//plasmidbackbone/libraryscreening/promoter
type: Collection
persistent identity: plasmidbackbone libraryscreening promoter
display id: plasmidbackbone_libraryscreening_promoter
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: pSBBs1C
member: p68
member: BBa_K777133
member: BBa_J61002
member: pSBBs3C

p68
type: ComponentDefinition
persistent identity: BBa K678070
display id: BBa_K678070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p68
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: argB marker cassette
sequence annotation: TtrpC
sequence annotation: ampR
sequence annotation: IS-down
sequence annotation: lacZ
sequence annotation: USER cassette (AsiSI/Nb.BtsI)
sequence annotation: IS-up
sequence: https://synbiohub.org/public/igem/BBa_K678070_sequence/1

argB marker cassette
type: SequenceAnnotation
persistent identity: annotation2135110
display id: annotation2135110
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135110/range2135110/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135110/range2135110/1
type: Range
persistent identity: range2135110
display id: range2135110
version: 1
direction: direction/forward
top level: p68
start: 7171
end: 9327
orientation: inline

TtrpC
type: SequenceAnnotation
persistent identity: annotation2135109
display id: annotation2135109
version: 1
top level: p68
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135109/range2135109/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135109/range2135109/1
type: Range
persistent identity: range2135109
display id: range2135109
version: 1
direction: direction/forward
top level: p68
start: 6396
end: 7157
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2135105
display id: annotation2135105
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135105/range2135105/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135105/range2135105/1
type: Range
persistent identity: range2135105
display id: range2135105
version: 1
direction: direction/forward
top level: p68
start: 1
end: 859
orientation: inline

IS-down
type: SequenceAnnotation
persistent identity: annotation2135111
display id: annotation2135111
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135111/range2135111/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135111/range2135111/1
type: Range
persistent identity: range2135111
display id: range2135111
version: 1
direction: direction/forward
top level: p68
start: 9342
end: 11267
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation2135108
display id: annotation2135108
version: 1
top level: p68
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135108/range2135108/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135108/range2135108/1
type: Range
persistent identity: range2135108
display id: range2135108
version: 1
direction: direction/forward
top level: p68
start: 3319
end: 6392
orientation: inline

USER cassette (AsiSI/Nb.BtsI)
type: SequenceAnnotation
persistent identity: annotation2135107
display id: annotation2135107
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135107/range2135107/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135107/range2135107/1
type: Range
persistent identity: range2135107
display id: range2135107
version: 1
direction: direction/forward
top level: p68
start: 3285
end: 3318
orientation: inline

IS-up
type: SequenceAnnotation
persistent identity: annotation2135106
display id: annotation2135106
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135106/range2135106/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135106/range2135106/1
type: Range
persistent identity: range2135106
display id: range2135106
version: 1
direction: direction/forward
top level: p68
start: 1318
end: 3266
orientation: inline

BBa_K777133
type: ComponentDefinition
persistent identity: BBa K777133
display id: BBa_K777133
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Similar to pSB1C3, with RFP reporter
creator: Team Goettingen
created: 2012-09-27T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to [http://partsregistry.org/Part:BBa_J61002 J61002] but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.
mutable notes: * No specific design considerations.
mutable provenance: * This part is a compination of E1010, B0034 and pSB1C3 from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rep (pMB1)
sequence annotation: XbaI
sequence annotation: PstI
sequence annotation: VF2 primer binding site
sequence annotation: EcoRI
sequence annotation: SpeI
sequence annotation: mRFP1
sequence annotation: Chloramphenicol resistance
sequence annotation: BB0034
sequence annotation: RBS
sequence annotation: E1010
sequence annotation: VR primer binding site
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_K777133_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2203655
display id: annotation2203655
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203655/range2203655/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203655/range2203655/1
type: Range
persistent identity: range2203655
display id: range2203655
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1129
end: 1735
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2203668
display id: annotation2203668
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203668/range2203668/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203668/range2203668/1
type: Range
persistent identity: range2203668
display id: range2203668
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2932
end: 2937
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2203666
display id: annotation2203666
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203666/range2203666/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203666/range2203666/1
type: Range
persistent identity: range2203666
display id: range2203666
version: 1
direction: direction/forward
top level: BBa_K777133
start: 884
end: 889
orientation: inline

VF2 primer binding site
type: SequenceAnnotation
persistent identity: annotation2203664
display id: annotation2203664
version: 1
top level: BBa_K777133
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203664/range2203664/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203664/range2203664/1
type: Range
persistent identity: range2203664
display id: range2203664
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2799
end: 2818
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2203667
display id: annotation2203667
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203667/range2203667/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203667/range2203667/1
type: Range
persistent identity: range2203667
display id: range2203667
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2917
end: 2922
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2203665
display id: annotation2203665
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203665/range2203665/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203665/range2203665/1
type: Range
persistent identity: range2203665
display id: range2203665
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1
end: 6
orientation: inline

mRFP1
type: SequenceAnnotation
persistent identity: annotation2203653
display id: annotation2203653
version: 1
top level: BBa_K777133
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203653/range2203653/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203653/range2203653/1
type: Range
persistent identity: range2203653
display id: range2203653
version: 1
direction: direction/forward
top level: BBa_K777133
start: 26
end: 703
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2203657
display id: annotation2203657
version: 1
top level: BBa_K777133
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203657/range2203657/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203657/range2203657/1
type: Range
persistent identity: range2203657
display id: range2203657
version: 1
direction: direction/reverse
top level: BBa_K777133
start: 2028
end: 2687
orientation: inline

BB0034
type: SequenceAnnotation
persistent identity: annotation2203652
display id: annotation2203652
version: 1
top level: BBa_K777133
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203652/range2203652/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203652/range2203652/1
type: Range
persistent identity: range2203652
display id: range2203652
version: 1
direction: direction/forward
top level: BBa_K777133
start: 8
end: 19
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203651
display id: annotation2203651
version: 1
top level: BBa_K777133
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203651/range2203651/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203651/range2203651/1
type: Range
persistent identity: range2203651
display id: range2203651
version: 1
direction: direction/forward
top level: BBa_K777133
start: 8
end: 19
orientation: inline

E1010
type: SequenceAnnotation
persistent identity: annotation2203654
display id: annotation2203654
version: 1
top level: BBa_K777133
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203654/range2203654/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203654/range2203654/1
type: Range
persistent identity: range2203654
display id: range2203654
version: 1
direction: direction/forward
top level: BBa_K777133
start: 26
end: 703
orientation: inline

VR primer binding site
type: SequenceAnnotation
persistent identity: annotation2203663
display id: annotation2203663
version: 1
top level: BBa_K777133
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203663/range2203663/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203663/range2203663/1
type: Range
persistent identity: range2203663
display id: range2203663
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1023
end: 1042
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2203662
display id: annotation2203662
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203662/range2203662/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203662/range2203662/1
type: Range
persistent identity: range2203662
display id: range2203662
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1144
end: 1144
orientation: inline

BBa_J61002
type: ComponentDefinition
persistent identity: BBa J61002
display id: BBa_J61002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EX-Ptet-S-rbsRFP-P "RFP reporter"
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid for construction of promoter basic parts. Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.
mutable notes: N/A
mutable provenance: PCR ca1020F/R on pSB1A2-I13521 (900bp, SpeI/PstI) Sub into pSB1A2-I13522 (SpeI/PstI) Product is pBca1020, will be an iGEM number ultimately ---- ca1020F Forward SpeI oligo for X-rbsRFP-P plasmid gcactACTAGTgaaagaggagaaatactagatggc ca1020R Reverse PstI oligo for X-rbsRFP-P plasmid ccggactgcagcggccgcttctagtatataaacg
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: Ambiguity; should be a T
sequence annotation: BBa_B0034 RBS
sequence annotation: SpeI
sequence annotation: mRFP
sequence annotation: NotI
sequence annotation: PstI
sequence annotation: BBa_B0015
sequence annotation: BioBrick prefix
sequence annotation: ColE1 origin
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: RBS
sequence annotation: rrnB terminator
sequence annotation: beta-lactamase (ampR, bla) promoter
sequence annotation: pBR322 origin
sequence: https://synbiohub.org/public/igem/BBa_J61002_sequence/1

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975769
display id: annotation1975769
version: 1
top level: BBa_J61002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975769/range1975769/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975769/range1975769/1
type: Range
persistent identity: range1975769
display id: range1975769
version: 1
direction: direction/forward
top level: BBa_J61002
start: 2809
end: 2828
orientation: inline

Ambiguity; should be a T
type: SequenceAnnotation
persistent identity: annotation1975921
display id: annotation1975921
version: 1
top level: BBa_J61002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975921/range1975921/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975921/range1975921/1
type: Range
persistent identity: range1975921
display id: range1975921
version: 1
direction: direction/forward
top level: BBa_J61002
start: 2941
end: 2941
orientation: inline

BBa_B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1975767
display id: annotation1975767
version: 1
top level: BBa_J61002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975767/range1975767/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975767/range1975767/1
type: Range
persistent identity: range1975767
display id: range1975767
version: 1
direction: direction/forward
top level: BBa_J61002
start: 9
end: 20
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1975716
display id: annotation1975716
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975716/range1975716/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975716/range1975716/1
type: Range
persistent identity: range1975716
display id: range1975716
version: 1
direction: direction/forward
top level: BBa_J61002
start: 2
end: 7
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation1892273
display id: annotation1892273
version: 1
top level: BBa_J61002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892273/range1892273/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892273/range1892273/1
type: Range
persistent identity: range1892273
display id: range1892273
version: 1
direction: direction/forward
top level: BBa_J61002
start: 27
end: 707
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975718
display id: annotation1975718
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975718/range1975718/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975718/range1975718/1
type: Range
persistent identity: range1975718
display id: range1975718
version: 1
direction: direction/forward
top level: BBa_J61002
start: 878
end: 885
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1975717
display id: annotation1975717
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975717/range1975717/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975717/range1975717/1
type: Range
persistent identity: range1975717
display id: range1975717
version: 1
direction: direction/forward
top level: BBa_J61002
start: 885
end: 890
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation1892274
display id: annotation1892274
version: 1
top level: BBa_J61002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892274/range1892274/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892274/range1892274/1
type: Range
persistent identity: range1892274
display id: range1892274
version: 1
direction: direction/forward
top level: BBa_J61002
start: 741
end: 869
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1975715
display id: annotation1975715
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975715/range1975715/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975715/range1975715/1
type: Range
persistent identity: range1975715
display id: range1975715
version: 1
direction: direction/forward
top level: BBa_J61002
start: 2927
end: 2948
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1892275
display id: annotation1892275
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892275/range1892275/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892275/range1892275/1
type: Range
persistent identity: range1892275
display id: range1892275
version: 1
direction: direction/reverse
top level: BBa_J61002
start: 1049
end: 1731
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975768
display id: annotation1975768
version: 1
top level: BBa_J61002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975768/range1975768/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975768/range1975768/1
type: Range
persistent identity: range1975768
display id: range1975768
version: 1
direction: direction/reverse
top level: BBa_J61002
start: 948
end: 967
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1892276
display id: annotation1892276
version: 1
top level: BBa_J61002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892276/range1892276/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892276/range1892276/1
type: Range
persistent identity: range1892276
display id: range1892276
version: 1
direction: direction/reverse
top level: BBa_J61002
start: 1826
end: 2686
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975766
display id: annotation1975766
version: 1
top level: BBa_J61002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975766/range1975766/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975766/range1975766/1
type: Range
persistent identity: range1975766
display id: range1975766
version: 1
direction: direction/forward
top level: BBa_J61002
start: 9
end: 20
orientation: inline

rrnB terminator
type: SequenceAnnotation
persistent identity: annotation1975713
display id: annotation1975713
version: 1
top level: BBa_J61002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975713/range1975713/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975713/range1975713/1
type: Range
persistent identity: range1975713
display id: range1975713
version: 1
direction: direction/forward
top level: BBa_J61002
start: 752
end: 795
orientation: inline

beta-lactamase (ampR, bla) promoter
type: SequenceAnnotation
persistent identity: annotation1892272
display id: annotation1892272
version: 1
top level: BBa_J61002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892272/range1892272/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892272/range1892272/1
type: Range
persistent identity: range1892272
display id: range1892272
version: 1
direction: direction/reverse
top level: BBa_J61002
start: 2728
end: 2756
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975714
display id: annotation1975714
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975714/range1975714/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975714/range1975714/1
type: Range
persistent identity: range1975714
display id: range1975714
version: 1
direction: direction/reverse
top level: BBa_J61002
start: 1052
end: 1671
orientation: inline

pSBBs3C
type: ComponentDefinition
persistent identity: BBa K823025
display id: BBa_K823025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs3C-luxABCDE (lux reporter vector for B.subtilis)
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a reporter vector for Bacillus subtilis. It integrates at the sacA locus of B.subtilis genome. It has a ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for selection in B. subtilis. The multiple cloning site has a rfp for selection and is followed by the luxABCDE gene cluster for luminescence reporter assays. This BioBrick is part of the LMU-Munich 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
mutable provenance: This vector is an igem compatible version of the pAH328 Bacillus reporter vecotr.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: luxA
sequence annotation: luxC
sequence annotation: luxB
sequence annotation: sacA
sequence annotation: luxE
sequence annotation: cat
sequence annotation: luxD
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_K823025_sequence/1

luxA
type: SequenceAnnotation
persistent identity: annotation2180247
display id: annotation2180247
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180247/range2180247/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180247/range2180247/1
type: Range
persistent identity: range2180247
display id: range2180247
version: 1
direction: direction/forward
top level: pSBBs3C
start: 73
end: 1155
orientation: inline

luxC
type: SequenceAnnotation
persistent identity: annotation2180249
display id: annotation2180249
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180249/range2180249/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180249/range2180249/1
type: Range
persistent identity: range2180249
display id: range2180249
version: 1
direction: direction/forward
top level: pSBBs3C
start: 2179
end: 3621
orientation: inline

luxB
type: SequenceAnnotation
persistent identity: annotation2180248
display id: annotation2180248
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180248/range2180248/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180248/range2180248/1
type: Range
persistent identity: range2180248
display id: range2180248
version: 1
direction: direction/forward
top level: pSBBs3C
start: 1172
end: 2155
orientation: inline

sacA
type: SequenceAnnotation
persistent identity: annotation2180252
display id: annotation2180252
version: 1
top level: pSBBs3C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180252/range2180252/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180252/range2180252/1
type: Range
persistent identity: range2180252
display id: range2180252
version: 1
direction: direction/forward
top level: pSBBs3C
start: 5676
end: 6515
orientation: inline

luxE
type: SequenceAnnotation
persistent identity: annotation2180251
display id: annotation2180251
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180251/range2180251/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180251/range2180251/1
type: Range
persistent identity: range2180251
display id: range2180251
version: 1
direction: direction/forward
top level: pSBBs3C
start: 4583
end: 5695
orientation: inline

sacA
type: SequenceAnnotation
persistent identity: annotation2180254
display id: annotation2180254
version: 1
top level: pSBBs3C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180254/range2180254/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180254/range2180254/1
type: Range
persistent identity: range2180254
display id: range2180254
version: 1
direction: direction/forward
top level: pSBBs3C
start: 8712
end: 9503
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2180255
display id: annotation2180255
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180255/range2180255/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180255/range2180255/1
type: Range
persistent identity: range2180255
display id: range2180255
version: 1
direction: direction/reverse
top level: pSBBs3C
start: 9661
end: 10323
orientation: inline

luxD
type: SequenceAnnotation
persistent identity: annotation2180250
display id: annotation2180250
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180250/range2180250/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180250/range2180250/1
type: Range
persistent identity: range2180250
display id: range2180250
version: 1
direction: direction/forward
top level: pSBBs3C
start: 3635
end: 4558
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180253
display id: annotation2180253
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180253/range2180253/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180253/range2180253/1
type: Range
persistent identity: range2180253
display id: range2180253
version: 1
direction: direction/forward
top level: pSBBs3C
start: 6849
end: 7721
orientation: inline

pSBBs1C
type: ComponentDefinition
persistent identity: BBa K823021
display id: BBa_K823021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs1C-lacZ (lacZ reporter vector for B. subtilis)
creator: Jara Radeck
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a backbone vector for Bacillus subtilis. It integrates at the amyE locus. It has a Ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: This reporter vector can be used with the normal BioBrick assembly strategies.
mutable provenance: This vector is the Bacillus vector pAC6 brought into the iGEM BioBrick standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lacZ
sequence annotation: cat
sequence annotation: 'amyE
sequence annotation: amyE'
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_K823021_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation2179602
display id: annotation2179602
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179602/range2179602/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179602/range2179602/1
type: Range
persistent identity: range2179602
display id: range2179602
version: 1
direction: direction/forward
top level: pSBBs1C
start: 49
end: 3112
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2179606
display id: annotation2179606
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179606/range2179606/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179606/range2179606/1
type: Range
persistent identity: range2179606
display id: range2179606
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 8813
end: 9463
orientation: inline

'amyE
type: SequenceAnnotation
persistent identity: annotation2179605
display id: annotation2179605
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179605/range2179605/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179605/range2179605/1
type: Range
persistent identity: range2179605
display id: range2179605
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 7402
end: 8229
orientation: inline

amyE'
type: SequenceAnnotation
persistent identity: annotation2179603
display id: annotation2179603
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179603/range2179603/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179603/range2179603/1
type: Range
persistent identity: range2179603
display id: range2179603
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 3165
end: 3698
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2179604
display id: annotation2179604
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179604/range2179604/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179604/range2179604/1
type: Range
persistent identity: range2179604
display id: range2179604
version: 1
direction: direction/forward
top level: pSBBs1C
start: 4023
end: 4883
orientation: inline