//plasmidbackbone/libraryscreening/codingsequence
type: Collection
persistent identity: plasmidbackbone libraryscreening codingsequence
display id: plasmidbackbone_libraryscreening_codingsequence
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J23019
member: BBa_J61003
member: BBa_J23018

BBa_J61003
type: ComponentDefinition
persistent identity: BBa J61003
display id: BBa_J61003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EN-Ptet-rbs-X-[GFP]-SNP "ORF Expresser"
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid for assembly of open reading frame basic parts. A Tet promoter (r0040) and strong ribosome binding site (b0034) lie between the EcoRI and XbaI sites. Insertion of an open reading frame basic part into the XbaI and PstI sites results in constitutive expression but retains basic part utilityin standard assembly.
mutable notes: N/A
mutable provenance: PCR KB001/ca1021R on pSB1A2-J01022 (250 bp, EcoRI/XbaI) sub into pSB1A2-E0040(GFP;DNA-1-5H) (EcoRI/XbaI) Product is pBca1021-E0040 ---- ca1021R Reverse b0034 XbaI AGCCATCTAGAATTTCTCCTCTTTCTCTAG KB001 Forward EcoRI to construct Ptet part-making biobrick plasmid ttctggaattcgcggccgcaactagagccaggcatc
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pBR322
sequence annotation: BBa_R0040 (Ptet)
sequence annotation: BBa_B0034 RBS
sequence annotation: RBS
sequence annotation: ColE1 origin
sequence annotation: rrnB T1 terminator
sequence annotation: BBa_B0015 (terminator)
sequence annotation: EcoRI
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: XbaI
sequence annotation: Ptet promoter
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: NotI
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: BioBrick suffix
sequence: https://synbiohub.org/public/igem/BBa_J61003_sequence/1

pBR322
type: SequenceAnnotation
persistent identity: annotation1975748
display id: annotation1975748
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975748/range1975748/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975748/range1975748/1
type: Range
persistent identity: range1975748
display id: range1975748
version: 1
direction: direction/reverse
top level: BBa_J61003
start: 183
end: 802
orientation: inline

BBa_R0040 (Ptet)
type: SequenceAnnotation
persistent identity: annotation1892278
display id: annotation1892278
version: 1
top level: BBa_J61003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892278/range1892278/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892278/range1892278/1
type: Range
persistent identity: range1892278
display id: range1892278
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2217
end: 2270
orientation: inline

BBa_B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1975755
display id: annotation1975755
version: 1
top level: BBa_J61003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975755/range1975755/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975755/range1975755/1
type: Range
persistent identity: range1975755
display id: range1975755
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2279
end: 2290
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975756
display id: annotation1975756
version: 1
top level: BBa_J61003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975756/range1975756/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975756/range1975756/1
type: Range
persistent identity: range1975756
display id: range1975756
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2279
end: 2290
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1892280
display id: annotation1892280
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892280/range1892280/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892280/range1892280/1
type: Range
persistent identity: range1892280
display id: range1892280
version: 1
direction: direction/forward
top level: BBa_J61003
start: 180
end: 862
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975747
display id: annotation1975747
version: 1
top level: BBa_J61003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975747/range1975747/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975747/range1975747/1
type: Range
persistent identity: range1975747
display id: range1975747
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2091
end: 2134
orientation: inline

BBa_B0015 (terminator)
type: SequenceAnnotation
persistent identity: annotation1892277
display id: annotation1892277
version: 1
top level: BBa_J61003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892277/range1892277/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892277/range1892277/1
type: Range
persistent identity: range1892277
display id: range1892277
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2080
end: 2208
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975750
display id: annotation1975750
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975750/range1975750/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975750/range1975750/1
type: Range
persistent identity: range1975750
display id: range1975750
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2058
end: 2063
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975754
display id: annotation1975754
version: 1
top level: BBa_J61003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975754/range1975754/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975754/range1975754/1
type: Range
persistent identity: range1975754
display id: range1975754
version: 1
direction: direction/reverse
top level: BBa_J61003
start: 79
end: 98
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975752
display id: annotation1975752
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975752/range1975752/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975752/range1975752/1
type: Range
persistent identity: range1975752
display id: range1975752
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2292
end: 2297
orientation: inline

Ptet promoter
type: SequenceAnnotation
persistent identity: annotation1975757
display id: annotation1975757
version: 1
top level: BBa_J61003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975757/range1975757/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975757/range1975757/1
type: Range
persistent identity: range1975757
display id: range1975757
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2217
end: 2270
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1892281
display id: annotation1892281
version: 1
top level: BBa_J61003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892281/range1892281/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892281/range1892281/1
type: Range
persistent identity: range1892281
display id: range1892281
version: 1
direction: direction/reverse
top level: BBa_J61003
start: 957
end: 1817
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975753
display id: annotation1975753
version: 1
top level: BBa_J61003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975753/range1975753/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975753/range1975753/1
type: Range
persistent identity: range1975753
display id: range1975753
version: 1
direction: direction/forward
top level: BBa_J61003
start: 1940
end: 1959
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975751
display id: annotation1975751
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975751/range1975751/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975751/range1975751/1
type: Range
persistent identity: range1975751
display id: range1975751
version: 1
direction: direction/forward
top level: BBa_J61003
start: 2064
end: 2071
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1892279
display id: annotation1892279
version: 1
top level: BBa_J61003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892279/range1892279/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892279/range1892279/1
type: Range
persistent identity: range1892279
display id: range1892279
version: 1
direction: direction/reverse
top level: BBa_J61003
start: 1859
end: 1887
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975749
display id: annotation1975749
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975749/range1975749/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975749/range1975749/1
type: Range
persistent identity: range1975749
display id: range1975749
version: 1
direction: direction/forward
top level: BBa_J61003
start: 1
end: 21
orientation: inline

BBa_J23018
type: ComponentDefinition
persistent identity: BBa J23018
display id: BBa_J23018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"
creator: John Anderson
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Variant of pBca1021 (J61003) with GFP inserted. In practice, it is a E0040 basic part just in a different pSB1A* plasmid.
mutable notes: N/A
mutable provenance: J61003 and E0040
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: EcoRI
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: XbaI
sequence annotation: BBa_R0040 (Ptet)
sequence annotation: NotI
sequence annotation: BBa_B0034
sequence annotation: rrnB T1 terminator
sequence annotation: RBS
sequence annotation: BBa_B0015 terminator
sequence annotation: pBR322 origin
sequence annotation: pMB1 origin
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: BioBrick suffix
sequence annotation: Ptet
sequence: https://synbiohub.org/public/igem/BBa_J23018_sequence/1

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975738
display id: annotation1975738
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975738/range1975738/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975738/range1975738/1
type: Range
persistent identity: range1975738
display id: range1975738
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2058
end: 2063
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1975734
display id: annotation1975734
version: 1
top level: BBa_J23018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975734/range1975734/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975734/range1975734/1
type: Range
persistent identity: range1975734
display id: range1975734
version: 1
direction: direction/reverse
top level: BBa_J23018
start: 957
end: 1817
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975760
display id: annotation1975760
version: 1
top level: BBa_J23018
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975760/range1975760/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975760/range1975760/1
type: Range
persistent identity: range1975760
display id: range1975760
version: 1
direction: direction/forward
top level: BBa_J23018
start: 1940
end: 1959
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975740
display id: annotation1975740
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975740/range1975740/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975740/range1975740/1
type: Range
persistent identity: range1975740
display id: range1975740
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2292
end: 2297
orientation: inline

BBa_R0040 (Ptet)
type: SequenceAnnotation
persistent identity: annotation1893436
display id: annotation1893436
version: 1
top level: BBa_J23018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1893436/range1893436/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1893436/range1893436/1
type: Range
persistent identity: range1893436
display id: range1893436
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2217
end: 2270
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975739
display id: annotation1975739
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975739/range1975739/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975739/range1975739/1
type: Range
persistent identity: range1975739
display id: range1975739
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2064
end: 2071
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1975763
display id: annotation1975763
version: 1
top level: BBa_J23018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975763/range1975763/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975763/range1975763/1
type: Range
persistent identity: range1975763
display id: range1975763
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2279
end: 2290
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975736
display id: annotation1975736
version: 1
top level: BBa_J23018
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975736/range1975736/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975736/range1975736/1
type: Range
persistent identity: range1975736
display id: range1975736
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2091
end: 2134
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975764
display id: annotation1975764
version: 1
top level: BBa_J23018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975764/range1975764/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975764/range1975764/1
type: Range
persistent identity: range1975764
display id: range1975764
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2279
end: 2290
orientation: inline

BBa_B0015 terminator
type: SequenceAnnotation
persistent identity: annotation1893435
display id: annotation1893435
version: 1
top level: BBa_J23018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1893435/range1893435/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1893435/range1893435/1
type: Range
persistent identity: range1893435
display id: range1893435
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2080
end: 2208
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1893437
display id: annotation1893437
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1893437/range1893437/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1893437/range1893437/1
type: Range
persistent identity: range1893437
display id: range1893437
version: 1
direction: direction/reverse
top level: BBa_J23018
start: 183
end: 802
orientation: inline

pMB1 origin
type: SequenceAnnotation
persistent identity: annotation1934517
display id: annotation1934517
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1934517/range1934517/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1934517/range1934517/1
type: Range
persistent identity: range1934517
display id: range1934517
version: 1
direction: direction/reverse
top level: BBa_J23018
start: 180
end: 895
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1975735
display id: annotation1975735
version: 1
top level: BBa_J23018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975735/range1975735/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975735/range1975735/1
type: Range
persistent identity: range1975735
display id: range1975735
version: 1
direction: direction/reverse
top level: BBa_J23018
start: 1859
end: 1887
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975761
display id: annotation1975761
version: 1
top level: BBa_J23018
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975761/range1975761/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975761/range1975761/1
type: Range
persistent identity: range1975761
display id: range1975761
version: 1
direction: direction/reverse
top level: BBa_J23018
start: 79
end: 98
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975737
display id: annotation1975737
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975737/range1975737/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975737/range1975737/1
type: Range
persistent identity: range1975737
display id: range1975737
version: 1
direction: direction/forward
top level: BBa_J23018
start: 1
end: 21
orientation: inline

Ptet
type: SequenceAnnotation
persistent identity: annotation1975765
display id: annotation1975765
version: 1
top level: BBa_J23018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975765/range1975765/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975765/range1975765/1
type: Range
persistent identity: range1975765
display id: range1975765
version: 1
direction: direction/forward
top level: BBa_J23018
start: 2217
end: 2270
orientation: inline

BBa_J23019
type: ComponentDefinition
persistent identity: BBa J23019
display id: BBa_J23019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"
creator: John Anderson
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Variant of pBca1021 (J61003) with GFP inserted. Part J23019 is the same as J23018 but in pSB3C6.
mutable notes: N/A
mutable provenance: J23018 pAC997
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rrnB T1 terminator
sequence annotation: BBa_B0034 RBS
sequence annotation: RBS
sequence annotation: NotI
sequence annotation: BioBrick suffix
sequence annotation: BBa_R0040 (Ptet)
sequence annotation: chloramphenicol acetyl transferase (CmR resistance marker)
sequence annotation: p15A origin
sequence annotation: XbaI
sequence annotation: BBa_B0015 terminator
sequence annotation: Ptet
sequence annotation: EcoRI
sequence: https://synbiohub.org/public/igem/BBa_J23019_sequence/1

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975743
display id: annotation1975743
version: 1
top level: BBa_J23019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975743/range1975743/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975743/range1975743/1
type: Range
persistent identity: range1975743
display id: range1975743
version: 1
direction: direction/forward
top level: BBa_J23019
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BBa_B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1975759
display id: annotation1975759
version: 1
top level: BBa_J23019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975759/range1975759/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975759/range1975759/1
type: Range
persistent identity: range1975759
display id: range1975759
version: 1
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start: 2265
end: 2276
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975758
display id: annotation1975758
version: 1
top level: BBa_J23019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975758/range1975758/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975758/range1975758/1
type: Range
persistent identity: range1975758
display id: range1975758
version: 1
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start: 2265
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orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975745
display id: annotation1975745
version: 1
top level: BBa_J23019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975745/range1975745/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975745/range1975745/1
type: Range
persistent identity: range1975745
display id: range1975745
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2050
end: 2057
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1893440
display id: annotation1893440
version: 1
top level: BBa_J23019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1893440/range1893440/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1893440/range1893440/1
type: Range
persistent identity: range1893440
display id: range1893440
version: 1
direction: direction/forward
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start: 1
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BBa_R0040 (Ptet)
type: SequenceAnnotation
persistent identity: annotation1893439
display id: annotation1893439
version: 1
top level: BBa_J23019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1893439/range1893439/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1893439/range1893439/1
type: Range
persistent identity: range1893439
display id: range1893439
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2203
end: 2256
orientation: inline

chloramphenicol acetyl transferase (CmR resistance marker)
type: SequenceAnnotation
persistent identity: annotation1975742
display id: annotation1975742
version: 1
top level: BBa_J23019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975742/range1975742/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975742/range1975742/1
type: Range
persistent identity: range1975742
display id: range1975742
version: 1
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top level: BBa_J23019
start: 1232
end: 1891
orientation: inline

p15A origin
type: SequenceAnnotation
persistent identity: annotation1975741
display id: annotation1975741
version: 1
top level: BBa_J23019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975741/range1975741/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975741/range1975741/1
type: Range
persistent identity: range1975741
display id: range1975741
version: 1
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start: 22
end: 870
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975746
display id: annotation1975746
version: 1
top level: BBa_J23019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975746/range1975746/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975746/range1975746/1
type: Range
persistent identity: range1975746
display id: range1975746
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2278
end: 2283
orientation: inline

BBa_B0015 terminator
type: SequenceAnnotation
persistent identity: annotation1893438
display id: annotation1893438
version: 1
top level: BBa_J23019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1893438/range1893438/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1893438/range1893438/1
type: Range
persistent identity: range1893438
display id: range1893438
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2066
end: 2194
orientation: inline

Ptet
type: SequenceAnnotation
persistent identity: annotation1975762
display id: annotation1975762
version: 1
top level: BBa_J23019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975762/range1975762/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975762/range1975762/1
type: Range
persistent identity: range1975762
display id: range1975762
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2203
end: 2256
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975744
display id: annotation1975744
version: 1
top level: BBa_J23019
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975744/range1975744/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975744/range1975744/1
type: Range
persistent identity: range1975744
display id: range1975744
version: 1
direction: direction/forward
top level: BBa_J23019
start: 2044
end: 2049
orientation: inline