//plasmidbackbone/component/selectionmarker/antibioticresistance
type: Collection
persistent identity: plasmidbackbone component selectionmarker antibioticresistance
display id: plasmidbackbone_component_selectionmarker_antibioticresistance
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: gmR
member: BBa_K292003
member: cmR
member: ampR
member: ble
member: BBa_K1937002
member: BBa_K292004
member: GmR
member: PcpcB-Kana
member: tetA
member: kanR
member: CmR
member: TetR

cmR
type: ComponentDefinition
persistent identity: BBa P1000
display id: BBa_P1000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol resistance cassette
creator: Austin Che
created: 2005-12-19T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 22
owning group id: 41
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This provides the entire chloramphenicol resistance operon and will express CmR.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_P1000_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1771290
display id: annotation1771290
version: 1
top level: cmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1000/annotation1771290/range1771290/1

https://synbiohub.org/public/igem/BBa_P1000/annotation1771290/range1771290/1
type: Range
persistent identity: range1771290
display id: range1771290
version: 1
direction: direction/forward
top level: cmR
start: 107
end: 766
orientation: inline

kanR
type: ComponentDefinition
persistent identity: BBa P1003
display id: BBa_P1003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kanamycin resistance cassette
creator: Reshma Shetty
created: 2006-01-23T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: In stock
status: status/Available
bookmark: false
mutable description: Kanamycin resistance cassette for modular BioBricks vector construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: kanamycin resistance
sequence: https://synbiohub.org/public/igem/BBa_P1003_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation1897112
display id: annotation1897112
version: 1
top level: kanR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1

https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1
type: Range
persistent identity: range1897112
display id: range1897112
version: 1
direction: direction/forward
top level: kanR
start: 149
end: 967
orientation: inline

BBa_K292003
type: ComponentDefinition
persistent identity: BBa K292003
display id: BBa_K292003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kanamycin resistance cassette and a double terminator
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a kanamycin resistance cassette and a double terminator (BBa_P1003 + BBa_B0014). This allows the kanamycin cassette to be functional. The cassette can be used to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage. In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin. This part is completed by B0014 + P1003 + B0014 to finish the transcription before the resistance cassette.
mutable notes: When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.
mutable provenance: Part:BBa_P1003, Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-23) Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_P1003
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_P1003
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K292003_sequence/1

BBa_P1003
type: Component
persistent identity: component2054557
display id: component2054557
version: 1
top level: BBa_K292003
access: public
definition: kanR

BBa_B0011
type: Component
persistent identity: component2054562
display id: component2054562
version: 1
top level: BBa_K292003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2054558
display id: component2054558
version: 1
top level: BBa_K292003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2054557
display id: annotation2054557
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054557/range2054557/1
component: BBa_P1003

https://synbiohub.org/public/igem/BBa_K292003/annotation2054557/range2054557/1
type: Range
persistent identity: range2054557
display id: range2054557
version: 1
direction: direction/reverse
top level: BBa_K292003
start: 1
end: 967
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054562
display id: annotation2054562
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054562/range2054562/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292003/annotation2054562/range2054562/1
type: Range
persistent identity: range2054562
display id: range2054562
version: 1
direction: direction/forward
top level: BBa_K292003
start: 1025
end: 1070
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054558
display id: annotation2054558
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054558/range2054558/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292003/annotation2054558/range2054558/1
type: Range
persistent identity: range2054558
display id: range2054558
version: 1
direction: direction/forward
top level: BBa_K292003
start: 976
end: 1016
orientation: inline

GmR
type: ComponentDefinition
persistent identity: BBa P1012
display id: BBa_P1012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gentamycin/kanamycin resistance cassette
creator: Tom Knight
created: 2006-03-15T12:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The aacA-aphD gene from the transposon Tn4001 conveys resistance to both Gentamycin and Kanamycin with a single two-domain resistance protein.
mutable notes: ;primers to make Biobrick version of GmR gene cassette (P1012) gtttcttcgaattcgcggccgcttctagagTGGACTTGACTGAGTTTATGGAA, P3aph-F gtttcttcctgcagcggccgctactagtaTCTGGACTTGACTCACTTCCTTT, P3aph-R ;P3aph-R (3' - 5') ;aaaggaagtgagtcaagtccagatactagtagcggccgctgcaggaagaaac
mutable provenance: Originally from Tn4001 transposon; sequence M18086. Created from plasmid pISM2062 from Chris Minion, Iowa State University.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: acetyl-transferase
sequence annotation: aacA-aphD (GmR, KanR)
sequence: https://synbiohub.org/public/igem/BBa_P1012_sequence/1

acetyl-transferase
type: SequenceAnnotation
persistent identity: annotation1825004
display id: annotation1825004
version: 1
top level: GmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1012/annotation1825004/range1825004/1

https://synbiohub.org/public/igem/BBa_P1012/annotation1825004/range1825004/1
type: Range
persistent identity: range1825004
display id: range1825004
version: 1
direction: direction/forward
top level: GmR
start: 81
end: 411
orientation: inline

aacA-aphD (GmR, KanR)
type: SequenceAnnotation
persistent identity: annotation1825005
display id: annotation1825005
version: 1
top level: GmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1012/annotation1825005/range1825005/1

https://synbiohub.org/public/igem/BBa_P1012/annotation1825005/range1825005/1
type: Range
persistent identity: range1825005
display id: range1825005
version: 1
direction: direction/forward
top level: GmR
start: 412
end: 1851
orientation: inline

tetA
type: ComponentDefinition
persistent identity: BBa P1005
display id: BBa_P1005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline resistance cassette
creator: Reshma Shetty
created: 2006-02-03T12:00:00Z
modified: 2015-08-21T03:01:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 8248
owner id: 126
owning group id: 45
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This contains CmR, a terminator and then AmpR and a terminator.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: tetA
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_P1005_sequence/1

tetA
type: SequenceAnnotation
persistent identity: annotation1897141
display id: annotation1897141
version: 1
top level: tetA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1005/annotation1897141/range1897141/1

https://synbiohub.org/public/igem/BBa_P1005/annotation1897141/range1897141/1
type: Range
persistent identity: range1897141
display id: range1897141
version: 1
direction: direction/forward
top level: tetA
start: 90
end: 1283
orientation: inline

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1897140
display id: annotation1897140
version: 1
top level: tetA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1005/annotation1897140/range1897140/1

https://synbiohub.org/public/igem/BBa_P1005/annotation1897140/range1897140/1
type: Range
persistent identity: range1897140
display id: range1897140
version: 1
direction: direction/forward
top level: tetA
start: 1
end: 89
orientation: inline

ble
type: ComponentDefinition
persistent identity: BBa K1033990
display id: BBa_K1033990
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zeocin resistance cassette
creator: Erik Gullberg
created: 2013-09-02T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Zeocoin and bleomycin resistance cassette including promoter and coding sequence. It lacks a terminator.
mutable notes: Codon optimized for E coli by GenScript.
mutable provenance: Streptoalloteichus hindustanus, PDB: 1BYL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ble
sequence annotation: Promoter
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1033990_sequence/1

ble
type: SequenceAnnotation
persistent identity: annotation2334639
display id: annotation2334639
version: 1
top level: ble
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334639/range2334639/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334639/range2334639/1
type: Range
persistent identity: range2334639
display id: range2334639
version: 1
direction: direction/forward
top level: ble
start: 72
end: 446
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2334637
display id: annotation2334637
version: 1
top level: ble
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334637/range2334637/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334637/range2334637/1
type: Range
persistent identity: range2334637
display id: range2334637
version: 1
direction: direction/forward
top level: ble
start: 2
end: 45
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2334638
display id: annotation2334638
version: 1
top level: ble
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334638/range2334638/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334638/range2334638/1
type: Range
persistent identity: range2334638
display id: range2334638
version: 1
direction: direction/forward
top level: ble
start: 58
end: 64
orientation: inline

BBa_K292004
type: ComponentDefinition
persistent identity: BBa K292004
display id: BBa_K292004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double terminator + Kanamycin resistance cassette + Double ter
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a double terminator and a functional kanamycin resistance cassette (promoter, resistance against kanamycin and a double terminator, BBa_B0014 + BBa_P1003 + BBa_B0014). It is reliable to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage. In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin. We can use this part as a reporter, or as a simple resistance cassette. The double terminator allows the RNA translation arrest before the resistance cassette, so this is already created to insert a coding gene with its RBS before the resistance cassette.
mutable notes: When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.
mutable provenance: Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16) Part:BBa_P1003, Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-23)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0011
component: BBa_B0012
component: BBa_P1003
sequence annotation: BBa_B0011
sequence annotation: BBa_P1003
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K292004_sequence/1

BBa_B0011
type: Component
persistent identity: component2054580
display id: component2054580
version: 1
top level: BBa_K292004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2054576
display id: component2054576
version: 1
top level: BBa_K292004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_P1003
type: Component
persistent identity: component2054575
display id: component2054575
version: 1
top level: BBa_K292004
access: public
definition: kanR

BBa_B0012
type: Component
persistent identity: component2054568
display id: component2054568
version: 1
top level: BBa_K292004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component2054572
display id: component2054572
version: 1
top level: BBa_K292004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054572
display id: annotation2054572
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054572/range2054572/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292004/annotation2054572/range2054572/1
type: Range
persistent identity: range2054572
display id: range2054572
version: 1
direction: direction/forward
top level: BBa_K292004
start: 50
end: 95
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2054575
display id: annotation2054575
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054575/range2054575/1
component: BBa_P1003

https://synbiohub.org/public/igem/BBa_K292004/annotation2054575/range2054575/1
type: Range
persistent identity: range2054575
display id: range2054575
version: 1
direction: direction/reverse
top level: BBa_K292004
start: 104
end: 1070
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054576
display id: annotation2054576
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054576/range2054576/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292004/annotation2054576/range2054576/1
type: Range
persistent identity: range2054576
display id: range2054576
version: 1
direction: direction/forward
top level: BBa_K292004
start: 1079
end: 1119
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054580
display id: annotation2054580
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054580/range2054580/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292004/annotation2054580/range2054580/1
type: Range
persistent identity: range2054580
display id: range2054580
version: 1
direction: direction/forward
top level: BBa_K292004
start: 1128
end: 1173
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054568
display id: annotation2054568
version: 1
top level: BBa_K292004
location: https://synbiohub.org/public/igem/BBa_K292004/annotation2054568/range2054568/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292004/annotation2054568/range2054568/1
type: Range
persistent identity: range2054568
display id: range2054568
version: 1
direction: direction/forward
top level: BBa_K292004
start: 1
end: 41
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J61000
display id: BBa_J61000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol resistance cassette
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chloramphenicol resistance gene including its native promoter and ribosome binding site. Confers resistance to 25 ug/mL chloramphenicol. {pSB1A2-Bca1016}
mutable notes: N/A
mutable provenance: PCR ca1016F/R on pKD3 (934 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Products are Bca1015 and Bca1016 ---- ca1016F Forward EcoRI to biobrick CAT of pKD3 gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg ca1016R Reverse SpeI to biobrick CAT of pKD3 ccgctactagtggcgcgcctacctgtgacgg
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_J61000_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1892270
display id: annotation1892270
version: 1
top level: CmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1

https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1
type: Range
persistent identity: range1892270
display id: range1892270
version: 1
direction: direction/reverse
top level: CmR
start: 30
end: 687
orientation: inline

TetR
type: ComponentDefinition
persistent identity: BBa P1001
display id: BBa_P1001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline resistance cassette
creator: Austin Che
created: 2005-12-19T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 22
owning group id: 41
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This cassette provides the entire tetracycline resistance operon and will express TetR.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: tetA promoter
sequence annotation: tetA
sequence: https://synbiohub.org/public/igem/BBa_P1001_sequence/1

tetA promoter
type: SequenceAnnotation
persistent identity: annotation1771322
display id: annotation1771322
version: 1
top level: TetR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1001/annotation1771322/range1771322/1

https://synbiohub.org/public/igem/BBa_P1001/annotation1771322/range1771322/1
type: Range
persistent identity: range1771322
display id: range1771322
version: 1
direction: direction/forward
top level: TetR
start: 1
end: 88
orientation: inline

tetA
type: SequenceAnnotation
persistent identity: annotation1771321
display id: annotation1771321
version: 1
top level: TetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1001/annotation1771321/range1771321/1

https://synbiohub.org/public/igem/BBa_P1001/annotation1771321/range1771321/1
type: Range
persistent identity: range1771321
display id: range1771321
version: 1
direction: direction/forward
top level: TetR
start: 89
end: 1279
orientation: inline

ampR
type: ComponentDefinition
persistent identity: BBa P1002
display id: BBa_P1002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ampicillin resistance cassette
creator: Reshma Shetty
created: 2006-01-23T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ampicillin resistance cassette for modular BioBricks vector construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ampR RBS
sequence annotation: ampR -35
sequence annotation: ampicillin resistance
sequence annotation: ampR -10
sequence: https://synbiohub.org/public/igem/BBa_P1002_sequence/1

ampR RBS
type: SequenceAnnotation
persistent identity: annotation1897111
display id: annotation1897111
version: 1
top level: ampR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897111/range1897111/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1897111/range1897111/1
type: Range
persistent identity: range1897111
display id: range1897111
version: 1
direction: direction/forward
top level: ampR
start: 71
end: 75
orientation: inline

ampR -35
type: SequenceAnnotation
persistent identity: annotation1897109
display id: annotation1897109
version: 1
top level: ampR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897109/range1897109/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1897109/range1897109/1
type: Range
persistent identity: range1897109
display id: range1897109
version: 1
direction: direction/forward
top level: ampR
start: 31
end: 36
orientation: inline

ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation1790786
display id: annotation1790786
version: 1
top level: ampR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1790786/range1790786/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1790786/range1790786/1
type: Range
persistent identity: range1790786
display id: range1790786
version: 1
direction: direction/forward
top level: ampR
start: 80
end: 943
orientation: inline

ampR -10
type: SequenceAnnotation
persistent identity: annotation1897110
display id: annotation1897110
version: 1
top level: ampR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897110/range1897110/1

https://synbiohub.org/public/igem/BBa_P1002/annotation1897110/range1897110/1
type: Range
persistent identity: range1897110
display id: range1897110
version: 1
direction: direction/forward
top level: ampR
start: 57
end: 62
orientation: inline

gmR
type: ComponentDefinition
persistent identity: BBa P1014
display id: BBa_P1014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gentamicin resistance cassette aph(2)
creator: Tom Knight
created: 2006-06-10T11:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155
mutable notes: XbaI site at 660 removed by mutating c->t which also improves codon usage. gatgatttttacagggatattttagagaacgaaatctac,P1014-XF gtagatttcgttctctaaaatatccctgtaaaaatcatc,P1014-XR
mutable provenance: DQ208936
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: stem_loop
sequence annotation: aph(2)
sequence: https://synbiohub.org/public/igem/BBa_P1014_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2073661
display id: annotation2073661
version: 1
top level: gmR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_P1014/annotation2073661/range2073661/1

https://synbiohub.org/public/igem/BBa_P1014/annotation2073661/range2073661/1
type: Range
persistent identity: range2073661
display id: range2073661
version: 1
direction: direction/forward
top level: gmR
start: 1040
end: 1070
orientation: inline

aph(2)
type: SequenceAnnotation
persistent identity: annotation2073660
display id: annotation2073660
version: 1
top level: gmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1014/annotation2073660/range2073660/1

https://synbiohub.org/public/igem/BBa_P1014/annotation2073660/range2073660/1
type: Range
persistent identity: range2073660
display id: range2073660
version: 1
direction: direction/forward
top level: gmR
start: 120
end: 1025
orientation: inline

gmR
type: ComponentDefinition
persistent identity: BBa P1013
display id: BBa_P1013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gentamycin resistance cassette (aacC1 gene)
creator: Tom Knight
created: 2006-03-16T12:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The aacC1 gene conveys resistance to gentamycin only. It is derived from a Tn21 series transposon (Wohlleben89) found on the R1033 plasmid (Pseudomonas aeruginosa). This part is created by PCR from the pJAT8 plasmid (Keastling lab) which in turn is derived from pJN105 (Newman and Fuqua).
mutable notes: pJAT8 plasmid edited with primers: GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ccttgtcgccttgcgtataa,aacC1-F GTT TCT TCC TGC AGC GGC CGC TAC TAG TA atctcggcttgaacgaattg,aacC1-R
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS
sequence annotation: -35 / -10 promoter sites
sequence annotation: aacC1 coding sequence
sequence: https://synbiohub.org/public/igem/BBa_P1013_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1826615
display id: annotation1826615
version: 1
top level: gmR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826615/range1826615/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826615/range1826615/1
type: Range
persistent identity: range1826615
display id: range1826615
version: 1
direction: direction/forward
top level: gmR
start: 276
end: 279
orientation: inline

-35 / -10 promoter sites
type: SequenceAnnotation
persistent identity: annotation1826613
display id: annotation1826613
version: 1
top level: gmR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826613/range1826613/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826613/range1826613/1
type: Range
persistent identity: range1826613
display id: range1826613
version: 1
direction: direction/forward
top level: gmR
start: 71
end: 99
orientation: inline

aacC1 coding sequence
type: SequenceAnnotation
persistent identity: annotation1826614
display id: annotation1826614
version: 1
top level: gmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826614/range1826614/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826614/range1826614/1
type: Range
persistent identity: range1826614
display id: range1826614
version: 1
direction: direction/forward
top level: gmR
start: 288
end: 821
orientation: inline

cmR
type: ComponentDefinition
persistent identity: BBa P1004
display id: BBa_P1004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol resistance cassette
creator: Reshma Shetty
created: 2006-02-03T12:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same part as BBa_P1002 except in reverse orientation.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: cmR
sequence: https://synbiohub.org/public/igem/BBa_P1004_sequence/1

cmR
type: SequenceAnnotation
persistent identity: annotation1897114
display id: annotation1897114
version: 1
top level: cmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1004/annotation1897114/range1897114/1

https://synbiohub.org/public/igem/BBa_P1004/annotation1897114/range1897114/1
type: Range
persistent identity: range1897114
display id: range1897114
version: 1
direction: direction/forward
top level: cmR
start: 107
end: 769
orientation: inline

BBa_K1937002
type: ComponentDefinition
persistent identity: BBa K1937002
display id: BBa_K1937002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriKan: a composite part to turn any pSB1C3 into a Bacillus plasmid
creator: Brice Enjalbert
created: 2016-10-12T11:00:00Z
modified: 2016-10-15T03:11:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2404_
m user id: 21497
owner id: 21497
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Part: BBa_K1937002 (pSB1C3-OriKan) (Chassis E. coli/B. subtilis, carrier plasmid pSB1C3) Length: 2510 bp While Bacillus subtilis is of huge interest for a growing number of iGEM projects, it is not easy to develop new parts as it is required that they registered after sub-cloning in the E. coli plasmid pSB1C3. During the iGEM Toulouse 2016 project, we had the idea to create a part that could turn any pSB1C3-based plasmid in a shuttle vector (E. coli/B. subtilis). This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
mutable notes: We successfully transformed E. coli and selected clones on chloramphenicol. Likewise, we successfully transformed B. subtilis and selected clones on kanamycine. Presence of the pSB1C3-OriKan plasmid was demonstrated in B. subtilis by PCR checking. The part sequence has been verified by sequencing.
mutable provenance: The BBa_K1937002 part contains the repU origin of replication of B. subtilis and the kanamycin resistance gene for B. subtilis. It was obtained by amplifying the repU-Kan region of pSBBsOK_P (BBa_K1351040) using primers OriKan forward (5??? cacagaatcaggggataacgcaggaaagaaACATGTAGTTATAAGTGACTAAACAAATAACTAAATAGATGGG) and OriKan reverse (5??? gttcctggccttttgctggccttttgctcaACATGTCGCAAAATGGCCCGATTTAAG). The resulting fragment was sub-cloned in the pSB1C3 between the EcoRI and PstI restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Kananmycin resistance (Bacillus)
sequence annotation: RepU Bacillus origin
sequence: https://synbiohub.org/public/igem/BBa_K1937002_sequence/1

Kananmycin resistance (Bacillus)
type: SequenceAnnotation
persistent identity: annotation2497330
display id: annotation2497330
version: 1
top level: BBa_K1937002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1937002/annotation2497330/range2497330/1

https://synbiohub.org/public/igem/BBa_K1937002/annotation2497330/range2497330/1
type: Range
persistent identity: range2497330
display id: range2497330
version: 1
direction: direction/forward
top level: BBa_K1937002
start: 1595
end: 2365
orientation: inline

RepU Bacillus origin
type: SequenceAnnotation
persistent identity: annotation2497331
display id: annotation2497331
version: 1
top level: BBa_K1937002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1937002/annotation2497331/range2497331/1

https://synbiohub.org/public/igem/BBa_K1937002/annotation2497331/range2497331/1
type: Range
persistent identity: range2497331
display id: range2497331
version: 1
direction: direction/forward
top level: BBa_K1937002
start: 431
end: 1435
orientation: inline

PcpcB-Kana
type: ComponentDefinition
persistent identity: BBa K1642003
display id: BBa_K1642003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kanamycin Resistance Cassette in Cyanobacteria
creator: Can Yin
created: 2015-08-14T11:00:00Z
modified: 2015-09-18T04:39:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2059_
m user id: 24423
owner id: 24405
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Constitutively
mutable notes: Kana
mutable provenance: 6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: KanR
sequence annotation: PcpcB
sequence: https://synbiohub.org/public/igem/BBa_K1642003_sequence/1

KanR
type: SequenceAnnotation
persistent identity: annotation2435670
display id: annotation2435670
version: 1
top level: PcpcB-Kana
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1642003/annotation2435670/range2435670/1

https://synbiohub.org/public/igem/BBa_K1642003/annotation2435670/range2435670/1
type: Range
persistent identity: range2435670
display id: range2435670
version: 1
direction: direction/forward
top level: PcpcB-Kana
start: 590
end: 1402
orientation: inline

PcpcB
type: SequenceAnnotation
persistent identity: annotation2435669
display id: annotation2435669
version: 1
top level: PcpcB-Kana
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1642003/annotation2435669/range2435669/1

https://synbiohub.org/public/igem/BBa_K1642003/annotation2435669/range2435669/1
type: Range
persistent identity: range2435669
display id: range2435669
version: 1
direction: direction/forward
top level: PcpcB-Kana
start: 1
end: 589
orientation: inline