//promoter/anderson
type: Collection
persistent identity: promoter anderson
display id: promoter_anderson
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K777128
member: BBa_J23100
member: BBa_K777107
member: BBa_K777123
member: BBa_K823006
member: BBa_J23105
member: BBa_K1585103
member: BBa_J23114
member: BBa_K777002
member: BBa_K777116
member: BBa_K823013
member: BBa_K1585110
member: BBa_K777004
member: BBa_J23112
member: BBa_J23119
member: BBa_K1468000
member: BBa_K1585101
member: BBa_K1585117
member: BBa_K777125
member: BBa_K823004
member: BBa_J23102
member: BBa_K2122100
member: BBa_K777104
member: BBa_K777130
member: CP1LacPI2
member: BBa_K777126
member: BBa_J23107
member: BBa_J23108
member: BBa_K2122000
member: BBa_K777006
member: BBa_K777000
member: BBa_K777120
member: BBa_K823011
member: BBa_K1468002
member: BBa_K777114
member: BBa_J23101
member: BBa_K777127
member: BBa_K1330002
member: CP1LacPI1
member: BBa_K777007
member: BBa_J23109
member: BBa_K1585115
member: BBa_J23113
member: BBa_K1679004
member: BBa_K1824896
member: BBa_K777102
member: BBa_K777132
member: BBa_K823010
member: BBa_K777115
member: BBa_J23118
member: BBa_K1468001
member: BBa_K823014
member: BBa_K777008
member: BBa_K1585102
member: BBa_K777110
member: BBa_K823005
member: BBa_K1585116
member: BBa_K777101
member: BBa_K777131
member: BBa_K777108
member: BBa_K777122
member: BBa_K823007
member: BBa_K777129
member: BBa_J23106
member: BBa_K1585104
member: CP2LacPI1
member: BBa_J23115
member: BBa_K777001
member: BBa_K777005
member: BBa_J23111
member: BBa_K256002
member: BBa_K1585100

BBa_K823005
type: ComponentDefinition
persistent identity: BBa K823005
display id: BBa_K823005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23101
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:44:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23101 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: J23101
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823005_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182510
display id: annotation2182510
version: 1
top level: BBa_K823005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1
type: Range
persistent identity: range2182510
display id: range2182510
version: 1
direction: direction/forward
top level: BBa_K823005
start: 1
end: 6
orientation: inline

J23101
type: SequenceAnnotation
persistent identity: annotation2182509
display id: annotation2182509
version: 1
top level: BBa_K823005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1
type: Range
persistent identity: range2182509
display id: range2182509
version: 1
direction: direction/forward
top level: BBa_K823005
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182511
display id: annotation2182511
version: 1
top level: BBa_K823005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1
type: Range
persistent identity: range2182511
display id: range2182511
version: 1
direction: direction/forward
top level: BBa_K823005
start: 24
end: 29
orientation: inline

BBa_J23115
type: ComponentDefinition
persistent identity: BBa J23115
display id: BBa_J23115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23115_sequence/1

BBa_K777118
type: ComponentDefinition
persistent identity: BBa K777118
display id: BBa_K777118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
mutable notes: * ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777117
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K777117
sequence: https://synbiohub.org/public/igem/BBa_K777118_sequence/1

BBa_K777117
type: Component
persistent identity: component2190664
display id: component2190664
version: 1
top level: BBa_K777118
access: public
definition: https://synbiohub.org/public/igem/BBa_K777117/1

BBa_J23100
type: Component
persistent identity: component2190662
display id: component2190662
version: 1
top level: BBa_K777118
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190662
display id: annotation2190662
version: 1
top level: BBa_K777118
location: https://synbiohub.org/public/igem/BBa_K777118/annotation2190662/range2190662/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777118/annotation2190662/range2190662/1
type: Range
persistent identity: range2190662
display id: range2190662
version: 1
direction: direction/reverse
top level: BBa_K777118
start: 1
end: 35
orientation: inline

BBa_K777117
type: SequenceAnnotation
persistent identity: annotation2190664
display id: annotation2190664
version: 1
top level: BBa_K777118
location: https://synbiohub.org/public/igem/BBa_K777118/annotation2190664/range2190664/1
component: BBa_K777117

https://synbiohub.org/public/igem/BBa_K777118/annotation2190664/range2190664/1
type: Range
persistent identity: range2190664
display id: range2190664
version: 1
direction: direction/reverse
top level: BBa_K777118
start: 42
end: 968
orientation: inline

BBa_K292000
type: ComponentDefinition
persistent identity: BBa K292000
display id: BBa_K292000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double terminator + constitutive promoter
creator: David Charoy
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: true
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a double terminator and a constitutive prokaryotic promoter. It is a simple promoter to begin gene transcription. For Sup???biotech-Paris (2009) team this part is used after to be added to a recombinant lambda phage to begin the transcription of synthetic genes inside the Lambda phage genome.
mutable notes: N/A
mutable provenance: BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16) BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23100
component: BBa_B0012
component: BBa_B0011
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K292000_sequence/1

BBa_J23100
type: Component
persistent identity: component2059163
display id: component2059163
version: 1
top level: BBa_K292000
access: public
definition: BBa_J23100

BBa_B0012
type: Component
persistent identity: component2059157
display id: component2059157
version: 1
top level: BBa_K292000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component2059161
display id: component2059161
version: 1
top level: BBa_K292000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2059161
display id: annotation2059161
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059161/range2059161/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292000/annotation2059161/range2059161/1
type: Range
persistent identity: range2059161
display id: range2059161
version: 1
direction: direction/forward
top level: BBa_K292000
start: 50
end: 95
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2059157
display id: annotation2059157
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059157/range2059157/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292000/annotation2059157/range2059157/1
type: Range
persistent identity: range2059157
display id: range2059157
version: 1
direction: direction/forward
top level: BBa_K292000
start: 1
end: 41
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2059163
display id: annotation2059163
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059163/range2059163/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K292000/annotation2059163/range2059163/1
type: Range
persistent identity: range2059163
display id: range2059163
version: 1
direction: direction/reverse
top level: BBa_K292000
start: 104
end: 138
orientation: inline

BBa_K1468000
type: ComponentDefinition
persistent identity: BBa K1468000
display id: BBa_K1468000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0040
sequence annotation: BBa_J23104
sequence annotation: STOP
sequence annotation: ZsGreeen
sequence annotation: BBa_K1468000
sequence annotation: ZsGreen protein
sequence annotation: START
sequence annotation: J23104p
sequence: https://synbiohub.org/public/igem/BBa_K1468000_sequence/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2418060
display id: annotation2418060
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1
type: Range
persistent identity: range2418060
display id: range2418060
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2418058
display id: annotation2418058
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1
type: Range
persistent identity: range2418058
display id: range2418058
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418064
display id: annotation2418064
version: 1
top level: BBa_K1468000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1
type: Range
persistent identity: range2418064
display id: range2418064
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 725
end: 725
orientation: inline

ZsGreeen
type: SequenceAnnotation
persistent identity: annotation2418062
display id: annotation2418062
version: 1
top level: BBa_K1468000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1
type: Range
persistent identity: range2418062
display id: range2418062
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

BBa_K1468000
type: SequenceAnnotation
persistent identity: annotation2418810
display id: annotation2418810
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1
type: Range
persistent identity: range2418810
display id: range2418810
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 725
orientation: inline

ZsGreen protein
type: SequenceAnnotation
persistent identity: annotation2418059
display id: annotation2418059
version: 1
top level: BBa_K1468000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1
type: Range
persistent identity: range2418059
display id: range2418059
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2418061
display id: annotation2418061
version: 1
top level: BBa_K1468000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1
type: Range
persistent identity: range2418061
display id: range2418061
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 36
orientation: inline

J23104p
type: SequenceAnnotation
persistent identity: annotation2418030
display id: annotation2418030
version: 1
top level: BBa_K1468000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1
type: Range
persistent identity: range2418030
display id: range2418030
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_K777108
type: ComponentDefinition
persistent identity: BBa K777108
display id: BBa_K777108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23114
sequence annotation: BBa_J23114
sequence annotation: BBa_K777100
sequence: https://synbiohub.org/public/igem/BBa_K777108_sequence/1

BBa_K777100
type: Component
persistent identity: component2187126
display id: component2187126
version: 1
top level: BBa_K777108
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23114
type: Component
persistent identity: component2187118
display id: component2187118
version: 1
top level: BBa_K777108
access: public
definition: BBa_J23114

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2187118
display id: annotation2187118
version: 1
top level: BBa_K777108
location: https://synbiohub.org/public/igem/BBa_K777108/annotation2187118/range2187118/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777108/annotation2187118/range2187118/1
type: Range
persistent identity: range2187118
display id: range2187118
version: 1
direction: direction/reverse
top level: BBa_K777108
start: 1
end: 35
orientation: inline

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187126
display id: annotation2187126
version: 1
top level: BBa_K777108
location: https://synbiohub.org/public/igem/BBa_K777108/annotation2187126/range2187126/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777108/annotation2187126/range2187126/1
type: Range
persistent identity: range2187126
display id: range2187126
version: 1
direction: direction/reverse
top level: BBa_K777108
start: 42
end: 910
orientation: inline

BBa_K777111
type: ComponentDefinition
persistent identity: BBa K777111
display id: BBa_K777111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliC under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. coli''. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''fliC'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: * The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
mutable provenance: * The ''fliC'' gene was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23112
component: BBa_K777109
sequence annotation: BBa_K777109
sequence annotation: BBa_J23112
sequence: https://synbiohub.org/public/igem/BBa_K777111_sequence/1

BBa_J23112
type: Component
persistent identity: component2192720
display id: component2192720
version: 1
top level: BBa_K777111
access: public
definition: BBa_J23112

BBa_K777109
type: Component
persistent identity: component2192728
display id: component2192728
version: 1
top level: BBa_K777111
access: public
definition: https://synbiohub.org/public/igem/BBa_K777109/1

BBa_K777109
type: SequenceAnnotation
persistent identity: annotation2192728
display id: annotation2192728
version: 1
top level: BBa_K777111
location: https://synbiohub.org/public/igem/BBa_K777111/annotation2192728/range2192728/1
component: BBa_K777109

https://synbiohub.org/public/igem/BBa_K777111/annotation2192728/range2192728/1
type: Range
persistent identity: range2192728
display id: range2192728
version: 1
direction: direction/reverse
top level: BBa_K777111
start: 42
end: 1538
orientation: inline

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2192720
display id: annotation2192720
version: 1
top level: BBa_K777111
location: https://synbiohub.org/public/igem/BBa_K777111/annotation2192720/range2192720/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777111/annotation2192720/range2192720/1
type: Range
persistent identity: range2192720
display id: range2192720
version: 1
direction: direction/reverse
top level: BBa_K777111
start: 1
end: 35
orientation: inline

BBa_J23108
type: ComponentDefinition
persistent identity: BBa J23108
display id: BBa_J23108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23108_sequence/1

BBa_K1585117
type: ComponentDefinition
persistent identity: BBa K1585117
display id: BBa_K1585117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:23:31Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23116 J23116] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23117
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23117
sequence: https://synbiohub.org/public/igem/BBa_K1585117_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441713
display id: component2441713
version: 1
top level: BBa_K1585117
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23117
type: Component
persistent identity: component2441711
display id: component2441711
version: 1
top level: BBa_K1585117
access: public
definition: BBa_J23117

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441713
display id: annotation2441713
version: 1
top level: BBa_K1585117
location: https://synbiohub.org/public/igem/BBa_K1585117/annotation2441713/range2441713/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585117/annotation2441713/range2441713/1
type: Range
persistent identity: range2441713
display id: range2441713
version: 1
direction: direction/reverse
top level: BBa_K1585117
start: 44
end: 78
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation2441711
display id: annotation2441711
version: 1
top level: BBa_K1585117
location: https://synbiohub.org/public/igem/BBa_K1585117/annotation2441711/range2441711/1
component: BBa_J23117

https://synbiohub.org/public/igem/BBa_K1585117/annotation2441711/range2441711/1
type: Range
persistent identity: range2441711
display id: range2441711
version: 1
direction: direction/reverse
top level: BBa_K1585117
start: 1
end: 35
orientation: inline

BBa_K1585115
type: ComponentDefinition
persistent identity: BBa K1585115
display id: BBa_K1585115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:13:08Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23110 J23110] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23115
component: BBa_K1585999
sequence annotation: BBa_J23115
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585115_sequence/1

BBa_J23115
type: Component
persistent identity: component2441705
display id: component2441705
version: 1
top level: BBa_K1585115
access: public
definition: BBa_J23115

BBa_K1585999
type: Component
persistent identity: component2441707
display id: component2441707
version: 1
top level: BBa_K1585115
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2441705
display id: annotation2441705
version: 1
top level: BBa_K1585115
location: https://synbiohub.org/public/igem/BBa_K1585115/annotation2441705/range2441705/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_K1585115/annotation2441705/range2441705/1
type: Range
persistent identity: range2441705
display id: range2441705
version: 1
direction: direction/reverse
top level: BBa_K1585115
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441707
display id: annotation2441707
version: 1
top level: BBa_K1585115
location: https://synbiohub.org/public/igem/BBa_K1585115/annotation2441707/range2441707/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585115/annotation2441707/range2441707/1
type: Range
persistent identity: range2441707
display id: range2441707
version: 1
direction: direction/reverse
top level: BBa_K1585115
start: 44
end: 78
orientation: inline

BBa_K777004
type: ComponentDefinition
persistent identity: BBa K777004
display id: BBa_K777004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23106
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23106 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23106 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777000
component: BBa_J23106
sequence annotation: BBa_K777000
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K777004_sequence/1

BBa_K777000
type: Component
persistent identity: component2189302
display id: component2189302
version: 1
top level: BBa_K777004
access: public
definition: BBa_K777000

BBa_J23106
type: Component
persistent identity: component2189298
display id: component2189298
version: 1
top level: BBa_K777004
access: public
definition: BBa_J23106

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189302
display id: annotation2189302
version: 1
top level: BBa_K777004
location: https://synbiohub.org/public/igem/BBa_K777004/annotation2189302/range2189302/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777004/annotation2189302/range2189302/1
type: Range
persistent identity: range2189302
display id: range2189302
version: 1
direction: direction/reverse
top level: BBa_K777004
start: 42
end: 1703
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2189298
display id: annotation2189298
version: 1
top level: BBa_K777004
location: https://synbiohub.org/public/igem/BBa_K777004/annotation2189298/range2189298/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K777004/annotation2189298/range2189298/1
type: Range
persistent identity: range2189298
display id: range2189298
version: 1
direction: direction/reverse
top level: BBa_K777004
start: 1
end: 35
orientation: inline

CP1LacPI1
type: ComponentDefinition
persistent identity: BBa K418000
display id: BBa_K418000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: true
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6657
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constituitive promoter + LacPI
mutable notes: blah
mutable provenance: From assembly of kit parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_Q01121
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_Q01121
sequence: https://synbiohub.org/public/igem/BBa_K418000_sequence/1

BBa_Q01121
type: Component
persistent identity: component2263742
display id: component2263742
version: 1
top level: CP1LacPI1
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_J23100
type: Component
persistent identity: component2263723
display id: component2263723
version: 1
top level: CP1LacPI1
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2263723
display id: annotation2263723
version: 1
top level: CP1LacPI1
location: https://synbiohub.org/public/igem/BBa_K418000/annotation2263723/range2263723/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K418000/annotation2263723/range2263723/1
type: Range
persistent identity: range2263723
display id: range2263723
version: 1
direction: direction/reverse
top level: CP1LacPI1
start: 1
end: 35
orientation: inline

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2263742
display id: annotation2263742
version: 1
top level: CP1LacPI1
location: https://synbiohub.org/public/igem/BBa_K418000/annotation2263742/range2263742/1
component: BBa_Q01121

https://synbiohub.org/public/igem/BBa_K418000/annotation2263742/range2263742/1
type: Range
persistent identity: range2263742
display id: range2263742
version: 1
direction: direction/reverse
top level: CP1LacPI1
start: 44
end: 1416
orientation: inline

BBa_K823004
type: ComponentDefinition
persistent identity: BBa K823004
display id: BBa_K823004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23100
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:43:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23100 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23100
sequence annotation: -35 box
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823004_sequence/1

J23100
type: SequenceAnnotation
persistent identity: annotation2182506
display id: annotation2182506
version: 1
top level: BBa_K823004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182506/range2182506/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182506/range2182506/1
type: Range
persistent identity: range2182506
display id: range2182506
version: 1
direction: direction/forward
top level: BBa_K823004
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182507
display id: annotation2182507
version: 1
top level: BBa_K823004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182507/range2182507/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182507/range2182507/1
type: Range
persistent identity: range2182507
display id: range2182507
version: 1
direction: direction/forward
top level: BBa_K823004
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182508
display id: annotation2182508
version: 1
top level: BBa_K823004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182508/range2182508/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182508/range2182508/1
type: Range
persistent identity: range2182508
display id: range2182508
version: 1
direction: direction/forward
top level: BBa_K823004
start: 24
end: 29
orientation: inline

BBa_K777000
type: ComponentDefinition
persistent identity: BBa K777000
display id: BBa_K777000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor
creator: Bianca Genenncher
created: 2012-09-10T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: The transmembrane receptor Tar of E. coli mediates chemotaxis towards aspartate.
mutable notes: One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
mutable provenance: The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B chromosome (NC_010473.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Tar coding region
sequence annotation: TGA
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K777000_sequence/1

Tar coding region
type: SequenceAnnotation
persistent identity: annotation2182660
display id: annotation2182660
version: 1
top level: BBa_K777000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777000/annotation2182660/range2182660/1

https://synbiohub.org/public/igem/BBa_K777000/annotation2182660/range2182660/1
type: Range
persistent identity: range2182660
display id: range2182660
version: 1
direction: direction/forward
top level: BBa_K777000
start: 1
end: 1662
orientation: inline

TGA
type: SequenceAnnotation
persistent identity: annotation2182661
display id: annotation2182661
version: 1
top level: BBa_K777000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777000/annotation2182661/range2182661/1

https://synbiohub.org/public/igem/BBa_K777000/annotation2182661/range2182661/1
type: Range
persistent identity: range2182661
display id: range2182661
version: 1
direction: direction/forward
top level: BBa_K777000
start: 1660
end: 1662
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2182659
display id: annotation2182659
version: 1
top level: BBa_K777000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777000/annotation2182659/range2182659/1

https://synbiohub.org/public/igem/BBa_K777000/annotation2182659/range2182659/1
type: Range
persistent identity: range2182659
display id: range2182659
version: 1
direction: direction/forward
top level: BBa_K777000
start: 1
end: 3
orientation: inline

BBa_J23107
type: ComponentDefinition
persistent identity: BBa J23107
display id: BBa_J23107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23107_sequence/1

BBa_K777112
type: ComponentDefinition
persistent identity: BBa K777112
display id: BBa_K777112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliC under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. coli''. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''fliC'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: * The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
mutable provenance: * The ''fliC'' gene was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777109
component: BBa_J23114
sequence annotation: BBa_K777109
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K777112_sequence/1

BBa_K777109
type: Component
persistent identity: component2192754
display id: component2192754
version: 1
top level: BBa_K777112
access: public
definition: https://synbiohub.org/public/igem/BBa_K777109/1

BBa_J23114
type: Component
persistent identity: component2192746
display id: component2192746
version: 1
top level: BBa_K777112
access: public
definition: BBa_J23114

BBa_K777109
type: SequenceAnnotation
persistent identity: annotation2192754
display id: annotation2192754
version: 1
top level: BBa_K777112
location: https://synbiohub.org/public/igem/BBa_K777112/annotation2192754/range2192754/1
component: BBa_K777109

https://synbiohub.org/public/igem/BBa_K777112/annotation2192754/range2192754/1
type: Range
persistent identity: range2192754
display id: range2192754
version: 1
direction: direction/reverse
top level: BBa_K777112
start: 42
end: 1538
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2192746
display id: annotation2192746
version: 1
top level: BBa_K777112
location: https://synbiohub.org/public/igem/BBa_K777112/annotation2192746/range2192746/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777112/annotation2192746/range2192746/1
type: Range
persistent identity: range2192746
display id: range2192746
version: 1
direction: direction/reverse
top level: BBa_K777112
start: 1
end: 35
orientation: inline

BBa_K823011
type: ComponentDefinition
persistent identity: BBa K823011
display id: BBa_K823011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23114
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:50:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23114 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23114
sequence annotation: -35 box
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823011_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2182537
display id: annotation2182537
version: 1
top level: BBa_K823011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182537/range2182537/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182537/range2182537/1
type: Range
persistent identity: range2182537
display id: range2182537
version: 1
direction: direction/forward
top level: BBa_K823011
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182538
display id: annotation2182538
version: 1
top level: BBa_K823011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182538/range2182538/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182538/range2182538/1
type: Range
persistent identity: range2182538
display id: range2182538
version: 1
direction: direction/forward
top level: BBa_K823011
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182539
display id: annotation2182539
version: 1
top level: BBa_K823011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182539/range2182539/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182539/range2182539/1
type: Range
persistent identity: range2182539
display id: range2182539
version: 1
direction: direction/forward
top level: BBa_K823011
start: 24
end: 29
orientation: inline

BBa_K777104
type: ComponentDefinition
persistent identity: BBa K777104
display id: BBa_K777104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23106
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: * The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer * ''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' ** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23106 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23106
sequence annotation: BBa_K777100
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K777104_sequence/1

BBa_K777100
type: Component
persistent identity: component2187047
display id: component2187047
version: 1
top level: BBa_K777104
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23106
type: Component
persistent identity: component2187039
display id: component2187039
version: 1
top level: BBa_K777104
access: public
definition: BBa_J23106

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187047
display id: annotation2187047
version: 1
top level: BBa_K777104
location: https://synbiohub.org/public/igem/BBa_K777104/annotation2187047/range2187047/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777104/annotation2187047/range2187047/1
type: Range
persistent identity: range2187047
display id: range2187047
version: 1
direction: direction/reverse
top level: BBa_K777104
start: 42
end: 910
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2187039
display id: annotation2187039
version: 1
top level: BBa_K777104
location: https://synbiohub.org/public/igem/BBa_K777104/annotation2187039/range2187039/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K777104/annotation2187039/range2187039/1
type: Range
persistent identity: range2187039
display id: range2187039
version: 1
direction: direction/reverse
top level: BBa_K777104
start: 1
end: 35
orientation: inline

BBa_K777132
type: ComponentDefinition
persistent identity: BBa K777132
display id: BBa_K777132
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive J23114 promoter
creator: Team Goettingen
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23114 J23114]) and the plasmid backbone pSB1C3.
mutable notes: * No specific design considerations
mutable provenance: * pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: J23114
sequence annotation: constitutive promoter J23114
sequence: https://synbiohub.org/public/igem/BBa_K777132_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2204948
display id: annotation2204948
version: 1
top level: BBa_K777132
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777132/annotation2204948/range2204948/1

https://synbiohub.org/public/igem/BBa_K777132/annotation2204948/range2204948/1
type: Range
persistent identity: range2204948
display id: range2204948
version: 1
direction: direction/forward
top level: BBa_K777132
start: 2
end: 36
orientation: inline

constitutive promoter J23114
type: SequenceAnnotation
persistent identity: annotation2204949
display id: annotation2204949
version: 1
top level: BBa_K777132
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K777132/annotation2204949/range2204949/1

https://synbiohub.org/public/igem/BBa_K777132/annotation2204949/range2204949/1
type: Range
persistent identity: range2204949
display id: range2204949
version: 1
direction: direction/forward
top level: BBa_K777132
start: 2
end: 36
orientation: inline

BBa_K1330002
type: ComponentDefinition
persistent identity: BBa K1330002
display id: BBa_K1330002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter (J23105)
creator: Kristian Jensen
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1705_
m user id: 0
owner id: 20126
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1330002_sequence/1

BBa_J23103
type: ComponentDefinition
persistent identity: BBa J23103
display id: BBa_J23103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23103_sequence/1

BBa_K1585101
type: ComponentDefinition
persistent identity: BBa K1585101
display id: BBa_K1585101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-23T11:00:00Z
modified: 2015-09-01T03:27:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same. We choosed part BBa_R0010 because we assumed older parts would be reliable.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23101
component: BBa_K1585999
sequence annotation: BBa_J23101
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585101_sequence/1

BBa_J23101
type: Component
persistent identity: component2436906
display id: component2436906
version: 1
top level: BBa_K1585101
access: public
definition: BBa_J23101

BBa_K1585999
type: Component
persistent identity: component2436907
display id: component2436907
version: 1
top level: BBa_K1585101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2436906
display id: annotation2436906
version: 1
top level: BBa_K1585101
location: https://synbiohub.org/public/igem/BBa_K1585101/annotation2436906/range2436906/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1585101/annotation2436906/range2436906/1
type: Range
persistent identity: range2436906
display id: range2436906
version: 1
direction: direction/reverse
top level: BBa_K1585101
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2436907
display id: annotation2436907
version: 1
top level: BBa_K1585101
location: https://synbiohub.org/public/igem/BBa_K1585101/annotation2436907/range2436907/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585101/annotation2436907/range2436907/1
type: Range
persistent identity: range2436907
display id: range2436907
version: 1
direction: direction/reverse
top level: BBa_K1585101
start: 44
end: 78
orientation: inline

BBa_K777102
type: ComponentDefinition
persistent identity: BBa K777102
display id: BBa_K777102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23104
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive flhDC expression affect the motility of E. coli.
mutable notes: * The PstI restriction site in the flhDC sequence was mutated to CTGCGG using the reverse primer * flhDC was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' ** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23104
sequence annotation: BBa_K777100
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K777102_sequence/1

BBa_K777100
type: Component
persistent identity: component2187014
display id: component2187014
version: 1
top level: BBa_K777102
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23104
type: Component
persistent identity: component2187006
display id: component2187006
version: 1
top level: BBa_K777102
access: public
definition: BBa_J23104

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187014
display id: annotation2187014
version: 1
top level: BBa_K777102
location: https://synbiohub.org/public/igem/BBa_K777102/annotation2187014/range2187014/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777102/annotation2187014/range2187014/1
type: Range
persistent identity: range2187014
display id: range2187014
version: 1
direction: direction/reverse
top level: BBa_K777102
start: 42
end: 910
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2187006
display id: annotation2187006
version: 1
top level: BBa_K777102
location: https://synbiohub.org/public/igem/BBa_K777102/annotation2187006/range2187006/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K777102/annotation2187006/range2187006/1
type: Range
persistent identity: range2187006
display id: range2187006
version: 1
direction: direction/reverse
top level: BBa_K777102
start: 1
end: 35
orientation: inline

BBa_K2122100
type: ComponentDefinition
persistent identity: BBa K2122100
display id: BBa_K2122100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Device expressing the Gb3 Synthase enzyme under control of a constitutive promoter
creator: Sannia Farrukh
created: 2016-09-28T11:00:00Z
modified: 2016-10-18T01:30:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2591_
m user id: 32074
owner id: 32074
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
mutable notes: A constitutive promoter was added to the sequence
mutable provenance: Coding Sequence from GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0014
component: BBa_B0034
component: BBa_J23100
component: BBa_K2122150
sequence annotation: BBa_B0014
sequence annotation: BBa_B0034
sequence annotation: BBa_K2122150
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K2122100_sequence/1

BBa_B0014
type: Component
persistent identity: component2518023
display id: component2518023
version: 1
top level: BBa_K2122100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0034
type: Component
persistent identity: component2518015
display id: component2518015
version: 1
top level: BBa_K2122100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23100
type: Component
persistent identity: component2518013
display id: component2518013
version: 1
top level: BBa_K2122100
access: public
definition: BBa_J23100

BBa_K2122150
type: Component
persistent identity: component2518016
display id: component2518016
version: 1
top level: BBa_K2122100
access: public
definition: https://synbiohub.org/public/igem/BBa_K2122150/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518023
display id: annotation2518023
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518023/range2518023/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518023/range2518023/1
type: Range
persistent identity: range2518023
display id: range2518023
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 1132
end: 1226
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518015
display id: annotation2518015
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518015/range2518015/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518015/range2518015/1
type: Range
persistent identity: range2518015
display id: range2518015
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 44
end: 55
orientation: inline

BBa_K2122150
type: SequenceAnnotation
persistent identity: annotation2518016
display id: annotation2518016
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518016/range2518016/1
component: BBa_K2122150

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518016/range2518016/1
type: Range
persistent identity: range2518016
display id: range2518016
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 62
end: 1123
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2518013
display id: annotation2518013
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518013/range2518013/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518013/range2518013/1
type: Range
persistent identity: range2518013
display id: range2518013
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 1
end: 35
orientation: inline

BBa_K823013
type: ComponentDefinition
persistent identity: BBa K823013
display id: BBa_K823013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23117
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:51:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23117 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: J23117
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823013_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182544
display id: annotation2182544
version: 1
top level: BBa_K823013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182544/range2182544/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182544/range2182544/1
type: Range
persistent identity: range2182544
display id: range2182544
version: 1
direction: direction/forward
top level: BBa_K823013
start: 1
end: 6
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2182543
display id: annotation2182543
version: 1
top level: BBa_K823013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182543/range2182543/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182543/range2182543/1
type: Range
persistent identity: range2182543
display id: range2182543
version: 1
direction: direction/forward
top level: BBa_K823013
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182545
display id: annotation2182545
version: 1
top level: BBa_K823013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182545/range2182545/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182545/range2182545/1
type: Range
persistent identity: range2182545
display id: range2182545
version: 1
direction: direction/forward
top level: BBa_K823013
start: 24
end: 29
orientation: inline

BBa_K1585105
type: ComponentDefinition
persistent identity: BBa K1585105
display id: BBa_K1585105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:03:04Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23105
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23105
sequence: https://synbiohub.org/public/igem/BBa_K1585105_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441416
display id: component2441416
version: 1
top level: BBa_K1585105
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23105
type: Component
persistent identity: component2441414
display id: component2441414
version: 1
top level: BBa_K1585105
access: public
definition: BBa_J23105

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441416
display id: annotation2441416
version: 1
top level: BBa_K1585105
location: https://synbiohub.org/public/igem/BBa_K1585105/annotation2441416/range2441416/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585105/annotation2441416/range2441416/1
type: Range
persistent identity: range2441416
display id: range2441416
version: 1
direction: direction/reverse
top level: BBa_K1585105
start: 44
end: 78
orientation: inline

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2441414
display id: annotation2441414
version: 1
top level: BBa_K1585105
location: https://synbiohub.org/public/igem/BBa_K1585105/annotation2441414/range2441414/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K1585105/annotation2441414/range2441414/1
type: Range
persistent identity: range2441414
display id: range2441414
version: 1
direction: direction/reverse
top level: BBa_K1585105
start: 1
end: 35
orientation: inline

BBa_J23105
type: ComponentDefinition
persistent identity: BBa J23105
display id: BBa_J23105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23105_sequence/1

BBa_K256002
type: ComponentDefinition
persistent identity: BBa K256002
display id: BBa_K256002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101:GFP
creator: iGEM09_NTU-Singapore
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: GFP Reporter
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23101
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K256002_sequence/1

BBa_J23101
type: Component
persistent identity: component2056715
display id: component2056715
version: 1
top level: BBa_K256002
access: public
definition: BBa_J23101

BBa_B0012
type: Component
persistent identity: component2056722
display id: component2056722
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2056720
display id: component2056720
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2056717
display id: component2056717
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2056719
display id: component2056719
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056722
display id: annotation2056722
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056722/range2056722/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256002/annotation2056722/range2056722/1
type: Range
persistent identity: range2056722
display id: range2056722
version: 1
direction: direction/forward
top level: BBa_K256002
start: 878
end: 918
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2056719
display id: annotation2056719
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056719/range2056719/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K256002/annotation2056719/range2056719/1
type: Range
persistent identity: range2056719
display id: range2056719
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 62
end: 781
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056720
display id: annotation2056720
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056720/range2056720/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256002/annotation2056720/range2056720/1
type: Range
persistent identity: range2056720
display id: range2056720
version: 1
direction: direction/forward
top level: BBa_K256002
start: 790
end: 869
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056717
display id: annotation2056717
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056717/range2056717/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256002/annotation2056717/range2056717/1
type: Range
persistent identity: range2056717
display id: range2056717
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 44
end: 55
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2056715
display id: annotation2056715
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056715/range2056715/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K256002/annotation2056715/range2056715/1
type: Range
persistent identity: range2056715
display id: range2056715
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 1
end: 35
orientation: inline

BBa_K1585106
type: ComponentDefinition
persistent identity: BBa K1585106
display id: BBa_K1585106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:01:39Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23106 J23106] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23106
sequence annotation: BBa_J23106
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585106_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441698
display id: component2441698
version: 1
top level: BBa_K1585106
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23106
type: Component
persistent identity: component2441696
display id: component2441696
version: 1
top level: BBa_K1585106
access: public
definition: BBa_J23106

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2441696
display id: annotation2441696
version: 1
top level: BBa_K1585106
location: https://synbiohub.org/public/igem/BBa_K1585106/annotation2441696/range2441696/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1585106/annotation2441696/range2441696/1
type: Range
persistent identity: range2441696
display id: range2441696
version: 1
direction: direction/reverse
top level: BBa_K1585106
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441698
display id: annotation2441698
version: 1
top level: BBa_K1585106
location: https://synbiohub.org/public/igem/BBa_K1585106/annotation2441698/range2441698/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585106/annotation2441698/range2441698/1
type: Range
persistent identity: range2441698
display id: range2441698
version: 1
direction: direction/reverse
top level: BBa_K1585106
start: 44
end: 78
orientation: inline

BBa_K777116
type: ComponentDefinition
persistent identity: BBa K777116
display id: BBa_K777116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
mutable provenance: * ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23114 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23114
component: BBa_K777113
sequence annotation: BBa_K777113
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K777116_sequence/1

BBa_J23114
type: Component
persistent identity: component2189732
display id: component2189732
version: 1
top level: BBa_K777116
access: public
definition: BBa_J23114

BBa_K777113
type: Component
persistent identity: component2189734
display id: component2189734
version: 1
top level: BBa_K777116
access: public
definition: https://synbiohub.org/public/igem/BBa_K777113/1

BBa_K777113
type: SequenceAnnotation
persistent identity: annotation2189734
display id: annotation2189734
version: 1
top level: BBa_K777116
location: https://synbiohub.org/public/igem/BBa_K777116/annotation2189734/range2189734/1
component: BBa_K777113

https://synbiohub.org/public/igem/BBa_K777116/annotation2189734/range2189734/1
type: Range
persistent identity: range2189734
display id: range2189734
version: 1
direction: direction/reverse
top level: BBa_K777116
start: 42
end: 872
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2189732
display id: annotation2189732
version: 1
top level: BBa_K777116
location: https://synbiohub.org/public/igem/BBa_K777116/annotation2189732/range2189732/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777116/annotation2189732/range2189732/1
type: Range
persistent identity: range2189732
display id: range2189732
version: 1
direction: direction/reverse
top level: BBa_K777116
start: 1
end: 35
orientation: inline

BBa_K777123
type: ComponentDefinition
persistent identity: BBa K777123
display id: BBa_K777123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
mutable provenance: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23112
component: BBa_K777121
sequence annotation: BBa_J23112
sequence annotation: BBa_K777121
sequence: https://synbiohub.org/public/igem/BBa_K777123_sequence/1

BBa_J23112
type: Component
persistent identity: component2192772
display id: component2192772
version: 1
top level: BBa_K777123
access: public
definition: BBa_J23112

BBa_K777121
type: Component
persistent identity: component2192774
display id: component2192774
version: 1
top level: BBa_K777123
access: public
definition: https://synbiohub.org/public/igem/BBa_K777121/1

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2192772
display id: annotation2192772
version: 1
top level: BBa_K777123
location: https://synbiohub.org/public/igem/BBa_K777123/annotation2192772/range2192772/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777123/annotation2192772/range2192772/1
type: Range
persistent identity: range2192772
display id: range2192772
version: 1
direction: direction/reverse
top level: BBa_K777123
start: 1
end: 35
orientation: inline

BBa_K777121
type: SequenceAnnotation
persistent identity: annotation2192774
display id: annotation2192774
version: 1
top level: BBa_K777123
location: https://synbiohub.org/public/igem/BBa_K777123/annotation2192774/range2192774/1
component: BBa_K777121

https://synbiohub.org/public/igem/BBa_K777123/annotation2192774/range2192774/1
type: Range
persistent identity: range2192774
display id: range2192774
version: 1
direction: direction/reverse
top level: BBa_K777123
start: 42
end: 809
orientation: inline

BBa_K777125
type: ComponentDefinition
persistent identity: BBa K777125
display id: BBa_K777125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive J23100 promoter
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out J61002 and J23100) and the plasmid backbone pSB1C3.
mutable notes: * No specific design considerations
mutable provenance: * pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: J23100
sequence annotation: constitutive promoter J23100
sequence: https://synbiohub.org/public/igem/BBa_K777125_sequence/1

J23100
type: SequenceAnnotation
persistent identity: annotation2203710
display id: annotation2203710
version: 1
top level: BBa_K777125
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777125/annotation2203710/range2203710/1

https://synbiohub.org/public/igem/BBa_K777125/annotation2203710/range2203710/1
type: Range
persistent identity: range2203710
display id: range2203710
version: 1
direction: direction/forward
top level: BBa_K777125
start: 2
end: 36
orientation: inline

constitutive promoter J23100
type: SequenceAnnotation
persistent identity: annotation2203711
display id: annotation2203711
version: 1
top level: BBa_K777125
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K777125/annotation2203711/range2203711/1

https://synbiohub.org/public/igem/BBa_K777125/annotation2203711/range2203711/1
type: Range
persistent identity: range2203711
display id: range2203711
version: 1
direction: direction/forward
top level: BBa_K777125
start: 2
end: 36
orientation: inline

BBa_K777124
type: ComponentDefinition
persistent identity: BBa K777124
display id: BBa_K777124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
mutable provenance: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777121
component: BBa_J23114
sequence annotation: BBa_K777121
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K777124_sequence/1

BBa_K777121
type: Component
persistent identity: component2193024
display id: component2193024
version: 1
top level: BBa_K777124
access: public
definition: https://synbiohub.org/public/igem/BBa_K777121/1

BBa_J23114
type: Component
persistent identity: component2193022
display id: component2193022
version: 1
top level: BBa_K777124
access: public
definition: BBa_J23114

BBa_K777121
type: SequenceAnnotation
persistent identity: annotation2193024
display id: annotation2193024
version: 1
top level: BBa_K777124
location: https://synbiohub.org/public/igem/BBa_K777124/annotation2193024/range2193024/1
component: BBa_K777121

https://synbiohub.org/public/igem/BBa_K777124/annotation2193024/range2193024/1
type: Range
persistent identity: range2193024
display id: range2193024
version: 1
direction: direction/reverse
top level: BBa_K777124
start: 42
end: 809
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2193022
display id: annotation2193022
version: 1
top level: BBa_K777124
location: https://synbiohub.org/public/igem/BBa_K777124/annotation2193022/range2193022/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777124/annotation2193022/range2193022/1
type: Range
persistent identity: range2193022
display id: range2193022
version: 1
direction: direction/reverse
top level: BBa_K777124
start: 1
end: 35
orientation: inline

BBa_J23118
type: ComponentDefinition
persistent identity: BBa J23118
display id: BBa_J23118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23118_sequence/1

BBa_K777002
type: ComponentDefinition
persistent identity: BBa K777002
display id: BBa_K777002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23104
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23104
component: BBa_K777000
sequence annotation: BBa_K777000
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K777002_sequence/1

BBa_J23104
type: Component
persistent identity: component2189238
display id: component2189238
version: 1
top level: BBa_K777002
access: public
definition: BBa_J23104

BBa_K777000
type: Component
persistent identity: component2189242
display id: component2189242
version: 1
top level: BBa_K777002
access: public
definition: BBa_K777000

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189242
display id: annotation2189242
version: 1
top level: BBa_K777002
location: https://synbiohub.org/public/igem/BBa_K777002/annotation2189242/range2189242/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777002/annotation2189242/range2189242/1
type: Range
persistent identity: range2189242
display id: range2189242
version: 1
direction: direction/reverse
top level: BBa_K777002
start: 42
end: 1703
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2189238
display id: annotation2189238
version: 1
top level: BBa_K777002
location: https://synbiohub.org/public/igem/BBa_K777002/annotation2189238/range2189238/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K777002/annotation2189238/range2189238/1
type: Range
persistent identity: range2189238
display id: range2189238
version: 1
direction: direction/reverse
top level: BBa_K777002
start: 1
end: 35
orientation: inline

BBa_K777131
type: ComponentDefinition
persistent identity: BBa K777131
display id: BBa_K777131
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive J23113 promoter
creator: Team Goettingen
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23113 J23113]) and the plasmid backbone pSB1C3.
mutable notes: * No specific design considerations
mutable provenance: * pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: constitutive promoter J23113
sequence annotation: J23113
sequence: https://synbiohub.org/public/igem/BBa_K777131_sequence/1

constitutive promoter J23113
type: SequenceAnnotation
persistent identity: annotation2204929
display id: annotation2204929
version: 1
top level: BBa_K777131
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K777131/annotation2204929/range2204929/1

https://synbiohub.org/public/igem/BBa_K777131/annotation2204929/range2204929/1
type: Range
persistent identity: range2204929
display id: range2204929
version: 1
direction: direction/forward
top level: BBa_K777131
start: 2
end: 36
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2204928
display id: annotation2204928
version: 1
top level: BBa_K777131
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777131/annotation2204928/range2204928/1

https://synbiohub.org/public/igem/BBa_K777131/annotation2204928/range2204928/1
type: Range
persistent identity: range2204928
display id: range2204928
version: 1
direction: direction/forward
top level: BBa_K777131
start: 2
end: 36
orientation: inline

BBa_K1585102
type: ComponentDefinition
persistent identity: BBa K1585102
display id: BBa_K1585102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:03:57Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23102 J23102] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23102
component: BBa_K1585999
sequence annotation: BBa_J23102
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585102_sequence/1

BBa_J23102
type: Component
persistent identity: component2441417
display id: component2441417
version: 1
top level: BBa_K1585102
access: public
definition: BBa_J23102

BBa_K1585999
type: Component
persistent identity: component2441419
display id: component2441419
version: 1
top level: BBa_K1585102
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2441417
display id: annotation2441417
version: 1
top level: BBa_K1585102
location: https://synbiohub.org/public/igem/BBa_K1585102/annotation2441417/range2441417/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K1585102/annotation2441417/range2441417/1
type: Range
persistent identity: range2441417
display id: range2441417
version: 1
direction: direction/reverse
top level: BBa_K1585102
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441419
display id: annotation2441419
version: 1
top level: BBa_K1585102
location: https://synbiohub.org/public/igem/BBa_K1585102/annotation2441419/range2441419/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585102/annotation2441419/range2441419/1
type: Range
persistent identity: range2441419
display id: range2441419
version: 1
direction: direction/reverse
top level: BBa_K1585102
start: 44
end: 78
orientation: inline

BBa_K777127
type: ComponentDefinition
persistent identity: BBa K777127
display id: BBa_K777127
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive J23105 promoter
creator: Team Goettingen
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23105 J23105]) and the plasmid backbone pSB1C3.
mutable notes: No specific design considerations
mutable provenance: pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: constitutive promoter J23105
sequence annotation: J23105
sequence: https://synbiohub.org/public/igem/BBa_K777127_sequence/1

constitutive promoter J23105
type: SequenceAnnotation
persistent identity: annotation2204849
display id: annotation2204849
version: 1
top level: BBa_K777127
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K777127/annotation2204849/range2204849/1

https://synbiohub.org/public/igem/BBa_K777127/annotation2204849/range2204849/1
type: Range
persistent identity: range2204849
display id: range2204849
version: 1
direction: direction/forward
top level: BBa_K777127
start: 2
end: 36
orientation: inline

J23105
type: SequenceAnnotation
persistent identity: annotation2204848
display id: annotation2204848
version: 1
top level: BBa_K777127
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777127/annotation2204848/range2204848/1

https://synbiohub.org/public/igem/BBa_K777127/annotation2204848/range2204848/1
type: Range
persistent identity: range2204848
display id: range2204848
version: 1
direction: direction/forward
top level: BBa_K777127
start: 2
end: 36
orientation: inline

BBa_K777115
type: ComponentDefinition
persistent identity: BBa K777115
display id: BBa_K777115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
mutable provenance: * ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777113
component: BBa_J23112
sequence annotation: BBa_K777113
sequence annotation: BBa_J23112
sequence: https://synbiohub.org/public/igem/BBa_K777115_sequence/1

BBa_K777113
type: Component
persistent identity: component2189728
display id: component2189728
version: 1
top level: BBa_K777115
access: public
definition: https://synbiohub.org/public/igem/BBa_K777113/1

BBa_J23112
type: Component
persistent identity: component2189726
display id: component2189726
version: 1
top level: BBa_K777115
access: public
definition: BBa_J23112

BBa_K777113
type: SequenceAnnotation
persistent identity: annotation2189728
display id: annotation2189728
version: 1
top level: BBa_K777115
location: https://synbiohub.org/public/igem/BBa_K777115/annotation2189728/range2189728/1
component: BBa_K777113

https://synbiohub.org/public/igem/BBa_K777115/annotation2189728/range2189728/1
type: Range
persistent identity: range2189728
display id: range2189728
version: 1
direction: direction/reverse
top level: BBa_K777115
start: 42
end: 872
orientation: inline

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2189726
display id: annotation2189726
version: 1
top level: BBa_K777115
location: https://synbiohub.org/public/igem/BBa_K777115/annotation2189726/range2189726/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777115/annotation2189726/range2189726/1
type: Range
persistent identity: range2189726
display id: range2189726
version: 1
direction: direction/reverse
top level: BBa_K777115
start: 1
end: 35
orientation: inline

BBa_K1585116
type: ComponentDefinition
persistent identity: BBa K1585116
display id: BBa_K1585116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:19:47Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23116
component: BBa_K1585999
sequence annotation: BBa_J23116
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585116_sequence/1

BBa_J23116
type: Component
persistent identity: component2441708
display id: component2441708
version: 1
top level: BBa_K1585116
access: public
definition: BBa_J23116

BBa_K1585999
type: Component
persistent identity: component2441710
display id: component2441710
version: 1
top level: BBa_K1585116
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2441708
display id: annotation2441708
version: 1
top level: BBa_K1585116
location: https://synbiohub.org/public/igem/BBa_K1585116/annotation2441708/range2441708/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K1585116/annotation2441708/range2441708/1
type: Range
persistent identity: range2441708
display id: range2441708
version: 1
direction: direction/reverse
top level: BBa_K1585116
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441710
display id: annotation2441710
version: 1
top level: BBa_K1585116
location: https://synbiohub.org/public/igem/BBa_K1585116/annotation2441710/range2441710/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585116/annotation2441710/range2441710/1
type: Range
persistent identity: range2441710
display id: range2441710
version: 1
direction: direction/reverse
top level: BBa_K1585116
start: 44
end: 78
orientation: inline

BBa_K777120
type: ComponentDefinition
persistent identity: BBa K777120
display id: BBa_K777120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motB'' expression affect the motility of ''E. coli''.
mutable notes: * ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23114
component: BBa_K777117
sequence annotation: BBa_K777117
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K777120_sequence/1

BBa_J23114
type: Component
persistent identity: component2190799
display id: component2190799
version: 1
top level: BBa_K777120
access: public
definition: BBa_J23114

BBa_K777117
type: Component
persistent identity: component2190801
display id: component2190801
version: 1
top level: BBa_K777120
access: public
definition: https://synbiohub.org/public/igem/BBa_K777117/1

BBa_K777117
type: SequenceAnnotation
persistent identity: annotation2190801
display id: annotation2190801
version: 1
top level: BBa_K777120
location: https://synbiohub.org/public/igem/BBa_K777120/annotation2190801/range2190801/1
component: BBa_K777117

https://synbiohub.org/public/igem/BBa_K777120/annotation2190801/range2190801/1
type: Range
persistent identity: range2190801
display id: range2190801
version: 1
direction: direction/reverse
top level: BBa_K777120
start: 42
end: 968
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2190799
display id: annotation2190799
version: 1
top level: BBa_K777120
location: https://synbiohub.org/public/igem/BBa_K777120/annotation2190799/range2190799/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777120/annotation2190799/range2190799/1
type: Range
persistent identity: range2190799
display id: range2190799
version: 1
direction: direction/reverse
top level: BBa_K777120
start: 1
end: 35
orientation: inline

BBa_K2122000
type: ComponentDefinition
persistent identity: BBa K2122000
display id: BBa_K2122000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter
creator: Sannia Farrukh
created: 2016-09-28T11:00:00Z
modified: 2016-10-18T01:26:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2591_
m user id: 32074
owner id: 32074
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
mutable notes: An inducible promoter (BBa_10500), RBS (BBa_B0034) Scar was added to the sequence. Along with a terminator.
mutable provenance: Coding Sequence from GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K2122150
component: BBa_B0014
component: BBa_I0500
component: BBa_B0034
sequence annotation: BBa_B0014
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K2122150
sequence: https://synbiohub.org/public/igem/BBa_K2122000_sequence/1

BBa_K2122150
type: Component
persistent identity: component2517225
display id: component2517225
version: 1
top level: BBa_K2122000
access: public
definition: https://synbiohub.org/public/igem/BBa_K2122150/1

BBa_B0014
type: Component
persistent identity: component2517232
display id: component2517232
version: 1
top level: BBa_K2122000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_I0500
type: Component
persistent identity: component2517222
display id: component2517222
version: 1
top level: BBa_K2122000
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2517224
display id: component2517224
version: 1
top level: BBa_K2122000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2517232
display id: annotation2517232
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517232/range2517232/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517232/range2517232/1
type: Range
persistent identity: range2517232
display id: range2517232
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 2307
end: 2401
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2517222
display id: annotation2517222
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517222/range2517222/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517222/range2517222/1
type: Range
persistent identity: range2517222
display id: range2517222
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2517224
display id: annotation2517224
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517224/range2517224/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517224/range2517224/1
type: Range
persistent identity: range2517224
display id: range2517224
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 1219
end: 1230
orientation: inline

BBa_K2122150
type: SequenceAnnotation
persistent identity: annotation2517225
display id: annotation2517225
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517225/range2517225/1
component: BBa_K2122150

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517225/range2517225/1
type: Range
persistent identity: range2517225
display id: range2517225
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 1237
end: 2298
orientation: inline

BBa_K777005
type: ComponentDefinition
persistent identity: BBa K777005
display id: BBa_K777005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23109
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23109 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23109 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777000
component: BBa_J23109
sequence annotation: BBa_K777000
sequence annotation: BBa_J23109
sequence: https://synbiohub.org/public/igem/BBa_K777005_sequence/1

BBa_K777000
type: Component
persistent identity: component2189372
display id: component2189372
version: 1
top level: BBa_K777005
access: public
definition: BBa_K777000

BBa_J23109
type: Component
persistent identity: component2189368
display id: component2189368
version: 1
top level: BBa_K777005
access: public
definition: BBa_J23109

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189372
display id: annotation2189372
version: 1
top level: BBa_K777005
location: https://synbiohub.org/public/igem/BBa_K777005/annotation2189372/range2189372/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777005/annotation2189372/range2189372/1
type: Range
persistent identity: range2189372
display id: range2189372
version: 1
direction: direction/reverse
top level: BBa_K777005
start: 42
end: 1703
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2189368
display id: annotation2189368
version: 1
top level: BBa_K777005
location: https://synbiohub.org/public/igem/BBa_K777005/annotation2189368/range2189368/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K777005/annotation2189368/range2189368/1
type: Range
persistent identity: range2189368
display id: range2189368
version: 1
direction: direction/reverse
top level: BBa_K777005
start: 1
end: 35
orientation: inline

BBa_K823007
type: ComponentDefinition
persistent identity: BBa K823007
display id: BBa_K823007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23103
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:46:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23103 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: J23103
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823007_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182517
display id: annotation2182517
version: 1
top level: BBa_K823007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182517/range2182517/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182517/range2182517/1
type: Range
persistent identity: range2182517
display id: range2182517
version: 1
direction: direction/forward
top level: BBa_K823007
start: 24
end: 29
orientation: inline

J23103
type: SequenceAnnotation
persistent identity: annotation2182515
display id: annotation2182515
version: 1
top level: BBa_K823007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182515/range2182515/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182515/range2182515/1
type: Range
persistent identity: range2182515
display id: range2182515
version: 1
direction: direction/forward
top level: BBa_K823007
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182516
display id: annotation2182516
version: 1
top level: BBa_K823007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182516/range2182516/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182516/range2182516/1
type: Range
persistent identity: range2182516
display id: range2182516
version: 1
direction: direction/forward
top level: BBa_K823007
start: 1
end: 6
orientation: inline

BBa_K777122
type: ComponentDefinition
persistent identity: BBa K777122
display id: BBa_K777122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
mutable provenance: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K777121
component: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_K777121
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K777122_sequence/1

BBa_J23100
type: Component
persistent identity: component2191153
display id: component2191153
version: 1
top level: BBa_K777122
access: public
definition: BBa_J23100

BBa_K777121
type: Component
persistent identity: component2191157
display id: component2191157
version: 1
top level: BBa_K777122
access: public
definition: https://synbiohub.org/public/igem/BBa_K777121/1

BBa_B0034
type: Component
persistent identity: component2191155
display id: component2191155
version: 1
top level: BBa_K777122
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2191153
display id: annotation2191153
version: 1
top level: BBa_K777122
location: https://synbiohub.org/public/igem/BBa_K777122/annotation2191153/range2191153/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777122/annotation2191153/range2191153/1
type: Range
persistent identity: range2191153
display id: range2191153
version: 1
direction: direction/reverse
top level: BBa_K777122
start: 1
end: 35
orientation: inline

BBa_K777121
type: SequenceAnnotation
persistent identity: annotation2191157
display id: annotation2191157
version: 1
top level: BBa_K777122
location: https://synbiohub.org/public/igem/BBa_K777122/annotation2191157/range2191157/1
component: BBa_K777121

https://synbiohub.org/public/igem/BBa_K777122/annotation2191157/range2191157/1
type: Range
persistent identity: range2191157
display id: range2191157
version: 1
direction: direction/reverse
top level: BBa_K777122
start: 62
end: 829
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2191155
display id: annotation2191155
version: 1
top level: BBa_K777122
location: https://synbiohub.org/public/igem/BBa_K777122/annotation2191155/range2191155/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K777122/annotation2191155/range2191155/1
type: Range
persistent identity: range2191155
display id: range2191155
version: 1
direction: direction/reverse
top level: BBa_K777122
start: 44
end: 55
orientation: inline

BBa_J23112
type: ComponentDefinition
persistent identity: BBa J23112
display id: BBa_J23112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23112_sequence/1

BBa_K777106
type: ComponentDefinition
persistent identity: BBa K777106
display id: BBa_K777106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23112
sequence annotation: BBa_K777100
sequence annotation: BBa_J23112
sequence: https://synbiohub.org/public/igem/BBa_K777106_sequence/1

BBa_K777100
type: Component
persistent identity: component2187094
display id: component2187094
version: 1
top level: BBa_K777106
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23112
type: Component
persistent identity: component2187086
display id: component2187086
version: 1
top level: BBa_K777106
access: public
definition: BBa_J23112

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187094
display id: annotation2187094
version: 1
top level: BBa_K777106
location: https://synbiohub.org/public/igem/BBa_K777106/annotation2187094/range2187094/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777106/annotation2187094/range2187094/1
type: Range
persistent identity: range2187094
display id: range2187094
version: 1
direction: direction/reverse
top level: BBa_K777106
start: 42
end: 910
orientation: inline

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2187086
display id: annotation2187086
version: 1
top level: BBa_K777106
location: https://synbiohub.org/public/igem/BBa_K777106/annotation2187086/range2187086/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777106/annotation2187086/range2187086/1
type: Range
persistent identity: range2187086
display id: range2187086
version: 1
direction: direction/reverse
top level: BBa_K777106
start: 1
end: 35
orientation: inline

BBa_K1585104
type: ComponentDefinition
persistent identity: BBa K1585104
display id: BBa_K1585104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T03:26:03Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23104 J23104] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23104
component: BBa_K1585999
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K1585104_sequence/1

BBa_J23104
type: Component
persistent identity: component2441420
display id: component2441420
version: 1
top level: BBa_K1585104
access: public
definition: BBa_J23104

BBa_K1585999
type: Component
persistent identity: component2441422
display id: component2441422
version: 1
top level: BBa_K1585104
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441422
display id: annotation2441422
version: 1
top level: BBa_K1585104
location: https://synbiohub.org/public/igem/BBa_K1585104/annotation2441422/range2441422/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585104/annotation2441422/range2441422/1
type: Range
persistent identity: range2441422
display id: range2441422
version: 1
direction: direction/reverse
top level: BBa_K1585104
start: 44
end: 78
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2441420
display id: annotation2441420
version: 1
top level: BBa_K1585104
location: https://synbiohub.org/public/igem/BBa_K1585104/annotation2441420/range2441420/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K1585104/annotation2441420/range2441420/1
type: Range
persistent identity: range2441420
display id: range2441420
version: 1
direction: direction/reverse
top level: BBa_K1585104
start: 1
end: 35
orientation: inline

BBa_K1585103
type: ComponentDefinition
persistent identity: BBa K1585103
display id: BBa_K1585103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T03:08:18Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23103
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23103
sequence: https://synbiohub.org/public/igem/BBa_K1585103_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441413
display id: component2441413
version: 1
top level: BBa_K1585103
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23103
type: Component
persistent identity: component2441411
display id: component2441411
version: 1
top level: BBa_K1585103
access: public
definition: BBa_J23103

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441413
display id: annotation2441413
version: 1
top level: BBa_K1585103
location: https://synbiohub.org/public/igem/BBa_K1585103/annotation2441413/range2441413/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585103/annotation2441413/range2441413/1
type: Range
persistent identity: range2441413
display id: range2441413
version: 1
direction: direction/reverse
top level: BBa_K1585103
start: 44
end: 78
orientation: inline

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2441411
display id: annotation2441411
version: 1
top level: BBa_K1585103
location: https://synbiohub.org/public/igem/BBa_K1585103/annotation2441411/range2441411/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K1585103/annotation2441411/range2441411/1
type: Range
persistent identity: range2441411
display id: range2441411
version: 1
direction: direction/reverse
top level: BBa_K1585103
start: 1
end: 35
orientation: inline

BBa_K1679004
type: ComponentDefinition
persistent identity: BBa K1679004
display id: BBa_K1679004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101 and RiboJ
creator: Qikai Qin
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T06:26:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2097_
m user id: 25072
owner id: 21726
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BBa_J23101 is a strong promoter which is well characterized by many teams who take part in 2015 InterLab Study. RiboJ is a Ribozyme-based insulator part that can buffer synthetic circuits from genetic context. This part can be used as a promoter-like parts.
mutable notes: We want to make a promoter part containing RiboJ to buffer synthetic circuits from genetic context. We synthesized RiboJ with EXSP site and use 3A Assembly to construct this part.
mutable provenance: BBa_J23101 We synthesized RiboJ. Our reference is Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1679038
component: BBa_J23101
sequence annotation: BBa_J23101
sequence annotation: BBa_K1679038
sequence: https://synbiohub.org/public/igem/BBa_K1679004_sequence/1

BBa_K1679038
type: Component
persistent identity: component2474697
display id: component2474697
version: 1
top level: BBa_K1679004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1679038/1

BBa_J23101
type: Component
persistent identity: component2474696
display id: component2474696
version: 1
top level: BBa_K1679004
access: public
definition: BBa_J23101

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2474696
display id: annotation2474696
version: 1
top level: BBa_K1679004
location: https://synbiohub.org/public/igem/BBa_K1679004/annotation2474696/range2474696/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1679004/annotation2474696/range2474696/1
type: Range
persistent identity: range2474696
display id: range2474696
version: 1
direction: direction/reverse
top level: BBa_K1679004
start: 1
end: 35
orientation: inline

BBa_K1679038
type: SequenceAnnotation
persistent identity: annotation2474697
display id: annotation2474697
version: 1
top level: BBa_K1679004
location: https://synbiohub.org/public/igem/BBa_K1679004/annotation2474697/range2474697/1
component: BBa_K1679038

https://synbiohub.org/public/igem/BBa_K1679004/annotation2474697/range2474697/1
type: Range
persistent identity: range2474697
display id: range2474697
version: 1
direction: direction/reverse
top level: BBa_K1679004
start: 44
end: 118
orientation: inline

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

BBa_J23117
type: ComponentDefinition
persistent identity: BBa J23117
display id: BBa_J23117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23117_sequence/1

BBa_K823014
type: ComponentDefinition
persistent identity: BBa K823014
display id: BBa_K823014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23118
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:51:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23118 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23118
sequence: https://synbiohub.org/public/igem/BBa_K823014_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182547
display id: annotation2182547
version: 1
top level: BBa_K823014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182547/range2182547/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182547/range2182547/1
type: Range
persistent identity: range2182547
display id: range2182547
version: 1
direction: direction/forward
top level: BBa_K823014
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182548
display id: annotation2182548
version: 1
top level: BBa_K823014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182548/range2182548/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182548/range2182548/1
type: Range
persistent identity: range2182548
display id: range2182548
version: 1
direction: direction/forward
top level: BBa_K823014
start: 24
end: 29
orientation: inline

J23118
type: SequenceAnnotation
persistent identity: annotation2182546
display id: annotation2182546
version: 1
top level: BBa_K823014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182546/range2182546/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182546/range2182546/1
type: Range
persistent identity: range2182546
display id: range2182546
version: 1
direction: direction/forward
top level: BBa_K823014
start: 1
end: 35
orientation: inline

BBa_K777119
type: ComponentDefinition
persistent identity: BBa K777119
display id: BBa_K777119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motB'' expression affect the motility of ''E. coli''.
mutable notes: * ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777117
component: BBa_J23112
sequence annotation: BBa_K777117
sequence annotation: BBa_J23112
sequence: https://synbiohub.org/public/igem/BBa_K777119_sequence/1

BBa_K777117
type: Component
persistent identity: component2190798
display id: component2190798
version: 1
top level: BBa_K777119
access: public
definition: https://synbiohub.org/public/igem/BBa_K777117/1

BBa_J23112
type: Component
persistent identity: component2190796
display id: component2190796
version: 1
top level: BBa_K777119
access: public
definition: BBa_J23112

BBa_K777117
type: SequenceAnnotation
persistent identity: annotation2190798
display id: annotation2190798
version: 1
top level: BBa_K777119
location: https://synbiohub.org/public/igem/BBa_K777119/annotation2190798/range2190798/1
component: BBa_K777117

https://synbiohub.org/public/igem/BBa_K777119/annotation2190798/range2190798/1
type: Range
persistent identity: range2190798
display id: range2190798
version: 1
direction: direction/reverse
top level: BBa_K777119
start: 42
end: 968
orientation: inline

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2190796
display id: annotation2190796
version: 1
top level: BBa_K777119
location: https://synbiohub.org/public/igem/BBa_K777119/annotation2190796/range2190796/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777119/annotation2190796/range2190796/1
type: Range
persistent identity: range2190796
display id: range2190796
version: 1
direction: direction/reverse
top level: BBa_K777119
start: 1
end: 35
orientation: inline

BBa_K1468002
type: ComponentDefinition
persistent identity: BBa K1468002
display id: BBa_K1468002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23110 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468002_sequence/1

BBa_J23114
type: ComponentDefinition
persistent identity: BBa J23114
display id: BBa_J23114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23114_sequence/1

BBa_K777105
type: ComponentDefinition
persistent identity: BBa K777105
display id: BBa_K777105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23109
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23109 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23109
component: BBa_K777100
sequence annotation: BBa_K777100
sequence annotation: BBa_J23109
sequence: https://synbiohub.org/public/igem/BBa_K777105_sequence/1

BBa_J23109
type: Component
persistent identity: component2187048
display id: component2187048
version: 1
top level: BBa_K777105
access: public
definition: BBa_J23109

BBa_K777100
type: Component
persistent identity: component2187056
display id: component2187056
version: 1
top level: BBa_K777105
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187056
display id: annotation2187056
version: 1
top level: BBa_K777105
location: https://synbiohub.org/public/igem/BBa_K777105/annotation2187056/range2187056/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777105/annotation2187056/range2187056/1
type: Range
persistent identity: range2187056
display id: range2187056
version: 1
direction: direction/reverse
top level: BBa_K777105
start: 42
end: 910
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2187048
display id: annotation2187048
version: 1
top level: BBa_K777105
location: https://synbiohub.org/public/igem/BBa_K777105/annotation2187048/range2187048/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K777105/annotation2187048/range2187048/1
type: Range
persistent identity: range2187048
display id: range2187048
version: 1
direction: direction/reverse
top level: BBa_K777105
start: 1
end: 35
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_J23109
type: ComponentDefinition
persistent identity: BBa J23109
display id: BBa_J23109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23109_sequence/1

BBa_K1585118
type: ComponentDefinition
persistent identity: BBa K1585118
display id: BBa_K1585118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:31:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23118 J23118] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23118
sequence annotation: BBa_J23118
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585118_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441717
display id: component2441717
version: 1
top level: BBa_K1585118
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23118
type: Component
persistent identity: component2441715
display id: component2441715
version: 1
top level: BBa_K1585118
access: public
definition: BBa_J23118

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation2441715
display id: annotation2441715
version: 1
top level: BBa_K1585118
location: https://synbiohub.org/public/igem/BBa_K1585118/annotation2441715/range2441715/1
component: BBa_J23118

https://synbiohub.org/public/igem/BBa_K1585118/annotation2441715/range2441715/1
type: Range
persistent identity: range2441715
display id: range2441715
version: 1
direction: direction/reverse
top level: BBa_K1585118
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441717
display id: annotation2441717
version: 1
top level: BBa_K1585118
location: https://synbiohub.org/public/igem/BBa_K1585118/annotation2441717/range2441717/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585118/annotation2441717/range2441717/1
type: Range
persistent identity: range2441717
display id: range2441717
version: 1
direction: direction/reverse
top level: BBa_K1585118
start: 44
end: 78
orientation: inline

BBa_K1468001
type: ComponentDefinition
persistent identity: BBa K1468001
display id: BBa_K1468001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding AsRed2
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468001_sequence/1

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

BBa_K1585119
type: ComponentDefinition
persistent identity: BBa K1585119
display id: BBa_K1585119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:33:36Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23119 J23119] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23119
sequence annotation: BBa_J23119
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585119_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441721
display id: component2441721
version: 1
top level: BBa_K1585119
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23119
type: Component
persistent identity: component2441719
display id: component2441719
version: 1
top level: BBa_K1585119
access: public
definition: BBa_J23119

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2441719
display id: annotation2441719
version: 1
top level: BBa_K1585119
location: https://synbiohub.org/public/igem/BBa_K1585119/annotation2441719/range2441719/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1585119/annotation2441719/range2441719/1
type: Range
persistent identity: range2441719
display id: range2441719
version: 1
direction: direction/reverse
top level: BBa_K1585119
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441721
display id: annotation2441721
version: 1
top level: BBa_K1585119
location: https://synbiohub.org/public/igem/BBa_K1585119/annotation2441721/range2441721/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585119/annotation2441721/range2441721/1
type: Range
persistent identity: range2441721
display id: range2441721
version: 1
direction: direction/reverse
top level: BBa_K1585119
start: 44
end: 78
orientation: inline

BBa_K1585100
type: ComponentDefinition
persistent identity: BBa K1585100
display id: BBa_K1585100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-23T11:00:00Z
modified: 2015-08-26T11:28:17Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same. We choosed part BBa_R0010 because we assumed older parts would be reliable.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23100
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1585100_sequence/1

BBa_K1585999
type: Component
persistent identity: component2436913
display id: component2436913
version: 1
top level: BBa_K1585100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23100
type: Component
persistent identity: component2436912
display id: component2436912
version: 1
top level: BBa_K1585100
access: public
definition: BBa_J23100

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2436913
display id: annotation2436913
version: 1
top level: BBa_K1585100
location: https://synbiohub.org/public/igem/BBa_K1585100/annotation2436913/range2436913/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585100/annotation2436913/range2436913/1
type: Range
persistent identity: range2436913
display id: range2436913
version: 1
direction: direction/reverse
top level: BBa_K1585100
start: 44
end: 78
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2436912
display id: annotation2436912
version: 1
top level: BBa_K1585100
location: https://synbiohub.org/public/igem/BBa_K1585100/annotation2436912/range2436912/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1585100/annotation2436912/range2436912/1
type: Range
persistent identity: range2436912
display id: range2436912
version: 1
direction: direction/reverse
top level: BBa_K1585100
start: 1
end: 35
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Replace later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K823010
type: ComponentDefinition
persistent identity: BBa K823010
display id: BBa_K823010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23113
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23113 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: -35 box
sequence annotation: J23113
sequence: https://synbiohub.org/public/igem/BBa_K823010_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182536
display id: annotation2182536
version: 1
top level: BBa_K823010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182536/range2182536/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182536/range2182536/1
type: Range
persistent identity: range2182536
display id: range2182536
version: 1
direction: direction/forward
top level: BBa_K823010
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182535
display id: annotation2182535
version: 1
top level: BBa_K823010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182535/range2182535/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182535/range2182535/1
type: Range
persistent identity: range2182535
display id: range2182535
version: 1
direction: direction/forward
top level: BBa_K823010
start: 1
end: 6
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2182534
display id: annotation2182534
version: 1
top level: BBa_K823010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182534/range2182534/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182534/range2182534/1
type: Range
persistent identity: range2182534
display id: range2182534
version: 1
direction: direction/forward
top level: BBa_K823010
start: 1
end: 35
orientation: inline

BBa_J23113
type: ComponentDefinition
persistent identity: BBa J23113
display id: BBa_J23113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23113_sequence/1

BBa_K1585113
type: ComponentDefinition
persistent identity: BBa K1585113
display id: BBa_K1585113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:09:36Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23110 J23110] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23113
sequence annotation: BBa_J23113
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585113_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441704
display id: component2441704
version: 1
top level: BBa_K1585113
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23113
type: Component
persistent identity: component2441702
display id: component2441702
version: 1
top level: BBa_K1585113
access: public
definition: BBa_J23113

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2441702
display id: annotation2441702
version: 1
top level: BBa_K1585113
location: https://synbiohub.org/public/igem/BBa_K1585113/annotation2441702/range2441702/1
component: BBa_J23113

https://synbiohub.org/public/igem/BBa_K1585113/annotation2441702/range2441702/1
type: Range
persistent identity: range2441702
display id: range2441702
version: 1
direction: direction/reverse
top level: BBa_K1585113
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441704
display id: annotation2441704
version: 1
top level: BBa_K1585113
location: https://synbiohub.org/public/igem/BBa_K1585113/annotation2441704/range2441704/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585113/annotation2441704/range2441704/1
type: Range
persistent identity: range2441704
display id: range2441704
version: 1
direction: direction/reverse
top level: BBa_K1585113
start: 44
end: 78
orientation: inline

BBa_K777107
type: ComponentDefinition
persistent identity: BBa K777107
display id: BBa_K777107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23113
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23113 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23113
sequence annotation: BBa_J23113
sequence annotation: BBa_K777100
sequence: https://synbiohub.org/public/igem/BBa_K777107_sequence/1

BBa_K777100
type: Component
persistent identity: component2187103
display id: component2187103
version: 1
top level: BBa_K777107
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23113
type: Component
persistent identity: component2187095
display id: component2187095
version: 1
top level: BBa_K777107
access: public
definition: BBa_J23113

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2187095
display id: annotation2187095
version: 1
top level: BBa_K777107
location: https://synbiohub.org/public/igem/BBa_K777107/annotation2187095/range2187095/1
component: BBa_J23113

https://synbiohub.org/public/igem/BBa_K777107/annotation2187095/range2187095/1
type: Range
persistent identity: range2187095
display id: range2187095
version: 1
direction: direction/reverse
top level: BBa_K777107
start: 1
end: 35
orientation: inline

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187103
display id: annotation2187103
version: 1
top level: BBa_K777107
location: https://synbiohub.org/public/igem/BBa_K777107/annotation2187103/range2187103/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777107/annotation2187103/range2187103/1
type: Range
persistent identity: range2187103
display id: range2187103
version: 1
direction: direction/reverse
top level: BBa_K777107
start: 42
end: 910
orientation: inline

BBa_J23102
type: ComponentDefinition
persistent identity: BBa J23102
display id: BBa_J23102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23102_sequence/1

BBa_K1824896
type: ComponentDefinition
persistent identity: BBa K1824896
display id: BBa_K1824896
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + RBS
creator: Xinhao Wang
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T09:57:14Z
discontinued: false
dominant: false
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Constitutive promoter J23100 plus RBS.
mutable notes: no special concern
mutable provenance: Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. B0030 is a strong RBS based on Ron Weiss thesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1824890
component: BBa_K1824889
component: BBa_B0030
component: BBa_J23100
sequence annotation: BBa_K1824890
sequence annotation: BBa_B0030
sequence annotation: BBa_K1824889
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1824896_sequence/1

BBa_K1824890
type: Component
persistent identity: component2452345
display id: component2452345
version: 1
top level: BBa_K1824896
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_K1824889
type: Component
persistent identity: component2452341
display id: component2452341
version: 1
top level: BBa_K1824896
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_B0030
type: Component
persistent identity: component2452343
display id: component2452343
version: 1
top level: BBa_K1824896
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J23100
type: Component
persistent identity: component2452340
display id: component2452340
version: 1
top level: BBa_K1824896
access: public
definition: BBa_J23100

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2452345
display id: annotation2452345
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452345/range2452345/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452345/range2452345/1
type: Range
persistent identity: range2452345
display id: range2452345
version: 1
direction: direction/reverse
top level: BBa_K1824896
start: 83
end: 88
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2452343
display id: annotation2452343
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452343/range2452343/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452343/range2452343/1
type: Range
persistent identity: range2452343
display id: range2452343
version: 1
direction: direction/forward
top level: BBa_K1824896
start: 60
end: 74
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2452341
display id: annotation2452341
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452341/range2452341/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452341/range2452341/1
type: Range
persistent identity: range2452341
display id: range2452341
version: 1
direction: direction/reverse
top level: BBa_K1824896
start: 44
end: 51
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2452340
display id: annotation2452340
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452340/range2452340/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452340/range2452340/1
type: Range
persistent identity: range2452340
display id: range2452340
version: 1
direction: direction/reverse
top level: BBa_K1824896
start: 1
end: 35
orientation: inline

BBa_K777007
type: ComponentDefinition
persistent identity: BBa K777007
display id: BBa_K777007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23113
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23113 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23113 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23113
component: BBa_K777000
sequence annotation: BBa_J23113
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K777007_sequence/1

BBa_J23113
type: Component
persistent identity: component2189399
display id: component2189399
version: 1
top level: BBa_K777007
access: public
definition: BBa_J23113

BBa_K777000
type: Component
persistent identity: component2189403
display id: component2189403
version: 1
top level: BBa_K777007
access: public
definition: BBa_K777000

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2189399
display id: annotation2189399
version: 1
top level: BBa_K777007
location: https://synbiohub.org/public/igem/BBa_K777007/annotation2189399/range2189399/1
component: BBa_J23113

https://synbiohub.org/public/igem/BBa_K777007/annotation2189399/range2189399/1
type: Range
persistent identity: range2189399
display id: range2189399
version: 1
direction: direction/reverse
top level: BBa_K777007
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189403
display id: annotation2189403
version: 1
top level: BBa_K777007
location: https://synbiohub.org/public/igem/BBa_K777007/annotation2189403/range2189403/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777007/annotation2189403/range2189403/1
type: Range
persistent identity: range2189403
display id: range2189403
version: 1
direction: direction/reverse
top level: BBa_K777007
start: 42
end: 1703
orientation: inline

BBa_K292001
type: ComponentDefinition
persistent identity: BBa K292001
display id: BBa_K292001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double terminator + Constitutive promoter + Strong RBS
creator: David Charoy
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a double terminator and a constitutive prokaryotic promoter and a strong RBS (BBa_B0014 + BBa_J23100 + BBa_B0030). It is a simple promoter in the goals to begin gene transcription. For Sup???biotech-Paris (2009) team this part is used when adding to a recombinant lambda phage and to begin the transcription of synthetic genes inside the Lambda phage genome.
mutable notes: N/A
mutable provenance: BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16). BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04.) BBa_B0030 : Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0011
component: BBa_B0030
component: BBa_J23100
component: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_J23100
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K292001_sequence/1

BBa_B0011
type: Component
persistent identity: component2052037
display id: component2052037
version: 1
top level: BBa_K292001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0030
type: Component
persistent identity: component2052041
display id: component2052041
version: 1
top level: BBa_K292001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J23100
type: Component
persistent identity: component2052039
display id: component2052039
version: 1
top level: BBa_K292001
access: public
definition: BBa_J23100

BBa_B0012
type: Component
persistent identity: component2052033
display id: component2052033
version: 1
top level: BBa_K292001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2052037
display id: annotation2052037
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052037/range2052037/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292001/annotation2052037/range2052037/1
type: Range
persistent identity: range2052037
display id: range2052037
version: 1
direction: direction/forward
top level: BBa_K292001
start: 50
end: 95
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2052039
display id: annotation2052039
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052039/range2052039/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K292001/annotation2052039/range2052039/1
type: Range
persistent identity: range2052039
display id: range2052039
version: 1
direction: direction/reverse
top level: BBa_K292001
start: 104
end: 138
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2052041
display id: annotation2052041
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052041/range2052041/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K292001/annotation2052041/range2052041/1
type: Range
persistent identity: range2052041
display id: range2052041
version: 1
direction: direction/forward
top level: BBa_K292001
start: 147
end: 161
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2052033
display id: annotation2052033
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052033/range2052033/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292001/annotation2052033/range2052033/1
type: Range
persistent identity: range2052033
display id: range2052033
version: 1
direction: direction/forward
top level: BBa_K292001
start: 1
end: 41
orientation: inline

BBa_K823008
type: ComponentDefinition
persistent identity: BBa K823008
display id: BBa_K823008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23106
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23106
sequence: https://synbiohub.org/public/igem/BBa_K823008_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182528
display id: annotation2182528
version: 1
top level: BBa_K823008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1
type: Range
persistent identity: range2182528
display id: range2182528
version: 1
direction: direction/forward
top level: BBa_K823008
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182529
display id: annotation2182529
version: 1
top level: BBa_K823008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1
type: Range
persistent identity: range2182529
display id: range2182529
version: 1
direction: direction/forward
top level: BBa_K823008
start: 24
end: 29
orientation: inline

J23106
type: SequenceAnnotation
persistent identity: annotation2182530
display id: annotation2182530
version: 1
top level: BBa_K823008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1
type: Range
persistent identity: range2182530
display id: range2182530
version: 1
direction: direction/forward
top level: BBa_K823008
start: 1
end: 35
orientation: inline

BBa_K777130
type: ComponentDefinition
persistent identity: BBa K777130
display id: BBa_K777130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive J23112 promoter
creator: Team Goettingen
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23112 J23112]) and the plasmid backbone pSB1C3.
mutable notes: * No specific design considerations
mutable provenance: * pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: J23112
sequence annotation: constitutive promoter J23112
sequence: https://synbiohub.org/public/igem/BBa_K777130_sequence/1

J23112
type: SequenceAnnotation
persistent identity: annotation2204908
display id: annotation2204908
version: 1
top level: BBa_K777130
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777130/annotation2204908/range2204908/1

https://synbiohub.org/public/igem/BBa_K777130/annotation2204908/range2204908/1
type: Range
persistent identity: range2204908
display id: range2204908
version: 1
direction: direction/forward
top level: BBa_K777130
start: 2
end: 36
orientation: inline

constitutive promoter J23112
type: SequenceAnnotation
persistent identity: annotation2204909
display id: annotation2204909
version: 1
top level: BBa_K777130
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K777130/annotation2204909/range2204909/1

https://synbiohub.org/public/igem/BBa_K777130/annotation2204909/range2204909/1
type: Range
persistent identity: range2204909
display id: range2204909
version: 1
direction: direction/forward
top level: BBa_K777130
start: 2
end: 36
orientation: inline

BBa_K777126
type: ComponentDefinition
persistent identity: BBa K777126
display id: BBa_K777126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive J23104 promoter
creator: Team Goettingen
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23100 J23100]) and the plasmid backbone pSB1C3.
mutable notes: No specific design considerations
mutable provenance: pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: constitutive promoter J23104
sequence annotation: J23104
sequence: https://synbiohub.org/public/igem/BBa_K777126_sequence/1

constitutive promoter J23104
type: SequenceAnnotation
persistent identity: annotation2204829
display id: annotation2204829
version: 1
top level: BBa_K777126
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K777126/annotation2204829/range2204829/1

https://synbiohub.org/public/igem/BBa_K777126/annotation2204829/range2204829/1
type: Range
persistent identity: range2204829
display id: range2204829
version: 1
direction: direction/forward
top level: BBa_K777126
start: 2
end: 36
orientation: inline

J23104
type: SequenceAnnotation
persistent identity: annotation2204828
display id: annotation2204828
version: 1
top level: BBa_K777126
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777126/annotation2204828/range2204828/1

https://synbiohub.org/public/igem/BBa_K777126/annotation2204828/range2204828/1
type: Range
persistent identity: range2204828
display id: range2204828
version: 1
direction: direction/forward
top level: BBa_K777126
start: 2
end: 36
orientation: inline

BBa_K1585110
type: ComponentDefinition
persistent identity: BBa K1585110
display id: BBa_K1585110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:09:56Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23106 J23106] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23110
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K1585110_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441701
display id: component2441701
version: 1
top level: BBa_K1585110
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23110
type: Component
persistent identity: component2441699
display id: component2441699
version: 1
top level: BBa_K1585110
access: public
definition: BBa_J23110

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441701
display id: annotation2441701
version: 1
top level: BBa_K1585110
location: https://synbiohub.org/public/igem/BBa_K1585110/annotation2441701/range2441701/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585110/annotation2441701/range2441701/1
type: Range
persistent identity: range2441701
display id: range2441701
version: 1
direction: direction/reverse
top level: BBa_K1585110
start: 44
end: 78
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2441699
display id: annotation2441699
version: 1
top level: BBa_K1585110
location: https://synbiohub.org/public/igem/BBa_K1585110/annotation2441699/range2441699/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1585110/annotation2441699/range2441699/1
type: Range
persistent identity: range2441699
display id: range2441699
version: 1
direction: direction/reverse
top level: BBa_K1585110
start: 1
end: 35
orientation: inline

BBa_K777114
type: ComponentDefinition
persistent identity: BBa K777114
display id: BBa_K777114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
mutable provenance: * ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777113
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K777113
sequence: https://synbiohub.org/public/igem/BBa_K777114_sequence/1

BBa_K777113
type: Component
persistent identity: component2189707
display id: component2189707
version: 1
top level: BBa_K777114
access: public
definition: https://synbiohub.org/public/igem/BBa_K777113/1

BBa_J23100
type: Component
persistent identity: component2189705
display id: component2189705
version: 1
top level: BBa_K777114
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189705
display id: annotation2189705
version: 1
top level: BBa_K777114
location: https://synbiohub.org/public/igem/BBa_K777114/annotation2189705/range2189705/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777114/annotation2189705/range2189705/1
type: Range
persistent identity: range2189705
display id: range2189705
version: 1
direction: direction/reverse
top level: BBa_K777114
start: 1
end: 35
orientation: inline

BBa_K777113
type: SequenceAnnotation
persistent identity: annotation2189707
display id: annotation2189707
version: 1
top level: BBa_K777114
location: https://synbiohub.org/public/igem/BBa_K777114/annotation2189707/range2189707/1
component: BBa_K777113

https://synbiohub.org/public/igem/BBa_K777114/annotation2189707/range2189707/1
type: Range
persistent identity: range2189707
display id: range2189707
version: 1
direction: direction/reverse
top level: BBa_K777114
start: 42
end: 872
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: N/A
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

CP1LacPI2
type: ComponentDefinition
persistent identity: BBa K418002
display id: BBa_K418002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6938
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23100
component: BBa_Q04121
sequence annotation: BBa_J23100
sequence annotation: BBa_Q04121
sequence: https://synbiohub.org/public/igem/BBa_K418002_sequence/1

BBa_J23100
type: Component
persistent identity: component2263743
display id: component2263743
version: 1
top level: CP1LacPI2
access: public
definition: BBa_J23100

BBa_Q04121
type: Component
persistent identity: component2263762
display id: component2263762
version: 1
top level: CP1LacPI2
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2263743
display id: annotation2263743
version: 1
top level: CP1LacPI2
location: https://synbiohub.org/public/igem/BBa_K418002/annotation2263743/range2263743/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K418002/annotation2263743/range2263743/1
type: Range
persistent identity: range2263743
display id: range2263743
version: 1
direction: direction/reverse
top level: CP1LacPI2
start: 1
end: 35
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2263762
display id: annotation2263762
version: 1
top level: CP1LacPI2
location: https://synbiohub.org/public/igem/BBa_K418002/annotation2263762/range2263762/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K418002/annotation2263762/range2263762/1
type: Range
persistent identity: range2263762
display id: range2263762
version: 1
direction: direction/reverse
top level: CP1LacPI2
start: 44
end: 1414
orientation: inline

BBa_K777003
type: ComponentDefinition
persistent identity: BBa K777003
display id: BBa_K777003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23105
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23105 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23105 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23105
component: BBa_K777000
sequence annotation: BBa_J23105
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K777003_sequence/1

BBa_J23105
type: Component
persistent identity: component2189293
display id: component2189293
version: 1
top level: BBa_K777003
access: public
definition: BBa_J23105

BBa_K777000
type: Component
persistent identity: component2189297
display id: component2189297
version: 1
top level: BBa_K777003
access: public
definition: BBa_K777000

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2189293
display id: annotation2189293
version: 1
top level: BBa_K777003
location: https://synbiohub.org/public/igem/BBa_K777003/annotation2189293/range2189293/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K777003/annotation2189293/range2189293/1
type: Range
persistent identity: range2189293
display id: range2189293
version: 1
direction: direction/reverse
top level: BBa_K777003
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189297
display id: annotation2189297
version: 1
top level: BBa_K777003
location: https://synbiohub.org/public/igem/BBa_K777003/annotation2189297/range2189297/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777003/annotation2189297/range2189297/1
type: Range
persistent identity: range2189297
display id: range2189297
version: 1
direction: direction/reverse
top level: BBa_K777003
start: 42
end: 1703
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: later
mutable notes: N/A
mutable provenance: later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K777001
type: ComponentDefinition
persistent identity: BBa K777001
display id: BBa_K777001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23100
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777000
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K777001_sequence/1

BBa_K777000
type: Component
persistent identity: component2189126
display id: component2189126
version: 1
top level: BBa_K777001
access: public
definition: BBa_K777000

BBa_J23100
type: Component
persistent identity: component2189122
display id: component2189122
version: 1
top level: BBa_K777001
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189122
display id: annotation2189122
version: 1
top level: BBa_K777001
location: https://synbiohub.org/public/igem/BBa_K777001/annotation2189122/range2189122/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777001/annotation2189122/range2189122/1
type: Range
persistent identity: range2189122
display id: range2189122
version: 1
direction: direction/reverse
top level: BBa_K777001
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189126
display id: annotation2189126
version: 1
top level: BBa_K777001
location: https://synbiohub.org/public/igem/BBa_K777001/annotation2189126/range2189126/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777001/annotation2189126/range2189126/1
type: Range
persistent identity: range2189126
display id: range2189126
version: 1
direction: direction/reverse
top level: BBa_K777001
start: 42
end: 1703
orientation: inline

BBa_K777129
type: ComponentDefinition
persistent identity: BBa K777129
display id: BBa_K777129
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive J23109 promoter
creator: Team Goettingen
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23109 J23109]) and the plasmid backbone pSB1C3.
mutable notes: * No specific design considerations
mutable provenance: * pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: constitutive promoter J23109
sequence annotation: J23109
sequence: https://synbiohub.org/public/igem/BBa_K777129_sequence/1

constitutive promoter J23109
type: SequenceAnnotation
persistent identity: annotation2204889
display id: annotation2204889
version: 1
top level: BBa_K777129
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K777129/annotation2204889/range2204889/1

https://synbiohub.org/public/igem/BBa_K777129/annotation2204889/range2204889/1
type: Range
persistent identity: range2204889
display id: range2204889
version: 1
direction: direction/forward
top level: BBa_K777129
start: 2
end: 36
orientation: inline

J23109
type: SequenceAnnotation
persistent identity: annotation2204888
display id: annotation2204888
version: 1
top level: BBa_K777129
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777129/annotation2204888/range2204888/1

https://synbiohub.org/public/igem/BBa_K777129/annotation2204888/range2204888/1
type: Range
persistent identity: range2204888
display id: range2204888
version: 1
direction: direction/forward
top level: BBa_K777129
start: 2
end: 36
orientation: inline

BBa_K777128
type: ComponentDefinition
persistent identity: BBa K777128
display id: BBa_K777128
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive J23106 promoter
creator: Team Goettingen
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23106 J23106]) and the plasmid backbone pSB1C3.
mutable notes: * No specific design considerations
mutable provenance: * pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: J23106
sequence annotation: constitutive promoter J23106
sequence: https://synbiohub.org/public/igem/BBa_K777128_sequence/1

J23106
type: SequenceAnnotation
persistent identity: annotation2204868
display id: annotation2204868
version: 1
top level: BBa_K777128
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777128/annotation2204868/range2204868/1

https://synbiohub.org/public/igem/BBa_K777128/annotation2204868/range2204868/1
type: Range
persistent identity: range2204868
display id: range2204868
version: 1
direction: direction/forward
top level: BBa_K777128
start: 2
end: 36
orientation: inline

constitutive promoter J23106
type: SequenceAnnotation
persistent identity: annotation2204869
display id: annotation2204869
version: 1
top level: BBa_K777128
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K777128/annotation2204869/range2204869/1

https://synbiohub.org/public/igem/BBa_K777128/annotation2204869/range2204869/1
type: Range
persistent identity: range2204869
display id: range2204869
version: 1
direction: direction/forward
top level: BBa_K777128
start: 2
end: 36
orientation: inline

BBa_K777008
type: ComponentDefinition
persistent identity: BBa K777008
display id: BBa_K777008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23114
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23114 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23114 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23114
component: BBa_K777000
sequence annotation: BBa_K777000
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K777008_sequence/1

BBa_J23114
type: Component
persistent identity: component2189404
display id: component2189404
version: 1
top level: BBa_K777008
access: public
definition: BBa_J23114

BBa_K777000
type: Component
persistent identity: component2189408
display id: component2189408
version: 1
top level: BBa_K777008
access: public
definition: BBa_K777000

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189408
display id: annotation2189408
version: 1
top level: BBa_K777008
location: https://synbiohub.org/public/igem/BBa_K777008/annotation2189408/range2189408/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777008/annotation2189408/range2189408/1
type: Range
persistent identity: range2189408
display id: range2189408
version: 1
direction: direction/reverse
top level: BBa_K777008
start: 42
end: 1703
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2189404
display id: annotation2189404
version: 1
top level: BBa_K777008
location: https://synbiohub.org/public/igem/BBa_K777008/annotation2189404/range2189404/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777008/annotation2189404/range2189404/1
type: Range
persistent identity: range2189404
display id: range2189404
version: 1
direction: direction/reverse
top level: BBa_K777008
start: 1
end: 35
orientation: inline

BBa_J23111
type: ComponentDefinition
persistent identity: BBa J23111
display id: BBa_J23111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23111_sequence/1

BBa_K777101
type: ComponentDefinition
persistent identity: BBa K777101
display id: BBa_K777101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect motility in ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was from partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K777100
sequence: https://synbiohub.org/public/igem/BBa_K777101_sequence/1

BBa_K777100
type: Component
persistent identity: component2186537
display id: component2186537
version: 1
top level: BBa_K777101
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23100
type: Component
persistent identity: component2186529
display id: component2186529
version: 1
top level: BBa_K777101
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2186529
display id: annotation2186529
version: 1
top level: BBa_K777101
location: https://synbiohub.org/public/igem/BBa_K777101/annotation2186529/range2186529/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777101/annotation2186529/range2186529/1
type: Range
persistent identity: range2186529
display id: range2186529
version: 1
direction: direction/reverse
top level: BBa_K777101
start: 1
end: 35
orientation: inline

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2186537
display id: annotation2186537
version: 1
top level: BBa_K777101
location: https://synbiohub.org/public/igem/BBa_K777101/annotation2186537/range2186537/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777101/annotation2186537/range2186537/1
type: Range
persistent identity: range2186537
display id: range2186537
version: 1
direction: direction/reverse
top level: BBa_K777101
start: 42
end: 910
orientation: inline

BBa_K777006
type: ComponentDefinition
persistent identity: BBa K777006
display id: BBa_K777006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23112
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23112 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23112
component: BBa_K777000
sequence annotation: BBa_J23112
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K777006_sequence/1

BBa_J23112
type: Component
persistent identity: component2189394
display id: component2189394
version: 1
top level: BBa_K777006
access: public
definition: BBa_J23112

BBa_K777000
type: Component
persistent identity: component2189398
display id: component2189398
version: 1
top level: BBa_K777006
access: public
definition: BBa_K777000

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2189394
display id: annotation2189394
version: 1
top level: BBa_K777006
location: https://synbiohub.org/public/igem/BBa_K777006/annotation2189394/range2189394/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777006/annotation2189394/range2189394/1
type: Range
persistent identity: range2189394
display id: range2189394
version: 1
direction: direction/reverse
top level: BBa_K777006
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189398
display id: annotation2189398
version: 1
top level: BBa_K777006
location: https://synbiohub.org/public/igem/BBa_K777006/annotation2189398/range2189398/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777006/annotation2189398/range2189398/1
type: Range
persistent identity: range2189398
display id: range2189398
version: 1
direction: direction/reverse
top level: BBa_K777006
start: 42
end: 1703
orientation: inline

CP2LacPI1
type: ComponentDefinition
persistent identity: BBa K418003
display id: BBa_K418003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6938
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23104
component: BBa_Q01121
sequence annotation: BBa_Q01121
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K418003_sequence/1

BBa_J23104
type: Component
persistent identity: component2263763
display id: component2263763
version: 1
top level: CP2LacPI1
access: public
definition: BBa_J23104

BBa_Q01121
type: Component
persistent identity: component2263782
display id: component2263782
version: 1
top level: CP2LacPI1
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2263782
display id: annotation2263782
version: 1
top level: CP2LacPI1
location: https://synbiohub.org/public/igem/BBa_K418003/annotation2263782/range2263782/1
component: BBa_Q01121

https://synbiohub.org/public/igem/BBa_K418003/annotation2263782/range2263782/1
type: Range
persistent identity: range2263782
display id: range2263782
version: 1
direction: direction/reverse
top level: CP2LacPI1
start: 44
end: 1416
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2263763
display id: annotation2263763
version: 1
top level: CP2LacPI1
location: https://synbiohub.org/public/igem/BBa_K418003/annotation2263763/range2263763/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K418003/annotation2263763/range2263763/1
type: Range
persistent identity: range2263763
display id: range2263763
version: 1
direction: direction/reverse
top level: CP2LacPI1
start: 1
end: 35
orientation: inline

BBa_K823006
type: ComponentDefinition
persistent identity: BBa K823006
display id: BBa_K823006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23102
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:45:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23102 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: -35 box
sequence annotation: J23102
sequence: https://synbiohub.org/public/igem/BBa_K823006_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182514
display id: annotation2182514
version: 1
top level: BBa_K823006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1
type: Range
persistent identity: range2182514
display id: range2182514
version: 1
direction: direction/forward
top level: BBa_K823006
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182513
display id: annotation2182513
version: 1
top level: BBa_K823006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1
type: Range
persistent identity: range2182513
display id: range2182513
version: 1
direction: direction/forward
top level: BBa_K823006
start: 1
end: 6
orientation: inline

J23102
type: SequenceAnnotation
persistent identity: annotation2182512
display id: annotation2182512
version: 1
top level: BBa_K823006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1
type: Range
persistent identity: range2182512
display id: range2182512
version: 1
direction: direction/forward
top level: BBa_K823006
start: 1
end: 35
orientation: inline

BBa_K777110
type: ComponentDefinition
persistent identity: BBa K777110
display id: BBa_K777110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliC under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. coli''. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''fliC'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: * The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR. * ''fliC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatggcacaagtcattaatacc-3' ** Rev: 5'-gtttcttcgaattcgcggccgcttctagttaaccctgGagcagagacagaacc-3' (the capitalized "G" induces the mutation for removal of the third PstI site)
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K777109
sequence annotation: BBa_J23100
sequence annotation: BBa_K777109
sequence: https://synbiohub.org/public/igem/BBa_K777110_sequence/1

BBa_J23100
type: Component
persistent identity: component2189303
display id: component2189303
version: 1
top level: BBa_K777110
access: public
definition: BBa_J23100

BBa_K777109
type: Component
persistent identity: component2189311
display id: component2189311
version: 1
top level: BBa_K777110
access: public
definition: https://synbiohub.org/public/igem/BBa_K777109/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189303
display id: annotation2189303
version: 1
top level: BBa_K777110
location: https://synbiohub.org/public/igem/BBa_K777110/annotation2189303/range2189303/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777110/annotation2189303/range2189303/1
type: Range
persistent identity: range2189303
display id: range2189303
version: 1
direction: direction/reverse
top level: BBa_K777110
start: 1
end: 35
orientation: inline

BBa_K777109
type: SequenceAnnotation
persistent identity: annotation2189311
display id: annotation2189311
version: 1
top level: BBa_K777110
location: https://synbiohub.org/public/igem/BBa_K777110/annotation2189311/range2189311/1
component: BBa_K777109

https://synbiohub.org/public/igem/BBa_K777110/annotation2189311/range2189311/1
type: Range
persistent identity: range2189311
display id: range2189311
version: 1
direction: direction/reverse
top level: BBa_K777110
start: 42
end: 1538
orientation: inline