//plasmidbackbone/component/selectionmarker/antibioticresistance
type: Collection
persistent identity: plasmidbackbone component selectionmarker antibioticresistance
display id: plasmidbackbone_component_selectionmarker_antibioticresistance
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: gmR
member: BBa_K292003
member: cmR
member: ampR
member: ble
member: BBa_K1937002
member: BBa_K292004
member: GmR
member: PcpcB-Kana
member: tetA
member: kanR
member: CmR
member: TetR

//plasmidbackbone/component/selectionmarker
type: Collection
persistent identity: plasmidbackbone component selectionmarker
display id: plasmidbackbone_component_selectionmarker
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //plasmidbackbone/component/selectionmarker/antibioticresistance

//plasmidbackbone/component/defaultinsert
type: Collection
persistent identity: plasmidbackbone component defaultinsert
display id: plasmidbackbone_component_defaultinsert
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: OnRFP
member: Default in
member: BBa_I52001
member: ccdB
member: AD default
member: BBa_I52002

//plasmidbackbone/component
type: Collection
persistent identity: plasmidbackbone component
display id: plasmidbackbone_component
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //plasmidbackbone/component/selectionmarker
member: //plasmidbackbone/component/defaultinsert

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa P1016
display id: BBa_P1016
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB cassette without ccdA-
created: 2007-01-29T12:00:00Z
modified: 2015-06-15T01:13:47Z
mutable provenance: This part is derived from BBa_P1010 but it lacks ccdA-.
owned by: james
owned by: myers
mutable description: This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 4206
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: -35
sequence annotation: -10
sequence annotation: ccdB
sequence: https://synbiohub.org/public/igem/BBa_P1016_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1910684
display id: annotation1910684
version: 1
top level: ccdB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1

https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
type: Range
persistent identity: range1910684
display id: range1910684
version: 1
top level: ccdB
direction: direction/forward
start: 41
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1910685
display id: annotation1910685
version: 1
top level: ccdB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1

https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
type: Range
persistent identity: range1910685
display id: range1910685
version: 1
top level: ccdB
direction: direction/forward
start: 64
end: 69
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation1910686
display id: annotation1910686
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1

https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
type: Range
persistent identity: range1910686
display id: range1910686
version: 1
top level: ccdB
direction: direction/forward
start: 108
end: 416
orientation: inline

gmR
type: ComponentDefinition
persistent identity: BBa P1013
display id: BBa_P1013
version: 1
was generated by: iGEM to SBOL conversion
description: gentamycin resistance cassette (aacC1 gene)
creator: Tom Knight
created: 2006-03-16T12:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The aacC1 gene conveys resistance to gentamycin only. It is derived from a Tn21 series transposon (Wohlleben89) found on the R1033 plasmid (Pseudomonas aeruginosa). This part is created by PCR from the pJAT8 plasmid (Keastling lab) which in turn is derived from pJN105 (Newman and Fuqua).
mutable notes: pJAT8 plasmid edited with primers: GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ccttgtcgccttgcgtataa,aacC1-F GTT TCT TCC TGC AGC GGC CGC TAC TAG TA atctcggcttgaacgaattg,aacC1-R
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: aacC1 coding sequence
sequence annotation: RBS
sequence annotation: -35 / -10 promoter sites
sequence: https://synbiohub.org/public/igem/BBa_P1013_sequence/1

aacC1 coding sequence
type: SequenceAnnotation
persistent identity: annotation1826614
display id: annotation1826614
version: 1
top level: gmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826614/range1826614/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826614/range1826614/1
type: Range
persistent identity: range1826614
display id: range1826614
version: 1
direction: direction/forward
top level: gmR
start: 288
end: 821
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1826615
display id: annotation1826615
version: 1
top level: gmR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826615/range1826615/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826615/range1826615/1
type: Range
persistent identity: range1826615
display id: range1826615
version: 1
direction: direction/forward
top level: gmR
start: 276
end: 279
orientation: inline

-35 / -10 promoter sites
type: SequenceAnnotation
persistent identity: annotation1826613
display id: annotation1826613
version: 1
top level: gmR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826613/range1826613/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826613/range1826613/1
type: Range
persistent identity: range1826613
display id: range1826613
version: 1
direction: direction/forward
top level: gmR
start: 71
end: 99
orientation: inline

gmR
type: ComponentDefinition
persistent identity: BBa P1014
display id: BBa_P1014
version: 1
was generated by: iGEM to SBOL conversion
description: gentamicin resistance cassette aph(2)
creator: Tom Knight
created: 2006-06-10T11:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155
mutable notes: XbaI site at 660 removed by mutating c->t which also improves codon usage. gatgatttttacagggatattttagagaacgaaatctac,P1014-XF gtagatttcgttctctaaaatatccctgtaaaaatcatc,P1014-XR
mutable provenance: DQ208936
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: stem_loop
sequence annotation: aph(2)
sequence: https://synbiohub.org/public/igem/BBa_P1014_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2073661
display id: annotation2073661
version: 1
top level: gmR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_P1014/annotation2073661/range2073661/1

https://synbiohub.org/public/igem/BBa_P1014/annotation2073661/range2073661/1
type: Range
persistent identity: range2073661
display id: range2073661
version: 1
direction: direction/forward
top level: gmR
start: 1040
end: 1070
orientation: inline

aph(2)
type: SequenceAnnotation
persistent identity: annotation2073660
display id: annotation2073660
version: 1
top level: gmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1014/annotation2073660/range2073660/1

https://synbiohub.org/public/igem/BBa_P1014/annotation2073660/range2073660/1
type: Range
persistent identity: range2073660
display id: range2073660
version: 1
direction: direction/forward
top level: gmR
start: 120
end: 1025
orientation: inline

pUC19 ori
type: ComponentDefinition
persistent identity: BBa I50020
display id: BBa_I50020
version: 1
was generated by: iGEM to SBOL conversion
description: Minimal pUC19-derived high copy replication origin
created: 2006-01-09T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: RNAII transcript
sequence annotation: G to A, nonfunctional mutation
sequence annotation: origin of replication
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: C to T, nonfunctional mutation
sequence annotation: RNAI transcript promoter -10
sequence annotation: rep (pMB1)
sequence annotation: ORI
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: RNAI transcript
sequence annotation: RNAI transcript promoter -35
sequence: https://synbiohub.org/public/igem/BBa_I50020_sequence/1

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1793681
display id: annotation1793681
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793681/range1793681/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1793681/range1793681/1
type: Range
persistent identity: range1793681
display id: range1793681
version: 1
top level: pUC19 ori
direction: direction/forward
start: 63
end: 615
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910688
display id: annotation1910688
version: 1
top level: pUC19 ori
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910688/range1910688/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1910688/range1910688/1
type: Range
persistent identity: range1910688
display id: range1910688
version: 1
top level: pUC19 ori
direction: direction/forward
start: 265
end: 265
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1793680
display id: annotation1793680
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793680/range1793680/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1793680/range1793680/1
type: Range
persistent identity: range1793680
display id: range1793680
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 615
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1793685
display id: annotation1793685
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793685/range1793685/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1793685/range1793685/1
type: Range
persistent identity: range1793685
display id: range1793685
version: 1
top level: pUC19 ori
direction: direction/forward
start: 48
end: 53
orientation: inline

C to T, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910691
display id: annotation1910691
version: 1
top level: pUC19 ori
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910691/range1910691/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1910691/range1910691/1
type: Range
persistent identity: range1910691
display id: range1910691
version: 1
top level: pUC19 ori
direction: direction/forward
start: 467
end: 467
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910689
display id: annotation1910689
version: 1
top level: pUC19 ori
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910689/range1910689/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1910689/range1910689/1
type: Range
persistent identity: range1910689
display id: range1910689
version: 1
top level: pUC19 ori
direction: direction/forward
start: 274
end: 274
orientation: inline

RNAI transcript promoter -10
type: SequenceAnnotation
persistent identity: annotation1793683
display id: annotation1793683
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793683/range1793683/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1793683/range1793683/1
type: Range
persistent identity: range1793683
display id: range1793683
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 182
end: 187
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910687
display id: annotation1910687
version: 1
top level: pUC19 ori
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910687/range1910687/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1910687/range1910687/1
type: Range
persistent identity: range1910687
display id: range1910687
version: 1
top level: pUC19 ori
direction: direction/forward
start: 261
end: 261
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1782180
display id: annotation1782180
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1782180/range1782180/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1782180/range1782180/1
type: Range
persistent identity: range1782180
display id: range1782180
version: 1
top level: pUC19 ori
direction: direction/forward
start: 16
end: 630
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1782181
display id: annotation1782181
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1782181/range1782181/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1782181/range1782181/1
type: Range
persistent identity: range1782181
display id: range1782181
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 615
end: 615
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1793686
display id: annotation1793686
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793686/range1793686/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1793686/range1793686/1
type: Range
persistent identity: range1793686
display id: range1793686
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 32
orientation: inline

C to T, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910690
display id: annotation1910690
version: 1
top level: pUC19 ori
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910690/range1910690/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1910690/range1910690/1
type: Range
persistent identity: range1910690
display id: range1910690
version: 1
top level: pUC19 ori
direction: direction/forward
start: 303
end: 303
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1793684
display id: annotation1793684
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793684/range1793684/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1793684/range1793684/1
type: Range
persistent identity: range1793684
display id: range1793684
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 66
end: 173
orientation: inline

RNAI transcript promoter -35
type: SequenceAnnotation
persistent identity: annotation1793682
display id: annotation1793682
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793682/range1793682/1

https://synbiohub.org/public/igem/BBa_I50020/annotation1793682/range1793682/1
type: Range
persistent identity: range1793682
display id: range1793682
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 204
end: 209
orientation: inline

cmR
type: ComponentDefinition
persistent identity: BBa P1000
display id: BBa_P1000
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol resistance cassette
creator: Austin Che
created: 2005-12-19T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 22
owning group id: 41
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This provides the entire chloramphenicol resistance operon and will express CmR.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_P1000_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1771290
display id: annotation1771290
version: 1
top level: cmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1000/annotation1771290/range1771290/1

https://synbiohub.org/public/igem/BBa_P1000/annotation1771290/range1771290/1
type: Range
persistent identity: range1771290
display id: range1771290
version: 1
direction: direction/forward
top level: cmR
start: 107
end: 766
orientation: inline

BBa_I52002
type: ComponentDefinition
persistent identity: BBa I52002
display id: BBa_I52002
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB and minimal pUC19 derived high copy origin
created: 2008-01-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: ccdB and pUC19
owned by: james
owned by: myers
mutable description: {| |- |width='35%'| |width='65%'| The CcdB protein, constitutively expressed by P1016, is lethal to most of the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=cell BioBrick cell strains], only DB3.1 is resistant. |} This part is designed to be located in the multiple cloning site of low copy BioBrick vectors. It is composed of two parts BBa_P1016 and BBa_I50022. BBa_P1016 provides selection against non-insert containing clones (see below). BBa_I50022 is based on the high copy pUC19 origin. It enables the base vector to be propagated at high copy for ease of purification. P1016 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die and the surviving colonies are the desired result. I50020 is useful because it is a high copy origin located within the BioBricks cloning site. Any plasmids containing this part will be propagated (and thus purified) at high copy. Once the insert of interest is cloned into the plasmid, the I50022 part is eliminated and the plasmid returns to the copy number of the primary origin in the plasmid (usually low copy). A new set of low copy BioBrick plasmids are available with the I52001 insert. See [http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=I52001 I52001 Physical DNA] for the current list of plasmids that are available in this form.
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: This part is a composite part of BBa_P1016 and BBa_I50022. It is designed to be placed in the multiple cloning site of BioBricks vectors. Vectors with this part in the multiple cloning site will be selected against during BioBricks assembly because BBa_P1016 is lethal to most cell strains. Also, BBa_I50022 enables the vector to be propagated at high copy regardless of the other origins on the vector.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_I50022
component: BBa_P1016
sequence annotation: BBa_P1016
sequence annotation: BBa_I50022
sequence: https://synbiohub.org/public/igem/BBa_I52002_sequence/1

BBa_I50022
type: Component
persistent identity: component1959139
display id: component1959139
version: 1
top level: BBa_I52002
access: public
definition: pUC19 ori

BBa_P1016
type: Component
persistent identity: component1959129
display id: component1959129
version: 1
top level: BBa_I52002
access: public
definition: ccdB

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1959129
display id: annotation1959129
version: 1
top level: BBa_I52002
location: https://synbiohub.org/public/igem/BBa_I52002/annotation1959129/range1959129/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I52002/annotation1959129/range1959129/1
type: Range
persistent identity: range1959129
display id: range1959129
version: 1
top level: BBa_I52002
direction: direction/reverse
start: 1
end: 416
orientation: inline

BBa_I50022
type: SequenceAnnotation
persistent identity: annotation1959139
display id: annotation1959139
version: 1
top level: BBa_I52002
location: https://synbiohub.org/public/igem/BBa_I52002/annotation1959139/range1959139/1
component: BBa_I50022

https://synbiohub.org/public/igem/BBa_I52002/annotation1959139/range1959139/1
type: Range
persistent identity: range1959139
display id: range1959139
version: 1
top level: BBa_I52002
direction: direction/reverse
start: 417
end: 1090
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: polya
sequence annotation: stop
sequence annotation: T7 TE
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa P1010
display id: BBa_P1010
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB cell death gene
created: 2004-07-27T11:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
part status: Deleted
mutable description: The ccd operon having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. This part is used to aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up. P1010 is only provided as plasmid or in DB3.1 which is ccdB resistant.
discontinued: true
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Leon Chan
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T to C mutation
sequence annotation: -10 (rev)
sequence annotation: -35 (rev)
sequence annotation: ccdA- (rev)
sequence annotation: ccdB (rev)
sequence: https://synbiohub.org/public/igem/BBa_P1010_sequence/1

T to C mutation
type: SequenceAnnotation
persistent identity: annotation1747334
display id: annotation1747334
version: 1
top level: ccdB
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747334/range1747334/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747334/range1747334/1
type: Range
persistent identity: range1747334
display id: range1747334
version: 1
top level: ccdB
direction: direction/forward
start: 467
end: 467
orientation: inline

-10 (rev)
type: SequenceAnnotation
persistent identity: annotation1747335
display id: annotation1747335
version: 1
top level: ccdB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747335/range1747335/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747335/range1747335/1
type: Range
persistent identity: range1747335
display id: range1747335
version: 1
top level: ccdB
direction: direction/reverse
start: 595
end: 600
orientation: inline

-35 (rev)
type: SequenceAnnotation
persistent identity: annotation1747336
display id: annotation1747336
version: 1
top level: ccdB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747336/range1747336/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747336/range1747336/1
type: Range
persistent identity: range1747336
display id: range1747336
version: 1
top level: ccdB
direction: direction/reverse
start: 618
end: 623
orientation: inline

ccdA- (rev)
type: SequenceAnnotation
persistent identity: annotation1747333
display id: annotation1747333
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747333/range1747333/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747333/range1747333/1
type: Range
persistent identity: range1747333
display id: range1747333
version: 1
top level: ccdB
direction: direction/reverse
start: 336
end: 556
orientation: inline

ccdB (rev)
type: SequenceAnnotation
persistent identity: annotation1747332
display id: annotation1747332
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1010/annotation1747332/range1747332/1

https://synbiohub.org/public/igem/BBa_P1010/annotation1747332/range1747332/1
type: Range
persistent identity: range1747332
display id: range1747332
version: 1
top level: ccdB
direction: direction/reverse
start: 29
end: 334
orientation: inline

ble
type: ComponentDefinition
persistent identity: BBa K1033990
display id: BBa_K1033990
version: 1
was generated by: iGEM to SBOL conversion
description: Zeocin resistance cassette
creator: Erik Gullberg
created: 2013-09-02T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Zeocoin and bleomycin resistance cassette including promoter and coding sequence. It lacks a terminator.
mutable notes: Codon optimized for E coli by GenScript.
mutable provenance: Streptoalloteichus hindustanus, PDB: 1BYL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ble
sequence annotation: Promoter
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1033990_sequence/1

ble
type: SequenceAnnotation
persistent identity: annotation2334639
display id: annotation2334639
version: 1
top level: ble
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334639/range2334639/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334639/range2334639/1
type: Range
persistent identity: range2334639
display id: range2334639
version: 1
direction: direction/forward
top level: ble
start: 72
end: 446
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2334637
display id: annotation2334637
version: 1
top level: ble
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334637/range2334637/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334637/range2334637/1
type: Range
persistent identity: range2334637
display id: range2334637
version: 1
direction: direction/forward
top level: ble
start: 2
end: 45
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2334638
display id: annotation2334638
version: 1
top level: ble
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334638/range2334638/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334638/range2334638/1
type: Range
persistent identity: range2334638
display id: range2334638
version: 1
direction: direction/forward
top level: ble
start: 58
end: 64
orientation: inline

BBa_I52001
type: ComponentDefinition
persistent identity: BBa I52001
display id: BBa_I52001
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB and nonfunctional pUC19 derived high copy origin
created: 2007-01-29T12:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: ccdB and pUC19
owned by: james
owned by: myers
mutable description: This part is designed to be located in the multiple cloning site of low copy BioBrick vectors. BBa_P1016 provides selection against non-insert containing clones and BBa_I50020 enables the base vector to be propagated at high copy for ease of purification
discontinued: true
dominant: false
experience: experience/Issues
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: This part is a composite part of BBa_P1016 and BBa_I50020. It is designed to be placed in the multiple cloning site of BioBricks vectors. Vectors with this part in the multiple cloning site will be selected against during BioBricks assembly because BBa_P1016 is lethal to most cell strains. Also, BBa_I50020 enables the vector to be propagated at high copy regardless of the other origins on the vector.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_P1016
component: BBa_I50020
sequence annotation: BBa_P1016
sequence annotation: BBa_I50020
sequence: https://synbiohub.org/public/igem/BBa_I52001_sequence/1

BBa_P1016
type: Component
persistent identity: component1910763
display id: component1910763
version: 1
top level: BBa_I52001
access: public
definition: ccdB

BBa_I50020
type: Component
persistent identity: component1910778
display id: component1910778
version: 1
top level: BBa_I52001
access: public
definition: pUC19 ori

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1910763
display id: annotation1910763
version: 1
top level: BBa_I52001
location: https://synbiohub.org/public/igem/BBa_I52001/annotation1910763/range1910763/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I52001/annotation1910763/range1910763/1
type: Range
persistent identity: range1910763
display id: range1910763
version: 1
top level: BBa_I52001
direction: direction/reverse
start: 1
end: 416
orientation: inline

BBa_I50020
type: SequenceAnnotation
persistent identity: annotation1910778
display id: annotation1910778
version: 1
top level: BBa_I52001
location: https://synbiohub.org/public/igem/BBa_I52001/annotation1910778/range1910778/1
component: BBa_I50020

https://synbiohub.org/public/igem/BBa_I52001/annotation1910778/range1910778/1
type: Range
persistent identity: range1910778
display id: range1910778
version: 1
top level: BBa_I52001
direction: direction/reverse
start: 417
end: 1090
orientation: inline

ampR
type: ComponentDefinition
persistent identity: BBa P1002
display id: BBa_P1002
version: 1
was generated by: iGEM to SBOL conversion
description: ampicillin resistance cassette
creator: Reshma Shetty
created: 2006-01-23T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ampicillin resistance cassette for modular BioBricks vector construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ampicillin resistance
sequence annotation: ampR -10
sequence annotation: ampR -35
sequence annotation: ampR RBS
sequence: https://synbiohub.org/public/igem/BBa_P1002_sequence/1

ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation1790786
display id: annotation1790786
version: 1
top level: ampR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1790786/range1790786/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1790786/range1790786/1
type: Range
persistent identity: range1790786
display id: range1790786
version: 1
direction: direction/forward
top level: ampR
start: 80
end: 943
orientation: inline

ampR -10
type: SequenceAnnotation
persistent identity: annotation1897110
display id: annotation1897110
version: 1
top level: ampR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897110/range1897110/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1897110/range1897110/1
type: Range
persistent identity: range1897110
display id: range1897110
version: 1
direction: direction/forward
top level: ampR
start: 57
end: 62
orientation: inline

ampR -35
type: SequenceAnnotation
persistent identity: annotation1897109
display id: annotation1897109
version: 1
top level: ampR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897109/range1897109/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1897109/range1897109/1
type: Range
persistent identity: range1897109
display id: range1897109
version: 1
direction: direction/forward
top level: ampR
start: 31
end: 36
orientation: inline

ampR RBS
type: SequenceAnnotation
persistent identity: annotation1897111
display id: annotation1897111
version: 1
top level: ampR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897111/range1897111/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1897111/range1897111/1
type: Range
persistent identity: range1897111
display id: range1897111
version: 1
direction: direction/forward
top level: ampR
start: 71
end: 75
orientation: inline

GmR
type: ComponentDefinition
persistent identity: BBa P1012
display id: BBa_P1012
version: 1
was generated by: iGEM to SBOL conversion
description: gentamycin/kanamycin resistance cassette
creator: Tom Knight
created: 2006-03-15T12:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The aacA-aphD gene from the transposon Tn4001 conveys resistance to both Gentamycin and Kanamycin with a single two-domain resistance protein.
mutable notes: ;primers to make Biobrick version of GmR gene cassette (P1012) gtttcttcgaattcgcggccgcttctagagTGGACTTGACTGAGTTTATGGAA, P3aph-F gtttcttcctgcagcggccgctactagtaTCTGGACTTGACTCACTTCCTTT, P3aph-R ;P3aph-R (3' - 5') ;aaaggaagtgagtcaagtccagatactagtagcggccgctgcaggaagaaac
mutable provenance: Originally from Tn4001 transposon; sequence M18086. Created from plasmid pISM2062 from Chris Minion, Iowa State University.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: acetyl-transferase
sequence annotation: aacA-aphD (GmR, KanR)
sequence: https://synbiohub.org/public/igem/BBa_P1012_sequence/1

acetyl-transferase
type: SequenceAnnotation
persistent identity: annotation1825004
display id: annotation1825004
version: 1
top level: GmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1012/annotation1825004/range1825004/1

https://synbiohub.org/public/igem/BBa_P1012/annotation1825004/range1825004/1
type: Range
persistent identity: range1825004
display id: range1825004
version: 1
direction: direction/forward
top level: GmR
start: 81
end: 411
orientation: inline

aacA-aphD (GmR, KanR)
type: SequenceAnnotation
persistent identity: annotation1825005
display id: annotation1825005
version: 1
top level: GmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1012/annotation1825005/range1825005/1

https://synbiohub.org/public/igem/BBa_P1012/annotation1825005/range1825005/1
type: Range
persistent identity: range1825005
display id: range1825005
version: 1
direction: direction/forward
top level: GmR
start: 412
end: 1851
orientation: inline

AD default
type: ComponentDefinition
persistent identity: BBa K106400
display id: BBa_K106400
version: 1
was generated by: iGEM to SBOL conversion
description: Default insert for AarI AD acceptor vectors
created: 2008-11-24T12:00:00Z
modified: 2015-05-08T01:08:58Z
mutable provenance: AarI AD acceptor vectors
owned by: james
owned by: myers
mutable description: Default insert for AarI AD acceptor vectors. It includes the AarI restriction sites but not the 4 bp D or A overhangs.
discontinued: false
dominant: false
experience: experience/None
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: It includes the AarI restriction sites but not the 4 bp D or A overhangs.
star: false
creator: Sergio Peisajovich
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Aar I recognition site
sequence annotation: AarI recognition site
sequence: https://synbiohub.org/public/igem/BBa_K106400_sequence/1

Aar I recognition site
type: SequenceAnnotation
persistent identity: annotation1999684
display id: annotation1999684
version: 1
top level: AD default
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106400/annotation1999684/range1999684/1

https://synbiohub.org/public/igem/BBa_K106400/annotation1999684/range1999684/1
type: Range
persistent identity: range1999684
display id: range1999684
version: 1
top level: AD default
direction: direction/forward
start: 42
end: 48
orientation: inline

AarI recognition site
type: SequenceAnnotation
persistent identity: annotation1999683
display id: annotation1999683
version: 1
top level: AD default
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106400/annotation1999683/range1999683/1

https://synbiohub.org/public/igem/BBa_K106400/annotation1999683/range1999683/1
type: Range
persistent identity: range1999683
display id: range1999683
version: 1
top level: AD default
direction: direction/reverse
start: 5
end: 11
orientation: inline

TetR
type: ComponentDefinition
persistent identity: BBa P1001
display id: BBa_P1001
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance cassette
creator: Austin Che
created: 2005-12-19T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 22
owning group id: 41
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This cassette provides the entire tetracycline resistance operon and will express TetR.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: tetA
sequence annotation: tetA promoter
sequence: https://synbiohub.org/public/igem/BBa_P1001_sequence/1

tetA
type: SequenceAnnotation
persistent identity: annotation1771321
display id: annotation1771321
version: 1
top level: TetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1001/annotation1771321/range1771321/1

https://synbiohub.org/public/igem/BBa_P1001/annotation1771321/range1771321/1
type: Range
persistent identity: range1771321
display id: range1771321
version: 1
direction: direction/forward
top level: TetR
start: 89
end: 1279
orientation: inline

tetA promoter
type: SequenceAnnotation
persistent identity: annotation1771322
display id: annotation1771322
version: 1
top level: TetR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1001/annotation1771322/range1771322/1

https://synbiohub.org/public/igem/BBa_P1001/annotation1771322/range1771322/1
type: Range
persistent identity: range1771322
display id: range1771322
version: 1
direction: direction/forward
top level: TetR
start: 1
end: 88
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J61000
display id: BBa_J61000
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol resistance cassette
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chloramphenicol resistance gene including its native promoter and ribosome binding site. Confers resistance to 25 ug/mL chloramphenicol. {pSB1A2-Bca1016}
mutable notes: N/A
mutable provenance: PCR ca1016F/R on pKD3 (934 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Products are Bca1015 and Bca1016 ---- ca1016F Forward EcoRI to biobrick CAT of pKD3 gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg ca1016R Reverse SpeI to biobrick CAT of pKD3 ccgctactagtggcgcgcctacctgtgacgg
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_J61000_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1892270
display id: annotation1892270
version: 1
top level: CmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1

https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1
type: Range
persistent identity: range1892270
display id: range1892270
version: 1
direction: direction/reverse
top level: CmR
start: 30
end: 687
orientation: inline

PcpcB-Kana
type: ComponentDefinition
persistent identity: BBa K1642003
display id: BBa_K1642003
version: 1
was generated by: iGEM to SBOL conversion
description: Kanamycin Resistance Cassette in Cyanobacteria
creator: Can Yin
created: 2015-08-14T11:00:00Z
modified: 2015-09-18T04:39:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2059_
m user id: 24423
owner id: 24405
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Constitutively
mutable notes: Kana
mutable provenance: 6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: KanR
sequence annotation: PcpcB
sequence: https://synbiohub.org/public/igem/BBa_K1642003_sequence/1

KanR
type: SequenceAnnotation
persistent identity: annotation2435670
display id: annotation2435670
version: 1
top level: PcpcB-Kana
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1642003/annotation2435670/range2435670/1

https://synbiohub.org/public/igem/BBa_K1642003/annotation2435670/range2435670/1
type: Range
persistent identity: range2435670
display id: range2435670
version: 1
direction: direction/forward
top level: PcpcB-Kana
start: 590
end: 1402
orientation: inline

PcpcB
type: SequenceAnnotation
persistent identity: annotation2435669
display id: annotation2435669
version: 1
top level: PcpcB-Kana
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1642003/annotation2435669/range2435669/1

https://synbiohub.org/public/igem/BBa_K1642003/annotation2435669/range2435669/1
type: Range
persistent identity: range2435669
display id: range2435669
version: 1
direction: direction/forward
top level: PcpcB-Kana
start: 1
end: 589
orientation: inline

OnRFP
type: ComponentDefinition
persistent identity: BBa J23025
display id: BBa_J23025
version: 1
was generated by: iGEM to SBOL conversion
description: OnRFP
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: Dropped
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Dropped
discontinued: false
dominant: false
experience: experience/None
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Dropped
star: false
creator: John Anderson
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rrnB T1 terminator
sequence annotation: OnRFP
sequence annotation: Coding sequence
sequence: https://synbiohub.org/public/igem/BBa_J23025_sequence/1

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975733
display id: annotation1975733
version: 1
top level: OnRFP
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J23025/annotation1975733/range1975733/1

https://synbiohub.org/public/igem/BBa_J23025/annotation1975733/range1975733/1
type: Range
persistent identity: range1975733
display id: range1975733
version: 1
top level: OnRFP
direction: direction/forward
start: 806
end: 849
orientation: inline

OnRFP
type: SequenceAnnotation
persistent identity: annotation1975731
display id: annotation1975731
version: 1
top level: OnRFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J23025/annotation1975731/range1975731/1

https://synbiohub.org/public/igem/BBa_J23025/annotation1975731/range1975731/1
type: Range
persistent identity: range1975731
display id: range1975731
version: 1
top level: OnRFP
direction: direction/forward
start: 1
end: 923
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation1975732
display id: annotation1975732
version: 1
top level: OnRFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23025/annotation1975732/range1975732/1

https://synbiohub.org/public/igem/BBa_J23025/annotation1975732/range1975732/1
type: Range
persistent identity: range1975732
display id: range1975732
version: 1
top level: OnRFP
direction: direction/forward
start: 81
end: 761
orientation: inline

tetA
type: ComponentDefinition
persistent identity: BBa P1005
display id: BBa_P1005
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance cassette
creator: Reshma Shetty
created: 2006-02-03T12:00:00Z
modified: 2015-08-21T03:01:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 8248
owner id: 126
owning group id: 45
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This contains CmR, a terminator and then AmpR and a terminator.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: tetR promoter
sequence annotation: tetA
sequence: https://synbiohub.org/public/igem/BBa_P1005_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1897140
display id: annotation1897140
version: 1
top level: tetA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1005/annotation1897140/range1897140/1

https://synbiohub.org/public/igem/BBa_P1005/annotation1897140/range1897140/1
type: Range
persistent identity: range1897140
display id: range1897140
version: 1
direction: direction/forward
top level: tetA
start: 1
end: 89
orientation: inline

tetA
type: SequenceAnnotation
persistent identity: annotation1897141
display id: annotation1897141
version: 1
top level: tetA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1005/annotation1897141/range1897141/1

https://synbiohub.org/public/igem/BBa_P1005/annotation1897141/range1897141/1
type: Range
persistent identity: range1897141
display id: range1897141
version: 1
direction: direction/forward
top level: tetA
start: 90
end: 1283
orientation: inline

kanR
type: ComponentDefinition
persistent identity: BBa P1003
display id: BBa_P1003
version: 1
was generated by: iGEM to SBOL conversion
description: kanamycin resistance cassette
creator: Reshma Shetty
created: 2006-01-23T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: In stock
status: status/Available
bookmark: false
mutable description: Kanamycin resistance cassette for modular BioBricks vector construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: kanamycin resistance
sequence: https://synbiohub.org/public/igem/BBa_P1003_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation1897112
display id: annotation1897112
version: 1
top level: kanR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1

https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1
type: Range
persistent identity: range1897112
display id: range1897112
version: 1
direction: direction/forward
top level: kanR
start: 149
end: 967
orientation: inline

BBa_K1937002
type: ComponentDefinition
persistent identity: BBa K1937002
display id: BBa_K1937002
version: 1
was generated by: iGEM to SBOL conversion
description: OriKan: a composite part to turn any pSB1C3 into a Bacillus plasmid
creator: Brice Enjalbert
created: 2016-10-12T11:00:00Z
modified: 2016-10-15T03:11:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2404_
m user id: 21497
owner id: 21497
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Part: BBa_K1937002 (pSB1C3-OriKan) (Chassis E. coli/B. subtilis, carrier plasmid pSB1C3) Length: 2510 bp While Bacillus subtilis is of huge interest for a growing number of iGEM projects, it is not easy to develop new parts as it is required that they registered after sub-cloning in the E. coli plasmid pSB1C3. During the iGEM Toulouse 2016 project, we had the idea to create a part that could turn any pSB1C3-based plasmid in a shuttle vector (E. coli/B. subtilis). This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
mutable notes: We successfully transformed E. coli and selected clones on chloramphenicol. Likewise, we successfully transformed B. subtilis and selected clones on kanamycine. Presence of the pSB1C3-OriKan plasmid was demonstrated in B. subtilis by PCR checking. The part sequence has been verified by sequencing.
mutable provenance: The BBa_K1937002 part contains the repU origin of replication of B. subtilis and the kanamycin resistance gene for B. subtilis. It was obtained by amplifying the repU-Kan region of pSBBsOK_P (BBa_K1351040) using primers OriKan forward (5??? cacagaatcaggggataacgcaggaaagaaACATGTAGTTATAAGTGACTAAACAAATAACTAAATAGATGGG) and OriKan reverse (5??? gttcctggccttttgctggccttttgctcaACATGTCGCAAAATGGCCCGATTTAAG). The resulting fragment was sub-cloned in the pSB1C3 between the EcoRI and PstI restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RepU Bacillus origin
sequence annotation: Kananmycin resistance (Bacillus)
sequence: https://synbiohub.org/public/igem/BBa_K1937002_sequence/1

RepU Bacillus origin
type: SequenceAnnotation
persistent identity: annotation2497331
display id: annotation2497331
version: 1
top level: BBa_K1937002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1937002/annotation2497331/range2497331/1

https://synbiohub.org/public/igem/BBa_K1937002/annotation2497331/range2497331/1
type: Range
persistent identity: range2497331
display id: range2497331
version: 1
direction: direction/forward
top level: BBa_K1937002
start: 431
end: 1435
orientation: inline

Kananmycin resistance (Bacillus)
type: SequenceAnnotation
persistent identity: annotation2497330
display id: annotation2497330
version: 1
top level: BBa_K1937002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1937002/annotation2497330/range2497330/1

https://synbiohub.org/public/igem/BBa_K1937002/annotation2497330/range2497330/1
type: Range
persistent identity: range2497330
display id: range2497330
version: 1
direction: direction/forward
top level: BBa_K1937002
start: 1595
end: 2365
orientation: inline

BBa_K292003
type: ComponentDefinition
persistent identity: BBa K292003
display id: BBa_K292003
version: 1
was generated by: iGEM to SBOL conversion
description: Kanamycin resistance cassette and a double terminator
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a kanamycin resistance cassette and a double terminator (BBa_P1003 + BBa_B0014). This allows the kanamycin cassette to be functional. The cassette can be used to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage. In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin. This part is completed by B0014 + P1003 + B0014 to finish the transcription before the resistance cassette.
mutable notes: When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.
mutable provenance: Part:BBa_P1003, Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-23) Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_P1003
component: BBa_B0012
component: BBa_B0011
sequence annotation: BBa_P1003
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_K292003_sequence/1

BBa_P1003
type: Component
persistent identity: component2054557
display id: component2054557
version: 1
top level: BBa_K292003
access: public
definition: kanR

BBa_B0012
type: Component
persistent identity: component2054558
display id: component2054558
version: 1
top level: BBa_K292003
access: public
definition: BBa_B0012

BBa_B0011
type: Component
persistent identity: component2054562
display id: component2054562
version: 1
top level: BBa_K292003
access: public
definition: BBa_B0011

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2054557
display id: annotation2054557
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054557/range2054557/1
component: BBa_P1003

https://synbiohub.org/public/igem/BBa_K292003/annotation2054557/range2054557/1
type: Range
persistent identity: range2054557
display id: range2054557
version: 1
direction: direction/reverse
top level: BBa_K292003
start: 1
end: 967
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054558
display id: annotation2054558
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054558/range2054558/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292003/annotation2054558/range2054558/1
type: Range
persistent identity: range2054558
display id: range2054558
version: 1
direction: direction/forward
top level: BBa_K292003
start: 976
end: 1016
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054562
display id: annotation2054562
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054562/range2054562/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292003/annotation2054562/range2054562/1
type: Range
persistent identity: range2054562
display id: range2054562
version: 1
direction: direction/forward
top level: BBa_K292003
start: 1025
end: 1070
orientation: inline

cmR
type: ComponentDefinition
persistent identity: BBa P1004
display id: BBa_P1004
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol resistance cassette
creator: Reshma Shetty
created: 2006-02-03T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same part as BBa_P1002 except in reverse orientation.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: cmR
sequence: https://synbiohub.org/public/igem/BBa_P1004_sequence/1

cmR
type: SequenceAnnotation
persistent identity: annotation1897114
display id: annotation1897114
version: 1
top level: cmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1004/annotation1897114/range1897114/1

https://synbiohub.org/public/igem/BBa_P1004/annotation1897114/range1897114/1
type: Range
persistent identity: range1897114
display id: range1897114
version: 1
direction: direction/forward
top level: cmR
start: 107
end: 769
orientation: inline

BBa_K292004
type: ComponentDefinition
persistent identity: BBa K292004
display id: BBa_K292004
version: 1
was generated by: iGEM to SBOL conversion
description: Double terminator + Kanamycin resistance cassette + Double ter
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a double terminator and a functional kanamycin resistance cassette (promoter, resistance against kanamycin and a double terminator, BBa_B0014 + BBa_P1003 + BBa_B0014). It is reliable to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage. In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin. We can use this part as a reporter, or as a simple resistance cassette. The double terminator allows the RNA translation arrest before the resistance cassette, so this is already created to insert a coding gene with its RBS before the resistance cassette.
mutable notes: When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.
mutable provenance: Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16) Part:BBa_P1003, Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-23)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_P1003
component: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_P1003
sequence: https://synbiohub.org/public/igem/BBa_K292004_sequence/1

BBa_B0012
type: Component
persistent identity: component2054576
display id: component2054576
version: 1
top level: BBa_K292004
access: public
definition: BBa_B0012

BBa_B0012
type: Component
persistent identity: component2054568
display id: component2054568
version: 1
top level: BBa_K292004
access: public
definition: BBa_B0012

BBa_P1003
type: Component
persistent identity: component2054575
display id: component2054575
version: 1
top level: BBa_K292004
access: public
definition: kanR

BBa_B0011
type: Component
persistent identity: component2054580
display id: component2054580
version: 1
top level: BBa_K292004
access: public
definition: BBa_B0011

BBa_B0011
type: Component
persistent identity: component2054572
display id: component2054572
version: 1
top level: BBa_K292004
access: public
definition: BBa_B0011

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054568
display id: annotation2054568
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054568/range2054568/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292004/annotation2054568/range2054568/1
type: Range
persistent identity: range2054568
display id: range2054568
version: 1
direction: direction/forward
top level: BBa_K292004
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054572
display id: annotation2054572
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054572/range2054572/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292004/annotation2054572/range2054572/1
type: Range
persistent identity: range2054572
display id: range2054572
version: 1
direction: direction/forward
top level: BBa_K292004
start: 50
end: 95
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054580
display id: annotation2054580
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054580/range2054580/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292004/annotation2054580/range2054580/1
type: Range
persistent identity: range2054580
display id: range2054580
version: 1
direction: direction/forward
top level: BBa_K292004
start: 1128
end: 1173
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2054575
display id: annotation2054575
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054575/range2054575/1
component: BBa_P1003

https://synbiohub.org/public/igem/BBa_K292004/annotation2054575/range2054575/1
type: Range
persistent identity: range2054575
display id: range2054575
version: 1
direction: direction/reverse
top level: BBa_K292004
start: 104
end: 1070
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054576
display id: annotation2054576
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054576/range2054576/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292004/annotation2054576/range2054576/1
type: Range
persistent identity: range2054576
display id: range2054576
version: 1
direction: direction/forward
top level: BBa_K292004
start: 1079
end: 1119
orientation: inline

Default in
type: ComponentDefinition
persistent identity: BBa I739400
display id: BBa_I739400
version: 1
was generated by: iGEM to SBOL conversion
description: Default insert of plasmid BBa_I739201, BBa_I739202, BBa_I739204
created: 2008-09-02T11:00:00Z
modified: 2015-08-31T04:08:01Z
mutable provenance: BBa_I739201
owned by: james
owned by: myers
mutable description: This is simply a default vector insert.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I739400_sequence/1

pUC19 ori
type: ComponentDefinition
persistent identity: BBa I50022
display id: BBa_I50022
version: 1
was generated by: iGEM to SBOL conversion
description: Minimal pUC19-derived high copy replication origin
created: 2008-01-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: pSB1A3
owned by: james
owned by: myers
mutable description: High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites. Lacks mutations that render BBa_I50020 nonfunctional as a replication origin.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We had to leave several restriction sites in because point mutations that eliminated these sites were found to render the origin nonfunctional.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RNAI transcript promoter -10
sequence annotation: rep (pMB1)
sequence annotation: RNAII transcript
sequence annotation: RNAI transcript promoter -35
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: RNAI transcript
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: origin of replication
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_I50022_sequence/1

RNAI transcript promoter -10
type: SequenceAnnotation
persistent identity: annotation1959123
display id: annotation1959123
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959123/range1959123/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959123/range1959123/1
type: Range
persistent identity: range1959123
display id: range1959123
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 182
end: 187
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1959117
display id: annotation1959117
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959117/range1959117/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959117/range1959117/1
type: Range
persistent identity: range1959117
display id: range1959117
version: 1
top level: pUC19 ori
direction: direction/forward
start: 16
end: 630
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1959121
display id: annotation1959121
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959121/range1959121/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959121/range1959121/1
type: Range
persistent identity: range1959121
display id: range1959121
version: 1
top level: pUC19 ori
direction: direction/forward
start: 63
end: 615
orientation: inline

RNAI transcript promoter -35
type: SequenceAnnotation
persistent identity: annotation1959124
display id: annotation1959124
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959124/range1959124/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959124/range1959124/1
type: Range
persistent identity: range1959124
display id: range1959124
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 204
end: 209
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1959120
display id: annotation1959120
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959120/range1959120/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959120/range1959120/1
type: Range
persistent identity: range1959120
display id: range1959120
version: 1
top level: pUC19 ori
direction: direction/forward
start: 48
end: 53
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1959122
display id: annotation1959122
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959122/range1959122/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959122/range1959122/1
type: Range
persistent identity: range1959122
display id: range1959122
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 66
end: 173
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1959118
display id: annotation1959118
version: 1
top level: pUC19 ori
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959118/range1959118/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959118/range1959118/1
type: Range
persistent identity: range1959118
display id: range1959118
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 32
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1959119
display id: annotation1959119
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959119/range1959119/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959119/range1959119/1
type: Range
persistent identity: range1959119
display id: range1959119
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 615
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1959125
display id: annotation1959125
version: 1
top level: pUC19 ori
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959125/range1959125/1

https://synbiohub.org/public/igem/BBa_I50022/annotation1959125/range1959125/1
type: Range
persistent identity: range1959125
display id: range1959125
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 615
end: 615
orientation: inline

https://synbiohub.org/public/igem/BBa_J23025_sequence/1
type: Sequence
persistent identity: BBa J23025 sequence
display id: BBa_J23025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K106400_sequence/1
type: Sequence
persistent identity: BBa K106400 sequence
display id: BBa_K106400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caaggcaggtggacaagaggagtcccgggagctggaactcccacctgcaaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50022_sequence/1
type: Sequence
persistent identity: BBa I50022 sequence
display id: BBa_I50022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1010_sequence/1
type: Sequence
persistent identity: BBa P1010 sequence
display id: BBa_P1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actggctgtgtataagggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccacgcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50020_sequence/1
type: Sequence
persistent identity: BBa I50020 sequence
display id: BBa_I50020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I52002_sequence/1
type: Sequence
persistent identity: BBa I52002 sequence
display id: BBa_I52002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataacccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I52001_sequence/1
type: Sequence
persistent identity: BBa I52001 sequence
display id: BBa_I52001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataacccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1016_sequence/1
type: Sequence
persistent identity: BBa P1016 sequence
display id: BBa_P1016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I739400_sequence/1
type: Sequence
persistent identity: BBa I739400 sequence
display id: BBa_I739400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcctaggcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z