//plasmid/expression/t7
type: Collection
persistent identity: plasmid expression t7
display id: plasmid_expression_t7
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1428000
member: BBa_K533003
member: BBa_K921001
member: T7start
member: BBa_K1680026
member: T7RSR
member: BBa_K559010
member: BBa_K533002
member: BBa_K921002
member: BBa_K921000
member: BBa_K1614000
member: BBa_K533001
member: T7p
member: T7stop
member: BBa_K559001

//plasmid/expression
type: Collection
persistent identity: plasmid expression
display id: plasmid_expression
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: xyl+efe
member: BBa_K2127001
member: BBa_K786003
member: //plasmid/expression/t7
member: pSBBs4S
member: pEX
member: BBa_K2074133
member: BBa_K1680026
member: BBa_K786001
member: BBa_K786004
member: BBa_I714946
member: BBa_K2127002
member: BBa_K786002
member: pJEJAM1
member: BBa_K819008

BBa_K559001
type: ComponentDefinition
persistent identity: BBa K559001
display id: BBa_K559001
version: 1
was generated by: iGEM to SBOL conversion
description: Halorhodopsin under T7 promoter
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: Halorhodopsin come from N.pharaonis, gene bank J05199.1
owned by: james
owned by: myers
mutable description: Halorhodopsin under constitute T7 promoter to regulate the gene transcription to be maximized and no restrict to other regulatory factor
discontinued: false
dominant: false
experience: experience/None
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The coding sequence of N.pharaonis J05199.1 is selected from 61nt to 700nt and the T7 promotor placed in front by 3A assembly
star: false
creator: Jacky, Fong Chuen, Loo
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: T7 promotor
sequence annotation: Halorhodopsin
sequence annotation: lac repressor
sequence: https://synbiohub.org/public/igem/BBa_K559001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2177555
display id: annotation2177555
version: 1
top level: BBa_K559001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177555/range2177555/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177555/range2177555/1
type: Range
persistent identity: range2177555
display id: range2177555
version: 1
top level: BBa_K559001
direction: direction/forward
start: 66
end: 77
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2177551
display id: annotation2177551
version: 1
top level: BBa_K559001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177551/range2177551/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177551/range2177551/1
type: Range
persistent identity: range2177551
display id: range2177551
version: 1
top level: BBa_K559001
direction: direction/forward
start: 1
end: 16
orientation: inline

Halorhodopsin
type: SequenceAnnotation
persistent identity: annotation2177554
display id: annotation2177554
version: 1
top level: BBa_K559001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177554/range2177554/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177554/range2177554/1
type: Range
persistent identity: range2177554
display id: range2177554
version: 1
top level: BBa_K559001
direction: direction/forward
start: 89
end: 728
orientation: inline

lac repressor
type: SequenceAnnotation
persistent identity: annotation2177553
display id: annotation2177553
version: 1
top level: BBa_K559001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177553/range2177553/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177553/range2177553/1
type: Range
persistent identity: range2177553
display id: range2177553
version: 1
top level: BBa_K559001
direction: direction/forward
start: 22
end: 42
orientation: inline

BBa_K921002
type: ComponentDefinition
persistent identity: BBa K921002
display id: BBa_K921002
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP + IPTG->PoPs (Mutant III)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
star: false
creator: Eric Pederson
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: T7 Promoter
sequence annotation: LacO
sequence annotation: Initiation
sequence: https://synbiohub.org/public/igem/BBa_K921002_sequence/1

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205316
display id: annotation2205316
version: 1
top level: BBa_K921002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1
type: Range
persistent identity: range2205316
display id: range2205316
version: 1
top level: BBa_K921002
direction: direction/forward
start: 1
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205317
display id: annotation2205317
version: 1
top level: BBa_K921002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1
type: Range
persistent identity: range2205317
display id: range2205317
version: 1
top level: BBa_K921002
direction: direction/forward
start: 19
end: 47
orientation: inline

Initiation
type: SequenceAnnotation
persistent identity: annotation2205318
display id: annotation2205318
version: 1
top level: BBa_K921002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1
type: Range
persistent identity: range2205318
display id: range2205318
version: 1
top level: BBa_K921002
direction: direction/forward
start: 18
end: 20
orientation: inline

BBa_K533003
type: ComponentDefinition
persistent identity: BBa K533003
display id: BBa_K533003
version: 1
was generated by: iGEM to SBOL conversion
description: OmpA-HIV aspartyl protease
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
mutable provenance: HIV protease sequence is from former part BBa_I712667. OmpA sequence is from BL21(DE3) e. Coli strain.
owned by: james
owned by: myers
mutable description: OmpA is used to present HIV aspartyl protease outside the E. Coli membrane so that it can cut other proteins with the specific sequence outside in the media.
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Since HIV protease is a dimer, dimerization is a great concern to its function. Function of this protease is tested in BL21.
star: false
creator: ZHANG Yunxiao
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: rbs
sequence annotation: T7
sequence annotation: START
sequence annotation: BBa_I712667
sequence annotation: lacI
sequence annotation: OmpA
sequence annotation: STOP
sequence annotation: HIV protease
sequence: https://synbiohub.org/public/igem/BBa_K533003_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2150442
display id: annotation2150442
version: 1
top level: BBa_K533003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150442/range2150442/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2150442/range2150442/1
type: Range
persistent identity: range2150442
display id: range2150442
version: 1
top level: BBa_K533003
direction: direction/forward
start: 75
end: 79
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2150439
display id: annotation2150439
version: 1
top level: BBa_K533003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150439/range2150439/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2150439/range2150439/1
type: Range
persistent identity: range2150439
display id: range2150439
version: 1
top level: BBa_K533003
direction: direction/forward
start: 1
end: 18
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2146916
display id: annotation2146916
version: 1
top level: BBa_K533003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146916/range2146916/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146916/range2146916/1
type: Range
persistent identity: range2146916
display id: range2146916
version: 1
top level: BBa_K533003
direction: direction/forward
start: 87
end: 89
orientation: inline

BBa_I712667
type: SequenceAnnotation
persistent identity: annotation2146918
display id: annotation2146918
version: 1
top level: BBa_K533003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146918/range2146918/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146918/range2146918/1
type: Range
persistent identity: range2146918
display id: range2146918
version: 1
top level: BBa_K533003
direction: direction/reverse
start: 633
end: 935
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2150440
display id: annotation2150440
version: 1
top level: BBa_K533003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150440/range2150440/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2150440/range2150440/1
type: Range
persistent identity: range2150440
display id: range2150440
version: 1
top level: BBa_K533003
direction: direction/forward
start: 20
end: 44
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2150441
display id: annotation2150441
version: 1
top level: BBa_K533003
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150441/range2150441/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2150441/range2150441/1
type: Range
persistent identity: range2150441
display id: range2150441
version: 1
top level: BBa_K533003
direction: direction/forward
start: 87
end: 632
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2146917
display id: annotation2146917
version: 1
top level: BBa_K533003
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146917/range2146917/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146917/range2146917/1
type: Range
persistent identity: range2146917
display id: range2146917
version: 1
top level: BBa_K533003
direction: direction/forward
start: 933
end: 935
orientation: inline

HIV protease
type: SequenceAnnotation
persistent identity: annotation2146915
display id: annotation2146915
version: 1
top level: BBa_K533003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146915/range2146915/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146915/range2146915/1
type: Range
persistent identity: range2146915
display id: range2146915
version: 1
top level: BBa_K533003
direction: direction/forward
start: 633
end: 935
orientation: inline

BBa_I714946
type: ComponentDefinition
persistent identity: BBa I714946
display id: BBa_I714946
version: 1
was generated by: iGEM to SBOL conversion
description: pCC002
creator: Xili Liu
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2250
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: with a Ptet promoter controled by TetR, a MCS, T1 terminator, p15A ori and Cm resistence gene. copy number: 15~20
mutable notes: to measure the efficiency of different SD signal, the plasmid has a MCS downstream of Ptet where can insert reporters along with SD signal. There is a T1 terminator downstream of MCS to terminate transcription starting from Ptet. Ptet is repressed by TetR carried on this plasmid and can be turned on by adding aTc.
mutable provenance: pCRT-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: C0040
sequence annotation: B0010
sequence annotation: p15A ori
sequence annotation: MCS
sequence annotation: promoter of cat
sequence annotation: R0040
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_I714946_sequence/1

C0040
type: SequenceAnnotation
persistent identity: annotation1955250
display id: annotation1955250
version: 1
top level: BBa_I714946
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955250/range1955250/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955250/range1955250/1
type: Range
persistent identity: range1955250
display id: range1955250
version: 1
direction: direction/forward
top level: BBa_I714946
start: 1279
end: 1909
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation1955248
display id: annotation1955248
version: 1
top level: BBa_I714946
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955248/range1955248/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955248/range1955248/1
type: Range
persistent identity: range1955248
display id: range1955248
version: 1
direction: direction/forward
top level: BBa_I714946
start: 298
end: 388
orientation: inline

p15A ori
type: SequenceAnnotation
persistent identity: annotation1955249
display id: annotation1955249
version: 1
top level: BBa_I714946
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955249/range1955249/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955249/range1955249/1
type: Range
persistent identity: range1955249
display id: range1955249
version: 1
direction: direction/forward
top level: BBa_I714946
start: 431
end: 1179
orientation: inline

MCS
type: SequenceAnnotation
persistent identity: annotation1955247
display id: annotation1955247
version: 1
top level: BBa_I714946
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955247/range1955247/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955247/range1955247/1
type: Range
persistent identity: range1955247
display id: range1955247
version: 1
direction: direction/forward
top level: BBa_I714946
start: 224
end: 286
orientation: inline

promoter of cat
type: SequenceAnnotation
persistent identity: annotation1955245
display id: annotation1955245
version: 1
top level: BBa_I714946
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955245/range1955245/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955245/range1955245/1
type: Range
persistent identity: range1955245
display id: range1955245
version: 1
direction: direction/reverse
top level: BBa_I714946
start: 74
end: 103
orientation: inline

R0040
type: SequenceAnnotation
persistent identity: annotation1955246
display id: annotation1955246
version: 1
top level: BBa_I714946
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955246/range1955246/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955246/range1955246/1
type: Range
persistent identity: range1955246
display id: range1955246
version: 1
direction: direction/forward
top level: BBa_I714946
start: 130
end: 177
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation1955262
display id: annotation1955262
version: 1
top level: BBa_I714946
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955262/range1955262/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955262/range1955262/1
type: Range
persistent identity: range1955262
display id: range1955262
version: 1
direction: direction/forward
top level: BBa_I714946
start: 2277
end: 2936
orientation: inline

BBa_K786004
type: ComponentDefinition
persistent identity: BBa K786004
display id: BBa_K786004
version: 1
was generated by: iGEM to SBOL conversion
description: Red light triggering phototaxis using chp8
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Red light triggering phototaxis using chp8, which can sense the red light from 600-700 nm.
mutable notes: 6 poly-His tag was positioned at C-terminal of the protein.
mutable provenance: The sequence of chp8 is from previous biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: chp8 protein
sequence annotation: RBS 1.0
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: poly-HIS tag
sequence: https://synbiohub.org/public/igem/BBa_K786004_sequence/1

chp8 protein
type: SequenceAnnotation
persistent identity: annotation2190189
display id: annotation2190189
version: 1
top level: BBa_K786004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190189/range2190189/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190189/range2190189/1
type: Range
persistent identity: range2190189
display id: range2190189
version: 1
direction: direction/forward
top level: BBa_K786004
start: 69
end: 2321
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2190188
display id: annotation2190188
version: 1
top level: BBa_K786004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190188/range2190188/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190188/range2190188/1
type: Range
persistent identity: range2190188
display id: range2190188
version: 1
direction: direction/forward
top level: BBa_K786004
start: 51
end: 62
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190187
display id: annotation2190187
version: 1
top level: BBa_K786004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190187/range2190187/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190187/range2190187/1
type: Range
persistent identity: range2190187
display id: range2190187
version: 1
direction: direction/forward
top level: BBa_K786004
start: 1
end: 50
orientation: inline

poly-HIS tag
type: SequenceAnnotation
persistent identity: annotation2190190
display id: annotation2190190
version: 1
top level: BBa_K786004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190190/range2190190/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190190/range2190190/1
type: Range
persistent identity: range2190190
display id: range2190190
version: 1
direction: direction/forward
top level: BBa_K786004
start: 2301
end: 2321
orientation: inline

pSBBs4S
type: ComponentDefinition
persistent identity: BBa K823024
display id: BBa_K823024
version: 1
was generated by: iGEM to SBOL conversion
description: pSBBs4S-Pxyl: Integrative expression vector for Bacillus subtilis</i
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
mutable provenance: This part is an igem compatible version of the Bacillus expression vector pXT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 'thrC
sequence annotation: thrB
sequence annotation: bla
sequence annotation: thrC'
sequence annotation: spc
sequence annotation: hom
sequence annotation: Pxly region
sequence: https://synbiohub.org/public/igem/BBa_K823024_sequence/1

'thrC
type: SequenceAnnotation
persistent identity: annotation2180241
display id: annotation2180241
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180241/range2180241/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180241/range2180241/1
type: Range
persistent identity: range2180241
display id: range2180241
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1028
end: 1456
orientation: inline

thrB
type: SequenceAnnotation
persistent identity: annotation2180242
display id: annotation2180242
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180242/range2180242/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180242/range2180242/1
type: Range
persistent identity: range2180242
display id: range2180242
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1453
end: 1863
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180243
display id: annotation2180243
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180243/range2180243/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180243/range2180243/1
type: Range
persistent identity: range2180243
display id: range2180243
version: 1
direction: direction/reverse
top level: pSBBs4S
start: 2716
end: 3576
orientation: inline

thrC'
type: SequenceAnnotation
persistent identity: annotation2180245
display id: annotation2180245
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180245/range2180245/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180245/range2180245/1
type: Range
persistent identity: range2180245
display id: range2180245
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4033
end: 4572
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2180240
display id: annotation2180240
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180240/range2180240/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180240/range2180240/1
type: Range
persistent identity: range2180240
display id: range2180240
version: 1
direction: direction/forward
top level: pSBBs4S
start: 211
end: 885
orientation: inline

hom
type: SequenceAnnotation
persistent identity: annotation2180244
display id: annotation2180244
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180244/range2180244/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180244/range2180244/1
type: Range
persistent identity: range2180244
display id: range2180244
version: 1
direction: direction/forward
top level: pSBBs4S
start: 3731
end: 4033
orientation: inline

Pxly region
type: SequenceAnnotation
persistent identity: annotation2180246
display id: annotation2180246
version: 1
top level: pSBBs4S
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180246/range2180246/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180246/range2180246/1
type: Range
persistent identity: range2180246
display id: range2180246
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4539
end: 4763
orientation: inline

BBa_K1428000
type: ComponentDefinition
persistent identity: BBa K1428000
display id: BBa_K1428000
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter-driven BMC structural genes with a His6-tagged component for purification
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: BBa_K311004, pET28a
owned by: james
owned by: myers
mutable description: T7 promoter diving the expression of His6-tagged EutS, with subsequent Plac-driven EutM, EutN, EutL, EutK
discontinued: false
dominant: false
group u list: _1806_
m user id: 0
owner id: 20650
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Clone BBa_K311004 into pET28a via NheI and NdeI
star: false
creator: Lai Hei Ming
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: T7 RBS
sequence annotation: fusion linker (TGS)
sequence annotation: signal peptide
sequence: https://synbiohub.org/public/igem/BBa_K1428000_sequence/1

T7 RBS
type: SequenceAnnotation
persistent identity: annotation2417040
display id: annotation2417040
version: 1
top level: BBa_K1428000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1428000/annotation2417040/range2417040/1

https://synbiohub.org/public/igem/BBa_K1428000/annotation2417040/range2417040/1
type: Range
persistent identity: range2417040
display id: range2417040
version: 1
top level: BBa_K1428000
direction: direction/forward
start: 13
end: 29
orientation: inline

fusion linker (TGS)
type: SequenceAnnotation
persistent identity: annotation2417042
display id: annotation2417042
version: 1
top level: BBa_K1428000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1428000/annotation2417042/range2417042/1

https://synbiohub.org/public/igem/BBa_K1428000/annotation2417042/range2417042/1
type: Range
persistent identity: range2417042
display id: range2417042
version: 1
top level: BBa_K1428000
direction: direction/forward
start: 94
end: 103
orientation: inline

signal peptide
type: SequenceAnnotation
persistent identity: annotation2417041
display id: annotation2417041
version: 1
top level: BBa_K1428000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1428000/annotation2417041/range2417041/1

https://synbiohub.org/public/igem/BBa_K1428000/annotation2417041/range2417041/1
type: Range
persistent identity: range2417041
display id: range2417041
version: 1
top level: BBa_K1428000
direction: direction/forward
start: 37
end: 93
orientation: inline

BBa_K533002
type: ComponentDefinition
persistent identity: BBa K533002
display id: BBa_K533002
version: 1
was generated by: iGEM to SBOL conversion
description: OmpA-HIV protease site-SH3
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
mutable provenance: OmpA is from BL21(DE3) E. Coli genomic DNA. SH3 is from human Grb2 protein. HIV protease site is from a former part.
owned by: james
owned by: myers
mutable description: Added HIV protease site between OmpA and SH3 for easier release.
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The HIV protease site is embedded in the protein so that a proteolytic cleavage can release the binding cassette.
star: false
creator: ZHANG Yunxiao
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: OmpA
sequence annotation: SH3
sequence annotation: T7 promoter
sequence annotation: BBa_I712015
sequence annotation: lacI
sequence: https://synbiohub.org/public/igem/BBa_K533002_sequence/1

OmpA
type: SequenceAnnotation
persistent identity: annotation2150424
display id: annotation2150424
version: 1
top level: BBa_K533002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2150424/range2150424/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2150424/range2150424/1
type: Range
persistent identity: range2150424
display id: range2150424
version: 1
top level: BBa_K533002
direction: direction/forward
start: 87
end: 633
orientation: inline

SH3
type: SequenceAnnotation
persistent identity: annotation2150425
display id: annotation2150425
version: 1
top level: BBa_K533002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2150425/range2150425/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2150425/range2150425/1
type: Range
persistent identity: range2150425
display id: range2150425
version: 1
top level: BBa_K533002
direction: direction/forward
start: 676
end: 857
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2146913
display id: annotation2146913
version: 1
top level: BBa_K533002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2146913/range2146913/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2146913/range2146913/1
type: Range
persistent identity: range2146913
display id: range2146913
version: 1
top level: BBa_K533002
direction: direction/forward
start: 1
end: 18
orientation: inline

BBa_I712015
type: SequenceAnnotation
persistent identity: annotation2146914
display id: annotation2146914
version: 1
top level: BBa_K533002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2146914/range2146914/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2146914/range2146914/1
type: Range
persistent identity: range2146914
display id: range2146914
version: 1
top level: BBa_K533002
direction: direction/forward
start: 634
end: 675
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2150423
display id: annotation2150423
version: 1
top level: BBa_K533002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2150423/range2150423/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2150423/range2150423/1
type: Range
persistent identity: range2150423
display id: range2150423
version: 1
top level: BBa_K533002
direction: direction/forward
start: 20
end: 44
orientation: inline

BBa_K786001
type: ComponentDefinition
persistent identity: BBa K786001
display id: BBa_K786001
version: 1
was generated by: iGEM to SBOL conversion
description: Sensory rhodopsin II (SRII) with HtrII & Tsr, sensitive to blue-green light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sensory rhodopsin II fusion with HtrII & Tsr, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0015
sequence annotation: RBS 1.0
sequence annotation: linker
sequence annotation: HtrII
sequence annotation: BamHI for C-terminal fusion
sequence annotation: Sensory Rhodopsin II
sequence annotation: Tsr
sequence annotation: HindIII for N-terminal fusin
sequence annotation: Constitutive promoter BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K786001_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2199672
display id: annotation2199672
version: 1
top level: BBa_K786001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199672/range2199672/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199672/range2199672/1
type: Range
persistent identity: range2199672
display id: range2199672
version: 1
direction: direction/forward
top level: BBa_K786001
start: 2105
end: 2233
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2199665
display id: annotation2199665
version: 1
top level: BBa_K786001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199665/range2199665/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199665/range2199665/1
type: Range
persistent identity: range2199665
display id: range2199665
version: 1
direction: direction/forward
top level: BBa_K786001
start: 43
end: 55
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2199668
display id: annotation2199668
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199668/range2199668/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199668/range2199668/1
type: Range
persistent identity: range2199668
display id: range2199668
version: 1
direction: direction/forward
top level: BBa_K786001
start: 785
end: 811
orientation: inline

HtrII
type: SequenceAnnotation
persistent identity: annotation2199670
display id: annotation2199670
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199670/range2199670/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199670/range2199670/1
type: Range
persistent identity: range2199670
display id: range2199670
version: 1
direction: direction/forward
top level: BBa_K786001
start: 812
end: 1210
orientation: inline

BamHI for C-terminal fusion
type: SequenceAnnotation
persistent identity: annotation2199669
display id: annotation2199669
version: 1
top level: BBa_K786001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199669/range2199669/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199669/range2199669/1
type: Range
persistent identity: range2199669
display id: range2199669
version: 1
direction: direction/forward
top level: BBa_K786001
start: 785
end: 790
orientation: inline

Sensory Rhodopsin II
type: SequenceAnnotation
persistent identity: annotation2199667
display id: annotation2199667
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199667/range2199667/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199667/range2199667/1
type: Range
persistent identity: range2199667
display id: range2199667
version: 1
direction: direction/forward
top level: BBa_K786001
start: 68
end: 784
orientation: inline

Tsr
type: SequenceAnnotation
persistent identity: annotation2199671
display id: annotation2199671
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199671/range2199671/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199671/range2199671/1
type: Range
persistent identity: range2199671
display id: range2199671
version: 1
direction: direction/forward
top level: BBa_K786001
start: 1210
end: 2070
orientation: inline

HindIII for N-terminal fusin
type: SequenceAnnotation
persistent identity: annotation2199666
display id: annotation2199666
version: 1
top level: BBa_K786001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199666/range2199666/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199666/range2199666/1
type: Range
persistent identity: range2199666
display id: range2199666
version: 1
direction: direction/forward
top level: BBa_K786001
start: 62
end: 67
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199664
display id: annotation2199664
version: 1
top level: BBa_K786001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199664/range2199664/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199664/range2199664/1
type: Range
persistent identity: range2199664
display id: range2199664
version: 1
direction: direction/forward
top level: BBa_K786001
start: 1
end: 42
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0011
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
access: public
definition: BBa_B0012

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
access: public
definition: BBa_B0011

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: stop
sequence annotation: polya
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

xyl+efe
type: ComponentDefinition
persistent identity: BBa K1065204
display id: BBa_K1065204
version: 1
was generated by: iGEM to SBOL conversion
description: Efe+Bba_B0015 in BBa_K823024 (pXyl)
creator: Viola Valentini
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1373_
m user id: 0
owner id: 15998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pXyl vector that has been sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.
mutable notes: ===Design Notes=== The coding sequence was taken from Pseudomonas syringae pv. phaseolicola PK2. We firstly optimized the codons for both B. subtilis and E. coli and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly. GAATTCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGCACC... ...TCGACCGGTTAATACTAGTAGCGGCCGCTGCAG
mutable provenance: This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K823024
component: BBa_K1065002
sequence annotation: BBa_K1065002
sequence annotation: BBa_K823024
sequence: https://synbiohub.org/public/igem/BBa_K1065204_sequence/1

BBa_K823024
type: Component
persistent identity: component2334267
display id: component2334267
version: 1
top level: xyl+efe
access: public
definition: pSBBs4S

BBa_K1065002
type: Component
persistent identity: component2334259
display id: component2334259
version: 1
top level: xyl+efe
access: public
definition: https://synbiohub.org/public/igem/BBa_K1065002/1

BBa_K1065002
type: SequenceAnnotation
persistent identity: annotation2334259
display id: annotation2334259
version: 1
top level: xyl+efe
location: https://synbiohub.org/public/igem/BBa_K1065204/annotation2334259/range2334259/1
component: BBa_K1065002

https://synbiohub.org/public/igem/BBa_K1065204/annotation2334259/range2334259/1
type: Range
persistent identity: range2334259
display id: range2334259
version: 1
direction: direction/reverse
top level: xyl+efe
start: 1
end: 1215
orientation: inline

BBa_K823024
type: SequenceAnnotation
persistent identity: annotation2334267
display id: annotation2334267
version: 1
top level: xyl+efe
location: https://synbiohub.org/public/igem/BBa_K1065204/annotation2334267/range2334267/1
component: BBa_K823024

https://synbiohub.org/public/igem/BBa_K1065204/annotation2334267/range2334267/1
type: Range
persistent identity: range2334267
display id: range2334267
version: 1
direction: direction/reverse
top level: xyl+efe
start: 1224
end: 6017
orientation: inline

BBa_K2127002
type: ComponentDefinition
persistent identity: BBa K2127002
display id: BBa_K2127002
version: 1
was generated by: iGEM to SBOL conversion
description: CP47 His-tagged with Lac Promoter
creator: Dawson Zeng
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:46:10Z
discontinued: false
dominant: false
group u list: _2596_
m user id: 32415
owner id: 17480
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CP47 His-tagged with Lac Promoter synthesized by IDT DNA Inc.
mutable notes: Double Stop Codon. ORF Check
mutable provenance: NCBI Reference Sequence: NC_000911.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_R0010
component: BBa_K2127004
component: BBa_B0030
component: BBa_K2127007
sequence annotation: BBa_B0030
sequence annotation: BBa_R0010
sequence annotation: BBa_K2127004
sequence annotation: BBa_K2127007
sequence: https://synbiohub.org/public/igem/BBa_K2127002_sequence/1

BBa_R0010
type: Component
persistent identity: component2527099
display id: component2527099
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K2127004
type: Component
persistent identity: component2527113
display id: component2527113
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127004/1

BBa_B0030
type: Component
persistent identity: component2527107
display id: component2527107
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K2127007
type: Component
persistent identity: component2527114
display id: component2527114
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127007/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2527107
display id: annotation2527107
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527107/range2527107/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527107/range2527107/1
type: Range
persistent identity: range2527107
display id: range2527107
version: 1
direction: direction/forward
top level: BBa_K2127002
start: 201
end: 215
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2527099
display id: annotation2527099
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527099/range2527099/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527099/range2527099/1
type: Range
persistent identity: range2527099
display id: range2527099
version: 1
direction: direction/forward
top level: BBa_K2127002
start: 1
end: 200
orientation: inline

BBa_K2127004
type: SequenceAnnotation
persistent identity: annotation2527113
display id: annotation2527113
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527113/range2527113/1
component: BBa_K2127004

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527113/range2527113/1
type: Range
persistent identity: range2527113
display id: range2527113
version: 1
direction: direction/reverse
top level: BBa_K2127002
start: 216
end: 1763
orientation: inline

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2527114
display id: annotation2527114
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527114/range2527114/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527114/range2527114/1
type: Range
persistent identity: range2527114
display id: range2527114
version: 1
direction: direction/reverse
top level: BBa_K2127002
start: 1764
end: 1886
orientation: inline

BBa_K1614000
type: ComponentDefinition
persistent identity: BBa K1614000
display id: BBa_K1614000
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter for expression of functional RNA
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T12:29:29Z
mutable provenance: T7 phage minimal promoter (consensus)
owned by: james
owned by: myers
mutable description: Test
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 12380
owner id: 16422
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Stefan Holderbach
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1614000_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2454728
display id: annotation2454728
version: 1
top level: BBa_K1614000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1

https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1
type: Range
persistent identity: range2454728
display id: range2454728
version: 1
top level: BBa_K1614000
direction: direction/forward
start: 1
end: 17
orientation: inline

pJEJAM1
type: ComponentDefinition
persistent identity: BBa K678049
display id: BBa_K678049
version: 1
was generated by: iGEM to SBOL conversion
description: pJEJAM1
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to be announced
mutable notes: to be announced
mutable provenance: to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K678006
component: BBa_K678019
component: BBa_J52034
component: BBa_K678023
component: BBa_K678021
sequence annotation: BBa_K678021
sequence annotation: BBa_K678019
sequence annotation: BBa_J52034
sequence annotation: BBa_K678006
sequence annotation: BBa_K678023
sequence: https://synbiohub.org/public/igem/BBa_K678049_sequence/1

BBa_K678006
type: Component
persistent identity: component2135852
display id: component2135852
version: 1
top level: pJEJAM1
access: public
definition: https://synbiohub.org/public/igem/BBa_K678006/1

BBa_K678019
type: Component
persistent identity: component2135855
display id: component2135855
version: 1
top level: pJEJAM1
access: public
definition: https://synbiohub.org/public/igem/BBa_K678019/1

BBa_J52034
type: Component
persistent identity: component2135848
display id: component2135848
version: 1
top level: pJEJAM1
access: public
definition: https://synbiohub.org/public/igem/BBa_J52034/1

BBa_K678023
type: Component
persistent identity: component2135861
display id: component2135861
version: 1
top level: pJEJAM1
access: public
definition: https://synbiohub.org/public/igem/BBa_K678023/1

BBa_K678021
type: Component
persistent identity: component2135858
display id: component2135858
version: 1
top level: pJEJAM1
access: public
definition: https://synbiohub.org/public/igem/BBa_K678021/1

BBa_K678021
type: SequenceAnnotation
persistent identity: annotation2135858
display id: annotation2135858
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135858/range2135858/1
component: BBa_K678021

https://synbiohub.org/public/igem/BBa_K678049/annotation2135858/range2135858/1
type: Range
persistent identity: range2135858
display id: range2135858
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 1623
end: 3381
orientation: inline

BBa_K678019
type: SequenceAnnotation
persistent identity: annotation2135855
display id: annotation2135855
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135855/range2135855/1
component: BBa_K678019

https://synbiohub.org/public/igem/BBa_K678049/annotation2135855/range2135855/1
type: Range
persistent identity: range2135855
display id: range2135855
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 1390
end: 1622
orientation: inline

BBa_J52034
type: SequenceAnnotation
persistent identity: annotation2135848
display id: annotation2135848
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135848/range2135848/1
component: BBa_J52034

https://synbiohub.org/public/igem/BBa_K678049/annotation2135848/range2135848/1
type: Range
persistent identity: range2135848
display id: range2135848
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 1
end: 654
orientation: inline

BBa_K678006
type: SequenceAnnotation
persistent identity: annotation2135852
display id: annotation2135852
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135852/range2135852/1
component: BBa_K678006

https://synbiohub.org/public/igem/BBa_K678049/annotation2135852/range2135852/1
type: Range
persistent identity: range2135852
display id: range2135852
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 655
end: 1389
orientation: inline

BBa_K678023
type: SequenceAnnotation
persistent identity: annotation2135861
display id: annotation2135861
version: 1
top level: pJEJAM1
location: https://synbiohub.org/public/igem/BBa_K678049/annotation2135861/range2135861/1
component: BBa_K678023

https://synbiohub.org/public/igem/BBa_K678049/annotation2135861/range2135861/1
type: Range
persistent identity: range2135861
display id: range2135861
version: 1
direction: direction/reverse
top level: pJEJAM1
start: 3382
end: 6092
orientation: inline

T7p
type: ComponentDefinition
persistent identity: BBa K133041
display id: BBa_K133041
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter expression plasmid backbone
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:55Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 3352
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Ana Lasic
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: amp promoter
sequence annotation: partial BioBrick prefix (missing EcoRI)
sequence annotation: lacO regulatory site
sequence annotation: pBR322
sequence annotation: BioBrick suffix
sequence annotation: T7 promoter
sequence annotation: kanamycin2 marker
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence: https://synbiohub.org/public/igem/BBa_K133041_sequence/1

amp promoter
type: SequenceAnnotation
persistent identity: annotation1999657
display id: annotation1999657
version: 1
top level: T7p
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999657/range1999657/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999657/range1999657/1
type: Range
persistent identity: range1999657
display id: range1999657
version: 1
top level: T7p
direction: direction/reverse
start: 2969
end: 2997
orientation: inline

partial BioBrick prefix (missing EcoRI)
type: SequenceAnnotation
persistent identity: annotation1999660
display id: annotation1999660
version: 1
top level: T7p
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999660/range1999660/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999660/range1999660/1
type: Range
persistent identity: range1999660
display id: range1999660
version: 1
top level: T7p
direction: direction/forward
start: 3258
end: 3273
orientation: inline

lacO regulatory site
type: SequenceAnnotation
persistent identity: annotation1999659
display id: annotation1999659
version: 1
top level: T7p
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999659/range1999659/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999659/range1999659/1
type: Range
persistent identity: range1999659
display id: range1999659
version: 1
top level: T7p
direction: direction/forward
start: 3191
end: 3218
orientation: inline

pBR322
type: SequenceAnnotation
persistent identity: annotation1999653
display id: annotation1999653
version: 1
top level: T7p
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999653/range1999653/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999653/range1999653/1
type: Range
persistent identity: range1999653
display id: range1999653
version: 1
top level: T7p
direction: direction/reverse
start: 261
end: 880
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1999654
display id: annotation1999654
version: 1
top level: T7p
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999654/range1999654/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999654/range1999654/1
type: Range
persistent identity: range1999654
display id: range1999654
version: 1
top level: T7p
direction: direction/forward
start: 1
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999658
display id: annotation1999658
version: 1
top level: T7p
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999658/range1999658/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999658/range1999658/1
type: Range
persistent identity: range1999658
display id: range1999658
version: 1
top level: T7p
direction: direction/forward
start: 3173
end: 3191
orientation: inline

kanamycin2 marker
type: SequenceAnnotation
persistent identity: annotation1999655
display id: annotation1999655
version: 1
top level: T7p
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999655/range1999655/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999655/range1999655/1
type: Range
persistent identity: range1999655
display id: range1999655
version: 1
top level: T7p
direction: direction/reverse
start: 1097
end: 1912
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1999656
display id: annotation1999656
version: 1
top level: T7p
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999656/range1999656/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999656/range1999656/1
type: Range
persistent identity: range1999656
display id: range1999656
version: 1
top level: T7p
direction: direction/reverse
start: 2067
end: 2927
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

T7start
type: ComponentDefinition
persistent identity: BBa J18912
display id: BBa_J18912
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter + RBS + START
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression vector
owned by: james
owned by: myers
mutable description: Strong T7 promoter and RBS including start codon for protein expression in E. coli or in-vitro. Part is provided in RFC 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * sequence is taken from the commonly used pET3a expression vector * deleted XbaI site between prom. and RBS * T7 promoter sequence (1-19) equals the one in BBa_I712074 * Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J18912_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2065586
display id: annotation2065586
version: 1
top level: T7start
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1

https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
type: Range
persistent identity: range2065586
display id: range2065586
version: 1
top level: T7start
direction: direction/forward
start: 81
end: 83
orientation: inline

BBa_K921000
type: ComponentDefinition
persistent identity: BBa K921000
display id: BBa_K921000
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP + IPTG->PoPs (Mutant I)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
star: false
creator: Eric Pederson
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: Initiation
sequence annotation: LacO
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K921000_sequence/1

Initiation
type: SequenceAnnotation
persistent identity: annotation2205291
display id: annotation2205291
version: 1
top level: BBa_K921000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1
type: Range
persistent identity: range2205291
display id: range2205291
version: 1
top level: BBa_K921000
direction: direction/forward
start: 19
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205290
display id: annotation2205290
version: 1
top level: BBa_K921000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1
type: Range
persistent identity: range2205290
display id: range2205290
version: 1
top level: BBa_K921000
direction: direction/forward
start: 19
end: 46
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2205289
display id: annotation2205289
version: 1
top level: BBa_K921000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1
type: Range
persistent identity: range2205289
display id: range2205289
version: 1
top level: BBa_K921000
direction: direction/forward
start: 1
end: 19
orientation: inline

BBa_K921001
type: ComponentDefinition
persistent identity: BBa K921001
display id: BBa_K921001
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP + IPTG->PoPs (Mutant II)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
star: false
creator: Eric Pederson
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: T7 Promoter
sequence annotation: LacO
sequence annotation: Initiation
sequence: https://synbiohub.org/public/igem/BBa_K921001_sequence/1

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205296
display id: annotation2205296
version: 1
top level: BBa_K921001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1
type: Range
persistent identity: range2205296
display id: range2205296
version: 1
top level: BBa_K921001
direction: direction/forward
start: 1
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205297
display id: annotation2205297
version: 1
top level: BBa_K921001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1
type: Range
persistent identity: range2205297
display id: range2205297
version: 1
top level: BBa_K921001
direction: direction/forward
start: 22
end: 48
orientation: inline

Initiation
type: SequenceAnnotation
persistent identity: annotation2205298
display id: annotation2205298
version: 1
top level: BBa_K921001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1
type: Range
persistent identity: range2205298
display id: range2205298
version: 1
top level: BBa_K921001
direction: direction/forward
start: 18
end: 20
orientation: inline

BBa_K786003
type: ComponentDefinition
persistent identity: BBa K786003
display id: BBa_K786003
version: 1
was generated by: iGEM to SBOL conversion
description: Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin I fusion with HtrII & Tar, which is sensitive to orange light, with sensing spectra covering from 590-650 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRI fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0015
sequence annotation: SRI-HtrI-Tar
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: RBS 1.0
sequence: https://synbiohub.org/public/igem/BBa_K786003_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2199719
display id: annotation2199719
version: 1
top level: BBa_K786003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1
type: Range
persistent identity: range2199719
display id: range2199719
version: 1
direction: direction/forward
top level: BBa_K786003
start: 2029
end: 2158
orientation: inline

SRI-HtrI-Tar
type: SequenceAnnotation
persistent identity: annotation2199706
display id: annotation2199706
version: 1
top level: BBa_K786003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1
type: Range
persistent identity: range2199706
display id: range2199706
version: 1
direction: direction/forward
top level: BBa_K786003
start: 68
end: 1992
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190185
display id: annotation2190185
version: 1
top level: BBa_K786003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1
type: Range
persistent identity: range2190185
display id: range2190185
version: 1
direction: direction/forward
top level: BBa_K786003
start: 1
end: 41
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2190186
display id: annotation2190186
version: 1
top level: BBa_K786003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1
type: Range
persistent identity: range2190186
display id: range2190186
version: 1
direction: direction/forward
top level: BBa_K786003
start: 42
end: 61
orientation: inline

T7RSR
type: ComponentDefinition
persistent identity: BBa K786031
display id: BBa_K786031
version: 1
was generated by: iGEM to SBOL conversion
description: T7promoter-RSR-T7teminator
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:22Z
mutable provenance: The 'repeat' sequence in CRISPR/cas system of E.coli strain K12.
owned by: james
owned by: myers
mutable description: It is a repeat-spacer-repeat region designed according to E.coli's CRISPR/Cas system. It also includes a T7 promoter and T7 terminator. THe spacer is designed to target on a segment of non-coding-sequence in E.coli's genome. This biobrick can be transformed into E.coli cells to hopefully construct a suicide system with E.coli's CRISPR/Cas system.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1041_
m user id: 0
owner id: 14280
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The repeat-spacer-repeat region design should be consistent with the operating principles of E.coli's CRISPR/cas system.
star: false
creator: Kaiyin Feng
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: T7-promoter
sequence annotation: repeat-spacer-repeat region
sequence annotation: T7-terminator
sequence: https://synbiohub.org/public/igem/BBa_K786031_sequence/1

T7-promoter
type: SequenceAnnotation
persistent identity: annotation2203441
display id: annotation2203441
version: 1
top level: T7RSR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786031/annotation2203441/range2203441/1

https://synbiohub.org/public/igem/BBa_K786031/annotation2203441/range2203441/1
type: Range
persistent identity: range2203441
display id: range2203441
version: 1
top level: T7RSR
direction: direction/forward
start: 1
end: 23
orientation: inline

repeat-spacer-repeat region
type: SequenceAnnotation
persistent identity: annotation2203442
display id: annotation2203442
version: 1
top level: T7RSR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786031/annotation2203442/range2203442/1

https://synbiohub.org/public/igem/BBa_K786031/annotation2203442/range2203442/1
type: Range
persistent identity: range2203442
display id: range2203442
version: 1
top level: T7RSR
direction: direction/forward
start: 31
end: 803
orientation: inline

T7-terminator
type: SequenceAnnotation
persistent identity: annotation2203443
display id: annotation2203443
version: 1
top level: T7RSR
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K786031/annotation2203443/range2203443/1

https://synbiohub.org/public/igem/BBa_K786031/annotation2203443/range2203443/1
type: Range
persistent identity: range2203443
display id: range2203443
version: 1
top level: T7RSR
direction: direction/forward
start: 812
end: 956
orientation: inline

BBa_K1680026
type: ComponentDefinition
persistent identity: BBa K1680026
display id: BBa_K1680026
version: 1
was generated by: iGEM to SBOL conversion
description: pETUE
creator: Katharina Sporbeck
created: 2015-09-17T11:00:00Z
modified: 2015-10-28T03:58:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Expression Plasmid coding for His-tag, also containing a thrombine cleavage site between coding region and Histag, so that His-tag can be cleaved off. Vecotr opening with PstI/XmaI, clone part into it with PstI/NgoMIV. Ampicillin resistance.
mutable notes: very strong RBS 6xHis-Tag Thromin-cleavage-site NgoMIV-compatible restriction site for in-frame insertion
mutable provenance: pET-Vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: tet promoter
sequence annotation: thrombin cleavage site
sequence annotation: T7 promoter
sequence annotation: Lac operator
sequence annotation: RBS.1 strong
sequence annotation: T7 terminator
sequence annotation: rrnB T1 terminator
sequence annotation: lac operator
sequence annotation: ori
sequence annotation: f1 ori
sequence annotation: AmpR
sequence annotation: 6x His-tag
sequence: https://synbiohub.org/public/igem/BBa_K1680026_sequence/1

tet promoter
type: SequenceAnnotation
persistent identity: annotation2472238
display id: annotation2472238
version: 1
top level: BBa_K1680026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472238/range2472238/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472238/range2472238/1
type: Range
persistent identity: range2472238
display id: range2472238
version: 1
direction: direction/reverse
top level: BBa_K1680026
start: 212
end: 240
orientation: inline

thrombin cleavage site
type: SequenceAnnotation
persistent identity: annotation2472237
display id: annotation2472237
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472237/range2472237/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472237/range2472237/1
type: Range
persistent identity: range2472237
display id: range2472237
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 104
end: 121
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2472193
display id: annotation2472193
version: 1
top level: BBa_K1680026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472193/range2472193/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472193/range2472193/1
type: Range
persistent identity: range2472193
display id: range2472193
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 1
end: 19
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2472194
display id: annotation2472194
version: 1
top level: BBa_K1680026
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472194/range2472194/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472194/range2472194/1
type: Range
persistent identity: range2472194
display id: range2472194
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 20
end: 44
orientation: inline

RBS.1 strong
type: SequenceAnnotation
persistent identity: annotation2472195
display id: annotation2472195
version: 1
top level: BBa_K1680026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472195/range2472195/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472195/range2472195/1
type: Range
persistent identity: range2472195
display id: range2472195
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 53
end: 67
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2472260
display id: annotation2472260
version: 1
top level: BBa_K1680026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472260/range2472260/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472260/range2472260/1
type: Range
persistent identity: range2472260
display id: range2472260
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 683
end: 730
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2472239
display id: annotation2472239
version: 1
top level: BBa_K1680026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472239/range2472239/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472239/range2472239/1
type: Range
persistent identity: range2472239
display id: range2472239
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 410
end: 496
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2472328
display id: annotation2472328
version: 1
top level: BBa_K1680026
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472328/range2472328/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472328/range2472328/1
type: Range
persistent identity: range2472328
display id: range2472328
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 3277
end: 3296
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2472305
display id: annotation2472305
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472305/range2472305/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472305/range2472305/1
type: Range
persistent identity: range2472305
display id: range2472305
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 2290
end: 2878
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation2472261
display id: annotation2472261
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472261/range2472261/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472261/range2472261/1
type: Range
persistent identity: range2472261
display id: range2472261
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 767
end: 1222
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2472289
display id: annotation2472289
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472289/range2472289/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472289/range2472289/1
type: Range
persistent identity: range2472289
display id: range2472289
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 1337
end: 2197
orientation: inline

6x His-tag
type: SequenceAnnotation
persistent identity: annotation2472216
display id: annotation2472216
version: 1
top level: BBa_K1680026
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472216/range2472216/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472216/range2472216/1
type: Range
persistent identity: range2472216
display id: range2472216
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 77
end: 94
orientation: inline

BBa_K819008
type: ComponentDefinition
persistent identity: BBa K819008
display id: BBa_K819008
version: 1
was generated by: iGEM to SBOL conversion
description: Lux Operon under T7 promoter
creator: ZHANG Zidong
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: luxABCDE gene ligated to the rear of T7 promoter. When transformed into BL21(DE3) E.coli strain, add different amount IPTG to reach different final concentration of IPTG in the medium can induce different level of Lux ligt emission. No substrate is needed.
mutable notes: luxABCDE gene ligated to the rear of T7 promoter. The original Ara and P-bad were replaced with T7 promoter, which enables its usage in our project.
mutable provenance: T7 promoter and LuxCDABE with their RBS are from Partsregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K819008
sequence annotation: LuxCDABEG with corresponding six RBS(from K325909)
sequence: https://synbiohub.org/public/igem/BBa_K819008_sequence/1

BBa_K819008
type: SequenceAnnotation
persistent identity: annotation2197308
display id: annotation2197308
version: 1
top level: BBa_K819008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819008/annotation2197308/range2197308/1

https://synbiohub.org/public/igem/BBa_K819008/annotation2197308/range2197308/1
type: Range
persistent identity: range2197308
display id: range2197308
version: 1
direction: direction/forward
top level: BBa_K819008
start: 1
end: 46
orientation: inline

LuxCDABEG with corresponding six RBS(from K325909)
type: SequenceAnnotation
persistent identity: annotation2197309
display id: annotation2197309
version: 1
top level: BBa_K819008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K819008/annotation2197309/range2197309/1

https://synbiohub.org/public/igem/BBa_K819008/annotation2197309/range2197309/1
type: Range
persistent identity: range2197309
display id: range2197309
version: 1
direction: direction/forward
top level: BBa_K819008
start: 53
end: 6480
orientation: inline

T7stop
type: ComponentDefinition
persistent identity: BBa J18913
display id: BBa_J18913
version: 1
was generated by: iGEM to SBOL conversion
description: T7 terminator (incl. STOP)
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: pET3a expression plasmid
owned by: james
owned by: myers
mutable description: T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J18913_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2065587
display id: annotation2065587
version: 1
top level: T7stop
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1

https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
type: Range
persistent identity: range2065587
display id: range2065587
version: 1
top level: T7stop
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K2127001
type: ComponentDefinition
persistent identity: BBa K2127001
display id: BBa_K2127001
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible High Expression His-Tagged Photosystem II CP47 subunit
creator: Nafaa Haddou
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:45:18Z
discontinued: false
dominant: false
group u list: _2596_
m user id: 0
m user id: 32415
owner id: 32415
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TFBS plac LacO CP47his
mutable notes: synthesized
mutable provenance: Synchocisytys
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0030
component: BBa_K2127004
component: BBa_K2127007
component: BBa_K2127006
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_K2127006
sequence annotation: BBa_K2127004
sequence annotation: BBa_K2127007
sequence: https://synbiohub.org/public/igem/BBa_K2127001_sequence/1

BBa_B0030
type: Component
persistent identity: component2527075
display id: component2527075
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K2127004
type: Component
persistent identity: component2527081
display id: component2527081
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127004/1

BBa_K2127007
type: Component
persistent identity: component2527082
display id: component2527082
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127007/1

BBa_K2127006
type: Component
persistent identity: component2527066
display id: component2527066
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127006/1

BBa_R0010
type: Component
persistent identity: component2527067
display id: component2527067
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2527067
display id: annotation2527067
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527067/range2527067/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527067/range2527067/1
type: Range
persistent identity: range2527067
display id: range2527067
version: 1
direction: direction/forward
top level: BBa_K2127001
start: 186
end: 385
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2527075
display id: annotation2527075
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527075/range2527075/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527075/range2527075/1
type: Range
persistent identity: range2527075
display id: range2527075
version: 1
direction: direction/forward
top level: BBa_K2127001
start: 386
end: 400
orientation: inline

BBa_K2127006
type: SequenceAnnotation
persistent identity: annotation2527066
display id: annotation2527066
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527066/range2527066/1
component: BBa_K2127006

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527066/range2527066/1
type: Range
persistent identity: range2527066
display id: range2527066
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 1
end: 185
orientation: inline

BBa_K2127004
type: SequenceAnnotation
persistent identity: annotation2527081
display id: annotation2527081
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527081/range2527081/1
component: BBa_K2127004

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527081/range2527081/1
type: Range
persistent identity: range2527081
display id: range2527081
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 401
end: 1948
orientation: inline

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2527082
display id: annotation2527082
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527082/range2527082/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527082/range2527082/1
type: Range
persistent identity: range2527082
display id: range2527082
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 1949
end: 2071
orientation: inline

pEX
type: ComponentDefinition
persistent identity: BBa K243033
display id: BBa_K243033
version: 1
was generated by: iGEM to SBOL conversion
description: pEX
creator: Freiburg Bioware09
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is our expression vector. The plasmid has the restriction sites XbaI NgoMIV_part_AgeI_SpeI_NotI_PstI
mutable notes: Noine.
mutable provenance: By adding additional restriction sites in pMAL we create our expression vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_K243033_sequence/1

BBa_K786002
type: ComponentDefinition
persistent identity: BBa K786002
display id: BBa_K786002
version: 1
was generated by: iGEM to SBOL conversion
description: Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin II fusion with HtrII & Tar, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: RBS 1.0
sequence annotation: HindIII for N-terminal fusin
sequence annotation: BamHI for C-terminal fusion
sequence annotation: SRII-HtrII-Tar
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K786002_sequence/1

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199689
display id: annotation2199689
version: 1
top level: BBa_K786002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1
type: Range
persistent identity: range2199689
display id: range2199689
version: 1
direction: direction/forward
top level: BBa_K786002
start: 1
end: 41
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2199690
display id: annotation2199690
version: 1
top level: BBa_K786002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1
type: Range
persistent identity: range2199690
display id: range2199690
version: 1
direction: direction/forward
top level: BBa_K786002
start: 42
end: 61
orientation: inline

HindIII for N-terminal fusin
type: SequenceAnnotation
persistent identity: annotation2199691
display id: annotation2199691
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1
type: Range
persistent identity: range2199691
display id: range2199691
version: 1
direction: direction/forward
top level: BBa_K786002
start: 62
end: 67
orientation: inline

BamHI for C-terminal fusion
type: SequenceAnnotation
persistent identity: annotation2199693
display id: annotation2199693
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1
type: Range
persistent identity: range2199693
display id: range2199693
version: 1
direction: direction/forward
top level: BBa_K786002
start: 785
end: 790
orientation: inline

SRII-HtrII-Tar
type: SequenceAnnotation
persistent identity: annotation2199692
display id: annotation2199692
version: 1
top level: BBa_K786002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1
type: Range
persistent identity: range2199692
display id: range2199692
version: 1
direction: direction/forward
top level: BBa_K786002
start: 68
end: 2083
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2199694
display id: annotation2199694
version: 1
top level: BBa_K786002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1
type: Range
persistent identity: range2199694
display id: range2199694
version: 1
direction: direction/forward
top level: BBa_K786002
start: 2117
end: 2245
orientation: inline

BBa_K2074133
type: ComponentDefinition
persistent identity: BBa K2074133
display id: BBa_K2074133
version: 1
was generated by: iGEM to SBOL conversion
description: pChlamy_3
creator: Hao Feng
created: 2016-10-10T11:00:00Z
modified: 2016-10-14T06:54:59Z
discontinued: true
dominant: false
experience: experience/None
group u list: _2542_
m user id: 30527
owner id: 30527
owning group id: 9
status: status/Deleted
bookmark: false
mutable description: 1
mutable notes: 3
mutable provenance: 2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K2074133_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2491860
display id: annotation2491860
version: 1
top level: BBa_K2074133
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2074133/annotation2491860/range2491860/1

https://synbiohub.org/public/igem/BBa_K2074133/annotation2491860/range2491860/1
type: Range
persistent identity: range2491860
display id: range2491860
version: 1
direction: direction/forward
top level: BBa_K2074133
start: 574
end: 718
orientation: inline

BBa_K559010
type: ComponentDefinition
persistent identity: BBa K559010
display id: BBa_K559010
version: 1
was generated by: iGEM to SBOL conversion
description: Halorhodopsin complete system
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: Halorhodopsin comes from part BBa_K559000, which is come from genomic sequence of N.pharaonis (genebank ID:J05199.1)
owned by: james
owned by: myers
mutable description: Halorhodopsin gene allow chloride ion to be pumped in by the level of light intensity. Halorhodopsin gene is under regulation by constitute T7 promoter to allow protein coding to be maximized, and the bidirectional terminator is added for high efficiency termination of both forward and backward transcription
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The double terminator wass tested to stop effectively on the transcription of both forward and backward transcription to make our protein generator be a controlled system
star: false
creator: Jacky, Fong Chuen, Loo
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K559001
component: BBa_B0014
sequence annotation: BBa_K559001
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K559010_sequence/1

BBa_K559001
type: Component
persistent identity: component2177574
display id: component2177574
version: 1
top level: BBa_K559010
access: public
definition: BBa_K559001

BBa_B0014
type: Component
persistent identity: component2177581
display id: component2177581
version: 1
top level: BBa_K559010
access: public
definition: BBa_B0014

BBa_K559001
type: SequenceAnnotation
persistent identity: annotation2177574
display id: annotation2177574
version: 1
top level: BBa_K559010
location: https://synbiohub.org/public/igem/BBa_K559010/annotation2177574/range2177574/1
component: BBa_K559001

https://synbiohub.org/public/igem/BBa_K559010/annotation2177574/range2177574/1
type: Range
persistent identity: range2177574
display id: range2177574
version: 1
top level: BBa_K559010
direction: direction/reverse
start: 1
end: 728
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2177581
display id: annotation2177581
version: 1
top level: BBa_K559010
location: https://synbiohub.org/public/igem/BBa_K559010/annotation2177581/range2177581/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K559010/annotation2177581/range2177581/1
type: Range
persistent identity: range2177581
display id: range2177581
version: 1
top level: BBa_K559010
direction: direction/reverse
start: 737
end: 831
orientation: inline

BBa_K533001
type: ComponentDefinition
persistent identity: BBa K533001
display id: BBa_K533001
version: 1
was generated by: iGEM to SBOL conversion
description: OmpA-SH3
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
mutable provenance: OmpA is derived from the genomic DNA of BL21 (DE3) E. Coli strain while SH3 is from human Grb2.
owned by: james
owned by: myers
mutable description: This part contains the N-terminal 180 amino acids of OmpA fused to SH3 domain in human Grb2 protein. The N-terminal of OmpA allows the moiety fused to it to be presented on to the outer membrane of E. Coli. SH3 domain allows for binding to multi-proline peptide in human cells, which functions as a crucial adaptor module in signaling. In our part, this is specially for binding to the proteins for transportation by E. Coli. All the coding sequence is under T7 promoter and lac repressor binding site. The parts is most efficiently presented onto the membrane when induced at 0.1mM IPTG at 18 centigrade in BL21 E. Coli strain.
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: High expression efficiency as well as inducible expression is most desirable when considering a coding sequence.
star: false
creator: ZHANG Yunxiao
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: SH3
sequence annotation: OmpA
sequence: https://synbiohub.org/public/igem/BBa_K533001_sequence/1

lac operator
type: SequenceAnnotation
persistent identity: annotation2150406
display id: annotation2150406
version: 1
top level: BBa_K533001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150406/range2150406/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150406/range2150406/1
type: Range
persistent identity: range2150406
display id: range2150406
version: 1
top level: BBa_K533001
direction: direction/forward
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2150407
display id: annotation2150407
version: 1
top level: BBa_K533001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150407/range2150407/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150407/range2150407/1
type: Range
persistent identity: range2150407
display id: range2150407
version: 1
top level: BBa_K533001
direction: direction/forward
start: 1
end: 18
orientation: inline

SH3
type: SequenceAnnotation
persistent identity: annotation2150409
display id: annotation2150409
version: 1
top level: BBa_K533001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150409/range2150409/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150409/range2150409/1
type: Range
persistent identity: range2150409
display id: range2150409
version: 1
top level: BBa_K533001
direction: direction/forward
start: 633
end: 815
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2150408
display id: annotation2150408
version: 1
top level: BBa_K533001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150408/range2150408/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150408/range2150408/1
type: Range
persistent identity: range2150408
display id: range2150408
version: 1
top level: BBa_K533001
direction: direction/forward
start: 87
end: 632
orientation: inline

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18913_sequence/1
type: Sequence
persistent identity: BBa J18913 sequence
display id: BBa_J18913_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K921002_sequence/1
type: Sequence
persistent identity: BBa K921002 sequence
display id: BBa_K921002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatccgactcactaaagagagaattgtgagcggataacaattccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K559001_sequence/1
type: Sequence
persistent identity: BBa K559001 sequence
display id: BBa_K559001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactataggggaattgtgagcggataacaattccccaataattttgtttaactttaagaagaagatatagtactagagatgactgagacattgccaccggtaacggaatcggctgttgcgctacaggcggaggtgacccagagggagctgttcgagttcgttctcaacgaccccctcctcgccagttcgctgtatattaatatcgcactggcagggctgtcgatactgcttttcgtgttcatgacgcgcggactcgacgacccacgggcgaaactcatcgccgtttcgacgattttggtgccggtggtctctatcgcgagctacaccggccttgcatcggggctcaccatcagcgtcctcgagatgccagccggccacttcgccgaggggtcctcggtgatgctcggcggcgaagaggtagacggcgtcgtgacgatgtggggccgctatctgacgtgggccctttcgacaccgatgatactgctggcgcttgggctgcttgctggctctaacgccacgaagctctttaccgccatcaccttcgacatcgcgatgtgtgtcaccggcctcgcagccgcgctgacgacctcttcgcacctgatgcggtggttctggtacgccatcagttgtgcgtgtttcctcgtcgtcctctacatcctgctcgtcgagtgggcacaggacgccaaggctgccggtactgcggatatgttcaatacgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K559010_sequence/1
type: Sequence
persistent identity: BBa K559010 sequence
display id: BBa_K559010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactataggggaattgtgagcggataacaattccccaataattttgtttaactttaagaagaagatatagtactagagatgactgagacattgccaccggtaacggaatcggctgttgcgctacaggcggaggtgacccagagggagctgttcgagttcgttctcaacgaccccctcctcgccagttcgctgtatattaatatcgcactggcagggctgtcgatactgcttttcgtgttcatgacgcgcggactcgacgacccacgggcgaaactcatcgccgtttcgacgattttggtgccggtggtctctatcgcgagctacaccggccttgcatcggggctcaccatcagcgtcctcgagatgccagccggccacttcgccgaggggtcctcggtgatgctcggcggcgaagaggtagacggcgtcgtgacgatgtggggccgctatctgacgtgggccctttcgacaccgatgatactgctggcgcttgggctgcttgctggctctaacgccacgaagctctttaccgccatcaccttcgacatcgcgatgtgtgtcaccggcctcgcagccgcgctgacgacctcttcgcacctgatgcggtggttctggtacgccatcagttgtgcgtgtttcctcgtcgtcctctacatcctgctcgtcgagtgggcacaggacgccaaggctgccggtactgcggatatgttcaatacgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K133041_sequence/1
type: Sequence
persistent identity: BBa K133041 sequence
display id: BBa_K133041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K921001_sequence/1
type: Sequence
persistent identity: BBa K921001 sequence
display id: BBa_K921001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactacagggcggaattgtgagcggataacaattccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K533001_sequence/1
type: Sequence
persistent identity: BBa K533001 sequence
display id: BBa_K533001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactataggggaattgtgagcggataacaattccccctagtaataattttgtttaactttaagaaggagatataccatggcaatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccgctccgaaagataacacctggtacactggtgctaaactgggctggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttggtggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacatggaagccatcgccaaatatgacttcaaagctactgcggacgacgagctgagcttcaaaaggggggacatcctcaaggttttgaacgaagaatgtgatcagaactggtacaaggcagagcttaatggaaaagacggcttcattcccaagaactacatagaaatgaaaccacatccgtggtaggatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1428000_sequence/1
type: Sequence
persistent identity: BBa K1428000 sequence
display id: BBa_K1428000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaataattttgtttaactttaagaaggagatataccatggatcagaaacagattgaagaaattgtgcgcagcgtgatggcgagcatgggccagacgggctcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K533002_sequence/1
type: Sequence
persistent identity: BBa K533002 sequence
display id: BBa_K533002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactataggggaattgtgagcggataacaattccccctagaaataattttgtttaactttaagaaggagatataccatggcaatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccgctccgaaagataacacctggtacactggtgctaaactgggctggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttggtggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacagccaggtgagccagaactaccccatcgtgcagaacctccaaatggaagccatcgccaaatatgacttcaaagctactgctgacgacgagctgagcttcaaaaggggggacatcctcaaggttttgaacgaagaatgtgatcagaactggtacaaggcagagcttaatggaaaagacggcttcattcccaagaactacatagaaatgaaaccacatccgtggtaggatccggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K533003_sequence/1
type: Sequence
persistent identity: BBa K533003 sequence
display id: BBa_K533003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactataggggaattgtgagcggataacaattccccctagaaataattttgtttaactttaagaaggagatataccatggcaatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccgctccgaaagataacacctggtacactggtgctaaactgggctggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttggtggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacatgcctcagatcactctttggcagcgacccctcgtcacaataaagataggggggcaattaaaggaagctctattagatacaggagcagatgatacagtattagaagaaatgaatttgccaggaagatggaaaccaaaaatgatagggggaattggaggttttatcaaagtaagacagtatgatcagatactcatagaaatctgcggacataaagctataggtacagtattagtaggacctacacctgtcaacataattggaagaaatctgttgactcagattggctgcactttaaatttttagggatccggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K921000_sequence/1
type: Sequence
persistent identity: BBa K921000 sequence
display id: BBa_K921000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatgcgactcactataggacaattgtgggcggacaacaattccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1614000_sequence/1
type: Sequence
persistent identity: BBa K1614000 sequence
display id: BBa_K1614000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18912_sequence/1
type: Sequence
persistent identity: BBa J18912 sequence
display id: BBa_J18912_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaccacaacggtttccctctagcaataattttgtttaactttaagaaggagatatacatatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K786031_sequence/1
type: Sequence
persistent identity: BBa K786031 sequence
display id: BBa_K786031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtccgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcgggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgcccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtatactatagggagatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactatagggagatactagagagttccccgcgccagcggggataaaccgcgtgtgtaagtttgcaattccgtttgttgtatgagttccccgcgccagcggggataaaccgtaatactagagagttcccgcgccagcggggataaaccgtaatactagagggagatactagagagttccccgcgcagcgggataaaccgtaatactagagtggccggctagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z