This part is similar to [http://partsregistry.org/Part:BBa_J61002 J61002] but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.
* This part is a compination of E1010, B0034 and pSB1C3 from the registry.
Plasmid for construction of promoter basic parts. Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.
PCR ca1020F/R on pSB1A2-I13521 (900bp, SpeI/PstI) Sub into pSB1A2-I13522 (SpeI/PstI) Product is pBca1020, will be an iGEM number ultimately ---- ca1020F Forward SpeI oligo for X-rbsRFP-P plasmid gcactACTAGTgaaagaggagaaatactagatggc ca1020R Reverse PstI oligo for X-rbsRFP-P plasmid ccggactgcagcggccgcttctagtatataaacg
Verification forward (VF2) primer binding site
Verification reverse (VR) primer binding site
beta-lactamase ampicillin resistance marker
pSBBs3C-luxABCDE (lux reporter vector for B.subtilis)
This part is a reporter vector for Bacillus subtilis. It integrates at the sacA locus of B.subtilis genome. It has a ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for selection in B. subtilis. The multiple cloning site has a rfp for selection and is followed by the luxABCDE gene cluster for luminescence reporter assays. This BioBrick is part of the LMU-Munich 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
This vector is an igem compatible version of the pAH328 Bacillus reporter vecotr.
pSBBs1C-lacZ (lacZ reporter vector for B. subtilis)
This part is a backbone vector for Bacillus subtilis. It integrates at the amyE locus. It has a Ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
This reporter vector can be used with the normal BioBrick assembly strategies.
This vector is the Bacillus vector pAC6 brought into the iGEM BioBrick standard.