//plasmidbackbone/copynumber/medium
type: Collection
persistent identity: plasmidbackbone copynumber medium
display id: plasmidbackbone_copynumber_medium
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1639013
member: K592200
member: pSB3T5
member: pSB3C5
member: K592202
member: pSB6A1
member: BBa_I714946
member: pSB6A0
member: BBa_J64100
member: pSB3K5
member: pSB3K3

pSB3K5
type: ComponentDefinition
persistent identity: pSB3K5
display id: pSB3K5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-05-02T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K5 is a low to medium copy plasmid based with kanamycin resistance. It has a p15A-derived replication origin. pSB3K5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the kanamycin resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0054
component: BBa_B0053
component: BBa_B0044
component: BBa_B0042
component: BBa_B0043
component: BBa_B0055
component: BBa_B0062
component: BBa_I50032
component: BBa_G00000
component: BBa_G0003
component: BBa_G00100
component: BBa_P1003
component: BBa_G00001
component: BBa_G00102
component: BBa_G0002
component: BBa_G0004
sequence annotation: BBa_B0042
sequence annotation: BBa_G00102
sequence annotation: BBa_P1003
sequence annotation: BBa_B0044
sequence annotation: BBa_B0043
sequence annotation: BBa_B0062
sequence annotation: BBa_B0055
sequence annotation: BBa_B0054
sequence annotation: BBa_G00001
sequence annotation: BBa_G0002
sequence annotation: BBa_G00100
sequence annotation: BBa_G0003
sequence annotation: BBa_B0053
sequence annotation: BBa_I50032
sequence annotation: BBa_G00000
sequence annotation: BBa_G0004
sequence: https://synbiohub.org/public/igem/pSB3K5_sequence/1

BBa_B0054
type: Component
persistent identity: component1932093
display id: component1932093
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0053
type: Component
persistent identity: component1932106
display id: component1932106
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0044
type: Component
persistent identity: component1932083
display id: component1932083
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0042
type: Component
persistent identity: component1932090
display id: component1932090
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component1932122
display id: component1932122
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component1932117
display id: component1932117
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0055
type: Component
persistent identity: component1932110
display id: component1932110
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0062
type: Component
persistent identity: component1932096
display id: component1932096
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_I50032
type: Component
persistent identity: component1932100
display id: component1932100
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_G00000
type: Component
persistent identity: component1932124
display id: component1932124
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G0003
type: Component
persistent identity: component1932097
display id: component1932097
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00100
type: Component
persistent identity: component1932107
display id: component1932107
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_P1003
type: Component
persistent identity: component1932103
display id: component1932103
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1003/1

BBa_G00001
type: Component
persistent identity: component1932078
display id: component1932078
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00102
type: Component
persistent identity: component1932094
display id: component1932094
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0002
type: Component
persistent identity: component1932101
display id: component1932101
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0004
type: Component
persistent identity: component1932104
display id: component1932104
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1932117
display id: annotation1932117
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932117/range1932117/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3K5/annotation1932117/range1932117/1
type: Range
persistent identity: range1932117
display id: range1932117
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2890
end: 2901
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1932094
display id: annotation1932094
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932094/range1932094/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3K5/annotation1932094/range1932094/1
type: Range
persistent identity: range1932094
display id: range1932094
version: 1
direction: direction/reverse
top level: pSB3K5
start: 116
end: 135
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation1932103
display id: annotation1932103
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932103/range1932103/1
component: BBa_P1003

https://synbiohub.org/public/igem/pSB3K5/annotation1932103/range1932103/1
type: Range
persistent identity: range1932103
display id: range1932103
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1746
end: 2712
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1932083
display id: annotation1932083
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932083/range1932083/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3K5/annotation1932083/range1932083/1
type: Range
persistent identity: range1932083
display id: range1932083
version: 1
direction: direction/reverse
top level: pSB3K5
start: 22
end: 34
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1932122
display id: annotation1932122
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932122/range1932122/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3K5/annotation1932122/range1932122/1
type: Range
persistent identity: range1932122
display id: range1932122
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2902
end: 2914
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1932096
display id: annotation1932096
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932096/range1932096/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3K5/annotation1932096/range1932096/1
type: Range
persistent identity: range1932096
display id: range1932096
version: 1
direction: direction/reverse
top level: pSB3K5
start: 136
end: 176
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1932110
display id: annotation1932110
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932110/range1932110/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3K5/annotation1932110/range1932110/1
type: Range
persistent identity: range1932110
display id: range1932110
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2812
end: 2889
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1932090
display id: annotation1932090
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932090/range1932090/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3K5/annotation1932090/range1932090/1
type: Range
persistent identity: range1932090
display id: range1932090
version: 1
direction: direction/reverse
top level: pSB3K5
start: 35
end: 46
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1932093
display id: annotation1932093
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932093/range1932093/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3K5/annotation1932093/range1932093/1
type: Range
persistent identity: range1932093
display id: range1932093
version: 1
direction: direction/reverse
top level: pSB3K5
start: 47
end: 115
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1932078
display id: annotation1932078
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932078/range1932078/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3K5/annotation1932078/range1932078/1
type: Range
persistent identity: range1932078
display id: range1932078
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1
end: 21
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1932101
display id: annotation1932101
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932101/range1932101/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3K5/annotation1932101/range1932101/1
type: Range
persistent identity: range1932101
display id: range1932101
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1738
end: 1745
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1932107
display id: annotation1932107
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932107/range1932107/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3K5/annotation1932107/range1932107/1
type: Range
persistent identity: range1932107
display id: range1932107
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2792
end: 2811
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1932097
display id: annotation1932097
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932097/range1932097/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3K5/annotation1932097/range1932097/1
type: Range
persistent identity: range1932097
display id: range1932097
version: 1
direction: direction/reverse
top level: pSB3K5
start: 177
end: 183
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1932106
display id: annotation1932106
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932106/range1932106/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3K5/annotation1932106/range1932106/1
type: Range
persistent identity: range1932106
display id: range1932106
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2720
end: 2791
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1932100
display id: annotation1932100
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932100/range1932100/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3K5/annotation1932100/range1932100/1
type: Range
persistent identity: range1932100
display id: range1932100
version: 1
direction: direction/reverse
top level: pSB3K5
start: 184
end: 1737
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1932124
display id: annotation1932124
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932124/range1932124/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3K5/annotation1932124/range1932124/1
type: Range
persistent identity: range1932124
display id: range1932124
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2915
end: 2936
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1932104
display id: annotation1932104
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932104/range1932104/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3K5/annotation1932104/range1932104/1
type: Range
persistent identity: range1932104
display id: range1932104
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2713
end: 2719
orientation: inline

pSB6A1
type: ComponentDefinition
persistent identity: pSB6A1
display id: pSB6A1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBR322 converted to BioBrick vector (formally BBa_I739201)
creator: Stefan Luzi
created: 2007-10-22T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: sadf
mutable notes: asdf
mutable provenance: sdf
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: gamma-delta insertion sequence
sequence annotation: partial tetracycline resistance CDS (nonfunctional)
sequence annotation: RNAII transcript
sequence annotation: bla(P3) promoter -35 sequence
sequence annotation: rep pMB1
sequence annotation: beta-lactamase mature peptide
sequence annotation: BioBrick suffix
sequence annotation: H-strand Y effector site
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: BioBrick prefix
sequence annotation: RNAI transcript promoter -10 sequence
sequence annotation: Repeat region 1
sequence annotation: from pSC101
sequence annotation: bla gene
sequence annotation: beta-lactamase (bla gene)
sequence annotation: RNAI transcript
sequence annotation: beta-lactamase signal peptide
sequence annotation: L-strand Y effector site
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: rop
sequence annotation: bla(P3) promoter -10 sequence
sequence annotation: RNAI transcript promoter -35 sequence
sequence: https://synbiohub.org/public/igem/pSB6A1_sequence/1

gamma-delta insertion sequence
type: SequenceAnnotation
persistent identity: annotation1969639
display id: annotation1969639
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969639/range1969639/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969639/range1969639/1
type: Range
persistent identity: range1969639
display id: range1969639
version: 1
direction: direction/reverse
top level: pSB6A1
start: 1157
end: 1161
orientation: inline

partial tetracycline resistance CDS (nonfunctional)
type: SequenceAnnotation
persistent identity: annotation1969638
display id: annotation1969638
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969638/range1969638/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969638/range1969638/1
type: Range
persistent identity: range1969638
display id: range1969638
version: 1
direction: direction/forward
top level: pSB6A1
start: 23
end: 918
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1969644
display id: annotation1969644
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969644/range1969644/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969644/range1969644/1
type: Range
persistent identity: range1969644
display id: range1969644
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2176
end: 2728
orientation: inline

bla(P3) promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969656
display id: annotation1969656
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969656/range1969656/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969656/range1969656/1
type: Range
persistent identity: range1969656
display id: range1969656
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3860
end: 3865
orientation: inline

rep pMB1
type: SequenceAnnotation
persistent identity: annotation1969645
display id: annotation1969645
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969645/range1969645/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969645/range1969645/1
type: Range
persistent identity: range1969645
display id: range1969645
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2161
end: 2775
orientation: inline

beta-lactamase mature peptide
type: SequenceAnnotation
persistent identity: annotation1969653
display id: annotation1969653
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969653/range1969653/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969653/range1969653/1
type: Range
persistent identity: range1969653
display id: range1969653
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2938
end: 3726
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969637
display id: annotation1969637
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969637/range1969637/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969637/range1969637/1
type: Range
persistent identity: range1969637
display id: range1969637
version: 1
direction: direction/forward
top level: pSB6A1
start: 1
end: 21
orientation: inline

H-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1969642
display id: annotation1969642
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969642/range1969642/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969642/range1969642/1
type: Range
persistent identity: range1969642
display id: range1969642
version: 1
direction: direction/forward
top level: pSB6A1
start: 1653
end: 1809
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969651
display id: annotation1969651
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969651/range1969651/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969651/range1969651/1
type: Range
persistent identity: range1969651
display id: range1969651
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2759
end: 2764
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969658
display id: annotation1969658
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969658/range1969658/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969658/range1969658/1
type: Range
persistent identity: range1969658
display id: range1969658
version: 1
direction: direction/forward
top level: pSB6A1
start: 4001
end: 4022
orientation: inline

RNAI transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969649
display id: annotation1969649
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969649/range1969649/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969649/range1969649/1
type: Range
persistent identity: range1969649
display id: range1969649
version: 1
direction: direction/forward
top level: pSB6A1
start: 2604
end: 2609
orientation: inline

Repeat region 1
type: SequenceAnnotation
persistent identity: annotation1969657
display id: annotation1969657
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969657/range1969657/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969657/range1969657/1
type: Range
persistent identity: range1969657
display id: range1969657
version: 1
direction: direction/forward
top level: pSB6A1
start: 2790
end: 4003
orientation: inline

from pSC101
type: SequenceAnnotation
persistent identity: annotation1969640
display id: annotation1969640
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969640/range1969640/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969640/range1969640/1
type: Range
persistent identity: range1969640
display id: range1969640
version: 1
direction: direction/forward
top level: pSB6A1
start: 1278
end: 1404
orientation: inline

bla gene
type: SequenceAnnotation
persistent identity: annotation1969654
display id: annotation1969654
version: 1
top level: pSB6A1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969654/range1969654/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969654/range1969654/1
type: Range
persistent identity: range1969654
display id: range1969654
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3803
end: 3807
orientation: inline

beta-lactamase (bla gene)
type: SequenceAnnotation
persistent identity: annotation1969647
display id: annotation1969647
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969647/range1969647/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969647/range1969647/1
type: Range
persistent identity: range1969647
display id: range1969647
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2935
end: 3795
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1969646
display id: annotation1969646
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969646/range1969646/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969646/range1969646/1
type: Range
persistent identity: range1969646
display id: range1969646
version: 1
direction: direction/forward
top level: pSB6A1
start: 2618
end: 2725
orientation: inline

beta-lactamase signal peptide
type: SequenceAnnotation
persistent identity: annotation1969652
display id: annotation1969652
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969652/range1969652/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969652/range1969652/1
type: Range
persistent identity: range1969652
display id: range1969652
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3727
end: 3795
orientation: inline

L-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1969643
display id: annotation1969643
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969643/range1969643/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969643/range1969643/1
type: Range
persistent identity: range1969643
display id: range1969643
version: 1
direction: direction/reverse
top level: pSB6A1
start: 1993
end: 2056
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969650
display id: annotation1969650
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969650/range1969650/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969650/range1969650/1
type: Range
persistent identity: range1969650
display id: range1969650
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2738
end: 2743
orientation: inline

rop
type: SequenceAnnotation
persistent identity: annotation1969641
display id: annotation1969641
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969641/range1969641/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969641/range1969641/1
type: Range
persistent identity: range1969641
display id: range1969641
version: 1
direction: direction/forward
top level: pSB6A1
start: 1557
end: 1748
orientation: inline

bla(P3) promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969655
display id: annotation1969655
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969655/range1969655/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969655/range1969655/1
type: Range
persistent identity: range1969655
display id: range1969655
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3839
end: 3844
orientation: inline

RNAI transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969648
display id: annotation1969648
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969648/range1969648/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969648/range1969648/1
type: Range
persistent identity: range1969648
display id: range1969648
version: 1
direction: direction/forward
top level: pSB6A1
start: 2582
end: 2587
orientation: inline

pSB6A0
type: ComponentDefinition
persistent identity: BBa I751000
display id: BBa_I751000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBR322 (Pst I site mutated)
creator: Kenichiro Iwasaki
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pBR322 has Pst I site in its Amp resistant gene, so you can't introduce Biobrick site(Pst I included) in this plasmid. For that reason, we introduced a silent mutation at the site. The mutation is A546C(DNA sequence) in the Amp resistant gene. Introducing this mutation allowed us to aim at constructing pBR standard plasmid.
mutable notes: A546C(DNA sequence) = A182A(amino acid sequence)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: Amp resistant gene
sequence annotation: Pst I site(mutated)
sequence: https://synbiohub.org/public/igem/BBa_I751000_sequence/1

Amp resistant gene
type: SequenceAnnotation
persistent identity: annotation1957969
display id: annotation1957969
version: 1
top level: pSB6A0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I751000/annotation1957969/range1957969/1

https://synbiohub.org/public/igem/BBa_I751000/annotation1957969/range1957969/1
type: Range
persistent identity: range1957969
display id: range1957969
version: 1
direction: direction/reverse
top level: pSB6A0
start: 3293
end: 4153
orientation: inline

Pst I site(mutated)
type: SequenceAnnotation
persistent identity: annotation1957970
display id: annotation1957970
version: 1
top level: pSB6A0
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I751000/annotation1957970/range1957970/1

https://synbiohub.org/public/igem/BBa_I751000/annotation1957970/range1957970/1
type: Range
persistent identity: range1957970
display id: range1957970
version: 1
direction: direction/reverse
top level: pSB6A0
start: 3608
end: 3608
orientation: inline

BBa_I714946
type: ComponentDefinition
persistent identity: BBa I714946
display id: BBa_I714946
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCC002
creator: Xili Liu
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2250
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: with a Ptet promoter controled by TetR, a MCS, T1 terminator, p15A ori and Cm resistence gene. copy number: 15~20
mutable notes: to measure the efficiency of different SD signal, the plasmid has a MCS downstream of Ptet where can insert reporters along with SD signal. There is a T1 terminator downstream of MCS to terminate transcription starting from Ptet. Ptet is repressed by TetR carried on this plasmid and can be turned on by adding aTc.
mutable provenance: pCRT-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: C0040
sequence annotation: promoter of cat
sequence annotation: R0040
sequence annotation: p15A ori
sequence annotation: B0010
sequence annotation: cat
sequence annotation: MCS
sequence: https://synbiohub.org/public/igem/BBa_I714946_sequence/1

C0040
type: SequenceAnnotation
persistent identity: annotation1955250
display id: annotation1955250
version: 1
top level: BBa_I714946
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955250/range1955250/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955250/range1955250/1
type: Range
persistent identity: range1955250
display id: range1955250
version: 1
direction: direction/forward
top level: BBa_I714946
start: 1279
end: 1909
orientation: inline

promoter of cat
type: SequenceAnnotation
persistent identity: annotation1955245
display id: annotation1955245
version: 1
top level: BBa_I714946
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955245/range1955245/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955245/range1955245/1
type: Range
persistent identity: range1955245
display id: range1955245
version: 1
direction: direction/reverse
top level: BBa_I714946
start: 74
end: 103
orientation: inline

R0040
type: SequenceAnnotation
persistent identity: annotation1955246
display id: annotation1955246
version: 1
top level: BBa_I714946
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955246/range1955246/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955246/range1955246/1
type: Range
persistent identity: range1955246
display id: range1955246
version: 1
direction: direction/forward
top level: BBa_I714946
start: 130
end: 177
orientation: inline

p15A ori
type: SequenceAnnotation
persistent identity: annotation1955249
display id: annotation1955249
version: 1
top level: BBa_I714946
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955249/range1955249/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955249/range1955249/1
type: Range
persistent identity: range1955249
display id: range1955249
version: 1
direction: direction/forward
top level: BBa_I714946
start: 431
end: 1179
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation1955248
display id: annotation1955248
version: 1
top level: BBa_I714946
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955248/range1955248/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955248/range1955248/1
type: Range
persistent identity: range1955248
display id: range1955248
version: 1
direction: direction/forward
top level: BBa_I714946
start: 298
end: 388
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation1955262
display id: annotation1955262
version: 1
top level: BBa_I714946
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955262/range1955262/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955262/range1955262/1
type: Range
persistent identity: range1955262
display id: range1955262
version: 1
direction: direction/forward
top level: BBa_I714946
start: 2277
end: 2936
orientation: inline

MCS
type: SequenceAnnotation
persistent identity: annotation1955247
display id: annotation1955247
version: 1
top level: BBa_I714946
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I714946/annotation1955247/range1955247/1

https://synbiohub.org/public/igem/BBa_I714946/annotation1955247/range1955247/1
type: Range
persistent identity: range1955247
display id: range1955247
version: 1
direction: direction/forward
top level: BBa_I714946
start: 224
end: 286
orientation: inline

pSB3K3
type: ComponentDefinition
persistent identity: pSB3K3
display id: pSB3K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2004-06-28T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K3-1 is a medium copy plasmid with kanamycin resistance. It has a p15A pMR101-derived replication origin, copy number 20-30. (Lutz and Bujard, 1997) pSB3K3-1 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into the vectorl. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: kanR (rev)
sequence annotation: E. coli his operon terminator
sequence annotation: terminator stem loop
sequence annotation: Prefix
sequence annotation: VR
sequence annotation: end of terminator
sequence annotation: p15A ORI (rev)
sequence annotation: Suffix
sequence annotation: VF2
sequence annotation: deletion of two bases
sequence: https://synbiohub.org/public/igem/pSB3K3_sequence/1

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation1023045
display id: annotation1023045
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1
type: Range
persistent identity: range1023045
display id: range1023045
version: 1
direction: direction/reverse
top level: pSB3K3
start: 239
end: 1054
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824021
display id: annotation1824021
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1
type: Range
persistent identity: range1824021
display id: range1824021
version: 1
direction: direction/forward
top level: pSB3K3
start: 22
end: 93
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824023
display id: annotation1824023
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1
type: Range
persistent identity: range1824023
display id: range1824023
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2700
end: 2719
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1023043
display id: annotation1023043
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1
type: Range
persistent identity: range1023043
display id: range1023043
version: 1
direction: direction/forward
top level: pSB3K3
start: 2729
end: 2750
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782476
display id: annotation1782476
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1
type: Range
persistent identity: range1782476
display id: range1782476
version: 1
direction: direction/reverse
top level: pSB3K3
start: 157
end: 176
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824024
display id: annotation1824024
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1
type: Range
persistent identity: range1824024
display id: range1824024
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2687
end: 2690
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824022
display id: annotation1824022
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1
type: Range
persistent identity: range1824022
display id: range1824022
version: 1
direction: direction/forward
top level: pSB3K3
start: 30
end: 64
orientation: inline

p15A ORI (rev)
type: SequenceAnnotation
persistent identity: annotation1023046
display id: annotation1023046
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1
type: Range
persistent identity: range1023046
display id: range1023046
version: 1
direction: direction/reverse
top level: pSB3K3
start: 1386
end: 2210
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1023044
display id: annotation1023044
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1
type: Range
persistent identity: range1023044
display id: range1023044
version: 1
direction: direction/forward
top level: pSB3K3
start: 1
end: 21
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782459
display id: annotation1782459
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1
type: Range
persistent identity: range1782459
display id: range1782459
version: 1
direction: direction/forward
top level: pSB3K3
start: 2611
end: 2630
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823788
display id: annotation1823788
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1

https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1
type: Range
persistent identity: range1823788
display id: range1823788
version: 1
direction: direction/forward
top level: pSB3K3
start: 34
end: 35
orientation: inline

pSB3C5
type: ComponentDefinition
persistent identity: pSB3C5
display id: pSB3C5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3C5 is a low to medium copy plasmid based with chloramphenicol resistance. It has a p15A-derived replication origin. pSB3C5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the chloramphenicol resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00001
component: BBa_B0053
component: BBa_B0042
component: BBa_P1004
component: BBa_B0055
component: BBa_B0054
component: BBa_G0003
component: BBa_G0004
component: BBa_B0044
component: BBa_B0043
component: BBa_B0062
component: BBa_I50032
component: BBa_G00100
component: BBa_G00000
component: BBa_G00102
component: BBa_G0002
sequence annotation: BBa_B0053
sequence annotation: BBa_B0043
sequence annotation: BBa_G0004
sequence annotation: BBa_G0002
sequence annotation: BBa_G00001
sequence annotation: BBa_B0055
sequence annotation: BBa_B0042
sequence annotation: BBa_B0044
sequence annotation: BBa_G00102
sequence annotation: BBa_B0054
sequence annotation: BBa_P1004
sequence annotation: BBa_I50032
sequence annotation: BBa_G0003
sequence annotation: BBa_G00000
sequence annotation: BBa_G00100
sequence annotation: BBa_B0062
sequence: https://synbiohub.org/public/igem/pSB3C5_sequence/1

BBa_G00001
type: Component
persistent identity: component1928310
display id: component1928310
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0053
type: Component
persistent identity: component1928338
display id: component1928338
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0042
type: Component
persistent identity: component1928349
display id: component1928349
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1004
type: Component
persistent identity: component1928335
display id: component1928335
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0055
type: Component
persistent identity: component1928342
display id: component1928342
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0054
type: Component
persistent identity: component1928325
display id: component1928325
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G0003
type: Component
persistent identity: component1928329
display id: component1928329
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G0004
type: Component
persistent identity: component1928336
display id: component1928336
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0042
type: Component
persistent identity: component1928322
display id: component1928322
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component1928315
display id: component1928315
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0043
type: Component
persistent identity: component1928354
display id: component1928354
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0062
type: Component
persistent identity: component1928328
display id: component1928328
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_I50032
type: Component
persistent identity: component1928332
display id: component1928332
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_G00100
type: Component
persistent identity: component1928339
display id: component1928339
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00000
type: Component
persistent identity: component1928356
display id: component1928356
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00102
type: Component
persistent identity: component1928326
display id: component1928326
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0002
type: Component
persistent identity: component1928333
display id: component1928333
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1928338
display id: annotation1928338
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928338/range1928338/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3C5/annotation1928338/range1928338/1
type: Range
persistent identity: range1928338
display id: range1928338
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2522
end: 2593
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1928354
display id: annotation1928354
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928354/range1928354/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3C5/annotation1928354/range1928354/1
type: Range
persistent identity: range1928354
display id: range1928354
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2704
end: 2716
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1928336
display id: annotation1928336
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928336/range1928336/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3C5/annotation1928336/range1928336/1
type: Range
persistent identity: range1928336
display id: range1928336
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2515
end: 2521
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1928333
display id: annotation1928333
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928333/range1928333/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3C5/annotation1928333/range1928333/1
type: Range
persistent identity: range1928333
display id: range1928333
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1738
end: 1745
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1928310
display id: annotation1928310
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928310/range1928310/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3C5/annotation1928310/range1928310/1
type: Range
persistent identity: range1928310
display id: range1928310
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1
end: 21
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1928342
display id: annotation1928342
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928342/range1928342/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3C5/annotation1928342/range1928342/1
type: Range
persistent identity: range1928342
display id: range1928342
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2614
end: 2691
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928349
display id: annotation1928349
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928349/range1928349/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3C5/annotation1928349/range1928349/1
type: Range
persistent identity: range1928349
display id: range1928349
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2692
end: 2703
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1928315
display id: annotation1928315
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928315/range1928315/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3C5/annotation1928315/range1928315/1
type: Range
persistent identity: range1928315
display id: range1928315
version: 1
direction: direction/reverse
top level: pSB3C5
start: 22
end: 34
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1928326
display id: annotation1928326
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928326/range1928326/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3C5/annotation1928326/range1928326/1
type: Range
persistent identity: range1928326
display id: range1928326
version: 1
direction: direction/reverse
top level: pSB3C5
start: 116
end: 135
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1928325
display id: annotation1928325
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928325/range1928325/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3C5/annotation1928325/range1928325/1
type: Range
persistent identity: range1928325
display id: range1928325
version: 1
direction: direction/reverse
top level: pSB3C5
start: 47
end: 115
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation1928335
display id: annotation1928335
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928335/range1928335/1
component: BBa_P1004

https://synbiohub.org/public/igem/pSB3C5/annotation1928335/range1928335/1
type: Range
persistent identity: range1928335
display id: range1928335
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1746
end: 2514
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1928332
display id: annotation1928332
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928332/range1928332/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3C5/annotation1928332/range1928332/1
type: Range
persistent identity: range1928332
display id: range1928332
version: 1
direction: direction/reverse
top level: pSB3C5
start: 184
end: 1737
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1928329
display id: annotation1928329
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928329/range1928329/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3C5/annotation1928329/range1928329/1
type: Range
persistent identity: range1928329
display id: range1928329
version: 1
direction: direction/reverse
top level: pSB3C5
start: 177
end: 183
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1928356
display id: annotation1928356
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928356/range1928356/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3C5/annotation1928356/range1928356/1
type: Range
persistent identity: range1928356
display id: range1928356
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2717
end: 2738
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1928339
display id: annotation1928339
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928339/range1928339/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3C5/annotation1928339/range1928339/1
type: Range
persistent identity: range1928339
display id: range1928339
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2594
end: 2613
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928322
display id: annotation1928322
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928322/range1928322/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3C5/annotation1928322/range1928322/1
type: Range
persistent identity: range1928322
display id: range1928322
version: 1
direction: direction/reverse
top level: pSB3C5
start: 35
end: 46
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1928328
display id: annotation1928328
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928328/range1928328/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3C5/annotation1928328/range1928328/1
type: Range
persistent identity: range1928328
display id: range1928328
version: 1
direction: direction/reverse
top level: pSB3C5
start: 136
end: 176
orientation: inline

pSB3T5
type: ComponentDefinition
persistent identity: pSB3T5
display id: pSB3T5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3T5 is a low to medium copy plasmid based with tetracycline resistance. It has a p15A-derived replication origin. pSB3T5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the tetracycline resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00100
component: BBa_G0002
component: BBa_I50032
component: BBa_G00000
component: BBa_B0055
component: BBa_G00102
component: BBa_B0042
component: BBa_B0062
component: BBa_B0043
component: BBa_G00001
component: BBa_B0044
component: BBa_P1005
component: BBa_B0053
component: BBa_G0003
component: BBa_B0054
component: BBa_G0004
sequence annotation: BBa_B0053
sequence annotation: BBa_G00001
sequence annotation: BBa_B0062
sequence annotation: BBa_B0055
sequence annotation: BBa_B0054
sequence annotation: BBa_B0044
sequence annotation: BBa_G00100
sequence annotation: BBa_G0004
sequence annotation: BBa_B0042
sequence annotation: BBa_I50032
sequence annotation: BBa_G0003
sequence annotation: BBa_P1005
sequence annotation: BBa_G00000
sequence annotation: BBa_G00102
sequence annotation: BBa_G0002
sequence annotation: BBa_B0043
sequence: https://synbiohub.org/public/igem/pSB3T5_sequence/1

BBa_G00100
type: Component
persistent identity: component1928388
display id: component1928388
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G0002
type: Component
persistent identity: component1928381
display id: component1928381
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_I50032
type: Component
persistent identity: component1928380
display id: component1928380
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_G00000
type: Component
persistent identity: component1928405
display id: component1928405
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0055
type: Component
persistent identity: component1928391
display id: component1928391
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00102
type: Component
persistent identity: component1928374
display id: component1928374
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component1928398
display id: component1928398
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0062
type: Component
persistent identity: component1928376
display id: component1928376
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0043
type: Component
persistent identity: component1928403
display id: component1928403
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component1928370
display id: component1928370
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00001
type: Component
persistent identity: component1928358
display id: component1928358
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0044
type: Component
persistent identity: component1928363
display id: component1928363
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_P1005
type: Component
persistent identity: component1928384
display id: component1928384
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1005/1

BBa_B0053
type: Component
persistent identity: component1928387
display id: component1928387
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0003
type: Component
persistent identity: component1928377
display id: component1928377
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0054
type: Component
persistent identity: component1928373
display id: component1928373
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G0004
type: Component
persistent identity: component1928385
display id: component1928385
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1928387
display id: annotation1928387
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928387/range1928387/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3T5/annotation1928387/range1928387/1
type: Range
persistent identity: range1928387
display id: range1928387
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3036
end: 3107
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1928358
display id: annotation1928358
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928358/range1928358/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3T5/annotation1928358/range1928358/1
type: Range
persistent identity: range1928358
display id: range1928358
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1
end: 21
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1928376
display id: annotation1928376
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928376/range1928376/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3T5/annotation1928376/range1928376/1
type: Range
persistent identity: range1928376
display id: range1928376
version: 1
direction: direction/reverse
top level: pSB3T5
start: 136
end: 176
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1928391
display id: annotation1928391
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928391/range1928391/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3T5/annotation1928391/range1928391/1
type: Range
persistent identity: range1928391
display id: range1928391
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3128
end: 3205
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1928373
display id: annotation1928373
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928373/range1928373/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3T5/annotation1928373/range1928373/1
type: Range
persistent identity: range1928373
display id: range1928373
version: 1
direction: direction/reverse
top level: pSB3T5
start: 47
end: 115
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1928363
display id: annotation1928363
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928363/range1928363/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3T5/annotation1928363/range1928363/1
type: Range
persistent identity: range1928363
display id: range1928363
version: 1
direction: direction/reverse
top level: pSB3T5
start: 22
end: 34
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1928388
display id: annotation1928388
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928388/range1928388/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3T5/annotation1928388/range1928388/1
type: Range
persistent identity: range1928388
display id: range1928388
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3108
end: 3127
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1928385
display id: annotation1928385
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928385/range1928385/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3T5/annotation1928385/range1928385/1
type: Range
persistent identity: range1928385
display id: range1928385
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3029
end: 3035
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928370
display id: annotation1928370
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928370/range1928370/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3T5/annotation1928370/range1928370/1
type: Range
persistent identity: range1928370
display id: range1928370
version: 1
direction: direction/reverse
top level: pSB3T5
start: 35
end: 46
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928398
display id: annotation1928398
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928398/range1928398/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3T5/annotation1928398/range1928398/1
type: Range
persistent identity: range1928398
display id: range1928398
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3206
end: 3217
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1928380
display id: annotation1928380
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928380/range1928380/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3T5/annotation1928380/range1928380/1
type: Range
persistent identity: range1928380
display id: range1928380
version: 1
direction: direction/reverse
top level: pSB3T5
start: 184
end: 1737
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1928377
display id: annotation1928377
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928377/range1928377/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3T5/annotation1928377/range1928377/1
type: Range
persistent identity: range1928377
display id: range1928377
version: 1
direction: direction/reverse
top level: pSB3T5
start: 177
end: 183
orientation: inline

BBa_P1005
type: SequenceAnnotation
persistent identity: annotation1928384
display id: annotation1928384
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928384/range1928384/1
component: BBa_P1005

https://synbiohub.org/public/igem/pSB3T5/annotation1928384/range1928384/1
type: Range
persistent identity: range1928384
display id: range1928384
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1746
end: 3028
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1928405
display id: annotation1928405
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928405/range1928405/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3T5/annotation1928405/range1928405/1
type: Range
persistent identity: range1928405
display id: range1928405
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3231
end: 3252
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1928374
display id: annotation1928374
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928374/range1928374/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3T5/annotation1928374/range1928374/1
type: Range
persistent identity: range1928374
display id: range1928374
version: 1
direction: direction/reverse
top level: pSB3T5
start: 116
end: 135
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1928381
display id: annotation1928381
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928381/range1928381/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3T5/annotation1928381/range1928381/1
type: Range
persistent identity: range1928381
display id: range1928381
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1738
end: 1745
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1928403
display id: annotation1928403
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928403/range1928403/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3T5/annotation1928403/range1928403/1
type: Range
persistent identity: range1928403
display id: range1928403
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3218
end: 3230
orientation: inline

BBa_K1639013
type: ComponentDefinition
persistent identity: BBa K1639013
display id: BBa_K1639013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pColA-KanR-dTer
creator: Mustafa Yılmaz
created: 2015-09-14T11:00:00Z
modified: 2015-09-21T06:36:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 24789
owner id: 20835
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: A
mutable notes: C
mutable provenance: B
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Amp promoter
sequence annotation: suffix
sequence annotation: KanR
sequence annotation: double terminator (B0010-B0012)
sequence annotation: ColA ori
sequence annotation: prefix
sequence: https://synbiohub.org/public/igem/BBa_K1639013_sequence/1

Amp promoter
type: SequenceAnnotation
persistent identity: annotation2476435
display id: annotation2476435
version: 1
top level: BBa_K1639013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476435/range2476435/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476435/range2476435/1
type: Range
persistent identity: range2476435
display id: range2476435
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 770
end: 861
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2476438
display id: annotation2476438
version: 1
top level: BBa_K1639013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476438/range2476438/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476438/range2476438/1
type: Range
persistent identity: range2476438
display id: range2476438
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 1925
end: 1944
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2476436
display id: annotation2476436
version: 1
top level: BBa_K1639013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476436/range2476436/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476436/range2476436/1
type: Range
persistent identity: range2476436
display id: range2476436
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 862
end: 1677
orientation: inline

double terminator (B0010-B0012)
type: SequenceAnnotation
persistent identity: annotation2476437
display id: annotation2476437
version: 1
top level: BBa_K1639013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476437/range2476437/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476437/range2476437/1
type: Range
persistent identity: range2476437
display id: range2476437
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 1711
end: 1839
orientation: inline

ColA ori
type: SequenceAnnotation
persistent identity: annotation2476434
display id: annotation2476434
version: 1
top level: BBa_K1639013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476434/range2476434/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476434/range2476434/1
type: Range
persistent identity: range2476434
display id: range2476434
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 117
end: 752
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2476433
display id: annotation2476433
version: 1
top level: BBa_K1639013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1639013/annotation2476433/range2476433/1

https://synbiohub.org/public/igem/BBa_K1639013/annotation2476433/range2476433/1
type: Range
persistent identity: range2476433
display id: range2476433
version: 1
direction: direction/forward
top level: BBa_K1639013
start: 1
end: 21
orientation: inline

K592200
type: ComponentDefinition
persistent identity: BBa K592200
display id: BBa_K592200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Erik Gullberg, Antonio Ascue Avalos, Erik Lundin
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 10133
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K592200 is a BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: To make the plasmid usable for Lambda Red recombineering, all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable provenance: Salmonella phage P22 late terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Terminator BBa_B0054
sequence annotation: Terminator BBa_B0062
sequence annotation: VF2 site BBa_G00100
sequence annotation: Spectinomycin resistance marker
sequence annotation: Translational stop BBa_B0042
sequence annotation: VR site BBa_G00102
sequence annotation: T3 terminator BBa_B0055
sequence annotation: p15A low to medium copy replication origin BBa_I50032
sequence annotation: Lambda Red R primer
sequence annotation: P22 late terminator
sequence annotation: SpcR promoter
sequence annotation: Lambda red F primer
sequence: https://synbiohub.org/public/igem/BBa_K592200_sequence/1

Terminator BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2136370
display id: annotation2136370
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136370/range2136370/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136370/range2136370/1
type: Range
persistent identity: range2136370
display id: range2136370
version: 1
direction: direction/forward
top level: K592200
start: 47
end: 115
orientation: inline

Terminator BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2136372
display id: annotation2136372
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136372/range2136372/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136372/range2136372/1
type: Range
persistent identity: range2136372
display id: range2136372
version: 1
direction: direction/forward
top level: K592200
start: 136
end: 176
orientation: inline

VF2 site BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2136378
display id: annotation2136378
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136378/range2136378/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136378/range2136378/1
type: Range
persistent identity: range2136378
display id: range2136378
version: 1
direction: direction/forward
top level: K592200
start: 2993
end: 3012
orientation: inline

Spectinomycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2136375
display id: annotation2136375
version: 1
top level: K592200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136375/range2136375/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136375/range2136375/1
type: Range
persistent identity: range2136375
display id: range2136375
version: 1
direction: direction/reverse
top level: K592200
start: 1841
end: 2840
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2136380
display id: annotation2136380
version: 1
top level: K592200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136380/range2136380/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136380/range2136380/1
type: Range
persistent identity: range2136380
display id: range2136380
version: 1
direction: direction/forward
top level: K592200
start: 3091
end: 3102
orientation: inline

VR site BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2136371
display id: annotation2136371
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136371/range2136371/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136371/range2136371/1
type: Range
persistent identity: range2136371
display id: range2136371
version: 1
direction: direction/reverse
top level: K592200
start: 116
end: 135
orientation: inline

T3 terminator BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2136379
display id: annotation2136379
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136379/range2136379/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136379/range2136379/1
type: Range
persistent identity: range2136379
display id: range2136379
version: 1
direction: direction/forward
top level: K592200
start: 3013
end: 3090
orientation: inline

p15A low to medium copy replication origin BBa_I50032
type: SequenceAnnotation
persistent identity: annotation2136373
display id: annotation2136373
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136373/range2136373/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136373/range2136373/1
type: Range
persistent identity: range2136373
display id: range2136373
version: 1
direction: direction/forward
top level: K592200
start: 184
end: 1748
orientation: inline

Lambda Red R primer
type: SequenceAnnotation
persistent identity: annotation2136398
display id: annotation2136398
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136398/range2136398/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136398/range2136398/1
type: Range
persistent identity: range2136398
display id: range2136398
version: 1
direction: direction/reverse
top level: K592200
start: 20
end: 41
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2136369
display id: annotation2136369
version: 1
top level: K592200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136369/range2136369/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136369/range2136369/1
type: Range
persistent identity: range2136369
display id: range2136369
version: 1
direction: direction/forward
top level: K592200
start: 35
end: 46
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2136377
display id: annotation2136377
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136377/range2136377/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136377/range2136377/1
type: Range
persistent identity: range2136377
display id: range2136377
version: 1
direction: direction/forward
top level: K592200
start: 2952
end: 2992
orientation: inline

SpcR promoter
type: SequenceAnnotation
persistent identity: annotation2136376
display id: annotation2136376
version: 1
top level: K592200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136376/range2136376/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136376/range2136376/1
type: Range
persistent identity: range2136376
display id: range2136376
version: 1
direction: direction/reverse
top level: K592200
start: 2907
end: 2933
orientation: inline

Lambda red F primer
type: SequenceAnnotation
persistent identity: annotation2136374
display id: annotation2136374
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136374/range2136374/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136374/range2136374/1
type: Range
persistent identity: range2136374
display id: range2136374
version: 1
direction: direction/forward
top level: K592200
start: 1726
end: 1745
orientation: inline

BBa_J64100
type: ComponentDefinition
persistent identity: BBa J64100
display id: BBa_J64100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium copy ColE1 BioBrick vector
creator: Jeffrey J Tabor
created: 2007-04-02T11:00:00Z
modified: 2015-06-15T12:42:46Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _98_
m user id: 4206
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable description: This plasmid is based on pProTetE333 (clontech). A SpeI site was removed via an A-T mutation at position 1166 on the original plasmid, resulting in pProTetE333b. The G00100-G00101 fragment of pSB4A3-1 which includes P1010 between a biobricks multiple cloning site which itself is flanked by + strand transcriptional terminators was then cloned between the XhoI and AvrII sites of pProTetE333b. The resulting plasmid carries a ColE1 origin of replication, a Chloramphenicol resistance marker, the biobricks MCS and the ccdAB death cassette.
mutable notes: None
mutable provenance: pProTetE333 (clontech) and biobricks parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: suffix
sequence annotation: prefix
sequence annotation: G00101
sequence annotation: chloramphenicol resistance marker
sequence annotation: P1010
sequence annotation: G00100
sequence annotation: ColE1 origin
sequence: https://synbiohub.org/public/igem/BBa_J64100_sequence/1

suffix
type: SequenceAnnotation
persistent identity: annotation1923843
display id: annotation1923843
version: 1
top level: BBa_J64100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923843/range1923843/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923843/range1923843/1
type: Range
persistent identity: range1923843
display id: range1923843
version: 1
direction: direction/forward
top level: BBa_J64100
start: 821
end: 841
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1923841
display id: annotation1923841
version: 1
top level: BBa_J64100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923841/range1923841/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923841/range1923841/1
type: Range
persistent identity: range1923841
display id: range1923841
version: 1
direction: direction/forward
top level: BBa_J64100
start: 124
end: 145
orientation: inline

G00101
type: SequenceAnnotation
persistent identity: annotation1923844
display id: annotation1923844
version: 1
top level: BBa_J64100
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923844/range1923844/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923844/range1923844/1
type: Range
persistent identity: range1923844
display id: range1923844
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 977
end: 996
orientation: inline

chloramphenicol resistance marker
type: SequenceAnnotation
persistent identity: annotation1923846
display id: annotation1923846
version: 1
top level: BBa_J64100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923846/range1923846/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923846/range1923846/1
type: Range
persistent identity: range1923846
display id: range1923846
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 1939
end: 2598
orientation: inline

G00101
type: SequenceAnnotation
persistent identity: annotation1931328
display id: annotation1931328
version: 1
top level: BBa_J64100
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1931328/range1931328/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1931328/range1931328/1
type: Range
persistent identity: range1931328
display id: range1931328
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 1029
end: 1048
orientation: inline

P1010
type: SequenceAnnotation
persistent identity: annotation1923842
display id: annotation1923842
version: 1
top level: BBa_J64100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923842/range1923842/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923842/range1923842/1
type: Range
persistent identity: range1923842
display id: range1923842
version: 1
direction: direction/forward
top level: BBa_J64100
start: 146
end: 820
orientation: inline

G00100
type: SequenceAnnotation
persistent identity: annotation1923840
display id: annotation1923840
version: 1
top level: BBa_J64100
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923840/range1923840/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923840/range1923840/1
type: Range
persistent identity: range1923840
display id: range1923840
version: 1
direction: direction/forward
top level: BBa_J64100
start: 6
end: 25
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1923845
display id: annotation1923845
version: 1
top level: BBa_J64100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923845/range1923845/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923845/range1923845/1
type: Range
persistent identity: range1923845
display id: range1923845
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 1130
end: 1812
orientation: inline

K592202
type: ComponentDefinition
persistent identity: BBa K592202
display id: BBa_K592202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy Lambda Red recombineering compatible plasmid
creator: Antonio Ascue Avalos
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 10133
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K592202 is a BioBrick friendly vector with low to medium copy p15A replication origin (BBa_I50032) and a kanamycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22, and the kanamycin resistance marker is flanked by FRT sites so that the resistance marker can be removed after chromosomal integration using the FLP recombinase.
mutable notes: To make the plasmid usable for Lambda Red recombineering, all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. To be able to remove the antibiotic resistance marker after chromosomal integration, the kanamycin marker from the pKD4 plasmid (Datsenko and Wanner, 2000) was used. The resistance marker can be removed after chromosomal integration using the FLP recombinase, leaving only the plasmid insert and a scar behind.
mutable provenance: The P22 late terminator come from the genome of the Salmonella phage P22 (GenBank: AF217253.1, 19662..19702), and the kanamycin resistance marker flanked by FRT sites comes from the pKD4 plasmid (Datsenko and Wanner, 2000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Translational stop BBa_B0042
sequence annotation: Terminator BBa_B0054
sequence annotation: VR site BBa_G00102
sequence annotation: VF2 site BBa_G00100
sequence annotation: Kanamycin resistance marker
sequence annotation: Terminator BBa_B0062
sequence annotation: P22 late terminator
sequence annotation: L-red_F_primer
sequence annotation: FRT site
sequence annotation: T3 terminator BBa_B0055
sequence annotation: p15A ori BBa_I50032
sequence annotation: L-red_R_primer
sequence: https://synbiohub.org/public/igem/BBa_K592202_sequence/1

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2137804
display id: annotation2137804
version: 1
top level: K592202
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137804/range2137804/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137804/range2137804/1
type: Range
persistent identity: range2137804
display id: range2137804
version: 1
direction: direction/forward
top level: K592202
start: 35
end: 46
orientation: inline

Terminator BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2137820
display id: annotation2137820
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137820/range2137820/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137820/range2137820/1
type: Range
persistent identity: range2137820
display id: range2137820
version: 1
direction: direction/forward
top level: K592202
start: 47
end: 115
orientation: inline

VR site BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2137821
display id: annotation2137821
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137821/range2137821/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137821/range2137821/1
type: Range
persistent identity: range2137821
display id: range2137821
version: 1
direction: direction/reverse
top level: K592202
start: 116
end: 135
orientation: inline

VF2 site BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2137829
display id: annotation2137829
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137829/range2137829/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137829/range2137829/1
type: Range
persistent identity: range2137829
display id: range2137829
version: 1
direction: direction/forward
top level: K592202
start: 3286
end: 3305
orientation: inline

Kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2137826
display id: annotation2137826
version: 1
top level: K592202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137826/range2137826/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137826/range2137826/1
type: Range
persistent identity: range2137826
display id: range2137826
version: 1
direction: direction/reverse
top level: K592202
start: 2017
end: 2811
orientation: inline

Terminator BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2137822
display id: annotation2137822
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137822/range2137822/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137822/range2137822/1
type: Range
persistent identity: range2137822
display id: range2137822
version: 1
direction: direction/forward
top level: K592202
start: 136
end: 176
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2137828
display id: annotation2137828
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137828/range2137828/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137828/range2137828/1
type: Range
persistent identity: range2137828
display id: range2137828
version: 1
direction: direction/forward
top level: K592202
start: 3245
end: 3285
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2137846
display id: annotation2137846
version: 1
top level: K592202
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137846/range2137846/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137846/range2137846/1
type: Range
persistent identity: range2137846
display id: range2137846
version: 1
direction: direction/forward
top level: K592202
start: 3384
end: 3395
orientation: inline

L-red_F_primer
type: SequenceAnnotation
persistent identity: annotation2137824
display id: annotation2137824
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137824/range2137824/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137824/range2137824/1
type: Range
persistent identity: range2137824
display id: range2137824
version: 1
direction: direction/forward
top level: K592202
start: 1726
end: 1745
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2137827
display id: annotation2137827
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137827/range2137827/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137827/range2137827/1
type: Range
persistent identity: range2137827
display id: range2137827
version: 1
direction: direction/forward
top level: K592202
start: 3172
end: 3219
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2137825
display id: annotation2137825
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137825/range2137825/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137825/range2137825/1
type: Range
persistent identity: range2137825
display id: range2137825
version: 1
direction: direction/forward
top level: K592202
start: 1781
end: 1826
orientation: inline

T3 terminator BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2137830
display id: annotation2137830
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137830/range2137830/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137830/range2137830/1
type: Range
persistent identity: range2137830
display id: range2137830
version: 1
direction: direction/forward
top level: K592202
start: 3306
end: 3383
orientation: inline

p15A ori BBa_I50032
type: SequenceAnnotation
persistent identity: annotation2137823
display id: annotation2137823
version: 1
top level: K592202
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137823/range2137823/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137823/range2137823/1
type: Range
persistent identity: range2137823
display id: range2137823
version: 1
direction: direction/forward
top level: K592202
start: 184
end: 1748
orientation: inline

L-red_R_primer
type: SequenceAnnotation
persistent identity: annotation2137792
display id: annotation2137792
version: 1
top level: K592202
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592202/annotation2137792/range2137792/1

https://synbiohub.org/public/igem/BBa_K592202/annotation2137792/range2137792/1
type: Range
persistent identity: range2137792
display id: range2137792
version: 1
direction: direction/reverse
top level: K592202
start: 20
end: 41
orientation: inline