//primer/part/sequencing
type: Collection
persistent identity: primer part sequencing
display id: primer_part_sequencing
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_G00400
member: BBa_G00121
member: BBa_G00701
member: BBa_G00401
member: BBa_G00700
member: VF2
member: VR

//primer/part
type: Collection
persistent identity: primer part
display id: primer_part
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //primer/part/sequencing
member: //primer/part/amplification

//primer/part/amplification
type: Collection
persistent identity: primer part amplification
display id: primer_part_amplification
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_G00511
member: BioBrick-r
member: BBa_G00121
member: BBa_G00401
member: BBa_G00500
member: BBa_G00530
member: VF2
member: BBa_G00520
member: BBa_G00400
member: BBa_G00531
member: BioBrick-f
member: BBa_G00501
member: BBa_G00600
member: BBa_G00120
member: BBa_G00601
member: BBa_G00700
member: BBa_G00521
member: VR
member: BBa_G00510

VR
type: ComponentDefinition
persistent identity: BBa G00101
display id: BBa_G00101
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse primer for sequencing/amplifying BioBrick parts (VR)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VR, This reverse primer binds downstream of the standard BBa_MSC on biobrick vectors. Used for general-purpose PCR and sequencing of Biobrick parts.
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00101_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1934519
display id: annotation1934519
version: 1
top level: VR
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_G00101/annotation1934519/range1934519/1

https://synbiohub.org/public/igem/BBa_G00101/annotation1934519/range1934519/1
type: Range
persistent identity: range1934519
display id: range1934519
version: 1
top level: VR
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_G00121
type: ComponentDefinition
persistent identity: BBa G00121
display id: BBa_G00121
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0012
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00121_sequence/1

BBa_G00601
type: ComponentDefinition
persistent identity: BBa G00601
display id: BBa_G00601
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0060
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00601_sequence/1

BBa_G00501
type: ComponentDefinition
persistent identity: BBa G00501
display id: BBa_G00501
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0050
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00501_sequence/1

BBa_G00510
type: ComponentDefinition
persistent identity: BBa G00510
display id: BBa_G00510
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0051
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00510_sequence/1

BBa_G00520
type: ComponentDefinition
persistent identity: BBa G00520
display id: BBa_G00520
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0052
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00520_sequence/1

BBa_G00401
type: ComponentDefinition
persistent identity: BBa G00401
display id: BBa_G00401
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0040
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00401_sequence/1

BBa_G00700
type: ComponentDefinition
persistent identity: BBa G00700
display id: BBa_G00700
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for I0500 (pBAD)
created: 2004-09-22T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Target sequence located approximately 400bp from the left end of pBAD. Suitable to sequence the final 800bp of pBAD
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00700_sequence/1

BioBrick-f
type: ComponentDefinition
persistent identity: BBa G1004
display id: BBa_G1004
version: 1
was generated by: iGEM to SBOL conversion
description: Forward BioBrick prefix primer for amplifying BioBrick parts (BioBrick-f)
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: The BioBrick prefix.
owned by: james
owned by: myers
mutable description: Forward primer that binds to the BioBrick prefix. It is useful for amplifying BioBrick parts.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It includes extra bases on the 5' end to enable both efficient cutting by EcoRI and 3' A addition by Taq polymerase.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G1004_sequence/1

BBa_G00531
type: ComponentDefinition
persistent identity: BBa G00531
display id: BBa_G00531
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0053
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00531_sequence/1

BBa_G00600
type: ComponentDefinition
persistent identity: BBa G00600
display id: BBa_G00600
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0060
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00600_sequence/1

BBa_G00500
type: ComponentDefinition
persistent identity: BBa G00500
display id: BBa_G00500
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0050
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00500_sequence/1

BBa_G00530
type: ComponentDefinition
persistent identity: BBa G00530
display id: BBa_G00530
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0053
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00530_sequence/1

BioBrick-r
type: ComponentDefinition
persistent identity: BBa G1005
display id: BBa_G1005
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick suffix primer for amplifying BioBrick parts (BioBrick-r)
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: The BioBrick suffix.
owned by: james
owned by: myers
mutable description: Reverse primer that binds to the BioBrick suffix. It is useful for amplifying BioBrick parts.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It includes extra bases on the 5' end to enable both efficient cutting by EcoRI and 3' A addition by Taq polymerase.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G1005_sequence/1

BBa_G00511
type: ComponentDefinition
persistent identity: BBa G00511
display id: BBa_G00511
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0051
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00511_sequence/1

BBa_G00120
type: ComponentDefinition
persistent identity: BBa G00120
display id: BBa_G00120
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0012
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00120_sequence/1

BBa_G00701
type: ComponentDefinition
persistent identity: BBa G00701
display id: BBa_G00701
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse primer for I0500 (pBAD)
created: 2004-09-22T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Target sequence located approximately 400bp from the right end of I0500. Suitable for sequencing the first 800bp of pBAD
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00701_sequence/1

BBa_G00521
type: ComponentDefinition
persistent identity: BBa G00521
display id: BBa_G00521
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse Primer to C0052
created: 2004-06-01T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00521_sequence/1

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_G00400
type: ComponentDefinition
persistent identity: BBa G00400
display id: BBa_G00400
version: 1
was generated by: iGEM to SBOL conversion
description: Forward Primer to C0040
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00400_sequence/1

https://synbiohub.org/public/igem/BBa_G00101_sequence/1
type: Sequence
persistent identity: BBa G00101 sequence
display id: BBa_G00101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaccgcctttgagtgagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00530_sequence/1
type: Sequence
persistent identity: BBa G00530 sequence
display id: BBa_G00530_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaggtgaaaacgaggccacattc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G1005_sequence/1
type: Sequence
persistent identity: BBa G1005 sequence
display id: BBa_G1005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttcttcctgcagcggccgctactagta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00600_sequence/1
type: Sequence
persistent identity: BBa G00600 sequence
display id: BBa_G00600_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtacagcgtgctgaatatgaggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00701_sequence/1
type: Sequence
persistent identity: BBa G00701 sequence
display id: BBa_G00701_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccaacggttatctcgattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00501_sequence/1
type: Sequence
persistent identity: BBa G00501 sequence
display id: BBa_G00501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgcctaatgaggactttggctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00511_sequence/1
type: Sequence
persistent identity: BBa G00511 sequence
display id: BBa_G00511_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cactgactagcgataactttccccac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00521_sequence/1
type: Sequence
persistent identity: BBa G00521 sequence
display id: BBa_G00521_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctacgaattttaccctcgcttcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00601_sequence/1
type: Sequence
persistent identity: BBa G00601 sequence
display id: BBa_G00601_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgattgatgccctggagtatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00120_sequence/1
type: Sequence
persistent identity: BBa G00120 sequence
display id: BBa_G00120_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcttgctgcaactctctcaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00400_sequence/1
type: Sequence
persistent identity: BBa G00400 sequence
display id: BBa_G00400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cacctactactgatagtatgccgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00531_sequence/1
type: Sequence
persistent identity: BBa G00531 sequence
display id: BBa_G00531_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctggaagatttgcgagttttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G1004_sequence/1
type: Sequence
persistent identity: BBa G1004 sequence
display id: BBa_G1004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttcttcgaattcgcggccgcttctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00121_sequence/1
type: Sequence
persistent identity: BBa G00121 sequence
display id: BBa_G00121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagtcgggaaacctgtcgtg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00510_sequence/1
type: Sequence
persistent identity: BBa G00510 sequence
display id: BBa_G00510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatttctgcatagccagacttggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00700_sequence/1
type: Sequence
persistent identity: BBa G00700 sequence
display id: BBa_G00700_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatttatcgccagcagctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00520_sequence/1
type: Sequence
persistent identity: BBa G00520 sequence
display id: BBa_G00520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggtcatagatggcggtcagaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00500_sequence/1
type: Sequence
persistent identity: BBa G00500 sequence
display id: BBa_G00500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggaaaacaatgcacgttaagcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00401_sequence/1
type: Sequence
persistent identity: BBa G00401 sequence
display id: BBa_G00401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggacccactttcacatttaag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z