Reverse primer for sequencing/amplifying BioBrick parts (VR)
Originally named VR, This reverse primer binds downstream of the standard BBa_MSC on biobrick vectors. Used for general-purpose PCR and sequencing of Biobrick parts.
Target sequence located approximately 400bp from the left end of pBAD. Suitable to sequence the final 800bp of pBAD
Forward BioBrick prefix primer for amplifying BioBrick parts (BioBrick-f)
Forward primer that binds to the BioBrick prefix. It is useful for amplifying BioBrick parts.
It includes extra bases on the 5' end to enable both efficient cutting by EcoRI and 3' A addition by Taq polymerase.
Reverse BioBrick suffix primer for amplifying BioBrick parts (BioBrick-r)
Reverse primer that binds to the BioBrick suffix. It is useful for amplifying BioBrick parts.
Target sequence located approximately 400bp from the right end of I0500. Suitable for sequencing the first 800bp of pBAD
Forward primer for sequencing/amplifying BioBrick parts (VF2)
Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
Conversion of the iGEM parts registry to SBOL2.1