//plasmidbackbone/assembly
type: Collection
persistent identity: plasmidbackbone assembly
display id: plasmidbackbone_assembly
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K864013
member: BBa_J179000
member: pSB8K15
member: pSB4C1
member: pSB4A5
member: BBa_J64100
member: pSB2K4
member: BBa_K864006
member: pSB8A15FRT
member: pSB1C3
member: BBa_K864015
member: pSB4K5
member: pSB4T5
member: pSB4C50
member: BBa_K864011
member: BBa_K823055
member: pSB1AT3
member: BBa_K864010
member: pSB1K3
member: pSB3K1
member: BBa_K864003
member: pSB8A15
member: pSB8S15FRT
member: pSB1A30
member: K592200
member: pSB4C5
member: pSB8C15
member: BBa_K864004
member: pSB8K15FRT
member: pSB1A7
member: pSBBs1C
member: pSB1A3
member: BBa_K864000
member: pSB8C15FRT
member: BBa_K864005
member: BBa_K864014
member: BBa_K864012
member: pSB1T3
member: pSB3K3
member: pSB8S15
member: pSB1AK3
member: pSB4K50
member: pSB2K3
member: BBa_K864002
member: pSB3C5
member: BBa_K864007
member: pSB6A1
member: pSB4A50
member: BBa_K864008
member: pSB3K5
member: BBa_K864001
member: pSB3T5
member: BBa_K864009
member: pSB1AC3

pSB1A3
type: ComponentDefinition
persistent identity: pSB1A3
display id: pSB1A3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Reshma Shetty & Tom Knight
created: 2004-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 52
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1A3-1 is a high copy number plasmid carrying ampicillin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). pSB1A3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region. A fully annotated Genbank file is attached below.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator stem loop
sequence annotation: VF2
sequence annotation: suffix
sequence annotation: end of terminator
sequence annotation: ORI
sequence annotation: E. coli his operon terminator
sequence annotation: rep(pMB1) (rev)
sequence annotation: prefix
sequence annotation: ampR (rev)
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/pSB1A3_sequence/1

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2119978
display id: annotation2119978
version: 1
top level: pSB1A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119978/range2119978/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119978/range2119978/1
type: Range
persistent identity: range2119978
display id: range2119978
version: 1
direction: direction/reverse
top level: pSB1A3
start: 2107
end: 2126
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2119976
display id: annotation2119976
version: 1
top level: pSB1A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119976/range2119976/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119976/range2119976/1
type: Range
persistent identity: range2119976
display id: range2119976
version: 1
direction: direction/forward
top level: pSB1A3
start: 2018
end: 2037
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2119969
display id: annotation2119969
version: 1
top level: pSB1A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119969/range2119969/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119969/range2119969/1
type: Range
persistent identity: range2119969
display id: range2119969
version: 1
direction: direction/forward
top level: pSB1A3
start: 1
end: 21
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2119977
display id: annotation2119977
version: 1
top level: pSB1A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119977/range2119977/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119977/range2119977/1
type: Range
persistent identity: range2119977
display id: range2119977
version: 1
direction: direction/reverse
top level: pSB1A3
start: 2094
end: 2097
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2119971
display id: annotation2119971
version: 1
top level: pSB1A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119971/range2119971/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119971/range2119971/1
type: Range
persistent identity: range2119971
display id: range2119971
version: 1
direction: direction/forward
top level: pSB1A3
start: 30
end: 64
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2119974
display id: annotation2119974
version: 1
top level: pSB1A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119974/range2119974/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119974/range2119974/1
type: Range
persistent identity: range2119974
display id: range2119974
version: 1
direction: direction/forward
top level: pSB1A3
start: 276
end: 276
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2119970
display id: annotation2119970
version: 1
top level: pSB1A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119970/range2119970/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119970/range2119970/1
type: Range
persistent identity: range2119970
display id: range2119970
version: 1
direction: direction/forward
top level: pSB1A3
start: 22
end: 93
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2119973
display id: annotation2119973
version: 1
top level: pSB1A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119973/range2119973/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119973/range2119973/1
type: Range
persistent identity: range2119973
display id: range2119973
version: 1
direction: direction/reverse
top level: pSB1A3
start: 261
end: 875
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2119979
display id: annotation2119979
version: 1
top level: pSB1A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119979/range2119979/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119979/range2119979/1
type: Range
persistent identity: range2119979
display id: range2119979
version: 1
direction: direction/forward
top level: pSB1A3
start: 2136
end: 2157
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation2119975
display id: annotation2119975
version: 1
top level: pSB1A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119975/range2119975/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119975/range2119975/1
type: Range
persistent identity: range2119975
display id: range2119975
version: 1
direction: direction/reverse
top level: pSB1A3
start: 1035
end: 1895
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2119972
display id: annotation2119972
version: 1
top level: pSB1A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119972/range2119972/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119972/range2119972/1
type: Range
persistent identity: range2119972
display id: range2119972
version: 1
direction: direction/reverse
top level: pSB1A3
start: 157
end: 176
orientation: inline

BBa_K864006
type: ComponentDefinition
persistent identity: BBa K864006
display id: BBa_K864006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864006_sequence/1

pSB4A50
type: ComponentDefinition
persistent identity: BBa K1362095
display id: BBa_K1362095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A50: Low copy BioBrick cloning/expression backbone carrying Amp resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00102
component: BBa_K1362463
component: BBa_B0042
component: BBa_B0044
component: BBa_B0062
component: BBa_G0002
component: BBa_G0003
component: BBa_B0053
component: BBa_P1002
component: BBa_G00100
component: BBa_G0004
component: BBa_G00001
component: BBa_B0054
component: BBa_G00000
component: BBa_I50042
component: BBa_B0055
component: BBa_B0043
sequence annotation: BBa_B0055
sequence annotation: BBa_P1002
sequence annotation: BBa_B0044
sequence annotation: BBa_B0062
sequence annotation: BBa_G0002
sequence annotation: BBa_K1362463
sequence annotation: BBa_B0042
sequence annotation: BBa_B0043
sequence annotation: BBa_G0003
sequence annotation: BBa_G00102
sequence annotation: BBa_G0004
sequence annotation: BBa_G00100
sequence annotation: BBa_B0053
sequence annotation: BBa_B0054
sequence annotation: BBa_I50042
sequence annotation: BBa_G00000
sequence annotation: BBa_G00001
sequence: https://synbiohub.org/public/igem/BBa_K1362095_sequence/1

BBa_G00102
type: Component
persistent identity: component2409781
display id: component2409781
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_K1362463
type: Component
persistent identity: component2409842
display id: component2409842
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_B0042
type: Component
persistent identity: component2409776
display id: component2409776
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component2409769
display id: component2409769
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409783
display id: component2409783
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0002
type: Component
persistent identity: component2409813
display id: component2409813
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0003
type: Component
persistent identity: component2409784
display id: component2409784
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0042
type: Component
persistent identity: component2409833
display id: component2409833
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0053
type: Component
persistent identity: component2409821
display id: component2409821
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_P1002
type: Component
persistent identity: component2409818
display id: component2409818
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_G00100
type: Component
persistent identity: component2409823
display id: component2409823
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G0004
type: Component
persistent identity: component2409819
display id: component2409819
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00001
type: Component
persistent identity: component2409764
display id: component2409764
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0054
type: Component
persistent identity: component2409779
display id: component2409779
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00000
type: Component
persistent identity: component2409847
display id: component2409847
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_I50042
type: Component
persistent identity: component2409812
display id: component2409812
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0055
type: Component
persistent identity: component2409826
display id: component2409826
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0043
type: Component
persistent identity: component2409838
display id: component2409838
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409826
display id: annotation2409826
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
type: Range
persistent identity: range2409826
display id: range2409826
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3271
end: 3348
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2409818
display id: annotation2409818
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
component: BBa_P1002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
type: Range
persistent identity: range2409818
display id: range2409818
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2229
end: 3171
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409769
display id: annotation2409769
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
type: Range
persistent identity: range2409769
display id: range2409769
version: 1
direction: direction/reverse
top level: pSB4A50
start: 22
end: 34
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409783
display id: annotation2409783
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
type: Range
persistent identity: range2409783
display id: range2409783
version: 1
direction: direction/reverse
top level: pSB4A50
start: 136
end: 176
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409813
display id: annotation2409813
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
type: Range
persistent identity: range2409813
display id: range2409813
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2221
end: 2228
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409842
display id: annotation2409842
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
type: Range
persistent identity: range2409842
display id: range2409842
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3374
end: 3424
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409776
display id: annotation2409776
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
type: Range
persistent identity: range2409776
display id: range2409776
version: 1
direction: direction/reverse
top level: pSB4A50
start: 35
end: 46
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409838
display id: annotation2409838
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
type: Range
persistent identity: range2409838
display id: range2409838
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3361
end: 3373
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409784
display id: annotation2409784
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
type: Range
persistent identity: range2409784
display id: range2409784
version: 1
direction: direction/reverse
top level: pSB4A50
start: 177
end: 183
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409833
display id: annotation2409833
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
type: Range
persistent identity: range2409833
display id: range2409833
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3349
end: 3360
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409781
display id: annotation2409781
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
type: Range
persistent identity: range2409781
display id: range2409781
version: 1
direction: direction/reverse
top level: pSB4A50
start: 116
end: 135
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409819
display id: annotation2409819
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
type: Range
persistent identity: range2409819
display id: range2409819
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3172
end: 3178
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409823
display id: annotation2409823
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
type: Range
persistent identity: range2409823
display id: range2409823
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3251
end: 3270
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409821
display id: annotation2409821
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
type: Range
persistent identity: range2409821
display id: range2409821
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3179
end: 3250
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409779
display id: annotation2409779
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
type: Range
persistent identity: range2409779
display id: range2409779
version: 1
direction: direction/reverse
top level: pSB4A50
start: 47
end: 115
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409812
display id: annotation2409812
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
type: Range
persistent identity: range2409812
display id: range2409812
version: 1
direction: direction/reverse
top level: pSB4A50
start: 184
end: 2220
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409847
display id: annotation2409847
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
type: Range
persistent identity: range2409847
display id: range2409847
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3425
end: 3446
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409764
display id: annotation2409764
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
type: Range
persistent identity: range2409764
display id: range2409764
version: 1
direction: direction/reverse
top level: pSB4A50
start: 1
end: 21
orientation: inline

pSB1C3
type: ComponentDefinition
persistent identity: pSB1C3
display id: pSB1C3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Austin Che
created: 2008-09-07T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.
mutable notes: Eliminated ampicillin resistance.
mutable provenance: This part was derived from pSB1AC3-P1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Stem loop
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: T0 terminator
sequence annotation: BioBrick prefix
sequence annotation: ORI
sequence annotation: E. coli his operon terminator
sequence annotation: end of terminator
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: BioBrick suffix
sequence annotation: rep (pMB1)
sequence annotation: Chloramphenicol resistance
sequence: https://synbiohub.org/public/igem/pSB1C3_sequence/1

Stem loop
type: SequenceAnnotation
persistent identity: annotation2119040
display id: annotation2119040
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119040/range2119040/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119040/range2119040/1
type: Range
persistent identity: range2119040
display id: range2119040
version: 1
direction: direction/forward
top level: pSB1C3
start: 30
end: 64
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation2119046
display id: annotation2119046
version: 1
top level: pSB1C3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119046/range2119046/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119046/range2119046/1
type: Range
persistent identity: range2119046
display id: range2119046
version: 1
direction: direction/forward
top level: pSB1C3
start: 1933
end: 1952
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2119044
display id: annotation2119044
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119044/range2119044/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119044/range2119044/1
type: Range
persistent identity: range2119044
display id: range2119044
version: 1
direction: direction/forward
top level: pSB1C3
start: 1042
end: 1147
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2119049
display id: annotation2119049
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119049/range2119049/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119049/range2119049/1
type: Range
persistent identity: range2119049
display id: range2119049
version: 1
direction: direction/forward
top level: pSB1C3
start: 2051
end: 2072
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2119043
display id: annotation2119043
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119043/range2119043/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119043/range2119043/1
type: Range
persistent identity: range2119043
display id: range2119043
version: 1
direction: direction/forward
top level: pSB1C3
start: 276
end: 276
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2119039
display id: annotation2119039
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119039/range2119039/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119039/range2119039/1
type: Range
persistent identity: range2119039
display id: range2119039
version: 1
direction: direction/forward
top level: pSB1C3
start: 22
end: 93
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2119047
display id: annotation2119047
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119047/range2119047/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119047/range2119047/1
type: Range
persistent identity: range2119047
display id: range2119047
version: 1
direction: direction/reverse
top level: pSB1C3
start: 2009
end: 2012
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2119048
display id: annotation2119048
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119048/range2119048/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119048/range2119048/1
type: Range
persistent identity: range2119048
display id: range2119048
version: 1
direction: direction/reverse
top level: pSB1C3
start: 2022
end: 2041
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation2119041
display id: annotation2119041
version: 1
top level: pSB1C3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119041/range2119041/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119041/range2119041/1
type: Range
persistent identity: range2119041
display id: range2119041
version: 1
direction: direction/reverse
top level: pSB1C3
start: 157
end: 176
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2119038
display id: annotation2119038
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119038/range2119038/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119038/range2119038/1
type: Range
persistent identity: range2119038
display id: range2119038
version: 1
direction: direction/forward
top level: pSB1C3
start: 1
end: 21
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2119042
display id: annotation2119042
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119042/range2119042/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119042/range2119042/1
type: Range
persistent identity: range2119042
display id: range2119042
version: 1
direction: direction/reverse
top level: pSB1C3
start: 261
end: 875
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2119045
display id: annotation2119045
version: 1
top level: pSB1C3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119045/range2119045/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119045/range2119045/1
type: Range
persistent identity: range2119045
display id: range2119045
version: 1
direction: direction/reverse
top level: pSB1C3
start: 1160
end: 1819
orientation: inline

BBa_J64100
type: ComponentDefinition
persistent identity: BBa J64100
display id: BBa_J64100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium copy ColE1 BioBrick vector
creator: Jeffrey J Tabor
created: 2007-04-02T11:00:00Z
modified: 2015-06-15T12:42:46Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _98_
m user id: 4206
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable description: This plasmid is based on pProTetE333 (clontech). A SpeI site was removed via an A-T mutation at position 1166 on the original plasmid, resulting in pProTetE333b. The G00100-G00101 fragment of pSB4A3-1 which includes P1010 between a biobricks multiple cloning site which itself is flanked by + strand transcriptional terminators was then cloned between the XhoI and AvrII sites of pProTetE333b. The resulting plasmid carries a ColE1 origin of replication, a Chloramphenicol resistance marker, the biobricks MCS and the ccdAB death cassette.
mutable notes: None
mutable provenance: pProTetE333 (clontech) and biobricks parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: prefix
sequence annotation: G00100
sequence annotation: suffix
sequence annotation: ColE1 origin
sequence annotation: P1010
sequence annotation: G00101
sequence annotation: chloramphenicol resistance marker
sequence: https://synbiohub.org/public/igem/BBa_J64100_sequence/1

prefix
type: SequenceAnnotation
persistent identity: annotation1923841
display id: annotation1923841
version: 1
top level: BBa_J64100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923841/range1923841/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923841/range1923841/1
type: Range
persistent identity: range1923841
display id: range1923841
version: 1
direction: direction/forward
top level: BBa_J64100
start: 124
end: 145
orientation: inline

G00100
type: SequenceAnnotation
persistent identity: annotation1923840
display id: annotation1923840
version: 1
top level: BBa_J64100
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923840/range1923840/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923840/range1923840/1
type: Range
persistent identity: range1923840
display id: range1923840
version: 1
direction: direction/forward
top level: BBa_J64100
start: 6
end: 25
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1923843
display id: annotation1923843
version: 1
top level: BBa_J64100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923843/range1923843/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923843/range1923843/1
type: Range
persistent identity: range1923843
display id: range1923843
version: 1
direction: direction/forward
top level: BBa_J64100
start: 821
end: 841
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1923845
display id: annotation1923845
version: 1
top level: BBa_J64100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923845/range1923845/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923845/range1923845/1
type: Range
persistent identity: range1923845
display id: range1923845
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 1130
end: 1812
orientation: inline

P1010
type: SequenceAnnotation
persistent identity: annotation1923842
display id: annotation1923842
version: 1
top level: BBa_J64100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923842/range1923842/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923842/range1923842/1
type: Range
persistent identity: range1923842
display id: range1923842
version: 1
direction: direction/forward
top level: BBa_J64100
start: 146
end: 820
orientation: inline

G00101
type: SequenceAnnotation
persistent identity: annotation1923844
display id: annotation1923844
version: 1
top level: BBa_J64100
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923844/range1923844/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923844/range1923844/1
type: Range
persistent identity: range1923844
display id: range1923844
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 977
end: 996
orientation: inline

G00101
type: SequenceAnnotation
persistent identity: annotation1931328
display id: annotation1931328
version: 1
top level: BBa_J64100
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1931328/range1931328/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1931328/range1931328/1
type: Range
persistent identity: range1931328
display id: range1931328
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 1029
end: 1048
orientation: inline

chloramphenicol resistance marker
type: SequenceAnnotation
persistent identity: annotation1923846
display id: annotation1923846
version: 1
top level: BBa_J64100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923846/range1923846/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923846/range1923846/1
type: Range
persistent identity: range1923846
display id: range1923846
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 1939
end: 2598
orientation: inline

pSB4T5
type: ComponentDefinition
persistent identity: pSB4T5
display id: pSB4T5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4T3 is a low copy plasmid with tetracycline resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is constructed from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design for more information.
mutable provenance: BBa_I51001 vector scaffold, BBa_I50040 replication origin and BBa_P1005 resistance marker.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0053
component: BBa_G0003
component: BBa_G00000
component: BBa_G00001
component: BBa_B0062
component: BBa_B0044
component: BBa_G00100
component: BBa_P1005
component: BBa_G00102
component: BBa_G0004
component: BBa_B0054
component: BBa_B0042
component: BBa_G0002
component: BBa_B0055
component: BBa_I50042
component: BBa_B0043
sequence annotation: BBa_G00001
sequence annotation: BBa_G00000
sequence annotation: BBa_B0055
sequence annotation: BBa_B0043
sequence annotation: BBa_B0062
sequence annotation: BBa_P1005
sequence annotation: BBa_I50042
sequence annotation: BBa_G0002
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_G0004
sequence annotation: BBa_G00102
sequence annotation: BBa_G00100
sequence annotation: BBa_G0003
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence: https://synbiohub.org/public/igem/pSB4T5_sequence/1

BBa_B0053
type: Component
persistent identity: component2236456
display id: component2236456
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0003
type: Component
persistent identity: component2236421
display id: component2236421
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00000
type: Component
persistent identity: component2236478
display id: component2236478
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00001
type: Component
persistent identity: component2236401
display id: component2236401
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0062
type: Component
persistent identity: component2236420
display id: component2236420
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0044
type: Component
persistent identity: component2236406
display id: component2236406
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00100
type: Component
persistent identity: component2236458
display id: component2236458
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_P1005
type: Component
persistent identity: component2236453
display id: component2236453
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1005/1

BBa_G00102
type: Component
persistent identity: component2236418
display id: component2236418
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0004
type: Component
persistent identity: component2236454
display id: component2236454
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0054
type: Component
persistent identity: component2236416
display id: component2236416
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0042
type: Component
persistent identity: component2236413
display id: component2236413
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: Component
persistent identity: component2236450
display id: component2236450
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0055
type: Component
persistent identity: component2236461
display id: component2236461
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component2236468
display id: component2236468
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_I50042
type: Component
persistent identity: component2236449
display id: component2236449
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0043
type: Component
persistent identity: component2236473
display id: component2236473
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236401
display id: annotation2236401
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236401/range2236401/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4T5/annotation2236401/range2236401/1
type: Range
persistent identity: range2236401
display id: range2236401
version: 1
direction: direction/reverse
top level: pSB4T5
start: 1
end: 21
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236478
display id: annotation2236478
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236478/range2236478/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4T5/annotation2236478/range2236478/1
type: Range
persistent identity: range2236478
display id: range2236478
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3714
end: 3735
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236461
display id: annotation2236461
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236461/range2236461/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4T5/annotation2236461/range2236461/1
type: Range
persistent identity: range2236461
display id: range2236461
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3611
end: 3688
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236473
display id: annotation2236473
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236473/range2236473/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4T5/annotation2236473/range2236473/1
type: Range
persistent identity: range2236473
display id: range2236473
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3701
end: 3713
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236420
display id: annotation2236420
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236420/range2236420/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4T5/annotation2236420/range2236420/1
type: Range
persistent identity: range2236420
display id: range2236420
version: 1
direction: direction/reverse
top level: pSB4T5
start: 136
end: 176
orientation: inline

BBa_P1005
type: SequenceAnnotation
persistent identity: annotation2236453
display id: annotation2236453
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236453/range2236453/1
component: BBa_P1005

https://synbiohub.org/public/igem/pSB4T5/annotation2236453/range2236453/1
type: Range
persistent identity: range2236453
display id: range2236453
version: 1
direction: direction/reverse
top level: pSB4T5
start: 2229
end: 3511
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236449
display id: annotation2236449
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236449/range2236449/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4T5/annotation2236449/range2236449/1
type: Range
persistent identity: range2236449
display id: range2236449
version: 1
direction: direction/reverse
top level: pSB4T5
start: 184
end: 2220
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236450
display id: annotation2236450
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236450/range2236450/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4T5/annotation2236450/range2236450/1
type: Range
persistent identity: range2236450
display id: range2236450
version: 1
direction: direction/reverse
top level: pSB4T5
start: 2221
end: 2228
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236468
display id: annotation2236468
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236468/range2236468/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4T5/annotation2236468/range2236468/1
type: Range
persistent identity: range2236468
display id: range2236468
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3689
end: 3700
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236416
display id: annotation2236416
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236416/range2236416/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4T5/annotation2236416/range2236416/1
type: Range
persistent identity: range2236416
display id: range2236416
version: 1
direction: direction/reverse
top level: pSB4T5
start: 47
end: 115
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236454
display id: annotation2236454
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236454/range2236454/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4T5/annotation2236454/range2236454/1
type: Range
persistent identity: range2236454
display id: range2236454
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3512
end: 3518
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236413
display id: annotation2236413
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236413/range2236413/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4T5/annotation2236413/range2236413/1
type: Range
persistent identity: range2236413
display id: range2236413
version: 1
direction: direction/reverse
top level: pSB4T5
start: 35
end: 46
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236418
display id: annotation2236418
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236418/range2236418/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4T5/annotation2236418/range2236418/1
type: Range
persistent identity: range2236418
display id: range2236418
version: 1
direction: direction/reverse
top level: pSB4T5
start: 116
end: 135
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236458
display id: annotation2236458
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236458/range2236458/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4T5/annotation2236458/range2236458/1
type: Range
persistent identity: range2236458
display id: range2236458
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3591
end: 3610
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236421
display id: annotation2236421
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236421/range2236421/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4T5/annotation2236421/range2236421/1
type: Range
persistent identity: range2236421
display id: range2236421
version: 1
direction: direction/reverse
top level: pSB4T5
start: 177
end: 183
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236456
display id: annotation2236456
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236456/range2236456/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4T5/annotation2236456/range2236456/1
type: Range
persistent identity: range2236456
display id: range2236456
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3519
end: 3590
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236406
display id: annotation2236406
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236406/range2236406/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4T5/annotation2236406/range2236406/1
type: Range
persistent identity: range2236406
display id: range2236406
version: 1
direction: direction/reverse
top level: pSB4T5
start: 22
end: 34
orientation: inline

pSB2K4
type: ComponentDefinition
persistent identity: pSB2K4
display id: pSB2K4
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB2K3-derived BioBrick plasmid free of many restriction sites
creator: Austin Che, Leon Chan and Sri Kosuri
created: 2006-01-19T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 22
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Derived from pSB2K3 with many restriction enzyme sites removed by site-directed mutagenesis (~2 dozen mutations). Plasmid is useful when there is a need to cut with a variety of different restriction enzymes. Most symmetric cutters have been removed, and some non-symmetric cutters have also including AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, and SgrAI. Works in exactly same way as pSB2K3. See <bb_part>pSB2K3</bb_part> for more information. Note that the VF2 site has a single base mutation in the middle of it. However, VF2 has been used successfully as a primer for PCR with this plasmid.
mutable provenance: pSB2K3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: nnpp promoter 1 (.92)'
sequence annotation: G->C
sequence annotation: lacOP
sequence annotation: repE
sequence annotation: Predicted -10
sequence annotation: Predicted transcription start
sequence annotation: BioBrick suffix
sequence annotation: G->T
sequence annotation: Possible -10
sequence annotation: repE binding site
sequence annotation: C->T
sequence annotation: nnpp promoter 3 (0.95)'
sequence annotation: T->G
sequence annotation: T->A mutation
sequence annotation: Possible -35
sequence annotation: nnpp promoter 2 (0.96)'
sequence annotation: Low GC region
sequence annotation: Stem loop
sequence annotation: Maybe A or G
sequence annotation: repL
sequence annotation: f1 ori
sequence annotation: Predicted -35
sequence annotation: E. coli his operon terminator
sequence annotation: oriS
sequence annotation: G->A
sequence annotation: T->C
sequence annotation: T->A
sequence annotation: C->A
sequence annotation: VF2 (not an exact match, but works)
sequence annotation: kanR
sequence annotation: Verification reverse
sequence annotation: A->G mutation
sequence annotation: A->C

nnpp promoter 1 (.92)'
type: SequenceAnnotation
persistent identity: annotation1974344
display id: annotation1974344
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974344/range1974344/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974344/range1974344/1
type: Range
persistent identity: range1974344
display id: range1974344
version: 1
direction: direction/forward
top level: pSB2K4
start: 3375
end: 3420
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974352
display id: annotation1974352
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974352/range1974352/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974352/range1974352/1
type: Range
persistent identity: range1974352
display id: range1974352
version: 1
direction: direction/forward
top level: pSB2K4
start: 4321
end: 4321
orientation: inline

lacOP
type: SequenceAnnotation
persistent identity: annotation1974331
display id: annotation1974331
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974331/range1974331/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974331/range1974331/1
type: Range
persistent identity: range1974331
display id: range1974331
version: 1
direction: direction/forward
top level: pSB2K4
start: 2064
end: 2238
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1974310
display id: annotation1974310
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974310/range1974310/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974310/range1974310/1
type: Range
persistent identity: range1974310
display id: range1974310
version: 1
direction: direction/reverse
top level: pSB2K4
start: 644
end: 1399
orientation: inline

Predicted -10
type: SequenceAnnotation
persistent identity: annotation1974342
display id: annotation1974342
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974342/range1974342/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974342/range1974342/1
type: Range
persistent identity: range1974342
display id: range1974342
version: 1
direction: direction/forward
top level: pSB2K4
start: 3379
end: 3387
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974314
display id: annotation1974314
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974314/range1974314/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974314/range1974314/1
type: Range
persistent identity: range1974314
display id: range1974314
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1426
end: 1426
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1974291
display id: annotation1974291
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974291/range1974291/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974291/range1974291/1
type: Range
persistent identity: range1974291
display id: range1974291
version: 1
direction: direction/forward
top level: pSB2K4
start: 1
end: 21
orientation: inline

G->T
type: SequenceAnnotation
persistent identity: annotation1974319
display id: annotation1974319
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974319/range1974319/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974319/range1974319/1
type: Range
persistent identity: range1974319
display id: range1974319
version: 1
direction: direction/forward
top level: pSB2K4
start: 1454
end: 1454
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974317
display id: annotation1974317
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974317/range1974317/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974317/range1974317/1
type: Range
persistent identity: range1974317
display id: range1974317
version: 1
direction: direction/forward
top level: pSB2K4
start: 1444
end: 1452
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974304
display id: annotation1974304
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974304/range1974304/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974304/range1974304/1
type: Range
persistent identity: range1974304
display id: range1974304
version: 1
direction: direction/reverse
top level: pSB2K4
start: 568
end: 586
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1974313
display id: annotation1974313
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974313/range1974313/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974313/range1974313/1
type: Range
persistent identity: range1974313
display id: range1974313
version: 1
direction: direction/forward
top level: pSB2K4
start: 1142
end: 1142
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974301
display id: annotation1974301
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974301/range1974301/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974301/range1974301/1
type: Range
persistent identity: range1974301
display id: range1974301
version: 1
direction: direction/reverse
top level: pSB2K4
start: 477
end: 495
orientation: inline

nnpp promoter 3 (0.95)'
type: SequenceAnnotation
persistent identity: annotation1974339
display id: annotation1974339
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974339/range1974339/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974339/range1974339/1
type: Range
persistent identity: range1974339
display id: range1974339
version: 1
direction: direction/forward
top level: pSB2K4
start: 3269
end: 3314
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974299
display id: annotation1974299
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974299/range1974299/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974299/range1974299/1
type: Range
persistent identity: range1974299
display id: range1974299
version: 1
direction: direction/reverse
top level: pSB2K4
start: 394
end: 412
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974349
display id: annotation1974349
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974349/range1974349/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974349/range1974349/1
type: Range
persistent identity: range1974349
display id: range1974349
version: 1
direction: direction/forward
top level: pSB2K4
start: 3903
end: 3903
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974326
display id: annotation1974326
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974326/range1974326/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974326/range1974326/1
type: Range
persistent identity: range1974326
display id: range1974326
version: 1
direction: direction/forward
top level: pSB2K4
start: 1552
end: 1570
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974334
display id: annotation1974334
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974334/range1974334/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974334/range1974334/1
type: Range
persistent identity: range1974334
display id: range1974334
version: 1
direction: direction/forward
top level: pSB2K4
start: 2699
end: 2699
orientation: inline

T->A mutation
type: SequenceAnnotation
persistent identity: annotation1974303
display id: annotation1974303
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974303/range1974303/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974303/range1974303/1
type: Range
persistent identity: range1974303
display id: range1974303
version: 1
direction: direction/forward
top level: pSB2K4
start: 551
end: 551
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974321
display id: annotation1974321
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974321/range1974321/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974321/range1974321/1
type: Range
persistent identity: range1974321
display id: range1974321
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1455
end: 1460
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974309
display id: annotation1974309
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974309/range1974309/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974309/range1974309/1
type: Range
persistent identity: range1974309
display id: range1974309
version: 1
direction: direction/forward
top level: pSB2K4
start: 857
end: 857
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974318
display id: annotation1974318
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974318/range1974318/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974318/range1974318/1
type: Range
persistent identity: range1974318
display id: range1974318
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1447
end: 1447
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1974350
display id: annotation1974350
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974350/range1974350/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974350/range1974350/1
type: Range
persistent identity: range1974350
display id: range1974350
version: 1
direction: direction/forward
top level: pSB2K4
start: 4119
end: 4119
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974343
display id: annotation1974343
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974343/range1974343/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974343/range1974343/1
type: Range
persistent identity: range1974343
display id: range1974343
version: 1
direction: direction/forward
top level: pSB2K4
start: 3394
end: 3394
orientation: inline

nnpp promoter 2 (0.96)'
type: SequenceAnnotation
persistent identity: annotation1974341
display id: annotation1974341
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974341/range1974341/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974341/range1974341/1
type: Range
persistent identity: range1974341
display id: range1974341
version: 1
direction: direction/forward
top level: pSB2K4
start: 3353
end: 3398
orientation: inline

Low GC region
type: SequenceAnnotation
persistent identity: annotation1974330
display id: annotation1974330
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974330/range1974330/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974330/range1974330/1
type: Range
persistent identity: range1974330
display id: range1974330
version: 1
direction: direction/forward
top level: pSB2K4
start: 1823
end: 1844
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974327
display id: annotation1974327
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974327/range1974327/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974327/range1974327/1
type: Range
persistent identity: range1974327
display id: range1974327
version: 1
direction: direction/forward
top level: pSB2K4
start: 1581
end: 1591
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1974293
display id: annotation1974293
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974293/range1974293/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974293/range1974293/1
type: Range
persistent identity: range1974293
display id: range1974293
version: 1
direction: direction/forward
top level: pSB2K4
start: 59
end: 91
orientation: inline

Maybe A or G
type: SequenceAnnotation
persistent identity: annotation1974332
display id: annotation1974332
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974332/range1974332/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974332/range1974332/1
type: Range
persistent identity: range1974332
display id: range1974332
version: 1
direction: direction/forward
top level: pSB2K4
start: 2565
end: 2565
orientation: inline

repL
type: SequenceAnnotation
persistent identity: annotation1974335
display id: annotation1974335
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974335/range1974335/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974335/range1974335/1
type: Range
persistent identity: range1974335
display id: range1974335
version: 1
direction: direction/forward
top level: pSB2K4
start: 2316
end: 3161
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1974354
display id: annotation1974354
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974354/range1974354/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974354/range1974354/1
type: Range
persistent identity: range1974354
display id: range1974354
version: 1
direction: direction/forward
top level: pSB2K4
start: 4402
end: 4421
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation1974297
display id: annotation1974297
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974297/range1974297/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974297/range1974297/1
type: Range
persistent identity: range1974297
display id: range1974297
version: 1
direction: direction/reverse
top level: pSB2K4
start: 269
end: 317
orientation: inline

Predicted -35
type: SequenceAnnotation
persistent identity: annotation1974340
display id: annotation1974340
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974340/range1974340/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974340/range1974340/1
type: Range
persistent identity: range1974340
display id: range1974340
version: 1
direction: direction/forward
top level: pSB2K4
start: 3359
end: 3364
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974316
display id: annotation1974316
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974316/range1974316/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974316/range1974316/1
type: Range
persistent identity: range1974316
display id: range1974316
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1433
end: 1441
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1974292
display id: annotation1974292
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974292/range1974292/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974292/range1974292/1
type: Range
persistent identity: range1974292
display id: range1974292
version: 1
direction: direction/forward
top level: pSB2K4
start: 51
end: 120
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974315
display id: annotation1974315
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974315/range1974315/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974315/range1974315/1
type: Range
persistent identity: range1974315
display id: range1974315
version: 1
direction: direction/forward
top level: pSB2K4
start: 1426
end: 1431
orientation: inline

oriS
type: SequenceAnnotation
persistent identity: annotation1974328
display id: annotation1974328
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974328/range1974328/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974328/range1974328/1
type: Range
persistent identity: range1974328
display id: range1974328
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1490
end: 1706
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation1974337
display id: annotation1974337
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974337/range1974337/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974337/range1974337/1
type: Range
persistent identity: range1974337
display id: range1974337
version: 1
direction: direction/forward
top level: pSB2K4
start: 3017
end: 3017
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974305
display id: annotation1974305
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974305/range1974305/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974305/range1974305/1
type: Range
persistent identity: range1974305
display id: range1974305
version: 1
direction: direction/forward
top level: pSB2K4
start: 578
end: 578
orientation: inline

T->A
type: SequenceAnnotation
persistent identity: annotation1974345
display id: annotation1974345
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974345/range1974345/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974345/range1974345/1
type: Range
persistent identity: range1974345
display id: range1974345
version: 1
direction: direction/forward
top level: pSB2K4
start: 3507
end: 3507
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974338
display id: annotation1974338
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974338/range1974338/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974338/range1974338/1
type: Range
persistent identity: range1974338
display id: range1974338
version: 1
direction: direction/forward
top level: pSB2K4
start: 3251
end: 3251
orientation: inline

C->A
type: SequenceAnnotation
persistent identity: annotation1974295
display id: annotation1974295
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974295/range1974295/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974295/range1974295/1
type: Range
persistent identity: range1974295
display id: range1974295
version: 1
direction: direction/forward
top level: pSB2K4
start: 146
end: 146
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974322
display id: annotation1974322
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974322/range1974322/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974322/range1974322/1
type: Range
persistent identity: range1974322
display id: range1974322
version: 1
direction: direction/forward
top level: pSB2K4
start: 1460
end: 1460
orientation: inline

VF2 (not an exact match, but works)
type: SequenceAnnotation
persistent identity: annotation1974351
display id: annotation1974351
version: 1
top level: pSB2K4
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974351/range1974351/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974351/range1974351/1
type: Range
persistent identity: range1974351
display id: range1974351
version: 1
direction: direction/forward
top level: pSB2K4
start: 4313
end: 4332
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974312
display id: annotation1974312
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974312/range1974312/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974312/range1974312/1
type: Range
persistent identity: range1974312
display id: range1974312
version: 1
direction: direction/forward
top level: pSB2K4
start: 1100
end: 1100
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1974348
display id: annotation1974348
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974348/range1974348/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974348/range1974348/1
type: Range
persistent identity: range1974348
display id: range1974348
version: 1
direction: direction/forward
top level: pSB2K4
start: 3460
end: 4275
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974294
display id: annotation1974294
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974294/range1974294/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974294/range1974294/1
type: Range
persistent identity: range1974294
display id: range1974294
version: 1
direction: direction/forward
top level: pSB2K4
start: 93
end: 93
orientation: inline

Verification reverse
type: SequenceAnnotation
persistent identity: annotation1974296
display id: annotation1974296
version: 1
top level: pSB2K4
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974296/range1974296/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974296/range1974296/1
type: Range
persistent identity: range1974296
display id: range1974296
version: 1
direction: direction/reverse
top level: pSB2K4
start: 184
end: 203
orientation: inline

A->G mutation
type: SequenceAnnotation
persistent identity: annotation1974300
display id: annotation1974300
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974300/range1974300/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974300/range1974300/1
type: Range
persistent identity: range1974300
display id: range1974300
version: 1
direction: direction/forward
top level: pSB2K4
start: 462
end: 462
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974306
display id: annotation1974306
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974306/range1974306/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974306/range1974306/1
type: Range
persistent identity: range1974306
display id: range1974306
version: 1
direction: direction/reverse
top level: pSB2K4
start: 597
end: 619
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974333
display id: annotation1974333
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974333/range1974333/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974333/range1974333/1
type: Range
persistent identity: range1974333
display id: range1974333
version: 1
direction: direction/forward
top level: pSB2K4
start: 2567
end: 2567
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974320
display id: annotation1974320
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974320/range1974320/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974320/range1974320/1
type: Range
persistent identity: range1974320
display id: range1974320
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1455
end: 1463
orientation: inline

A->C
type: SequenceAnnotation
persistent identity: annotation1974308
display id: annotation1974308
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974308/range1974308/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974308/range1974308/1
type: Range
persistent identity: range1974308
display id: range1974308
version: 1
direction: direction/forward
top level: pSB2K4
start: 692
end: 692
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974323
display id: annotation1974323
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974323/range1974323/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974323/range1974323/1
type: Range
persistent identity: range1974323
display id: range1974323
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1477
end: 1482
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1974355
display id: annotation1974355
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974355/range1974355/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974355/range1974355/1
type: Range
persistent identity: range1974355
display id: range1974355
version: 1
direction: direction/forward
top level: pSB2K4
start: 4446
end: 4467
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974307
display id: annotation1974307
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974307/range1974307/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974307/range1974307/1
type: Range
persistent identity: range1974307
display id: range1974307
version: 1
direction: direction/reverse
top level: pSB2K4
start: 623
end: 641
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1974353
display id: annotation1974353
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974353/range1974353/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974353/range1974353/1
type: Range
persistent identity: range1974353
display id: range1974353
version: 1
direction: direction/forward
top level: pSB2K4
start: 4389
end: 4392
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974336
display id: annotation1974336
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974336/range1974336/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974336/range1974336/1
type: Range
persistent identity: range1974336
display id: range1974336
version: 1
direction: direction/forward
top level: pSB2K4
start: 2774
end: 2774
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974346
display id: annotation1974346
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974346/range1974346/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974346/range1974346/1
type: Range
persistent identity: range1974346
display id: range1974346
version: 1
direction: direction/forward
top level: pSB2K4
start: 3702
end: 3702
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974311
display id: annotation1974311
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974311/range1974311/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974311/range1974311/1
type: Range
persistent identity: range1974311
display id: range1974311
version: 1
direction: direction/forward
top level: pSB2K4
start: 1091
end: 1091
orientation: inline

Low GC region
type: SequenceAnnotation
persistent identity: annotation1974329
display id: annotation1974329
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974329/range1974329/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974329/range1974329/1
type: Range
persistent identity: range1974329
display id: range1974329
version: 1
direction: direction/forward
top level: pSB2K4
start: 1636
end: 1682
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974347
display id: annotation1974347
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974347/range1974347/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974347/range1974347/1
type: Range
persistent identity: range1974347
display id: range1974347
version: 1
direction: direction/forward
top level: pSB2K4
start: 3849
end: 3849
orientation: inline

T->A
type: SequenceAnnotation
persistent identity: annotation1974298
display id: annotation1974298
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974298/range1974298/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974298/range1974298/1
type: Range
persistent identity: range1974298
display id: range1974298
version: 1
direction: direction/forward
top level: pSB2K4
start: 292
end: 292
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974325
display id: annotation1974325
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974325/range1974325/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974325/range1974325/1
type: Range
persistent identity: range1974325
display id: range1974325
version: 1
direction: direction/forward
top level: pSB2K4
start: 1527
end: 1537
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974324
display id: annotation1974324
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974324/range1974324/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974324/range1974324/1
type: Range
persistent identity: range1974324
display id: range1974324
version: 1
direction: direction/forward
top level: pSB2K4
start: 1497
end: 1515
orientation: inline

incC
type: SequenceAnnotation
persistent identity: annotation1974302
display id: annotation1974302
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974302/range1974302/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974302/range1974302/1
type: Range
persistent identity: range1974302
display id: range1974302
version: 1
direction: direction/reverse
top level: pSB2K4
start: 394
end: 641
orientation: inline

pSB4K50
type: ComponentDefinition
persistent identity: BBa K1362097
display id: BBa_K1362097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick expression backbone carrying Kan resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0044
component: BBa_I50041
component: BBa_B0043
component: BBa_K1362463
component: BBa_B0042
component: BBa_B0054
component: BBa_G00102
component: BBa_G00000
component: BBa_B0055
component: BBa_P1007
component: BBa_B0053
component: BBa_B0062
component: BBa_G0005
component: BBa_G0001
component: BBa_G00100
component: BBa_G0006
component: BBa_G00001
sequence annotation: BBa_B0055
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_B0062
sequence annotation: BBa_G00001
sequence annotation: BBa_G00102
sequence annotation: BBa_I50041
sequence annotation: BBa_G00100
sequence annotation: BBa_G0006
sequence annotation: BBa_G0005
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_B0043
sequence annotation: BBa_K1362463
sequence annotation: BBa_G0001
sequence annotation: BBa_P1007
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_K1362097_sequence/1

BBa_B0044
type: Component
persistent identity: component2409942
display id: component2409942
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_I50041
type: Component
persistent identity: component2409988
display id: component2409988
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50041/1

BBa_B0043
type: Component
persistent identity: component2410008
display id: component2410008
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_K1362463
type: Component
persistent identity: component2410012
display id: component2410012
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_B0042
type: Component
persistent identity: component2410003
display id: component2410003
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0054
type: Component
persistent identity: component2409952
display id: component2409952
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0042
type: Component
persistent identity: component2409949
display id: component2409949
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00102
type: Component
persistent identity: component2409954
display id: component2409954
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G00000
type: Component
persistent identity: component2410017
display id: component2410017
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0055
type: Component
persistent identity: component2409996
display id: component2409996
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_P1007
type: Component
persistent identity: component2409961
display id: component2409961
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1007/1

BBa_B0053
type: Component
persistent identity: component2409991
display id: component2409991
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0062
type: Component
persistent identity: component2409956
display id: component2409956
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0005
type: Component
persistent identity: component2409957
display id: component2409957
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0005/1

BBa_G0001
type: Component
persistent identity: component2409962
display id: component2409962
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0001/1

BBa_G00100
type: Component
persistent identity: component2409993
display id: component2409993
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G0006
type: Component
persistent identity: component2409989
display id: component2409989
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0006/1

BBa_G00001
type: Component
persistent identity: component2409937
display id: component2409937
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409996
display id: annotation2409996
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409996/range2409996/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409996/range2409996/1
type: Range
persistent identity: range2409996
display id: range2409996
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3295
end: 3372
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2410003
display id: annotation2410003
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410003/range2410003/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410003/range2410003/1
type: Range
persistent identity: range2410003
display id: range2410003
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3373
end: 3384
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409952
display id: annotation2409952
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409952/range2409952/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409952/range2409952/1
type: Range
persistent identity: range2409952
display id: range2409952
version: 1
direction: direction/reverse
top level: pSB4K50
start: 47
end: 115
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409956
display id: annotation2409956
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409956/range2409956/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409956/range2409956/1
type: Range
persistent identity: range2409956
display id: range2409956
version: 1
direction: direction/reverse
top level: pSB4K50
start: 136
end: 176
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409937
display id: annotation2409937
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409937/range2409937/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409937/range2409937/1
type: Range
persistent identity: range2409937
display id: range2409937
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1
end: 21
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409954
display id: annotation2409954
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409954/range2409954/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409954/range2409954/1
type: Range
persistent identity: range2409954
display id: range2409954
version: 1
direction: direction/reverse
top level: pSB4K50
start: 116
end: 135
orientation: inline

BBa_I50041
type: SequenceAnnotation
persistent identity: annotation2409988
display id: annotation2409988
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409988/range2409988/1
component: BBa_I50041

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409988/range2409988/1
type: Range
persistent identity: range2409988
display id: range2409988
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1159
end: 3195
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409993
display id: annotation2409993
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409993/range2409993/1
component: BBa_G00100

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type: Range
persistent identity: range2409993
display id: range2409993
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3275
end: 3294
orientation: inline

BBa_G0006
type: SequenceAnnotation
persistent identity: annotation2409989
display id: annotation2409989
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409989/range2409989/1
component: BBa_G0006

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409989/range2409989/1
type: Range
persistent identity: range2409989
display id: range2409989
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3196
end: 3202
orientation: inline

BBa_G0005
type: SequenceAnnotation
persistent identity: annotation2409957
display id: annotation2409957
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409957/range2409957/1
component: BBa_G0005

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409957/range2409957/1
type: Range
persistent identity: range2409957
display id: range2409957
version: 1
direction: direction/reverse
top level: pSB4K50
start: 177
end: 183
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409991
display id: annotation2409991
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409991/range2409991/1
component: BBa_B0053

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type: Range
persistent identity: range2409991
display id: range2409991
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3203
end: 3274
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409942
display id: annotation2409942
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409942/range2409942/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409942/range2409942/1
type: Range
persistent identity: range2409942
display id: range2409942
version: 1
direction: direction/reverse
top level: pSB4K50
start: 22
end: 34
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2410008
display id: annotation2410008
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410008/range2410008/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410008/range2410008/1
type: Range
persistent identity: range2410008
display id: range2410008
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3385
end: 3397
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2410012
display id: annotation2410012
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
type: Range
persistent identity: range2410012
display id: range2410012
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3398
end: 3448
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409949
display id: annotation2409949
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409949/range2409949/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409949/range2409949/1
type: Range
persistent identity: range2409949
display id: range2409949
version: 1
direction: direction/reverse
top level: pSB4K50
start: 35
end: 46
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2409962
display id: annotation2409962
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409962/range2409962/1
component: BBa_G0001

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409962/range2409962/1
type: Range
persistent identity: range2409962
display id: range2409962
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1151
end: 1158
orientation: inline

BBa_P1007
type: SequenceAnnotation
persistent identity: annotation2409961
display id: annotation2409961
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409961/range2409961/1
component: BBa_P1007

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type: Range
persistent identity: range2409961
display id: range2409961
version: 1
direction: direction/reverse
top level: pSB4K50
start: 184
end: 1150
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2410017
display id: annotation2410017
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410017/range2410017/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410017/range2410017/1
type: Range
persistent identity: range2410017
display id: range2410017
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3449
end: 3470
orientation: inline

pSB4C5
type: ComponentDefinition
persistent identity: pSB4C5
display id: pSB4C5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-26T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4C3 is a low copy plasmid based with chloramphenicol resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) pSB4C5 is derived from the BioBricks vector scaffold BBa_I51001. However, the direction of the resistance marker and replication origin is not optimal.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, pSC101 based origin and a version of the chloramphenicol rsistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00001
component: BBa_G00000
component: BBa_B0044
component: BBa_I50042
component: BBa_B0043
component: BBa_P1004
component: BBa_B0062
component: BBa_B0053
component: BBa_B0054
component: BBa_G00102
component: BBa_B0042
component: BBa_B0055
component: BBa_G00100
component: BBa_G0003
component: BBa_G0004
component: BBa_G0002
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_B0044
sequence annotation: BBa_B0062
sequence annotation: BBa_G0002
sequence annotation: BBa_B0055
sequence annotation: BBa_G00000
sequence annotation: BBa_G00100
sequence annotation: BBa_G00001
sequence annotation: BBa_P1004
sequence annotation: BBa_I50042
sequence annotation: BBa_G0004
sequence annotation: BBa_B0043
sequence annotation: BBa_G0003
sequence annotation: BBa_B0053
sequence annotation: BBa_G00102
sequence: https://synbiohub.org/public/igem/pSB4C5_sequence/1

BBa_G00001
type: Component
persistent identity: component2236236
display id: component2236236
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00000
type: Component
persistent identity: component2236312
display id: component2236312
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0044
type: Component
persistent identity: component2236241
display id: component2236241
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_I50042
type: Component
persistent identity: component2236284
display id: component2236284
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0043
type: Component
persistent identity: component2236307
display id: component2236307
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_P1004
type: Component
persistent identity: component2236287
display id: component2236287
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0062
type: Component
persistent identity: component2236255
display id: component2236255
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0053
type: Component
persistent identity: component2236290
display id: component2236290
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0054
type: Component
persistent identity: component2236251
display id: component2236251
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00102
type: Component
persistent identity: component2236253
display id: component2236253
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component2236248
display id: component2236248
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0055
type: Component
persistent identity: component2236295
display id: component2236295
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00100
type: Component
persistent identity: component2236292
display id: component2236292
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0042
type: Component
persistent identity: component2236302
display id: component2236302
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0003
type: Component
persistent identity: component2236256
display id: component2236256
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G0004
type: Component
persistent identity: component2236288
display id: component2236288
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G0002
type: Component
persistent identity: component2236285
display id: component2236285
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236302
display id: annotation2236302
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236302/range2236302/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4C5/annotation2236302/range2236302/1
type: Range
persistent identity: range2236302
display id: range2236302
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3175
end: 3186
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236251
display id: annotation2236251
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236251/range2236251/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4C5/annotation2236251/range2236251/1
type: Range
persistent identity: range2236251
display id: range2236251
version: 1
direction: direction/reverse
top level: pSB4C5
start: 47
end: 115
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236241
display id: annotation2236241
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236241/range2236241/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4C5/annotation2236241/range2236241/1
type: Range
persistent identity: range2236241
display id: range2236241
version: 1
direction: direction/reverse
top level: pSB4C5
start: 22
end: 34
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236255
display id: annotation2236255
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236255/range2236255/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4C5/annotation2236255/range2236255/1
type: Range
persistent identity: range2236255
display id: range2236255
version: 1
direction: direction/reverse
top level: pSB4C5
start: 136
end: 176
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236285
display id: annotation2236285
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236285/range2236285/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4C5/annotation2236285/range2236285/1
type: Range
persistent identity: range2236285
display id: range2236285
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2221
end: 2228
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236295
display id: annotation2236295
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236295/range2236295/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4C5/annotation2236295/range2236295/1
type: Range
persistent identity: range2236295
display id: range2236295
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3097
end: 3174
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236312
display id: annotation2236312
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236312/range2236312/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4C5/annotation2236312/range2236312/1
type: Range
persistent identity: range2236312
display id: range2236312
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3200
end: 3221
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236292
display id: annotation2236292
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236292/range2236292/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4C5/annotation2236292/range2236292/1
type: Range
persistent identity: range2236292
display id: range2236292
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3077
end: 3096
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236236
display id: annotation2236236
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236236/range2236236/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4C5/annotation2236236/range2236236/1
type: Range
persistent identity: range2236236
display id: range2236236
version: 1
direction: direction/reverse
top level: pSB4C5
start: 1
end: 21
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2236287
display id: annotation2236287
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236287/range2236287/1
component: BBa_P1004

https://synbiohub.org/public/igem/pSB4C5/annotation2236287/range2236287/1
type: Range
persistent identity: range2236287
display id: range2236287
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2229
end: 2997
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236284
display id: annotation2236284
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236284/range2236284/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4C5/annotation2236284/range2236284/1
type: Range
persistent identity: range2236284
display id: range2236284
version: 1
direction: direction/reverse
top level: pSB4C5
start: 184
end: 2220
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236288
display id: annotation2236288
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236288/range2236288/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4C5/annotation2236288/range2236288/1
type: Range
persistent identity: range2236288
display id: range2236288
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2998
end: 3004
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236307
display id: annotation2236307
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236307/range2236307/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4C5/annotation2236307/range2236307/1
type: Range
persistent identity: range2236307
display id: range2236307
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3187
end: 3199
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236256
display id: annotation2236256
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236256/range2236256/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4C5/annotation2236256/range2236256/1
type: Range
persistent identity: range2236256
display id: range2236256
version: 1
direction: direction/reverse
top level: pSB4C5
start: 177
end: 183
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236290
display id: annotation2236290
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236290/range2236290/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4C5/annotation2236290/range2236290/1
type: Range
persistent identity: range2236290
display id: range2236290
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3005
end: 3076
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236248
display id: annotation2236248
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236248/range2236248/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4C5/annotation2236248/range2236248/1
type: Range
persistent identity: range2236248
display id: range2236248
version: 1
direction: direction/reverse
top level: pSB4C5
start: 35
end: 46
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236253
display id: annotation2236253
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236253/range2236253/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4C5/annotation2236253/range2236253/1
type: Range
persistent identity: range2236253
display id: range2236253
version: 1
direction: direction/reverse
top level: pSB4C5
start: 116
end: 135
orientation: inline

BBa_K864011
type: ComponentDefinition
persistent identity: BBa K864011
display id: BBa_K864011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864011_sequence/1

BBa_K864009
type: ComponentDefinition
persistent identity: BBa K864009
display id: BBa_K864009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: -35
sequence annotation: rrnC
sequence annotation: pSC101 ori
sequence annotation: lacI
sequence annotation: Promoter
sequence annotation: Lux terminator
sequence annotation: -10
sequence annotation: cmR
sequence annotation: PlacIq promoter
sequence annotation: tonB terminator
sequence annotation: T3 terminator
sequence annotation: RBS
sequence annotation: P22 late terminator
sequence: https://synbiohub.org/public/igem/BBa_K864009_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2202850
display id: annotation2202850
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202850/range2202850/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202850/range2202850/1
type: Range
persistent identity: range2202850
display id: range2202850
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2441
end: 2446
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation2202855
display id: annotation2202855
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202855/range2202855/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202855/range2202855/1
type: Range
persistent identity: range2202855
display id: range2202855
version: 1
direction: direction/forward
top level: BBa_K864009
start: 136
end: 175
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2202828
display id: annotation2202828
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202828/range2202828/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202828/range2202828/1
type: Range
persistent identity: range2202828
display id: range2202828
version: 1
direction: direction/forward
top level: BBa_K864009
start: 190
end: 2405
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2202846
display id: annotation2202846
version: 1
top level: BBa_K864009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202846/range2202846/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202846/range2202846/1
type: Range
persistent identity: range2202846
display id: range2202846
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2521
end: 3603
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2202844
display id: annotation2202844
version: 1
top level: BBa_K864009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202844/range2202844/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202844/range2202844/1
type: Range
persistent identity: range2202844
display id: range2202844
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 4415
end: 4441
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2202851
display id: annotation2202851
version: 1
top level: BBa_K864009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202851/range2202851/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202851/range2202851/1
type: Range
persistent identity: range2202851
display id: range2202851
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 3634
end: 3679
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2202857
display id: annotation2202857
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202857/range2202857/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202857/range2202857/1
type: Range
persistent identity: range2202857
display id: range2202857
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2464
end: 2469
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2202856
display id: annotation2202856
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202856/range2202856/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202856/range2202856/1
type: Range
persistent identity: range2202856
display id: range2202856
version: 1
direction: direction/forward
top level: BBa_K864009
start: 903
end: 908
orientation: inline

cmR
type: SequenceAnnotation
persistent identity: annotation2202847
display id: annotation2202847
version: 1
top level: BBa_K864009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202847/range2202847/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202847/range2202847/1
type: Range
persistent identity: range2202847
display id: range2202847
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 3686
end: 4348
orientation: inline

PlacIq promoter
type: SequenceAnnotation
persistent identity: annotation2202845
display id: annotation2202845
version: 1
top level: BBa_K864009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202845/range2202845/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202845/range2202845/1
type: Range
persistent identity: range2202845
display id: range2202845
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2439
end: 2494
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2202854
display id: annotation2202854
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202854/range2202854/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202854/range2202854/1
type: Range
persistent identity: range2202854
display id: range2202854
version: 1
direction: direction/forward
top level: BBa_K864009
start: 47
end: 115
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2202853
display id: annotation2202853
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202853/range2202853/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202853/range2202853/1
type: Range
persistent identity: range2202853
display id: range2202853
version: 1
direction: direction/forward
top level: BBa_K864009
start: 4522
end: 4599
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2202848
display id: annotation2202848
version: 1
top level: BBa_K864009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202848/range2202848/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202848/range2202848/1
type: Range
persistent identity: range2202848
display id: range2202848
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2509
end: 2514
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2202852
display id: annotation2202852
version: 1
top level: BBa_K864009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202852/range2202852/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202852/range2202852/1
type: Range
persistent identity: range2202852
display id: range2202852
version: 1
direction: direction/forward
top level: BBa_K864009
start: 4461
end: 4501
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2202849
display id: annotation2202849
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202849/range2202849/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202849/range2202849/1
type: Range
persistent identity: range2202849
display id: range2202849
version: 1
direction: direction/forward
top level: BBa_K864009
start: 881
end: 886
orientation: inline

BBa_K864007
type: ComponentDefinition
persistent identity: BBa K864007
display id: BBa_K864007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4S15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864007_sequence/1

pSB8S15
type: ComponentDefinition
persistent identity: BBa K864019
display id: BBa_K864019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8S15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864019_sequence/1

pSB3K1
type: ComponentDefinition
persistent identity: pSB3K1
display id: pSB3K1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pACYC177 converted into BioBrick vector (formerly BBa_I739202)
creator: Stefan Luzi
created: 2007-10-22T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: p15A replication origin
sequence annotation: Tn903 inverted repeat
sequence annotation: partial Tn903 repressor fragment
sequence annotation: BioBrick prefix
sequence annotation: partial ampicillin resistance marker (nonfunctional)
sequence annotation: kanamycin resistance marker
sequence annotation: Tn3 inverted repeat
sequence annotation: BioBrick suffix
sequence: https://synbiohub.org/public/igem/pSB3K1_sequence/1

p15A replication origin
type: SequenceAnnotation
persistent identity: annotation1969622
display id: annotation1969622
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969622/range1969622/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969622/range1969622/1
type: Range
persistent identity: range1969622
display id: range1969622
version: 1
direction: direction/forward
top level: pSB3K1
start: 483
end: 1312
orientation: inline

Tn903 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969626
display id: annotation1969626
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969626/range1969626/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969626/range1969626/1
type: Range
persistent identity: range1969626
display id: range1969626
version: 1
direction: direction/forward
top level: pSB3K1
start: 1307
end: 1534
orientation: inline

partial Tn903 repressor fragment
type: SequenceAnnotation
persistent identity: annotation1969628
display id: annotation1969628
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969628/range1969628/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969628/range1969628/1
type: Range
persistent identity: range1969628
display id: range1969628
version: 1
direction: direction/forward
top level: pSB3K1
start: 2742
end: 3036
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969630
display id: annotation1969630
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969630/range1969630/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969630/range1969630/1
type: Range
persistent identity: range1969630
display id: range1969630
version: 1
direction: direction/forward
top level: pSB3K1
start: 3043
end: 3064
orientation: inline

partial ampicillin resistance marker (nonfunctional)
type: SequenceAnnotation
persistent identity: annotation1969624
display id: annotation1969624
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969624/range1969624/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969624/range1969624/1
type: Range
persistent identity: range1969624
display id: range1969624
version: 1
direction: direction/forward
top level: pSB3K1
start: 22
end: 332
orientation: inline

Tn903 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969627
display id: annotation1969627
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969627/range1969627/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969627/range1969627/1
type: Range
persistent identity: range1969627
display id: range1969627
version: 1
direction: direction/forward
top level: pSB3K1
start: 2515
end: 2742
orientation: inline

kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation1969623
display id: annotation1969623
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969623/range1969623/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969623/range1969623/1
type: Range
persistent identity: range1969623
display id: range1969623
version: 1
direction: direction/forward
top level: pSB3K1
start: 1637
end: 2453
orientation: inline

Tn3 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969625
display id: annotation1969625
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969625/range1969625/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969625/range1969625/1
type: Range
persistent identity: range1969625
display id: range1969625
version: 1
direction: direction/forward
top level: pSB3K1
start: 445
end: 482
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969629
display id: annotation1969629
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969629/range1969629/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969629/range1969629/1
type: Range
persistent identity: range1969629
display id: range1969629
version: 1
direction: direction/forward
top level: pSB3K1
start: 1
end: 21
orientation: inline

pSB1T3
type: ComponentDefinition
persistent identity: pSB1T3
display id: pSB1T3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Austin Che
created: 2008-09-07T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.
mutable notes: The ampicillin resistance marker needed to be eliminated.
mutable provenance: Austin Che constructed this plamid from pSB1AT3-P1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: C->T mutation
sequence annotation: Bind Site 2
sequence annotation: E. coli his operon terminator
sequence annotation: BioBrick suffix
sequence annotation: Bind Site 5
sequence annotation: two base pair AC deletion
sequence annotation: Promoter P1
sequence annotation: ORI
sequence annotation: rep (pMB1)
sequence annotation: Stem loop
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: Tetracycline resistance
sequence annotation: Verifiction forward (VF2) primer binding site
sequence annotation: Bind Site 3
sequence annotation: Bind Site 1
sequence annotation: Bind Site 4
sequence annotation: BioBrick prefix
sequence annotation: Promoter P2
sequence annotation: end of terminator
sequence: https://synbiohub.org/public/igem/pSB1T3_sequence/1

C->T mutation
type: SequenceAnnotation
persistent identity: annotation1975551
display id: annotation1975551
version: 1
top level: pSB1T3
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975551/range1975551/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975551/range1975551/1
type: Range
persistent identity: range1975551
display id: range1975551
version: 1
direction: direction/forward
top level: pSB1T3
start: 2175
end: 2175
orientation: inline

Bind Site 2
type: SequenceAnnotation
persistent identity: annotation1975545
display id: annotation1975545
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975545/range1975545/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975545/range1975545/1
type: Range
persistent identity: range1975545
display id: range1975545
version: 1
direction: direction/forward
top level: pSB1T3
start: 1080
end: 1083
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1975539
display id: annotation1975539
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975539/range1975539/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975539/range1975539/1
type: Range
persistent identity: range1975539
display id: range1975539
version: 1
direction: direction/forward
top level: pSB1T3
start: 22
end: 93
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975537
display id: annotation1975537
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975537/range1975537/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975537/range1975537/1
type: Range
persistent identity: range1975537
display id: range1975537
version: 1
direction: direction/forward
top level: pSB1T3
start: 1
end: 21
orientation: inline

Bind Site 5
type: SequenceAnnotation
persistent identity: annotation1975549
display id: annotation1975549
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975549/range1975549/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975549/range1975549/1
type: Range
persistent identity: range1975549
display id: range1975549
version: 1
direction: direction/forward
top level: pSB1T3
start: 1121
end: 1124
orientation: inline

two base pair AC deletion
type: SequenceAnnotation
persistent identity: annotation1975536
display id: annotation1975536
version: 1
top level: pSB1T3
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975536/range1975536/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975536/range1975536/1
type: Range
persistent identity: range1975536
display id: range1975536
version: 1
direction: direction/forward
top level: pSB1T3
start: 34
end: 35
orientation: inline

Promoter P1
type: SequenceAnnotation
persistent identity: annotation1975543
display id: annotation1975543
version: 1
top level: pSB1T3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975543/range1975543/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975543/range1975543/1
type: Range
persistent identity: range1975543
display id: range1975543
version: 1
direction: direction/reverse
top level: pSB1T3
start: 1068
end: 1074
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1975541
display id: annotation1975541
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975541/range1975541/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975541/range1975541/1
type: Range
persistent identity: range1975541
display id: range1975541
version: 1
direction: direction/forward
top level: pSB1T3
start: 276
end: 276
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1975542
display id: annotation1975542
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975542/range1975542/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975542/range1975542/1
type: Range
persistent identity: range1975542
display id: range1975542
version: 1
direction: direction/reverse
top level: pSB1T3
start: 261
end: 875
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975554
display id: annotation1975554
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975554/range1975554/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975554/range1975554/1
type: Range
persistent identity: range1975554
display id: range1975554
version: 1
direction: direction/reverse
top level: pSB1T3
start: 2413
end: 2432
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975540
display id: annotation1975540
version: 1
top level: pSB1T3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975540/range1975540/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975540/range1975540/1
type: Range
persistent identity: range1975540
display id: range1975540
version: 1
direction: direction/reverse
top level: pSB1T3
start: 157
end: 176
orientation: inline

Tetracycline resistance
type: SequenceAnnotation
persistent identity: annotation1975550
display id: annotation1975550
version: 1
top level: pSB1T3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975550/range1975550/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975550/range1975550/1
type: Range
persistent identity: range1975550
display id: range1975550
version: 1
direction: direction/forward
top level: pSB1T3
start: 1127
end: 2317
orientation: inline

Verifiction forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975552
display id: annotation1975552
version: 1
top level: pSB1T3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975552/range1975552/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975552/range1975552/1
type: Range
persistent identity: range1975552
display id: range1975552
version: 1
direction: direction/forward
top level: pSB1T3
start: 2324
end: 2343
orientation: inline

Bind Site 3
type: SequenceAnnotation
persistent identity: annotation1975546
display id: annotation1975546
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975546/range1975546/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975546/range1975546/1
type: Range
persistent identity: range1975546
display id: range1975546
version: 1
direction: direction/forward
top level: pSB1T3
start: 1094
end: 1097
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975538
display id: annotation1975538
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975538/range1975538/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975538/range1975538/1
type: Range
persistent identity: range1975538
display id: range1975538
version: 1
direction: direction/forward
top level: pSB1T3
start: 30
end: 64
orientation: inline

Bind Site 1
type: SequenceAnnotation
persistent identity: annotation1975544
display id: annotation1975544
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975544/range1975544/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975544/range1975544/1
type: Range
persistent identity: range1975544
display id: range1975544
version: 1
direction: direction/forward
top level: pSB1T3
start: 1065
end: 1068
orientation: inline

Bind Site 4
type: SequenceAnnotation
persistent identity: annotation1975548
display id: annotation1975548
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975548/range1975548/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975548/range1975548/1
type: Range
persistent identity: range1975548
display id: range1975548
version: 1
direction: direction/forward
top level: pSB1T3
start: 1108
end: 1111
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1975555
display id: annotation1975555
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975555/range1975555/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975555/range1975555/1
type: Range
persistent identity: range1975555
display id: range1975555
version: 1
direction: direction/forward
top level: pSB1T3
start: 2442
end: 2463
orientation: inline

Promoter P2
type: SequenceAnnotation
persistent identity: annotation1975547
display id: annotation1975547
version: 1
top level: pSB1T3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975547/range1975547/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975547/range1975547/1
type: Range
persistent identity: range1975547
display id: range1975547
version: 1
direction: direction/forward
top level: pSB1T3
start: 1084
end: 1090
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1975553
display id: annotation1975553
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975553/range1975553/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975553/range1975553/1
type: Range
persistent identity: range1975553
display id: range1975553
version: 1
direction: direction/reverse
top level: pSB1T3
start: 2400
end: 2403
orientation: inline

BBa_K864010
type: ComponentDefinition
persistent identity: BBa K864010
display id: BBa_K864010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864010_sequence/1

pSBBs1C
type: ComponentDefinition
persistent identity: BBa K823023
display id: BBa_K823023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs1C: Empty backbone for integration into Bacillus subtilis amyE locus
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the amyE locus and can be selected with Chloramphenicol (cat gene). It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1662. This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR.
mutable provenance: This backbone is an igem compatible version of the Bacillus vector pDG1662
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: amyE
sequence annotation: bla
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K823023_sequence/1

amyE
type: SequenceAnnotation
persistent identity: annotation2180239
display id: annotation2180239
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180239/range2180239/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180239/range2180239/1
type: Range
persistent identity: range2180239
display id: range2180239
version: 1
direction: direction/forward
top level: pSBBs1C
start: 5546
end: 6066
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180238
display id: annotation2180238
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180238/range2180238/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180238/range2180238/1
type: Range
persistent identity: range2180238
display id: range2180238
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 4361
end: 5221
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2180237
display id: annotation2180237
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180237/range2180237/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180237/range2180237/1
type: Range
persistent identity: range2180237
display id: range2180237
version: 1
direction: direction/forward
top level: pSBBs1C
start: 1379
end: 2407
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2180236
display id: annotation2180236
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180236/range2180236/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180236/range2180236/1
type: Range
persistent identity: range2180236
display id: range2180236
version: 1
direction: direction/forward
top level: pSBBs1C
start: 358
end: 1008
orientation: inline

pSB3K3
type: ComponentDefinition
persistent identity: pSB3K3
display id: pSB3K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2004-06-28T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K3-1 is a medium copy plasmid with kanamycin resistance. It has a p15A pMR101-derived replication origin, copy number 20-30. (Lutz and Bujard, 1997) pSB3K3-1 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into the vectorl. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: kanR (rev)
sequence annotation: Prefix
sequence annotation: VF2
sequence annotation: p15A ORI (rev)
sequence annotation: terminator stem loop
sequence annotation: Suffix
sequence annotation: E. coli his operon terminator
sequence annotation: deletion of two bases
sequence annotation: VR
sequence annotation: end of terminator
sequence: https://synbiohub.org/public/igem/pSB3K3_sequence/1

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation1023045
display id: annotation1023045
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1
type: Range
persistent identity: range1023045
display id: range1023045
version: 1
direction: direction/reverse
top level: pSB3K3
start: 239
end: 1054
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1023043
display id: annotation1023043
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1
type: Range
persistent identity: range1023043
display id: range1023043
version: 1
direction: direction/forward
top level: pSB3K3
start: 2729
end: 2750
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782459
display id: annotation1782459
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1
type: Range
persistent identity: range1782459
display id: range1782459
version: 1
direction: direction/forward
top level: pSB3K3
start: 2611
end: 2630
orientation: inline

p15A ORI (rev)
type: SequenceAnnotation
persistent identity: annotation1023046
display id: annotation1023046
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1
type: Range
persistent identity: range1023046
display id: range1023046
version: 1
direction: direction/reverse
top level: pSB3K3
start: 1386
end: 2210
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824023
display id: annotation1824023
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1
type: Range
persistent identity: range1824023
display id: range1824023
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2700
end: 2719
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1023044
display id: annotation1023044
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1
type: Range
persistent identity: range1023044
display id: range1023044
version: 1
direction: direction/forward
top level: pSB3K3
start: 1
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824021
display id: annotation1824021
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1
type: Range
persistent identity: range1824021
display id: range1824021
version: 1
direction: direction/forward
top level: pSB3K3
start: 22
end: 93
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823788
display id: annotation1823788
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1

https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1
type: Range
persistent identity: range1823788
display id: range1823788
version: 1
direction: direction/forward
top level: pSB3K3
start: 34
end: 35
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782476
display id: annotation1782476
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1
type: Range
persistent identity: range1782476
display id: range1782476
version: 1
direction: direction/reverse
top level: pSB3K3
start: 157
end: 176
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824024
display id: annotation1824024
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1
type: Range
persistent identity: range1824024
display id: range1824024
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2687
end: 2690
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824022
display id: annotation1824022
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1
type: Range
persistent identity: range1824022
display id: range1824022
version: 1
direction: direction/forward
top level: pSB3K3
start: 30
end: 64
orientation: inline

BBa_K823055
type: ComponentDefinition
persistent identity: BBa K823055
display id: BBa_K823055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3F-Vector pSB1C3 with RFP-cassette in Freiburg Standard
creator: Jara Radeck
created: 2013-10-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To allow the possibility to save some nucleotides of primer-overhangs, you can use this vector. The two AgeI-restriction sites from the original RFP-cassette have been removed. The cassette is flanked by the modified Freiburg standard (http://2012.igem.org/wiki/images/b/b9/LMU-Munich_2012_Our_Freiburg_standard_FusionPrefix.pdf) that includes an optimal ribosome binding site between XbaI and NgoMIV restriction sites.
mutable notes: Two AgeI-restriction sites have been removed by two subsequent quikchange site-directed mutagnesis reactions. The RFP-cassette was PCR-amplified with the overhangs: prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
mutable provenance: pSB1C3; the RFP-part is originally from Discosoma sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ori (pMB1)
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K823055_sequence/1

ori (pMB1)
type: SequenceAnnotation
persistent identity: annotation2371772
display id: annotation2371772
version: 1
top level: BBa_K823055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823055/annotation2371772/range2371772/1

https://synbiohub.org/public/igem/BBa_K823055/annotation2371772/range2371772/1
type: Range
persistent identity: range2371772
display id: range2371772
version: 1
direction: direction/forward
top level: BBa_K823055
start: 260
end: 874
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2371771
display id: annotation2371771
version: 1
top level: BBa_K823055
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823055/annotation2371771/range2371771/1

https://synbiohub.org/public/igem/BBa_K823055/annotation2371771/range2371771/1
type: Range
persistent identity: range2371771
display id: range2371771
version: 1
direction: direction/reverse
top level: BBa_K823055
start: 1169
end: 1828
orientation: inline

pSB2K3
type: ComponentDefinition
persistent identity: pSB2K3
display id: pSB2K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible copy number BioBrick plasmid
creator: Sri Kosuri
created: 2004-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_48_46_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB2K3 is a variable copy number plasmid carrying kanamycin resistance. The plasmid contains the F' replication origin and also the P1 lytic replication origin. Genes encoding proteins that are required to activate the P1 lytic replication origin are carried on the plasmid under the control of a lacI-regulated promoter. When grown in cells producing LacI the plasmid is maintained at very low copy (<10 per cell). When induced with IPTG the P1 lytic replication proteins are produced and the P1 lytic replication origin takes over, raising the copy number to very high levels (>100++ per cell). The copy control features of pSB2K3 are useful for cloning "difficult" parts while still being able to prepare large amounts of DNA. Additionally, pSB2K3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.
mutable notes: *** pSCANS via BNL *** The pSB2 series plasmids are based on the pSCANS-5 plasmid from John Dunn's group at Brookhaven National Laboratory. The pSCANS plasmids are introduced here.
mutable provenance: MIT SBWG modification of pSCANS-5 from BNL
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator stem loop
sequence annotation: repL
sequence annotation: prefix
sequence annotation: suffix
sequence annotation: VR
sequence annotation: F1 Replication Origin
sequence annotation: kanR
sequence annotation: deletion of two bases
sequence annotation: nnpp promoters
sequence annotation: E. coli his operon terminator
sequence annotation: oriS Replication Origin (rev)
sequence annotation: lacOP
sequence annotation: VF2
sequence annotation: repE (rev)
sequence annotation: end of terminator
sequence: https://synbiohub.org/public/igem/pSB2K3_sequence/1

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824016
display id: annotation1824016
version: 1
top level: pSB2K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824016/range1824016/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824016/range1824016/1
type: Range
persistent identity: range1824016
display id: range1824016
version: 1
direction: direction/reverse
top level: pSB2K3
start: 4375
end: 4394
orientation: inline

repL
type: SequenceAnnotation
persistent identity: annotation1023027
display id: annotation1023027
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023027/range1023027/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023027/range1023027/1
type: Range
persistent identity: range1023027
display id: range1023027
version: 1
direction: direction/forward
top level: pSB2K3
start: 2289
end: 3134
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1023021
display id: annotation1023021
version: 1
top level: pSB2K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023021/range1023021/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023021/range1023021/1
type: Range
persistent identity: range1023021
display id: range1023021
version: 1
direction: direction/forward
top level: pSB2K3
start: 4404
end: 4425
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1023022
display id: annotation1023022
version: 1
top level: pSB2K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023022/range1023022/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023022/range1023022/1
type: Range
persistent identity: range1023022
display id: range1023022
version: 1
direction: direction/forward
top level: pSB2K3
start: 1
end: 21
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782483
display id: annotation1782483
version: 1
top level: pSB2K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K3/annotation1782483/range1782483/1

https://synbiohub.org/public/igem/pSB2K3/annotation1782483/range1782483/1
type: Range
persistent identity: range1782483
display id: range1782483
version: 1
direction: direction/reverse
top level: pSB2K3
start: 157
end: 176
orientation: inline

F1 Replication Origin
type: SequenceAnnotation
persistent identity: annotation1023023
display id: annotation1023023
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023023/range1023023/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023023/range1023023/1
type: Range
persistent identity: range1023023
display id: range1023023
version: 1
direction: direction/forward
top level: pSB2K3
start: 242
end: 290
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1023020
display id: annotation1023020
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023020/range1023020/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023020/range1023020/1
type: Range
persistent identity: range1023020
display id: range1023020
version: 1
direction: direction/forward
top level: pSB2K3
start: 3433
end: 4248
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823781
display id: annotation1823781
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1823781/range1823781/1

https://synbiohub.org/public/igem/pSB2K3/annotation1823781/range1823781/1
type: Range
persistent identity: range1823781
display id: range1823781
version: 1
direction: direction/forward
top level: pSB2K3
start: 34
end: 35
orientation: inline

nnpp promoters
type: SequenceAnnotation
persistent identity: annotation1023028
display id: annotation1023028
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023028/range1023028/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023028/range1023028/1
type: Range
persistent identity: range1023028
display id: range1023028
version: 1
direction: direction/forward
top level: pSB2K3
start: 3242
end: 3393
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824015
display id: annotation1824015
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824015/range1824015/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824015/range1824015/1
type: Range
persistent identity: range1824015
display id: range1824015
version: 1
direction: direction/forward
top level: pSB2K3
start: 22
end: 93
orientation: inline

oriS Replication Origin (rev)
type: SequenceAnnotation
persistent identity: annotation1023025
display id: annotation1023025
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023025/range1023025/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023025/range1023025/1
type: Range
persistent identity: range1023025
display id: range1023025
version: 1
direction: direction/reverse
top level: pSB2K3
start: 1463
end: 1679
orientation: inline

lacOP
type: SequenceAnnotation
persistent identity: annotation1023026
display id: annotation1023026
version: 1
top level: pSB2K3
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023026/range1023026/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023026/range1023026/1
type: Range
persistent identity: range1023026
display id: range1023026
version: 1
direction: direction/forward
top level: pSB2K3
start: 2037
end: 2211
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782484
display id: annotation1782484
version: 1
top level: pSB2K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K3/annotation1782484/range1782484/1

https://synbiohub.org/public/igem/pSB2K3/annotation1782484/range1782484/1
type: Range
persistent identity: range1782484
display id: range1782484
version: 1
direction: direction/forward
top level: pSB2K3
start: 4286
end: 4305
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824014
display id: annotation1824014
version: 1
top level: pSB2K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824014/range1824014/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824014/range1824014/1
type: Range
persistent identity: range1824014
display id: range1824014
version: 1
direction: direction/forward
top level: pSB2K3
start: 30
end: 64
orientation: inline

repE (rev)
type: SequenceAnnotation
persistent identity: annotation1023024
display id: annotation1023024
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023024/range1023024/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023024/range1023024/1
type: Range
persistent identity: range1023024
display id: range1023024
version: 1
direction: direction/reverse
top level: pSB2K3
start: 617
end: 1372
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824017
display id: annotation1824017
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824017/range1824017/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824017/range1824017/1
type: Range
persistent identity: range1824017
display id: range1824017
version: 1
direction: direction/reverse
top level: pSB2K3
start: 4362
end: 4365
orientation: inline

BBa_K864014
type: ComponentDefinition
persistent identity: BBa K864014
display id: BBa_K864014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864014_sequence/1

pSB8A15
type: ComponentDefinition
persistent identity: BBa K864016
display id: BBa_K864016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864016_sequence/1

BBa_K864015
type: ComponentDefinition
persistent identity: BBa K864015
display id: BBa_K864015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4S15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864015_sequence/1

pSB8S15FRT
type: ComponentDefinition
persistent identity: BBa K864023
display id: BBa_K864023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8S15(FRT) is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864023_sequence/1

BBa_K864002
type: ComponentDefinition
persistent identity: BBa K864002
display id: BBa_K864002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: KanR BBa_P1003
sequence annotation: rrnC terminator
sequence annotation: TonB terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: T3 terminator
sequence annotation: P22 late terminator
sequence annotation: VR
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: Lux terminator
sequence annotation: Lambda Red forward primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_K864002_sequence/1

KanR BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2198838
display id: annotation2198838
version: 1
top level: BBa_K864002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198838/range2198838/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198838/range2198838/1
type: Range
persistent identity: range2198838
display id: range2198838
version: 1
direction: direction/forward
top level: BBa_K864002
start: 2506
end: 3224
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198834
display id: annotation2198834
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198834/range2198834/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198834/range2198834/1
type: Range
persistent identity: range2198834
display id: range2198834
version: 1
direction: direction/forward
top level: BBa_K864002
start: 136
end: 175
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2198832
display id: annotation2198832
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198832/range2198832/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198832/range2198832/1
type: Range
persistent identity: range2198832
display id: range2198832
version: 1
direction: direction/forward
top level: BBa_K864002
start: 47
end: 115
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198831
display id: annotation2198831
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198831/range2198831/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198831/range2198831/1
type: Range
persistent identity: range2198831
display id: range2198831
version: 1
direction: direction/reverse
top level: BBa_K864002
start: 18
end: 38
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198841
display id: annotation2198841
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198841/range2198841/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198841/range2198841/1
type: Range
persistent identity: range2198841
display id: range2198841
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3539
end: 3616
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198839
display id: annotation2198839
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198839/range2198839/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198839/range2198839/1
type: Range
persistent identity: range2198839
display id: range2198839
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3478
end: 3518
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198833
display id: annotation2198833
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198833/range2198833/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198833/range2198833/1
type: Range
persistent identity: range2198833
display id: range2198833
version: 1
direction: direction/forward
top level: BBa_K864002
start: 116
end: 135
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2198835
display id: annotation2198835
version: 1
top level: BBa_K864002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198835/range2198835/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198835/range2198835/1
type: Range
persistent identity: range2198835
display id: range2198835
version: 1
direction: direction/forward
top level: BBa_K864002
start: 190
end: 2405
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198837
display id: annotation2198837
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198837/range2198837/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198837/range2198837/1
type: Range
persistent identity: range2198837
display id: range2198837
version: 1
direction: direction/reverse
top level: BBa_K864002
start: 2454
end: 2499
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198836
display id: annotation2198836
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198836/range2198836/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198836/range2198836/1
type: Range
persistent identity: range2198836
display id: range2198836
version: 1
direction: direction/forward
top level: BBa_K864002
start: 2433
end: 2450
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198840
display id: annotation2198840
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198840/range2198840/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198840/range2198840/1
type: Range
persistent identity: range2198840
display id: range2198840
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3519
end: 3538
orientation: inline

pSB3T5
type: ComponentDefinition
persistent identity: pSB3T5
display id: pSB3T5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3T5 is a low to medium copy plasmid based with tetracycline resistance. It has a p15A-derived replication origin. pSB3T5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the tetracycline resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G0002
component: BBa_G00001
component: BBa_P1005
component: BBa_G00100
component: BBa_B0062
component: BBa_G0004
component: BBa_B0042
component: BBa_B0054
component: BBa_G0003
component: BBa_B0053
component: BBa_I50032
component: BBa_B0043
component: BBa_B0055
component: BBa_B0044
component: BBa_G00000
component: BBa_G00102
sequence annotation: BBa_B0055
sequence annotation: BBa_B0042
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence annotation: BBa_G00001
sequence annotation: BBa_G00000
sequence annotation: BBa_G0003
sequence annotation: BBa_P1005
sequence annotation: BBa_G0002
sequence annotation: BBa_B0062
sequence annotation: BBa_I50032
sequence annotation: BBa_G00102
sequence annotation: BBa_G0004
sequence annotation: BBa_B0044
sequence annotation: BBa_B0054
sequence annotation: BBa_B0043
sequence: https://synbiohub.org/public/igem/pSB3T5_sequence/1

BBa_G0002
type: Component
persistent identity: component1928381
display id: component1928381
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G00001
type: Component
persistent identity: component1928358
display id: component1928358
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_P1005
type: Component
persistent identity: component1928384
display id: component1928384
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1005/1

BBa_G00100
type: Component
persistent identity: component1928388
display id: component1928388
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0062
type: Component
persistent identity: component1928376
display id: component1928376
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0004
type: Component
persistent identity: component1928385
display id: component1928385
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0042
type: Component
persistent identity: component1928370
display id: component1928370
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0054
type: Component
persistent identity: component1928373
display id: component1928373
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G0003
type: Component
persistent identity: component1928377
display id: component1928377
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0053
type: Component
persistent identity: component1928387
display id: component1928387
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_I50032
type: Component
persistent identity: component1928380
display id: component1928380
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_B0043
type: Component
persistent identity: component1928403
display id: component1928403
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: Component
persistent identity: component1928391
display id: component1928391
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0044
type: Component
persistent identity: component1928363
display id: component1928363
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00000
type: Component
persistent identity: component1928405
display id: component1928405
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00102
type: Component
persistent identity: component1928374
display id: component1928374
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component1928398
display id: component1928398
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1928391
display id: annotation1928391
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928391/range1928391/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3T5/annotation1928391/range1928391/1
type: Range
persistent identity: range1928391
display id: range1928391
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3128
end: 3205
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928398
display id: annotation1928398
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928398/range1928398/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3T5/annotation1928398/range1928398/1
type: Range
persistent identity: range1928398
display id: range1928398
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3206
end: 3217
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1928387
display id: annotation1928387
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928387/range1928387/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3T5/annotation1928387/range1928387/1
type: Range
persistent identity: range1928387
display id: range1928387
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3036
end: 3107
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1928388
display id: annotation1928388
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928388/range1928388/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3T5/annotation1928388/range1928388/1
type: Range
persistent identity: range1928388
display id: range1928388
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3108
end: 3127
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1928358
display id: annotation1928358
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928358/range1928358/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3T5/annotation1928358/range1928358/1
type: Range
persistent identity: range1928358
display id: range1928358
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1
end: 21
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1928405
display id: annotation1928405
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928405/range1928405/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3T5/annotation1928405/range1928405/1
type: Range
persistent identity: range1928405
display id: range1928405
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3231
end: 3252
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1928377
display id: annotation1928377
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928377/range1928377/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3T5/annotation1928377/range1928377/1
type: Range
persistent identity: range1928377
display id: range1928377
version: 1
direction: direction/reverse
top level: pSB3T5
start: 177
end: 183
orientation: inline

BBa_P1005
type: SequenceAnnotation
persistent identity: annotation1928384
display id: annotation1928384
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928384/range1928384/1
component: BBa_P1005

https://synbiohub.org/public/igem/pSB3T5/annotation1928384/range1928384/1
type: Range
persistent identity: range1928384
display id: range1928384
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1746
end: 3028
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1928381
display id: annotation1928381
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928381/range1928381/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3T5/annotation1928381/range1928381/1
type: Range
persistent identity: range1928381
display id: range1928381
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1738
end: 1745
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1928376
display id: annotation1928376
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928376/range1928376/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3T5/annotation1928376/range1928376/1
type: Range
persistent identity: range1928376
display id: range1928376
version: 1
direction: direction/reverse
top level: pSB3T5
start: 136
end: 176
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1928380
display id: annotation1928380
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928380/range1928380/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3T5/annotation1928380/range1928380/1
type: Range
persistent identity: range1928380
display id: range1928380
version: 1
direction: direction/reverse
top level: pSB3T5
start: 184
end: 1737
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1928374
display id: annotation1928374
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928374/range1928374/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3T5/annotation1928374/range1928374/1
type: Range
persistent identity: range1928374
display id: range1928374
version: 1
direction: direction/reverse
top level: pSB3T5
start: 116
end: 135
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1928385
display id: annotation1928385
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928385/range1928385/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3T5/annotation1928385/range1928385/1
type: Range
persistent identity: range1928385
display id: range1928385
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3029
end: 3035
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928370
display id: annotation1928370
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928370/range1928370/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3T5/annotation1928370/range1928370/1
type: Range
persistent identity: range1928370
display id: range1928370
version: 1
direction: direction/reverse
top level: pSB3T5
start: 35
end: 46
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1928363
display id: annotation1928363
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928363/range1928363/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3T5/annotation1928363/range1928363/1
type: Range
persistent identity: range1928363
display id: range1928363
version: 1
direction: direction/reverse
top level: pSB3T5
start: 22
end: 34
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1928373
display id: annotation1928373
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928373/range1928373/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3T5/annotation1928373/range1928373/1
type: Range
persistent identity: range1928373
display id: range1928373
version: 1
direction: direction/reverse
top level: pSB3T5
start: 47
end: 115
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1928403
display id: annotation1928403
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928403/range1928403/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3T5/annotation1928403/range1928403/1
type: Range
persistent identity: range1928403
display id: range1928403
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3218
end: 3230
orientation: inline

BBa_K864003
type: ComponentDefinition
persistent identity: BBa K864003
display id: BBa_K864003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Lambda Red reverse primer
sequence annotation: rnC terminator
sequence annotation: Lambda Red forward primer
sequence annotation: Promoter
sequence annotation: spcR
sequence annotation: Lux terminator
sequence annotation: P22 late terminator
sequence annotation: T3 terminator
sequence annotation: TonB terminator
sequence annotation: VF2
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_K864003_sequence/1

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2201234
display id: annotation2201234
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201234/range2201234/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201234/range2201234/1
type: Range
persistent identity: range2201234
display id: range2201234
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 18
end: 38
orientation: inline

rnC terminator
type: SequenceAnnotation
persistent identity: annotation2201237
display id: annotation2201237
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201237/range2201237/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201237/range2201237/1
type: Range
persistent identity: range2201237
display id: range2201237
version: 1
direction: direction/forward
top level: BBa_K864003
start: 136
end: 175
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2201239
display id: annotation2201239
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201239/range2201239/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201239/range2201239/1
type: Range
persistent identity: range2201239
display id: range2201239
version: 1
direction: direction/forward
top level: BBa_K864003
start: 2433
end: 2450
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2201242
display id: annotation2201242
version: 1
top level: BBa_K864003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201242/range2201242/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201242/range2201242/1
type: Range
persistent identity: range2201242
display id: range2201242
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 3575
end: 3601
orientation: inline

spcR
type: SequenceAnnotation
persistent identity: annotation2201241
display id: annotation2201241
version: 1
top level: BBa_K864003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201241/range2201241/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201241/range2201241/1
type: Range
persistent identity: range2201241
display id: range2201241
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 2510
end: 3508
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2201240
display id: annotation2201240
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201240/range2201240/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201240/range2201240/1
type: Range
persistent identity: range2201240
display id: range2201240
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 2454
end: 2499
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2201243
display id: annotation2201243
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201243/range2201243/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201243/range2201243/1
type: Range
persistent identity: range2201243
display id: range2201243
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3608
end: 3648
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2201245
display id: annotation2201245
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201245/range2201245/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201245/range2201245/1
type: Range
persistent identity: range2201245
display id: range2201245
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3669
end: 3746
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2201235
display id: annotation2201235
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201235/range2201235/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201235/range2201235/1
type: Range
persistent identity: range2201235
display id: range2201235
version: 1
direction: direction/forward
top level: BBa_K864003
start: 47
end: 115
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2201244
display id: annotation2201244
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201244/range2201244/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201244/range2201244/1
type: Range
persistent identity: range2201244
display id: range2201244
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3649
end: 3668
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2201238
display id: annotation2201238
version: 1
top level: BBa_K864003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201238/range2201238/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201238/range2201238/1
type: Range
persistent identity: range2201238
display id: range2201238
version: 1
direction: direction/forward
top level: BBa_K864003
start: 190
end: 2405
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2201236
display id: annotation2201236
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201236/range2201236/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201236/range2201236/1
type: Range
persistent identity: range2201236
display id: range2201236
version: 1
direction: direction/forward
top level: BBa_K864003
start: 116
end: 135
orientation: inline

K592200
type: ComponentDefinition
persistent identity: BBa K592200
display id: BBa_K592200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Erik Gullberg, Antonio Ascue Avalos, Erik Lundin
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 10133
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K592200 is a BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: To make the plasmid usable for Lambda Red recombineering, all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable provenance: Salmonella phage P22 late terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Translational stop BBa_B0042
sequence annotation: Terminator BBa_B0054
sequence annotation: VR site BBa_G00102
sequence annotation: p15A low to medium copy replication origin BBa_I50032
sequence annotation: Lambda Red R primer
sequence annotation: VF2 site BBa_G00100
sequence annotation: P22 late terminator
sequence annotation: SpcR promoter
sequence annotation: Terminator BBa_B0062
sequence annotation: Lambda red F primer
sequence annotation: Spectinomycin resistance marker
sequence annotation: T3 terminator BBa_B0055
sequence: https://synbiohub.org/public/igem/BBa_K592200_sequence/1

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2136380
display id: annotation2136380
version: 1
top level: K592200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136380/range2136380/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136380/range2136380/1
type: Range
persistent identity: range2136380
display id: range2136380
version: 1
direction: direction/forward
top level: K592200
start: 3091
end: 3102
orientation: inline

Terminator BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2136370
display id: annotation2136370
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136370/range2136370/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136370/range2136370/1
type: Range
persistent identity: range2136370
display id: range2136370
version: 1
direction: direction/forward
top level: K592200
start: 47
end: 115
orientation: inline

VR site BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2136371
display id: annotation2136371
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136371/range2136371/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136371/range2136371/1
type: Range
persistent identity: range2136371
display id: range2136371
version: 1
direction: direction/reverse
top level: K592200
start: 116
end: 135
orientation: inline

p15A low to medium copy replication origin BBa_I50032
type: SequenceAnnotation
persistent identity: annotation2136373
display id: annotation2136373
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136373/range2136373/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136373/range2136373/1
type: Range
persistent identity: range2136373
display id: range2136373
version: 1
direction: direction/forward
top level: K592200
start: 184
end: 1748
orientation: inline

Lambda Red R primer
type: SequenceAnnotation
persistent identity: annotation2136398
display id: annotation2136398
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136398/range2136398/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136398/range2136398/1
type: Range
persistent identity: range2136398
display id: range2136398
version: 1
direction: direction/reverse
top level: K592200
start: 20
end: 41
orientation: inline

VF2 site BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2136378
display id: annotation2136378
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136378/range2136378/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136378/range2136378/1
type: Range
persistent identity: range2136378
display id: range2136378
version: 1
direction: direction/forward
top level: K592200
start: 2993
end: 3012
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2136377
display id: annotation2136377
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136377/range2136377/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136377/range2136377/1
type: Range
persistent identity: range2136377
display id: range2136377
version: 1
direction: direction/forward
top level: K592200
start: 2952
end: 2992
orientation: inline

SpcR promoter
type: SequenceAnnotation
persistent identity: annotation2136376
display id: annotation2136376
version: 1
top level: K592200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136376/range2136376/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136376/range2136376/1
type: Range
persistent identity: range2136376
display id: range2136376
version: 1
direction: direction/reverse
top level: K592200
start: 2907
end: 2933
orientation: inline

Terminator BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2136372
display id: annotation2136372
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136372/range2136372/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136372/range2136372/1
type: Range
persistent identity: range2136372
display id: range2136372
version: 1
direction: direction/forward
top level: K592200
start: 136
end: 176
orientation: inline

Lambda red F primer
type: SequenceAnnotation
persistent identity: annotation2136374
display id: annotation2136374
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136374/range2136374/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136374/range2136374/1
type: Range
persistent identity: range2136374
display id: range2136374
version: 1
direction: direction/forward
top level: K592200
start: 1726
end: 1745
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2136369
display id: annotation2136369
version: 1
top level: K592200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136369/range2136369/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136369/range2136369/1
type: Range
persistent identity: range2136369
display id: range2136369
version: 1
direction: direction/forward
top level: K592200
start: 35
end: 46
orientation: inline

Spectinomycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2136375
display id: annotation2136375
version: 1
top level: K592200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136375/range2136375/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136375/range2136375/1
type: Range
persistent identity: range2136375
display id: range2136375
version: 1
direction: direction/reverse
top level: K592200
start: 1841
end: 2840
orientation: inline

T3 terminator BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2136379
display id: annotation2136379
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136379/range2136379/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136379/range2136379/1
type: Range
persistent identity: range2136379
display id: range2136379
version: 1
direction: direction/forward
top level: K592200
start: 3013
end: 3090
orientation: inline

pSB1AT3
type: ComponentDefinition
persistent identity: pSB1AT3
display id: pSB1AT3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Tom Knight
created: 2005-06-06T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 42
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
mutable provenance: pSB1A3 with added Tet resistance
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: deletion of two bases
sequence annotation: APr (rev)
sequence annotation: Prefix
sequence annotation: Suffix
sequence annotation: rep (pMB1) (rev)
sequence annotation: VR
sequence annotation: Tet-r
sequence annotation: E. coli his operon terminator
sequence annotation: terminator stem loop
sequence annotation: VF2
sequence annotation: ORI
sequence annotation: end of terminator
sequence: https://synbiohub.org/public/igem/pSB1AT3_sequence/1

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823801
display id: annotation1823801
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1823801/range1823801/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1823801/range1823801/1
type: Range
persistent identity: range1823801
display id: range1823801
version: 1
direction: direction/forward
top level: pSB1AT3
start: 34
end: 35
orientation: inline

APr (rev)
type: SequenceAnnotation
persistent identity: annotation1780314
display id: annotation1780314
version: 1
top level: pSB1AT3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780314/range1780314/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780314/range1780314/1
type: Range
persistent identity: range1780314
display id: range1780314
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 2324
end: 3184
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1780064
display id: annotation1780064
version: 1
top level: pSB1AT3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780064/range1780064/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780064/range1780064/1
type: Range
persistent identity: range1780064
display id: range1780064
version: 1
direction: direction/forward
top level: pSB1AT3
start: 3425
end: 3446
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1780046
display id: annotation1780046
version: 1
top level: pSB1AT3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780046/range1780046/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780046/range1780046/1
type: Range
persistent identity: range1780046
display id: range1780046
version: 1
direction: direction/forward
top level: pSB1AT3
start: 1
end: 21
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1780066
display id: annotation1780066
version: 1
top level: pSB1AT3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780066/range1780066/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780066/range1780066/1
type: Range
persistent identity: range1780066
display id: range1780066
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 261
end: 875
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1780316
display id: annotation1780316
version: 1
top level: pSB1AT3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780316/range1780316/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780316/range1780316/1
type: Range
persistent identity: range1780316
display id: range1780316
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 157
end: 176
orientation: inline

Tet-r
type: SequenceAnnotation
persistent identity: annotation1780313
display id: annotation1780313
version: 1
top level: pSB1AT3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780313/range1780313/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780313/range1780313/1
type: Range
persistent identity: range1780313
display id: range1780313
version: 1
direction: direction/forward
top level: pSB1AT3
start: 1127
end: 2317
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824009
display id: annotation1824009
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1824009/range1824009/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1824009/range1824009/1
type: Range
persistent identity: range1824009
display id: range1824009
version: 1
direction: direction/forward
top level: pSB1AT3
start: 22
end: 93
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780328
display id: annotation1780328
version: 1
top level: pSB1AT3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780328/range1780328/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780328/range1780328/1
type: Range
persistent identity: range1780328
display id: range1780328
version: 1
direction: direction/forward
top level: pSB1AT3
start: 30
end: 64
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780318
display id: annotation1780318
version: 1
top level: pSB1AT3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780318/range1780318/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780318/range1780318/1
type: Range
persistent identity: range1780318
display id: range1780318
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 3396
end: 3415
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1780317
display id: annotation1780317
version: 1
top level: pSB1AT3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780317/range1780317/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780317/range1780317/1
type: Range
persistent identity: range1780317
display id: range1780317
version: 1
direction: direction/forward
top level: pSB1AT3
start: 3307
end: 3326
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1780315
display id: annotation1780315
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780315/range1780315/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780315/range1780315/1
type: Range
persistent identity: range1780315
display id: range1780315
version: 1
direction: direction/forward
top level: pSB1AT3
start: 276
end: 276
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824010
display id: annotation1824010
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1824010/range1824010/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1824010/range1824010/1
type: Range
persistent identity: range1824010
display id: range1824010
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 3383
end: 3386
orientation: inline

pSB1A30
type: ComponentDefinition
persistent identity: BBa K1362091
display id: BBa_K1362091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A30: High copy BioBrick cloning/expression backbone carrying Amp resitance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VF2
sequence annotation: suffix
sequence annotation: end of terminator
sequence annotation: rep(pMB1) (rev)
sequence annotation: T7 promotor
sequence annotation: ampR (rev)
sequence annotation: terminator stem loop
sequence annotation: prefix
sequence annotation: VR
sequence annotation: E. coli his operon terminator
sequence annotation: lacO reg
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_K1362091_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation2404608
display id: annotation2404608
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1
type: Range
persistent identity: range2404608
display id: range2404608
version: 1
direction: direction/forward
top level: pSB1A30
start: 2018
end: 2037
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2404600
display id: annotation2404600
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1
type: Range
persistent identity: range2404600
display id: range2404600
version: 1
direction: direction/forward
top level: pSB1A30
start: 1
end: 21
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2404609
display id: annotation2404609
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1
type: Range
persistent identity: range2404609
display id: range2404609
version: 1
direction: direction/reverse
top level: pSB1A30
start: 2094
end: 2097
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2404605
display id: annotation2404605
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1
type: Range
persistent identity: range2404605
display id: range2404605
version: 1
direction: direction/reverse
top level: pSB1A30
start: 261
end: 875
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2404611
display id: annotation2404611
version: 1
top level: pSB1A30
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1
type: Range
persistent identity: range2404611
display id: range2404611
version: 1
direction: direction/forward
top level: pSB1A30
start: 2139
end: 2157
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation2404607
display id: annotation2404607
version: 1
top level: pSB1A30
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1
type: Range
persistent identity: range2404607
display id: range2404607
version: 1
direction: direction/reverse
top level: pSB1A30
start: 1035
end: 1895
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404603
display id: annotation2404603
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1
type: Range
persistent identity: range2404603
display id: range2404603
version: 1
direction: direction/forward
top level: pSB1A30
start: 30
end: 64
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2404632
display id: annotation2404632
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1
type: Range
persistent identity: range2404632
display id: range2404632
version: 1
direction: direction/forward
top level: pSB1A30
start: 2185
end: 2206
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404610
display id: annotation2404610
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1
type: Range
persistent identity: range2404610
display id: range2404610
version: 1
direction: direction/forward
top level: pSB1A30
start: 2107
end: 2126
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2404602
display id: annotation2404602
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1
type: Range
persistent identity: range2404602
display id: range2404602
version: 1
direction: direction/reverse
top level: pSB1A30
start: 157
end: 176
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2404601
display id: annotation2404601
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1
type: Range
persistent identity: range2404601
display id: range2404601
version: 1
direction: direction/forward
top level: pSB1A30
start: 22
end: 93
orientation: inline

lacO reg
type: SequenceAnnotation
persistent identity: annotation2404631
display id: annotation2404631
version: 1
top level: pSB1A30
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1
type: Range
persistent identity: range2404631
display id: range2404631
version: 1
direction: direction/forward
top level: pSB1A30
start: 2157
end: 2184
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2404606
display id: annotation2404606
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1
type: Range
persistent identity: range2404606
display id: range2404606
version: 1
direction: direction/forward
top level: pSB1A30
start: 276
end: 276
orientation: inline

pSB1A7
type: ComponentDefinition
persistent identity: pSB1A7
display id: pSB1A7
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transcriptionally insulated high copy BioBrick plasmid
creator: Karmella Haynes
created: 2006-10-18T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pSB1A4 was constructed by adding double terminators ([BBa_B0015]) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. This insulator blocks read-through transcription into BioBrick parts/ devices.
mutable notes: The double terminators were assembled from small smaller overlapping ssDNA oligos. The outer-most EcoRI and PstI sites were mutated while the inner-most BioBrick cut sites were restored.
mutable provenance: Based upon pSB1A3; Includes two copies of [BBa_B0015] assembled from DNA oligos
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: B0015
sequence annotation: c > g
sequence annotation: stem_loop
sequence annotation: prefix
sequence annotation: E. coli his operon terminator
sequence annotation: mutated EcoRI
sequence annotation: end of terminator
sequence annotation: mutated PstI
sequence annotation: VF2
sequence annotation: ampR (rev)
sequence annotation: VR
sequence annotation: suffix
sequence annotation: rep(pMB1) (rev)
sequence annotation: B0015 (rev)
sequence: https://synbiohub.org/public/igem/pSB1A7_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation1903658
display id: annotation1903658
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903658/range1903658/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903658/range1903658/1
type: Range
persistent identity: range1903658
display id: range1903658
version: 1
direction: direction/forward
top level: pSB1A7
start: 2279
end: 2407
orientation: inline

c > g
type: SequenceAnnotation
persistent identity: annotation1903647
display id: annotation1903647
version: 1
top level: pSB1A7
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903647/range1903647/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903647/range1903647/1
type: Range
persistent identity: range1903647
display id: range1903647
version: 1
direction: direction/forward
top level: pSB1A7
start: 153
end: 153
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903645
display id: annotation1903645
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903645/range1903645/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903645/range1903645/1
type: Range
persistent identity: range1903645
display id: range1903645
version: 1
direction: direction/reverse
top level: pSB1A7
start: 38
end: 57
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1903661
display id: annotation1903661
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903661/range1903661/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903661/range1903661/1
type: Range
persistent identity: range1903661
display id: range1903661
version: 1
direction: direction/forward
top level: pSB1A7
start: 2411
end: 2431
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1903649
display id: annotation1903649
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903649/range1903649/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903649/range1903649/1
type: Range
persistent identity: range1903649
display id: range1903649
version: 1
direction: direction/forward
top level: pSB1A7
start: 159
end: 229
orientation: inline

mutated EcoRI
type: SequenceAnnotation
persistent identity: annotation1903656
display id: annotation1903656
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903656/range1903656/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903656/range1903656/1
type: Range
persistent identity: range1903656
display id: range1903656
version: 1
direction: direction/forward
top level: pSB1A7
start: 2273
end: 2278
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903659
display id: annotation1903659
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903659/range1903659/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903659/range1903659/1
type: Range
persistent identity: range1903659
display id: range1903659
version: 1
direction: direction/forward
top level: pSB1A7
start: 2290
end: 2333
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1903654
display id: annotation1903654
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903654/range1903654/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903654/range1903654/1
type: Range
persistent identity: range1903654
display id: range1903654
version: 1
direction: direction/forward
top level: pSB1A7
start: 2231
end: 2234
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903655
display id: annotation1903655
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903655/range1903655/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903655/range1903655/1
type: Range
persistent identity: range1903655
display id: range1903655
version: 1
direction: direction/forward
top level: pSB1A7
start: 2244
end: 2263
orientation: inline

mutated PstI
type: SequenceAnnotation
persistent identity: annotation1903648
display id: annotation1903648
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903648/range1903648/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903648/range1903648/1
type: Range
persistent identity: range1903648
display id: range1903648
version: 1
direction: direction/forward
top level: pSB1A7
start: 153
end: 158
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1903643
display id: annotation1903643
version: 1
top level: pSB1A7
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903643/range1903643/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903643/range1903643/1
type: Range
persistent identity: range1903643
display id: range1903643
version: 1
direction: direction/forward
top level: pSB1A7
start: 2155
end: 2174
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation1903653
display id: annotation1903653
version: 1
top level: pSB1A7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903653/range1903653/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903653/range1903653/1
type: Range
persistent identity: range1903653
display id: range1903653
version: 1
direction: direction/reverse
top level: pSB1A7
start: 1172
end: 2032
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903650
display id: annotation1903650
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903650/range1903650/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903650/range1903650/1
type: Range
persistent identity: range1903650
display id: range1903650
version: 1
direction: direction/forward
top level: pSB1A7
start: 167
end: 201
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903660
display id: annotation1903660
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903660/range1903660/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903660/range1903660/1
type: Range
persistent identity: range1903660
display id: range1903660
version: 1
direction: direction/forward
top level: pSB1A7
start: 2374
end: 2393
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1903651
display id: annotation1903651
version: 1
top level: pSB1A7
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903651/range1903651/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903651/range1903651/1
type: Range
persistent identity: range1903651
display id: range1903651
version: 1
direction: direction/reverse
top level: pSB1A7
start: 294
end: 313
orientation: inline

c > g
type: SequenceAnnotation
persistent identity: annotation1903657
display id: annotation1903657
version: 1
top level: pSB1A7
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903657/range1903657/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903657/range1903657/1
type: Range
persistent identity: range1903657
display id: range1903657
version: 1
direction: direction/forward
top level: pSB1A7
start: 2278
end: 2278
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903646
display id: annotation1903646
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903646/range1903646/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903646/range1903646/1
type: Range
persistent identity: range1903646
display id: range1903646
version: 1
direction: direction/reverse
top level: pSB1A7
start: 98
end: 141
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1903642
display id: annotation1903642
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903642/range1903642/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903642/range1903642/1
type: Range
persistent identity: range1903642
display id: range1903642
version: 1
direction: direction/forward
top level: pSB1A7
start: 1
end: 20
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1903652
display id: annotation1903652
version: 1
top level: pSB1A7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903652/range1903652/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903652/range1903652/1
type: Range
persistent identity: range1903652
display id: range1903652
version: 1
direction: direction/reverse
top level: pSB1A7
start: 398
end: 1012
orientation: inline

B0015 (rev)
type: SequenceAnnotation
persistent identity: annotation1903644
display id: annotation1903644
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903644/range1903644/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903644/range1903644/1
type: Range
persistent identity: range1903644
display id: range1903644
version: 1
direction: direction/reverse
top level: pSB1A7
start: 24
end: 152
orientation: inline

pSB8A15FRT
type: ComponentDefinition
persistent identity: BBa K864020
display id: BBa_K864020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864020_sequence/1

pSB3K5
type: ComponentDefinition
persistent identity: pSB3K5
display id: pSB3K5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-05-02T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K5 is a low to medium copy plasmid based with kanamycin resistance. It has a p15A-derived replication origin. pSB3K5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the kanamycin resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00100
component: BBa_P1003
component: BBa_G0004
component: BBa_G0002
component: BBa_G00102
component: BBa_B0062
component: BBa_B0044
component: BBa_B0042
component: BBa_G00001
component: BBa_B0053
component: BBa_I50032
component: BBa_G00000
component: BBa_B0054
component: BBa_B0043
component: BBa_B0055
component: BBa_G0003
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_B0062
sequence annotation: BBa_B0044
sequence annotation: BBa_G0002
sequence annotation: BBa_G00000
sequence annotation: BBa_G00100
sequence annotation: BBa_B0043
sequence annotation: BBa_B0053
sequence annotation: BBa_G0003
sequence annotation: BBa_G00001
sequence annotation: BBa_P1003
sequence annotation: BBa_G0004
sequence annotation: BBa_I50032
sequence annotation: BBa_B0055
sequence annotation: BBa_G00102
sequence: https://synbiohub.org/public/igem/pSB3K5_sequence/1

BBa_G00100
type: Component
persistent identity: component1932107
display id: component1932107
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_P1003
type: Component
persistent identity: component1932103
display id: component1932103
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1003/1

BBa_G0004
type: Component
persistent identity: component1932104
display id: component1932104
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G0002
type: Component
persistent identity: component1932101
display id: component1932101
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G00102
type: Component
persistent identity: component1932094
display id: component1932094
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0062
type: Component
persistent identity: component1932096
display id: component1932096
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0044
type: Component
persistent identity: component1932083
display id: component1932083
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0042
type: Component
persistent identity: component1932090
display id: component1932090
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00001
type: Component
persistent identity: component1932078
display id: component1932078
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0053
type: Component
persistent identity: component1932106
display id: component1932106
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_I50032
type: Component
persistent identity: component1932100
display id: component1932100
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_G00000
type: Component
persistent identity: component1932124
display id: component1932124
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0054
type: Component
persistent identity: component1932093
display id: component1932093
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0043
type: Component
persistent identity: component1932122
display id: component1932122
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: Component
persistent identity: component1932110
display id: component1932110
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G0003
type: Component
persistent identity: component1932097
display id: component1932097
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0042
type: Component
persistent identity: component1932117
display id: component1932117
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1932117
display id: annotation1932117
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932117/range1932117/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3K5/annotation1932117/range1932117/1
type: Range
persistent identity: range1932117
display id: range1932117
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2890
end: 2901
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1932093
display id: annotation1932093
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932093/range1932093/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3K5/annotation1932093/range1932093/1
type: Range
persistent identity: range1932093
display id: range1932093
version: 1
direction: direction/reverse
top level: pSB3K5
start: 47
end: 115
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1932096
display id: annotation1932096
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932096/range1932096/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3K5/annotation1932096/range1932096/1
type: Range
persistent identity: range1932096
display id: range1932096
version: 1
direction: direction/reverse
top level: pSB3K5
start: 136
end: 176
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1932083
display id: annotation1932083
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932083/range1932083/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3K5/annotation1932083/range1932083/1
type: Range
persistent identity: range1932083
display id: range1932083
version: 1
direction: direction/reverse
top level: pSB3K5
start: 22
end: 34
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1932101
display id: annotation1932101
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932101/range1932101/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3K5/annotation1932101/range1932101/1
type: Range
persistent identity: range1932101
display id: range1932101
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1738
end: 1745
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1932124
display id: annotation1932124
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932124/range1932124/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3K5/annotation1932124/range1932124/1
type: Range
persistent identity: range1932124
display id: range1932124
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2915
end: 2936
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1932107
display id: annotation1932107
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932107/range1932107/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3K5/annotation1932107/range1932107/1
type: Range
persistent identity: range1932107
display id: range1932107
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2792
end: 2811
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1932122
display id: annotation1932122
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932122/range1932122/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3K5/annotation1932122/range1932122/1
type: Range
persistent identity: range1932122
display id: range1932122
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2902
end: 2914
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1932106
display id: annotation1932106
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932106/range1932106/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3K5/annotation1932106/range1932106/1
type: Range
persistent identity: range1932106
display id: range1932106
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2720
end: 2791
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1932097
display id: annotation1932097
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932097/range1932097/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3K5/annotation1932097/range1932097/1
type: Range
persistent identity: range1932097
display id: range1932097
version: 1
direction: direction/reverse
top level: pSB3K5
start: 177
end: 183
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1932078
display id: annotation1932078
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932078/range1932078/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3K5/annotation1932078/range1932078/1
type: Range
persistent identity: range1932078
display id: range1932078
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1
end: 21
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation1932103
display id: annotation1932103
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932103/range1932103/1
component: BBa_P1003

https://synbiohub.org/public/igem/pSB3K5/annotation1932103/range1932103/1
type: Range
persistent identity: range1932103
display id: range1932103
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1746
end: 2712
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1932104
display id: annotation1932104
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932104/range1932104/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3K5/annotation1932104/range1932104/1
type: Range
persistent identity: range1932104
display id: range1932104
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2713
end: 2719
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1932100
display id: annotation1932100
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932100/range1932100/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3K5/annotation1932100/range1932100/1
type: Range
persistent identity: range1932100
display id: range1932100
version: 1
direction: direction/reverse
top level: pSB3K5
start: 184
end: 1737
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1932110
display id: annotation1932110
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932110/range1932110/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3K5/annotation1932110/range1932110/1
type: Range
persistent identity: range1932110
display id: range1932110
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2812
end: 2889
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1932094
display id: annotation1932094
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932094/range1932094/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3K5/annotation1932094/range1932094/1
type: Range
persistent identity: range1932094
display id: range1932094
version: 1
direction: direction/reverse
top level: pSB3K5
start: 116
end: 135
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1932090
display id: annotation1932090
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932090/range1932090/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3K5/annotation1932090/range1932090/1
type: Range
persistent identity: range1932090
display id: range1932090
version: 1
direction: direction/reverse
top level: pSB3K5
start: 35
end: 46
orientation: inline

pSB1K3
type: ComponentDefinition
persistent identity: pSB1K3
display id: pSB1K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Austin Che
created: 2008-09-07T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.
mutable notes: Eliminated ampicillin resistance.
mutable provenance: This plasmid was derived from pSB1AK3-P1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick suffix
sequence annotation: end of terminator
sequence annotation: E. coli his operon terminator
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: repeat region
sequence annotation: ORI
sequence annotation: rep (pMB1)
sequence annotation: G->C mutation to remove XhoI site
sequence annotation: BioBrick prefix
sequence annotation: Stem loop
sequence annotation: Kanamycin resistance marker
sequence annotation: Verification reverse (VR) primer binding site
sequence: https://synbiohub.org/public/igem/pSB1K3_sequence/1

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2120009
display id: annotation2120009
version: 1
top level: pSB1K3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120009/range2120009/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120009/range2120009/1
type: Range
persistent identity: range2120009
display id: range2120009
version: 1
direction: direction/forward
top level: pSB1K3
start: 1
end: 21
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2120019
display id: annotation2120019
version: 1
top level: pSB1K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120019/range2120019/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120019/range2120019/1
type: Range
persistent identity: range2120019
display id: range2120019
version: 1
direction: direction/reverse
top level: pSB1K3
start: 2143
end: 2146
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2120010
display id: annotation2120010
version: 1
top level: pSB1K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120010/range2120010/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120010/range2120010/1
type: Range
persistent identity: range2120010
display id: range2120010
version: 1
direction: direction/forward
top level: pSB1K3
start: 22
end: 93
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation2120018
display id: annotation2120018
version: 1
top level: pSB1K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120018/range2120018/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120018/range2120018/1
type: Range
persistent identity: range2120018
display id: range2120018
version: 1
direction: direction/forward
top level: pSB1K3
start: 2067
end: 2086
orientation: inline

repeat region
type: SequenceAnnotation
persistent identity: annotation2120017
display id: annotation2120017
version: 1
top level: pSB1K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120017/range2120017/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120017/range2120017/1
type: Range
persistent identity: range2120017
display id: range2120017
version: 1
direction: direction/reverse
top level: pSB1K3
start: 2017
end: 2060
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2120014
display id: annotation2120014
version: 1
top level: pSB1K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120014/range2120014/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120014/range2120014/1
type: Range
persistent identity: range2120014
display id: range2120014
version: 1
direction: direction/forward
top level: pSB1K3
start: 276
end: 276
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2120013
display id: annotation2120013
version: 1
top level: pSB1K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120013/range2120013/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120013/range2120013/1
type: Range
persistent identity: range2120013
display id: range2120013
version: 1
direction: direction/reverse
top level: pSB1K3
start: 261
end: 875
orientation: inline

G->C mutation to remove XhoI site
type: SequenceAnnotation
persistent identity: annotation2120016
display id: annotation2120016
version: 1
top level: pSB1K3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120016/range2120016/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120016/range2120016/1
type: Range
persistent identity: range2120016
display id: range2120016
version: 1
direction: direction/reverse
top level: pSB1K3
start: 1880
end: 1880
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2120021
display id: annotation2120021
version: 1
top level: pSB1K3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120021/range2120021/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120021/range2120021/1
type: Range
persistent identity: range2120021
display id: range2120021
version: 1
direction: direction/forward
top level: pSB1K3
start: 2185
end: 2206
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2120011
display id: annotation2120011
version: 1
top level: pSB1K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120011/range2120011/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120011/range2120011/1
type: Range
persistent identity: range2120011
display id: range2120011
version: 1
direction: direction/forward
top level: pSB1K3
start: 30
end: 64
orientation: inline

Kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2120015
display id: annotation2120015
version: 1
top level: pSB1K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120015/range2120015/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120015/range2120015/1
type: Range
persistent identity: range2120015
display id: range2120015
version: 1
direction: direction/reverse
top level: pSB1K3
start: 1097
end: 1912
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2120020
display id: annotation2120020
version: 1
top level: pSB1K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120020/range2120020/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120020/range2120020/1
type: Range
persistent identity: range2120020
display id: range2120020
version: 1
direction: direction/reverse
top level: pSB1K3
start: 2156
end: 2175
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation2120012
display id: annotation2120012
version: 1
top level: pSB1K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120012/range2120012/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120012/range2120012/1
type: Range
persistent identity: range2120012
display id: range2120012
version: 1
direction: direction/reverse
top level: pSB1K3
start: 157
end: 176
orientation: inline

BBa_J179000
type: ComponentDefinition
persistent identity: BBa J179000
display id: BBa_J179000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBS1C: Bacillus subtilis vector, amyE integration, CAM-resistance
creator: Jara Radeck
created: 2013-12-03T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This vector integrates into the amyE-site of Bacillus subtilis via double recombination. The two recombination sites flank a standard BioBrick multiple cloning site (with RFP-cassette), as well as a chloramphenicol resistance cassette (5 ??g/ml). The backbone does not contain the restriction sites EcoRI, XbaI, SpeI, PstI, NgoMIV nor AgeI. The vector is replicative in Escherichia coli and provides resistance to ampicillin (100 ??g/ml). Before transformation into B. subtilis, this vector should be linearized with ScaI.
mutable notes: This is one of three empty vectors with compatible integration loci and resistance cassettes to allow combination of up to three constructs of choice in one single strain.
mutable provenance: pDG1731
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: cat
sequence annotation: amyE-front
sequence annotation: amyE-back
sequence annotation: bla
sequence annotation: ori
sequence: https://synbiohub.org/public/igem/BBa_J179000_sequence/1

cat
type: SequenceAnnotation
persistent identity: annotation2371573
display id: annotation2371573
version: 1
top level: BBa_J179000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371573/range2371573/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371573/range2371573/1
type: Range
persistent identity: range2371573
display id: range2371573
version: 1
direction: direction/forward
top level: BBa_J179000
start: 358
end: 1008
orientation: inline

amyE-front
type: SequenceAnnotation
persistent identity: annotation2371576
display id: annotation2371576
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371576/range2371576/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371576/range2371576/1
type: Range
persistent identity: range2371576
display id: range2371576
version: 1
direction: direction/forward
top level: BBa_J179000
start: 5546
end: 6066
orientation: inline

amyE-back
type: SequenceAnnotation
persistent identity: annotation2371574
display id: annotation2371574
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371574/range2371574/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371574/range2371574/1
type: Range
persistent identity: range2371574
display id: range2371574
version: 1
direction: direction/forward
top level: BBa_J179000
start: 1379
end: 2407
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371575
display id: annotation2371575
version: 1
top level: BBa_J179000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371575/range2371575/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371575/range2371575/1
type: Range
persistent identity: range2371575
display id: range2371575
version: 1
direction: direction/reverse
top level: BBa_J179000
start: 4361
end: 5221
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2371577
display id: annotation2371577
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371577/range2371577/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371577/range2371577/1
type: Range
persistent identity: range2371577
display id: range2371577
version: 1
direction: direction/forward
top level: BBa_J179000
start: 3587
end: 4201
orientation: inline

pSB8C15
type: ComponentDefinition
persistent identity: BBa K864017
display id: BBa_K864017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8C15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and Chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Lux terminator
sequence annotation: VF2 forward primer binding site
sequence annotation: VR reverse primer binding site
sequence annotation: tonB terminator
sequence annotation: Promoter
sequence annotation: T3 terminator
sequence annotation: -35
sequence annotation: -10
sequence annotation: pSC101-ts ori
sequence annotation: cmR
sequence annotation: rrnC
sequence annotation: P22 late terminator
sequence: https://synbiohub.org/public/igem/BBa_K864017_sequence/1

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2203159
display id: annotation2203159
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203159/range2203159/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203159/range2203159/1
type: Range
persistent identity: range2203159
display id: range2203159
version: 1
direction: direction/reverse
top level: pSB8C15
start: 2454
end: 2499
orientation: inline

VF2 forward primer binding site
type: SequenceAnnotation
persistent identity: annotation2203154
display id: annotation2203154
version: 1
top level: pSB8C15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203154/range2203154/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203154/range2203154/1
type: Range
persistent identity: range2203154
display id: range2203154
version: 1
direction: direction/forward
top level: pSB8C15
start: 3322
end: 3341
orientation: inline

VR reverse primer binding site
type: SequenceAnnotation
persistent identity: annotation2203046
display id: annotation2203046
version: 1
top level: pSB8C15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203046/range2203046/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203046/range2203046/1
type: Range
persistent identity: range2203046
display id: range2203046
version: 1
direction: direction/reverse
top level: pSB8C15
start: 116
end: 135
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2203157
display id: annotation2203157
version: 1
top level: pSB8C15
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203157/range2203157/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203157/range2203157/1
type: Range
persistent identity: range2203157
display id: range2203157
version: 1
direction: direction/forward
top level: pSB8C15
start: 47
end: 115
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2202888
display id: annotation2202888
version: 1
top level: pSB8C15
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2202888/range2202888/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2202888/range2202888/1
type: Range
persistent identity: range2202888
display id: range2202888
version: 1
direction: direction/reverse
top level: pSB8C15
start: 3235
end: 3461
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2203161
display id: annotation2203161
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203161/range2203161/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203161/range2203161/1
type: Range
persistent identity: range2203161
display id: range2203161
version: 1
direction: direction/forward
top level: pSB8C15
start: 3342
end: 3419
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2203156
display id: annotation2203156
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203156/range2203156/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203156/range2203156/1
type: Range
persistent identity: range2203156
display id: range2203156
version: 1
direction: direction/forward
top level: pSB8C15
start: 881
end: 886
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2203162
display id: annotation2203162
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203162/range2203162/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203162/range2203162/1
type: Range
persistent identity: range2203162
display id: range2203162
version: 1
direction: direction/forward
top level: pSB8C15
start: 903
end: 908
orientation: inline

pSC101-ts ori
type: SequenceAnnotation
persistent identity: annotation2203153
display id: annotation2203153
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203153/range2203153/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203153/range2203153/1
type: Range
persistent identity: range2203153
display id: range2203153
version: 1
direction: direction/forward
top level: pSB8C15
start: 190
end: 2405
orientation: inline

cmR
type: SequenceAnnotation
persistent identity: annotation2203155
display id: annotation2203155
version: 1
top level: pSB8C15
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203155/range2203155/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203155/range2203155/1
type: Range
persistent identity: range2203155
display id: range2203155
version: 1
direction: direction/reverse
top level: pSB8C15
start: 2506
end: 3168
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation2203158
display id: annotation2203158
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203158/range2203158/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203158/range2203158/1
type: Range
persistent identity: range2203158
display id: range2203158
version: 1
direction: direction/forward
top level: pSB8C15
start: 136
end: 175
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2203160
display id: annotation2203160
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203160/range2203160/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203160/range2203160/1
type: Range
persistent identity: range2203160
display id: range2203160
version: 1
direction: direction/forward
top level: pSB8C15
start: 3281
end: 3321
orientation: inline

pSB4C1
type: ComponentDefinition
persistent identity: pSB4C1
display id: pSB4C1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCK01 converted into BioBrick vector (formerly BBa_I739202)
creator: Stefan Luzi
created: 2007-10-23T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Mutagenesis primer
sequence annotation: BioBrick suffix
sequence annotation: chloramphenicol acetyltransferase
sequence annotation: BioBrick prefix
sequence annotation: pSC101 replication initiator protein
sequence annotation: C->T mutation to eliminate SpeI site
sequence: https://synbiohub.org/public/igem/pSB4C1_sequence/1

Mutagenesis primer
type: SequenceAnnotation
persistent identity: annotation1969635
display id: annotation1969635
version: 1
top level: pSB4C1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969635/range1969635/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969635/range1969635/1
type: Range
persistent identity: range1969635
display id: range1969635
version: 1
direction: direction/reverse
top level: pSB4C1
start: 1491
end: 1529
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969631
display id: annotation1969631
version: 1
top level: pSB4C1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969631/range1969631/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969631/range1969631/1
type: Range
persistent identity: range1969631
display id: range1969631
version: 1
direction: direction/forward
top level: pSB4C1
start: 1
end: 21
orientation: inline

chloramphenicol acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1969633
display id: annotation1969633
version: 1
top level: pSB4C1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969633/range1969633/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969633/range1969633/1
type: Range
persistent identity: range1969633
display id: range1969633
version: 1
direction: direction/forward
top level: pSB4C1
start: 2311
end: 2970
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969636
display id: annotation1969636
version: 1
top level: pSB4C1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969636/range1969636/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969636/range1969636/1
type: Range
persistent identity: range1969636
display id: range1969636
version: 1
direction: direction/forward
top level: pSB4C1
start: 3134
end: 3155
orientation: inline

pSC101 replication initiator protein
type: SequenceAnnotation
persistent identity: annotation1969632
display id: annotation1969632
version: 1
top level: pSB4C1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969632/range1969632/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969632/range1969632/1
type: Range
persistent identity: range1969632
display id: range1969632
version: 1
direction: direction/reverse
top level: pSB4C1
start: 490
end: 1440
orientation: inline

C->T mutation to eliminate SpeI site
type: SequenceAnnotation
persistent identity: annotation1969634
display id: annotation1969634
version: 1
top level: pSB4C1
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969634/range1969634/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969634/range1969634/1
type: Range
persistent identity: range1969634
display id: range1969634
version: 1
direction: direction/forward
top level: pSB4C1
start: 1508
end: 1508
orientation: inline

pSB8C15FRT
type: ComponentDefinition
persistent identity: BBa K864021
display id: BBa_K864021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864021_sequence/1

BBa_K864008
type: ComponentDefinition
persistent identity: BBa K864008
display id: BBa_K864008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864008_sequence/1

BBa_K864013
type: ComponentDefinition
persistent identity: BBa K864013
display id: BBa_K864013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4C15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864013_sequence/1

pSB8K15FRT
type: ComponentDefinition
persistent identity: BBa K864022
display id: BBa_K864022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: awd
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864022_sequence/1

pSB1AK3
type: ComponentDefinition
persistent identity: pSB1AK3
display id: pSB1AK3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Registry
created: 2004-07-27T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_48_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator stem loop
sequence annotation: E. coli his terminator
sequence annotation: kanR (rev)
sequence annotation: rep (pMB1) (rev)
sequence annotation: Prefix
sequence annotation: ampR (rev)
sequence annotation: deletion of two bases
sequence annotation: Suffix
sequence annotation: VF2
sequence annotation: ORI
sequence annotation: end of terminator
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/pSB1AK3_sequence/1

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780433
display id: annotation1780433
version: 1
top level: pSB1AK3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780433/range1780433/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780433/range1780433/1
type: Range
persistent identity: range1780433
display id: range1780433
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 3139
end: 3158
orientation: inline

E. coli his terminator
type: SequenceAnnotation
persistent identity: annotation1823992
display id: annotation1823992
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1823992/range1823992/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1823992/range1823992/1
type: Range
persistent identity: range1823992
display id: range1823992
version: 1
direction: direction/forward
top level: pSB1AK3
start: 22
end: 93
orientation: inline

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation1780430
display id: annotation1780430
version: 1
top level: pSB1AK3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780430/range1780430/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780430/range1780430/1
type: Range
persistent identity: range1780430
display id: range1780430
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 1097
end: 1912
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1780429
display id: annotation1780429
version: 1
top level: pSB1AK3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780429/range1780429/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780429/range1780429/1
type: Range
persistent identity: range1780429
display id: range1780429
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 261
end: 875
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1780401
display id: annotation1780401
version: 1
top level: pSB1AK3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780401/range1780401/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780401/range1780401/1
type: Range
persistent identity: range1780401
display id: range1780401
version: 1
direction: direction/forward
top level: pSB1AK3
start: 3168
end: 3189
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation1780431
display id: annotation1780431
version: 1
top level: pSB1AK3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780431/range1780431/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780431/range1780431/1
type: Range
persistent identity: range1780431
display id: range1780431
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 2067
end: 2927
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823805
display id: annotation1823805
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1823805/range1823805/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1823805/range1823805/1
type: Range
persistent identity: range1823805
display id: range1823805
version: 1
direction: direction/forward
top level: pSB1AK3
start: 34
end: 35
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1780400
display id: annotation1780400
version: 1
top level: pSB1AK3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780400/range1780400/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780400/range1780400/1
type: Range
persistent identity: range1780400
display id: range1780400
version: 1
direction: direction/forward
top level: pSB1AK3
start: 1
end: 21
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1780435
display id: annotation1780435
version: 1
top level: pSB1AK3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780435/range1780435/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780435/range1780435/1
type: Range
persistent identity: range1780435
display id: range1780435
version: 1
direction: direction/forward
top level: pSB1AK3
start: 3050
end: 3069
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1780432
display id: annotation1780432
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780432/range1780432/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780432/range1780432/1
type: Range
persistent identity: range1780432
display id: range1780432
version: 1
direction: direction/forward
top level: pSB1AK3
start: 276
end: 276
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1823993
display id: annotation1823993
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1823993/range1823993/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1823993/range1823993/1
type: Range
persistent identity: range1823993
display id: range1823993
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 3126
end: 3129
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780434
display id: annotation1780434
version: 1
top level: pSB1AK3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780434/range1780434/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780434/range1780434/1
type: Range
persistent identity: range1780434
display id: range1780434
version: 1
direction: direction/forward
top level: pSB1AK3
start: 30
end: 64
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1780436
display id: annotation1780436
version: 1
top level: pSB1AK3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780436/range1780436/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780436/range1780436/1
type: Range
persistent identity: range1780436
display id: range1780436
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 157
end: 176
orientation: inline

BBa_K864012
type: ComponentDefinition
persistent identity: BBa K864012
display id: BBa_K864012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864012_sequence/1

BBa_K864004
type: ComponentDefinition
persistent identity: BBa K864004
display id: BBa_K864004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: AmpR P1002
sequence annotation: VF2
sequence annotation: T3 terminator
sequence annotation: -35
sequence annotation: FRT site
sequence annotation: -10
sequence annotation: VR
sequence annotation: Lambda Red forward primer
sequence annotation: TonB terminator
sequence annotation: RBS
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: P22 late terminator
sequence annotation: rrnC terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: Lux terminator
sequence: https://synbiohub.org/public/igem/BBa_K864004_sequence/1

AmpR P1002
type: SequenceAnnotation
persistent identity: annotation2198875
display id: annotation2198875
version: 1
top level: BBa_K864004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198875/range2198875/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198875/range2198875/1
type: Range
persistent identity: range2198875
display id: range2198875
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 2543
end: 3403
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198873
display id: annotation2198873
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198873/range2198873/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198873/range2198873/1
type: Range
persistent identity: range2198873
display id: range2198873
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3564
end: 3583
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198874
display id: annotation2198874
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198874/range2198874/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198874/range2198874/1
type: Range
persistent identity: range2198874
display id: range2198874
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3584
end: 3661
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198878
display id: annotation2198878
version: 1
top level: BBa_K864004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198878/range2198878/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198878/range2198878/1
type: Range
persistent identity: range2198878
display id: range2198878
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3447
end: 3452
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2198880
display id: annotation2198880
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198880/range2198880/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198880/range2198880/1
type: Range
persistent identity: range2198880
display id: range2198880
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3483
end: 3516
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198877
display id: annotation2198877
version: 1
top level: BBa_K864004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198877/range2198877/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198877/range2198877/1
type: Range
persistent identity: range2198877
display id: range2198877
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3421
end: 3426
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198867
display id: annotation2198867
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198867/range2198867/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198867/range2198867/1
type: Range
persistent identity: range2198867
display id: range2198867
version: 1
direction: direction/forward
top level: BBa_K864004
start: 116
end: 135
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198870
display id: annotation2198870
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198870/range2198870/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198870/range2198870/1
type: Range
persistent identity: range2198870
display id: range2198870
version: 1
direction: direction/forward
top level: BBa_K864004
start: 2433
end: 2450
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2198866
display id: annotation2198866
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198866/range2198866/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198866/range2198866/1
type: Range
persistent identity: range2198866
display id: range2198866
version: 1
direction: direction/forward
top level: BBa_K864004
start: 47
end: 115
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2198876
display id: annotation2198876
version: 1
top level: BBa_K864004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198876/range2198876/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198876/range2198876/1
type: Range
persistent identity: range2198876
display id: range2198876
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3408
end: 3412
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2198869
display id: annotation2198869
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198869/range2198869/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198869/range2198869/1
type: Range
persistent identity: range2198869
display id: range2198869
version: 1
direction: direction/forward
top level: BBa_K864004
start: 190
end: 2405
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2198879
display id: annotation2198879
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198879/range2198879/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198879/range2198879/1
type: Range
persistent identity: range2198879
display id: range2198879
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 2506
end: 2539
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198872
display id: annotation2198872
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198872/range2198872/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198872/range2198872/1
type: Range
persistent identity: range2198872
display id: range2198872
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3523
end: 3563
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198868
display id: annotation2198868
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198868/range2198868/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198868/range2198868/1
type: Range
persistent identity: range2198868
display id: range2198868
version: 1
direction: direction/forward
top level: BBa_K864004
start: 136
end: 175
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198865
display id: annotation2198865
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198865/range2198865/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198865/range2198865/1
type: Range
persistent identity: range2198865
display id: range2198865
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 18
end: 38
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198871
display id: annotation2198871
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198871/range2198871/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198871/range2198871/1
type: Range
persistent identity: range2198871
display id: range2198871
version: 1
direction: direction/forward
top level: BBa_K864004
start: 2454
end: 2499
orientation: inline

pSB3C5
type: ComponentDefinition
persistent identity: pSB3C5
display id: pSB3C5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3C5 is a low to medium copy plasmid based with chloramphenicol resistance. It has a p15A-derived replication origin. pSB3C5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the chloramphenicol resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00102
component: BBa_B0062
component: BBa_B0053
component: BBa_G00001
component: BBa_G00100
component: BBa_G0002
component: BBa_G0004
component: BBa_B0044
component: BBa_P1004
component: BBa_B0054
component: BBa_I50032
component: BBa_G00000
component: BBa_G0003
component: BBa_B0042
component: BBa_B0043
component: BBa_B0055
sequence annotation: BBa_G0004
sequence annotation: BBa_B0054
sequence annotation: BBa_G00000
sequence annotation: BBa_B0055
sequence annotation: BBa_B0042
sequence annotation: BBa_B0062
sequence annotation: BBa_I50032
sequence annotation: BBa_G00001
sequence annotation: BBa_B0044
sequence annotation: BBa_G00100
sequence annotation: BBa_G00102
sequence annotation: BBa_B0053
sequence annotation: BBa_G0003
sequence annotation: BBa_G0002
sequence annotation: BBa_P1004
sequence annotation: BBa_B0043
sequence: https://synbiohub.org/public/igem/pSB3C5_sequence/1

BBa_G00102
type: Component
persistent identity: component1928326
display id: component1928326
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0062
type: Component
persistent identity: component1928328
display id: component1928328
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0053
type: Component
persistent identity: component1928338
display id: component1928338
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G00001
type: Component
persistent identity: component1928310
display id: component1928310
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00100
type: Component
persistent identity: component1928339
display id: component1928339
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G0002
type: Component
persistent identity: component1928333
display id: component1928333
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0004
type: Component
persistent identity: component1928336
display id: component1928336
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0044
type: Component
persistent identity: component1928315
display id: component1928315
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_P1004
type: Component
persistent identity: component1928335
display id: component1928335
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0054
type: Component
persistent identity: component1928325
display id: component1928325
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_I50032
type: Component
persistent identity: component1928332
display id: component1928332
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_G00000
type: Component
persistent identity: component1928356
display id: component1928356
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G0003
type: Component
persistent identity: component1928329
display id: component1928329
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0042
type: Component
persistent identity: component1928349
display id: component1928349
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component1928354
display id: component1928354
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component1928322
display id: component1928322
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0055
type: Component
persistent identity: component1928342
display id: component1928342
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1928336
display id: annotation1928336
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928336/range1928336/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3C5/annotation1928336/range1928336/1
type: Range
persistent identity: range1928336
display id: range1928336
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2515
end: 2521
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1928325
display id: annotation1928325
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928325/range1928325/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3C5/annotation1928325/range1928325/1
type: Range
persistent identity: range1928325
display id: range1928325
version: 1
direction: direction/reverse
top level: pSB3C5
start: 47
end: 115
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1928356
display id: annotation1928356
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928356/range1928356/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3C5/annotation1928356/range1928356/1
type: Range
persistent identity: range1928356
display id: range1928356
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2717
end: 2738
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1928342
display id: annotation1928342
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928342/range1928342/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3C5/annotation1928342/range1928342/1
type: Range
persistent identity: range1928342
display id: range1928342
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2614
end: 2691
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928349
display id: annotation1928349
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928349/range1928349/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3C5/annotation1928349/range1928349/1
type: Range
persistent identity: range1928349
display id: range1928349
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2692
end: 2703
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1928328
display id: annotation1928328
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928328/range1928328/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3C5/annotation1928328/range1928328/1
type: Range
persistent identity: range1928328
display id: range1928328
version: 1
direction: direction/reverse
top level: pSB3C5
start: 136
end: 176
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1928332
display id: annotation1928332
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928332/range1928332/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3C5/annotation1928332/range1928332/1
type: Range
persistent identity: range1928332
display id: range1928332
version: 1
direction: direction/reverse
top level: pSB3C5
start: 184
end: 1737
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1928310
display id: annotation1928310
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928310/range1928310/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3C5/annotation1928310/range1928310/1
type: Range
persistent identity: range1928310
display id: range1928310
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1
end: 21
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1928315
display id: annotation1928315
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928315/range1928315/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3C5/annotation1928315/range1928315/1
type: Range
persistent identity: range1928315
display id: range1928315
version: 1
direction: direction/reverse
top level: pSB3C5
start: 22
end: 34
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1928339
display id: annotation1928339
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928339/range1928339/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3C5/annotation1928339/range1928339/1
type: Range
persistent identity: range1928339
display id: range1928339
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2594
end: 2613
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1928326
display id: annotation1928326
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928326/range1928326/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3C5/annotation1928326/range1928326/1
type: Range
persistent identity: range1928326
display id: range1928326
version: 1
direction: direction/reverse
top level: pSB3C5
start: 116
end: 135
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1928338
display id: annotation1928338
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928338/range1928338/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3C5/annotation1928338/range1928338/1
type: Range
persistent identity: range1928338
display id: range1928338
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2522
end: 2593
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1928329
display id: annotation1928329
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928329/range1928329/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3C5/annotation1928329/range1928329/1
type: Range
persistent identity: range1928329
display id: range1928329
version: 1
direction: direction/reverse
top level: pSB3C5
start: 177
end: 183
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1928333
display id: annotation1928333
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928333/range1928333/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3C5/annotation1928333/range1928333/1
type: Range
persistent identity: range1928333
display id: range1928333
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1738
end: 1745
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928322
display id: annotation1928322
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928322/range1928322/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3C5/annotation1928322/range1928322/1
type: Range
persistent identity: range1928322
display id: range1928322
version: 1
direction: direction/reverse
top level: pSB3C5
start: 35
end: 46
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation1928335
display id: annotation1928335
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928335/range1928335/1
component: BBa_P1004

https://synbiohub.org/public/igem/pSB3C5/annotation1928335/range1928335/1
type: Range
persistent identity: range1928335
display id: range1928335
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1746
end: 2514
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1928354
display id: annotation1928354
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928354/range1928354/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3C5/annotation1928354/range1928354/1
type: Range
persistent identity: range1928354
display id: range1928354
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2704
end: 2716
orientation: inline

BBa_K864001
type: ComponentDefinition
persistent identity: BBa K864001
display id: BBa_K864001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4C15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR
sequence annotation: VF2
sequence annotation: Tandem repeat
sequence annotation: tonB terminator
sequence annotation: Promoter
sequence annotation: Lambda Red forward primer
sequence annotation: -35
sequence annotation: repA
sequence annotation: Mutated A>T to remove SpeI
sequence annotation: pSC101 ori
sequence annotation: Repeat "5"
sequence annotation: T3 terminator
sequence annotation: Repeat "4"
sequence annotation: CmR BBa_P1004
sequence annotation: Lux terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: -10
sequence annotation: rrnC terminator
sequence annotation: P22 late terminator
sequence: https://synbiohub.org/public/igem/BBa_K864001_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation2198565
display id: annotation2198565
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198565/range2198565/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198565/range2198565/1
type: Range
persistent identity: range2198565
display id: range2198565
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 116
end: 135
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198629
display id: annotation2198629
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198629/range2198629/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198629/range2198629/1
type: Range
persistent identity: range2198629
display id: range2198629
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3322
end: 3341
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198578
display id: annotation2198578
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198578/range2198578/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198578/range2198578/1
type: Range
persistent identity: range2198578
display id: range2198578
version: 1
direction: direction/forward
top level: BBa_K864001
start: 820
end: 841
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2198561
display id: annotation2198561
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198561/range2198561/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198561/range2198561/1
type: Range
persistent identity: range2198561
display id: range2198561
version: 1
direction: direction/forward
top level: BBa_K864001
start: 47
end: 115
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2198622
display id: annotation2198622
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198622/range2198622/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198622/range2198622/1
type: Range
persistent identity: range2198622
display id: range2198622
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 3235
end: 3261
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198606
display id: annotation2198606
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198606/range2198606/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198606/range2198606/1
type: Range
persistent identity: range2198606
display id: range2198606
version: 1
direction: direction/forward
top level: BBa_K864001
start: 2433
end: 2450
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198584
display id: annotation2198584
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198584/range2198584/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198584/range2198584/1
type: Range
persistent identity: range2198584
display id: range2198584
version: 1
direction: direction/forward
top level: BBa_K864001
start: 881
end: 886
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198574
display id: annotation2198574
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198574/range2198574/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198574/range2198574/1
type: Range
persistent identity: range2198574
display id: range2198574
version: 1
direction: direction/forward
top level: BBa_K864001
start: 767
end: 788
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2198605
display id: annotation2198605
version: 1
top level: BBa_K864001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198605/range2198605/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198605/range2198605/1
type: Range
persistent identity: range2198605
display id: range2198605
version: 1
direction: direction/forward
top level: BBa_K864001
start: 939
end: 1889
orientation: inline

Mutated A>T to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2198582
display id: annotation2198582
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198582/range2198582/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198582/range2198582/1
type: Range
persistent identity: range2198582
display id: range2198582
version: 1
direction: direction/forward
top level: BBa_K864001
start: 867
end: 867
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2198568
display id: annotation2198568
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198568/range2198568/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198568/range2198568/1
type: Range
persistent identity: range2198568
display id: range2198568
version: 1
direction: direction/forward
top level: BBa_K864001
start: 190
end: 2405
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2198585
display id: annotation2198585
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198585/range2198585/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198585/range2198585/1
type: Range
persistent identity: range2198585
display id: range2198585
version: 1
direction: direction/forward
top level: BBa_K864001
start: 891
end: 912
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198614
display id: annotation2198614
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198614/range2198614/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198614/range2198614/1
type: Range
persistent identity: range2198614
display id: range2198614
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3342
end: 3419
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2198580
display id: annotation2198580
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198580/range2198580/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198580/range2198580/1
type: Range
persistent identity: range2198580
display id: range2198580
version: 1
direction: direction/forward
top level: BBa_K864001
start: 862
end: 880
orientation: inline

CmR BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2198620
display id: annotation2198620
version: 1
top level: BBa_K864001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198620/range2198620/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198620/range2198620/1
type: Range
persistent identity: range2198620
display id: range2198620
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 2506
end: 3168
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198616
display id: annotation2198616
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198616/range2198616/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198616/range2198616/1
type: Range
persistent identity: range2198616
display id: range2198616
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 2454
end: 2499
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198340
display id: annotation2198340
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198340/range2198340/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198340/range2198340/1
type: Range
persistent identity: range2198340
display id: range2198340
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 18
end: 38
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198603
display id: annotation2198603
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198603/range2198603/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198603/range2198603/1
type: Range
persistent identity: range2198603
display id: range2198603
version: 1
direction: direction/forward
top level: BBa_K864001
start: 903
end: 908
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198576
display id: annotation2198576
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198576/range2198576/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198576/range2198576/1
type: Range
persistent identity: range2198576
display id: range2198576
version: 1
direction: direction/forward
top level: BBa_K864001
start: 790
end: 809
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198566
display id: annotation2198566
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198566/range2198566/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198566/range2198566/1
type: Range
persistent identity: range2198566
display id: range2198566
version: 1
direction: direction/forward
top level: BBa_K864001
start: 136
end: 175
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198628
display id: annotation2198628
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198628/range2198628/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198628/range2198628/1
type: Range
persistent identity: range2198628
display id: range2198628
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3251
end: 3321
orientation: inline

pSB6A1
type: ComponentDefinition
persistent identity: pSB6A1
display id: pSB6A1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBR322 converted to BioBrick vector (formally BBa_I739201)
creator: Stefan Luzi
created: 2007-10-22T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: sadf
mutable notes: asdf
mutable provenance: sdf
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: H-strand Y effector site
sequence annotation: beta-lactamase signal peptide
sequence annotation: L-strand Y effector site
sequence annotation: bla gene
sequence annotation: Repeat region 1
sequence annotation: RNAI transcript promoter -10 sequence
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: beta-lactamase (bla gene)
sequence annotation: BioBrick prefix
sequence annotation: bla(P3) promoter -35 sequence
sequence annotation: BioBrick suffix
sequence annotation: rep pMB1
sequence annotation: RNAII transcript
sequence annotation: bla(P3) promoter -10 sequence
sequence annotation: beta-lactamase mature peptide
sequence annotation: rop
sequence annotation: RNAI transcript promoter -35 sequence
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: gamma-delta insertion sequence
sequence annotation: from pSC101
sequence annotation: RNAI transcript
sequence annotation: partial tetracycline resistance CDS (nonfunctional)
sequence: https://synbiohub.org/public/igem/pSB6A1_sequence/1

H-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1969642
display id: annotation1969642
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969642/range1969642/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969642/range1969642/1
type: Range
persistent identity: range1969642
display id: range1969642
version: 1
direction: direction/forward
top level: pSB6A1
start: 1653
end: 1809
orientation: inline

beta-lactamase signal peptide
type: SequenceAnnotation
persistent identity: annotation1969652
display id: annotation1969652
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969652/range1969652/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969652/range1969652/1
type: Range
persistent identity: range1969652
display id: range1969652
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3727
end: 3795
orientation: inline

L-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1969643
display id: annotation1969643
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969643/range1969643/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969643/range1969643/1
type: Range
persistent identity: range1969643
display id: range1969643
version: 1
direction: direction/reverse
top level: pSB6A1
start: 1993
end: 2056
orientation: inline

bla gene
type: SequenceAnnotation
persistent identity: annotation1969654
display id: annotation1969654
version: 1
top level: pSB6A1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969654/range1969654/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969654/range1969654/1
type: Range
persistent identity: range1969654
display id: range1969654
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3803
end: 3807
orientation: inline

Repeat region 1
type: SequenceAnnotation
persistent identity: annotation1969657
display id: annotation1969657
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969657/range1969657/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969657/range1969657/1
type: Range
persistent identity: range1969657
display id: range1969657
version: 1
direction: direction/forward
top level: pSB6A1
start: 2790
end: 4003
orientation: inline

RNAI transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969649
display id: annotation1969649
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969649/range1969649/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969649/range1969649/1
type: Range
persistent identity: range1969649
display id: range1969649
version: 1
direction: direction/forward
top level: pSB6A1
start: 2604
end: 2609
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969651
display id: annotation1969651
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969651/range1969651/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969651/range1969651/1
type: Range
persistent identity: range1969651
display id: range1969651
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2759
end: 2764
orientation: inline

beta-lactamase (bla gene)
type: SequenceAnnotation
persistent identity: annotation1969647
display id: annotation1969647
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969647/range1969647/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969647/range1969647/1
type: Range
persistent identity: range1969647
display id: range1969647
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2935
end: 3795
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969658
display id: annotation1969658
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969658/range1969658/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969658/range1969658/1
type: Range
persistent identity: range1969658
display id: range1969658
version: 1
direction: direction/forward
top level: pSB6A1
start: 4001
end: 4022
orientation: inline

bla(P3) promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969656
display id: annotation1969656
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969656/range1969656/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969656/range1969656/1
type: Range
persistent identity: range1969656
display id: range1969656
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3860
end: 3865
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969637
display id: annotation1969637
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969637/range1969637/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969637/range1969637/1
type: Range
persistent identity: range1969637
display id: range1969637
version: 1
direction: direction/forward
top level: pSB6A1
start: 1
end: 21
orientation: inline

rep pMB1
type: SequenceAnnotation
persistent identity: annotation1969645
display id: annotation1969645
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969645/range1969645/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969645/range1969645/1
type: Range
persistent identity: range1969645
display id: range1969645
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2161
end: 2775
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1969644
display id: annotation1969644
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969644/range1969644/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969644/range1969644/1
type: Range
persistent identity: range1969644
display id: range1969644
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2176
end: 2728
orientation: inline

bla(P3) promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969655
display id: annotation1969655
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969655/range1969655/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969655/range1969655/1
type: Range
persistent identity: range1969655
display id: range1969655
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3839
end: 3844
orientation: inline

beta-lactamase mature peptide
type: SequenceAnnotation
persistent identity: annotation1969653
display id: annotation1969653
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969653/range1969653/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969653/range1969653/1
type: Range
persistent identity: range1969653
display id: range1969653
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2938
end: 3726
orientation: inline

rop
type: SequenceAnnotation
persistent identity: annotation1969641
display id: annotation1969641
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969641/range1969641/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969641/range1969641/1
type: Range
persistent identity: range1969641
display id: range1969641
version: 1
direction: direction/forward
top level: pSB6A1
start: 1557
end: 1748
orientation: inline

RNAI transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969648
display id: annotation1969648
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969648/range1969648/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969648/range1969648/1
type: Range
persistent identity: range1969648
display id: range1969648
version: 1
direction: direction/forward
top level: pSB6A1
start: 2582
end: 2587
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969650
display id: annotation1969650
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969650/range1969650/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969650/range1969650/1
type: Range
persistent identity: range1969650
display id: range1969650
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2738
end: 2743
orientation: inline

gamma-delta insertion sequence
type: SequenceAnnotation
persistent identity: annotation1969639
display id: annotation1969639
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969639/range1969639/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969639/range1969639/1
type: Range
persistent identity: range1969639
display id: range1969639
version: 1
direction: direction/reverse
top level: pSB6A1
start: 1157
end: 1161
orientation: inline

from pSC101
type: SequenceAnnotation
persistent identity: annotation1969640
display id: annotation1969640
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969640/range1969640/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969640/range1969640/1
type: Range
persistent identity: range1969640
display id: range1969640
version: 1
direction: direction/forward
top level: pSB6A1
start: 1278
end: 1404
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1969646
display id: annotation1969646
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969646/range1969646/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969646/range1969646/1
type: Range
persistent identity: range1969646
display id: range1969646
version: 1
direction: direction/forward
top level: pSB6A1
start: 2618
end: 2725
orientation: inline

partial tetracycline resistance CDS (nonfunctional)
type: SequenceAnnotation
persistent identity: annotation1969638
display id: annotation1969638
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969638/range1969638/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969638/range1969638/1
type: Range
persistent identity: range1969638
display id: range1969638
version: 1
direction: direction/forward
top level: pSB6A1
start: 23
end: 918
orientation: inline

pSB4C50
type: ComponentDefinition
persistent identity: BBa K1362096
display id: BBa_K1362096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4C50: Low(?) copy BioBrick cloning/expression backbone carrying Cm resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00001
component: BBa_G0004
component: BBa_I50042
component: BBa_B0043
component: BBa_B0053
component: BBa_B0055
component: BBa_B0042
component: BBa_G00000
component: BBa_B0044
component: BBa_B0062
component: BBa_G0002
component: BBa_G0003
component: BBa_G00100
component: BBa_B0054
component: BBa_G00102
component: BBa_P1004
component: BBa_K1362463
sequence annotation: BBa_G00001
sequence annotation: BBa_B0043
sequence annotation: BBa_G00000
sequence annotation: BBa_B0062
sequence annotation: BBa_B0053
sequence annotation: BBa_G0004
sequence annotation: BBa_I50042
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_P1004
sequence annotation: BBa_G0003
sequence annotation: BBa_B0044
sequence annotation: BBa_B0055
sequence annotation: BBa_G0002
sequence annotation: BBa_K1362463
sequence annotation: BBa_G00100
sequence annotation: BBa_G00102
sequence: https://synbiohub.org/public/igem/BBa_K1362096_sequence/1

BBa_G00001
type: Component
persistent identity: component2409852
display id: component2409852
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G0004
type: Component
persistent identity: component2409904
display id: component2409904
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_I50042
type: Component
persistent identity: component2409900
display id: component2409900
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0043
type: Component
persistent identity: component2409923
display id: component2409923
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0053
type: Component
persistent identity: component2409906
display id: component2409906
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0055
type: Component
persistent identity: component2409911
display id: component2409911
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component2409918
display id: component2409918
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00000
type: Component
persistent identity: component2409932
display id: component2409932
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0042
type: Component
persistent identity: component2409864
display id: component2409864
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component2409857
display id: component2409857
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409871
display id: component2409871
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0002
type: Component
persistent identity: component2409901
display id: component2409901
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0003
type: Component
persistent identity: component2409872
display id: component2409872
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00100
type: Component
persistent identity: component2409908
display id: component2409908
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0054
type: Component
persistent identity: component2409867
display id: component2409867
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00102
type: Component
persistent identity: component2409869
display id: component2409869
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_P1004
type: Component
persistent identity: component2409903
display id: component2409903
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_K1362463
type: Component
persistent identity: component2409927
display id: component2409927
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409852
display id: annotation2409852
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
type: Range
persistent identity: range2409852
display id: range2409852
version: 1
direction: direction/reverse
top level: pSB4C50
start: 1
end: 21
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409923
display id: annotation2409923
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
type: Range
persistent identity: range2409923
display id: range2409923
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3307
end: 3319
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409932
display id: annotation2409932
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
type: Range
persistent identity: range2409932
display id: range2409932
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3387
end: 3408
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409871
display id: annotation2409871
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
type: Range
persistent identity: range2409871
display id: range2409871
version: 1
direction: direction/reverse
top level: pSB4C50
start: 176
end: 216
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409906
display id: annotation2409906
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
type: Range
persistent identity: range2409906
display id: range2409906
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3093
end: 3164
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409904
display id: annotation2409904
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
type: Range
persistent identity: range2409904
display id: range2409904
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3078
end: 3084
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409900
display id: annotation2409900
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
type: Range
persistent identity: range2409900
display id: range2409900
version: 1
direction: direction/reverse
top level: pSB4C50
start: 240
end: 2276
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409918
display id: annotation2409918
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
type: Range
persistent identity: range2409918
display id: range2409918
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3287
end: 3298
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409864
display id: annotation2409864
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
type: Range
persistent identity: range2409864
display id: range2409864
version: 1
direction: direction/reverse
top level: pSB4C50
start: 51
end: 62
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409867
display id: annotation2409867
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
type: Range
persistent identity: range2409867
display id: range2409867
version: 1
direction: direction/reverse
top level: pSB4C50
start: 71
end: 139
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2409903
display id: annotation2409903
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
component: BBa_P1004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
type: Range
persistent identity: range2409903
display id: range2409903
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2301
end: 3069
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409872
display id: annotation2409872
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
type: Range
persistent identity: range2409872
display id: range2409872
version: 1
direction: direction/reverse
top level: pSB4C50
start: 225
end: 231
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409857
display id: annotation2409857
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
type: Range
persistent identity: range2409857
display id: range2409857
version: 1
direction: direction/reverse
top level: pSB4C50
start: 30
end: 42
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409911
display id: annotation2409911
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
type: Range
persistent identity: range2409911
display id: range2409911
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3201
end: 3278
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409901
display id: annotation2409901
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
type: Range
persistent identity: range2409901
display id: range2409901
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2285
end: 2292
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409927
display id: annotation2409927
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
type: Range
persistent identity: range2409927
display id: range2409927
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3328
end: 3378
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409908
display id: annotation2409908
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
type: Range
persistent identity: range2409908
display id: range2409908
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3173
end: 3192
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409869
display id: annotation2409869
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
type: Range
persistent identity: range2409869
display id: range2409869
version: 1
direction: direction/reverse
top level: pSB4C50
start: 148
end: 167
orientation: inline

pSB4A5
type: ComponentDefinition
persistent identity: pSB4A5
display id: pSB4A5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-26T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4A5 is a low copy plasmid with ampicillin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
mutable provenance: BBa_I51001, pSC101 origin and a new version of ampR stripped of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0054
component: BBa_B0042
component: BBa_G00100
component: BBa_G00102
component: BBa_B0043
component: BBa_I50042
component: BBa_B0044
component: BBa_B0053
component: BBa_G0004
component: BBa_G00000
component: BBa_G00001
component: BBa_G0002
component: BBa_P1002
component: BBa_B0055
component: BBa_G0003
component: BBa_B0062
sequence annotation: BBa_G00102
sequence annotation: BBa_B0054
sequence annotation: BBa_G0002
sequence annotation: BBa_B0042
sequence annotation: BBa_B0055
sequence annotation: BBa_G00001
sequence annotation: BBa_B0062
sequence annotation: BBa_P1002
sequence annotation: BBa_B0043
sequence annotation: BBa_B0044
sequence annotation: BBa_G00000
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence annotation: BBa_G0003
sequence annotation: BBa_I50042
sequence annotation: BBa_G0004
sequence: https://synbiohub.org/public/igem/pSB4A5_sequence/1

BBa_B0054
type: Component
persistent identity: component2236332
display id: component2236332
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0042
type: Component
persistent identity: component2236329
display id: component2236329
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00100
type: Component
persistent identity: component2236376
display id: component2236376
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00102
type: Component
persistent identity: component2236334
display id: component2236334
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0043
type: Component
persistent identity: component2236391
display id: component2236391
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_I50042
type: Component
persistent identity: component2236365
display id: component2236365
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0044
type: Component
persistent identity: component2236322
display id: component2236322
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0053
type: Component
persistent identity: component2236374
display id: component2236374
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0042
type: Component
persistent identity: component2236386
display id: component2236386
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0004
type: Component
persistent identity: component2236372
display id: component2236372
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00000
type: Component
persistent identity: component2236396
display id: component2236396
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00001
type: Component
persistent identity: component2236317
display id: component2236317
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G0002
type: Component
persistent identity: component2236366
display id: component2236366
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_P1002
type: Component
persistent identity: component2236371
display id: component2236371
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_B0055
type: Component
persistent identity: component2236379
display id: component2236379
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G0003
type: Component
persistent identity: component2236337
display id: component2236337
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0062
type: Component
persistent identity: component2236336
display id: component2236336
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236334
display id: annotation2236334
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236334/range2236334/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4A5/annotation2236334/range2236334/1
type: Range
persistent identity: range2236334
display id: range2236334
version: 1
direction: direction/reverse
top level: pSB4A5
start: 116
end: 135
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236332
display id: annotation2236332
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236332/range2236332/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4A5/annotation2236332/range2236332/1
type: Range
persistent identity: range2236332
display id: range2236332
version: 1
direction: direction/reverse
top level: pSB4A5
start: 47
end: 115
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236366
display id: annotation2236366
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236366/range2236366/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4A5/annotation2236366/range2236366/1
type: Range
persistent identity: range2236366
display id: range2236366
version: 1
direction: direction/reverse
top level: pSB4A5
start: 2221
end: 2228
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236386
display id: annotation2236386
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236386/range2236386/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4A5/annotation2236386/range2236386/1
type: Range
persistent identity: range2236386
display id: range2236386
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3349
end: 3360
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236379
display id: annotation2236379
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236379/range2236379/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4A5/annotation2236379/range2236379/1
type: Range
persistent identity: range2236379
display id: range2236379
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3271
end: 3348
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236317
display id: annotation2236317
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236317/range2236317/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4A5/annotation2236317/range2236317/1
type: Range
persistent identity: range2236317
display id: range2236317
version: 1
direction: direction/reverse
top level: pSB4A5
start: 1
end: 21
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236336
display id: annotation2236336
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236336/range2236336/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4A5/annotation2236336/range2236336/1
type: Range
persistent identity: range2236336
display id: range2236336
version: 1
direction: direction/reverse
top level: pSB4A5
start: 136
end: 176
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2236371
display id: annotation2236371
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236371/range2236371/1
component: BBa_P1002

https://synbiohub.org/public/igem/pSB4A5/annotation2236371/range2236371/1
type: Range
persistent identity: range2236371
display id: range2236371
version: 1
direction: direction/reverse
top level: pSB4A5
start: 2229
end: 3171
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236391
display id: annotation2236391
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236391/range2236391/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4A5/annotation2236391/range2236391/1
type: Range
persistent identity: range2236391
display id: range2236391
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3361
end: 3373
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236322
display id: annotation2236322
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236322/range2236322/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4A5/annotation2236322/range2236322/1
type: Range
persistent identity: range2236322
display id: range2236322
version: 1
direction: direction/reverse
top level: pSB4A5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236329
display id: annotation2236329
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236329/range2236329/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4A5/annotation2236329/range2236329/1
type: Range
persistent identity: range2236329
display id: range2236329
version: 1
direction: direction/reverse
top level: pSB4A5
start: 35
end: 46
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236396
display id: annotation2236396
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236396/range2236396/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4A5/annotation2236396/range2236396/1
type: Range
persistent identity: range2236396
display id: range2236396
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3374
end: 3395
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236374
display id: annotation2236374
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236374/range2236374/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4A5/annotation2236374/range2236374/1
type: Range
persistent identity: range2236374
display id: range2236374
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3179
end: 3250
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236376
display id: annotation2236376
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236376/range2236376/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4A5/annotation2236376/range2236376/1
type: Range
persistent identity: range2236376
display id: range2236376
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3251
end: 3270
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236337
display id: annotation2236337
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236337/range2236337/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4A5/annotation2236337/range2236337/1
type: Range
persistent identity: range2236337
display id: range2236337
version: 1
direction: direction/reverse
top level: pSB4A5
start: 177
end: 183
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236365
display id: annotation2236365
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236365/range2236365/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4A5/annotation2236365/range2236365/1
type: Range
persistent identity: range2236365
display id: range2236365
version: 1
direction: direction/reverse
top level: pSB4A5
start: 184
end: 2220
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236372
display id: annotation2236372
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236372/range2236372/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4A5/annotation2236372/range2236372/1
type: Range
persistent identity: range2236372
display id: range2236372
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3172
end: 3178
orientation: inline

BBa_K864000
type: ComponentDefinition
persistent identity: BBa K864000
display id: BBa_K864000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ??? antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Repeat "5"
sequence annotation: pSC101 ori
sequence annotation: tonB terminator
sequence annotation: Mutated A>T to remove SpeI
sequence annotation: T3 terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: Tandem repeat
sequence annotation: rrnC terminator
sequence annotation: Lambda Red forward primer
sequence annotation: -10
sequence annotation: Repeat "4"
sequence annotation: VR
sequence annotation: ampR BBa_P1002
sequence annotation: repA
sequence annotation: Lux terminator
sequence annotation: RBS
sequence annotation: VF2
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K864000_sequence/1

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2198056
display id: annotation2198056
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198056/range2198056/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198056/range2198056/1
type: Range
persistent identity: range2198056
display id: range2198056
version: 1
direction: direction/forward
top level: BBa_K864000
start: 891
end: 912
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2198057
display id: annotation2198057
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198057/range2198057/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198057/range2198057/1
type: Range
persistent identity: range2198057
display id: range2198057
version: 1
direction: direction/forward
top level: BBa_K864000
start: 190
end: 2405
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2198074
display id: annotation2198074
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198074/range2198074/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198074/range2198074/1
type: Range
persistent identity: range2198074
display id: range2198074
version: 1
direction: direction/forward
top level: BBa_K864000
start: 47
end: 115
orientation: inline

Mutated A>T to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2198029
display id: annotation2198029
version: 1
top level: BBa_K864000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198029/range2198029/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198029/range2198029/1
type: Range
persistent identity: range2198029
display id: range2198029
version: 1
direction: direction/forward
top level: BBa_K864000
start: 867
end: 867
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198076
display id: annotation2198076
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198076/range2198076/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198076/range2198076/1
type: Range
persistent identity: range2198076
display id: range2198076
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3515
end: 3592
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198031
display id: annotation2198031
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198031/range2198031/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198031/range2198031/1
type: Range
persistent identity: range2198031
display id: range2198031
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 18
end: 38
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198037
display id: annotation2198037
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198037/range2198037/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198037/range2198037/1
type: Range
persistent identity: range2198037
display id: range2198037
version: 1
direction: direction/forward
top level: BBa_K864000
start: 767
end: 788
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198093
display id: annotation2198093
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198093/range2198093/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198093/range2198093/1
type: Range
persistent identity: range2198093
display id: range2198093
version: 1
direction: direction/forward
top level: BBa_K864000
start: 136
end: 175
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198030
display id: annotation2198030
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198030/range2198030/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198030/range2198030/1
type: Range
persistent identity: range2198030
display id: range2198030
version: 1
direction: direction/forward
top level: BBa_K864000
start: 2433
end: 2450
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198007
display id: annotation2198007
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198007/range2198007/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198007/range2198007/1
type: Range
persistent identity: range2198007
display id: range2198007
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 3387
end: 3392
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2198038
display id: annotation2198038
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198038/range2198038/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198038/range2198038/1
type: Range
persistent identity: range2198038
display id: range2198038
version: 1
direction: direction/forward
top level: BBa_K864000
start: 862
end: 880
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198039
display id: annotation2198039
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198039/range2198039/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198039/range2198039/1
type: Range
persistent identity: range2198039
display id: range2198039
version: 1
direction: direction/forward
top level: BBa_K864000
start: 820
end: 841
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198033
display id: annotation2198033
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198033/range2198033/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198033/range2198033/1
type: Range
persistent identity: range2198033
display id: range2198033
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 116
end: 135
orientation: inline

ampR BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2198012
display id: annotation2198012
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198012/range2198012/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198012/range2198012/1
type: Range
persistent identity: range2198012
display id: range2198012
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 2509
end: 3369
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2198011
display id: annotation2198011
version: 1
top level: BBa_K864000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198011/range2198011/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198011/range2198011/1
type: Range
persistent identity: range2198011
display id: range2198011
version: 1
direction: direction/forward
top level: BBa_K864000
start: 939
end: 1889
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198006
display id: annotation2198006
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198006/range2198006/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198006/range2198006/1
type: Range
persistent identity: range2198006
display id: range2198006
version: 1
direction: direction/forward
top level: BBa_K864000
start: 903
end: 908
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198036
display id: annotation2198036
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198036/range2198036/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198036/range2198036/1
type: Range
persistent identity: range2198036
display id: range2198036
version: 1
direction: direction/forward
top level: BBa_K864000
start: 790
end: 809
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198075
display id: annotation2198075
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198075/range2198075/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198075/range2198075/1
type: Range
persistent identity: range2198075
display id: range2198075
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 2454
end: 2499
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2198034
display id: annotation2198034
version: 1
top level: BBa_K864000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198034/range2198034/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198034/range2198034/1
type: Range
persistent identity: range2198034
display id: range2198034
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3374
end: 3378
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198032
display id: annotation2198032
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198032/range2198032/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198032/range2198032/1
type: Range
persistent identity: range2198032
display id: range2198032
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3495
end: 3514
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198009
display id: annotation2198009
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198009/range2198009/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198009/range2198009/1
type: Range
persistent identity: range2198009
display id: range2198009
version: 1
direction: direction/forward
top level: BBa_K864000
start: 881
end: 886
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198010
display id: annotation2198010
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198010/range2198010/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198010/range2198010/1
type: Range
persistent identity: range2198010
display id: range2198010
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 3413
end: 3418
orientation: inline

pSB4K5
type: ComponentDefinition
persistent identity: pSB4K5
display id: pSB4K5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4K5 is a low copy plasmid with kanamycin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design for more information.
mutable provenance: BBa_I51001 vector scaffold, BBa_I50040 replication origin and BBa_P1003 antibiotic resistance marker.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G0006
component: BBa_B0062
component: BBa_G00100
component: BBa_B0054
component: BBa_G0001
component: BBa_B0055
component: BBa_G0005
component: BBa_G00001
component: BBa_I50041
component: BBa_B0044
component: BBa_B0042
component: BBa_B0053
component: BBa_P1007
component: BBa_G00000
component: BBa_G00102
component: BBa_B0043
sequence annotation: BBa_B0053
sequence annotation: BBa_B0062
sequence annotation: BBa_G00001
sequence annotation: BBa_G00000
sequence annotation: BBa_I50041
sequence annotation: BBa_P1007
sequence annotation: BBa_B0042
sequence annotation: BBa_B0055
sequence annotation: BBa_G00100
sequence annotation: BBa_B0054
sequence annotation: BBa_B0044
sequence annotation: BBa_B0043
sequence annotation: BBa_G00102
sequence annotation: BBa_G0001
sequence annotation: BBa_G0005
sequence annotation: BBa_G0006
sequence: https://synbiohub.org/public/igem/pSB4K5_sequence/1

BBa_G0006
type: Component
persistent identity: component2236031
display id: component2236031
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0006/1

BBa_B0062
type: Component
persistent identity: component2235998
display id: component2235998
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G00100
type: Component
persistent identity: component2236035
display id: component2236035
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0054
type: Component
persistent identity: component2235994
display id: component2235994
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G0001
type: Component
persistent identity: component2236004
display id: component2236004
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0001/1

BBa_B0055
type: Component
persistent identity: component2236038
display id: component2236038
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G0005
type: Component
persistent identity: component2235999
display id: component2235999
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0005/1

BBa_G00001
type: Component
persistent identity: component2235979
display id: component2235979
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_I50041
type: Component
persistent identity: component2236030
display id: component2236030
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50041/1

BBa_B0044
type: Component
persistent identity: component2235984
display id: component2235984
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0042
type: Component
persistent identity: component2235991
display id: component2235991
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0053
type: Component
persistent identity: component2236033
display id: component2236033
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_P1007
type: Component
persistent identity: component2236003
display id: component2236003
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1007/1

BBa_G00000
type: Component
persistent identity: component2236055
display id: component2236055
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00102
type: Component
persistent identity: component2235996
display id: component2235996
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component2236045
display id: component2236045
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component2236050
display id: component2236050
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236033
display id: annotation2236033
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236033/range2236033/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4K5/annotation2236033/range2236033/1
type: Range
persistent identity: range2236033
display id: range2236033
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3203
end: 3274
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2235998
display id: annotation2235998
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235998/range2235998/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4K5/annotation2235998/range2235998/1
type: Range
persistent identity: range2235998
display id: range2235998
version: 1
direction: direction/reverse
top level: pSB4K5
start: 136
end: 176
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2235979
display id: annotation2235979
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235979/range2235979/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4K5/annotation2235979/range2235979/1
type: Range
persistent identity: range2235979
display id: range2235979
version: 1
direction: direction/reverse
top level: pSB4K5
start: 1
end: 21
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236055
display id: annotation2236055
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236055/range2236055/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4K5/annotation2236055/range2236055/1
type: Range
persistent identity: range2236055
display id: range2236055
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3398
end: 3419
orientation: inline

BBa_I50041
type: SequenceAnnotation
persistent identity: annotation2236030
display id: annotation2236030
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236030/range2236030/1
component: BBa_I50041

https://synbiohub.org/public/igem/pSB4K5/annotation2236030/range2236030/1
type: Range
persistent identity: range2236030
display id: range2236030
version: 1
direction: direction/reverse
top level: pSB4K5
start: 1159
end: 3195
orientation: inline

BBa_P1007
type: SequenceAnnotation
persistent identity: annotation2236003
display id: annotation2236003
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236003/range2236003/1
component: BBa_P1007

https://synbiohub.org/public/igem/pSB4K5/annotation2236003/range2236003/1
type: Range
persistent identity: range2236003
display id: range2236003
version: 1
direction: direction/reverse
top level: pSB4K5
start: 184
end: 1150
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2235991
display id: annotation2235991
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235991/range2235991/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4K5/annotation2235991/range2235991/1
type: Range
persistent identity: range2235991
display id: range2235991
version: 1
direction: direction/reverse
top level: pSB4K5
start: 35
end: 46
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236045
display id: annotation2236045
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236045/range2236045/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4K5/annotation2236045/range2236045/1
type: Range
persistent identity: range2236045
display id: range2236045
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3373
end: 3384
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236038
display id: annotation2236038
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236038/range2236038/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4K5/annotation2236038/range2236038/1
type: Range
persistent identity: range2236038
display id: range2236038
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3295
end: 3372
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236035
display id: annotation2236035
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236035/range2236035/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4K5/annotation2236035/range2236035/1
type: Range
persistent identity: range2236035
display id: range2236035
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3275
end: 3294
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2235994
display id: annotation2235994
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235994/range2235994/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4K5/annotation2235994/range2235994/1
type: Range
persistent identity: range2235994
display id: range2235994
version: 1
direction: direction/reverse
top level: pSB4K5
start: 47
end: 115
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2235984
display id: annotation2235984
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235984/range2235984/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4K5/annotation2235984/range2235984/1
type: Range
persistent identity: range2235984
display id: range2235984
version: 1
direction: direction/reverse
top level: pSB4K5
start: 22
end: 34
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236050
display id: annotation2236050
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236050/range2236050/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4K5/annotation2236050/range2236050/1
type: Range
persistent identity: range2236050
display id: range2236050
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3385
end: 3397
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2235996
display id: annotation2235996
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235996/range2235996/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4K5/annotation2235996/range2235996/1
type: Range
persistent identity: range2235996
display id: range2235996
version: 1
direction: direction/reverse
top level: pSB4K5
start: 116
end: 135
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2236004
display id: annotation2236004
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236004/range2236004/1
component: BBa_G0001

https://synbiohub.org/public/igem/pSB4K5/annotation2236004/range2236004/1
type: Range
persistent identity: range2236004
display id: range2236004
version: 1
direction: direction/reverse
top level: pSB4K5
start: 1151
end: 1158
orientation: inline

BBa_G0005
type: SequenceAnnotation
persistent identity: annotation2235999
display id: annotation2235999
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235999/range2235999/1
component: BBa_G0005

https://synbiohub.org/public/igem/pSB4K5/annotation2235999/range2235999/1
type: Range
persistent identity: range2235999
display id: range2235999
version: 1
direction: direction/reverse
top level: pSB4K5
start: 177
end: 183
orientation: inline

BBa_G0006
type: SequenceAnnotation
persistent identity: annotation2236031
display id: annotation2236031
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236031/range2236031/1
component: BBa_G0006

https://synbiohub.org/public/igem/pSB4K5/annotation2236031/range2236031/1
type: Range
persistent identity: range2236031
display id: range2236031
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3196
end: 3202
orientation: inline

pSB8K15
type: ComponentDefinition
persistent identity: BBa K864018
display id: BBa_K864018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8K15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864018_sequence/1

pSB1AC3
type: ComponentDefinition
persistent identity: pSB1AC3
display id: pSB1AC3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Tom Knight
created: 2005-06-06T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _42_
m user id: 0
owner id: 6
owning group id: 42
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.
mutable provenance: pSB1A3 with added Cm resistance
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VF2
sequence annotation: Silent g->a mutation found during sequencing
sequence annotation: Prefix
sequence annotation: E. coli his operon terminator
sequence annotation: Suffix
sequence annotation: Cm R (rev)
sequence annotation: deletion of two bases
sequence annotation: terminator stem loop
sequence annotation: APr (rev)
sequence annotation: ORI
sequence annotation: rep (pMB1) (rev)
sequence annotation: end of terminator
sequence annotation: VR
sequence annotation: T0 terminator
sequence: https://synbiohub.org/public/igem/pSB1AC3_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1779730
display id: annotation1779730
version: 1
top level: pSB1AC3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779730/range1779730/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779730/range1779730/1
type: Range
persistent identity: range1779730
display id: range1779730
version: 1
direction: direction/forward
top level: pSB1AC3
start: 2916
end: 2935
orientation: inline

Silent g->a mutation found during sequencing
type: SequenceAnnotation
persistent identity: annotation1782173
display id: annotation1782173
version: 1
top level: pSB1AC3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1782173/range1782173/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1782173/range1782173/1
type: Range
persistent identity: range1782173
display id: range1782173
version: 1
direction: direction/forward
top level: pSB1AC3
start: 1237
end: 1237
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1779618
display id: annotation1779618
version: 1
top level: pSB1AC3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779618/range1779618/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779618/range1779618/1
type: Range
persistent identity: range1779618
display id: range1779618
version: 1
direction: direction/forward
top level: pSB1AC3
start: 3034
end: 3055
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1823948
display id: annotation1823948
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1823948/range1823948/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1823948/range1823948/1
type: Range
persistent identity: range1823948
display id: range1823948
version: 1
direction: direction/forward
top level: pSB1AC3
start: 22
end: 93
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1779617
display id: annotation1779617
version: 1
top level: pSB1AC3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779617/range1779617/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779617/range1779617/1
type: Range
persistent identity: range1779617
display id: range1779617
version: 1
direction: direction/forward
top level: pSB1AC3
start: 1
end: 21
orientation: inline

Cm R (rev)
type: SequenceAnnotation
persistent identity: annotation1779657
display id: annotation1779657
version: 1
top level: pSB1AC3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779657/range1779657/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779657/range1779657/1
type: Range
persistent identity: range1779657
display id: range1779657
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 1163
end: 1821
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823815
display id: annotation1823815
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1823815/range1823815/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1823815/range1823815/1
type: Range
persistent identity: range1823815
display id: range1823815
version: 1
direction: direction/forward
top level: pSB1AC3
start: 34
end: 35
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1779720
display id: annotation1779720
version: 1
top level: pSB1AC3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779720/range1779720/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779720/range1779720/1
type: Range
persistent identity: range1779720
display id: range1779720
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 3005
end: 3024
orientation: inline

APr (rev)
type: SequenceAnnotation
persistent identity: annotation1779661
display id: annotation1779661
version: 1
top level: pSB1AC3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779661/range1779661/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779661/range1779661/1
type: Range
persistent identity: range1779661
display id: range1779661
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 1933
end: 2793
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1779653
display id: annotation1779653
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779653/range1779653/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779653/range1779653/1
type: Range
persistent identity: range1779653
display id: range1779653
version: 1
direction: direction/forward
top level: pSB1AC3
start: 276
end: 276
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1779628
display id: annotation1779628
version: 1
top level: pSB1AC3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779628/range1779628/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779628/range1779628/1
type: Range
persistent identity: range1779628
display id: range1779628
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 261
end: 875
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1823949
display id: annotation1823949
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1823949/range1823949/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1823949/range1823949/1
type: Range
persistent identity: range1823949
display id: range1823949
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 2992
end: 2995
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1779665
display id: annotation1779665
version: 1
top level: pSB1AC3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779665/range1779665/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779665/range1779665/1
type: Range
persistent identity: range1779665
display id: range1779665
version: 1
direction: direction/forward
top level: pSB1AC3
start: 30
end: 64
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1779731
display id: annotation1779731
version: 1
top level: pSB1AC3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779731/range1779731/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779731/range1779731/1
type: Range
persistent identity: range1779731
display id: range1779731
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 157
end: 176
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation1782172
display id: annotation1782172
version: 1
top level: pSB1AC3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1782172/range1782172/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1782172/range1782172/1
type: Range
persistent identity: range1782172
display id: range1782172
version: 1
direction: direction/forward
top level: pSB1AC3
start: 1042
end: 1147
orientation: inline

BBa_K864005
type: ComponentDefinition
persistent identity: BBa K864005
display id: BBa_K864005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. The resistance cassette is flanked by FRT sites (BBa_J61020) which allows for subsequent removal of the resistance cassette from the chromosome by the FLP recombinase. The pSB8x15 series are especially suitable for carrying the FLP recombinase, since they can be removed by incubation at 42??C.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Lambda Red forward primer
sequence annotation: Lambda Red reverse primer
sequence annotation: VR
sequence annotation: TonB terminator
sequence annotation: Lux terminator
sequence annotation: rrnC terminator
sequence annotation: pSC101 ori BBa_K864050
sequence: https://synbiohub.org/public/igem/BBa_K864005_sequence/1

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2200050
display id: annotation2200050
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200050/range2200050/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200050/range2200050/1
type: Range
persistent identity: range2200050
display id: range2200050
version: 1
direction: direction/forward
top level: BBa_K864005
start: 2433
end: 2450
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2200041
display id: annotation2200041
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200041/range2200041/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200041/range2200041/1
type: Range
persistent identity: range2200041
display id: range2200041
version: 1
direction: direction/reverse
top level: BBa_K864005
start: 18
end: 38
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2200046
display id: annotation2200046
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200046/range2200046/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200046/range2200046/1
type: Range
persistent identity: range2200046
display id: range2200046
version: 1
direction: direction/reverse
top level: BBa_K864005
start: 116
end: 135
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2200045
display id: annotation2200045
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200045/range2200045/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200045/range2200045/1
type: Range
persistent identity: range2200045
display id: range2200045
version: 1
direction: direction/forward
top level: BBa_K864005
start: 47
end: 115
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2200051
display id: annotation2200051
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200051/range2200051/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200051/range2200051/1
type: Range
persistent identity: range2200051
display id: range2200051
version: 1
direction: direction/forward
top level: BBa_K864005
start: 2454
end: 2499
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2200047
display id: annotation2200047
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200047/range2200047/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200047/range2200047/1
type: Range
persistent identity: range2200047
display id: range2200047
version: 1
direction: direction/forward
top level: BBa_K864005
start: 136
end: 175
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2200048
display id: annotation2200048
version: 1
top level: BBa_K864005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200048/range2200048/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200048/range2200048/1
type: Range
persistent identity: range2200048
display id: range2200048
version: 1
direction: direction/forward
top level: BBa_K864005
start: 190
end: 2405
orientation: inline