734
end: 1739
orientation: inline

repA101ts
type: SequenceAnnotation
persistent identity: annotation2181021
display id: annotation2181021
version: 1
top level: BBa_K524203
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181021/range2181021/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181021/range2181021/1
type: Range
persistent identity: range2181021
display id: range2181021
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 373
end: 1323
orientation: inline

AT Rich Region
type: SequenceAnnotation
persistent identity: annotation2181032
display id: annotation2181032
version: 1
top level: BBa_K524203
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K524203/annotation2181032/range2181032/1

https://synbiohub.org/public/igem/BBa_K524203/annotation2181032/range2181032/1
type: Range
persistent identity: range2181032
display id: range2181032
version: 1
direction: direction/reverse
top level: BBa_K524203
start: 1492
end: 1547
orientation: inline

BBa_K864050
type: ComponentDefinition
persistent identity: BBa K864050
display id: BBa_K864050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSC101 low copy origin of replication
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: BBa_K864050 is a pSC101 origin of replication and is found in the pSB4*15 series of BioBrick vectors. This ori is an improvement of the ori found in all pSB4*5 plasmids, BBa_I50042. BBa_I50042 has been shown to have a higher copy number than expected.
mutable notes: This ori was created by cloning pSC101ts from pSIM5 and making a V56A mutation in repA.
mutable provenance: pSIM5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Tandem repeat
sequence annotation: par region
sequence annotation: Repeated sequence
sequence annotation: Hairpin structure
sequence annotation: Repeat "4"
sequence annotation: AT rich region
sequence annotation: repA
sequence annotation: -35 signal
sequence annotation: pSC101 ori
sequence annotation: Ins(GGC)
sequence annotation: A>T mutation to remove SpeI
sequence annotation: Repeat structure
sequence annotation: Repeat "5"
sequence: https://synbiohub.org/public/igem/BBa_K864050_sequence/1

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202471
display id: annotation2202471
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202471/range2202471/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202471/range2202471/1
type: Range
persistent identity: range2202471
display id: range2202471
version: 1
direction: direction/forward
top level: BBa_K864050
start: 601
end: 620
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation2202464
display id: annotation2202464
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202464/range2202464/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202464/range2202464/1
type: Range
persistent identity: range2202464
display id: range2202464
version: 1
direction: direction/forward
top level: BBa_K864050
start: 1
end: 368
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202472
display id: annotation2202472
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202472/range2202472/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202472/range2202472/1
type: Range
persistent identity: range2202472
display id: range2202472
version: 1
direction: direction/reverse
top level: BBa_K864050
start: 603
end: 610
orientation: inline

Hairpin structure
type: SequenceAnnotation
persistent identity: annotation2202478
display id: annotation2202478
version: 1
top level: BBa_K864050
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202478/range2202478/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202478/range2202478/1
type: Range
persistent identity: range2202478
display id: range2202478
version: 1
direction: direction/forward
top level: BBa_K864050
start: 683
end: 761
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2202475
display id: annotation2202475
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202475/range2202475/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202475/range2202475/1
type: Range
persistent identity: range2202475
display id: range2202475
version: 1
direction: direction/forward
top level: BBa_K864050
start: 673
end: 691
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation2202468
display id: annotation2202468
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202468/range2202468/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202468/range2202468/1
type: Range
persistent identity: range2202468
display id: range2202468
version: 1
direction: direction/forward
top level: BBa_K864050
start: 499
end: 581
orientation: inline

Hairpin structure
type: SequenceAnnotation
persistent identity: annotation2202483
display id: annotation2202483
version: 1
top level: BBa_K864050
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202483/range2202483/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202483/range2202483/1
type: Range
persistent identity: range2202483
display id: range2202483
version: 1
direction: direction/forward
top level: BBa_K864050
start: 1608
end: 1682
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202474
display id: annotation2202474
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202474/range2202474/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202474/range2202474/1
type: Range
persistent identity: range2202474
display id: range2202474
version: 1
direction: direction/reverse
top level: BBa_K864050
start: 635
end: 642
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2202482
display id: annotation2202482
version: 1
top level: BBa_K864050
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202482/range2202482/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202482/range2202482/1
type: Range
persistent identity: range2202482
display id: range2202482
version: 1
direction: direction/forward
top level: BBa_K864050
start: 750
end: 1700
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202469
display id: annotation2202469
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202469/range2202469/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202469/range2202469/1
type: Range
persistent identity: range2202469
display id: range2202469
version: 1
direction: direction/forward
top level: BBa_K864050
start: 578
end: 599
orientation: inline

-35 signal
type: SequenceAnnotation
persistent identity: annotation2202479
display id: annotation2202479
version: 1
top level: BBa_K864050
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202479/range2202479/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202479/range2202479/1
type: Range
persistent identity: range2202479
display id: range2202479
version: 1
direction: direction/forward
top level: BBa_K864050
start: 692
end: 697
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2202465
display id: annotation2202465
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202465/range2202465/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202465/range2202465/1
type: Range
persistent identity: range2202465
display id: range2202465
version: 1
direction: direction/forward
top level: BBa_K864050
start: 1
end: 2216
orientation: inline

Ins(GGC)
type: SequenceAnnotation
persistent identity: annotation2202484
display id: annotation2202484
version: 1
top level: BBa_K864050
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202484/range2202484/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202484/range2202484/1
type: Range
persistent identity: range2202484
display id: range2202484
version: 1
direction: direction/forward
top level: BBa_K864050
start: 2141
end: 2143
orientation: inline

A>T mutation to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2202477
display id: annotation2202477
version: 1
top level: BBa_K864050
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202477/range2202477/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202477/range2202477/1
type: Range
persistent identity: range2202477
display id: range2202477
version: 1
direction: direction/forward
top level: BBa_K864050
start: 678
end: 678
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202481
display id: annotation2202481
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202481/range2202481/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202481/range2202481/1
type: Range
persistent identity: range2202481
display id: range2202481
version: 1
direction: direction/reverse
top level: BBa_K864050
start: 712
end: 719
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202470
display id: annotation2202470
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202470/range2202470/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202470/range2202470/1
type: Range
persistent identity: range2202470
display id: range2202470
version: 1
direction: direction/reverse
top level: BBa_K864050
start: 582
end: 589
orientation: inline

Repeated sequence
type: SequenceAnnotation
persistent identity: annotation2202476
display id: annotation2202476
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202476/range2202476/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202476/range2202476/1
type: Range
persistent identity: range2202476
display id: range2202476
version: 1
direction: direction/reverse
top level: BBa_K864050
start: 676
end: 683
orientation: inline

Repeat structure
type: SequenceAnnotation
persistent identity: annotation2202466
display id: annotation2202466
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202466/range2202466/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202466/range2202466/1
type: Range
persistent identity: range2202466
display id: range2202466
version: 1
direction: direction/forward
top level: BBa_K864050
start: 190
end: 204
orientation: inline

Repeat structure
type: SequenceAnnotation
persistent identity: annotation2202467
display id: annotation2202467
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202467/range2202467/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202467/range2202467/1
type: Range
persistent identity: range2202467
display id: range2202467
version: 1
direction: direction/forward
top level: BBa_K864050
start: 232
end: 247
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2202480
display id: annotation2202480
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202480/range2202480/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202480/range2202480/1
type: Range
persistent identity: range2202480
display id: range2202480
version: 1
direction: direction/forward
top level: BBa_K864050
start: 702
end: 723
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2202473
display id: annotation2202473
version: 1
top level: BBa_K864050
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864050/annotation2202473/range2202473/1

https://synbiohub.org/public/igem/BBa_K864050/annotation2202473/range2202473/1
type: Range
persistent identity: range2202473
display id: range2202473
version: 1
direction: direction/forward
top level: BBa_K864050
start: 631
end: 652
orientation: inline

Ori+Kan
type: ComponentDefinition
persistent identity: BBa K320004
display id: BBa_K320004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Origin for Asaia and resistance to Kanamycin
creator: Perrudet Christian
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _439_
m user id: 0
owner id: 7149
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K320000
sequence annotation: KanR
sequence annotation: Asaia replication origin
sequence: https://synbiohub.org/public/igem/BBa_K320004_sequence/1

BBa_K320000
type: SequenceAnnotation
persistent identity: annotation2088717
display id: annotation2088717
version: 1
top level: Ori+Kan
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2088717/range2088717/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2088717/range2088717/1
type: Range
persistent identity: range2088717
display id: range2088717
version: 1
direction: direction/reverse
top level: Ori+Kan
start: 1
end: 1399
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2091398
display id: annotation2091398
version: 1
top level: Ori+Kan
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2091398/range2091398/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2091398/range2091398/1
type: Range
persistent identity: range2091398
display id: range2091398
version: 1
direction: direction/forward
top level: Ori+Kan
start: 1400
end: 2372
orientation: inline

Asaia replication origin
type: SequenceAnnotation
persistent identity: annotation2091397
display id: annotation2091397
version: 1
top level: Ori+Kan
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2091397/range2091397/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2091397/range2091397/1
type: Range
persistent identity: range2091397
display id: range2091397
version: 1
direction: direction/forward
top level: Ori+Kan
start: 355
end: 1029
orientation: inline

OriV
type: ComponentDefinition
persistent identity: BBa K125340
display id: BBa_K125340
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Origin of Vegetative Replication
creator: Margaret Ruzicka
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmids containing oriV and the corresponding Rep proteins (from RSF1010) are autonomously replicated in a broad range of hosts. It has been reported that RSF1010 derived plasmids containing oriV and the Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, Serratia (Leemans 1987), and several cyanobacteria strains (Mermet-Bouvier 1993) including Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301.
mutable notes: This part is to be constructed with the Rep proteins derived from an RSF1010 plasmid.
mutable provenance: This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RepB Binding Site
sequence annotation: RepA Binding Site
sequence annotation: RepC Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K125340_sequence/1

RepB Binding Site
type: SequenceAnnotation
persistent identity: annotation1974215
display id: annotation1974215
version: 1
top level: OriV
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974215/range1974215/1

https://synbiohub.org/public/igem/BBa_K125340/annotation1974215/range1974215/1
type: Range
persistent identity: range1974215
display id: range1974215
version: 1
direction: direction/forward
top level: OriV
start: 207
end: 352
orientation: inline

RepA Binding Site
type: SequenceAnnotation
persistent identity: annotation1974216
display id: annotation1974216
version: 1
top level: OriV
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974216/range1974216/1

https://synbiohub.org/public/igem/BBa_K125340/annotation1974216/range1974216/1
type: Range
persistent identity: range1974216
display id: range1974216
version: 1
direction: direction/forward
top level: OriV
start: 353
end: 401
orientation: inline

RepC Binding Site
type: SequenceAnnotation
persistent identity: annotation1974214
display id: annotation1974214
version: 1
top level: OriV
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974214/range1974214/1

https://synbiohub.org/public/igem/BBa_K125340/annotation1974214/range1974214/1
type: Range
persistent identity: range1974214
display id: range1974214
version: 1
direction: direction/forward
top level: OriV
start: 3
end: 66
orientation: inline

pSC101 ts
type: ComponentDefinition
persistent identity: BBa I50052
display id: BBa_I50052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSC101 temperature sensitive origin of replication
creator: Reshma Shetty
created: 2010-06-20T11:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_I50052 is a pSC101 temperature sensitive origin of replication. BBa_I50052 is the temperature sensitive replication origin found in the pSB8* series of BioBrick vectors as well as the pSIM5 recombineering vector.
mutable notes: See design notes for Part:BBa_I50042.
mutable provenance: This part is derived from BBa_I50042. It contains a point mutation that changes Alanine to Valine in RepA protein.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: inverted repeat
sequence annotation: promoter region
sequence annotation: C to T difference with canonical pSC101 vector sequence
sequence annotation: direct repeat
sequence annotation: G to A difference with canonical pSC101 vector sequence
sequence annotation: -10
sequence annotation: par region
sequence annotation: mutated SpeI site
sequence annotation: repA
sequence annotation: T to C difference with canonical pSC101 vector sequence
sequence annotation: Terminator
sequence annotation: -35
sequence annotation: AT rich region
sequence annotation: RBS
sequence annotation: G to C difference with canonical pSC101 vector sequence
sequence annotation: putative polyA
sequence annotation: GCT->GTT ts mutation (Ala->Val)
sequence annotation: Tn3 integration site
sequence: https://synbiohub.org/public/igem/BBa_I50052_sequence/1

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071340
display id: annotation2071340
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071340/range2071340/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071340/range2071340/1
type: Range
persistent identity: range2071340
display id: range2071340
version: 1
direction: direction/forward
top level: pSC101 ts
start: 165
end: 181
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation2071350
display id: annotation2071350
version: 1
top level: pSC101 ts
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071350/range2071350/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071350/range2071350/1
type: Range
persistent identity: range2071350
display id: range2071350
version: 1
direction: direction/forward
top level: pSC101 ts
start: 846
end: 865
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071352
display id: annotation2071352
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071352/range2071352/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071352/range2071352/1
type: Range
persistent identity: range2071352
display id: range2071352
version: 1
direction: direction/forward
top level: pSC101 ts
start: 862
end: 884
orientation: inline

C to T difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071360
display id: annotation2071360
version: 1
top level: pSC101 ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071360/range2071360/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071360/range2071360/1
type: Range
persistent identity: range2071360
display id: range2071360
version: 1
direction: direction/forward
top level: pSC101 ts
start: 1750
end: 1750
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071345
display id: annotation2071345
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071345/range2071345/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071345/range2071345/1
type: Range
persistent identity: range2071345
display id: range2071345
version: 1
direction: direction/forward
top level: pSC101 ts
start: 737
end: 756
orientation: inline

G to A difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071358
display id: annotation2071358
version: 1
top level: pSC101 ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071358/range2071358/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071358/range2071358/1
type: Range
persistent identity: range2071358
display id: range2071358
version: 1
direction: direction/forward
top level: pSC101 ts
start: 1655
end: 1655
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2071351
display id: annotation2071351
version: 1
top level: pSC101 ts
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071351/range2071351/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071351/range2071351/1
type: Range
persistent identity: range2071351
display id: range2071351
version: 1
direction: direction/forward
top level: pSC101 ts
start: 850
end: 855
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation2071337
display id: annotation2071337
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071337/range2071337/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071337/range2071337/1
type: Range
persistent identity: range2071337
display id: range2071337
version: 1
direction: direction/forward
top level: pSC101 ts
start: 137
end: 504
orientation: inline

mutated SpeI site
type: SequenceAnnotation
persistent identity: annotation2071347
display id: annotation2071347
version: 1
top level: pSC101 ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071347/range2071347/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071347/range2071347/1
type: Range
persistent identity: range2071347
display id: range2071347
version: 1
direction: direction/forward
top level: pSC101 ts
start: 814
end: 819
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2071355
display id: annotation2071355
version: 1
top level: pSC101 ts
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071355/range2071355/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071355/range2071355/1
type: Range
persistent identity: range2071355
display id: range2071355
version: 1
direction: direction/forward
top level: pSC101 ts
start: 886
end: 1836
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071341
display id: annotation2071341
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071341/range2071341/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071341/range2071341/1
type: Range
persistent identity: range2071341
display id: range2071341
version: 1
direction: direction/forward
top level: pSC101 ts
start: 192
end: 215
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071338
display id: annotation2071338
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071338/range2071338/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071338/range2071338/1
type: Range
persistent identity: range2071338
display id: range2071338
version: 1
direction: direction/forward
top level: pSC101 ts
start: 139
end: 152
orientation: inline

T to C difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071362
display id: annotation2071362
version: 1
top level: pSC101 ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071362/range2071362/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071362/range2071362/1
type: Range
persistent identity: range2071362
display id: range2071362
version: 1
direction: direction/forward
top level: pSC101 ts
start: 2002
end: 2002
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2071336
display id: annotation2071336
version: 1
top level: pSC101 ts
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071336/range2071336/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071336/range2071336/1
type: Range
persistent identity: range2071336
display id: range2071336
version: 1
direction: direction/forward
top level: pSC101 ts
start: 46
end: 75
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2071348
display id: annotation2071348
version: 1
top level: pSC101 ts
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071348/range2071348/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071348/range2071348/1
type: Range
persistent identity: range2071348
display id: range2071348
version: 1
direction: direction/forward
top level: pSC101 ts
start: 828
end: 833
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071344
display id: annotation2071344
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071344/range2071344/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071344/range2071344/1
type: Range
persistent identity: range2071344
display id: range2071344
version: 1
direction: direction/forward
top level: pSC101 ts
start: 716
end: 735
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071346
display id: annotation2071346
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071346/range2071346/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071346/range2071346/1
type: Range
persistent identity: range2071346
display id: range2071346
version: 1
direction: direction/forward
top level: pSC101 ts
start: 769
end: 788
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation2071343
display id: annotation2071343
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071343/range2071343/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071343/range2071343/1
type: Range
persistent identity: range2071343
display id: range2071343
version: 1
direction: direction/forward
top level: pSC101 ts
start: 635
end: 717
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2071354
display id: annotation2071354
version: 1
top level: pSC101 ts
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071354/range2071354/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071354/range2071354/1
type: Range
persistent identity: range2071354
display id: range2071354
version: 1
direction: direction/forward
top level: pSC101 ts
start: 879
end: 884
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2071353
display id: annotation2071353
version: 1
top level: pSC101 ts
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071353/range2071353/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071353/range2071353/1
type: Range
persistent identity: range2071353
display id: range2071353
version: 1
direction: direction/forward
top level: pSC101 ts
start: 870
end: 882
orientation: inline

G to C difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071361
display id: annotation2071361
version: 1
top level: pSC101 ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071361/range2071361/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071361/range2071361/1
type: Range
persistent identity: range2071361
display id: range2071361
version: 1
direction: direction/forward
top level: pSC101 ts
start: 1912
end: 1912
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071349
display id: annotation2071349
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071349/range2071349/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071349/range2071349/1
type: Range
persistent identity: range2071349
display id: range2071349
version: 1
direction: direction/forward
top level: pSC101 ts
start: 838
end: 859
orientation: inline

putative polyA
type: SequenceAnnotation
persistent identity: annotation2071359
display id: annotation2071359
version: 1
top level: pSC101 ts
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071359/range2071359/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071359/range2071359/1
type: Range
persistent identity: range2071359
display id: range2071359
version: 1
direction: direction/forward
top level: pSC101 ts
start: 1947
end: 1962
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071356
display id: annotation2071356
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071356/range2071356/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071356/range2071356/1
type: Range
persistent identity: range2071356
display id: range2071356
version: 1
direction: direction/forward
top level: pSC101 ts
start: 1847
end: 1861
orientation: inline

GCT->GTT ts mutation (Ala->Val)
type: SequenceAnnotation
persistent identity: annotation2071357
display id: annotation2071357
version: 1
top level: pSC101 ts
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071357/range2071357/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071357/range2071357/1
type: Range
persistent identity: range2071357
display id: range2071357
version: 1
direction: direction/forward
top level: pSC101 ts
start: 1051
end: 1053
orientation: inline

Tn3 integration site
type: SequenceAnnotation
persistent identity: annotation2071339
display id: annotation2071339
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071339/range2071339/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071339/range2071339/1
type: Range
persistent identity: range2071339
display id: range2071339
version: 1
direction: direction/forward
top level: pSC101 ts
start: 158
end: 162
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071342
display id: annotation2071342
version: 1
top level: pSC101 ts
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50052/annotation2071342/range2071342/1

https://synbiohub.org/public/igem/BBa_I50052/annotation2071342/range2071342/1
type: Range
persistent identity: range2071342
display id: range2071342
version: 1
direction: direction/forward
top level: pSC101 ts
start: 221
end: 238
orientation: inline

Ori
type: ComponentDefinition
persistent identity: BBa K320000
display id: BBa_K320000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Asaia replication origin
creator: Perrudet Christian
created: 2010-10-16T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _439_
m user id: 0
owner id: 7149
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: test1
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Asaia replication origin
sequence: https://synbiohub.org/public/igem/BBa_K320000_sequence/1

Asaia replication origin
type: SequenceAnnotation
persistent identity: annotation2091381
display id: annotation2091381
version: 1
top level: Ori
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K320000/annotation2091381/range2091381/1

https://synbiohub.org/public/igem/BBa_K320000/annotation2091381/range2091381/1
type: Range
persistent identity: range2091381
display id: range2091381
version: 1
direction: direction/forward
top level: Ori
start: 371
end: 1046
orientation: inline

BBa_K112125
type: ComponentDefinition
persistent identity: BBa K112125
display id: BBa_K112125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriV origin of replication
creator: Christina Brown
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] <!-- Add more about the biology of this part here
mutable notes: none
mutable provenance: cloned from E. coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K112125_sequence/1

R6K
type: ComponentDefinition
persistent identity: BBa J61001
display id: BBa_J61001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [R6K] Origin of replication
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature. {pSB1A2-Bca1011}
mutable notes: N/A
mutable provenance: PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is Bca1011 ---- ca1011F Forward Biobricking of R6K GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg ca1011R Reverse biobricking of R6K ccgctactagtaGCAGTTCAACCTGTTGATAG
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: R6K
sequence: https://synbiohub.org/public/igem/BBa_J61001_sequence/1

R6K
type: SequenceAnnotation
persistent identity: annotation1892271
display id: annotation1892271
version: 1
top level: R6K
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1

https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1
type: Range
persistent identity: range1892271
display id: range1892271
version: 1
direction: direction/forward
top level: R6K
start: 27
end: 406
orientation: inline

BBa_K533000
type: ComponentDefinition
persistent identity: BBa K533000
display id: BBa_K533000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p15A replication origin
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: p15A replication origin belongs to a different compatibility group from the common pMB1 (pBR or pUC) origin, and hence allows for co-existence with other pUC origin plasmids. When using this part, ligate it along with a proper antibiotic resistance gene and your gene of interest together to make a circular plasmid. Please do NOT include any other replication origin in your design.
mutable notes: Unlike pMB1 origin, which allows for chloramphenicol amplification, p15A replication origin is insensitive to chloramphenicol and remains in low copy number. This replication origin is different from the original one in that it contains several mutations, which increases the copy number to be no lower than pBR origin. In this way, both the compatibility and the convenience for use are accommodated.
mutable provenance: Source: Cloned from plasmid pACYC184
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: p15A replication origin
sequence: https://synbiohub.org/public/igem/BBa_K533000_sequence/1

p15A replication origin
type: SequenceAnnotation
persistent identity: annotation2150403
display id: annotation2150403
version: 1
top level: BBa_K533000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K533000/annotation2150403/range2150403/1

https://synbiohub.org/public/igem/BBa_K533000/annotation2150403/range2150403/1
type: Range
persistent identity: range2150403
display id: range2150403
version: 1
direction: direction/forward
top level: BBa_K533000
start: 1
end: 914
orientation: inline

p15A
type: ComponentDefinition
persistent identity: BBa I50032
display id: BBa_I50032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p15A replication origin
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: p15A origin of replication which is supposed to have low to medium copy number. It is also the origin of replication in pSB3* series plasmids.
mutable notes: The boundaries of this part are quite liberal to ensure that this part is a functional origin.
mutable provenance: This part was constructed by PCR amplification of pSB3K3-P1010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: repeat region
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/igem/BBa_I50032_sequence/1

repeat region
type: SequenceAnnotation
persistent identity: annotation1928308
display id: annotation1928308
version: 1
top level: p15A
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50032/annotation1928308/range1928308/1

https://synbiohub.org/public/igem/BBa_I50032/annotation1928308/range1928308/1
type: Range
persistent identity: range1928308
display id: range1928308
version: 1
direction: direction/forward
top level: p15A
start: 1223
end: 1450
orientation: inline

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation1928307
display id: annotation1928307
version: 1
top level: p15A
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50032/annotation1928307/range1928307/1

https://synbiohub.org/public/igem/BBa_I50032/annotation1928307/range1928307/1
type: Range
persistent identity: range1928307
display id: range1928307
version: 1
direction: direction/forward
top level: p15A
start: 399
end: 1223
orientation: inline

F plasmid
type: ComponentDefinition
persistent identity: BBa I50000
display id: BBa_I50000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F plasmid backbone with BioBrick sites removed.
creator: Reshma Shetty
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a (near) single copy origin derived from the F plasmid. The naturally occurring BioBricks sites were removed with an intent to preserve the amino acid and codon frequency where possible. Similar to the pSMART VC sequence from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. It is intended to be combined with other vector components (antibiotic resistance marker, multiple cloning site) in order to form a BioBricks vector. Relevant information can be found at http://openwetware.org/wiki/BioBrick_Parts_for_Plasmid_Engineering.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: A to T mutation (eliminates SpeI site)
sequence annotation: parA
sequence annotation: iteron (repeat region)
sequence annotation: repE
sequence annotation: C to G mutation (eliminates XbaI site, noncoding region)
sequence annotation: DnaA box
sequence annotation: C to T mutation (eliminates PstI site)
sequence annotation: A/T region
sequence annotation: sequence derived from F plasmid in GenBank Accession Number AP001918
sequence annotation: ori2 (oriS) single copy replication origin
sequence annotation: sopC(IncD) repeat region
sequence annotation: parB
sequence: https://synbiohub.org/public/igem/BBa_I50000_sequence/1

A to T mutation (eliminates SpeI site)
type: SequenceAnnotation
persistent identity: annotation1757411
display id: annotation1757411
version: 1
top level: F plasmid
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757411/range1757411/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757411/range1757411/1
type: Range
persistent identity: range1757411
display id: range1757411
version: 1
direction: direction/reverse
top level: F plasmid
start: 3711
end: 3711
orientation: inline

parA
type: SequenceAnnotation
persistent identity: annotation1757406
display id: annotation1757406
version: 1
top level: F plasmid
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757406/range1757406/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757406/range1757406/1
type: Range
persistent identity: range1757406
display id: range1757406
version: 1
direction: direction/forward
top level: F plasmid
start: 1718
end: 2893
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757417
display id: annotation1757417
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757417/range1757417/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757417/range1757417/1
type: Range
persistent identity: range1757417
display id: range1757417
version: 1
direction: direction/forward
top level: F plasmid
start: 213
end: 232
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1757408
display id: annotation1757408
version: 1
top level: F plasmid
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757408/range1757408/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757408/range1757408/1
type: Range
persistent identity: range1757408
display id: range1757408
version: 1
direction: direction/forward
top level: F plasmid
start: 384
end: 1139
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757416
display id: annotation1757416
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757416/range1757416/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757416/range1757416/1
type: Range
persistent identity: range1757416
display id: range1757416
version: 1
direction: direction/forward
top level: F plasmid
start: 192
end: 211
orientation: inline

C to G mutation (eliminates XbaI site, noncoding region)
type: SequenceAnnotation
persistent identity: annotation1757413
display id: annotation1757413
version: 1
top level: F plasmid
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757413/range1757413/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757413/range1757413/1
type: Range
persistent identity: range1757413
display id: range1757413
version: 1
direction: direction/reverse
top level: F plasmid
start: 183
end: 183
orientation: inline

DnaA box
type: SequenceAnnotation
persistent identity: annotation1757420
display id: annotation1757420
version: 1
top level: F plasmid
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757420/range1757420/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757420/range1757420/1
type: Range
persistent identity: range1757420
display id: range1757420
version: 1
direction: direction/forward
top level: F plasmid
start: 81
end: 88
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757418
display id: annotation1757418
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757418/range1757418/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757418/range1757418/1
type: Range
persistent identity: range1757418
display id: range1757418
version: 1
direction: direction/forward
top level: F plasmid
start: 246
end: 265
orientation: inline

C to T mutation (eliminates PstI site)
type: SequenceAnnotation
persistent identity: annotation1757412
display id: annotation1757412
version: 1
top level: F plasmid
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757412/range1757412/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757412/range1757412/1
type: Range
persistent identity: range1757412
display id: range1757412
version: 1
direction: direction/reverse
top level: F plasmid
start: 1013
end: 1013
orientation: inline

DnaA box
type: SequenceAnnotation
persistent identity: annotation1757414
display id: annotation1757414
version: 1
top level: F plasmid
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757414/range1757414/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757414/range1757414/1
type: Range
persistent identity: range1757414
display id: range1757414
version: 1
direction: direction/forward
top level: F plasmid
start: 89
end: 97
orientation: inline

A/T region
type: SequenceAnnotation
persistent identity: annotation1757415
display id: annotation1757415
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757415/range1757415/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757415/range1757415/1
type: Range
persistent identity: range1757415
display id: range1757415
version: 1
direction: direction/forward
top level: F plasmid
start: 101
end: 147
orientation: inline

C to T mutation (eliminates PstI site)
type: SequenceAnnotation
persistent identity: annotation1757410
display id: annotation1757410
version: 1
top level: F plasmid
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757410/range1757410/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757410/range1757410/1
type: Range
persistent identity: range1757410
display id: range1757410
version: 1
direction: direction/reverse
top level: F plasmid
start: 2553
end: 2553
orientation: inline

sequence derived from F plasmid in GenBank Accession Number AP001918
type: SequenceAnnotation
persistent identity: annotation1757407
display id: annotation1757407
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757407/range1757407/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757407/range1757407/1
type: Range
persistent identity: range1757407
display id: range1757407
version: 1
direction: direction/reverse
top level: F plasmid
start: 1
end: 1390
orientation: inline

iteron (repeat region)
type: SequenceAnnotation
persistent identity: annotation1757419
display id: annotation1757419
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757419/range1757419/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757419/range1757419/1
type: Range
persistent identity: range1757419
display id: range1757419
version: 1
direction: direction/forward
top level: F plasmid
start: 268
end: 287
orientation: inline

sequence derived from F plasmid in GenBank Accession Number AP001918
type: SequenceAnnotation
persistent identity: annotation1757421
display id: annotation1757421
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757421/range1757421/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757421/range1757421/1
type: Range
persistent identity: range1757421
display id: range1757421
version: 1
direction: direction/reverse
top level: F plasmid
start: 1720
end: 4640
orientation: inline

ori2 (oriS) single copy replication origin
type: SequenceAnnotation
persistent identity: annotation1757409
display id: annotation1757409
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757409/range1757409/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757409/range1757409/1
type: Range
persistent identity: range1757409
display id: range1757409
version: 1
direction: direction/reverse
top level: F plasmid
start: 1
end: 290
orientation: inline

sopC(IncD) repeat region
type: SequenceAnnotation
persistent identity: annotation1757395
display id: annotation1757395
version: 1
top level: F plasmid
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757395/range1757395/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757395/range1757395/1
type: Range
persistent identity: range1757395
display id: range1757395
version: 1
direction: direction/reverse
top level: F plasmid
start: 3938
end: 4640
orientation: inline

parB
type: SequenceAnnotation
persistent identity: annotation1757405
display id: annotation1757405
version: 1
top level: F plasmid
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I50000/annotation1757405/range1757405/1

https://synbiohub.org/public/igem/BBa_I50000/annotation1757405/range1757405/1
type: Range
persistent identity: range1757405
display id: range1757405
version: 1
direction: direction/forward
top level: F plasmid
start: 2893
end: 3864
orientation: inline