//plasmidbackbone/expression/inducible
type: Collection
persistent identity: plasmidbackbone expression inducible
display id: plasmidbackbone_expression_inducible
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: xyl+efe
member: IPTG induc
member: BBa_K081006
member: pSB4A50
member: pSBBs4S
member: BBa_S03511
member: pSB1A30
member: BBa_K215000
member: BBa_K1680026
member: pSBBs0K
member: BBa_J04500
member: spac+efe
member: pSB4K50
member: BBa_K345667
member: BBa_J13023
member: pSB4C50
member: BBa_K801004

IPTG induc
type: ComponentDefinition
persistent identity: BBa K653003
display id: BBa_K653003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Expression Platform
creator: Ernesto G??mez
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:13:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _830_
m user id: 0
owner id: 7119
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: We take advances in genetic engineering as synthetic biology, as viable solution to oil pollution cleanup. But for do this; we need to design and build an expression platform that works into our modified E.coli. For this, We choose four standardized biological parts from the Registry, those parts are: IPTG inducible promoter, Ribosomal Binding Site (RBS), GFP Reporter and Double Terminator; Were selected using rationale from molecular biology and genetic engineering to enable us to build up rationally-engineered, our expression platform. For the iGEM 2011, we will integrate and assembly those parts (P+RBS+GFP+T) along with our BioBrick part using the 3A Assembly Method, to bring up an expression device (P+RBS+rhlAB_BB+GFP+T) for rhamnolipid production in E. coli.
mutable notes: Those parts were assembled following the 3A assembly method.
mutable provenance: IPTG inducible promoter with RBS http://partsregistry.org/wiki/index.php?title=Part:BBa_J04500 GFP reporter plus Double Terminator http://partsregistry.org/wiki/index.php/Part:BBa_K653001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J04500
component: BBa_K653001
sequence annotation: BBa_J04500
sequence annotation: BBa_K653001
sequence: https://synbiohub.org/public/igem/BBa_K653003_sequence/1

BBa_J04500
type: Component
persistent identity: component2146654
display id: component2146654
version: 1
top level: IPTG induc
access: public
definition: BBa_J04500

BBa_K653001
type: Component
persistent identity: component2146664
display id: component2146664
version: 1
top level: IPTG induc
access: public
definition: https://synbiohub.org/public/igem/BBa_K653001/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2146654
display id: annotation2146654
version: 1
top level: IPTG induc
location: https://synbiohub.org/public/igem/BBa_K653003/annotation2146654/range2146654/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K653003/annotation2146654/range2146654/1
type: Range
persistent identity: range2146654
display id: range2146654
version: 1
direction: direction/reverse
top level: IPTG induc
start: 1
end: 220
orientation: inline

BBa_K653001
type: SequenceAnnotation
persistent identity: annotation2146664
display id: annotation2146664
version: 1
top level: IPTG induc
location: https://synbiohub.org/public/igem/BBa_K653003/annotation2146664/range2146664/1
component: BBa_K653001

https://synbiohub.org/public/igem/BBa_K653003/annotation2146664/range2146664/1
type: Range
persistent identity: range2146664
display id: range2146664
version: 1
direction: direction/reverse
top level: IPTG induc
start: 227
end: 1083
orientation: inline

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible promoter with RBS
creator: Kristen DeCelle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0010.B0034
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
direction: direction/forward
top level: BBa_J04500
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
direction: direction/forward
top level: BBa_J04500
start: 1
end: 200
orientation: inline

BBa_K345667
type: ComponentDefinition
persistent identity: BBa K345667
display id: BBa_K345667
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac regulated insertable promoter
creator: Florencia Camus
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _547_
m user id: 0
owner id: 7437
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The part BBa_J04500 was used as a template for the creation of this new part. The -35 and -10 promoter elements were converted through mutagenesis into AflII and ClaI (respectively) restriction sites. A double restriction digest was performed and a T7 promoter was introduced in the form of primers.
mutable notes: We wanted a T7 promoter that could be regulated with the lac system.
mutable provenance: The part BBa_J04500 was used as a template for the creation of this new part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K345667
sequence annotation: B0034
sequence annotation: LacI binding site
sequence annotation: end of LacI coding region (inactive)
sequence annotation: CAP binding site
sequence annotation: ClaI restriction site
sequence annotation: AflII restriction site
sequence: https://synbiohub.org/public/igem/BBa_K345667_sequence/1

BBa_K345667
type: SequenceAnnotation
persistent identity: annotation2114591
display id: annotation2114591
version: 1
top level: BBa_K345667
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K345667/annotation2114591/range2114591/1

https://synbiohub.org/public/igem/BBa_K345667/annotation2114591/range2114591/1
type: Range
persistent identity: range2114591
display id: range2114591
version: 1
direction: direction/reverse
top level: BBa_K345667
start: 1
end: 220
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2114596
display id: annotation2114596
version: 1
top level: BBa_K345667
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K345667/annotation2114596/range2114596/1

https://synbiohub.org/public/igem/BBa_K345667/annotation2114596/range2114596/1
type: Range
persistent identity: range2114596
display id: range2114596
version: 1
direction: direction/forward
top level: BBa_K345667
start: 209
end: 220
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2114593
display id: annotation2114593
version: 1
top level: BBa_K345667
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K345667/annotation2114593/range2114593/1

https://synbiohub.org/public/igem/BBa_K345667/annotation2114593/range2114593/1
type: Range
persistent identity: range2114593
display id: range2114593
version: 1
direction: direction/forward
top level: BBa_K345667
start: 164
end: 198
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2114590
display id: annotation2114590
version: 1
top level: BBa_K345667
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K345667/annotation2114590/range2114590/1

https://synbiohub.org/public/igem/BBa_K345667/annotation2114590/range2114590/1
type: Range
persistent identity: range2114590
display id: range2114590
version: 1
direction: direction/forward
top level: BBa_K345667
start: 1
end: 85
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2114592
display id: annotation2114592
version: 1
top level: BBa_K345667
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K345667/annotation2114592/range2114592/1

https://synbiohub.org/public/igem/BBa_K345667/annotation2114592/range2114592/1
type: Range
persistent identity: range2114592
display id: range2114592
version: 1
direction: direction/forward
top level: BBa_K345667
start: 86
end: 127
orientation: inline

ClaI restriction site
type: SequenceAnnotation
persistent identity: annotation2114595
display id: annotation2114595
version: 1
top level: BBa_K345667
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K345667/annotation2114595/range2114595/1

https://synbiohub.org/public/igem/BBa_K345667/annotation2114595/range2114595/1
type: Range
persistent identity: range2114595
display id: range2114595
version: 1
direction: direction/forward
top level: BBa_K345667
start: 156
end: 161
orientation: inline

AflII restriction site
type: SequenceAnnotation
persistent identity: annotation2114594
display id: annotation2114594
version: 1
top level: BBa_K345667
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K345667/annotation2114594/range2114594/1

https://synbiohub.org/public/igem/BBa_K345667/annotation2114594/range2114594/1
type: Range
persistent identity: range2114594
display id: range2114594
version: 1
direction: direction/forward
top level: BBa_K345667
start: 136
end: 141
orientation: inline

BBa_K215000
type: ComponentDefinition
persistent identity: BBa K215000
display id: BBa_K215000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011+B0034, strong IPTG-inducible promoter with strong RBS.
creator: Chris Eiben
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _320_
m user id: 0
owner id: 2811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part contains the strong, IPTG-inducible promoter R0011, combined with the strong RBS B0034. This part can be used as a strong protein expression system when combined with a protein coding sequence.
mutable notes: This part should offer greater levels of expression when compared to similar parts using the natural lac promoter (R0010).
mutable provenance: existing registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0011
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K215000_sequence/1

BBa_R0011
type: Component
persistent identity: component2027435
display id: component2027435
version: 1
top level: BBa_K215000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component2027441
display id: component2027441
version: 1
top level: BBa_K215000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2027441
display id: annotation2027441
version: 1
top level: BBa_K215000
location: https://synbiohub.org/public/igem/BBa_K215000/annotation2027441/range2027441/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K215000/annotation2027441/range2027441/1
type: Range
persistent identity: range2027441
display id: range2027441
version: 1
direction: direction/reverse
top level: BBa_K215000
start: 64
end: 75
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2027435
display id: annotation2027435
version: 1
top level: BBa_K215000
location: https://synbiohub.org/public/igem/BBa_K215000/annotation2027435/range2027435/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K215000/annotation2027435/range2027435/1
type: Range
persistent identity: range2027435
display id: range2027435
version: 1
direction: direction/forward
top level: BBa_K215000
start: 1
end: 54
orientation: inline

pSBBs4S
type: ComponentDefinition
persistent identity: BBa K823024
display id: BBa_K823024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs4S-Pxyl: Integrative expression vector for Bacillus subtilis</i
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
mutable provenance: This part is an igem compatible version of the Bacillus expression vector pXT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: thrB
sequence annotation: bla
sequence annotation: spc
sequence annotation: hom
sequence annotation: 'thrC
sequence annotation: Pxly region
sequence annotation: thrC'
sequence: https://synbiohub.org/public/igem/BBa_K823024_sequence/1

thrB
type: SequenceAnnotation
persistent identity: annotation2180242
display id: annotation2180242
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180242/range2180242/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180242/range2180242/1
type: Range
persistent identity: range2180242
display id: range2180242
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1453
end: 1863
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180243
display id: annotation2180243
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180243/range2180243/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180243/range2180243/1
type: Range
persistent identity: range2180243
display id: range2180243
version: 1
direction: direction/reverse
top level: pSBBs4S
start: 2716
end: 3576
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2180240
display id: annotation2180240
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180240/range2180240/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180240/range2180240/1
type: Range
persistent identity: range2180240
display id: range2180240
version: 1
direction: direction/forward
top level: pSBBs4S
start: 211
end: 885
orientation: inline

hom
type: SequenceAnnotation
persistent identity: annotation2180244
display id: annotation2180244
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180244/range2180244/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180244/range2180244/1
type: Range
persistent identity: range2180244
display id: range2180244
version: 1
direction: direction/forward
top level: pSBBs4S
start: 3731
end: 4033
orientation: inline

'thrC
type: SequenceAnnotation
persistent identity: annotation2180241
display id: annotation2180241
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180241/range2180241/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180241/range2180241/1
type: Range
persistent identity: range2180241
display id: range2180241
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1028
end: 1456
orientation: inline

Pxly region
type: SequenceAnnotation
persistent identity: annotation2180246
display id: annotation2180246
version: 1
top level: pSBBs4S
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180246/range2180246/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180246/range2180246/1
type: Range
persistent identity: range2180246
display id: range2180246
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4539
end: 4763
orientation: inline

thrC'
type: SequenceAnnotation
persistent identity: annotation2180245
display id: annotation2180245
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180245/range2180245/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180245/range2180245/1
type: Range
persistent identity: range2180245
display id: range2180245
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4033
end: 4572
orientation: inline

xyl+efe
type: ComponentDefinition
persistent identity: BBa K1065204
display id: BBa_K1065204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Efe+Bba_B0015 in BBa_K823024 (pXyl)
creator: Viola Valentini
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1373_
m user id: 0
owner id: 15998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pXyl vector that has been sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.
mutable notes: ===Design Notes=== The coding sequence was taken from Pseudomonas syringae pv. phaseolicola PK2. We firstly optimized the codons for both B. subtilis and E. coli and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly. GAATTCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGCACC... ...TCGACCGGTTAATACTAGTAGCGGCCGCTGCAG
mutable provenance: This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K823024
component: BBa_K1065002
sequence annotation: BBa_K823024
sequence annotation: BBa_K1065002
sequence: https://synbiohub.org/public/igem/BBa_K1065204_sequence/1

BBa_K823024
type: Component
persistent identity: component2334267
display id: component2334267
version: 1
top level: xyl+efe
access: public
definition: pSBBs4S

BBa_K1065002
type: Component
persistent identity: component2334259
display id: component2334259
version: 1
top level: xyl+efe
access: public
definition: https://synbiohub.org/public/igem/BBa_K1065002/1

BBa_K823024
type: SequenceAnnotation
persistent identity: annotation2334267
display id: annotation2334267
version: 1
top level: xyl+efe
location: https://synbiohub.org/public/igem/BBa_K1065204/annotation2334267/range2334267/1
component: BBa_K823024

https://synbiohub.org/public/igem/BBa_K1065204/annotation2334267/range2334267/1
type: Range
persistent identity: range2334267
display id: range2334267
version: 1
direction: direction/reverse
top level: xyl+efe
start: 1224
end: 6017
orientation: inline

BBa_K1065002
type: SequenceAnnotation
persistent identity: annotation2334259
display id: annotation2334259
version: 1
top level: xyl+efe
location: https://synbiohub.org/public/igem/BBa_K1065204/annotation2334259/range2334259/1
component: BBa_K1065002

https://synbiohub.org/public/igem/BBa_K1065204/annotation2334259/range2334259/1
type: Range
persistent identity: range2334259
display id: range2334259
version: 1
direction: direction/reverse
top level: xyl+efe
start: 1
end: 1215
orientation: inline

BBa_K1680026
type: ComponentDefinition
persistent identity: BBa K1680026
display id: BBa_K1680026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pETUE
creator: Katharina Sporbeck
created: 2015-09-17T11:00:00Z
modified: 2015-10-28T03:58:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Expression Plasmid coding for His-tag, also containing a thrombine cleavage site between coding region and Histag, so that His-tag can be cleaved off. Vecotr opening with PstI/XmaI, clone part into it with PstI/NgoMIV. Ampicillin resistance.
mutable notes: very strong RBS 6xHis-Tag Thromin-cleavage-site NgoMIV-compatible restriction site for in-frame insertion
mutable provenance: pET-Vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: thrombin cleavage site
sequence annotation: RBS.1 strong
sequence annotation: T7 promoter
sequence annotation: 6x His-tag
sequence annotation: Lac operator
sequence annotation: ori
sequence annotation: tet promoter
sequence annotation: T7 terminator
sequence annotation: f1 ori
sequence annotation: AmpR
sequence annotation: lac operator
sequence annotation: rrnB T1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1680026_sequence/1

thrombin cleavage site
type: SequenceAnnotation
persistent identity: annotation2472237
display id: annotation2472237
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472237/range2472237/1

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type: Range
persistent identity: range2472237
display id: range2472237
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 104
end: 121
orientation: inline

RBS.1 strong
type: SequenceAnnotation
persistent identity: annotation2472195
display id: annotation2472195
version: 1
top level: BBa_K1680026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472195/range2472195/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472195/range2472195/1
type: Range
persistent identity: range2472195
display id: range2472195
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 53
end: 67
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2472193
display id: annotation2472193
version: 1
top level: BBa_K1680026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472193/range2472193/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472193/range2472193/1
type: Range
persistent identity: range2472193
display id: range2472193
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 1
end: 19
orientation: inline

6x His-tag
type: SequenceAnnotation
persistent identity: annotation2472216
display id: annotation2472216
version: 1
top level: BBa_K1680026
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472216/range2472216/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472216/range2472216/1
type: Range
persistent identity: range2472216
display id: range2472216
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 77
end: 94
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2472194
display id: annotation2472194
version: 1
top level: BBa_K1680026
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472194/range2472194/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472194/range2472194/1
type: Range
persistent identity: range2472194
display id: range2472194
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 20
end: 44
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2472305
display id: annotation2472305
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472305/range2472305/1

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type: Range
persistent identity: range2472305
display id: range2472305
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 2290
end: 2878
orientation: inline

tet promoter
type: SequenceAnnotation
persistent identity: annotation2472238
display id: annotation2472238
version: 1
top level: BBa_K1680026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472238/range2472238/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472238/range2472238/1
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persistent identity: range2472238
display id: range2472238
version: 1
direction: direction/reverse
top level: BBa_K1680026
start: 212
end: 240
orientation: inline

T7 terminator
type: SequenceAnnotation
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display id: annotation2472260
version: 1
top level: BBa_K1680026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472260/range2472260/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472260/range2472260/1
type: Range
persistent identity: range2472260
display id: range2472260
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 683
end: 730
orientation: inline

f1 ori
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display id: annotation2472261
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472261/range2472261/1

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persistent identity: range2472261
display id: range2472261
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 767
end: 1222
orientation: inline

AmpR
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persistent identity: annotation2472289
display id: annotation2472289
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472289/range2472289/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472289/range2472289/1
type: Range
persistent identity: range2472289
display id: range2472289
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 1337
end: 2197
orientation: inline

lac operator
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persistent identity: annotation2472328
display id: annotation2472328
version: 1
top level: BBa_K1680026
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472328/range2472328/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472328/range2472328/1
type: Range
persistent identity: range2472328
display id: range2472328
version: 1
direction: direction/forward
top level: BBa_K1680026
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end: 3296
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2472239
display id: annotation2472239
version: 1
top level: BBa_K1680026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472239/range2472239/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472239/range2472239/1
type: Range
persistent identity: range2472239
display id: range2472239
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 410
end: 496
orientation: inline

pSB4K50
type: ComponentDefinition
persistent identity: BBa K1362097
display id: BBa_K1362097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick expression backbone carrying Kan resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00000
component: BBa_B0053
component: BBa_B0062
component: BBa_B0042
component: BBa_P1007
component: BBa_B0055
component: BBa_G00102
component: BBa_B0054
component: BBa_I50041
component: BBa_K1362463
component: BBa_B0043
component: BBa_B0044
component: BBa_G0001
component: BBa_G00100
component: BBa_G00001
component: BBa_G0005
component: BBa_G0006
sequence annotation: BBa_G0006
sequence annotation: BBa_B0043
sequence annotation: BBa_K1362463
sequence annotation: BBa_B0062
sequence annotation: BBa_G00102
sequence annotation: BBa_G00100
sequence annotation: BBa_G0001
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_P1007
sequence annotation: BBa_B0042
sequence annotation: BBa_B0055
sequence annotation: BBa_G00000
sequence annotation: BBa_G0005
sequence annotation: BBa_I50041
sequence annotation: BBa_G00001
sequence annotation: BBa_B0054
sequence: https://synbiohub.org/public/igem/BBa_K1362097_sequence/1

BBa_G00000
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persistent identity: component2410017
display id: component2410017
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top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0053
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display id: component2409991
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0062
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persistent identity: component2409956
display id: component2409956
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
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display id: component2409949
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1007
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persistent identity: component2409961
display id: component2409961
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1007/1

BBa_B0055
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display id: component2409996
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top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00102
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display id: component2409954
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0054
type: Component
persistent identity: component2409952
display id: component2409952
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_I50041
type: Component
persistent identity: component2409988
display id: component2409988
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top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50041/1

BBa_K1362463
type: Component
persistent identity: component2410012
display id: component2410012
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_B0043
type: Component
persistent identity: component2410008
display id: component2410008
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
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persistent identity: component2410003
display id: component2410003
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
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display id: component2409942
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G0001
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display id: component2409962
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0001/1

BBa_G00100
type: Component
persistent identity: component2409993
display id: component2409993
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00001
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display id: component2409937
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G0005
type: Component
persistent identity: component2409957
display id: component2409957
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0005/1

BBa_G0006
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persistent identity: component2409989
display id: component2409989
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0006/1

BBa_G0006
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persistent identity: annotation2409989
display id: annotation2409989
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409989/range2409989/1
component: BBa_G0006

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display id: range2409989
version: 1
direction: direction/reverse
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end: 3202
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2410008
display id: annotation2410008
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410008/range2410008/1
component: BBa_B0043

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display id: range2410008
version: 1
direction: direction/reverse
top level: pSB4K50
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orientation: inline

BBa_K1362463
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display id: annotation2410012
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top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
component: BBa_K1362463

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persistent identity: range2410012
display id: range2410012
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direction: direction/reverse
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end: 3448
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BBa_B0062
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persistent identity: annotation2409956
display id: annotation2409956
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direction: direction/reverse
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BBa_G00102
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direction: direction/reverse
top level: pSB4K50
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BBa_G00100
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persistent identity: annotation2409993
display id: annotation2409993
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top level: pSB4K50
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component: BBa_G00100

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persistent identity: range2409993
display id: range2409993
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direction: direction/reverse
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orientation: inline

BBa_G0001
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persistent identity: range2409962
display id: range2409962
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direction: direction/reverse
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BBa_B0053
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display id: annotation2409991
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persistent identity: range2409991
display id: range2409991
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direction: direction/reverse
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orientation: inline

BBa_B0044
type: SequenceAnnotation
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display id: annotation2409942
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persistent identity: range2409942
display id: range2409942
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direction: direction/reverse
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BBa_P1007
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display id: annotation2409961
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BBa_B0042
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persistent identity: range2410003
display id: range2410003
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BBa_B0042
type: SequenceAnnotation
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persistent identity: range2409949
display id: range2409949
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BBa_B0055
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persistent identity: range2409996
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direction: direction/reverse
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BBa_G00000
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component: BBa_G00000

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type: Range
persistent identity: range2410017
display id: range2410017
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BBa_G0005
type: SequenceAnnotation
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persistent identity: range2409957
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BBa_I50041
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BBa_G00001
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persistent identity: range2409937
display id: range2409937
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direction: direction/reverse
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BBa_B0054
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persistent identity: range2409952
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BBa_J13023
type: ComponentDefinition
persistent identity: BBa J13023
display id: BBa_J13023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL+LuxR dependent POPS/RIPS generator
creator: Jeff Tabor
created: 2005-06-28T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: produces pops and rips in the presence of luxR/3OC6HSL and the absence of lambda cI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0034
component: BBa_R0065
sequence annotation: BBa_B0034
sequence annotation: BBa_R0065
sequence: https://synbiohub.org/public/igem/BBa_J13023_sequence/1

BBa_B0034
type: Component
persistent identity: component1556583
display id: component1556583
version: 1
top level: BBa_J13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0065
type: Component
persistent identity: component1556571
display id: component1556571
version: 1
top level: BBa_J13023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1556583
display id: annotation1556583
version: 1
top level: BBa_J13023
location: https://synbiohub.org/public/igem/BBa_J13023/annotation1556583/range1556583/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13023/annotation1556583/range1556583/1
type: Range
persistent identity: range1556583
display id: range1556583
version: 1
direction: direction/forward
top level: BBa_J13023
start: 106
end: 117
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1556571
display id: annotation1556571
version: 1
top level: BBa_J13023
location: https://synbiohub.org/public/igem/BBa_J13023/annotation1556571/range1556571/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_J13023/annotation1556571/range1556571/1
type: Range
persistent identity: range1556571
display id: range1556571
version: 1
direction: direction/forward
top level: BBa_J13023
start: 1
end: 97
orientation: inline

spac+efe
type: ComponentDefinition
persistent identity: BBa K1065203
display id: BBa_K1065203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Efe+Bba_B0015 in pSpac (BBa_K823026)
creator: Viola Valentini
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1373_
m user id: 0
owner id: 15998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pSpac vector that has benn sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.
mutable notes: The coding sequence was taken from Pseudomonas syringae pv. phaseolicola PK2. We firstly optimized the codons for both B. subtilis and E. coli and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly.
mutable provenance: This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1065002
component: BBa_K823026
sequence annotation: BBa_K1065002
sequence annotation: BBa_K823026
sequence: https://synbiohub.org/public/igem/BBa_K1065203_sequence/1

BBa_K1065002
type: Component
persistent identity: component2334235
display id: component2334235
version: 1
top level: spac+efe
access: public
definition: https://synbiohub.org/public/igem/BBa_K1065002/1

BBa_K823026
type: Component
persistent identity: component2334243
display id: component2334243
version: 1
top level: spac+efe
access: public
definition: pSBBs0K

BBa_K1065002
type: SequenceAnnotation
persistent identity: annotation2334235
display id: annotation2334235
version: 1
top level: spac+efe
location: https://synbiohub.org/public/igem/BBa_K1065203/annotation2334235/range2334235/1
component: BBa_K1065002

https://synbiohub.org/public/igem/BBa_K1065203/annotation2334235/range2334235/1
type: Range
persistent identity: range2334235
display id: range2334235
version: 1
direction: direction/reverse
top level: spac+efe
start: 1
end: 1215
orientation: inline

BBa_K823026
type: SequenceAnnotation
persistent identity: annotation2334243
display id: annotation2334243
version: 1
top level: spac+efe
location: https://synbiohub.org/public/igem/BBa_K1065203/annotation2334243/range2334243/1
component: BBa_K823026

https://synbiohub.org/public/igem/BBa_K1065203/annotation2334243/range2334243/1
type: Range
persistent identity: range2334243
display id: range2334243
version: 1
direction: direction/reverse
top level: spac+efe
start: 1224
end: 9512
orientation: inline

BBa_K801004
type: ComponentDefinition
persistent identity: BBa K801004
display id: BBa_K801004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM104 yeast shuttle vector with GAL1 promoter
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is based on the plasmid pTUM100 (BBa_K801000). The galactose-inducible GAL1 yeast promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed, making this plasmid RFC25-compatible.
mutable provenance: Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: PGAL1
sequence annotation: 2 ?? origin
sequence annotation: URA3
sequence annotation: f1 origin
sequence annotation: T7 promoter/priming site
sequence annotation: CYC1 transcription terminator
sequence annotation: pUC1 origin
sequence annotation: Ampicillin
sequence: https://synbiohub.org/public/igem/BBa_K801004_sequence/1

PGAL1
type: SequenceAnnotation
persistent identity: annotation2213076
display id: annotation2213076
version: 1
top level: BBa_K801004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213076/range2213076/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213076/range2213076/1
type: Range
persistent identity: range2213076
display id: range2213076
version: 1
direction: direction/forward
top level: BBa_K801004
start: 5293
end: 5743
orientation: inline

2 ?? origin
type: SequenceAnnotation
persistent identity: annotation2213074
display id: annotation2213074
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213074/range2213074/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213074/range2213074/1
type: Range
persistent identity: range2213074
display id: range2213074
version: 1
direction: direction/forward
top level: BBa_K801004
start: 3281
end: 4752
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2213073
display id: annotation2213073
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213073/range2213073/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213073/range2213073/1
type: Range
persistent identity: range2213073
display id: range2213073
version: 1
direction: direction/forward
top level: BBa_K801004
start: 2170
end: 3277
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2213075
display id: annotation2213075
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213075/range2213075/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213075/range2213075/1
type: Range
persistent identity: range2213075
display id: range2213075
version: 1
direction: direction/forward
top level: BBa_K801004
start: 4820
end: 5275
orientation: inline

T7 promoter/priming site
type: SequenceAnnotation
persistent identity: annotation2213077
display id: annotation2213077
version: 1
top level: BBa_K801004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213077/range2213077/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213077/range2213077/1
type: Range
persistent identity: range2213077
display id: range2213077
version: 1
direction: direction/forward
top level: BBa_K801004
start: 5767
end: 5786
orientation: inline

CYC1 transcription terminator
type: SequenceAnnotation
persistent identity: annotation2213070
display id: annotation2213070
version: 1
top level: BBa_K801004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213070/range2213070/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213070/range2213070/1
type: Range
persistent identity: range2213070
display id: range2213070
version: 1
direction: direction/forward
top level: BBa_K801004
start: 44
end: 292
orientation: inline

pUC1 origin
type: SequenceAnnotation
persistent identity: annotation2213071
display id: annotation2213071
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213071/range2213071/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213071/range2213071/1
type: Range
persistent identity: range2213071
display id: range2213071
version: 1
direction: direction/forward
top level: BBa_K801004
start: 474
end: 1147
orientation: inline

Ampicillin
type: SequenceAnnotation
persistent identity: annotation2213072
display id: annotation2213072
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213072/range2213072/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213072/range2213072/1
type: Range
persistent identity: range2213072
display id: range2213072
version: 1
direction: direction/forward
top level: BBa_K801004
start: 1292
end: 2152
orientation: inline

pSBBs0K
type: ComponentDefinition
persistent identity: BBa K823026
display id: BBa_K823026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs0K-Pspac (replicative Bacillus subtilis expression vector; IPTG inducible
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has a ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis. The multiple cloning site is downstream of a Pspac promoter, that is inducible with IPTG. (Isopropyl-β-D-thiogalactopyranosid). This BioBrick is part of the LMU 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli. It can be used as an inducible expression vector in B. subtilis.
mutable provenance: This part is an igem compatible version of the Bacillus expression vector pDG148
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: bla
sequence annotation: ble
sequence annotation: lacY
sequence annotation: lacI
sequence annotation: Pspac
sequence annotation: kan
sequence annotation: repU
sequence: https://synbiohub.org/public/igem/BBa_K823026_sequence/1

bla
type: SequenceAnnotation
persistent identity: annotation2180257
display id: annotation2180257
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180257/range2180257/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180257/range2180257/1
type: Range
persistent identity: range2180257
display id: range2180257
version: 1
direction: direction/reverse
top level: pSBBs0K
start: 2609
end: 3469
orientation: inline

ble
type: SequenceAnnotation
persistent identity: annotation2180260
display id: annotation2180260
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180260/range2180260/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180260/range2180260/1
type: Range
persistent identity: range2180260
display id: range2180260
version: 1
direction: direction/forward
top level: pSBBs0K
start: 6250
end: 6648
orientation: inline

lacY
type: SequenceAnnotation
persistent identity: annotation2180261
display id: annotation2180261
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180261/range2180261/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180261/range2180261/1
type: Range
persistent identity: range2180261
display id: range2180261
version: 1
direction: direction/reverse
top level: pSBBs0K
start: 6906
end: 7871
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2180256
display id: annotation2180256
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180256/range2180256/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180256/range2180256/1
type: Range
persistent identity: range2180256
display id: range2180256
version: 1
direction: direction/forward
top level: pSBBs0K
start: 271
end: 1353
orientation: inline

Pspac
type: SequenceAnnotation
persistent identity: annotation2180262
display id: annotation2180262
version: 1
top level: pSBBs0K
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180262/range2180262/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180262/range2180262/1
type: Range
persistent identity: range2180262
display id: range2180262
version: 1
direction: direction/forward
top level: pSBBs0K
start: 8193
end: 8258
orientation: inline

kan
type: SequenceAnnotation
persistent identity: annotation2180259
display id: annotation2180259
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180259/range2180259/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180259/range2180259/1
type: Range
persistent identity: range2180259
display id: range2180259
version: 1
direction: direction/forward
top level: pSBBs0K
start: 5257
end: 6027
orientation: inline

repU
type: SequenceAnnotation
persistent identity: annotation2180258
display id: annotation2180258
version: 1
top level: pSBBs0K
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180258/range2180258/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180258/range2180258/1
type: Range
persistent identity: range2180258
display id: range2180258
version: 1
direction: direction/forward
top level: pSBBs0K
start: 4093
end: 5097
orientation: inline

pSB4C50
type: ComponentDefinition
persistent identity: BBa K1362096
display id: BBa_K1362096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4C50: Low(?) copy BioBrick cloning/expression backbone carrying Cm resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0053
component: BBa_B0042
component: BBa_G0002
component: BBa_B0044
component: BBa_B0062
component: BBa_G0003
component: BBa_G00000
component: BBa_B0043
component: BBa_B0055
component: BBa_G0004
component: BBa_G00102
component: BBa_G00001
component: BBa_I50042
component: BBa_G00100
component: BBa_P1004
component: BBa_B0054
component: BBa_K1362463
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_G00102
sequence annotation: BBa_G00100
sequence annotation: BBa_G0003
sequence annotation: BBa_B0042
sequence annotation: BBa_B0055
sequence annotation: BBa_B0054
sequence annotation: BBa_I50042
sequence annotation: BBa_G00001
sequence annotation: BBa_G0004
sequence annotation: BBa_B0062
sequence annotation: BBa_G0002
sequence annotation: BBa_K1362463
sequence annotation: BBa_P1004
sequence annotation: BBa_G00000
sequence annotation: BBa_B0043
sequence: https://synbiohub.org/public/igem/BBa_K1362096_sequence/1

BBa_B0053
type: Component
persistent identity: component2409906
display id: component2409906
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0042
type: Component
persistent identity: component2409864
display id: component2409864
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: Component
persistent identity: component2409901
display id: component2409901
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0044
type: Component
persistent identity: component2409857
display id: component2409857
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409871
display id: component2409871
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0003
type: Component
persistent identity: component2409872
display id: component2409872
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00000
type: Component
persistent identity: component2409932
display id: component2409932
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0043
type: Component
persistent identity: component2409923
display id: component2409923
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: Component
persistent identity: component2409911
display id: component2409911
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component2409918
display id: component2409918
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0004
type: Component
persistent identity: component2409904
display id: component2409904
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00102
type: Component
persistent identity: component2409869
display id: component2409869
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G00001
type: Component
persistent identity: component2409852
display id: component2409852
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_I50042
type: Component
persistent identity: component2409900
display id: component2409900
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_G00100
type: Component
persistent identity: component2409908
display id: component2409908
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_P1004
type: Component
persistent identity: component2409903
display id: component2409903
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0054
type: Component
persistent identity: component2409867
display id: component2409867
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_K1362463
type: Component
persistent identity: component2409927
display id: component2409927
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409906
display id: annotation2409906
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
type: Range
persistent identity: range2409906
display id: range2409906
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3093
end: 3164
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409857
display id: annotation2409857
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
component: BBa_B0044

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type: Range
persistent identity: range2409857
display id: range2409857
version: 1
direction: direction/reverse
top level: pSB4C50
start: 30
end: 42
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409869
display id: annotation2409869
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
component: BBa_G00102

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type: Range
persistent identity: range2409869
display id: range2409869
version: 1
direction: direction/reverse
top level: pSB4C50
start: 148
end: 167
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409908
display id: annotation2409908
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
component: BBa_G00100

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type: Range
persistent identity: range2409908
display id: range2409908
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3173
end: 3192
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409872
display id: annotation2409872
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
component: BBa_G0003

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type: Range
persistent identity: range2409872
display id: range2409872
version: 1
direction: direction/reverse
top level: pSB4C50
start: 225
end: 231
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409864
display id: annotation2409864
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
component: BBa_B0042

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type: Range
persistent identity: range2409864
display id: range2409864
version: 1
direction: direction/reverse
top level: pSB4C50
start: 51
end: 62
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409911
display id: annotation2409911
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
component: BBa_B0055

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type: Range
persistent identity: range2409911
display id: range2409911
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3201
end: 3278
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409867
display id: annotation2409867
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
component: BBa_B0054

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type: Range
persistent identity: range2409867
display id: range2409867
version: 1
direction: direction/reverse
top level: pSB4C50
start: 71
end: 139
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409900
display id: annotation2409900
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
component: BBa_I50042

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type: Range
persistent identity: range2409900
display id: range2409900
version: 1
direction: direction/reverse
top level: pSB4C50
start: 240
end: 2276
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409852
display id: annotation2409852
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
component: BBa_G00001

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type: Range
persistent identity: range2409852
display id: range2409852
version: 1
direction: direction/reverse
top level: pSB4C50
start: 1
end: 21
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409918
display id: annotation2409918
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
component: BBa_B0042

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type: Range
persistent identity: range2409918
display id: range2409918
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3287
end: 3298
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409904
display id: annotation2409904
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
type: Range
persistent identity: range2409904
display id: range2409904
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3078
end: 3084
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409871
display id: annotation2409871
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
type: Range
persistent identity: range2409871
display id: range2409871
version: 1
direction: direction/reverse
top level: pSB4C50
start: 176
end: 216
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409901
display id: annotation2409901
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
type: Range
persistent identity: range2409901
display id: range2409901
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2285
end: 2292
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409927
display id: annotation2409927
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
type: Range
persistent identity: range2409927
display id: range2409927
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3328
end: 3378
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2409903
display id: annotation2409903
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
component: BBa_P1004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
type: Range
persistent identity: range2409903
display id: range2409903
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2301
end: 3069
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409932
display id: annotation2409932
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
component: BBa_G00000

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type: Range
persistent identity: range2409932
display id: range2409932
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3387
end: 3408
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409923
display id: annotation2409923
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
type: Range
persistent identity: range2409923
display id: range2409923
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3307
end: 3319
orientation: inline

pSB1A30
type: ComponentDefinition
persistent identity: BBa K1362091
display id: BBa_K1362091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A30: High copy BioBrick cloning/expression backbone carrying Amp resitance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: end of terminator
sequence annotation: VF2
sequence annotation: prefix
sequence annotation: rep(pMB1) (rev)
sequence annotation: ORI
sequence annotation: suffix
sequence annotation: lacO reg
sequence annotation: terminator stem loop
sequence annotation: VR
sequence annotation: ampR (rev)
sequence annotation: T7 promotor
sequence annotation: E. coli his operon terminator
sequence: https://synbiohub.org/public/igem/BBa_K1362091_sequence/1

end of terminator
type: SequenceAnnotation
persistent identity: annotation2404609
display id: annotation2404609
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1
type: Range
persistent identity: range2404609
display id: range2404609
version: 1
direction: direction/reverse
top level: pSB1A30
start: 2094
end: 2097
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2404608
display id: annotation2404608
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1
type: Range
persistent identity: range2404608
display id: range2404608
version: 1
direction: direction/forward
top level: pSB1A30
start: 2018
end: 2037
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2404632
display id: annotation2404632
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1
type: Range
persistent identity: range2404632
display id: range2404632
version: 1
direction: direction/forward
top level: pSB1A30
start: 2185
end: 2206
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2404605
display id: annotation2404605
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1
type: Range
persistent identity: range2404605
display id: range2404605
version: 1
direction: direction/reverse
top level: pSB1A30
start: 261
end: 875
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2404606
display id: annotation2404606
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1
type: Range
persistent identity: range2404606
display id: range2404606
version: 1
direction: direction/forward
top level: pSB1A30
start: 276
end: 276
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2404600
display id: annotation2404600
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1
type: Range
persistent identity: range2404600
display id: range2404600
version: 1
direction: direction/forward
top level: pSB1A30
start: 1
end: 21
orientation: inline

lacO reg
type: SequenceAnnotation
persistent identity: annotation2404631
display id: annotation2404631
version: 1
top level: pSB1A30
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1
type: Range
persistent identity: range2404631
display id: range2404631
version: 1
direction: direction/forward
top level: pSB1A30
start: 2157
end: 2184
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404603
display id: annotation2404603
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1
type: Range
persistent identity: range2404603
display id: range2404603
version: 1
direction: direction/forward
top level: pSB1A30
start: 30
end: 64
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2404602
display id: annotation2404602
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1
type: Range
persistent identity: range2404602
display id: range2404602
version: 1
direction: direction/reverse
top level: pSB1A30
start: 157
end: 176
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation2404607
display id: annotation2404607
version: 1
top level: pSB1A30
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1
type: Range
persistent identity: range2404607
display id: range2404607
version: 1
direction: direction/reverse
top level: pSB1A30
start: 1035
end: 1895
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2404611
display id: annotation2404611
version: 1
top level: pSB1A30
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1
type: Range
persistent identity: range2404611
display id: range2404611
version: 1
direction: direction/forward
top level: pSB1A30
start: 2139
end: 2157
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2404601
display id: annotation2404601
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1
type: Range
persistent identity: range2404601
display id: range2404601
version: 1
direction: direction/forward
top level: pSB1A30
start: 22
end: 93
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404610
display id: annotation2404610
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1
type: Range
persistent identity: range2404610
display id: range2404610
version: 1
direction: direction/forward
top level: pSB1A30
start: 2107
end: 2126
orientation: inline

BBa_K081006
type: ComponentDefinition
persistent identity: BBa K081006
display id: BBa_K081006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plambda promoter and RBS
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Plambda is a repressible promoter, cI regulated (BBa_C0051). Without cI, the promoter is ON with strong activity. RBS is strong (efficiency=0.6)
mutable notes: We used BioBrick Standard Assembly
mutable provenance: Plambda: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross RBS:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0051
component: BBa_B0030
sequence annotation: BBa_R0051
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081006_sequence/1

BBa_R0051
type: Component
persistent identity: component1981868
display id: component1981868
version: 1
top level: BBa_K081006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0030
type: Component
persistent identity: component1981873
display id: component1981873
version: 1
top level: BBa_K081006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1981868
display id: annotation1981868
version: 1
top level: BBa_K081006
location: https://synbiohub.org/public/igem/BBa_K081006/annotation1981868/range1981868/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K081006/annotation1981868/range1981868/1
type: Range
persistent identity: range1981868
display id: range1981868
version: 1
direction: direction/forward
top level: BBa_K081006
start: 1
end: 49
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1981873
display id: annotation1981873
version: 1
top level: BBa_K081006
location: https://synbiohub.org/public/igem/BBa_K081006/annotation1981873/range1981873/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081006/annotation1981873/range1981873/1
type: Range
persistent identity: range1981873
display id: range1981873
version: 1
direction: direction/forward
top level: BBa_K081006
start: 58
end: 72
orientation: inline

BBa_S03511
type: ComponentDefinition
persistent identity: BBa S03511
display id: BBa_S03511
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac : RBS
creator: Davidson and Missouri Western
created: 2006-07-20T11:00:00Z
modified: 2015-05-08T01:14:24Z
discontinued: false
dominant: true
experience: experience/None
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0030
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_S03511_sequence/1

BBa_B0030
type: Component
persistent identity: component1886275
display id: component1886275
version: 1
top level: BBa_S03511
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0010
type: Component
persistent identity: component1886267
display id: component1886267
version: 1
top level: BBa_S03511
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1886267
display id: annotation1886267
version: 1
top level: BBa_S03511
location: https://synbiohub.org/public/igem/BBa_S03511/annotation1886267/range1886267/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_S03511/annotation1886267/range1886267/1
type: Range
persistent identity: range1886267
display id: range1886267
version: 1
direction: direction/forward
top level: BBa_S03511
start: 1
end: 200
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1886275
display id: annotation1886275
version: 1
top level: BBa_S03511
location: https://synbiohub.org/public/igem/BBa_S03511/annotation1886275/range1886275/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_S03511/annotation1886275/range1886275/1
type: Range
persistent identity: range1886275
display id: range1886275
version: 1
direction: direction/forward
top level: BBa_S03511
start: 209
end: 223
orientation: inline

pSB4A50
type: ComponentDefinition
persistent identity: BBa K1362095
display id: BBa_K1362095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A50: Low copy BioBrick cloning/expression backbone carrying Amp resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00100
component: BBa_G0004
component: BBa_G00001
component: BBa_P1002
component: BBa_I50042
component: BBa_B0053
component: BBa_G0003
component: BBa_G00000
component: BBa_B0054
component: BBa_B0042
component: BBa_G0002
component: BBa_B0055
component: BBa_B0044
component: BBa_B0062
component: BBa_K1362463
component: BBa_G00102
component: BBa_B0043
sequence annotation: BBa_G00100
sequence annotation: BBa_P1002
sequence annotation: BBa_B0042
sequence annotation: BBa_I50042
sequence annotation: BBa_B0054
sequence annotation: BBa_B0055
sequence annotation: BBa_G00102
sequence annotation: BBa_G00000
sequence annotation: BBa_B0044
sequence annotation: BBa_B0043
sequence annotation: BBa_B0053
sequence annotation: BBa_G0002
sequence annotation: BBa_G00001
sequence annotation: BBa_G0004
sequence annotation: BBa_K1362463
sequence annotation: BBa_G0003
sequence annotation: BBa_B0062
sequence: https://synbiohub.org/public/igem/BBa_K1362095_sequence/1

BBa_G00100
type: Component
persistent identity: component2409823
display id: component2409823
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G0004
type: Component
persistent identity: component2409819
display id: component2409819
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00001
type: Component
persistent identity: component2409764
display id: component2409764
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_P1002
type: Component
persistent identity: component2409818
display id: component2409818
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_I50042
type: Component
persistent identity: component2409812
display id: component2409812
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0053
type: Component
persistent identity: component2409821
display id: component2409821
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0003
type: Component
persistent identity: component2409784
display id: component2409784
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00000
type: Component
persistent identity: component2409847
display id: component2409847
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0054
type: Component
persistent identity: component2409779
display id: component2409779
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0042
type: Component
persistent identity: component2409776
display id: component2409776
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: Component
persistent identity: component2409813
display id: component2409813
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0055
type: Component
persistent identity: component2409826
display id: component2409826
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component2409833
display id: component2409833
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component2409769
display id: component2409769
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409783
display id: component2409783
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_K1362463
type: Component
persistent identity: component2409842
display id: component2409842
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_G00102
type: Component
persistent identity: component2409781
display id: component2409781
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0043
type: Component
persistent identity: component2409838
display id: component2409838
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409823
display id: annotation2409823
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
type: Range
persistent identity: range2409823
display id: range2409823
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3251
end: 3270
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2409818
display id: annotation2409818
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
component: BBa_P1002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
type: Range
persistent identity: range2409818
display id: range2409818
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2229
end: 3171
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409776
display id: annotation2409776
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
type: Range
persistent identity: range2409776
display id: range2409776
version: 1
direction: direction/reverse
top level: pSB4A50
start: 35
end: 46
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409812
display id: annotation2409812
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
type: Range
persistent identity: range2409812
display id: range2409812
version: 1
direction: direction/reverse
top level: pSB4A50
start: 184
end: 2220
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409779
display id: annotation2409779
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
type: Range
persistent identity: range2409779
display id: range2409779
version: 1
direction: direction/reverse
top level: pSB4A50
start: 47
end: 115
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409826
display id: annotation2409826
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
type: Range
persistent identity: range2409826
display id: range2409826
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3271
end: 3348
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409781
display id: annotation2409781
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
type: Range
persistent identity: range2409781
display id: range2409781
version: 1
direction: direction/reverse
top level: pSB4A50
start: 116
end: 135
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409847
display id: annotation2409847
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
type: Range
persistent identity: range2409847
display id: range2409847
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3425
end: 3446
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409769
display id: annotation2409769
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
type: Range
persistent identity: range2409769
display id: range2409769
version: 1
direction: direction/reverse
top level: pSB4A50
start: 22
end: 34
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409838
display id: annotation2409838
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
type: Range
persistent identity: range2409838
display id: range2409838
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3361
end: 3373
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409821
display id: annotation2409821
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
type: Range
persistent identity: range2409821
display id: range2409821
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3179
end: 3250
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409813
display id: annotation2409813
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
type: Range
persistent identity: range2409813
display id: range2409813
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2221
end: 2228
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409764
display id: annotation2409764
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
type: Range
persistent identity: range2409764
display id: range2409764
version: 1
direction: direction/reverse
top level: pSB4A50
start: 1
end: 21
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409819
display id: annotation2409819
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
type: Range
persistent identity: range2409819
display id: range2409819
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3172
end: 3178
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409842
display id: annotation2409842
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
type: Range
persistent identity: range2409842
display id: range2409842
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3374
end: 3424
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409833
display id: annotation2409833
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
type: Range
persistent identity: range2409833
display id: range2409833
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3349
end: 3360
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409784
display id: annotation2409784
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
type: Range
persistent identity: range2409784
display id: range2409784
version: 1
direction: direction/reverse
top level: pSB4A50
start: 177
end: 183
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409783
display id: annotation2409783
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
type: Range
persistent identity: range2409783
display id: range2409783
version: 1
direction: direction/reverse
top level: pSB4A50
start: 136
end: 176
orientation: inline