//primer/t7
type: Collection
persistent identity: primer t7
display id: primer_t7
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: CBDcex(T7)
member: BBa_K863113
member: CBDclos(T7
member: T7

CBDclos(T7
type: ComponentDefinition
persistent identity: BBa K863112
display id: BBa_K863112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding domain of the [http://www.ncbi.nlm.nih.gov/nucleotide/327179207?report=genbank&log$=nucltop&blast_rank=3&RID=152ZCN0E01N Cellulomonas fimi ATCC 484 exoglucanase gene] in Freiburg-standard
mutable notes: Added the regulatory unit BBa_K525998 (T7+RBS) in front of the CBD and a short GS-Linker behind the domain and used Freiburg-Standard to be able to fuse proteins to the domain.
mutable provenance: Cloning-vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K863111
sequence annotation: BBa_K525998
sequence annotation: ATG
sequence annotation: Cellulose Binding Protein
sequence annotation: RBS
sequence annotation: GS-Linker
sequence annotation: T7 promoter
sequence annotation: silent mutation (A original)
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K863112_sequence/1

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188286
display id: annotation2188286
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188286/range2188286/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188286/range2188286/1
type: Range
persistent identity: range2188286
display id: range2188286
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 35
end: 337
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188283
display id: annotation2188283
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188283/range2188283/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188283/range2188283/1
type: Range
persistent identity: range2188283
display id: range2188283
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 1
end: 32
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188287
display id: annotation2188287
version: 1
top level: CBDclos(T7
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188287/range2188287/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188287/range2188287/1
type: Range
persistent identity: range2188287
display id: range2188287
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 35
end: 38
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188290
display id: annotation2188290
version: 1
top level: CBDclos(T7
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188290/range2188290/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188290/range2188290/1
type: Range
persistent identity: range2188290
display id: range2188290
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 317
end: 322
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188285
display id: annotation2188285
version: 1
top level: CBDclos(T7
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188285/range2188285/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188285/range2188285/1
type: Range
persistent identity: range2188285
display id: range2188285
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 24
end: 27
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188288
display id: annotation2188288
version: 1
top level: CBDclos(T7
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188288/range2188288/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188288/range2188288/1
type: Range
persistent identity: range2188288
display id: range2188288
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 35
end: 40
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188291
display id: annotation2188291
version: 1
top level: CBDclos(T7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188291/range2188291/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188291/range2188291/1
type: Range
persistent identity: range2188291
display id: range2188291
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 323
end: 328
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188282
display id: annotation2188282
version: 1
top level: CBDclos(T7
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188282/range2188282/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188282/range2188282/1
type: Range
persistent identity: range2188282
display id: range2188282
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 1
end: 20
orientation: inline

silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200498
display id: annotation2200498
version: 1
top level: CBDclos(T7
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2200498/range2200498/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2200498/range2200498/1
type: Range
persistent identity: range2200498
display id: range2200498
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 112
end: 112
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188292
display id: annotation2188292
version: 1
top level: CBDclos(T7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188292/range2188292/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188292/range2188292/1
type: Range
persistent identity: range2188292
display id: range2188292
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 329
end: 334
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188293
display id: annotation2188293
version: 1
top level: CBDclos(T7
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188293/range2188293/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188293/range2188293/1
type: Range
persistent identity: range2188293
display id: range2188293
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 335
end: 337
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188289
display id: annotation2188289
version: 1
top level: CBDclos(T7
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188289/range2188289/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188289/range2188289/1
type: Range
persistent identity: range2188289
display id: range2188289
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 41
end: 316
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188284
display id: annotation2188284
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188284/range2188284/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188284/range2188284/1
type: Range
persistent identity: range2188284
display id: range2188284
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 21
end: 32
orientation: inline

T7
type: ComponentDefinition
persistent identity: BBa G00113
display id: BBa_G00113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter sequencing primer, 20-mer
creator: Reshma Shetty
created: 2008-10-16T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This primer anneals to the T7 Promoter region of any vector containing the T7 Promoter.
mutable notes: <biblio> #Wallace pmid=6282692 </biblio>
mutable provenance: T7 promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00113_sequence/1

BBa_K863113
type: ComponentDefinition
persistent identity: BBa K863113
display id: BBa_K863113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] with T7, RBS linked with two amino acids (GS) to a GFP with His-tag.
mutable notes: CBDclos fused to a his-tagged GFP in a Freiburg-manner
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_K525998
sequence annotation: GS-Linker
sequence annotation: Silent mutation (A original)
sequence annotation: T7 promoter
sequence annotation: ATG
sequence annotation: Cellulose Binding Protein
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: BBa_E0040
sequence annotation: AgeI
sequence annotation: BBa_K863111
sequence annotation: BBa_K863112
sequence annotation: B0034
sequence annotation: His-Tag
sequence annotation: RBS
sequence annotation: Freiburg-Scar
sequence annotation: BBa_K863121
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863113_sequence/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188387
display id: annotation2188387
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1
type: Range
persistent identity: range2188387
display id: range2188387
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1
end: 32
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188396
display id: annotation2188396
version: 1
top level: BBa_K863113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1
type: Range
persistent identity: range2188396
display id: range2188396
version: 1
direction: direction/forward
top level: BBa_K863113
start: 323
end: 328
orientation: inline

Silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200501
display id: annotation2200501
version: 1
top level: BBa_K863113
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1
type: Range
persistent identity: range2200501
display id: range2200501
version: 1
direction: direction/forward
top level: BBa_K863113
start: 112
end: 112
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188388
display id: annotation2188388
version: 1
top level: BBa_K863113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1
type: Range
persistent identity: range2188388
display id: range2188388
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1
end: 20
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188391
display id: annotation2188391
version: 1
top level: BBa_K863113
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1
type: Range
persistent identity: range2188391
display id: range2188391
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 38
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188392
display id: annotation2188392
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1
type: Range
persistent identity: range2188392
display id: range2188392
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 40
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188394
display id: annotation2188394
version: 1
top level: BBa_K863113
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1
type: Range
persistent identity: range2188394
display id: range2188394
version: 1
direction: direction/forward
top level: BBa_K863113
start: 41
end: 316
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188395
display id: annotation2188395
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1
type: Range
persistent identity: range2188395
display id: range2188395
version: 1
direction: direction/forward
top level: BBa_K863113
start: 317
end: 322
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188399
display id: annotation2188399
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1
type: Range
persistent identity: range2188399
display id: range2188399
version: 1
direction: direction/forward
top level: BBa_K863113
start: 335
end: 1048
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188401
display id: annotation2188401
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1
type: Range
persistent identity: range2188401
display id: range2188401
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1068
end: 1072
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188393
display id: annotation2188393
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1
type: Range
persistent identity: range2188393
display id: range2188393
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 328
orientation: inline

BBa_K863112
type: SequenceAnnotation
persistent identity: annotation2188386
display id: annotation2188386
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1
type: Range
persistent identity: range2188386
display id: range2188386
version: 1
direction: direction/reverse
top level: BBa_K863113
start: 1
end: 328
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188389
display id: annotation2188389
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1
type: Range
persistent identity: range2188389
display id: range2188389
version: 1
direction: direction/forward
top level: BBa_K863113
start: 21
end: 32
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188400
display id: annotation2188400
version: 1
top level: BBa_K863113
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1
type: Range
persistent identity: range2188400
display id: range2188400
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1049
end: 1066
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188390
display id: annotation2188390
version: 1
top level: BBa_K863113
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1
type: Range
persistent identity: range2188390
display id: range2188390
version: 1
direction: direction/forward
top level: BBa_K863113
start: 24
end: 27
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188397
display id: annotation2188397
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1
type: Range
persistent identity: range2188397
display id: range2188397
version: 1
direction: direction/forward
top level: BBa_K863113
start: 329
end: 334
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188398
display id: annotation2188398
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1
type: Range
persistent identity: range2188398
display id: range2188398
version: 1
direction: direction/reverse
top level: BBa_K863113
start: 335
end: 1075
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188402
display id: annotation2188402
version: 1
top level: BBa_K863113
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1
type: Range
persistent identity: range2188402
display id: range2188402
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1073
end: 1075
orientation: inline

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863103
display id: BBa_K863103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:19:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of C. Fimi Exoglucanase gene with T7, RBS linked with two amino acids (GS) to a GFP (Freiburg-Standard)
mutable notes: CBDcex fused to a his-tagged GFP in a Freiburg-manner
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: AgeI
sequence annotation: GS-Linker
sequence annotation: Exoglucanase
sequence annotation: RBS
sequence annotation: BBa_K525998
sequence annotation: BBa_K863101
sequence annotation: ATG
sequence annotation: B0034
sequence annotation: TAA
sequence annotation: T7 promoter
sequence annotation: BBa_K863102
sequence annotation: His-Tag
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: BBa_E0040
sequence annotation: Freiburg-Scar
sequence annotation: BBa_K863121
sequence: https://synbiohub.org/public/igem/BBa_K863103_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation2188027
display id: annotation2188027
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1
type: Range
persistent identity: range2188027
display id: range2188027
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1103
end: 1108
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188022
display id: annotation2188022
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1
type: Range
persistent identity: range2188022
display id: range2188022
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 359
end: 364
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188021
display id: annotation2188021
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1
type: Range
persistent identity: range2188021
display id: range2188021
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 350
end: 358
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188016
display id: annotation2188016
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1
type: Range
persistent identity: range2188016
display id: range2188016
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 24
end: 27
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188013
display id: annotation2188013
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1
type: Range
persistent identity: range2188013
display id: range2188013
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 32
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188018
display id: annotation2188018
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1
type: Range
persistent identity: range2188018
display id: range2188018
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 49
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2188019
display id: annotation2188019
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1
type: Range
persistent identity: range2188019
display id: range2188019
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 364
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188017
display id: annotation2188017
version: 1
top level: CBDcex(T7)
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1
type: Range
persistent identity: range2188017
display id: range2188017
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 35
end: 37
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188015
display id: annotation2188015
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1
type: Range
persistent identity: range2188015
display id: range2188015
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 21
end: 32
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188028
display id: annotation2188028
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1
type: Range
persistent identity: range2188028
display id: range2188028
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1109
end: 1111
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188012
display id: annotation2188012
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1
type: Range
persistent identity: range2188012
display id: range2188012
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 20
orientation: inline

BBa_K863102
type: SequenceAnnotation
persistent identity: annotation2188014
display id: annotation2188014
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1
type: Range
persistent identity: range2188014
display id: range2188014
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 364
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188026
display id: annotation2188026
version: 1
top level: CBDcex(T7)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1
type: Range
persistent identity: range2188026
display id: range2188026
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1085
end: 1102
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2188020
display id: annotation2188020
version: 1
top level: CBDcex(T7)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1
type: Range
persistent identity: range2188020
display id: range2188020
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 50
end: 349
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188024
display id: annotation2188024
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1
type: Range
persistent identity: range2188024
display id: range2188024
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 1084
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188023
display id: annotation2188023
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1
type: Range
persistent identity: range2188023
display id: range2188023
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 365
end: 370
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188025
display id: annotation2188025
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1
type: Range
persistent identity: range2188025
display id: range2188025
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 1111
orientation: inline

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863102
display id: BBa_K863102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-18T11:00:00Z
modified: 2015-06-17T01:19:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding domain of the [http://www.ncbi.nlm.nih.gov/nucleotide/327179207?report=genbank&log$=nucltop&blast_rank=3&RID=152ZCN0E01N Cellulomonas fimi ATCC 484 exoglucanase gene] in Freiburg-standard with T7, RBS, GS-Linker (Freiburg-Standard)
mutable notes: You can find additional info to the Cellulose binding domain here: [http://partsregistry.org/Part:BBa_K863101:Design CBDcex_Freiburg]
mutable provenance: Cloning-vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Exoglucanase
sequence annotation: T7 promoter
sequence annotation: TAA
sequence annotation: BBa_K525998
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: GS-Linker
sequence annotation: RBS
sequence annotation: BBa_K863101
sequence annotation: ATG
sequence annotation: B0034
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K863102_sequence/1

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2187508
display id: annotation2187508
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187508/range2187508/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187508/range2187508/1
type: Range
persistent identity: range2187508
display id: range2187508
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 49
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2187503
display id: annotation2187503
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187503/range2187503/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187503/range2187503/1
type: Range
persistent identity: range2187503
display id: range2187503
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 20
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2187514
display id: annotation2187514
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187514/range2187514/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187514/range2187514/1
type: Range
persistent identity: range2187514
display id: range2187514
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 373
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2187504
display id: annotation2187504
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187504/range2187504/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187504/range2187504/1
type: Range
persistent identity: range2187504
display id: range2187504
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 32
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2187510
display id: annotation2187510
version: 1
top level: CBDcex(T7)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187510/range2187510/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187510/range2187510/1
type: Range
persistent identity: range2187510
display id: range2187510
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 50
end: 349
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2187512
display id: annotation2187512
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187512/range2187512/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187512/range2187512/1
type: Range
persistent identity: range2187512
display id: range2187512
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 359
end: 364
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2187511
display id: annotation2187511
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187511/range2187511/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187511/range2187511/1
type: Range
persistent identity: range2187511
display id: range2187511
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 350
end: 358
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2187506
display id: annotation2187506
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187506/range2187506/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187506/range2187506/1
type: Range
persistent identity: range2187506
display id: range2187506
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 24
end: 27
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2187509
display id: annotation2187509
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187509/range2187509/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187509/range2187509/1
type: Range
persistent identity: range2187509
display id: range2187509
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 373
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2187507
display id: annotation2187507
version: 1
top level: CBDcex(T7)
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187507/range2187507/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187507/range2187507/1
type: Range
persistent identity: range2187507
display id: range2187507
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 35
end: 37
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2187505
display id: annotation2187505
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187505/range2187505/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187505/range2187505/1
type: Range
persistent identity: range2187505
display id: range2187505
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 21
end: 32
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2187513
display id: annotation2187513
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187513/range2187513/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187513/range2187513/1
type: Range
persistent identity: range2187513
display id: range2187513
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 365
end: 370
orientation: inline