//plasmidbackbone/copynumber/low
type: Collection
persistent identity: plasmidbackbone copynumber low
display id: plasmidbackbone_copynumber_low
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K864013
member: pSB8K15
member: BBa_K864000
member: lcp
member: pSB4C1
member: pSB8C15FRT
member: pSB4A5
member: BBa_K864005
member: BAC
member: BBa_K864014
member: BBa_K864012
member: BBa_K864006
member: pSB8A15FRT
member: pSB8S15
member: pSB4K50
member: BBa_K1680014
member: BBa_K864015
member: pSB4K5
member: pSB4T5
member: pSB4C50
member: BBa_K864011
member: BBa_K1680015
member: BBa_K864002
member: BBa_K864007
member: BBa_K864010
member: pSB3K1
member: pSB4A3
member: pSB4A50
member: BBa_K864003
member: BBa_K864008
member: B. S. Vect
member: pSB8A15
member: pSB8S15FRT
member: BBa_K1680016
member: BBa_K864001
member: pSB4C5
member: pSB8C15
member: BBa_K125000
member: BBa_K864004
member: BBa_K864009
member: pSB8K15FRT

BBa_K864001
type: ComponentDefinition
persistent identity: BBa K864001
display id: BBa_K864001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4C15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Tandem repeat
sequence annotation: CmR BBa_P1004
sequence annotation: rrnC terminator
sequence annotation: P22 late terminator
sequence annotation: -35
sequence annotation: T3 terminator
sequence annotation: repA
sequence annotation: VR
sequence annotation: Lux terminator
sequence annotation: pSC101 ori
sequence annotation: VF2
sequence annotation: Lambda Red forward primer
sequence annotation: Mutated A>T to remove SpeI
sequence annotation: Promoter
sequence annotation: Lambda Red reverse primer
sequence annotation: -10
sequence annotation: tonB terminator
sequence annotation: Repeat "4"
sequence annotation: Repeat "5"
sequence: https://synbiohub.org/public/igem/BBa_K864001_sequence/1

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198576
display id: annotation2198576
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198576/range2198576/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198576/range2198576/1
type: Range
persistent identity: range2198576
display id: range2198576
version: 1
direction: direction/forward
top level: BBa_K864001
start: 790
end: 809
orientation: inline

CmR BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2198620
display id: annotation2198620
version: 1
top level: BBa_K864001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198620/range2198620/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198620/range2198620/1
type: Range
persistent identity: range2198620
display id: range2198620
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 2506
end: 3168
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198566
display id: annotation2198566
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198566/range2198566/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198566/range2198566/1
type: Range
persistent identity: range2198566
display id: range2198566
version: 1
direction: direction/forward
top level: BBa_K864001
start: 136
end: 175
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198628
display id: annotation2198628
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198628/range2198628/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198628/range2198628/1
type: Range
persistent identity: range2198628
display id: range2198628
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3251
end: 3321
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198584
display id: annotation2198584
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198584/range2198584/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198584/range2198584/1
type: Range
persistent identity: range2198584
display id: range2198584
version: 1
direction: direction/forward
top level: BBa_K864001
start: 881
end: 886
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198614
display id: annotation2198614
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198614/range2198614/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198614/range2198614/1
type: Range
persistent identity: range2198614
display id: range2198614
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3342
end: 3419
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2198605
display id: annotation2198605
version: 1
top level: BBa_K864001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198605/range2198605/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198605/range2198605/1
type: Range
persistent identity: range2198605
display id: range2198605
version: 1
direction: direction/forward
top level: BBa_K864001
start: 939
end: 1889
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198565
display id: annotation2198565
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198565/range2198565/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198565/range2198565/1
type: Range
persistent identity: range2198565
display id: range2198565
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 116
end: 135
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198616
display id: annotation2198616
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198616/range2198616/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198616/range2198616/1
type: Range
persistent identity: range2198616
display id: range2198616
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 2454
end: 2499
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2198568
display id: annotation2198568
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198568/range2198568/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198568/range2198568/1
type: Range
persistent identity: range2198568
display id: range2198568
version: 1
direction: direction/forward
top level: BBa_K864001
start: 190
end: 2405
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198629
display id: annotation2198629
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198629/range2198629/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198629/range2198629/1
type: Range
persistent identity: range2198629
display id: range2198629
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3322
end: 3341
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198578
display id: annotation2198578
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198578/range2198578/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198578/range2198578/1
type: Range
persistent identity: range2198578
display id: range2198578
version: 1
direction: direction/forward
top level: BBa_K864001
start: 820
end: 841
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198606
display id: annotation2198606
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198606/range2198606/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198606/range2198606/1
type: Range
persistent identity: range2198606
display id: range2198606
version: 1
direction: direction/forward
top level: BBa_K864001
start: 2433
end: 2450
orientation: inline

Mutated A>T to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2198582
display id: annotation2198582
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198582/range2198582/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198582/range2198582/1
type: Range
persistent identity: range2198582
display id: range2198582
version: 1
direction: direction/forward
top level: BBa_K864001
start: 867
end: 867
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198574
display id: annotation2198574
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198574/range2198574/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198574/range2198574/1
type: Range
persistent identity: range2198574
display id: range2198574
version: 1
direction: direction/forward
top level: BBa_K864001
start: 767
end: 788
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2198622
display id: annotation2198622
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198622/range2198622/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198622/range2198622/1
type: Range
persistent identity: range2198622
display id: range2198622
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 3235
end: 3261
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198340
display id: annotation2198340
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198340/range2198340/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198340/range2198340/1
type: Range
persistent identity: range2198340
display id: range2198340
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 18
end: 38
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198603
display id: annotation2198603
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198603/range2198603/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198603/range2198603/1
type: Range
persistent identity: range2198603
display id: range2198603
version: 1
direction: direction/forward
top level: BBa_K864001
start: 903
end: 908
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2198561
display id: annotation2198561
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198561/range2198561/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198561/range2198561/1
type: Range
persistent identity: range2198561
display id: range2198561
version: 1
direction: direction/forward
top level: BBa_K864001
start: 47
end: 115
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2198580
display id: annotation2198580
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198580/range2198580/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198580/range2198580/1
type: Range
persistent identity: range2198580
display id: range2198580
version: 1
direction: direction/forward
top level: BBa_K864001
start: 862
end: 880
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2198585
display id: annotation2198585
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198585/range2198585/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198585/range2198585/1
type: Range
persistent identity: range2198585
display id: range2198585
version: 1
direction: direction/forward
top level: BBa_K864001
start: 891
end: 912
orientation: inline

BBa_K864004
type: ComponentDefinition
persistent identity: BBa K864004
display id: BBa_K864004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Lux terminator
sequence annotation: VF2
sequence annotation: -10
sequence annotation: Lambda Red forward primer
sequence annotation: TonB terminator
sequence annotation: P22 late terminator
sequence annotation: T3 terminator
sequence annotation: rrnC terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: RBS
sequence annotation: VR
sequence annotation: AmpR P1002
sequence annotation: FRT site
sequence annotation: -35
sequence annotation: pSC101 ori BBa_K864050
sequence: https://synbiohub.org/public/igem/BBa_K864004_sequence/1

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198871
display id: annotation2198871
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198871/range2198871/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198871/range2198871/1
type: Range
persistent identity: range2198871
display id: range2198871
version: 1
direction: direction/forward
top level: BBa_K864004
start: 2454
end: 2499
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198873
display id: annotation2198873
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198873/range2198873/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198873/range2198873/1
type: Range
persistent identity: range2198873
display id: range2198873
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3564
end: 3583
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198877
display id: annotation2198877
version: 1
top level: BBa_K864004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198877/range2198877/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198877/range2198877/1
type: Range
persistent identity: range2198877
display id: range2198877
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3421
end: 3426
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198870
display id: annotation2198870
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198870/range2198870/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198870/range2198870/1
type: Range
persistent identity: range2198870
display id: range2198870
version: 1
direction: direction/forward
top level: BBa_K864004
start: 2433
end: 2450
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2198866
display id: annotation2198866
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198866/range2198866/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198866/range2198866/1
type: Range
persistent identity: range2198866
display id: range2198866
version: 1
direction: direction/forward
top level: BBa_K864004
start: 47
end: 115
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198872
display id: annotation2198872
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198872/range2198872/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198872/range2198872/1
type: Range
persistent identity: range2198872
display id: range2198872
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3523
end: 3563
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198874
display id: annotation2198874
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198874/range2198874/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198874/range2198874/1
type: Range
persistent identity: range2198874
display id: range2198874
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3584
end: 3661
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198868
display id: annotation2198868
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198868/range2198868/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198868/range2198868/1
type: Range
persistent identity: range2198868
display id: range2198868
version: 1
direction: direction/forward
top level: BBa_K864004
start: 136
end: 175
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198865
display id: annotation2198865
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198865/range2198865/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198865/range2198865/1
type: Range
persistent identity: range2198865
display id: range2198865
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 18
end: 38
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2198876
display id: annotation2198876
version: 1
top level: BBa_K864004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198876/range2198876/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198876/range2198876/1
type: Range
persistent identity: range2198876
display id: range2198876
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3408
end: 3412
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198867
display id: annotation2198867
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198867/range2198867/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198867/range2198867/1
type: Range
persistent identity: range2198867
display id: range2198867
version: 1
direction: direction/forward
top level: BBa_K864004
start: 116
end: 135
orientation: inline

AmpR P1002
type: SequenceAnnotation
persistent identity: annotation2198875
display id: annotation2198875
version: 1
top level: BBa_K864004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198875/range2198875/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198875/range2198875/1
type: Range
persistent identity: range2198875
display id: range2198875
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 2543
end: 3403
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2198880
display id: annotation2198880
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198880/range2198880/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198880/range2198880/1
type: Range
persistent identity: range2198880
display id: range2198880
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3483
end: 3516
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2198879
display id: annotation2198879
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198879/range2198879/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198879/range2198879/1
type: Range
persistent identity: range2198879
display id: range2198879
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 2506
end: 2539
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198878
display id: annotation2198878
version: 1
top level: BBa_K864004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198878/range2198878/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198878/range2198878/1
type: Range
persistent identity: range2198878
display id: range2198878
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3447
end: 3452
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2198869
display id: annotation2198869
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198869/range2198869/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198869/range2198869/1
type: Range
persistent identity: range2198869
display id: range2198869
version: 1
direction: direction/forward
top level: BBa_K864004
start: 190
end: 2405
orientation: inline

BBa_K864006
type: ComponentDefinition
persistent identity: BBa K864006
display id: BBa_K864006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864006_sequence/1

BBa_K125000
type: ComponentDefinition
persistent identity: BBa K125000
display id: BBa_K125000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Broad host range BioBrick plasmid derived from pRL1383a with low to medium copy number
creator: Grace Kwan
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3229
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RSF1010 is a broad-host-range plasmid first described in 1974 (Guerry 1974), with its entire sequence and gene organization subsequently described in 1989 (Scholz 1989). It is a naturally occurring 8.6kb broad-host-range plasmid in the E. coli incompatibility group Q. The conjugative transfer and stable replication of this plasmid are possible due to the mob genes with the associated origin of transfer (oriT) and the rep genes with the associated origin of vegetative replication (oriV), respectively. RSF1010 derived plasmids which include the oriV and associated Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, and Serratia (Leemans 1987). In addition, RSF1010 derived plasmids including the oriV, oriT and its associated rep and mob genes are transferred by conjugation to at least four cyanobacteria strains (Mermet-Bouvier 1993). These cyanobacteria strains include Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301. A number of RSF1010 derived plasmids have been constructed due to the utility of this broad-host-range plasmid. For example, pSB2A, containing a 5.6kb RSF1010 derived region including the necessary mobilization and replication regions, can be transferred through conjugation to and stably maintained in Synechocystis PCC6803, PCC6714, and Synechococcus PCC7942 and PCC6301 (Marraccini 1993). The RSF1010 plasmid used in this study, pRL1383a was constructed for use in genomic studies of the diazotrophic, multicellular cyanobacterium Anabaena PCC 7120. This plasmid contains the mob and rep regions necessary for conjugation and autonomous replication, respectively. Additionally this vector is made resistant to Streptomycin and Spectinomycin due to the presence of the aadA gene (Wolk 2007). This plasmid can be mobilized by the E. coli derived self-transmissible plasmid RP4. Mobilization genes are not necessary for transfer of a mobilizable plasmid if the self-transmissible pl…
mutable notes: The original pRL1383a MCS has been replaced by the BioBrick BBa_B0034 and its flanking BioBrick restriction sites.
mutable provenance: Plasmid is derived from pRL1383a.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick suffix
sequence annotation: cosN
sequence annotation: T4 32 terminator
sequence annotation: P5 promoter
sequence annotation: RSF1010 derived region
sequence annotation: oriR
sequence annotation: VR
sequence annotation: loxP
sequence annotation: T7 promoter
sequence annotation: omega interposon
sequence annotation: VF2
sequence annotation: oriT
sequence annotation: BioBrick prefix
sequence annotation: spectinomycin marker
sequence: https://synbiohub.org/public/igem/BBa_K125000_sequence/1

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1999638
display id: annotation1999638
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999638/range1999638/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999638/range1999638/1
type: Range
persistent identity: range1999638
display id: range1999638
version: 1
direction: direction/forward
top level: BBa_K125000
start: 1
end: 21
orientation: inline

cosN
type: SequenceAnnotation
persistent identity: annotation1999634
display id: annotation1999634
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999634/range1999634/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999634/range1999634/1
type: Range
persistent identity: range1999634
display id: range1999634
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8616
end: 8705
orientation: inline

T4 32 terminator
type: SequenceAnnotation
persistent identity: annotation1999636
display id: annotation1999636
version: 1
top level: BBa_K125000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999636/range1999636/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999636/range1999636/1
type: Range
persistent identity: range1999636
display id: range1999636
version: 1
direction: direction/forward
top level: BBa_K125000
start: 343
end: 374
orientation: inline

P5 promoter
type: SequenceAnnotation
persistent identity: annotation1969227
display id: annotation1969227
version: 1
top level: BBa_K125000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969227/range1969227/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969227/range1969227/1
type: Range
persistent identity: range1969227
display id: range1969227
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8200
end: 8230
orientation: inline

RSF1010 derived region
type: SequenceAnnotation
persistent identity: annotation1969582
display id: annotation1969582
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969582/range1969582/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969582/range1969582/1
type: Range
persistent identity: range1969582
display id: range1969582
version: 1
direction: direction/forward
top level: BBa_K125000
start: 2664
end: 8356
orientation: inline

oriR
type: SequenceAnnotation
persistent identity: annotation1969581
display id: annotation1969581
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969581/range1969581/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969581/range1969581/1
type: Range
persistent identity: range1969581
display id: range1969581
version: 1
direction: direction/forward
top level: BBa_K125000
start: 2819
end: 3243
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1969228
display id: annotation1969228
version: 1
top level: BBa_K125000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969228/range1969228/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969228/range1969228/1
type: Range
persistent identity: range1969228
display id: range1969228
version: 1
direction: direction/reverse
top level: BBa_K125000
start: 78
end: 97
orientation: inline

T4 32 terminator
type: SequenceAnnotation
persistent identity: annotation1999637
display id: annotation1999637
version: 1
top level: BBa_K125000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999637/range1999637/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999637/range1999637/1
type: Range
persistent identity: range1999637
display id: range1999637
version: 1
direction: direction/forward
top level: BBa_K125000
start: 2249
end: 2280
orientation: inline

loxP
type: SequenceAnnotation
persistent identity: annotation1999635
display id: annotation1999635
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999635/range1999635/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999635/range1999635/1
type: Range
persistent identity: range1999635
display id: range1999635
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8831
end: 8864
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999633
display id: annotation1999633
version: 1
top level: BBa_K125000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999633/range1999633/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999633/range1999633/1
type: Range
persistent identity: range1999633
display id: range1999633
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8880
end: 8898
orientation: inline

omega interposon
type: SequenceAnnotation
persistent identity: annotation1969583
display id: annotation1969583
version: 1
top level: BBa_K125000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969583/range1969583/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969583/range1969583/1
type: Range
persistent identity: range1969583
display id: range1969583
version: 1
direction: direction/forward
top level: BBa_K125000
start: 463
end: 1255
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1969229
display id: annotation1969229
version: 1
top level: BBa_K125000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969229/range1969229/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969229/range1969229/1
type: Range
persistent identity: range1969229
display id: range1969229
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8908
end: 8927
orientation: inline

oriT
type: SequenceAnnotation
persistent identity: annotation1969230
display id: annotation1969230
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969230/range1969230/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969230/range1969230/1
type: Range
persistent identity: range1969230
display id: range1969230
version: 1
direction: direction/forward
top level: BBa_K125000
start: 3476
end: 3633
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1999639
display id: annotation1999639
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999639/range1999639/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999639/range1999639/1
type: Range
persistent identity: range1999639
display id: range1999639
version: 1
direction: direction/forward
top level: BBa_K125000
start: 9026
end: 9047
orientation: inline

spectinomycin marker
type: SequenceAnnotation
persistent identity: annotation1996995
display id: annotation1996995
version: 1
top level: BBa_K125000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1996995/range1996995/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1996995/range1996995/1
type: Range
persistent identity: range1996995
display id: range1996995
version: 1
direction: direction/forward
top level: BBa_K125000
start: 933
end: 1943
orientation: inline

pSB8A15FRT
type: ComponentDefinition
persistent identity: BBa K864020
display id: BBa_K864020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864020_sequence/1

pSB3K1
type: ComponentDefinition
persistent identity: pSB3K1
display id: pSB3K1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pACYC177 converted into BioBrick vector (formerly BBa_I739202)
creator: Stefan Luzi
created: 2007-10-22T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: partial Tn903 repressor fragment
sequence annotation: Tn903 inverted repeat
sequence annotation: BioBrick suffix
sequence annotation: Tn3 inverted repeat
sequence annotation: partial ampicillin resistance marker (nonfunctional)
sequence annotation: p15A replication origin
sequence annotation: BioBrick prefix
sequence annotation: kanamycin resistance marker
sequence: https://synbiohub.org/public/igem/pSB3K1_sequence/1

partial Tn903 repressor fragment
type: SequenceAnnotation
persistent identity: annotation1969628
display id: annotation1969628
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969628/range1969628/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969628/range1969628/1
type: Range
persistent identity: range1969628
display id: range1969628
version: 1
direction: direction/forward
top level: pSB3K1
start: 2742
end: 3036
orientation: inline

Tn903 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969626
display id: annotation1969626
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969626/range1969626/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969626/range1969626/1
type: Range
persistent identity: range1969626
display id: range1969626
version: 1
direction: direction/forward
top level: pSB3K1
start: 1307
end: 1534
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969629
display id: annotation1969629
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969629/range1969629/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969629/range1969629/1
type: Range
persistent identity: range1969629
display id: range1969629
version: 1
direction: direction/forward
top level: pSB3K1
start: 1
end: 21
orientation: inline

Tn3 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969625
display id: annotation1969625
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969625/range1969625/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969625/range1969625/1
type: Range
persistent identity: range1969625
display id: range1969625
version: 1
direction: direction/forward
top level: pSB3K1
start: 445
end: 482
orientation: inline

partial ampicillin resistance marker (nonfunctional)
type: SequenceAnnotation
persistent identity: annotation1969624
display id: annotation1969624
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969624/range1969624/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969624/range1969624/1
type: Range
persistent identity: range1969624
display id: range1969624
version: 1
direction: direction/forward
top level: pSB3K1
start: 22
end: 332
orientation: inline

p15A replication origin
type: SequenceAnnotation
persistent identity: annotation1969622
display id: annotation1969622
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969622/range1969622/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969622/range1969622/1
type: Range
persistent identity: range1969622
display id: range1969622
version: 1
direction: direction/forward
top level: pSB3K1
start: 483
end: 1312
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969630
display id: annotation1969630
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969630/range1969630/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969630/range1969630/1
type: Range
persistent identity: range1969630
display id: range1969630
version: 1
direction: direction/forward
top level: pSB3K1
start: 3043
end: 3064
orientation: inline

Tn903 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969627
display id: annotation1969627
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969627/range1969627/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969627/range1969627/1
type: Range
persistent identity: range1969627
display id: range1969627
version: 1
direction: direction/forward
top level: pSB3K1
start: 2515
end: 2742
orientation: inline

kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation1969623
display id: annotation1969623
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969623/range1969623/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969623/range1969623/1
type: Range
persistent identity: range1969623
display id: range1969623
version: 1
direction: direction/forward
top level: pSB3K1
start: 1637
end: 2453
orientation: inline

BBa_K864000
type: ComponentDefinition
persistent identity: BBa K864000
display id: BBa_K864000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ??? antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RBS
sequence annotation: tonB terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: VF2
sequence annotation: Tandem repeat
sequence annotation: -35
sequence annotation: Lux terminator
sequence annotation: -10
sequence annotation: rrnC terminator
sequence annotation: ampR BBa_P1002
sequence annotation: Mutated A>T to remove SpeI
sequence annotation: repA
sequence annotation: Lambda Red forward primer
sequence annotation: pSC101 ori
sequence annotation: VR
sequence annotation: T3 terminator
sequence annotation: Repeat "5"
sequence annotation: Repeat "4"
sequence: https://synbiohub.org/public/igem/BBa_K864000_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2198034
display id: annotation2198034
version: 1
top level: BBa_K864000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198034/range2198034/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198034/range2198034/1
type: Range
persistent identity: range2198034
display id: range2198034
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3374
end: 3378
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2198074
display id: annotation2198074
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198074/range2198074/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198074/range2198074/1
type: Range
persistent identity: range2198074
display id: range2198074
version: 1
direction: direction/forward
top level: BBa_K864000
start: 47
end: 115
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198031
display id: annotation2198031
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198031/range2198031/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198031/range2198031/1
type: Range
persistent identity: range2198031
display id: range2198031
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 18
end: 38
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198032
display id: annotation2198032
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198032/range2198032/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198032/range2198032/1
type: Range
persistent identity: range2198032
display id: range2198032
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3495
end: 3514
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198039
display id: annotation2198039
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198039/range2198039/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198039/range2198039/1
type: Range
persistent identity: range2198039
display id: range2198039
version: 1
direction: direction/forward
top level: BBa_K864000
start: 820
end: 841
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198010
display id: annotation2198010
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198010/range2198010/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198010/range2198010/1
type: Range
persistent identity: range2198010
display id: range2198010
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 3413
end: 3418
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198075
display id: annotation2198075
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198075/range2198075/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198075/range2198075/1
type: Range
persistent identity: range2198075
display id: range2198075
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 2454
end: 2499
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198007
display id: annotation2198007
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198007/range2198007/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198007/range2198007/1
type: Range
persistent identity: range2198007
display id: range2198007
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 3387
end: 3392
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198093
display id: annotation2198093
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198093/range2198093/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198093/range2198093/1
type: Range
persistent identity: range2198093
display id: range2198093
version: 1
direction: direction/forward
top level: BBa_K864000
start: 136
end: 175
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198009
display id: annotation2198009
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198009/range2198009/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198009/range2198009/1
type: Range
persistent identity: range2198009
display id: range2198009
version: 1
direction: direction/forward
top level: BBa_K864000
start: 881
end: 886
orientation: inline

ampR BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2198012
display id: annotation2198012
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198012/range2198012/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198012/range2198012/1
type: Range
persistent identity: range2198012
display id: range2198012
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 2509
end: 3369
orientation: inline

Mutated A>T to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2198029
display id: annotation2198029
version: 1
top level: BBa_K864000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198029/range2198029/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198029/range2198029/1
type: Range
persistent identity: range2198029
display id: range2198029
version: 1
direction: direction/forward
top level: BBa_K864000
start: 867
end: 867
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2198011
display id: annotation2198011
version: 1
top level: BBa_K864000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198011/range2198011/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198011/range2198011/1
type: Range
persistent identity: range2198011
display id: range2198011
version: 1
direction: direction/forward
top level: BBa_K864000
start: 939
end: 1889
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198006
display id: annotation2198006
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198006/range2198006/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198006/range2198006/1
type: Range
persistent identity: range2198006
display id: range2198006
version: 1
direction: direction/forward
top level: BBa_K864000
start: 903
end: 908
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198037
display id: annotation2198037
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198037/range2198037/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198037/range2198037/1
type: Range
persistent identity: range2198037
display id: range2198037
version: 1
direction: direction/forward
top level: BBa_K864000
start: 767
end: 788
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198030
display id: annotation2198030
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198030/range2198030/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198030/range2198030/1
type: Range
persistent identity: range2198030
display id: range2198030
version: 1
direction: direction/forward
top level: BBa_K864000
start: 2433
end: 2450
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198036
display id: annotation2198036
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198036/range2198036/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198036/range2198036/1
type: Range
persistent identity: range2198036
display id: range2198036
version: 1
direction: direction/forward
top level: BBa_K864000
start: 790
end: 809
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2198057
display id: annotation2198057
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198057/range2198057/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198057/range2198057/1
type: Range
persistent identity: range2198057
display id: range2198057
version: 1
direction: direction/forward
top level: BBa_K864000
start: 190
end: 2405
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198033
display id: annotation2198033
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198033/range2198033/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198033/range2198033/1
type: Range
persistent identity: range2198033
display id: range2198033
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 116
end: 135
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198076
display id: annotation2198076
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198076/range2198076/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198076/range2198076/1
type: Range
persistent identity: range2198076
display id: range2198076
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3515
end: 3592
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2198056
display id: annotation2198056
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198056/range2198056/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198056/range2198056/1
type: Range
persistent identity: range2198056
display id: range2198056
version: 1
direction: direction/forward
top level: BBa_K864000
start: 891
end: 912
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2198038
display id: annotation2198038
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198038/range2198038/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198038/range2198038/1
type: Range
persistent identity: range2198038
display id: range2198038
version: 1
direction: direction/forward
top level: BBa_K864000
start: 862
end: 880
orientation: inline

BBa_K864005
type: ComponentDefinition
persistent identity: BBa K864005
display id: BBa_K864005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. The resistance cassette is flanked by FRT sites (BBa_J61020) which allows for subsequent removal of the resistance cassette from the chromosome by the FLP recombinase. The pSB8x15 series are especially suitable for carrying the FLP recombinase, since they can be removed by incubation at 42??C.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR
sequence annotation: Lux terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: TonB terminator
sequence annotation: Lambda Red forward primer
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: rrnC terminator
sequence: https://synbiohub.org/public/igem/BBa_K864005_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation2200046
display id: annotation2200046
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200046/range2200046/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200046/range2200046/1
type: Range
persistent identity: range2200046
display id: range2200046
version: 1
direction: direction/reverse
top level: BBa_K864005
start: 116
end: 135
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2200051
display id: annotation2200051
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200051/range2200051/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200051/range2200051/1
type: Range
persistent identity: range2200051
display id: range2200051
version: 1
direction: direction/forward
top level: BBa_K864005
start: 2454
end: 2499
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2200041
display id: annotation2200041
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200041/range2200041/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200041/range2200041/1
type: Range
persistent identity: range2200041
display id: range2200041
version: 1
direction: direction/reverse
top level: BBa_K864005
start: 18
end: 38
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2200045
display id: annotation2200045
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200045/range2200045/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200045/range2200045/1
type: Range
persistent identity: range2200045
display id: range2200045
version: 1
direction: direction/forward
top level: BBa_K864005
start: 47
end: 115
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2200050
display id: annotation2200050
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200050/range2200050/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200050/range2200050/1
type: Range
persistent identity: range2200050
display id: range2200050
version: 1
direction: direction/forward
top level: BBa_K864005
start: 2433
end: 2450
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2200048
display id: annotation2200048
version: 1
top level: BBa_K864005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200048/range2200048/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200048/range2200048/1
type: Range
persistent identity: range2200048
display id: range2200048
version: 1
direction: direction/forward
top level: BBa_K864005
start: 190
end: 2405
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2200047
display id: annotation2200047
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200047/range2200047/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200047/range2200047/1
type: Range
persistent identity: range2200047
display id: range2200047
version: 1
direction: direction/forward
top level: BBa_K864005
start: 136
end: 175
orientation: inline

pSB8C15FRT
type: ComponentDefinition
persistent identity: BBa K864021
display id: BBa_K864021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864021_sequence/1

BBa_K864008
type: ComponentDefinition
persistent identity: BBa K864008
display id: BBa_K864008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864008_sequence/1

lcp
type: ComponentDefinition
persistent identity: BBa I716101
display id: BBa_I716101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low Copy Ampicillin Resistance Plasmid
creator: David Tulga
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _110_
m user id: 0
owner id: 1694
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a Low Copy BioBrick 2.0 Plasmid with the Ampicillin Resistance Gene ampR.
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: This is modified and converted from the BioBrick 1.0 Plasmid pSB4A3.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Berkeley prefix
sequence annotation: RBS
sequence annotation: inverted repeat
sequence annotation: beta-lactamase promoter
sequence annotation: direct repeat
sequence annotation: AT rich region
sequence annotation: par region
sequence annotation: putative polyA
sequence annotation: -10
sequence annotation: -35
sequence annotation: Berkeley suffix
sequence annotation: repA
sequence annotation: promoter region
sequence annotation: VF2 primer binding site
sequence annotation: beta-lactamase ampicillin resistance gene
sequence: https://synbiohub.org/public/igem/BBa_I716101_sequence/1

Berkeley prefix
type: SequenceAnnotation
persistent identity: annotation1974357
display id: annotation1974357
version: 1
top level: lcp
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974357/range1974357/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974357/range1974357/1
type: Range
persistent identity: range1974357
display id: range1974357
version: 1
direction: direction/forward
top level: lcp
start: 3151
end: 3165
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1974379
display id: annotation1974379
version: 1
top level: lcp
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974379/range1974379/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974379/range1974379/1
type: Range
persistent identity: range1974379
display id: range1974379
version: 1
direction: direction/reverse
top level: lcp
start: 2918
end: 2922
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1974364
display id: annotation1974364
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974364/range1974364/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974364/range1974364/1
type: Range
persistent identity: range1974364
display id: range1974364
version: 1
direction: direction/reverse
top level: lcp
start: 1838
end: 1861
orientation: inline

beta-lactamase promoter
type: SequenceAnnotation
persistent identity: annotation1974359
display id: annotation1974359
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974359/range1974359/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974359/range1974359/1
type: Range
persistent identity: range1974359
display id: range1974359
version: 1
direction: direction/reverse
top level: lcp
start: 2955
end: 2983
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1974368
display id: annotation1974368
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974368/range1974368/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974368/range1974368/1
type: Range
persistent identity: range1974368
display id: range1974368
version: 1
direction: direction/reverse
top level: lcp
start: 1297
end: 1316
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation1974366
display id: annotation1974366
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974366/range1974366/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974366/range1974366/1
type: Range
persistent identity: range1974366
display id: range1974366
version: 1
direction: direction/reverse
top level: lcp
start: 1336
end: 1418
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1974362
display id: annotation1974362
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974362/range1974362/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974362/range1974362/1
type: Range
persistent identity: range1974362
display id: range1974362
version: 1
direction: direction/reverse
top level: lcp
start: 1901
end: 1914
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation1974361
display id: annotation1974361
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974361/range1974361/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974361/range1974361/1
type: Range
persistent identity: range1974361
display id: range1974361
version: 1
direction: direction/reverse
top level: lcp
start: 1549
end: 1916
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1974375
display id: annotation1974375
version: 1
top level: lcp
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974375/range1974375/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974375/range1974375/1
type: Range
persistent identity: range1974375
display id: range1974375
version: 1
direction: direction/reverse
top level: lcp
start: 1169
end: 1174
orientation: inline

putative polyA
type: SequenceAnnotation
persistent identity: annotation1974376
display id: annotation1974376
version: 1
top level: lcp
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974376/range1974376/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974376/range1974376/1
type: Range
persistent identity: range1974376
display id: range1974376
version: 1
direction: direction/reverse
top level: lcp
start: 91
end: 106
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1974367
display id: annotation1974367
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974367/range1974367/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974367/range1974367/1
type: Range
persistent identity: range1974367
display id: range1974367
version: 1
direction: direction/reverse
top level: lcp
start: 1318
end: 1337
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1974365
display id: annotation1974365
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974365/range1974365/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974365/range1974365/1
type: Range
persistent identity: range1974365
display id: range1974365
version: 1
direction: direction/reverse
top level: lcp
start: 1815
end: 1832
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1974370
display id: annotation1974370
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974370/range1974370/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974370/range1974370/1
type: Range
persistent identity: range1974370
display id: range1974370
version: 1
direction: direction/reverse
top level: lcp
start: 1194
end: 1215
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1974378
display id: annotation1974378
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974378/range1974378/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974378/range1974378/1
type: Range
persistent identity: range1974378
display id: range1974378
version: 1
direction: direction/reverse
top level: lcp
start: 2931
end: 2936
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1974377
display id: annotation1974377
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974377/range1974377/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974377/range1974377/1
type: Range
persistent identity: range1974377
display id: range1974377
version: 1
direction: direction/reverse
top level: lcp
start: 2957
end: 2962
orientation: inline

Berkeley suffix
type: SequenceAnnotation
persistent identity: annotation1974356
display id: annotation1974356
version: 1
top level: lcp
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974356/range1974356/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974356/range1974356/1
type: Range
persistent identity: range1974356
display id: range1974356
version: 1
direction: direction/forward
top level: lcp
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1974374
display id: annotation1974374
version: 1
top level: lcp
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974374/range1974374/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974374/range1974374/1
type: Range
persistent identity: range1974374
display id: range1974374
version: 1
direction: direction/reverse
top level: lcp
start: 1171
end: 1183
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation1974360
display id: annotation1974360
version: 1
top level: lcp
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974360/range1974360/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974360/range1974360/1
type: Range
persistent identity: range1974360
display id: range1974360
version: 1
direction: direction/reverse
top level: lcp
start: 217
end: 1167
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation1974369
display id: annotation1974369
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974369/range1974369/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974369/range1974369/1
type: Range
persistent identity: range1974369
display id: range1974369
version: 1
direction: direction/reverse
top level: lcp
start: 1265
end: 1284
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation1974373
display id: annotation1974373
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974373/range1974373/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974373/range1974373/1
type: Range
persistent identity: range1974373
display id: range1974373
version: 1
direction: direction/reverse
top level: lcp
start: 1188
end: 1207
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation1974363
display id: annotation1974363
version: 1
top level: lcp
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974363/range1974363/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974363/range1974363/1
type: Range
persistent identity: range1974363
display id: range1974363
version: 1
direction: direction/reverse
top level: lcp
start: 1872
end: 1888
orientation: inline

VF2 primer binding site
type: SequenceAnnotation
persistent identity: annotation1974380
display id: annotation1974380
version: 1
top level: lcp
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974380/range1974380/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974380/range1974380/1
type: Range
persistent identity: range1974380
display id: range1974380
version: 1
direction: direction/forward
top level: lcp
start: 3033
end: 3052
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1974372
display id: annotation1974372
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974372/range1974372/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974372/range1974372/1
type: Range
persistent identity: range1974372
display id: range1974372
version: 1
direction: direction/reverse
top level: lcp
start: 1198
end: 1203
orientation: inline

beta-lactamase ampicillin resistance gene
type: SequenceAnnotation
persistent identity: annotation1974358
display id: annotation1974358
version: 1
top level: lcp
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974358/range1974358/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974358/range1974358/1
type: Range
persistent identity: range1974358
display id: range1974358
version: 1
direction: direction/reverse
top level: lcp
start: 2053
end: 2913
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1974371
display id: annotation1974371
version: 1
top level: lcp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I716101/annotation1974371/range1974371/1

https://synbiohub.org/public/igem/BBa_I716101/annotation1974371/range1974371/1
type: Range
persistent identity: range1974371
display id: range1974371
version: 1
direction: direction/reverse
top level: lcp
start: 1220
end: 1225
orientation: inline

BBa_K864013
type: ComponentDefinition
persistent identity: BBa K864013
display id: BBa_K864013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4C15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864013_sequence/1

pSB8K15FRT
type: ComponentDefinition
persistent identity: BBa K864022
display id: BBa_K864022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: awd
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864022_sequence/1

pSB4C50
type: ComponentDefinition
persistent identity: BBa K1362096
display id: BBa_K1362096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4C50: Low(?) copy BioBrick cloning/expression backbone carrying Cm resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0053
component: BBa_B0042
component: BBa_G0002
component: BBa_B0044
component: BBa_B0062
component: BBa_G0003
component: BBa_G00000
component: BBa_B0043
component: BBa_B0055
component: BBa_G0004
component: BBa_G00102
component: BBa_G00001
component: BBa_I50042
component: BBa_G00100
component: BBa_P1004
component: BBa_B0054
component: BBa_K1362463
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_G00102
sequence annotation: BBa_G00100
sequence annotation: BBa_G0003
sequence annotation: BBa_B0042
sequence annotation: BBa_B0055
sequence annotation: BBa_B0054
sequence annotation: BBa_I50042
sequence annotation: BBa_G00001
sequence annotation: BBa_G0004
sequence annotation: BBa_B0062
sequence annotation: BBa_G0002
sequence annotation: BBa_K1362463
sequence annotation: BBa_P1004
sequence annotation: BBa_G00000
sequence annotation: BBa_B0043
sequence: https://synbiohub.org/public/igem/BBa_K1362096_sequence/1

BBa_B0053
type: Component
persistent identity: component2409906
display id: component2409906
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0042
type: Component
persistent identity: component2409864
display id: component2409864
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: Component
persistent identity: component2409901
display id: component2409901
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0044
type: Component
persistent identity: component2409857
display id: component2409857
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409871
display id: component2409871
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0003
type: Component
persistent identity: component2409872
display id: component2409872
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00000
type: Component
persistent identity: component2409932
display id: component2409932
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0043
type: Component
persistent identity: component2409923
display id: component2409923
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: Component
persistent identity: component2409911
display id: component2409911
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component2409918
display id: component2409918
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0004
type: Component
persistent identity: component2409904
display id: component2409904
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00102
type: Component
persistent identity: component2409869
display id: component2409869
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G00001
type: Component
persistent identity: component2409852
display id: component2409852
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_I50042
type: Component
persistent identity: component2409900
display id: component2409900
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_G00100
type: Component
persistent identity: component2409908
display id: component2409908
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_P1004
type: Component
persistent identity: component2409903
display id: component2409903
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0054
type: Component
persistent identity: component2409867
display id: component2409867
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_K1362463
type: Component
persistent identity: component2409927
display id: component2409927
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409906
display id: annotation2409906
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
type: Range
persistent identity: range2409906
display id: range2409906
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3093
end: 3164
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409857
display id: annotation2409857
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
type: Range
persistent identity: range2409857
display id: range2409857
version: 1
direction: direction/reverse
top level: pSB4C50
start: 30
end: 42
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409869
display id: annotation2409869
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
type: Range
persistent identity: range2409869
display id: range2409869
version: 1
direction: direction/reverse
top level: pSB4C50
start: 148
end: 167
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409908
display id: annotation2409908
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
type: Range
persistent identity: range2409908
display id: range2409908
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3173
end: 3192
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409872
display id: annotation2409872
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
type: Range
persistent identity: range2409872
display id: range2409872
version: 1
direction: direction/reverse
top level: pSB4C50
start: 225
end: 231
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409864
display id: annotation2409864
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
type: Range
persistent identity: range2409864
display id: range2409864
version: 1
direction: direction/reverse
top level: pSB4C50
start: 51
end: 62
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409911
display id: annotation2409911
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
type: Range
persistent identity: range2409911
display id: range2409911
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3201
end: 3278
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409867
display id: annotation2409867
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
type: Range
persistent identity: range2409867
display id: range2409867
version: 1
direction: direction/reverse
top level: pSB4C50
start: 71
end: 139
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409900
display id: annotation2409900
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
type: Range
persistent identity: range2409900
display id: range2409900
version: 1
direction: direction/reverse
top level: pSB4C50
start: 240
end: 2276
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409852
display id: annotation2409852
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
type: Range
persistent identity: range2409852
display id: range2409852
version: 1
direction: direction/reverse
top level: pSB4C50
start: 1
end: 21
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409918
display id: annotation2409918
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
type: Range
persistent identity: range2409918
display id: range2409918
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3287
end: 3298
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409904
display id: annotation2409904
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
type: Range
persistent identity: range2409904
display id: range2409904
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3078
end: 3084
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409871
display id: annotation2409871
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
type: Range
persistent identity: range2409871
display id: range2409871
version: 1
direction: direction/reverse
top level: pSB4C50
start: 176
end: 216
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409901
display id: annotation2409901
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
type: Range
persistent identity: range2409901
display id: range2409901
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2285
end: 2292
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409927
display id: annotation2409927
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
type: Range
persistent identity: range2409927
display id: range2409927
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3328
end: 3378
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2409903
display id: annotation2409903
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
component: BBa_P1004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
type: Range
persistent identity: range2409903
display id: range2409903
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2301
end: 3069
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409932
display id: annotation2409932
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
type: Range
persistent identity: range2409932
display id: range2409932
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3387
end: 3408
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409923
display id: annotation2409923
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
type: Range
persistent identity: range2409923
display id: range2409923
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3307
end: 3319
orientation: inline

BBa_K864011
type: ComponentDefinition
persistent identity: BBa K864011
display id: BBa_K864011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864011_sequence/1

pSB4C5
type: ComponentDefinition
persistent identity: pSB4C5
display id: pSB4C5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-26T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4C3 is a low copy plasmid based with chloramphenicol resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) pSB4C5 is derived from the BioBricks vector scaffold BBa_I51001. However, the direction of the resistance marker and replication origin is not optimal.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, pSC101 based origin and a version of the chloramphenicol rsistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0062
component: BBa_B0042
component: BBa_B0054
component: BBa_G00102
component: BBa_P1004
component: BBa_B0053
component: BBa_G00100
component: BBa_B0055
component: BBa_B0043
component: BBa_I50042
component: BBa_G00001
component: BBa_B0044
component: BBa_G0004
component: BBa_G0003
component: BBa_G00000
component: BBa_G0002
sequence annotation: BBa_G0002
sequence annotation: BBa_I50042
sequence annotation: BBa_B0043
sequence annotation: BBa_B0054
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_G00100
sequence annotation: BBa_G0003
sequence annotation: BBa_G0004
sequence annotation: BBa_G00001
sequence annotation: BBa_G00000
sequence annotation: BBa_P1004
sequence annotation: BBa_B0062
sequence annotation: BBa_G00102
sequence annotation: BBa_B0055
sequence: https://synbiohub.org/public/igem/pSB4C5_sequence/1

BBa_B0062
type: Component
persistent identity: component2236255
display id: component2236255
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
type: Component
persistent identity: component2236248
display id: component2236248
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0054
type: Component
persistent identity: component2236251
display id: component2236251
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00102
type: Component
persistent identity: component2236253
display id: component2236253
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_P1004
type: Component
persistent identity: component2236287
display id: component2236287
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0053
type: Component
persistent identity: component2236290
display id: component2236290
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G00100
type: Component
persistent identity: component2236292
display id: component2236292
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0055
type: Component
persistent identity: component2236295
display id: component2236295
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0043
type: Component
persistent identity: component2236307
display id: component2236307
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_I50042
type: Component
persistent identity: component2236284
display id: component2236284
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_G00001
type: Component
persistent identity: component2236236
display id: component2236236
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0044
type: Component
persistent identity: component2236241
display id: component2236241
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G0004
type: Component
persistent identity: component2236288
display id: component2236288
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G0003
type: Component
persistent identity: component2236256
display id: component2236256
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00000
type: Component
persistent identity: component2236312
display id: component2236312
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G0002
type: Component
persistent identity: component2236285
display id: component2236285
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0042
type: Component
persistent identity: component2236302
display id: component2236302
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236285
display id: annotation2236285
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236285/range2236285/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4C5/annotation2236285/range2236285/1
type: Range
persistent identity: range2236285
display id: range2236285
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2221
end: 2228
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236284
display id: annotation2236284
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236284/range2236284/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4C5/annotation2236284/range2236284/1
type: Range
persistent identity: range2236284
display id: range2236284
version: 1
direction: direction/reverse
top level: pSB4C5
start: 184
end: 2220
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236307
display id: annotation2236307
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236307/range2236307/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4C5/annotation2236307/range2236307/1
type: Range
persistent identity: range2236307
display id: range2236307
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3187
end: 3199
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236251
display id: annotation2236251
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236251/range2236251/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4C5/annotation2236251/range2236251/1
type: Range
persistent identity: range2236251
display id: range2236251
version: 1
direction: direction/reverse
top level: pSB4C5
start: 47
end: 115
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236290
display id: annotation2236290
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236290/range2236290/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4C5/annotation2236290/range2236290/1
type: Range
persistent identity: range2236290
display id: range2236290
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3005
end: 3076
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236241
display id: annotation2236241
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236241/range2236241/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4C5/annotation2236241/range2236241/1
type: Range
persistent identity: range2236241
display id: range2236241
version: 1
direction: direction/reverse
top level: pSB4C5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236248
display id: annotation2236248
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236248/range2236248/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4C5/annotation2236248/range2236248/1
type: Range
persistent identity: range2236248
display id: range2236248
version: 1
direction: direction/reverse
top level: pSB4C5
start: 35
end: 46
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236292
display id: annotation2236292
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236292/range2236292/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4C5/annotation2236292/range2236292/1
type: Range
persistent identity: range2236292
display id: range2236292
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3077
end: 3096
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236256
display id: annotation2236256
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236256/range2236256/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4C5/annotation2236256/range2236256/1
type: Range
persistent identity: range2236256
display id: range2236256
version: 1
direction: direction/reverse
top level: pSB4C5
start: 177
end: 183
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236288
display id: annotation2236288
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236288/range2236288/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4C5/annotation2236288/range2236288/1
type: Range
persistent identity: range2236288
display id: range2236288
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2998
end: 3004
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236236
display id: annotation2236236
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236236/range2236236/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4C5/annotation2236236/range2236236/1
type: Range
persistent identity: range2236236
display id: range2236236
version: 1
direction: direction/reverse
top level: pSB4C5
start: 1
end: 21
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236312
display id: annotation2236312
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236312/range2236312/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4C5/annotation2236312/range2236312/1
type: Range
persistent identity: range2236312
display id: range2236312
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3200
end: 3221
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2236287
display id: annotation2236287
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236287/range2236287/1
component: BBa_P1004

https://synbiohub.org/public/igem/pSB4C5/annotation2236287/range2236287/1
type: Range
persistent identity: range2236287
display id: range2236287
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2229
end: 2997
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236255
display id: annotation2236255
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236255/range2236255/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4C5/annotation2236255/range2236255/1
type: Range
persistent identity: range2236255
display id: range2236255
version: 1
direction: direction/reverse
top level: pSB4C5
start: 136
end: 176
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236253
display id: annotation2236253
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236253/range2236253/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4C5/annotation2236253/range2236253/1
type: Range
persistent identity: range2236253
display id: range2236253
version: 1
direction: direction/reverse
top level: pSB4C5
start: 116
end: 135
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236302
display id: annotation2236302
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236302/range2236302/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4C5/annotation2236302/range2236302/1
type: Range
persistent identity: range2236302
display id: range2236302
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3175
end: 3186
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236295
display id: annotation2236295
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236295/range2236295/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4C5/annotation2236295/range2236295/1
type: Range
persistent identity: range2236295
display id: range2236295
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3097
end: 3174
orientation: inline

BBa_K864007
type: ComponentDefinition
persistent identity: BBa K864007
display id: BBa_K864007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4S15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864007_sequence/1

pSB8C15
type: ComponentDefinition
persistent identity: BBa K864017
display id: BBa_K864017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8C15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and Chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: cmR
sequence annotation: tonB terminator
sequence annotation: T3 terminator
sequence annotation: -10
sequence annotation: VF2 forward primer binding site
sequence annotation: VR reverse primer binding site
sequence annotation: Promoter
sequence annotation: -35
sequence annotation: Lux terminator
sequence annotation: P22 late terminator
sequence annotation: pSC101-ts ori
sequence annotation: rrnC
sequence: https://synbiohub.org/public/igem/BBa_K864017_sequence/1

cmR
type: SequenceAnnotation
persistent identity: annotation2203155
display id: annotation2203155
version: 1
top level: pSB8C15
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203155/range2203155/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203155/range2203155/1
type: Range
persistent identity: range2203155
display id: range2203155
version: 1
direction: direction/reverse
top level: pSB8C15
start: 2506
end: 3168
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2203157
display id: annotation2203157
version: 1
top level: pSB8C15
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203157/range2203157/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203157/range2203157/1
type: Range
persistent identity: range2203157
display id: range2203157
version: 1
direction: direction/forward
top level: pSB8C15
start: 47
end: 115
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2203161
display id: annotation2203161
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203161/range2203161/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203161/range2203161/1
type: Range
persistent identity: range2203161
display id: range2203161
version: 1
direction: direction/forward
top level: pSB8C15
start: 3342
end: 3419
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2203162
display id: annotation2203162
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203162/range2203162/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203162/range2203162/1
type: Range
persistent identity: range2203162
display id: range2203162
version: 1
direction: direction/forward
top level: pSB8C15
start: 903
end: 908
orientation: inline

VF2 forward primer binding site
type: SequenceAnnotation
persistent identity: annotation2203154
display id: annotation2203154
version: 1
top level: pSB8C15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203154/range2203154/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203154/range2203154/1
type: Range
persistent identity: range2203154
display id: range2203154
version: 1
direction: direction/forward
top level: pSB8C15
start: 3322
end: 3341
orientation: inline

VR reverse primer binding site
type: SequenceAnnotation
persistent identity: annotation2203046
display id: annotation2203046
version: 1
top level: pSB8C15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203046/range2203046/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203046/range2203046/1
type: Range
persistent identity: range2203046
display id: range2203046
version: 1
direction: direction/reverse
top level: pSB8C15
start: 116
end: 135
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2202888
display id: annotation2202888
version: 1
top level: pSB8C15
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2202888/range2202888/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2202888/range2202888/1
type: Range
persistent identity: range2202888
display id: range2202888
version: 1
direction: direction/reverse
top level: pSB8C15
start: 3235
end: 3461
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2203156
display id: annotation2203156
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203156/range2203156/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203156/range2203156/1
type: Range
persistent identity: range2203156
display id: range2203156
version: 1
direction: direction/forward
top level: pSB8C15
start: 881
end: 886
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2203159
display id: annotation2203159
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203159/range2203159/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203159/range2203159/1
type: Range
persistent identity: range2203159
display id: range2203159
version: 1
direction: direction/reverse
top level: pSB8C15
start: 2454
end: 2499
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2203160
display id: annotation2203160
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203160/range2203160/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203160/range2203160/1
type: Range
persistent identity: range2203160
display id: range2203160
version: 1
direction: direction/forward
top level: pSB8C15
start: 3281
end: 3321
orientation: inline

pSC101-ts ori
type: SequenceAnnotation
persistent identity: annotation2203153
display id: annotation2203153
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203153/range2203153/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203153/range2203153/1
type: Range
persistent identity: range2203153
display id: range2203153
version: 1
direction: direction/forward
top level: pSB8C15
start: 190
end: 2405
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation2203158
display id: annotation2203158
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203158/range2203158/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203158/range2203158/1
type: Range
persistent identity: range2203158
display id: range2203158
version: 1
direction: direction/forward
top level: pSB8C15
start: 136
end: 175
orientation: inline

pSB8S15
type: ComponentDefinition
persistent identity: BBa K864019
display id: BBa_K864019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8S15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864019_sequence/1

pSB4A50
type: ComponentDefinition
persistent identity: BBa K1362095
display id: BBa_K1362095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A50: Low copy BioBrick cloning/expression backbone carrying Amp resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00100
component: BBa_G0004
component: BBa_G00001
component: BBa_P1002
component: BBa_I50042
component: BBa_B0053
component: BBa_G0003
component: BBa_G00000
component: BBa_B0054
component: BBa_B0042
component: BBa_G0002
component: BBa_B0055
component: BBa_B0044
component: BBa_B0062
component: BBa_K1362463
component: BBa_G00102
component: BBa_B0043
sequence annotation: BBa_G00100
sequence annotation: BBa_P1002
sequence annotation: BBa_B0042
sequence annotation: BBa_I50042
sequence annotation: BBa_B0054
sequence annotation: BBa_B0055
sequence annotation: BBa_G00102
sequence annotation: BBa_G00000
sequence annotation: BBa_B0044
sequence annotation: BBa_B0043
sequence annotation: BBa_B0053
sequence annotation: BBa_G0002
sequence annotation: BBa_G00001
sequence annotation: BBa_G0004
sequence annotation: BBa_K1362463
sequence annotation: BBa_G0003
sequence annotation: BBa_B0062
sequence: https://synbiohub.org/public/igem/BBa_K1362095_sequence/1

BBa_G00100
type: Component
persistent identity: component2409823
display id: component2409823
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G0004
type: Component
persistent identity: component2409819
display id: component2409819
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00001
type: Component
persistent identity: component2409764
display id: component2409764
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_P1002
type: Component
persistent identity: component2409818
display id: component2409818
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_I50042
type: Component
persistent identity: component2409812
display id: component2409812
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0053
type: Component
persistent identity: component2409821
display id: component2409821
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0003
type: Component
persistent identity: component2409784
display id: component2409784
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00000
type: Component
persistent identity: component2409847
display id: component2409847
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0054
type: Component
persistent identity: component2409779
display id: component2409779
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0042
type: Component
persistent identity: component2409776
display id: component2409776
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: Component
persistent identity: component2409813
display id: component2409813
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0055
type: Component
persistent identity: component2409826
display id: component2409826
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component2409833
display id: component2409833
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component2409769
display id: component2409769
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409783
display id: component2409783
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_K1362463
type: Component
persistent identity: component2409842
display id: component2409842
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_G00102
type: Component
persistent identity: component2409781
display id: component2409781
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0043
type: Component
persistent identity: component2409838
display id: component2409838
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409823
display id: annotation2409823
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
type: Range
persistent identity: range2409823
display id: range2409823
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3251
end: 3270
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2409818
display id: annotation2409818
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
component: BBa_P1002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
type: Range
persistent identity: range2409818
display id: range2409818
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2229
end: 3171
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409776
display id: annotation2409776
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
type: Range
persistent identity: range2409776
display id: range2409776
version: 1
direction: direction/reverse
top level: pSB4A50
start: 35
end: 46
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409812
display id: annotation2409812
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
type: Range
persistent identity: range2409812
display id: range2409812
version: 1
direction: direction/reverse
top level: pSB4A50
start: 184
end: 2220
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409779
display id: annotation2409779
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
type: Range
persistent identity: range2409779
display id: range2409779
version: 1
direction: direction/reverse
top level: pSB4A50
start: 47
end: 115
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409826
display id: annotation2409826
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
type: Range
persistent identity: range2409826
display id: range2409826
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3271
end: 3348
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409781
display id: annotation2409781
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
type: Range
persistent identity: range2409781
display id: range2409781
version: 1
direction: direction/reverse
top level: pSB4A50
start: 116
end: 135
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409847
display id: annotation2409847
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
type: Range
persistent identity: range2409847
display id: range2409847
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3425
end: 3446
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409769
display id: annotation2409769
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
type: Range
persistent identity: range2409769
display id: range2409769
version: 1
direction: direction/reverse
top level: pSB4A50
start: 22
end: 34
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409838
display id: annotation2409838
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
type: Range
persistent identity: range2409838
display id: range2409838
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3361
end: 3373
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409821
display id: annotation2409821
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
type: Range
persistent identity: range2409821
display id: range2409821
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3179
end: 3250
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409813
display id: annotation2409813
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
type: Range
persistent identity: range2409813
display id: range2409813
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2221
end: 2228
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409764
display id: annotation2409764
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
type: Range
persistent identity: range2409764
display id: range2409764
version: 1
direction: direction/reverse
top level: pSB4A50
start: 1
end: 21
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409819
display id: annotation2409819
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
type: Range
persistent identity: range2409819
display id: range2409819
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3172
end: 3178
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409842
display id: annotation2409842
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
type: Range
persistent identity: range2409842
display id: range2409842
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3374
end: 3424
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409833
display id: annotation2409833
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
type: Range
persistent identity: range2409833
display id: range2409833
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3349
end: 3360
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409784
display id: annotation2409784
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
type: Range
persistent identity: range2409784
display id: range2409784
version: 1
direction: direction/reverse
top level: pSB4A50
start: 177
end: 183
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409783
display id: annotation2409783
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
type: Range
persistent identity: range2409783
display id: range2409783
version: 1
direction: direction/reverse
top level: pSB4A50
start: 136
end: 176
orientation: inline

BBa_K864012
type: ComponentDefinition
persistent identity: BBa K864012
display id: BBa_K864012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864012_sequence/1

BBa_K1680015
type: ComponentDefinition
persistent identity: BBa K1680015
display id: BBa_K1680015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS315 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:36:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bakteria and URA3 gene for yeast selection.
mutable notes: contains an RFC10 compatible multiple cloning site, no restrictions sites for RFC10 in the Backbone. ADH Terminator after MCS.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: T7 promotor
sequence annotation: RFC10
sequence annotation: ampR promotor
sequence annotation: lacZalpha
sequence annotation: pUC origin
sequence annotation: ampR
sequence annotation: LEU2 promotor
sequence annotation: f1 origin
sequence annotation: lac promotor
sequence annotation: T3 promotor
sequence annotation: CEN/ARS yeast centromere
sequence annotation: LEU2
sequence: https://synbiohub.org/public/igem/BBa_K1680015_sequence/1

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466192
display id: annotation2466192
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466192/range2466192/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466192/range2466192/1
type: Range
persistent identity: range2466192
display id: range2466192
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 3097
end: 3115
orientation: inline

RFC10
type: SequenceAnnotation
persistent identity: annotation2466193
display id: annotation2466193
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466193/range2466193/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466193/range2466193/1
type: Range
persistent identity: range2466193
display id: range2466193
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 3134
end: 3176
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466202
display id: annotation2466202
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466202/range2466202/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466202/range2466202/1
type: Range
persistent identity: range2466202
display id: range2466202
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 5266
end: 5370
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466196
display id: annotation2466196
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466196/range2466196/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466196/range2466196/1
type: Range
persistent identity: range2466196
display id: range2466196
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3186
end: 3248
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466200
display id: annotation2466200
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466200/range2466200/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466200/range2466200/1
type: Range
persistent identity: range2466200
display id: range2466200
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3646
end: 4234
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466201
display id: annotation2466201
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466201/range2466201/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466201/range2466201/1
type: Range
persistent identity: range2466201
display id: range2466201
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 4405
end: 5265
orientation: inline

LEU2 promotor
type: SequenceAnnotation
persistent identity: annotation2466189
display id: annotation2466189
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466189/range2466189/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466189/range2466189/1
type: Range
persistent identity: range2466189
display id: range2466189
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 1770
end: 2174
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2466190
display id: annotation2466190
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466190/range2466190/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466190/range2466190/1
type: Range
persistent identity: range2466190
display id: range2466190
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 2474
end: 2929
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466191
display id: annotation2466191
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466191/range2466191/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466191/range2466191/1
type: Range
persistent identity: range2466191
display id: range2466191
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 2712
end: 3128
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466199
display id: annotation2466199
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466199/range2466199/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466199/range2466199/1
type: Range
persistent identity: range2466199
display id: range2466199
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3292
end: 3322
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466197
display id: annotation2466197
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466197/range2466197/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466197/range2466197/1
type: Range
persistent identity: range2466197
display id: range2466197
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3205
end: 3223
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466204
display id: annotation2466204
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466204/range2466204/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466204/range2466204/1
type: Range
persistent identity: range2466204
display id: range2466204
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 5407
end: 5910
orientation: inline

LEU2
type: SequenceAnnotation
persistent identity: annotation2466188
display id: annotation2466188
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466188/range2466188/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466188/range2466188/1
type: Range
persistent identity: range2466188
display id: range2466188
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 663
end: 1757
orientation: inline

BBa_K864010
type: ComponentDefinition
persistent identity: BBa K864010
display id: BBa_K864010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864010_sequence/1

pSB4C1
type: ComponentDefinition
persistent identity: pSB4C1
display id: pSB4C1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCK01 converted into BioBrick vector (formerly BBa_I739202)
creator: Stefan Luzi
created: 2007-10-23T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Mutagenesis primer
sequence annotation: BioBrick prefix
sequence annotation: chloramphenicol acetyltransferase
sequence annotation: BioBrick suffix
sequence annotation: C->T mutation to eliminate SpeI site
sequence annotation: pSC101 replication initiator protein
sequence: https://synbiohub.org/public/igem/pSB4C1_sequence/1

Mutagenesis primer
type: SequenceAnnotation
persistent identity: annotation1969635
display id: annotation1969635
version: 1
top level: pSB4C1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969635/range1969635/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969635/range1969635/1
type: Range
persistent identity: range1969635
display id: range1969635
version: 1
direction: direction/reverse
top level: pSB4C1
start: 1491
end: 1529
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969636
display id: annotation1969636
version: 1
top level: pSB4C1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969636/range1969636/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969636/range1969636/1
type: Range
persistent identity: range1969636
display id: range1969636
version: 1
direction: direction/forward
top level: pSB4C1
start: 3134
end: 3155
orientation: inline

chloramphenicol acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1969633
display id: annotation1969633
version: 1
top level: pSB4C1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969633/range1969633/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969633/range1969633/1
type: Range
persistent identity: range1969633
display id: range1969633
version: 1
direction: direction/forward
top level: pSB4C1
start: 2311
end: 2970
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969631
display id: annotation1969631
version: 1
top level: pSB4C1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969631/range1969631/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969631/range1969631/1
type: Range
persistent identity: range1969631
display id: range1969631
version: 1
direction: direction/forward
top level: pSB4C1
start: 1
end: 21
orientation: inline

C->T mutation to eliminate SpeI site
type: SequenceAnnotation
persistent identity: annotation1969634
display id: annotation1969634
version: 1
top level: pSB4C1
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969634/range1969634/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969634/range1969634/1
type: Range
persistent identity: range1969634
display id: range1969634
version: 1
direction: direction/forward
top level: pSB4C1
start: 1508
end: 1508
orientation: inline

pSC101 replication initiator protein
type: SequenceAnnotation
persistent identity: annotation1969632
display id: annotation1969632
version: 1
top level: pSB4C1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969632/range1969632/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969632/range1969632/1
type: Range
persistent identity: range1969632
display id: range1969632
version: 1
direction: direction/reverse
top level: pSB4C1
start: 490
end: 1440
orientation: inline

BAC
type: ComponentDefinition
persistent identity: BBa J61031
display id: BBa_J61031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Amplifiable BAC plasmid
creator: John Anderson
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This plasmid is derived originally from pBeloBAC11 with multiple modifications to make it compatible with biobricks and more useable. Without modification, J61031 is a high-copy plasmid in normal laboratory strains of E. coli due to the colE1 origin of replication located within the biobrick part region. It also confers ampicillin resistance, kanamycin resistance, and constitutively-expressed RFP. Upon replacement of the SpeI/XbaI region, the colE1, ampR, and RFP features are removed, leaving behind the F plasmid replicon, the KanR gene, and an R6K origin of replication. At this point, the plasmid will replicate as a medium or high copy plasmid in pir+ or pir116 strains such as Ec100D::pir116. In non-pir strains (most lab strains), the plasmid will replicate at near-single copy levels. The restriction sites EcoRI, SpeI, NotI, and XbaI are all unique in this plasmid. There are several PstI sites, however. pBACr-NESd
mutable notes: N/A
mutable provenance: many many steps
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: GFP reporter
sequence annotation: mRFP
sequence annotation: kanR/neoR promoter
sequence annotation: rrnB T1 terminator
sequence annotation: sopA
sequence annotation: pBR322 origin
sequence annotation: PstI
sequence annotation: c myc tag
sequence annotation: pMB1 replication origin
sequence annotation: R6Kg origin
sequence annotation: 6xHis tag
sequence annotation: repE
sequence annotation: BBa_R0040 Ptet promoter
sequence annotation: kanamycin/neomycin resistance marker (NTP II marker)
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: rrnB terminator
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: SpeI
sequence annotation: finO incD/sopC region; contains 12 43 bp direct repeats that bind SopB protein; required for partitioning of F plasmid to daughter cells after replication as well as repression of sopAB operator;
sequence annotation: NotI
sequence annotation: sopB
sequence annotation: EcoRI
sequence: https://synbiohub.org/public/igem/BBa_J61031_sequence/1

GFP reporter
type: SequenceAnnotation
persistent identity: annotation1976009
display id: annotation1976009
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976009/range1976009/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976009/range1976009/1
type: Range
persistent identity: range1976009
display id: range1976009
version: 1
direction: direction/reverse
top level: BAC
start: 8985
end: 9698
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation1976010
display id: annotation1976010
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976010/range1976010/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976010/range1976010/1
type: Range
persistent identity: range1976010
display id: range1976010
version: 1
direction: direction/forward
top level: BAC
start: 9815
end: 10495
orientation: inline

kanR/neoR promoter
type: SequenceAnnotation
persistent identity: annotation1976001
display id: annotation1976001
version: 1
top level: BAC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976001/range1976001/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976001/range1976001/1
type: Range
persistent identity: range1976001
display id: range1976001
version: 1
direction: direction/forward
top level: BAC
start: 4753
end: 4802
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1976003
display id: annotation1976003
version: 1
top level: BAC
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976003/range1976003/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976003/range1976003/1
type: Range
persistent identity: range1976003
display id: range1976003
version: 1
direction: direction/reverse
top level: BAC
start: 6179
end: 6222
orientation: inline

sopA
type: SequenceAnnotation
persistent identity: annotation1975999
display id: annotation1975999
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1975999/range1975999/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1975999/range1975999/1
type: Range
persistent identity: range1975999
display id: range1975999
version: 1
direction: direction/reverse
top level: BAC
start: 1662
end: 2837
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1934582
display id: annotation1934582
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1934582/range1934582/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1934582/range1934582/1
type: Range
persistent identity: range1934582
display id: range1934582
version: 1
direction: direction/forward
top level: BAC
start: 7864
end: 8303
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976018
display id: annotation1976018
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976018/range1976018/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976018/range1976018/1
type: Range
persistent identity: range1976018
display id: range1976018
version: 1
direction: direction/forward
top level: BAC
start: 8975
end: 8980
orientation: inline

c myc tag
type: SequenceAnnotation
persistent identity: annotation1976006
display id: annotation1976006
version: 1
top level: BAC
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976006/range1976006/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976006/range1976006/1
type: Range
persistent identity: range1976006
display id: range1976006
version: 1
direction: direction/reverse
top level: BAC
start: 6484
end: 6513
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976014
display id: annotation1976014
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976014/range1976014/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976014/range1976014/1
type: Range
persistent identity: range1976014
display id: range1976014
version: 1
direction: direction/forward
top level: BAC
start: 2001
end: 2006
orientation: inline

pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation1934583
display id: annotation1934583
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1934583/range1934583/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1934583/range1934583/1
type: Range
persistent identity: range1934583
display id: range1934583
version: 1
direction: direction/reverse
top level: BAC
start: 7691
end: 8303
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976016
display id: annotation1976016
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976016/range1976016/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976016/range1976016/1
type: Range
persistent identity: range1976016
display id: range1976016
version: 1
direction: direction/forward
top level: BAC
start: 5068
end: 5073
orientation: inline

R6Kg origin
type: SequenceAnnotation
persistent identity: annotation1976002
display id: annotation1976002
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976002/range1976002/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976002/range1976002/1
type: Range
persistent identity: range1976002
display id: range1976002
version: 1
direction: direction/reverse
top level: BAC
start: 5743
end: 6126
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation1976005
display id: annotation1976005
version: 1
top level: BAC
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976005/range1976005/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976005/range1976005/1
type: Range
persistent identity: range1976005
display id: range1976005
version: 1
direction: direction/reverse
top level: BAC
start: 6451
end: 6468
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976015
display id: annotation1976015
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976015/range1976015/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976015/range1976015/1
type: Range
persistent identity: range1976015
display id: range1976015
version: 1
direction: direction/forward
top level: BAC
start: 3542
end: 3547
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1976000
display id: annotation1976000
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976000/range1976000/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976000/range1976000/1
type: Range
persistent identity: range1976000
display id: range1976000
version: 1
direction: direction/reverse
top level: BAC
start: 3416
end: 4171
orientation: inline

BBa_R0040 Ptet promoter
type: SequenceAnnotation
persistent identity: annotation1976021
display id: annotation1976021
version: 1
top level: BAC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976021/range1976021/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976021/range1976021/1
type: Range
persistent identity: range1976021
display id: range1976021
version: 1
direction: direction/forward
top level: BAC
start: 9735
end: 9788
orientation: inline

kanamycin/neomycin resistance marker (NTP II marker)
type: SequenceAnnotation
persistent identity: annotation1934584
display id: annotation1934584
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1934584/range1934584/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1934584/range1934584/1
type: Range
persistent identity: range1934584
display id: range1934584
version: 1
direction: direction/forward
top level: BAC
start: 4891
end: 5685
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1976007
display id: annotation1976007
version: 1
top level: BAC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976007/range1976007/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976007/range1976007/1
type: Range
persistent identity: range1976007
display id: range1976007
version: 1
direction: direction/forward
top level: BAC
start: 6599
end: 6627
orientation: inline

rrnB terminator
type: SequenceAnnotation
persistent identity: annotation1976004
display id: annotation1976004
version: 1
top level: BAC
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976004/range1976004/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976004/range1976004/1
type: Range
persistent identity: range1976004
display id: range1976004
version: 1
direction: direction/reverse
top level: BAC
start: 6188
end: 6345
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1976008
display id: annotation1976008
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976008/range1976008/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976008/range1976008/1
type: Range
persistent identity: range1976008
display id: range1976008
version: 1
direction: direction/forward
top level: BAC
start: 6669
end: 7529
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1976012
display id: annotation1976012
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976012/range1976012/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976012/range1976012/1
type: Range
persistent identity: range1976012
display id: range1976012
version: 1
direction: direction/forward
top level: BAC
start: 2
end: 7
orientation: inline

finO incD/sopC region; contains 12 43 bp direct repeats that bind SopB protein; required for partitioning of F plasmid to daughter cells after replication as well as repression of sopAB operator;
type: SequenceAnnotation
persistent identity: annotation1976020
display id: annotation1976020
version: 1
top level: BAC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976020/range1976020/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976020/range1976020/1
type: Range
persistent identity: range1976020
display id: range1976020
version: 1
direction: direction/reverse
top level: BAC
start: 145
end: 618
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1976013
display id: annotation1976013
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976013/range1976013/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976013/range1976013/1
type: Range
persistent identity: range1976013
display id: range1976013
version: 1
direction: direction/forward
top level: BAC
start: 9
end: 16
orientation: inline

sopB
type: SequenceAnnotation
persistent identity: annotation1975998
display id: annotation1975998
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1975998/range1975998/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1975998/range1975998/1
type: Range
persistent identity: range1975998
display id: range1975998
version: 1
direction: direction/reverse
top level: BAC
start: 691
end: 1662
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1976011
display id: annotation1976011
version: 1
top level: BAC
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976011/range1976011/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976011/range1976011/1
type: Range
persistent identity: range1976011
display id: range1976011
version: 1
direction: direction/forward
top level: BAC
start: 10540
end: 10583
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1976017
display id: annotation1976017
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976017/range1976017/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976017/range1976017/1
type: Range
persistent identity: range1976017
display id: range1976017
version: 1
direction: direction/forward
top level: BAC
start: 6527
end: 6532
orientation: inline

pSB4K50
type: ComponentDefinition
persistent identity: BBa K1362097
display id: BBa_K1362097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick expression backbone carrying Kan resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00000
component: BBa_B0053
component: BBa_B0062
component: BBa_B0042
component: BBa_P1007
component: BBa_B0055
component: BBa_G00102
component: BBa_B0054
component: BBa_I50041
component: BBa_K1362463
component: BBa_B0043
component: BBa_B0044
component: BBa_G0001
component: BBa_G00100
component: BBa_G00001
component: BBa_G0005
component: BBa_G0006
sequence annotation: BBa_G0006
sequence annotation: BBa_B0043
sequence annotation: BBa_K1362463
sequence annotation: BBa_B0062
sequence annotation: BBa_G00102
sequence annotation: BBa_G00100
sequence annotation: BBa_G0001
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_P1007
sequence annotation: BBa_B0042
sequence annotation: BBa_B0055
sequence annotation: BBa_G00000
sequence annotation: BBa_G0005
sequence annotation: BBa_I50041
sequence annotation: BBa_G00001
sequence annotation: BBa_B0054
sequence: https://synbiohub.org/public/igem/BBa_K1362097_sequence/1

BBa_G00000
type: Component
persistent identity: component2410017
display id: component2410017
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0053
type: Component
persistent identity: component2409991
display id: component2409991
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0062
type: Component
persistent identity: component2409956
display id: component2409956
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
type: Component
persistent identity: component2409949
display id: component2409949
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1007
type: Component
persistent identity: component2409961
display id: component2409961
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1007/1

BBa_B0055
type: Component
persistent identity: component2409996
display id: component2409996
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00102
type: Component
persistent identity: component2409954
display id: component2409954
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0054
type: Component
persistent identity: component2409952
display id: component2409952
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_I50041
type: Component
persistent identity: component2409988
display id: component2409988
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50041/1

BBa_K1362463
type: Component
persistent identity: component2410012
display id: component2410012
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_B0043
type: Component
persistent identity: component2410008
display id: component2410008
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component2410003
display id: component2410003
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component2409942
display id: component2409942
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G0001
type: Component
persistent identity: component2409962
display id: component2409962
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0001/1

BBa_G00100
type: Component
persistent identity: component2409993
display id: component2409993
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00001
type: Component
persistent identity: component2409937
display id: component2409937
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G0005
type: Component
persistent identity: component2409957
display id: component2409957
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0005/1

BBa_G0006
type: Component
persistent identity: component2409989
display id: component2409989
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0006/1

BBa_G0006
type: SequenceAnnotation
persistent identity: annotation2409989
display id: annotation2409989
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409989/range2409989/1
component: BBa_G0006

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409989/range2409989/1
type: Range
persistent identity: range2409989
display id: range2409989
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3196
end: 3202
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2410008
display id: annotation2410008
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410008/range2410008/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410008/range2410008/1
type: Range
persistent identity: range2410008
display id: range2410008
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3385
end: 3397
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2410012
display id: annotation2410012
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
type: Range
persistent identity: range2410012
display id: range2410012
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3398
end: 3448
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409956
display id: annotation2409956
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409956/range2409956/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409956/range2409956/1
type: Range
persistent identity: range2409956
display id: range2409956
version: 1
direction: direction/reverse
top level: pSB4K50
start: 136
end: 176
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409954
display id: annotation2409954
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409954/range2409954/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409954/range2409954/1
type: Range
persistent identity: range2409954
display id: range2409954
version: 1
direction: direction/reverse
top level: pSB4K50
start: 116
end: 135
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409993
display id: annotation2409993
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409993/range2409993/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409993/range2409993/1
type: Range
persistent identity: range2409993
display id: range2409993
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3275
end: 3294
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2409962
display id: annotation2409962
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409962/range2409962/1
component: BBa_G0001

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409962/range2409962/1
type: Range
persistent identity: range2409962
display id: range2409962
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1151
end: 1158
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409991
display id: annotation2409991
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409991/range2409991/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409991/range2409991/1
type: Range
persistent identity: range2409991
display id: range2409991
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3203
end: 3274
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409942
display id: annotation2409942
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409942/range2409942/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409942/range2409942/1
type: Range
persistent identity: range2409942
display id: range2409942
version: 1
direction: direction/reverse
top level: pSB4K50
start: 22
end: 34
orientation: inline

BBa_P1007
type: SequenceAnnotation
persistent identity: annotation2409961
display id: annotation2409961
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409961/range2409961/1
component: BBa_P1007

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409961/range2409961/1
type: Range
persistent identity: range2409961
display id: range2409961
version: 1
direction: direction/reverse
top level: pSB4K50
start: 184
end: 1150
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2410003
display id: annotation2410003
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410003/range2410003/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410003/range2410003/1
type: Range
persistent identity: range2410003
display id: range2410003
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3373
end: 3384
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409949
display id: annotation2409949
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409949/range2409949/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409949/range2409949/1
type: Range
persistent identity: range2409949
display id: range2409949
version: 1
direction: direction/reverse
top level: pSB4K50
start: 35
end: 46
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409996
display id: annotation2409996
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409996/range2409996/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409996/range2409996/1
type: Range
persistent identity: range2409996
display id: range2409996
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3295
end: 3372
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2410017
display id: annotation2410017
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410017/range2410017/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410017/range2410017/1
type: Range
persistent identity: range2410017
display id: range2410017
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3449
end: 3470
orientation: inline

BBa_G0005
type: SequenceAnnotation
persistent identity: annotation2409957
display id: annotation2409957
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409957/range2409957/1
component: BBa_G0005

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409957/range2409957/1
type: Range
persistent identity: range2409957
display id: range2409957
version: 1
direction: direction/reverse
top level: pSB4K50
start: 177
end: 183
orientation: inline

BBa_I50041
type: SequenceAnnotation
persistent identity: annotation2409988
display id: annotation2409988
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409988/range2409988/1
component: BBa_I50041

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409988/range2409988/1
type: Range
persistent identity: range2409988
display id: range2409988
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1159
end: 3195
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409937
display id: annotation2409937
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409937/range2409937/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409937/range2409937/1
type: Range
persistent identity: range2409937
display id: range2409937
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1
end: 21
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409952
display id: annotation2409952
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409952/range2409952/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409952/range2409952/1
type: Range
persistent identity: range2409952
display id: range2409952
version: 1
direction: direction/reverse
top level: pSB4K50
start: 47
end: 115
orientation: inline

BBa_K864009
type: ComponentDefinition
persistent identity: BBa K864009
display id: BBa_K864009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rrnC
sequence annotation: Lux terminator
sequence annotation: T3 terminator
sequence annotation: cmR
sequence annotation: -35
sequence annotation: tonB terminator
sequence annotation: pSC101 ori
sequence annotation: lacI
sequence annotation: Promoter
sequence annotation: PlacIq promoter
sequence annotation: RBS
sequence annotation: P22 late terminator
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K864009_sequence/1

rrnC
type: SequenceAnnotation
persistent identity: annotation2202855
display id: annotation2202855
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202855/range2202855/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202855/range2202855/1
type: Range
persistent identity: range2202855
display id: range2202855
version: 1
direction: direction/forward
top level: BBa_K864009
start: 136
end: 175
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2202851
display id: annotation2202851
version: 1
top level: BBa_K864009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202851/range2202851/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202851/range2202851/1
type: Range
persistent identity: range2202851
display id: range2202851
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 3634
end: 3679
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2202853
display id: annotation2202853
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202853/range2202853/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202853/range2202853/1
type: Range
persistent identity: range2202853
display id: range2202853
version: 1
direction: direction/forward
top level: BBa_K864009
start: 4522
end: 4599
orientation: inline

cmR
type: SequenceAnnotation
persistent identity: annotation2202847
display id: annotation2202847
version: 1
top level: BBa_K864009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202847/range2202847/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202847/range2202847/1
type: Range
persistent identity: range2202847
display id: range2202847
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 3686
end: 4348
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2202849
display id: annotation2202849
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202849/range2202849/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202849/range2202849/1
type: Range
persistent identity: range2202849
display id: range2202849
version: 1
direction: direction/forward
top level: BBa_K864009
start: 881
end: 886
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2202854
display id: annotation2202854
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202854/range2202854/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202854/range2202854/1
type: Range
persistent identity: range2202854
display id: range2202854
version: 1
direction: direction/forward
top level: BBa_K864009
start: 47
end: 115
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2202828
display id: annotation2202828
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202828/range2202828/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202828/range2202828/1
type: Range
persistent identity: range2202828
display id: range2202828
version: 1
direction: direction/forward
top level: BBa_K864009
start: 190
end: 2405
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2202846
display id: annotation2202846
version: 1
top level: BBa_K864009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202846/range2202846/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202846/range2202846/1
type: Range
persistent identity: range2202846
display id: range2202846
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2521
end: 3603
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2202844
display id: annotation2202844
version: 1
top level: BBa_K864009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202844/range2202844/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202844/range2202844/1
type: Range
persistent identity: range2202844
display id: range2202844
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 4415
end: 4441
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2202850
display id: annotation2202850
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202850/range2202850/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202850/range2202850/1
type: Range
persistent identity: range2202850
display id: range2202850
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2441
end: 2446
orientation: inline

PlacIq promoter
type: SequenceAnnotation
persistent identity: annotation2202845
display id: annotation2202845
version: 1
top level: BBa_K864009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202845/range2202845/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202845/range2202845/1
type: Range
persistent identity: range2202845
display id: range2202845
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2439
end: 2494
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2202848
display id: annotation2202848
version: 1
top level: BBa_K864009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202848/range2202848/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202848/range2202848/1
type: Range
persistent identity: range2202848
display id: range2202848
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2509
end: 2514
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2202852
display id: annotation2202852
version: 1
top level: BBa_K864009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202852/range2202852/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202852/range2202852/1
type: Range
persistent identity: range2202852
display id: range2202852
version: 1
direction: direction/forward
top level: BBa_K864009
start: 4461
end: 4501
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2202857
display id: annotation2202857
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202857/range2202857/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202857/range2202857/1
type: Range
persistent identity: range2202857
display id: range2202857
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2464
end: 2469
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2202856
display id: annotation2202856
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202856/range2202856/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202856/range2202856/1
type: Range
persistent identity: range2202856
display id: range2202856
version: 1
direction: direction/forward
top level: BBa_K864009
start: 903
end: 908
orientation: inline

BBa_K864002
type: ComponentDefinition
persistent identity: BBa K864002
display id: BBa_K864002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rrnC terminator
sequence annotation: P22 late terminator
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: Lambda Red forward primer
sequence annotation: T3 terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: VF2
sequence annotation: TonB terminator
sequence annotation: Lux terminator
sequence annotation: KanR BBa_P1003
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_K864002_sequence/1

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198834
display id: annotation2198834
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198834/range2198834/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198834/range2198834/1
type: Range
persistent identity: range2198834
display id: range2198834
version: 1
direction: direction/forward
top level: BBa_K864002
start: 136
end: 175
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198839
display id: annotation2198839
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198839/range2198839/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198839/range2198839/1
type: Range
persistent identity: range2198839
display id: range2198839
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3478
end: 3518
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2198835
display id: annotation2198835
version: 1
top level: BBa_K864002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198835/range2198835/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198835/range2198835/1
type: Range
persistent identity: range2198835
display id: range2198835
version: 1
direction: direction/forward
top level: BBa_K864002
start: 190
end: 2405
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198836
display id: annotation2198836
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198836/range2198836/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198836/range2198836/1
type: Range
persistent identity: range2198836
display id: range2198836
version: 1
direction: direction/forward
top level: BBa_K864002
start: 2433
end: 2450
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198841
display id: annotation2198841
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198841/range2198841/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198841/range2198841/1
type: Range
persistent identity: range2198841
display id: range2198841
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3539
end: 3616
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198831
display id: annotation2198831
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198831/range2198831/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198831/range2198831/1
type: Range
persistent identity: range2198831
display id: range2198831
version: 1
direction: direction/reverse
top level: BBa_K864002
start: 18
end: 38
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198840
display id: annotation2198840
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198840/range2198840/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198840/range2198840/1
type: Range
persistent identity: range2198840
display id: range2198840
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3519
end: 3538
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2198832
display id: annotation2198832
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198832/range2198832/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198832/range2198832/1
type: Range
persistent identity: range2198832
display id: range2198832
version: 1
direction: direction/forward
top level: BBa_K864002
start: 47
end: 115
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198837
display id: annotation2198837
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198837/range2198837/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198837/range2198837/1
type: Range
persistent identity: range2198837
display id: range2198837
version: 1
direction: direction/reverse
top level: BBa_K864002
start: 2454
end: 2499
orientation: inline

KanR BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2198838
display id: annotation2198838
version: 1
top level: BBa_K864002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198838/range2198838/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198838/range2198838/1
type: Range
persistent identity: range2198838
display id: range2198838
version: 1
direction: direction/forward
top level: BBa_K864002
start: 2506
end: 3224
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198833
display id: annotation2198833
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198833/range2198833/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198833/range2198833/1
type: Range
persistent identity: range2198833
display id: range2198833
version: 1
direction: direction/forward
top level: BBa_K864002
start: 116
end: 135
orientation: inline

BBa_K864014
type: ComponentDefinition
persistent identity: BBa K864014
display id: BBa_K864014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864014_sequence/1

pSB8A15
type: ComponentDefinition
persistent identity: BBa K864016
display id: BBa_K864016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864016_sequence/1

BBa_K864015
type: ComponentDefinition
persistent identity: BBa K864015
display id: BBa_K864015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4S15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864015_sequence/1

BBa_K1680016
type: ComponentDefinition
persistent identity: BBa K1680016
display id: BBa_K1680016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS316 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:37:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.
mutable notes: Has an RFC10 compatible MCS, with ADH Terminator. No further RFC10 restrictions sites in the backbone.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: CEN/ARS yeast centromere
sequence annotation: URA3 promotor
sequence annotation: ampR
sequence annotation: URA3
sequence annotation: pUC origin
sequence annotation: f1 origin
sequence annotation: T7 promotor
sequence annotation: lacZalpha
sequence annotation: ampR promotor
sequence annotation: T3 promotor
sequence annotation: lac promotor
sequence annotation: RFC10 MCS
sequence: https://synbiohub.org/public/igem/BBa_K1680016_sequence/1

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466237
display id: annotation2466237
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466237/range2466237/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466237/range2466237/1
type: Range
persistent identity: range2466237
display id: range2466237
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 4276
end: 4779
orientation: inline

URA3 promotor
type: SequenceAnnotation
persistent identity: annotation2466206
display id: annotation2466206
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466206/range2466206/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466206/range2466206/1
type: Range
persistent identity: range2466206
display id: range2466206
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 195
end: 410
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466235
display id: annotation2466235
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466235/range2466235/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466235/range2466235/1
type: Range
persistent identity: range2466235
display id: range2466235
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 3274
end: 4134
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2466209
display id: annotation2466209
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466209/range2466209/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466209/range2466209/1
type: Range
persistent identity: range2466209
display id: range2466209
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 411
end: 1214
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466222
display id: annotation2466222
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466222/range2466222/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466222/range2466222/1
type: Range
persistent identity: range2466222
display id: range2466222
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2515
end: 3103
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2466211
display id: annotation2466211
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466211/range2466211/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466211/range2466211/1
type: Range
persistent identity: range2466211
display id: range2466211
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 1343
end: 1798
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466214
display id: annotation2466214
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466214/range2466214/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466214/range2466214/1
type: Range
persistent identity: range2466214
display id: range2466214
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 1966
end: 1985
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466217
display id: annotation2466217
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466217/range2466217/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466217/range2466217/1
type: Range
persistent identity: range2466217
display id: range2466217
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2055
end: 2117
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466236
display id: annotation2466236
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466236/range2466236/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466236/range2466236/1
type: Range
persistent identity: range2466236
display id: range2466236
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 4135
end: 4239
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466219
display id: annotation2466219
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466219/range2466219/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466219/range2466219/1
type: Range
persistent identity: range2466219
display id: range2466219
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2073
end: 2092
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466221
display id: annotation2466221
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466221/range2466221/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466221/range2466221/1
type: Range
persistent identity: range2466221
display id: range2466221
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2161
end: 2191
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466213
display id: annotation2466213
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466213/range2466213/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466213/range2466213/1
type: Range
persistent identity: range2466213
display id: range2466213
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 1581
end: 1997
orientation: inline

RFC10 MCS
type: SequenceAnnotation
persistent identity: annotation2466215
display id: annotation2466215
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466215/range2466215/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466215/range2466215/1
type: Range
persistent identity: range2466215
display id: range2466215
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2003
end: 2045
orientation: inline

pSB4T5
type: ComponentDefinition
persistent identity: pSB4T5
display id: pSB4T5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4T3 is a low copy plasmid with tetracycline resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is constructed from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design for more information.
mutable provenance: BBa_I51001 vector scaffold, BBa_I50040 replication origin and BBa_P1005 resistance marker.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G0004
component: BBa_G00102
component: BBa_B0062
component: BBa_B0044
component: BBa_P1005
component: BBa_G00100
component: BBa_B0055
component: BBa_B0053
component: BBa_G00001
component: BBa_G0003
component: BBa_G0002
component: BBa_B0042
component: BBa_B0043
component: BBa_G00000
component: BBa_B0054
component: BBa_I50042
sequence annotation: BBa_I50042
sequence annotation: BBa_B0042
sequence annotation: BBa_G0003
sequence annotation: BBa_B0062
sequence annotation: BBa_G00000
sequence annotation: BBa_G00100
sequence annotation: BBa_P1005
sequence annotation: BBa_G00102
sequence annotation: BBa_G0002
sequence annotation: BBa_B0044
sequence annotation: BBa_B0055
sequence annotation: BBa_G0004
sequence annotation: BBa_G00001
sequence annotation: BBa_B0053
sequence annotation: BBa_B0043
sequence annotation: BBa_B0054
sequence: https://synbiohub.org/public/igem/pSB4T5_sequence/1

BBa_G0004
type: Component
persistent identity: component2236454
display id: component2236454
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00102
type: Component
persistent identity: component2236418
display id: component2236418
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0062
type: Component
persistent identity: component2236420
display id: component2236420
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0044
type: Component
persistent identity: component2236406
display id: component2236406
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_P1005
type: Component
persistent identity: component2236453
display id: component2236453
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1005/1

BBa_G00100
type: Component
persistent identity: component2236458
display id: component2236458
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0055
type: Component
persistent identity: component2236461
display id: component2236461
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0053
type: Component
persistent identity: component2236456
display id: component2236456
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G00001
type: Component
persistent identity: component2236401
display id: component2236401
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G0003
type: Component
persistent identity: component2236421
display id: component2236421
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G0002
type: Component
persistent identity: component2236450
display id: component2236450
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0042
type: Component
persistent identity: component2236413
display id: component2236413
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component2236473
display id: component2236473
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component2236468
display id: component2236468
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00000
type: Component
persistent identity: component2236478
display id: component2236478
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0054
type: Component
persistent identity: component2236416
display id: component2236416
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_I50042
type: Component
persistent identity: component2236449
display id: component2236449
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236449
display id: annotation2236449
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236449/range2236449/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4T5/annotation2236449/range2236449/1
type: Range
persistent identity: range2236449
display id: range2236449
version: 1
direction: direction/reverse
top level: pSB4T5
start: 184
end: 2220
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236468
display id: annotation2236468
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236468/range2236468/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4T5/annotation2236468/range2236468/1
type: Range
persistent identity: range2236468
display id: range2236468
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3689
end: 3700
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236421
display id: annotation2236421
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236421/range2236421/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4T5/annotation2236421/range2236421/1
type: Range
persistent identity: range2236421
display id: range2236421
version: 1
direction: direction/reverse
top level: pSB4T5
start: 177
end: 183
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236420
display id: annotation2236420
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236420/range2236420/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4T5/annotation2236420/range2236420/1
type: Range
persistent identity: range2236420
display id: range2236420
version: 1
direction: direction/reverse
top level: pSB4T5
start: 136
end: 176
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236478
display id: annotation2236478
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236478/range2236478/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4T5/annotation2236478/range2236478/1
type: Range
persistent identity: range2236478
display id: range2236478
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3714
end: 3735
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236458
display id: annotation2236458
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236458/range2236458/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4T5/annotation2236458/range2236458/1
type: Range
persistent identity: range2236458
display id: range2236458
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3591
end: 3610
orientation: inline

BBa_P1005
type: SequenceAnnotation
persistent identity: annotation2236453
display id: annotation2236453
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236453/range2236453/1
component: BBa_P1005

https://synbiohub.org/public/igem/pSB4T5/annotation2236453/range2236453/1
type: Range
persistent identity: range2236453
display id: range2236453
version: 1
direction: direction/reverse
top level: pSB4T5
start: 2229
end: 3511
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236418
display id: annotation2236418
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236418/range2236418/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4T5/annotation2236418/range2236418/1
type: Range
persistent identity: range2236418
display id: range2236418
version: 1
direction: direction/reverse
top level: pSB4T5
start: 116
end: 135
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236450
display id: annotation2236450
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236450/range2236450/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4T5/annotation2236450/range2236450/1
type: Range
persistent identity: range2236450
display id: range2236450
version: 1
direction: direction/reverse
top level: pSB4T5
start: 2221
end: 2228
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236406
display id: annotation2236406
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236406/range2236406/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4T5/annotation2236406/range2236406/1
type: Range
persistent identity: range2236406
display id: range2236406
version: 1
direction: direction/reverse
top level: pSB4T5
start: 22
end: 34
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236461
display id: annotation2236461
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236461/range2236461/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4T5/annotation2236461/range2236461/1
type: Range
persistent identity: range2236461
display id: range2236461
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3611
end: 3688
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236454
display id: annotation2236454
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236454/range2236454/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4T5/annotation2236454/range2236454/1
type: Range
persistent identity: range2236454
display id: range2236454
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3512
end: 3518
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236413
display id: annotation2236413
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236413/range2236413/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4T5/annotation2236413/range2236413/1
type: Range
persistent identity: range2236413
display id: range2236413
version: 1
direction: direction/reverse
top level: pSB4T5
start: 35
end: 46
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236401
display id: annotation2236401
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236401/range2236401/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4T5/annotation2236401/range2236401/1
type: Range
persistent identity: range2236401
display id: range2236401
version: 1
direction: direction/reverse
top level: pSB4T5
start: 1
end: 21
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236456
display id: annotation2236456
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236456/range2236456/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4T5/annotation2236456/range2236456/1
type: Range
persistent identity: range2236456
display id: range2236456
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3519
end: 3590
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236473
display id: annotation2236473
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236473/range2236473/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4T5/annotation2236473/range2236473/1
type: Range
persistent identity: range2236473
display id: range2236473
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3701
end: 3713
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236416
display id: annotation2236416
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236416/range2236416/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4T5/annotation2236416/range2236416/1
type: Range
persistent identity: range2236416
display id: range2236416
version: 1
direction: direction/reverse
top level: pSB4T5
start: 47
end: 115
orientation: inline

BBa_K864003
type: ComponentDefinition
persistent identity: BBa K864003
display id: BBa_K864003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: P22 late terminator
sequence annotation: Lambda Red forward primer
sequence annotation: VR
sequence annotation: Promoter
sequence annotation: spcR
sequence annotation: Lux terminator
sequence annotation: VF2
sequence annotation: T3 terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: TonB terminator
sequence annotation: rnC terminator
sequence annotation: pSC101 ori BBa_K864050
sequence: https://synbiohub.org/public/igem/BBa_K864003_sequence/1

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2201243
display id: annotation2201243
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201243/range2201243/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201243/range2201243/1
type: Range
persistent identity: range2201243
display id: range2201243
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3608
end: 3648
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2201239
display id: annotation2201239
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201239/range2201239/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201239/range2201239/1
type: Range
persistent identity: range2201239
display id: range2201239
version: 1
direction: direction/forward
top level: BBa_K864003
start: 2433
end: 2450
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2201236
display id: annotation2201236
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201236/range2201236/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201236/range2201236/1
type: Range
persistent identity: range2201236
display id: range2201236
version: 1
direction: direction/forward
top level: BBa_K864003
start: 116
end: 135
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2201242
display id: annotation2201242
version: 1
top level: BBa_K864003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201242/range2201242/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201242/range2201242/1
type: Range
persistent identity: range2201242
display id: range2201242
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 3575
end: 3601
orientation: inline

spcR
type: SequenceAnnotation
persistent identity: annotation2201241
display id: annotation2201241
version: 1
top level: BBa_K864003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201241/range2201241/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201241/range2201241/1
type: Range
persistent identity: range2201241
display id: range2201241
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 2510
end: 3508
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2201240
display id: annotation2201240
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201240/range2201240/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201240/range2201240/1
type: Range
persistent identity: range2201240
display id: range2201240
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 2454
end: 2499
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2201244
display id: annotation2201244
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201244/range2201244/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201244/range2201244/1
type: Range
persistent identity: range2201244
display id: range2201244
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3649
end: 3668
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2201245
display id: annotation2201245
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201245/range2201245/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201245/range2201245/1
type: Range
persistent identity: range2201245
display id: range2201245
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3669
end: 3746
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2201234
display id: annotation2201234
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201234/range2201234/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201234/range2201234/1
type: Range
persistent identity: range2201234
display id: range2201234
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 18
end: 38
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2201235
display id: annotation2201235
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201235/range2201235/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201235/range2201235/1
type: Range
persistent identity: range2201235
display id: range2201235
version: 1
direction: direction/forward
top level: BBa_K864003
start: 47
end: 115
orientation: inline

rnC terminator
type: SequenceAnnotation
persistent identity: annotation2201237
display id: annotation2201237
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201237/range2201237/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201237/range2201237/1
type: Range
persistent identity: range2201237
display id: range2201237
version: 1
direction: direction/forward
top level: BBa_K864003
start: 136
end: 175
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2201238
display id: annotation2201238
version: 1
top level: BBa_K864003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201238/range2201238/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201238/range2201238/1
type: Range
persistent identity: range2201238
display id: range2201238
version: 1
direction: direction/forward
top level: BBa_K864003
start: 190
end: 2405
orientation: inline

pSB8S15FRT
type: ComponentDefinition
persistent identity: BBa K864023
display id: BBa_K864023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8S15(FRT) is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864023_sequence/1

pSB4K5
type: ComponentDefinition
persistent identity: pSB4K5
display id: pSB4K5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4K5 is a low copy plasmid with kanamycin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design for more information.
mutable provenance: BBa_I51001 vector scaffold, BBa_I50040 replication origin and BBa_P1003 antibiotic resistance marker.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00001
component: BBa_B0044
component: BBa_I50041
component: BBa_G00100
component: BBa_B0055
component: BBa_G0001
component: BBa_G0005
component: BBa_G00102
component: BBa_G0006
component: BBa_B0054
component: BBa_B0062
component: BBa_B0042
component: BBa_B0053
component: BBa_B0043
component: BBa_G00000
component: BBa_P1007
sequence annotation: BBa_B0054
sequence annotation: BBa_G0001
sequence annotation: BBa_I50041
sequence annotation: BBa_B0062
sequence annotation: BBa_P1007
sequence annotation: BBa_B0043
sequence annotation: BBa_G0006
sequence annotation: BBa_G00100
sequence annotation: BBa_B0053
sequence annotation: BBa_G00000
sequence annotation: BBa_B0042
sequence annotation: BBa_B0055
sequence annotation: BBa_G00102
sequence annotation: BBa_G0005
sequence annotation: BBa_G00001
sequence annotation: BBa_B0044
sequence: https://synbiohub.org/public/igem/pSB4K5_sequence/1

BBa_G00001
type: Component
persistent identity: component2235979
display id: component2235979
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0044
type: Component
persistent identity: component2235984
display id: component2235984
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_I50041
type: Component
persistent identity: component2236030
display id: component2236030
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50041/1

BBa_G00100
type: Component
persistent identity: component2236035
display id: component2236035
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0055
type: Component
persistent identity: component2236038
display id: component2236038
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G0001
type: Component
persistent identity: component2236004
display id: component2236004
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0001/1

BBa_G0005
type: Component
persistent identity: component2235999
display id: component2235999
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0005/1

BBa_G00102
type: Component
persistent identity: component2235996
display id: component2235996
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0006
type: Component
persistent identity: component2236031
display id: component2236031
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0006/1

BBa_B0054
type: Component
persistent identity: component2235994
display id: component2235994
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0062
type: Component
persistent identity: component2235998
display id: component2235998
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
type: Component
persistent identity: component2235991
display id: component2235991
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0053
type: Component
persistent identity: component2236033
display id: component2236033
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0043
type: Component
persistent identity: component2236050
display id: component2236050
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00000
type: Component
persistent identity: component2236055
display id: component2236055
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0042
type: Component
persistent identity: component2236045
display id: component2236045
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1007
type: Component
persistent identity: component2236003
display id: component2236003
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1007/1

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2235994
display id: annotation2235994
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235994/range2235994/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4K5/annotation2235994/range2235994/1
type: Range
persistent identity: range2235994
display id: range2235994
version: 1
direction: direction/reverse
top level: pSB4K5
start: 47
end: 115
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2236004
display id: annotation2236004
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236004/range2236004/1
component: BBa_G0001

https://synbiohub.org/public/igem/pSB4K5/annotation2236004/range2236004/1
type: Range
persistent identity: range2236004
display id: range2236004
version: 1
direction: direction/reverse
top level: pSB4K5
start: 1151
end: 1158
orientation: inline

BBa_I50041
type: SequenceAnnotation
persistent identity: annotation2236030
display id: annotation2236030
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236030/range2236030/1
component: BBa_I50041

https://synbiohub.org/public/igem/pSB4K5/annotation2236030/range2236030/1
type: Range
persistent identity: range2236030
display id: range2236030
version: 1
direction: direction/reverse
top level: pSB4K5
start: 1159
end: 3195
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2235998
display id: annotation2235998
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235998/range2235998/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4K5/annotation2235998/range2235998/1
type: Range
persistent identity: range2235998
display id: range2235998
version: 1
direction: direction/reverse
top level: pSB4K5
start: 136
end: 176
orientation: inline

BBa_P1007
type: SequenceAnnotation
persistent identity: annotation2236003
display id: annotation2236003
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236003/range2236003/1
component: BBa_P1007

https://synbiohub.org/public/igem/pSB4K5/annotation2236003/range2236003/1
type: Range
persistent identity: range2236003
display id: range2236003
version: 1
direction: direction/reverse
top level: pSB4K5
start: 184
end: 1150
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236050
display id: annotation2236050
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236050/range2236050/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4K5/annotation2236050/range2236050/1
type: Range
persistent identity: range2236050
display id: range2236050
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3385
end: 3397
orientation: inline

BBa_G0006
type: SequenceAnnotation
persistent identity: annotation2236031
display id: annotation2236031
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236031/range2236031/1
component: BBa_G0006

https://synbiohub.org/public/igem/pSB4K5/annotation2236031/range2236031/1
type: Range
persistent identity: range2236031
display id: range2236031
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3196
end: 3202
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236035
display id: annotation2236035
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236035/range2236035/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4K5/annotation2236035/range2236035/1
type: Range
persistent identity: range2236035
display id: range2236035
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3275
end: 3294
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236033
display id: annotation2236033
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236033/range2236033/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4K5/annotation2236033/range2236033/1
type: Range
persistent identity: range2236033
display id: range2236033
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3203
end: 3274
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236055
display id: annotation2236055
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236055/range2236055/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4K5/annotation2236055/range2236055/1
type: Range
persistent identity: range2236055
display id: range2236055
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3398
end: 3419
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236045
display id: annotation2236045
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236045/range2236045/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4K5/annotation2236045/range2236045/1
type: Range
persistent identity: range2236045
display id: range2236045
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3373
end: 3384
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2235991
display id: annotation2235991
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235991/range2235991/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4K5/annotation2235991/range2235991/1
type: Range
persistent identity: range2235991
display id: range2235991
version: 1
direction: direction/reverse
top level: pSB4K5
start: 35
end: 46
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236038
display id: annotation2236038
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236038/range2236038/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4K5/annotation2236038/range2236038/1
type: Range
persistent identity: range2236038
display id: range2236038
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3295
end: 3372
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2235996
display id: annotation2235996
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235996/range2235996/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4K5/annotation2235996/range2235996/1
type: Range
persistent identity: range2235996
display id: range2235996
version: 1
direction: direction/reverse
top level: pSB4K5
start: 116
end: 135
orientation: inline

BBa_G0005
type: SequenceAnnotation
persistent identity: annotation2235999
display id: annotation2235999
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235999/range2235999/1
component: BBa_G0005

https://synbiohub.org/public/igem/pSB4K5/annotation2235999/range2235999/1
type: Range
persistent identity: range2235999
display id: range2235999
version: 1
direction: direction/reverse
top level: pSB4K5
start: 177
end: 183
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2235979
display id: annotation2235979
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235979/range2235979/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4K5/annotation2235979/range2235979/1
type: Range
persistent identity: range2235979
display id: range2235979
version: 1
direction: direction/reverse
top level: pSB4K5
start: 1
end: 21
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2235984
display id: annotation2235984
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235984/range2235984/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4K5/annotation2235984/range2235984/1
type: Range
persistent identity: range2235984
display id: range2235984
version: 1
direction: direction/reverse
top level: pSB4K5
start: 22
end: 34
orientation: inline

BBa_K1680014
type: ComponentDefinition
persistent identity: BBa K1680014
display id: BBa_K1680014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS313 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:47:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.
mutable notes: contains an ADH Terminator and restrictions sites for the RFC10 standard only in the multiple cloning site, no restrictions sites for RCF10 enzymes in the backbone.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RFC10 MCS
sequence annotation: HIS3
sequence annotation: T3 promotor
sequence annotation: ampR promotor
sequence annotation: CEN/ARS yeast centromere
sequence annotation: pUC origin
sequence annotation: T7 promotor
sequence annotation: lac promotor
sequence annotation: lacZalpha
sequence annotation: ampR
sequence annotation: HIS3 promotor
sequence annotation: f1 ori
sequence: https://synbiohub.org/public/igem/BBa_K1680014_sequence/1

RFC10 MCS
type: SequenceAnnotation
persistent identity: annotation2466135
display id: annotation2466135
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466135/range2466135/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466135/range2466135/1
type: Range
persistent identity: range2466135
display id: range2466135
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 2083
end: 2125
orientation: inline

HIS3
type: SequenceAnnotation
persistent identity: annotation2466098
display id: annotation2466098
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466098/range2466098/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466098/range2466098/1
type: Range
persistent identity: range2466098
display id: range2466098
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 503
end: 1162
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466125
display id: annotation2466125
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466125/range2466125/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466125/range2466125/1
type: Range
persistent identity: range2466125
display id: range2466125
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2153
end: 2172
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466130
display id: annotation2466130
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466130/range2466130/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466130/range2466130/1
type: Range
persistent identity: range2466130
display id: range2466130
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 4215
end: 4319
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466131
display id: annotation2466131
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466131/range2466131/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466131/range2466131/1
type: Range
persistent identity: range2466131
display id: range2466131
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 4356
end: 4859
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466128
display id: annotation2466128
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466128/range2466128/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466128/range2466128/1
type: Range
persistent identity: range2466128
display id: range2466128
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2595
end: 3183
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466133
display id: annotation2466133
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466133/range2466133/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466133/range2466133/1
type: Range
persistent identity: range2466133
display id: range2466133
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 2046
end: 2065
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466127
display id: annotation2466127
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466127/range2466127/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466127/range2466127/1
type: Range
persistent identity: range2466127
display id: range2466127
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2241
end: 2271
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466100
display id: annotation2466100
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466100/range2466100/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466100/range2466100/1
type: Range
persistent identity: range2466100
display id: range2466100
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 1661
end: 2077
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466129
display id: annotation2466129
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466129/range2466129/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466129/range2466129/1
type: Range
persistent identity: range2466129
display id: range2466129
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 3354
end: 4214
orientation: inline

HIS3 promotor
type: SequenceAnnotation
persistent identity: annotation2466090
display id: annotation2466090
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466090/range2466090/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466090/range2466090/1
type: Range
persistent identity: range2466090
display id: range2466090
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 315
end: 502
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation2466099
display id: annotation2466099
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466099/range2466099/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466099/range2466099/1
type: Range
persistent identity: range2466099
display id: range2466099
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 1423
end: 1878
orientation: inline

pSB4A3
type: ComponentDefinition
persistent identity: pSB4A3
display id: pSB4A3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A3 (Replaced by pSB4A5)
creator: Reshma Shetty
created: 2004-06-28T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB4A3-1 is a low copy plasmid with ampicillin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) pSB4A3 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into any DNA outside the MCS.The orientation of the pBLA promoter driving the AmpR gene is such that relatively little transcription should be coming into the MCS from upstream. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator stem loop
sequence annotation: Terminator (rev)
sequence annotation: suffix
sequence annotation: RBS (rev)
sequence annotation: repA (rev)
sequence annotation: VF2
sequence annotation: -35
sequence annotation: (rev)
sequence annotation: deletion of two bases
sequence annotation: E.coli His operon terminator
sequence annotation: -10
sequence annotation: rbs(rev)
sequence annotation: prefix
sequence annotation: VR
sequence annotation: AmpR (rev)
sequence annotation: end of terminator
sequence: https://synbiohub.org/public/igem/pSB4A3_sequence/1

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824031
display id: annotation1824031
version: 1
top level: pSB4A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB4A3/annotation1824031/range1824031/1

https://synbiohub.org/public/igem/pSB4A3/annotation1824031/range1824031/1
type: Range
persistent identity: range1824031
display id: range1824031
version: 1
direction: direction/forward
top level: pSB4A3
start: 30
end: 64
orientation: inline

Terminator (rev)
type: SequenceAnnotation
persistent identity: annotation1782538
display id: annotation1782538
version: 1
top level: pSB4A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782538/range1782538/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782538/range1782538/1
type: Range
persistent identity: range1782538
display id: range1782538
version: 1
direction: direction/reverse
top level: pSB4A3
start: 2145
end: 2174
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1782532
display id: annotation1782532
version: 1
top level: pSB4A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782532/range1782532/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782532/range1782532/1
type: Range
persistent identity: range1782532
display id: range1782532
version: 1
direction: direction/forward
top level: pSB4A3
start: 1
end: 21
orientation: inline

RBS (rev)
type: SequenceAnnotation
persistent identity: annotation1782535
display id: annotation1782535
version: 1
top level: pSB4A3
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782535/range1782535/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782535/range1782535/1
type: Range
persistent identity: range1782535
display id: range1782535
version: 1
direction: direction/reverse
top level: pSB4A3
start: 1338
end: 1350
orientation: inline

repA (rev)
type: SequenceAnnotation
persistent identity: annotation1782534
display id: annotation1782534
version: 1
top level: pSB4A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782534/range1782534/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782534/range1782534/1
type: Range
persistent identity: range1782534
display id: range1782534
version: 1
direction: direction/reverse
top level: pSB4A3
start: 384
end: 1334
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824032
display id: annotation1824032
version: 1
top level: pSB4A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB4A3/annotation1824032/range1824032/1

https://synbiohub.org/public/igem/pSB4A3/annotation1824032/range1824032/1
type: Range
persistent identity: range1824032
display id: range1824032
version: 1
direction: direction/reverse
top level: pSB4A3
start: 3289
end: 3308
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782550
display id: annotation1782550
version: 1
top level: pSB4A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782550/range1782550/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782550/range1782550/1
type: Range
persistent identity: range1782550
display id: range1782550
version: 1
direction: direction/forward
top level: pSB4A3
start: 3200
end: 3219
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1782529
display id: annotation1782529
version: 1
top level: pSB4A3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782529/range1782529/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782529/range1782529/1
type: Range
persistent identity: range1782529
display id: range1782529
version: 1
direction: direction/forward
top level: pSB4A3
start: 3124
end: 3129
orientation: inline

(rev)
type: SequenceAnnotation
persistent identity: annotation1782536
display id: annotation1782536
version: 1
top level: pSB4A3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782536/range1782536/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782536/range1782536/1
type: Range
persistent identity: range1782536
display id: range1782536
version: 1
direction: direction/reverse
top level: pSB4A3
start: 1355
end: 1374
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823771
display id: annotation1823771
version: 1
top level: pSB4A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB4A3/annotation1823771/range1823771/1

https://synbiohub.org/public/igem/pSB4A3/annotation1823771/range1823771/1
type: Range
persistent identity: range1823771
display id: range1823771
version: 1
direction: direction/forward
top level: pSB4A3
start: 34
end: 35
orientation: inline

E.coli His operon terminator
type: SequenceAnnotation
persistent identity: annotation1782533
display id: annotation1782533
version: 1
top level: pSB4A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782533/range1782533/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782533/range1782533/1
type: Range
persistent identity: range1782533
display id: range1782533
version: 1
direction: direction/forward
top level: pSB4A3
start: 22
end: 93
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1782528
display id: annotation1782528
version: 1
top level: pSB4A3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782528/range1782528/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782528/range1782528/1
type: Range
persistent identity: range1782528
display id: range1782528
version: 1
direction: direction/forward
top level: pSB4A3
start: 3098
end: 3103
orientation: inline

rbs(rev)
type: SequenceAnnotation
persistent identity: annotation1782527
display id: annotation1782527
version: 1
top level: pSB4A3
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782527/range1782527/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782527/range1782527/1
type: Range
persistent identity: range1782527
display id: range1782527
version: 1
direction: direction/reverse
top level: pSB4A3
start: 3085
end: 3089
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1782531
display id: annotation1782531
version: 1
top level: pSB4A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782531/range1782531/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782531/range1782531/1
type: Range
persistent identity: range1782531
display id: range1782531
version: 1
direction: direction/forward
top level: pSB4A3
start: 3318
end: 3339
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782542
display id: annotation1782542
version: 1
top level: pSB4A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782542/range1782542/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782542/range1782542/1
type: Range
persistent identity: range1782542
display id: range1782542
version: 1
direction: direction/reverse
top level: pSB4A3
start: 157
end: 176
orientation: inline

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation1782537
display id: annotation1782537
version: 1
top level: pSB4A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782537/range1782537/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782537/range1782537/1
type: Range
persistent identity: range1782537
display id: range1782537
version: 1
direction: direction/reverse
top level: pSB4A3
start: 2220
end: 3080
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1782530
display id: annotation1782530
version: 1
top level: pSB4A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782530/range1782530/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782530/range1782530/1
type: Range
persistent identity: range1782530
display id: range1782530
version: 1
direction: direction/reverse
top level: pSB4A3
start: 3276
end: 3279
orientation: inline

pSB8K15
type: ComponentDefinition
persistent identity: BBa K864018
display id: BBa_K864018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8K15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864018_sequence/1

pSB4A5
type: ComponentDefinition
persistent identity: pSB4A5
display id: pSB4A5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-26T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4A5 is a low copy plasmid with ampicillin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
mutable provenance: BBa_I51001, pSC101 origin and a new version of ampR stripped of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G0002
component: BBa_B0042
component: BBa_P1002
component: BBa_B0055
component: BBa_G0004
component: BBa_G00000
component: BBa_B0044
component: BBa_G00102
component: BBa_B0062
component: BBa_G00100
component: BBa_I50042
component: BBa_B0054
component: BBa_B0043
component: BBa_B0053
component: BBa_G0003
component: BBa_G00001
sequence annotation: BBa_B0053
sequence annotation: BBa_B0062
sequence annotation: BBa_G0003
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_G0004
sequence annotation: BBa_G00000
sequence annotation: BBa_B0043
sequence annotation: BBa_G00102
sequence annotation: BBa_G0002
sequence annotation: BBa_I50042
sequence annotation: BBa_B0054
sequence annotation: BBa_B0055
sequence annotation: BBa_P1002
sequence annotation: BBa_G00001
sequence annotation: BBa_G00100
sequence: https://synbiohub.org/public/igem/pSB4A5_sequence/1

BBa_G0002
type: Component
persistent identity: component2236366
display id: component2236366
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0042
type: Component
persistent identity: component2236386
display id: component2236386
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1002
type: Component
persistent identity: component2236371
display id: component2236371
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_B0055
type: Component
persistent identity: component2236379
display id: component2236379
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G0004
type: Component
persistent identity: component2236372
display id: component2236372
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00000
type: Component
persistent identity: component2236396
display id: component2236396
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0044
type: Component
persistent identity: component2236322
display id: component2236322
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00102
type: Component
persistent identity: component2236334
display id: component2236334
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0062
type: Component
persistent identity: component2236336
display id: component2236336
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
type: Component
persistent identity: component2236329
display id: component2236329
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00100
type: Component
persistent identity: component2236376
display id: component2236376
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_I50042
type: Component
persistent identity: component2236365
display id: component2236365
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0054
type: Component
persistent identity: component2236332
display id: component2236332
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0043
type: Component
persistent identity: component2236391
display id: component2236391
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0053
type: Component
persistent identity: component2236374
display id: component2236374
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0003
type: Component
persistent identity: component2236337
display id: component2236337
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00001
type: Component
persistent identity: component2236317
display id: component2236317
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236374
display id: annotation2236374
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236374/range2236374/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4A5/annotation2236374/range2236374/1
type: Range
persistent identity: range2236374
display id: range2236374
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3179
end: 3250
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236336
display id: annotation2236336
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236336/range2236336/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4A5/annotation2236336/range2236336/1
type: Range
persistent identity: range2236336
display id: range2236336
version: 1
direction: direction/reverse
top level: pSB4A5
start: 136
end: 176
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236337
display id: annotation2236337
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236337/range2236337/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4A5/annotation2236337/range2236337/1
type: Range
persistent identity: range2236337
display id: range2236337
version: 1
direction: direction/reverse
top level: pSB4A5
start: 177
end: 183
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236322
display id: annotation2236322
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236322/range2236322/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4A5/annotation2236322/range2236322/1
type: Range
persistent identity: range2236322
display id: range2236322
version: 1
direction: direction/reverse
top level: pSB4A5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236329
display id: annotation2236329
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236329/range2236329/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4A5/annotation2236329/range2236329/1
type: Range
persistent identity: range2236329
display id: range2236329
version: 1
direction: direction/reverse
top level: pSB4A5
start: 35
end: 46
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236372
display id: annotation2236372
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236372/range2236372/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4A5/annotation2236372/range2236372/1
type: Range
persistent identity: range2236372
display id: range2236372
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3172
end: 3178
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236396
display id: annotation2236396
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236396/range2236396/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4A5/annotation2236396/range2236396/1
type: Range
persistent identity: range2236396
display id: range2236396
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3374
end: 3395
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236386
display id: annotation2236386
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236386/range2236386/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4A5/annotation2236386/range2236386/1
type: Range
persistent identity: range2236386
display id: range2236386
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3349
end: 3360
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236391
display id: annotation2236391
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236391/range2236391/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4A5/annotation2236391/range2236391/1
type: Range
persistent identity: range2236391
display id: range2236391
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3361
end: 3373
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236334
display id: annotation2236334
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236334/range2236334/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4A5/annotation2236334/range2236334/1
type: Range
persistent identity: range2236334
display id: range2236334
version: 1
direction: direction/reverse
top level: pSB4A5
start: 116
end: 135
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236366
display id: annotation2236366
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236366/range2236366/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4A5/annotation2236366/range2236366/1
type: Range
persistent identity: range2236366
display id: range2236366
version: 1
direction: direction/reverse
top level: pSB4A5
start: 2221
end: 2228
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236365
display id: annotation2236365
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236365/range2236365/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4A5/annotation2236365/range2236365/1
type: Range
persistent identity: range2236365
display id: range2236365
version: 1
direction: direction/reverse
top level: pSB4A5
start: 184
end: 2220
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236332
display id: annotation2236332
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236332/range2236332/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4A5/annotation2236332/range2236332/1
type: Range
persistent identity: range2236332
display id: range2236332
version: 1
direction: direction/reverse
top level: pSB4A5
start: 47
end: 115
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236379
display id: annotation2236379
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236379/range2236379/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4A5/annotation2236379/range2236379/1
type: Range
persistent identity: range2236379
display id: range2236379
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3271
end: 3348
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2236371
display id: annotation2236371
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236371/range2236371/1
component: BBa_P1002

https://synbiohub.org/public/igem/pSB4A5/annotation2236371/range2236371/1
type: Range
persistent identity: range2236371
display id: range2236371
version: 1
direction: direction/reverse
top level: pSB4A5
start: 2229
end: 3171
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236317
display id: annotation2236317
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236317/range2236317/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4A5/annotation2236317/range2236317/1
type: Range
persistent identity: range2236317
display id: range2236317
version: 1
direction: direction/reverse
top level: pSB4A5
start: 1
end: 21
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236376
display id: annotation2236376
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236376/range2236376/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4A5/annotation2236376/range2236376/1
type: Range
persistent identity: range2236376
display id: range2236376
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3251
end: 3270
orientation: inline

B. S. Vect
type: ComponentDefinition
persistent identity: BBa K606003
display id: BBa_K606003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Multi-host replicative plasmid, for B. Subtilis and E. Coli
creator: Cyrille Pauthenier
created: 2011-07-24T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: true
dominant: false
experience: experience/Issues
group u list: _778_
m user id: 0
owner id: 8998
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20). This plasmid is ideal for importing parts in Subtilis: all the cloning can be done in E. Coli, as well as the amplification. Once the plasmid is extracted, you can incorporate it directly in B. Subtilis using electroporation. This plasmid is Ampicilyn resistant in E. Coli, and is tetracycline resistant in both B. Subtilis and E. Coli. The biobrick BBa_J04450 is inserted between the E X and SP site, has a negative cloning control in E. Coli
mutable notes: The EcoRI site has to be eliminated and the Biobricks prefixes and suffixes were recovered
mutable provenance: The plasmid was obtained from Harald Putzer laboratory at the IBPC [http://www.ibpc.fr/UPR9073/equipe_HaraldJackie/AccueilJAP_HPGB.htm] . This is the fusion of the commercial plasmids pBC16-1 and pTZ19R via the site EcoRI. This site has been eliminated by the 2011 iGEM Paris Team by quick change mutagenesis, and the multicloning site was transformed into the biobrick standards.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: LacZ alpha disabled by the cloning
sequence annotation: DNA minor replication origin OriL
sequence annotation: SYNPTZ19R
sequence annotation: Tetracycline resistance gene
sequence annotation: DNA: Major Replication origin OriU
sequence annotation: Beta lactamase peptide (ampicilin)
sequence annotation: pT7 promoter
sequence annotation: Biobrick EX
sequence annotation: pBC16-1 plasmid (B.S. plasmid)
sequence annotation: Replication origin for E. Coli
sequence annotation: Biobrick SP
sequence: https://synbiohub.org/public/igem/BBa_K606003_sequence/1

LacZ alpha disabled by the cloning
type: SequenceAnnotation
persistent identity: annotation2124966
display id: annotation2124966
version: 1
top level: B. S. Vect
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124966/range2124966/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124966/range2124966/1
type: Range
persistent identity: range2124966
display id: range2124966
version: 1
direction: direction/reverse
top level: B. S. Vect
start: 3076
end: 3244
orientation: inline

DNA minor replication origin OriL
type: SequenceAnnotation
persistent identity: annotation2124964
display id: annotation2124964
version: 1
top level: B. S. Vect
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124964/range2124964/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124964/range2124964/1
type: Range
persistent identity: range2124964
display id: range2124964
version: 1
direction: direction/forward
top level: B. S. Vect
start: 2120
end: 2398
orientation: inline

SYNPTZ19R
type: SequenceAnnotation
persistent identity: annotation2124967
display id: annotation2124967
version: 1
top level: B. S. Vect
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124967/range2124967/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124967/range2124967/1
type: Range
persistent identity: range2124967
display id: range2124967
version: 1
direction: direction/reverse
top level: B. S. Vect
start: 3078
end: 5904
orientation: inline

Tetracycline resistance gene
type: SequenceAnnotation
persistent identity: annotation2124963
display id: annotation2124963
version: 1
top level: B. S. Vect
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124963/range2124963/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124963/range2124963/1
type: Range
persistent identity: range2124963
display id: range2124963
version: 1
direction: direction/forward
top level: B. S. Vect
start: 80
end: 1468
orientation: inline

DNA: Major Replication origin OriU
type: SequenceAnnotation
persistent identity: annotation2124965
display id: annotation2124965
version: 1
top level: B. S. Vect
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124965/range2124965/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124965/range2124965/1
type: Range
persistent identity: range2124965
display id: range2124965
version: 1
direction: direction/forward
top level: B. S. Vect
start: 2813
end: 2838
orientation: inline

Beta lactamase peptide (ampicilin)
type: SequenceAnnotation
persistent identity: annotation2124968
display id: annotation2124968
version: 1
top level: B. S. Vect
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124968/range2124968/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124968/range2124968/1
type: Range
persistent identity: range2124968
display id: range2124968
version: 1
direction: direction/reverse
top level: B. S. Vect
start: 3835
end: 4692
orientation: inline

pT7 promoter
type: SequenceAnnotation
persistent identity: annotation2124970
display id: annotation2124970
version: 1
top level: B. S. Vect
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124970/range2124970/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124970/range2124970/1
type: Range
persistent identity: range2124970
display id: range2124970
version: 1
direction: direction/forward
top level: B. S. Vect
start: 5871
end: 5893
orientation: inline

Biobrick EX
type: SequenceAnnotation
persistent identity: annotation2124961
display id: annotation2124961
version: 1
top level: B. S. Vect
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124961/range2124961/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124961/range2124961/1
type: Range
persistent identity: range2124961
display id: range2124961
version: 1
direction: direction/reverse
top level: B. S. Vect
start: 1
end: 21
orientation: inline

pBC16-1 plasmid (B.S. plasmid)
type: SequenceAnnotation
persistent identity: annotation2124962
display id: annotation2124962
version: 1
top level: B. S. Vect
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K606003/annotation2124962/range2124962/1

https://synbiohub.org/public/igem/BBa_K606003/annotation2124962/range2124962/1
type: Range
persistent identity: range2124962
display id: range2124962
version: 1
direction: direction/forward
top level: B. S. Vect
start: 22
end: 3072
orientation: inline

Replication origin for E. Coli
type: SequenceAnnotation
persistent identity: annotation2124969
display id: annotation2124969
version: 1
top level: B. S. Vect
role: SO:0000110
role: feature/misc
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Biobrick SP
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