//plasmidbackbone/copynumber/inducible
type: Collection
persistent identity: plasmidbackbone copynumber inducible
display id: plasmidbackbone_copynumber_inducible
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K879918
member: BBa_K879917
member: pSB2K3
member: pSB2K4

BBa_K879917
type: ComponentDefinition
persistent identity: BBa K879917
display id: BBa_K879917
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacO ori
creator: Thomas Patrick
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: lacO ori
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VF2
sequence annotation: BioBrick prefix
sequence annotation: Chloramphenicol resistance
sequence annotation: -35
sequence annotation: RNA II
sequence annotation: pMB1 derived replication origin
sequence annotation: T0 terminator
sequence annotation: -10
sequence annotation: BioBrick suffix
sequence annotation: VR
sequence annotation: E coli his operon terminator
sequence annotation: LacO operator
sequence: https://synbiohub.org/public/igem/BBa_K879917_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation2210382
display id: annotation2210382
version: 1
top level: BBa_K879917
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210382/range2210382/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210382/range2210382/1
type: Range
persistent identity: range2210382
display id: range2210382
version: 1
direction: direction/forward
top level: BBa_K879917
start: 1935
end: 1954
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2210342
display id: annotation2210342
version: 1
top level: BBa_K879917
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210342/range2210342/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210342/range2210342/1
type: Range
persistent identity: range2210342
display id: range2210342
version: 1
direction: direction/forward
top level: BBa_K879917
start: 2053
end: 2072
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2210380
display id: annotation2210380
version: 1
top level: BBa_K879917
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210380/range2210380/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210380/range2210380/1
type: Range
persistent identity: range2210380
display id: range2210380
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 1162
end: 1821
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2210568
display id: annotation2210568
version: 1
top level: BBa_K879917
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210568/range2210568/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210568/range2210568/1
type: Range
persistent identity: range2210568
display id: range2210568
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 859
end: 864
orientation: inline

RNA II
type: SequenceAnnotation
persistent identity: annotation2210383
display id: annotation2210383
version: 1
top level: BBa_K879917
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210383/range2210383/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210383/range2210383/1
type: Range
persistent identity: range2210383
display id: range2210383
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 277
end: 826
orientation: inline

pMB1 derived replication origin
type: SequenceAnnotation
persistent identity: annotation2210378
display id: annotation2210378
version: 1
top level: BBa_K879917
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210378/range2210378/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210378/range2210378/1
type: Range
persistent identity: range2210378
display id: range2210378
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 263
end: 877
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2210379
display id: annotation2210379
version: 1
top level: BBa_K879917
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210379/range2210379/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210379/range2210379/1
type: Range
persistent identity: range2210379
display id: range2210379
version: 1
direction: direction/forward
top level: BBa_K879917
start: 1044
end: 1149
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2210566
display id: annotation2210566
version: 1
top level: BBa_K879917
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210566/range2210566/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210566/range2210566/1
type: Range
persistent identity: range2210566
display id: range2210566
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 836
end: 841
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2210341
display id: annotation2210341
version: 1
top level: BBa_K879917
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210341/range2210341/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210341/range2210341/1
type: Range
persistent identity: range2210341
display id: range2210341
version: 1
direction: direction/forward
top level: BBa_K879917
start: 1
end: 21
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2210377
display id: annotation2210377
version: 1
top level: BBa_K879917
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210377/range2210377/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210377/range2210377/1
type: Range
persistent identity: range2210377
display id: range2210377
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 159
end: 178
orientation: inline

E coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2210376
display id: annotation2210376
version: 1
top level: BBa_K879917
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210376/range2210376/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210376/range2210376/1
type: Range
persistent identity: range2210376
display id: range2210376
version: 1
direction: direction/forward
top level: BBa_K879917
start: 22
end: 95
orientation: inline

LacO operator
type: SequenceAnnotation
persistent identity: annotation2210567
display id: annotation2210567
version: 1
top level: BBa_K879917
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210567/range2210567/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210567/range2210567/1
type: Range
persistent identity: range2210567
display id: range2210567
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 842
end: 858
orientation: inline

pSB2K4
type: ComponentDefinition
persistent identity: pSB2K4
display id: pSB2K4
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB2K3-derived BioBrick plasmid free of many restriction sites
creator: Austin Che, Leon Chan and Sri Kosuri
created: 2006-01-19T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 22
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Derived from pSB2K3 with many restriction enzyme sites removed by site-directed mutagenesis (~2 dozen mutations). Plasmid is useful when there is a need to cut with a variety of different restriction enzymes. Most symmetric cutters have been removed, and some non-symmetric cutters have also including AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, and SgrAI. Works in exactly same way as pSB2K3. See <bb_part>pSB2K3</bb_part> for more information. Note that the VF2 site has a single base mutation in the middle of it. However, VF2 has been used successfully as a primer for PCR with this plasmid.
mutable provenance: pSB2K3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Stem loop
sequence annotation: nnpp promoter 3 (0.95)'
sequence annotation: T->G
sequence annotation: T->A
sequence annotation: f1 ori
sequence annotation: T->C
sequence annotation: Low GC region
sequence annotation: repE binding site
sequence annotation: Maybe A or G
sequence annotation: VF2 (not an exact match, but works)
sequence annotation: Predicted transcription start
sequence annotation: G->T
sequence annotation: lacOP
sequence annotation: BioBrick prefix
sequence annotation: kanR
sequence annotation: incC
sequence annotation: Verification reverse
sequence annotation: Predicted -35
sequence annotation: nnpp promoter 2 (0.96)'
sequence annotation: BioBrick suffix
sequence annotation: oriS
sequence annotation: Possible -35
sequence annotation: Possible -10
sequence annotation: E. coli his operon terminator
sequence annotation: repL
sequence annotation: G->A
sequence annotation: C->T
sequence annotation: G->C
sequence annotation: nnpp promoter 1 (.92)'
sequence annotation: Predicted -10
sequence annotation: end of terminator

Stem loop
type: SequenceAnnotation
persistent identity: annotation1974293
display id: annotation1974293
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974293/range1974293/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974293/range1974293/1
type: Range
persistent identity: range1974293
display id: range1974293
version: 1
direction: direction/forward
top level: pSB2K4
start: 59
end: 91
orientation: inline

nnpp promoter 3 (0.95)'
type: SequenceAnnotation
persistent identity: annotation1974339
display id: annotation1974339
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974339/range1974339/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974339/range1974339/1
type: Range
persistent identity: range1974339
display id: range1974339
version: 1
direction: direction/forward
top level: pSB2K4
start: 3269
end: 3314
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974334
display id: annotation1974334
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974334/range1974334/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974334/range1974334/1
type: Range
persistent identity: range1974334
display id: range1974334
version: 1
direction: direction/forward
top level: pSB2K4
start: 2699
end: 2699
orientation: inline

T->A
type: SequenceAnnotation
persistent identity: annotation1974345
display id: annotation1974345
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974345/range1974345/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974345/range1974345/1
type: Range
persistent identity: range1974345
display id: range1974345
version: 1
direction: direction/forward
top level: pSB2K4
start: 3507
end: 3507
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation1974297
display id: annotation1974297
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974297/range1974297/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974297/range1974297/1
type: Range
persistent identity: range1974297
display id: range1974297
version: 1
direction: direction/reverse
top level: pSB2K4
start: 269
end: 317
orientation: inline

T->A
type: SequenceAnnotation
persistent identity: annotation1974298
display id: annotation1974298
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974298/range1974298/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974298/range1974298/1
type: Range
persistent identity: range1974298
display id: range1974298
version: 1
direction: direction/forward
top level: pSB2K4
start: 292
end: 292
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974338
display id: annotation1974338
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974338/range1974338/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974338/range1974338/1
type: Range
persistent identity: range1974338
display id: range1974338
version: 1
direction: direction/forward
top level: pSB2K4
start: 3251
end: 3251
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974349
display id: annotation1974349
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974349/range1974349/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974349/range1974349/1
type: Range
persistent identity: range1974349
display id: range1974349
version: 1
direction: direction/forward
top level: pSB2K4
start: 3903
end: 3903
orientation: inline

Low GC region
type: SequenceAnnotation
persistent identity: annotation1974329
display id: annotation1974329
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974329/range1974329/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974329/range1974329/1
type: Range
persistent identity: range1974329
display id: range1974329
version: 1
direction: direction/forward
top level: pSB2K4
start: 1636
end: 1682
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974324
display id: annotation1974324
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974324/range1974324/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974324/range1974324/1
type: Range
persistent identity: range1974324
display id: range1974324
version: 1
direction: direction/forward
top level: pSB2K4
start: 1497
end: 1515
orientation: inline

Maybe A or G
type: SequenceAnnotation
persistent identity: annotation1974332
display id: annotation1974332
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974332/range1974332/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974332/range1974332/1
type: Range
persistent identity: range1974332
display id: range1974332
version: 1
direction: direction/forward
top level: pSB2K4
start: 2565
end: 2565
orientation: inline

Low GC region
type: SequenceAnnotation
persistent identity: annotation1974330
display id: annotation1974330
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974330/range1974330/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974330/range1974330/1
type: Range
persistent identity: range1974330
display id: range1974330
version: 1
direction: direction/forward
top level: pSB2K4
start: 1823
end: 1844
orientation: inline

VF2 (not an exact match, but works)
type: SequenceAnnotation
persistent identity: annotation1974351
display id: annotation1974351
version: 1
top level: pSB2K4
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974351/range1974351/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974351/range1974351/1
type: Range
persistent identity: range1974351
display id: range1974351
version: 1
direction: direction/forward
top level: pSB2K4
start: 4313
end: 4332
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974318
display id: annotation1974318
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974318/range1974318/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974318/range1974318/1
type: Range
persistent identity: range1974318
display id: range1974318
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1447
end: 1447
orientation: inline

G->T
type: SequenceAnnotation
persistent identity: annotation1974319
display id: annotation1974319
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974319/range1974319/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974319/range1974319/1
type: Range
persistent identity: range1974319
display id: range1974319
version: 1
direction: direction/forward
top level: pSB2K4
start: 1454
end: 1454
orientation: inline

lacOP
type: SequenceAnnotation
persistent identity: annotation1974331
display id: annotation1974331
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974331/range1974331/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974331/range1974331/1
type: Range
persistent identity: range1974331
display id: range1974331
version: 1
direction: direction/forward
top level: pSB2K4
start: 2064
end: 2238
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1974355
display id: annotation1974355
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974355/range1974355/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974355/range1974355/1
type: Range
persistent identity: range1974355
display id: range1974355
version: 1
direction: direction/forward
top level: pSB2K4
start: 4446
end: 4467
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1974348
display id: annotation1974348
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974348/range1974348/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974348/range1974348/1
type: Range
persistent identity: range1974348
display id: range1974348
version: 1
direction: direction/forward
top level: pSB2K4
start: 3460
end: 4275
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974314
display id: annotation1974314
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974314/range1974314/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974314/range1974314/1
type: Range
persistent identity: range1974314
display id: range1974314
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1426
end: 1426
orientation: inline

incC
type: SequenceAnnotation
persistent identity: annotation1974302
display id: annotation1974302
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974302/range1974302/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974302/range1974302/1
type: Range
persistent identity: range1974302
display id: range1974302
version: 1
direction: direction/reverse
top level: pSB2K4
start: 394
end: 641
orientation: inline

Verification reverse
type: SequenceAnnotation
persistent identity: annotation1974296
display id: annotation1974296
version: 1
top level: pSB2K4
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974296/range1974296/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974296/range1974296/1
type: Range
persistent identity: range1974296
display id: range1974296
version: 1
direction: direction/reverse
top level: pSB2K4
start: 184
end: 203
orientation: inline

Predicted -35
type: SequenceAnnotation
persistent identity: annotation1974340
display id: annotation1974340
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974340/range1974340/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974340/range1974340/1
type: Range
persistent identity: range1974340
display id: range1974340
version: 1
direction: direction/forward
top level: pSB2K4
start: 3359
end: 3364
orientation: inline

nnpp promoter 2 (0.96)'
type: SequenceAnnotation
persistent identity: annotation1974341
display id: annotation1974341
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974341/range1974341/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974341/range1974341/1
type: Range
persistent identity: range1974341
display id: range1974341
version: 1
direction: direction/forward
top level: pSB2K4
start: 3353
end: 3398
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1974354
display id: annotation1974354
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974354/range1974354/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974354/range1974354/1
type: Range
persistent identity: range1974354
display id: range1974354
version: 1
direction: direction/forward
top level: pSB2K4
start: 4402
end: 4421
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1974291
display id: annotation1974291
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974291/range1974291/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974291/range1974291/1
type: Range
persistent identity: range1974291
display id: range1974291
version: 1
direction: direction/forward
top level: pSB2K4
start: 1
end: 21
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974346
display id: annotation1974346
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974346/range1974346/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974346/range1974346/1
type: Range
persistent identity: range1974346
display id: range1974346
version: 1
direction: direction/forward
top level: pSB2K4
start: 3702
end: 3702
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974294
display id: annotation1974294
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974294/range1974294/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974294/range1974294/1
type: Range
persistent identity: range1974294
display id: range1974294
version: 1
direction: direction/forward
top level: pSB2K4
start: 93
end: 93
orientation: inline

oriS
type: SequenceAnnotation
persistent identity: annotation1974328
display id: annotation1974328
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974328/range1974328/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974328/range1974328/1
type: Range
persistent identity: range1974328
display id: range1974328
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1490
end: 1706
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974327
display id: annotation1974327
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974327/range1974327/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974327/range1974327/1
type: Range
persistent identity: range1974327
display id: range1974327
version: 1
direction: direction/forward
top level: pSB2K4
start: 1581
end: 1591
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974305
display id: annotation1974305
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974305/range1974305/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974305/range1974305/1
type: Range
persistent identity: range1974305
display id: range1974305
version: 1
direction: direction/forward
top level: pSB2K4
start: 578
end: 578
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974315
display id: annotation1974315
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974315/range1974315/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974315/range1974315/1
type: Range
persistent identity: range1974315
display id: range1974315
version: 1
direction: direction/forward
top level: pSB2K4
start: 1426
end: 1431
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974336
display id: annotation1974336
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974336/range1974336/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974336/range1974336/1
type: Range
persistent identity: range1974336
display id: range1974336
version: 1
direction: direction/forward
top level: pSB2K4
start: 2774
end: 2774
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974343
display id: annotation1974343
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974343/range1974343/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974343/range1974343/1
type: Range
persistent identity: range1974343
display id: range1974343
version: 1
direction: direction/forward
top level: pSB2K4
start: 3394
end: 3394
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974326
display id: annotation1974326
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974326/range1974326/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974326/range1974326/1
type: Range
persistent identity: range1974326
display id: range1974326
version: 1
direction: direction/forward
top level: pSB2K4
start: 1552
end: 1570
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974317
display id: annotation1974317
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974317/range1974317/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974317/range1974317/1
type: Range
persistent identity: range1974317
display id: range1974317
version: 1
direction: direction/forward
top level: pSB2K4
start: 1444
end: 1452
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974301
display id: annotation1974301
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974301/range1974301/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974301/range1974301/1
type: Range
persistent identity: range1974301
display id: range1974301
version: 1
direction: direction/reverse
top level: pSB2K4
start: 477
end: 495
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974316
display id: annotation1974316
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974316/range1974316/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974316/range1974316/1
type: Range
persistent identity: range1974316
display id: range1974316
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1433
end: 1441
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974299
display id: annotation1974299
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974299/range1974299/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974299/range1974299/1
type: Range
persistent identity: range1974299
display id: range1974299
version: 1
direction: direction/reverse
top level: pSB2K4
start: 394
end: 412
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1974292
display id: annotation1974292
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974292/range1974292/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974292/range1974292/1
type: Range
persistent identity: range1974292
display id: range1974292
version: 1
direction: direction/forward
top level: pSB2K4
start: 51
end: 120
orientation: inline

repL
type: SequenceAnnotation
persistent identity: annotation1974335
display id: annotation1974335
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974335/range1974335/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974335/range1974335/1
type: Range
persistent identity: range1974335
display id: range1974335
version: 1
direction: direction/forward
top level: pSB2K4
start: 2316
end: 3161
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974307
display id: annotation1974307
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974307/range1974307/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974307/range1974307/1
type: Range
persistent identity: range1974307
display id: range1974307
version: 1
direction: direction/reverse
top level: pSB2K4
start: 623
end: 641
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974304
display id: annotation1974304
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974304/range1974304/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974304/range1974304/1
type: Range
persistent identity: range1974304
display id: range1974304
version: 1
direction: direction/reverse
top level: pSB2K4
start: 568
end: 586
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation1974337
display id: annotation1974337
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974337/range1974337/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974337/range1974337/1
type: Range
persistent identity: range1974337
display id: range1974337
version: 1
direction: direction/forward
top level: pSB2K4
start: 3017
end: 3017
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1974313
display id: annotation1974313
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974313/range1974313/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974313/range1974313/1
type: Range
persistent identity: range1974313
display id: range1974313
version: 1
direction: direction/forward
top level: pSB2K4
start: 1142
end: 1142
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974325
display id: annotation1974325
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974325/range1974325/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974325/range1974325/1
type: Range
persistent identity: range1974325
display id: range1974325
version: 1
direction: direction/forward
top level: pSB2K4
start: 1527
end: 1537
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974306
display id: annotation1974306
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974306/range1974306/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974306/range1974306/1
type: Range
persistent identity: range1974306
display id: range1974306
version: 1
direction: direction/reverse
top level: pSB2K4
start: 597
end: 619
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974322
display id: annotation1974322
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974322/range1974322/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974322/range1974322/1
type: Range
persistent identity: range1974322
display id: range1974322
version: 1
direction: direction/forward
top level: pSB2K4
start: 1460
end: 1460
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974347
display id: annotation1974347
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974347/range1974347/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974347/range1974347/1
type: Range
persistent identity: range1974347
display id: range1974347
version: 1
direction: direction/forward
top level: pSB2K4
start: 3849
end: 3849
orientation: inline

nnpp promoter 1 (.92)'
type: SequenceAnnotation
persistent identity: annotation1974344
display id: annotation1974344
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974344/range1974344/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974344/range1974344/1
type: Range
persistent identity: range1974344
display id: range1974344
version: 1
direction: direction/forward
top level: pSB2K4
start: 3375
end: 3420
orientation: inline

Predicted -10
type: SequenceAnnotation
persistent identity: annotation1974342
display id: annotation1974342
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974342/range1974342/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974342/range1974342/1
type: Range
persistent identity: range1974342
display id: range1974342
version: 1
direction: direction/forward
top level: pSB2K4
start: 3379
end: 3387
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1974353
display id: annotation1974353
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974353/range1974353/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974353/range1974353/1
type: Range
persistent identity: range1974353
display id: range1974353
version: 1
direction: direction/forward
top level: pSB2K4
start: 4389
end: 4392
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974323
display id: annotation1974323
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974323/range1974323/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974323/range1974323/1
type: Range
persistent identity: range1974323
display id: range1974323
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1477
end: 1482
orientation: inline

C->A
type: SequenceAnnotation
persistent identity: annotation1974295
display id: annotation1974295
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974295/range1974295/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974295/range1974295/1
type: Range
persistent identity: range1974295
display id: range1974295
version: 1
direction: direction/forward
top level: pSB2K4
start: 146
end: 146
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974311
display id: annotation1974311
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974311/range1974311/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974311/range1974311/1
type: Range
persistent identity: range1974311
display id: range1974311
version: 1
direction: direction/forward
top level: pSB2K4
start: 1091
end: 1091
orientation: inline

A->G mutation
type: SequenceAnnotation
persistent identity: annotation1974300
display id: annotation1974300
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974300/range1974300/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974300/range1974300/1
type: Range
persistent identity: range1974300
display id: range1974300
version: 1
direction: direction/forward
top level: pSB2K4
start: 462
end: 462
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974321
display id: annotation1974321
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974321/range1974321/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974321/range1974321/1
type: Range
persistent identity: range1974321
display id: range1974321
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1455
end: 1460
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974312
display id: annotation1974312
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974312/range1974312/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974312/range1974312/1
type: Range
persistent identity: range1974312
display id: range1974312
version: 1
direction: direction/forward
top level: pSB2K4
start: 1100
end: 1100
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1974310
display id: annotation1974310
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974310/range1974310/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974310/range1974310/1
type: Range
persistent identity: range1974310
display id: range1974310
version: 1
direction: direction/reverse
top level: pSB2K4
start: 644
end: 1399
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974352
display id: annotation1974352
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974352/range1974352/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974352/range1974352/1
type: Range
persistent identity: range1974352
display id: range1974352
version: 1
direction: direction/forward
top level: pSB2K4
start: 4321
end: 4321
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1974350
display id: annotation1974350
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974350/range1974350/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974350/range1974350/1
type: Range
persistent identity: range1974350
display id: range1974350
version: 1
direction: direction/forward
top level: pSB2K4
start: 4119
end: 4119
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974333
display id: annotation1974333
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974333/range1974333/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974333/range1974333/1
type: Range
persistent identity: range1974333
display id: range1974333
version: 1
direction: direction/forward
top level: pSB2K4
start: 2567
end: 2567
orientation: inline

A->C
type: SequenceAnnotation
persistent identity: annotation1974308
display id: annotation1974308
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974308/range1974308/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974308/range1974308/1
type: Range
persistent identity: range1974308
display id: range1974308
version: 1
direction: direction/forward
top level: pSB2K4
start: 692
end: 692
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974309
display id: annotation1974309
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974309/range1974309/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974309/range1974309/1
type: Range
persistent identity: range1974309
display id: range1974309
version: 1
direction: direction/forward
top level: pSB2K4
start: 857
end: 857
orientation: inline

T->A mutation
type: SequenceAnnotation
persistent identity: annotation1974303
display id: annotation1974303
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974303/range1974303/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974303/range1974303/1
type: Range
persistent identity: range1974303
display id: range1974303
version: 1
direction: direction/forward
top level: pSB2K4
start: 551
end: 551
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974320
display id: annotation1974320
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974320/range1974320/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974320/range1974320/1
type: Range
persistent identity: range1974320
display id: range1974320
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1455
end: 1463
orientation: inline

pSB2K3
type: ComponentDefinition
persistent identity: pSB2K3
display id: pSB2K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible copy number BioBrick plasmid
creator: Sri Kosuri
created: 2004-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_48_46_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB2K3 is a variable copy number plasmid carrying kanamycin resistance. The plasmid contains the F' replication origin and also the P1 lytic replication origin. Genes encoding proteins that are required to activate the P1 lytic replication origin are carried on the plasmid under the control of a lacI-regulated promoter. When grown in cells producing LacI the plasmid is maintained at very low copy (<10 per cell). When induced with IPTG the P1 lytic replication proteins are produced and the P1 lytic replication origin takes over, raising the copy number to very high levels (>100++ per cell). The copy control features of pSB2K3 are useful for cloning "difficult" parts while still being able to prepare large amounts of DNA. Additionally, pSB2K3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.
mutable notes: *** pSCANS via BNL *** The pSB2 series plasmids are based on the pSCANS-5 plasmid from John Dunn's group at Brookhaven National Laboratory. The pSCANS plasmids are introduced here.
mutable provenance: MIT SBWG modification of pSCANS-5 from BNL
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: F1 Replication Origin
sequence annotation: end of terminator
sequence annotation: VF2
sequence annotation: nnpp promoters
sequence annotation: terminator stem loop
sequence annotation: prefix
sequence annotation: oriS Replication Origin (rev)
sequence annotation: E. coli his operon terminator
sequence annotation: kanR
sequence annotation: repL
sequence annotation: repE (rev)
sequence annotation: lacOP
sequence annotation: suffix
sequence annotation: deletion of two bases
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/pSB2K3_sequence/1

F1 Replication Origin
type: SequenceAnnotation
persistent identity: annotation1023023
display id: annotation1023023
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023023/range1023023/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023023/range1023023/1
type: Range
persistent identity: range1023023
display id: range1023023
version: 1
direction: direction/forward
top level: pSB2K3
start: 242
end: 290
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824017
display id: annotation1824017
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824017/range1824017/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824017/range1824017/1
type: Range
persistent identity: range1824017
display id: range1824017
version: 1
direction: direction/reverse
top level: pSB2K3
start: 4362
end: 4365
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782484
display id: annotation1782484
version: 1
top level: pSB2K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K3/annotation1782484/range1782484/1

https://synbiohub.org/public/igem/pSB2K3/annotation1782484/range1782484/1
type: Range
persistent identity: range1782484
display id: range1782484
version: 1
direction: direction/forward
top level: pSB2K3
start: 4286
end: 4305
orientation: inline

nnpp promoters
type: SequenceAnnotation
persistent identity: annotation1023028
display id: annotation1023028
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023028/range1023028/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023028/range1023028/1
type: Range
persistent identity: range1023028
display id: range1023028
version: 1
direction: direction/forward
top level: pSB2K3
start: 3242
end: 3393
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824014
display id: annotation1824014
version: 1
top level: pSB2K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824014/range1824014/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824014/range1824014/1
type: Range
persistent identity: range1824014
display id: range1824014
version: 1
direction: direction/forward
top level: pSB2K3
start: 30
end: 64
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1023021
display id: annotation1023021
version: 1
top level: pSB2K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023021/range1023021/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023021/range1023021/1
type: Range
persistent identity: range1023021
display id: range1023021
version: 1
direction: direction/forward
top level: pSB2K3
start: 4404
end: 4425
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824016
display id: annotation1824016
version: 1
top level: pSB2K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824016/range1824016/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824016/range1824016/1
type: Range
persistent identity: range1824016
display id: range1824016
version: 1
direction: direction/reverse
top level: pSB2K3
start: 4375
end: 4394
orientation: inline

oriS Replication Origin (rev)
type: SequenceAnnotation
persistent identity: annotation1023025
display id: annotation1023025
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023025/range1023025/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023025/range1023025/1
type: Range
persistent identity: range1023025
display id: range1023025
version: 1
direction: direction/reverse
top level: pSB2K3
start: 1463
end: 1679
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824015
display id: annotation1824015
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824015/range1824015/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824015/range1824015/1
type: Range
persistent identity: range1824015
display id: range1824015
version: 1
direction: direction/forward
top level: pSB2K3
start: 22
end: 93
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1023020
display id: annotation1023020
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023020/range1023020/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023020/range1023020/1
type: Range
persistent identity: range1023020
display id: range1023020
version: 1
direction: direction/forward
top level: pSB2K3
start: 3433
end: 4248
orientation: inline

repL
type: SequenceAnnotation
persistent identity: annotation1023027
display id: annotation1023027
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023027/range1023027/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023027/range1023027/1
type: Range
persistent identity: range1023027
display id: range1023027
version: 1
direction: direction/forward
top level: pSB2K3
start: 2289
end: 3134
orientation: inline

repE (rev)
type: SequenceAnnotation
persistent identity: annotation1023024
display id: annotation1023024
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023024/range1023024/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023024/range1023024/1
type: Range
persistent identity: range1023024
display id: range1023024
version: 1
direction: direction/reverse
top level: pSB2K3
start: 617
end: 1372
orientation: inline

lacOP
type: SequenceAnnotation
persistent identity: annotation1023026
display id: annotation1023026
version: 1
top level: pSB2K3
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023026/range1023026/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023026/range1023026/1
type: Range
persistent identity: range1023026
display id: range1023026
version: 1
direction: direction/forward
top level: pSB2K3
start: 2037
end: 2211
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1023022
display id: annotation1023022
version: 1
top level: pSB2K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023022/range1023022/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023022/range1023022/1
type: Range
persistent identity: range1023022
display id: range1023022
version: 1
direction: direction/forward
top level: pSB2K3
start: 1
end: 21
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823781
display id: annotation1823781
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1823781/range1823781/1

https://synbiohub.org/public/igem/pSB2K3/annotation1823781/range1823781/1
type: Range
persistent identity: range1823781
display id: range1823781
version: 1
direction: direction/forward
top level: pSB2K3
start: 34
end: 35
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782483
display id: annotation1782483
version: 1
top level: pSB2K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K3/annotation1782483/range1782483/1

https://synbiohub.org/public/igem/pSB2K3/annotation1782483/range1782483/1
type: Range
persistent identity: range1782483
display id: range1782483
version: 1
direction: direction/reverse
top level: pSB2K3
start: 157
end: 176
orientation: inline

BBa_K879918
type: ComponentDefinition
persistent identity: BBa K879918
display id: BBa_K879918
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetO ori
creator: Nien-Tsu Tseng
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: TetO ori
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: TetO operator
sequence annotation: E coli his operon terminator
sequence annotation: Chloramphenicol resistance
sequence annotation: -35
sequence annotation: BioBrick suffix
sequence annotation: pMB1 derived replication origin
sequence annotation: BioBrick prefix
sequence annotation: RNA II
sequence annotation: VF2
sequence annotation: T0 terminator
sequence annotation: -10
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_K879918_sequence/1

TetO operator
type: SequenceAnnotation
persistent identity: annotation2210755
display id: annotation2210755
version: 1
top level: BBa_K879918
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210755/range2210755/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210755/range2210755/1
type: Range
persistent identity: range2210755
display id: range2210755
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 842
end: 858
orientation: inline

E coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2210750
display id: annotation2210750
version: 1
top level: BBa_K879918
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210750/range2210750/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210750/range2210750/1
type: Range
persistent identity: range2210750
display id: range2210750
version: 1
direction: direction/forward
top level: BBa_K879918
start: 22
end: 95
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2210758
display id: annotation2210758
version: 1
top level: BBa_K879918
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210758/range2210758/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210758/range2210758/1
type: Range
persistent identity: range2210758
display id: range2210758
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 1162
end: 1821
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2210756
display id: annotation2210756
version: 1
top level: BBa_K879918
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210756/range2210756/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210756/range2210756/1
type: Range
persistent identity: range2210756
display id: range2210756
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 859
end: 864
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2210749
display id: annotation2210749
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210749/range2210749/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210749/range2210749/1
type: Range
persistent identity: range2210749
display id: range2210749
version: 1
direction: direction/forward
top level: BBa_K879918
start: 1
end: 21
orientation: inline

pMB1 derived replication origin
type: SequenceAnnotation
persistent identity: annotation2210752
display id: annotation2210752
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210752/range2210752/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210752/range2210752/1
type: Range
persistent identity: range2210752
display id: range2210752
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 263
end: 877
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2210775
display id: annotation2210775
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210775/range2210775/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210775/range2210775/1
type: Range
persistent identity: range2210775
display id: range2210775
version: 1
direction: direction/forward
top level: BBa_K879918
start: 2053
end: 2072
orientation: inline

RNA II
type: SequenceAnnotation
persistent identity: annotation2210753
display id: annotation2210753
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210753/range2210753/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210753/range2210753/1
type: Range
persistent identity: range2210753
display id: range2210753
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 277
end: 826
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2210759
display id: annotation2210759
version: 1
top level: BBa_K879918
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210759/range2210759/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210759/range2210759/1
type: Range
persistent identity: range2210759
display id: range2210759
version: 1
direction: direction/forward
top level: BBa_K879918
start: 1935
end: 1954
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2210757
display id: annotation2210757
version: 1
top level: BBa_K879918
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210757/range2210757/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210757/range2210757/1
type: Range
persistent identity: range2210757
display id: range2210757
version: 1
direction: direction/forward
top level: BBa_K879918
start: 1044
end: 1149
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2210754
display id: annotation2210754
version: 1
top level: BBa_K879918
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210754/range2210754/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210754/range2210754/1
type: Range
persistent identity: range2210754
display id: range2210754
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 836
end: 841
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2210751
display id: annotation2210751
version: 1
top level: BBa_K879918
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210751/range2210751/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210751/range2210751/1
type: Range
persistent identity: range2210751
display id: range2210751
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 159
end: 178
orientation: inline