//primer
type: Collection
persistent identity: primer
display id: primer
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //primer/reporter
member: //primer/plasmid
member: //primer/t3
member: //primer/part
member: //primer/t7
member: //primer/m13
member: //primer/sp6

//primer/plasmid
type: Collection
persistent identity: primer plasmid
display id: primer_plasmid
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //primer/plasmid/amplification

//primer/part/sequencing
type: Collection
persistent identity: primer part sequencing
display id: primer_part_sequencing
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_G00400
member: BBa_G00121
member: BBa_G00701
member: BBa_G00401
member: BBa_G00700
member: VF2
member: VR

//primer/t3
type: Collection
persistent identity: primer t3
display id: primer_t3
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: T3

//primer/reporter/cfp
type: Collection
persistent identity: primer reporter cfp
display id: primer_reporter_cfp
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: CFP/YFP-f

//primer/t7
type: Collection
persistent identity: primer t7
display id: primer_t7
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: CBDcex(T7)
member: BBa_K863113
member: CBDclos(T7
member: T7

//primer/reporter/gfp
type: Collection
persistent identity: primer reporter gfp
display id: primer_reporter_gfp
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: GFP-r
member: CBDcex(T7)
member: BBa_K863113
member: CBDclos(J6
member: CBDcex(J61

//primer/part/amplification
type: Collection
persistent identity: primer part amplification
display id: primer_part_amplification
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_G00511
member: BioBrick-r
member: BBa_G00121
member: BBa_G00401
member: BBa_G00500
member: BBa_G00530
member: VF2
member: BBa_G00520
member: BBa_G00400
member: BBa_G00531
member: BioBrick-f
member: BBa_G00501
member: BBa_G00600
member: BBa_G00120
member: BBa_G00601
member: BBa_G00700
member: BBa_G00521
member: VR
member: BBa_G00510

//primer/plasmid/amplification
type: Collection
persistent identity: primer plasmid amplification
display id: primer_plasmid_amplification
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: Suffix-F
member: Prefix-R

//primer/reporter/yfp
type: Collection
persistent identity: primer reporter yfp
display id: primer_reporter_yfp
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: CFP/YFP-f

//primer/sp6
type: Collection
persistent identity: primer sp6
display id: primer_sp6
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: SP6

//primer/reporter
type: Collection
persistent identity: primer reporter
display id: primer_reporter
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //primer/reporter/cfp
member: //primer/reporter/gfp
member: //primer/reporter/yfp

//primer/part
type: Collection
persistent identity: primer part
display id: primer_part
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //primer/part/sequencing
member: //primer/part/amplification

//primer/m13
type: Collection
persistent identity: primer m13
display id: primer_m13
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: M13-F(-20)
member: M13-R(-26)
member: M13-F(-40)
member: M13-F(-46)
member: M13-R(-46)

M13-F(-46)
type: ComponentDefinition
persistent identity: BBa G00106
display id: BBa_G00106
version: 1
was generated by: iGEM to SBOL conversion
description: M13-F (-46)
created: 2006-06-07T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: Ancient
owned by: james
owned by: myers
mutable description: M13 forward sequencing primer (46 bp upstream)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Ancient
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00106_sequence/1

T7
type: ComponentDefinition
persistent identity: BBa G00113
display id: BBa_G00113
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter sequencing primer, 20-mer
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: T7 promoter.
owned by: james
owned by: myers
mutable description: This primer anneals to the T7 Promoter region of any vector containing the T7 Promoter.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: <biblio> #Wallace pmid=6282692 </biblio>
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00113_sequence/1

BBa_G00600
type: ComponentDefinition
persistent identity: BBa G00600
display id: BBa_G00600
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0060
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00600_sequence/1

GFP-r
type: ComponentDefinition
persistent identity: BBa G00201
display id: BBa_G00201
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse primer for sequencing composite parts; binds to GFP (BBa_E0040)
creator: Bartholomew Canton
created: 2007-03-01T12:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a reverse primer that bind about midway through the GFP coding sequence and extends towards the start codon.
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00201_sequence/1

BBa_G00700
type: ComponentDefinition
persistent identity: BBa G00700
display id: BBa_G00700
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for I0500 (pBAD)
creator: Barry Canton
created: 2004-09-22T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Target sequence located approximately 400bp from the left end of pBAD. Suitable to sequence the final 800bp of pBAD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00700_sequence/1

M13-R(-26)
type: ComponentDefinition
persistent identity: BBa G00104
display id: BBa_G00104
version: 1
was generated by: iGEM to SBOL conversion
description: M13-R (-26)
created: 2006-06-07T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: Ancient
owned by: james
owned by: myers
mutable description: M13 reverse sequencing primer
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Ancient
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00104_sequence/1

M13-F(-40)
type: ComponentDefinition
persistent identity: BBa G00105
display id: BBa_G00105
version: 1
was generated by: iGEM to SBOL conversion
description: M13-F (-40)
created: 2006-06-07T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: Ancient
owned by: james
owned by: myers
mutable description: M13 forward sequencing primer, 40 bp upstream
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Ancient
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00105_sequence/1

T3
type: ComponentDefinition
persistent identity: BBa G00110
display id: BBa_G00110
version: 1
was generated by: iGEM to SBOL conversion
description: T3 promoter sequencing primer, 17-mer
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: T3 promoter.
owned by: james
owned by: myers
mutable description: This primer anneals to the T3 promoter region.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: <biblio> #McGraw pmid=3903658 </biblio>
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00110_sequence/1

CBDclos(T7
type: ComponentDefinition
persistent identity: BBa K863112
display id: BBa_K863112
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:18Z
mutable provenance: Cloning-vector
owned by: james
owned by: myers
mutable description: Cellulose binding domain of the [http://www.ncbi.nlm.nih.gov/nucleotide/327179207?report=genbank&log$=nucltop&blast_rank=3&RID=152ZCN0E01N Cellulomonas fimi ATCC 484 exoglucanase gene] in Freiburg-standard
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Added the regulatory unit BBa_K525998 (T7+RBS) in front of the CBD and a short GS-Linker behind the domain and used Freiburg-Standard to be able to fuse proteins to the domain.
star: false
creator: Moritz M??ller
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: B0034
sequence annotation: Cellulose Binding Protein
sequence annotation: RBS
sequence annotation: ATG
sequence annotation: BBa_K525998
sequence annotation: silent mutation (A original)
sequence annotation: GS-Linker
sequence annotation: TAA
sequence annotation: BBa_K863111
sequence annotation: AgeI
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K863112_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2188284
display id: annotation2188284
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188284/range2188284/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188284/range2188284/1
type: Range
persistent identity: range2188284
display id: range2188284
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 21
end: 32
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188290
display id: annotation2188290
version: 1
top level: CBDclos(T7
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188290/range2188290/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188290/range2188290/1
type: Range
persistent identity: range2188290
display id: range2188290
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 317
end: 322
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188285
display id: annotation2188285
version: 1
top level: CBDclos(T7
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188285/range2188285/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188285/range2188285/1
type: Range
persistent identity: range2188285
display id: range2188285
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 24
end: 27
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188287
display id: annotation2188287
version: 1
top level: CBDclos(T7
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188287/range2188287/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188287/range2188287/1
type: Range
persistent identity: range2188287
display id: range2188287
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 35
end: 38
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188283
display id: annotation2188283
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188283/range2188283/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188283/range2188283/1
type: Range
persistent identity: range2188283
display id: range2188283
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 1
end: 32
orientation: inline

silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200498
display id: annotation2200498
version: 1
top level: CBDclos(T7
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2200498/range2200498/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2200498/range2200498/1
type: Range
persistent identity: range2200498
display id: range2200498
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 112
end: 112
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188291
display id: annotation2188291
version: 1
top level: CBDclos(T7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188291/range2188291/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188291/range2188291/1
type: Range
persistent identity: range2188291
display id: range2188291
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 323
end: 328
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188293
display id: annotation2188293
version: 1
top level: CBDclos(T7
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188293/range2188293/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188293/range2188293/1
type: Range
persistent identity: range2188293
display id: range2188293
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 335
end: 337
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188286
display id: annotation2188286
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188286/range2188286/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188286/range2188286/1
type: Range
persistent identity: range2188286
display id: range2188286
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 35
end: 337
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188292
display id: annotation2188292
version: 1
top level: CBDclos(T7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188292/range2188292/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188292/range2188292/1
type: Range
persistent identity: range2188292
display id: range2188292
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 329
end: 334
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188289
display id: annotation2188289
version: 1
top level: CBDclos(T7
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188289/range2188289/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188289/range2188289/1
type: Range
persistent identity: range2188289
display id: range2188289
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 41
end: 316
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188288
display id: annotation2188288
version: 1
top level: CBDclos(T7
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188288/range2188288/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188288/range2188288/1
type: Range
persistent identity: range2188288
display id: range2188288
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 35
end: 40
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188282
display id: annotation2188282
version: 1
top level: CBDclos(T7
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188282/range2188282/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188282/range2188282/1
type: Range
persistent identity: range2188282
display id: range2188282
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_G00120
type: ComponentDefinition
persistent identity: BBa G00120
display id: BBa_G00120
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0012
creator: cconboy
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00120_sequence/1

BBa_G00401
type: ComponentDefinition
persistent identity: BBa G00401
display id: BBa_G00401
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0040
creator: cconboy
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00401_sequence/1

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
creator: Tom Knight
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
direction: direction/forward
top level: VF2
start: 1
end: 20
orientation: inline

T3
type: ComponentDefinition
persistent identity: BBa G00111
display id: BBa_G00111
version: 1
was generated by: iGEM to SBOL conversion
description: T3 promoter primer, 20-mer
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: T3 promoter
owned by: james
owned by: myers
mutable description: This primer is complementary to the consensus sequence in all Class III (1) promoters for T3 RNA Polymerase.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: <biblio> #McGraw pmid=3903658 </biblio>
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00111_sequence/1

BioBrick-r
type: ComponentDefinition
persistent identity: BBa G1005
display id: BBa_G1005
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick suffix primer for amplifying BioBrick parts (BioBrick-r)
creator: Reshma Shetty
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Reverse primer that binds to the BioBrick suffix. It is useful for amplifying BioBrick parts.
mutable notes: It includes extra bases on the 5' end to enable both efficient cutting by EcoRI and 3' A addition by Taq polymerase.
mutable provenance: The BioBrick suffix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G1005_sequence/1

BBa_G00501
type: ComponentDefinition
persistent identity: BBa G00501
display id: BBa_G00501
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0050
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00501_sequence/1

SP6
type: ComponentDefinition
persistent identity: BBa G00109
display id: BBa_G00109
version: 1
was generated by: iGEM to SBOL conversion
description: SP6 promoter sequencing primer, 24-mer
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: SP6 RNA Polymerase promoter
owned by: james
owned by: myers
mutable description: This primer is complementary to a portion of the SP6 RNA Polymerase promoter and can be used to sequence DNA inserted in the polylinker regions of common cloning vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: <biblio> #Melton pmid=6091052 #Chen Chen, E. et al. (1984) DNA, 4, 165-170. </biblio>
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00109_sequence/1

CBDclos(J6
type: ComponentDefinition
persistent identity: BBa K863114
display id: BBa_K863114
version: 1
was generated by: iGEM to SBOL conversion
description: CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP
creator: Moritz M??ller
created: 2012-09-22T11:00:00Z
modified: 2015-06-17T01:20:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene (cbp A)] with a constitutive promoter and RBS (<partinfo>J61101</partinfo>) and a fused-GFP protein with His-tag.
mutable notes: Was made to switch from a T7-promoter to a constitutive promoter
mutable provenance: Cellulose binding domain form a cloning-vector. <partinfo>J61101</partinfo> and [http://partsregistry.org/Part:BBa_I13522 GFP] from the partsregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Freiburg-Scar
sequence annotation: Cellulose Binding Protein
sequence annotation: BBa_K863121
sequence annotation: BBa_J23100
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: GS-Linker
sequence annotation: BBa_J61101
sequence annotation: AgeI
sequence annotation: His-Tag
sequence annotation: NgoMIV
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: BBa_K863111
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K863114_sequence/1

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2194138
display id: annotation2194138
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1
type: Range
persistent identity: range2194138
display id: range2194138
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 371
end: 376
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194134
display id: annotation2194134
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1
type: Range
persistent identity: range2194134
display id: range2194134
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 71
end: 82
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2194139
display id: annotation2194139
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1
type: Range
persistent identity: range2194139
display id: range2194139
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 377
end: 1117
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2194129
display id: annotation2194129
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1
type: Range
persistent identity: range2194129
display id: range2194129
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 1
end: 35
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2194143
display id: annotation2194143
version: 1
top level: CBDclos(J6
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1
type: Range
persistent identity: range2194143
display id: range2194143
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1115
end: 1117
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2194132
display id: annotation2194132
version: 1
top level: CBDclos(J6
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1
type: Range
persistent identity: range2194132
display id: range2194132
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 62
end: 64
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2194137
display id: annotation2194137
version: 1
top level: CBDclos(J6
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1
type: Range
persistent identity: range2194137
display id: range2194137
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 365
end: 370
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2194130
display id: annotation2194130
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1
type: Range
persistent identity: range2194130
display id: range2194130
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 44
end: 55
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2194142
display id: annotation2194142
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1
type: Range
persistent identity: range2194142
display id: range2194142
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1109
end: 1114
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2194141
display id: annotation2194141
version: 1
top level: CBDclos(J6
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1
type: Range
persistent identity: range2194141
display id: range2194141
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1091
end: 1108
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2194133
display id: annotation2194133
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1
type: Range
persistent identity: range2194133
display id: range2194133
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 65
end: 70
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194136
display id: annotation2194136
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1
type: Range
persistent identity: range2194136
display id: range2194136
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 359
end: 364
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2194135
display id: annotation2194135
version: 1
top level: CBDclos(J6
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1
type: Range
persistent identity: range2194135
display id: range2194135
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 83
end: 358
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2194131
display id: annotation2194131
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1
type: Range
persistent identity: range2194131
display id: range2194131
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 62
end: 370
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2194140
display id: annotation2194140
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1
type: Range
persistent identity: range2194140
display id: range2194140
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 377
end: 1090
orientation: inline

Prefix-R
type: ComponentDefinition
persistent identity: BBa G1001
display id: BBa_G1001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)
creator: Jason Kelly
created: 2008-04-21T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the REV primer used in the measurement kit to prepare pSB3K3
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G1001_sequence/1

BBa_G00510
type: ComponentDefinition
persistent identity: BBa G00510
display id: BBa_G00510
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0051
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00510_sequence/1

BBa_K863113
type: ComponentDefinition
persistent identity: BBa K863113
display id: BBa_K863113
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:29Z
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
mutable description: Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] with T7, RBS linked with two amino acids (GS) to a GFP with His-tag.
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: CBDclos fused to a his-tagged GFP in a Freiburg-manner
star: false
creator: Moritz M??ller
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RBS
sequence annotation: ATG
sequence annotation: BBa_K525998
sequence annotation: BBa_K863111
sequence annotation: B0034
sequence annotation: Freiburg-Scar
sequence annotation: Cellulose Binding Protein
sequence annotation: TAA
sequence annotation: BBa_K863112
sequence annotation: Silent mutation (A original)
sequence annotation: T7 promoter
sequence annotation: AgeI
sequence annotation: GS-Linker
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: His-Tag
sequence annotation: BBa_K863121
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K863113_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2188390
display id: annotation2188390
version: 1
top level: BBa_K863113
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1
type: Range
persistent identity: range2188390
display id: range2188390
version: 1
top level: BBa_K863113
direction: direction/forward
start: 24
end: 27
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188391
display id: annotation2188391
version: 1
top level: BBa_K863113
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1
type: Range
persistent identity: range2188391
display id: range2188391
version: 1
top level: BBa_K863113
direction: direction/forward
start: 35
end: 38
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188387
display id: annotation2188387
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1
type: Range
persistent identity: range2188387
display id: range2188387
version: 1
top level: BBa_K863113
direction: direction/forward
start: 1
end: 32
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188393
display id: annotation2188393
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1
type: Range
persistent identity: range2188393
display id: range2188393
version: 1
top level: BBa_K863113
direction: direction/forward
start: 35
end: 328
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188389
display id: annotation2188389
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1
type: Range
persistent identity: range2188389
display id: range2188389
version: 1
top level: BBa_K863113
direction: direction/forward
start: 21
end: 32
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188397
display id: annotation2188397
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1
type: Range
persistent identity: range2188397
display id: range2188397
version: 1
top level: BBa_K863113
direction: direction/forward
start: 329
end: 334
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188392
display id: annotation2188392
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1
type: Range
persistent identity: range2188392
display id: range2188392
version: 1
top level: BBa_K863113
direction: direction/forward
start: 35
end: 40
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188402
display id: annotation2188402
version: 1
top level: BBa_K863113
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1
type: Range
persistent identity: range2188402
display id: range2188402
version: 1
top level: BBa_K863113
direction: direction/forward
start: 1073
end: 1075
orientation: inline

BBa_K863112
type: SequenceAnnotation
persistent identity: annotation2188386
display id: annotation2188386
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1
type: Range
persistent identity: range2188386
display id: range2188386
version: 1
top level: BBa_K863113
direction: direction/reverse
start: 1
end: 328
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188395
display id: annotation2188395
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1
type: Range
persistent identity: range2188395
display id: range2188395
version: 1
top level: BBa_K863113
direction: direction/forward
start: 317
end: 322
orientation: inline

Silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200501
display id: annotation2200501
version: 1
top level: BBa_K863113
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1
type: Range
persistent identity: range2200501
display id: range2200501
version: 1
top level: BBa_K863113
direction: direction/forward
start: 112
end: 112
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188388
display id: annotation2188388
version: 1
top level: BBa_K863113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1
type: Range
persistent identity: range2188388
display id: range2188388
version: 1
top level: BBa_K863113
direction: direction/forward
start: 1
end: 20
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188401
display id: annotation2188401
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1
type: Range
persistent identity: range2188401
display id: range2188401
version: 1
top level: BBa_K863113
direction: direction/forward
start: 1068
end: 1072
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188396
display id: annotation2188396
version: 1
top level: BBa_K863113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1
type: Range
persistent identity: range2188396
display id: range2188396
version: 1
top level: BBa_K863113
direction: direction/forward
start: 323
end: 328
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188394
display id: annotation2188394
version: 1
top level: BBa_K863113
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1
type: Range
persistent identity: range2188394
display id: range2188394
version: 1
top level: BBa_K863113
direction: direction/forward
start: 41
end: 316
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188400
display id: annotation2188400
version: 1
top level: BBa_K863113
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1
type: Range
persistent identity: range2188400
display id: range2188400
version: 1
top level: BBa_K863113
direction: direction/forward
start: 1049
end: 1066
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188398
display id: annotation2188398
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1
type: Range
persistent identity: range2188398
display id: range2188398
version: 1
top level: BBa_K863113
direction: direction/reverse
start: 335
end: 1075
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188399
display id: annotation2188399
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1
type: Range
persistent identity: range2188399
display id: range2188399
version: 1
top level: BBa_K863113
direction: direction/forward
start: 335
end: 1048
orientation: inline

BBa_G00521
type: ComponentDefinition
persistent identity: BBa G00521
display id: BBa_G00521
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0052
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00521_sequence/1

M13-F(-20)
type: ComponentDefinition
persistent identity: BBa G00103
display id: BBa_G00103
version: 1
was generated by: iGEM to SBOL conversion
description: M13-F (-20)
created: 2006-06-07T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: Ancient
owned by: james
owned by: myers
mutable description: M13 forward sequencing primer
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Ancient
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00103_sequence/1

BBa_G00601
type: ComponentDefinition
persistent identity: BBa G00601
display id: BBa_G00601
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0060
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00601_sequence/1

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863103
display id: BBa_K863103
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:19:26Z
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
mutable description: Cellulose binding Domain of C. Fimi Exoglucanase gene with T7, RBS linked with two amino acids (GS) to a GFP (Freiburg-Standard)
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: CBDcex fused to a his-tagged GFP in a Freiburg-manner
star: false
creator: Moritz M??ller
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GS-Linker
sequence annotation: ATG
sequence annotation: Exoglucanase
sequence annotation: Freiburg-Scar
sequence annotation: RBS
sequence annotation: BBa_K525998
sequence annotation: B0034
sequence annotation: AgeI
sequence annotation: T7 promoter
sequence annotation: BBa_K863102
sequence annotation: BBa_E0040
sequence annotation: BBa_K863101
sequence annotation: His-Tag
sequence annotation: TAA
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: BBa_K863121
sequence: https://synbiohub.org/public/igem/BBa_K863103_sequence/1

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188022
display id: annotation2188022
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1
type: Range
persistent identity: range2188022
display id: range2188022
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 359
end: 364
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188017
display id: annotation2188017
version: 1
top level: CBDcex(T7)
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1
type: Range
persistent identity: range2188017
display id: range2188017
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 35
end: 37
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188021
display id: annotation2188021
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1
type: Range
persistent identity: range2188021
display id: range2188021
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 350
end: 358
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188023
display id: annotation2188023
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1
type: Range
persistent identity: range2188023
display id: range2188023
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 365
end: 370
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188016
display id: annotation2188016
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1
type: Range
persistent identity: range2188016
display id: range2188016
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 24
end: 27
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188013
display id: annotation2188013
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1
type: Range
persistent identity: range2188013
display id: range2188013
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1
end: 32
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188015
display id: annotation2188015
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1
type: Range
persistent identity: range2188015
display id: range2188015
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 21
end: 32
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188027
display id: annotation2188027
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1
type: Range
persistent identity: range2188027
display id: range2188027
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1103
end: 1108
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188012
display id: annotation2188012
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1
type: Range
persistent identity: range2188012
display id: range2188012
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1
end: 20
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188018
display id: annotation2188018
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1
type: Range
persistent identity: range2188018
display id: range2188018
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 38
end: 49
orientation: inline

BBa_K863102
type: SequenceAnnotation
persistent identity: annotation2188014
display id: annotation2188014
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1
type: Range
persistent identity: range2188014
display id: range2188014
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1
end: 364
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188024
display id: annotation2188024
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1
type: Range
persistent identity: range2188024
display id: range2188024
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 371
end: 1084
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2188019
display id: annotation2188019
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1
type: Range
persistent identity: range2188019
display id: range2188019
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 38
end: 364
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188026
display id: annotation2188026
version: 1
top level: CBDcex(T7)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1
type: Range
persistent identity: range2188026
display id: range2188026
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1085
end: 1102
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188028
display id: annotation2188028
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1
type: Range
persistent identity: range2188028
display id: range2188028
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1109
end: 1111
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2188020
display id: annotation2188020
version: 1
top level: CBDcex(T7)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1
type: Range
persistent identity: range2188020
display id: range2188020
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 50
end: 349
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188025
display id: annotation2188025
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1
type: Range
persistent identity: range2188025
display id: range2188025
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 371
end: 1111
orientation: inline

M13-R(-46)
type: ComponentDefinition
persistent identity: BBa G00107
display id: BBa_G00107
version: 1
was generated by: iGEM to SBOL conversion
description: M13-R (-46)
created: 2006-06-07T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: Ancient
owned by: james
owned by: myers
mutable description: M13 reverse primer (46 bp upstream)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Ancient
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00107_sequence/1

Prefix-R
type: ComponentDefinition
persistent identity: BBa G1003
display id: BBa_G1003
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)
creator: Reshma Shetty
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The primer sequence that binds to the BioBrick prefix for PCR amplification of BioBrick plasmid backbones. This sequence is used by Meagan Lizarazo at the Registry.
mutable notes: This primer sequence is 1 nucleotide shorter than BBa_G1001.
mutable provenance: The BioBrick prefix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G1003_sequence/1

CBDcex(J61
type: ComponentDefinition
persistent identity: BBa K863104
display id: BBa_K863104
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding Domain from C. Fimi with const. Promotor and GFP
creator: Moritz M??ller
created: 2012-09-20T11:00:00Z
modified: 2015-06-17T01:19:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Cellulomonas Fimi with const. Promotor J61101 and Reporter GFP
mutable notes: a switch to a const. Promoter
mutable provenance: different
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Freiburg-Scar
sequence annotation: BBa_J61101
sequence annotation: TAA
sequence annotation: BBa_E0040
sequence annotation: BBa_K863121
sequence annotation: AgeI
sequence annotation: ATG
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: GS-Linker
sequence annotation: Exoglucanase
sequence annotation: BBa_J23100
sequence annotation: His-Tag
sequence annotation: BBa_K863101
sequence annotation: NgoMIV
sequence: https://synbiohub.org/public/igem/BBa_K863104_sequence/1

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2189492
display id: annotation2189492
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1
type: Range
persistent identity: range2189492
display id: range2189492
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 398
end: 403
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2189484
display id: annotation2189484
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1
type: Range
persistent identity: range2189484
display id: range2189484
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 44
end: 55
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2189497
display id: annotation2189497
version: 1
top level: CBDcex(J61
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1
type: Range
persistent identity: range2189497
display id: range2189497
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1142
end: 1144
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2189494
display id: annotation2189494
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1
type: Range
persistent identity: range2189494
display id: range2189494
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1117
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2189493
display id: annotation2189493
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1
type: Range
persistent identity: range2189493
display id: range2189493
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1144
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2189496
display id: annotation2189496
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1
type: Range
persistent identity: range2189496
display id: range2189496
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1136
end: 1141
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2189485
display id: annotation2189485
version: 1
top level: CBDcex(J61
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1
type: Range
persistent identity: range2189485
display id: range2189485
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 64
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2189489
display id: annotation2189489
version: 1
top level: CBDcex(J61
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1
type: Range
persistent identity: range2189489
display id: range2189489
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 83
end: 382
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2189491
display id: annotation2189491
version: 1
top level: CBDcex(J61
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1
type: Range
persistent identity: range2189491
display id: range2189491
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 392
end: 397
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189488
display id: annotation2189488
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1
type: Range
persistent identity: range2189488
display id: range2189488
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 71
end: 82
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189483
display id: annotation2189483
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1
type: Range
persistent identity: range2189483
display id: range2189483
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1
end: 35
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2189495
display id: annotation2189495
version: 1
top level: CBDcex(J61
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1
type: Range
persistent identity: range2189495
display id: range2189495
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1118
end: 1135
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2189486
display id: annotation2189486
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1
type: Range
persistent identity: range2189486
display id: range2189486
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 397
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2189487
display id: annotation2189487
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1
type: Range
persistent identity: range2189487
display id: range2189487
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 65
end: 70
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189490
display id: annotation2189490
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1
type: Range
persistent identity: range2189490
display id: range2189490
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 383
end: 391
orientation: inline

BBa_G00121
type: ComponentDefinition
persistent identity: BBa G00121
display id: BBa_G00121
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0012
creator: cconboy
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00121_sequence/1

CFP/YFP-f
type: ComponentDefinition
persistent identity: BBa G00200
display id: BBa_G00200
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing composite parts; binds to CFP/YFP (BBa_E0020/22, BBa_E0030/32)
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00200_sequence/1

T3
type: ComponentDefinition
persistent identity: BBa G00112
display id: BBa_G00112
version: 1
was generated by: iGEM to SBOL conversion
description: T3 promoter sequencing primer, 24-mer
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: T3 promoter
owned by: james
owned by: myers
mutable description: This primer anneals to the T3 promoter region.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: <biblio> #McGraw pmid=3903658 </biblio>
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00112_sequence/1

BBa_G00530
type: ComponentDefinition
persistent identity: BBa G00530
display id: BBa_G00530
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0053
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00530_sequence/1

BioBrick-f
type: ComponentDefinition
persistent identity: BBa G1004
display id: BBa_G1004
version: 1
was generated by: iGEM to SBOL conversion
description: Forward BioBrick prefix primer for amplifying BioBrick parts (BioBrick-f)
creator: Reshma Shetty
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Forward primer that binds to the BioBrick prefix. It is useful for amplifying BioBrick parts.
mutable notes: It includes extra bases on the 5' end to enable both efficient cutting by EcoRI and 3' A addition by Taq polymerase.
mutable provenance: The BioBrick prefix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G1004_sequence/1

BBa_G00400
type: ComponentDefinition
persistent identity: BBa G00400
display id: BBa_G00400
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0040
creator: cconboy
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00400_sequence/1

BBa_G00701
type: ComponentDefinition
persistent identity: BBa G00701
display id: BBa_G00701
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse primer for I0500 (pBAD)
creator: Barry Canton
created: 2004-09-22T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Target sequence located approximately 400bp from the right end of I0500. Suitable for sequencing the first 800bp of pBAD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00701_sequence/1

Suffix-F
type: ComponentDefinition
persistent identity: BBa G1000
display id: BBa_G1000
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)
creator: Jason Kelly
created: 2008-04-21T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the FWD primer used in the measurement kit to amplify pSB3K3
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G1000_sequence/1

BBa_G00520
type: ComponentDefinition
persistent identity: BBa G00520
display id: BBa_G00520
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0052
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00520_sequence/1

BBa_G00511
type: ComponentDefinition
persistent identity: BBa G00511
display id: BBa_G00511
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0051
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00511_sequence/1

VR
type: ComponentDefinition
persistent identity: BBa G00101
display id: BBa_G00101
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse primer for sequencing/amplifying BioBrick parts (VR)
creator: Tom Knight
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Originally named VR, This reverse primer binds downstream of the standard BBa_MSC on biobrick vectors. Used for general-purpose PCR and sequencing of Biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00101_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1934519
display id: annotation1934519
version: 1
top level: VR
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_G00101/annotation1934519/range1934519/1

https://synbiohub.org/public/igem/BBa_G00101/annotation1934519/range1934519/1
type: Range
persistent identity: range1934519
display id: range1934519
version: 1
direction: direction/forward
top level: VR
start: 1
end: 20
orientation: inline

BBa_G00531
type: ComponentDefinition
persistent identity: BBa G00531
display id: BBa_G00531
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0053
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00531_sequence/1

BBa_G00500
type: ComponentDefinition
persistent identity: BBa G00500
display id: BBa_G00500
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0050
creator: cconboy
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00500_sequence/1

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863102
display id: BBa_K863102
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
created: 2012-09-18T11:00:00Z
modified: 2015-06-17T01:19:09Z
mutable provenance: Cloning-vector
owned by: james
owned by: myers
mutable description: Cellulose binding domain of the [http://www.ncbi.nlm.nih.gov/nucleotide/327179207?report=genbank&log$=nucltop&blast_rank=3&RID=152ZCN0E01N Cellulomonas fimi ATCC 484 exoglucanase gene] in Freiburg-standard with T7, RBS, GS-Linker (Freiburg-Standard)
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: You can find additional info to the Cellulose binding domain here: [http://partsregistry.org/Part:BBa_K863101:Design CBDcex_Freiburg]
star: false
creator: Moritz M??ller
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RBS
sequence annotation: BBa_K525998
sequence annotation: B0034
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: Exoglucanase
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: T7 promoter
sequence annotation: AgeI
sequence annotation: BBa_K863101
sequence annotation: GS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K863102_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2187506
display id: annotation2187506
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187506/range2187506/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187506/range2187506/1
type: Range
persistent identity: range2187506
display id: range2187506
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 24
end: 27
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2187504
display id: annotation2187504
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187504/range2187504/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187504/range2187504/1
type: Range
persistent identity: range2187504
display id: range2187504
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1
end: 32
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2187505
display id: annotation2187505
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187505/range2187505/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187505/range2187505/1
type: Range
persistent identity: range2187505
display id: range2187505
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 21
end: 32
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2187510
display id: annotation2187510
version: 1
top level: CBDcex(T7)
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187510/range2187510/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187510/range2187510/1
type: Range
persistent identity: range2187510
display id: range2187510
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 50
end: 349
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2187508
display id: annotation2187508
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187508/range2187508/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187508/range2187508/1
type: Range
persistent identity: range2187508
display id: range2187508
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 38
end: 49
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2187514
display id: annotation2187514
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187514/range2187514/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187514/range2187514/1
type: Range
persistent identity: range2187514
display id: range2187514
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 371
end: 373
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2187507
display id: annotation2187507
version: 1
top level: CBDcex(T7)
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187507/range2187507/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187507/range2187507/1
type: Range
persistent identity: range2187507
display id: range2187507
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 35
end: 37
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2187511
display id: annotation2187511
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187511/range2187511/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187511/range2187511/1
type: Range
persistent identity: range2187511
display id: range2187511
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 350
end: 358
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2187503
display id: annotation2187503
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187503/range2187503/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187503/range2187503/1
type: Range
persistent identity: range2187503
display id: range2187503
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1
end: 20
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2187513
display id: annotation2187513
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187513/range2187513/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187513/range2187513/1
type: Range
persistent identity: range2187513
display id: range2187513
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 365
end: 370
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2187509
display id: annotation2187509
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187509/range2187509/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187509/range2187509/1
type: Range
persistent identity: range2187509
display id: range2187509
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 38
end: 373
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2187512
display id: annotation2187512
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187512/range2187512/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187512/range2187512/1
type: Range
persistent identity: range2187512
display id: range2187512
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 359
end: 364
orientation: inline

Suffix-F
type: ComponentDefinition
persistent identity: BBa G1002
display id: BBa_G1002
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)
creator: Reshma Shetty
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The primer sequence that binds to the BioBrick suffix for PCR amplification of BioBrick plasmid backbones. This sequence is used by Meagan Lizarazo at the Registry.
mutable notes: This primer sequence is 1 nucleotide shorter than BBa_G1000.
mutable provenance: The BioBrick suffix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G1002_sequence/1

https://synbiohub.org/public/igem/BBa_G00112_sequence/1
type: Sequence
persistent identity: BBa G00112 sequence
display id: BBa_G00112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: gcgcgaaattaaccctcactaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863113_sequence/1
type: Sequence
persistent identity: BBa K863113 sequence
display id: BBa_K863113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaataatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggcatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacatcaccatcaccatcacaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00106_sequence/1
type: Sequence
persistent identity: BBa G00106 sequence
display id: BBa_G00106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccagggttttcccagtcacga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00107_sequence/1
type: Sequence
persistent identity: BBa G00107 sequence
display id: BBa_G00107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagcggataacaatttcacacagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00104_sequence/1
type: Sequence
persistent identity: BBa G00104 sequence
display id: BBa_G00104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caggaaacagctatgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863103_sequence/1
type: Sequence
persistent identity: BBa K863103 sequence
display id: BBa_K863103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaataatgggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagcaccggcatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacatcaccatcaccatcacaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00111_sequence/1
type: Sequence
persistent identity: BBa G00111 sequence
display id: BBa_G00111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaccctcactaaaggga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00110_sequence/1
type: Sequence
persistent identity: BBa G00110 sequence
display id: BBa_G00110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaccctcactaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00103_sequence/1
type: Sequence
persistent identity: BBa G00103 sequence
display id: BBa_G00103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtaaaacgacggccagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863102_sequence/1
type: Sequence
persistent identity: BBa K863102 sequence
display id: BBa_K863102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaataatgggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagcaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00105_sequence/1
type: Sequence
persistent identity: BBa G00105 sequence
display id: BBa_G00105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: gttttcccagtcacgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00113_sequence/1
type: Sequence
persistent identity: BBa G00113 sequence
display id: BBa_G00113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactataggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863112_sequence/1
type: Sequence
persistent identity: BBa K863112 sequence
display id: BBa_K863112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaataatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00109_sequence/1
type: Sequence
persistent identity: BBa G00109 sequence
display id: BBa_G00109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catacgatttaggtgacactatag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z