//plasmidbackbone/synthesis
type: Collection
persistent identity: plasmidbackbone synthesis
display id: plasmidbackbone_synthesis
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: pUC57
member: GeneArt pG
member: GENEART pG
member: pSB1A1

GeneArt pG
type: ComponentDefinition
persistent identity: BBa J70003
display id: BBa_J70003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Geneart Cloning Plasmid pGA4
creator: Randy Rettberg
created: 2008-01-16T12:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_1_
m user id: 0
owner id: 25
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cloning plasmid used by Geneart for iGEM parts. This plasmid does not have a standard BioBrick cloning site.
mutable notes: This plasmid needs to be checked to se where arets have actually been inserted. - Randy
mutable provenance: Geneart
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: D_Segment
sequence annotation: T7 Promoter
sequence annotation: AmpR
sequence annotation: ColE1 Origin
sequence annotation: T3 Promoter
sequence annotation: f1 (+) Origin
sequence: https://synbiohub.org/public/igem/BBa_J70003_sequence/1

D_Segment
type: SequenceAnnotation
persistent identity: annotation1959608
display id: annotation1959608
version: 1
top level: GeneArt pG
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70003/annotation1959608/range1959608/1

https://synbiohub.org/public/igem/BBa_J70003/annotation1959608/range1959608/1
type: Range
persistent identity: range1959608
display id: range1959608
version: 1
direction: direction/reverse
top level: GeneArt pG
start: 500
end: 658
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation1959605
display id: annotation1959605
version: 1
top level: GeneArt pG
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70003/annotation1959605/range1959605/1

https://synbiohub.org/public/igem/BBa_J70003/annotation1959605/range1959605/1
type: Range
persistent identity: range1959605
display id: range1959605
version: 1
direction: direction/forward
top level: GeneArt pG
start: 624
end: 646
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation1959607
display id: annotation1959607
version: 1
top level: GeneArt pG
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70003/annotation1959607/range1959607/1

https://synbiohub.org/public/igem/BBa_J70003/annotation1959607/range1959607/1
type: Range
persistent identity: range1959607
display id: range1959607
version: 1
direction: direction/reverse
top level: GeneArt pG
start: 1975
end: 2832
orientation: inline

ColE1 Origin
type: SequenceAnnotation
persistent identity: annotation1959610
display id: annotation1959610
version: 1
top level: GeneArt pG
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70003/annotation1959610/range1959610/1

https://synbiohub.org/public/igem/BBa_J70003/annotation1959610/range1959610/1
type: Range
persistent identity: range1959610
display id: range1959610
version: 1
direction: direction/reverse
top level: GeneArt pG
start: 1232
end: 1672
orientation: inline

T3 Promoter
type: SequenceAnnotation
persistent identity: annotation1959606
display id: annotation1959606
version: 1
top level: GeneArt pG
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70003/annotation1959606/range1959606/1

https://synbiohub.org/public/igem/BBa_J70003/annotation1959606/range1959606/1
type: Range
persistent identity: range1959606
display id: range1959606
version: 1
direction: direction/reverse
top level: GeneArt pG
start: 772
end: 789
orientation: inline

f1 (+) Origin
type: SequenceAnnotation
persistent identity: annotation1959609
display id: annotation1959609
version: 1
top level: GeneArt pG
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70003/annotation1959609/range1959609/1

https://synbiohub.org/public/igem/BBa_J70003/annotation1959609/range1959609/1
type: Range
persistent identity: range1959609
display id: range1959609
version: 1
direction: direction/reverse
top level: GeneArt pG
start: 3
end: 459
orientation: inline

pSB1A1
type: ComponentDefinition
persistent identity: pSB1A1
display id: pSB1A1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pUC19 with a BioBrick cloning site (Replaced by pSB1A3)
creator: Reshma Shetty
created: 2006-10-23T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This plasmid is pUC19 with a BioBricks cloning site (EcoRI-NotI-XbaI-SpeI-NotI-PstI) located between the EcoRI and BamHI sites in the multiple cloning site of pUC19.
mutable notes: It is not entirely clear whether there are two contigous copies of EcoRI restriction site or one copy of the EcoRI in this vector. Sequence information did not include this section of the vector. In this part, only a single EcoRI restriction site is shown.
mutable provenance: pUC19 is an extremely common cloning vector. This particular version is entered in the registry because several parts synthesized by Codon Devices (BBa_P1002, BBa_P1003, BBa_P1004, BBa_P1005, BBa_I50040) were returned in this vector. Sequence information can be found at http://www.fermentas.com/techinfo/nucleicacids/sequences/puc19.txt
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RNAI transcript promoter -35 site
sequence annotation: bla RNA transcription start
sequence annotation: beta-lactamase signal peptide
sequence annotation: M13 Forward priming site
sequence annotation: beta-lactamase (bla; amp-r)
sequence annotation: P(LAC) -10 site
sequence annotation: P(BLA) -10 site
sequence annotation: RNAII transcript
sequence annotation: M13Reverse priming site
sequence annotation: CAP protein binding site
sequence annotation: BioBricks suffix
sequence annotation: RNAII transcript promoter -35 site
sequence annotation: RNAI transcript
sequence annotation: Lac operon
sequence annotation: pBR322
sequence annotation: P(LAC) transcription start site
sequence annotation: ORI
sequence annotation: P(LAC) -35 site
sequence annotation: P(BLA) -35 site
sequence annotation: origin of replication
sequence annotation: BioBricks prefix
sequence annotation: RNAII transcript promoter -10 site
sequence: https://synbiohub.org/public/igem/pSB1A1_sequence/1

RNAI transcript promoter -35 site
type: SequenceAnnotation
persistent identity: annotation1904909
display id: annotation1904909
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904909/range1904909/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904909/range1904909/1
type: Range
persistent identity: range1904909
display id: range1904909
version: 1
direction: direction/forward
top level: pSB1A1
start: 900
end: 906
orientation: inline

bla RNA transcription start
type: SequenceAnnotation
persistent identity: annotation1904915
display id: annotation1904915
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904915/range1904915/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904915/range1904915/1
type: Range
persistent identity: range1904915
display id: range1904915
version: 1
direction: direction/reverse
top level: pSB1A1
start: 2126
end: 2126
orientation: inline

beta-lactamase signal peptide
type: SequenceAnnotation
persistent identity: annotation1904914
display id: annotation1904914
version: 1
top level: pSB1A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904914/range1904914/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904914/range1904914/1
type: Range
persistent identity: range1904914
display id: range1904914
version: 1
direction: direction/reverse
top level: pSB1A1
start: 2023
end: 2091
orientation: inline

M13 Forward priming site
type: SequenceAnnotation
persistent identity: annotation1904919
display id: annotation1904919
version: 1
top level: pSB1A1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904919/range1904919/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904919/range1904919/1
type: Range
persistent identity: range1904919
display id: range1904919
version: 1
direction: direction/forward
top level: pSB1A1
start: 2670
end: 2685
orientation: inline

beta-lactamase (bla; amp-r)
type: SequenceAnnotation
persistent identity: annotation1904913
display id: annotation1904913
version: 1
top level: pSB1A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904913/range1904913/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904913/range1904913/1
type: Range
persistent identity: range1904913
display id: range1904913
version: 1
direction: direction/reverse
top level: pSB1A1
start: 1231
end: 2091
orientation: inline

P(LAC) -10 site
type: SequenceAnnotation
persistent identity: annotation1904900
display id: annotation1904900
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904900/range1904900/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904900/range1904900/1
type: Range
persistent identity: range1904900
display id: range1904900
version: 1
direction: direction/reverse
top level: pSB1A1
start: 119
end: 124
orientation: inline

P(BLA) -10 site
type: SequenceAnnotation
persistent identity: annotation1904916
display id: annotation1904916
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904916/range1904916/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904916/range1904916/1
type: Range
persistent identity: range1904916
display id: range1904916
version: 1
direction: direction/reverse
top level: pSB1A1
start: 2135
end: 2140
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1904905
display id: annotation1904905
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904905/range1904905/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904905/range1904905/1
type: Range
persistent identity: range1904905
display id: range1904905
version: 1
direction: direction/reverse
top level: pSB1A1
start: 472
end: 1024
orientation: inline

M13Reverse priming site
type: SequenceAnnotation
persistent identity: annotation1904898
display id: annotation1904898
version: 1
top level: pSB1A1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904898/range1904898/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904898/range1904898/1
type: Range
persistent identity: range1904898
display id: range1904898
version: 1
direction: direction/reverse
top level: pSB1A1
start: 70
end: 86
orientation: inline

CAP protein binding site
type: SequenceAnnotation
persistent identity: annotation1904902
display id: annotation1904902
version: 1
top level: pSB1A1
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904902/range1904902/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904902/range1904902/1
type: Range
persistent identity: range1904902
display id: range1904902
version: 1
direction: direction/reverse
top level: pSB1A1
start: 168
end: 180
orientation: inline

BioBricks suffix
type: SequenceAnnotation
persistent identity: annotation1904920
display id: annotation1904920
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904920/range1904920/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904920/range1904920/1
type: Range
persistent identity: range1904920
display id: range1904920
version: 1
direction: direction/forward
top level: pSB1A1
start: 1
end: 21
orientation: inline

RNAII transcript promoter -35 site
type: SequenceAnnotation
persistent identity: annotation1904911
display id: annotation1904911
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904911/range1904911/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904911/range1904911/1
type: Range
persistent identity: range1904911
display id: range1904911
version: 1
direction: direction/reverse
top level: pSB1A1
start: 1055
end: 1060
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1904907
display id: annotation1904907
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904907/range1904907/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904907/range1904907/1
type: Range
persistent identity: range1904907
display id: range1904907
version: 1
direction: direction/forward
top level: pSB1A1
start: 914
end: 1021
orientation: inline

Lac operon
type: SequenceAnnotation
persistent identity: annotation1904903
display id: annotation1904903
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904903/range1904903/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904903/range1904903/1
type: Range
persistent identity: range1904903
display id: range1904903
version: 1
direction: direction/reverse
top level: pSB1A1
start: 60
end: 289
orientation: inline

pBR322
type: SequenceAnnotation
persistent identity: annotation1904918
display id: annotation1904918
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904918/range1904918/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904918/range1904918/1
type: Range
persistent identity: range1904918
display id: range1904918
version: 1
direction: direction/forward
top level: pSB1A1
start: 2292
end: 2428
orientation: inline

P(LAC) transcription start site
type: SequenceAnnotation
persistent identity: annotation1904899
display id: annotation1904899
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904899/range1904899/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904899/range1904899/1
type: Range
persistent identity: range1904899
display id: range1904899
version: 1
direction: direction/reverse
top level: pSB1A1
start: 112
end: 112
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1904904
display id: annotation1904904
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904904/range1904904/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904904/range1904904/1
type: Range
persistent identity: range1904904
display id: range1904904
version: 1
direction: direction/forward
top level: pSB1A1
start: 472
end: 472
orientation: inline

P(LAC) -35 site
type: SequenceAnnotation
persistent identity: annotation1904901
display id: annotation1904901
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904901/range1904901/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904901/range1904901/1
type: Range
persistent identity: range1904901
display id: range1904901
version: 1
direction: direction/reverse
top level: pSB1A1
start: 143
end: 148
orientation: inline

RNAI transcript promoter -35 site
type: SequenceAnnotation
persistent identity: annotation1904908
display id: annotation1904908
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904908/range1904908/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904908/range1904908/1
type: Range
persistent identity: range1904908
display id: range1904908
version: 1
direction: direction/forward
top level: pSB1A1
start: 878
end: 883
orientation: inline

pBR322
type: SequenceAnnotation
persistent identity: annotation1904912
display id: annotation1904912
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904912/range1904912/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904912/range1904912/1
type: Range
persistent identity: range1904912
display id: range1904912
version: 1
direction: direction/forward
top level: pSB1A1
start: 290
end: 2291
orientation: inline

P(BLA) -35 site
type: SequenceAnnotation
persistent identity: annotation1904917
display id: annotation1904917
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904917/range1904917/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904917/range1904917/1
type: Range
persistent identity: range1904917
display id: range1904917
version: 1
direction: direction/reverse
top level: pSB1A1
start: 2156
end: 2161
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1904906
display id: annotation1904906
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904906/range1904906/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904906/range1904906/1
type: Range
persistent identity: range1904906
display id: range1904906
version: 1
direction: direction/reverse
top level: pSB1A1
start: 472
end: 1060
orientation: inline

BioBricks prefix
type: SequenceAnnotation
persistent identity: annotation1904921
display id: annotation1904921
version: 1
top level: pSB1A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904921/range1904921/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904921/range1904921/1
type: Range
persistent identity: range1904921
display id: range1904921
version: 1
direction: direction/forward
top level: pSB1A1
start: 2687
end: 2708
orientation: inline

RNAII transcript promoter -10 site
type: SequenceAnnotation
persistent identity: annotation1904910
display id: annotation1904910
version: 1
top level: pSB1A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1A1/annotation1904910/range1904910/1

https://synbiohub.org/public/igem/pSB1A1/annotation1904910/range1904910/1
type: Range
persistent identity: range1904910
display id: range1904910
version: 1
direction: direction/reverse
top level: pSB1A1
start: 1034
end: 1039
orientation: inline

pUC57
type: ComponentDefinition
persistent identity: BBa J70004
display id: BBa_J70004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBasic Cloning Plasmid pUC57
creator: Meagan Lizarazo
created: 2008-01-23T12:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 457
owning group id: 165
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cloning plasmid used by Geneart for iGEM parts. This plasmid does not have a standard BioBrick cloning site. Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta). Vector is AmpR.
mutable notes: This plasmid needs to be checked to see where parts have actually been inserted. The sequence for this plasmid needs to be rotated so that the suffix and prefix are at the ends.
mutable provenance: Geneart
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: CAP
sequence annotation: AmpR
sequence annotation: pMB1 origin
sequence annotation: LacZ
sequence annotation: LacI (Lac repressor)
sequence: https://synbiohub.org/public/igem/BBa_J70004_sequence/1

CAP
type: SequenceAnnotation
persistent identity: annotation1960245
display id: annotation1960245
version: 1
top level: pUC57
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70004/annotation1960245/range1960245/1

https://synbiohub.org/public/igem/BBa_J70004/annotation1960245/range1960245/1
type: Range
persistent identity: range1960245
display id: range1960245
version: 1
direction: direction/reverse
top level: pUC57
start: 578
end: 615
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation1960242
display id: annotation1960242
version: 1
top level: pUC57
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70004/annotation1960242/range1960242/1

https://synbiohub.org/public/igem/BBa_J70004/annotation1960242/range1960242/1
type: Range
persistent identity: range1960242
display id: range1960242
version: 1
direction: direction/reverse
top level: pUC57
start: 1650
end: 2510
orientation: inline

pMB1 origin
type: SequenceAnnotation
persistent identity: annotation1960243
display id: annotation1960243
version: 1
top level: pUC57
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70004/annotation1960243/range1960243/1

https://synbiohub.org/public/igem/BBa_J70004/annotation1960243/range1960243/1
type: Range
persistent identity: range1960243
display id: range1960243
version: 1
direction: direction/forward
top level: pUC57
start: 876
end: 1490
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation1960241
display id: annotation1960241
version: 1
top level: pUC57
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70004/annotation1960241/range1960241/1

https://synbiohub.org/public/igem/BBa_J70004/annotation1960241/range1960241/1
type: Range
persistent identity: range1960241
display id: range1960241
version: 1
direction: direction/reverse
top level: pUC57
start: 146
end: 493
orientation: inline

LacI (Lac repressor)
type: SequenceAnnotation
persistent identity: annotation1960244
display id: annotation1960244
version: 1
top level: pUC57
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J70004/annotation1960244/range1960244/1

https://synbiohub.org/public/igem/BBa_J70004/annotation1960244/range1960244/1
type: Range
persistent identity: range1960244
display id: range1960244
version: 1
direction: direction/reverse
top level: pUC57
start: 511
end: 531
orientation: inline

GENEART pG
type: ComponentDefinition
persistent identity: BBa J70022
display id: BBa_J70022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GENEART pGA4 plasmid
creator: Reshma Shetty
created: 2008-09-24T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The sequence of the plasmid that contains BBa_I712016.
mutable notes: The BioBrick prefix is missing the 3' G and the BioBrick suffix is missing the 5' T.
mutable provenance: GENEART
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: pBR322 origin
sequence annotation: T3 promoter
sequence annotation: f1 origin
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: f1 (+) origin
sequence annotation: D segment
sequence annotation: T7 promoter
sequence annotation: lacZalpha reporter
sequence annotation: ColE1 origin
sequence annotation: BioBrick prefix
sequence annotation: BioBrick suffix
sequence annotation: lac promoter
sequence: https://synbiohub.org/public/igem/BBa_J70022_sequence/1

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1976835
display id: annotation1976835
version: 1
top level: GENEART pG
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976835/range1976835/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976835/range1976835/1
type: Range
persistent identity: range1976835
display id: range1976835
version: 1
direction: direction/reverse
top level: GENEART pG
start: 2210
end: 2238
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1976833
display id: annotation1976833
version: 1
top level: GENEART pG
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976833/range1976833/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976833/range1976833/1
type: Range
persistent identity: range1976833
display id: range1976833
version: 1
direction: direction/reverse
top level: GENEART pG
start: 534
end: 1153
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1976831
display id: annotation1976831
version: 1
top level: GENEART pG
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976831/range1976831/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976831/range1976831/1
type: Range
persistent identity: range1976831
display id: range1976831
version: 1
direction: direction/reverse
top level: GENEART pG
start: 107
end: 126
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1976836
display id: annotation1976836
version: 1
top level: GENEART pG
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976836/range1976836/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976836/range1976836/1
type: Range
persistent identity: range1976836
display id: range1976836
version: 1
direction: direction/reverse
top level: GENEART pG
start: 2431
end: 2737
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1976834
display id: annotation1976834
version: 1
top level: GENEART pG
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976834/range1976834/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976834/range1976834/1
type: Range
persistent identity: range1976834
display id: range1976834
version: 1
direction: direction/reverse
top level: GENEART pG
start: 1308
end: 2168
orientation: inline

f1 (+) origin
type: SequenceAnnotation
persistent identity: annotation1976840
display id: annotation1976840
version: 1
top level: GENEART pG
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976840/range1976840/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976840/range1976840/1
type: Range
persistent identity: range1976840
display id: range1976840
version: 1
direction: direction/reverse
top level: GENEART pG
start: 2299
end: 2755
orientation: inline

D segment
type: SequenceAnnotation
persistent identity: annotation1976841
display id: annotation1976841
version: 1
top level: GENEART pG
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976841/range1976841/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976841/range1976841/1
type: Range
persistent identity: range1976841
display id: range1976841
version: 1
direction: direction/reverse
top level: GENEART pG
start: 2796
end: 2954
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1976838
display id: annotation1976838
version: 1
top level: GENEART pG
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976838/range1976838/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976838/range1976838/1
type: Range
persistent identity: range1976838
display id: range1976838
version: 1
direction: direction/forward
top level: GENEART pG
start: 2922
end: 2940
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1976837
display id: annotation1976837
version: 1
top level: GENEART pG
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976837/range1976837/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976837/range1976837/1
type: Range
persistent identity: range1976837
display id: range1976837
version: 1
direction: direction/reverse
top level: GENEART pG
start: 2755
end: 2914
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1976839
display id: annotation1976839
version: 1
top level: GENEART pG
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976839/range1976839/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976839/range1976839/1
type: Range
persistent identity: range1976839
display id: range1976839
version: 1
direction: direction/reverse
top level: GENEART pG
start: 567
end: 1007
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1976830
display id: annotation1976830
version: 1
top level: GENEART pG
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976830/range1976830/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976830/range1976830/1
type: Range
persistent identity: range1976830
display id: range1976830
version: 1
direction: direction/forward
top level: GENEART pG
start: 2958
end: 2978
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1976829
display id: annotation1976829
version: 1
top level: GENEART pG
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976829/range1976829/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976829/range1976829/1
type: Range
persistent identity: range1976829
display id: range1976829
version: 1
direction: direction/forward
top level: GENEART pG
start: 1
end: 20
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1976832
display id: annotation1976832
version: 1
top level: GENEART pG
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70022/annotation1976832/range1976832/1

https://synbiohub.org/public/igem/BBa_J70022/annotation1976832/range1976832/1
type: Range
persistent identity: range1976832
display id: range1976832
version: 1
direction: direction/reverse
top level: GENEART pG
start: 196
end: 225
orientation: inline