Promoter Family Member (U037O11+D038O22)
Promoter Family Member (U073O11+D002O22)
NOT Gate Promoter Family Member (D001O44)
NOT Gate Promoter Family Member (D001O22)
Promoter Family Member (U085O11+D050O22)
Promoter Family Member (U097NUL+D062O22)
Promoter Family Member (U073O27+D062NUL)
Promoter Family Member (U097O11+D038O22)
Promoter Family Member with Hybrid Operator (D001O12)
Promoter Family Member with Hybrid Operator (D001O17)
Promoter Family Member with Hybrid Operator (D001O26)
Promoter Family Member with Hybrid Operator (D001O27)
Promoter Family Member (U049O11+D014O22)
Promoter Family Member (U073O26+D062O61)
Promoter Family Member with Hybrid Operator (D001O61)
Promoter Family Member (U073O11+D014O22)
Promoter Family Member (U061O11+D038O22)
Promoter Family Member (U049O11+D038O22)
Promoter Family Member (U049O11+D062O22)
Promoter Family Member (U085O11+D062NUL)
Promoter Family Member with Hybrid Operator (D001O24)
Promoter Family Member (U085O11+D062O22)
Promoter Family Member (U037O11+D062NUL)
A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732403.
Promoter Family Member (U037O11+D062O22)
Promoter Family Member (U097O11+D002O22)
Promoter Family Member with Hybrid Operator (D001O21)
Promoter Family Member (U097O11+D062O22)
Promoter Family Member (U073O11+D062O22)
Promoter Family Member (U049O11+D002O22)
Promoter Family Member (U073O11+D050O22)
Promoter Family Member (U061O11+D026O22)
Promoter Family Member with Hybrid Operator (D001O47)
Promoter Family Member (U097NUL+D002O22)
A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3C5. For more information please refer to BBa_I732401.
Promoter Family Member (U073O11+D062NUL)
Promoter Family Member (U073O11+D026O22)
NOT Gate Promoter Family Member (D001O66)
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NOT Gate Promoter Family Member (D001O1wt1)
NOT Gate Promoter Family Member (D001O33)
Promoter Family Member (U097O11+D014O22)
Promoter Family Member (U097O11+D050O22)
Promoter Family Member (U097NUL+D014O22)
A Double Reporter (LacZ-alpha and RFP) loaded on pSB3C5. For more information please refer to BBa_I732402.
Promoter Family Member (U061O11+D062NUL)
A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732401.
Promoter Family Member (U037O11+D050O22)
Promoter Family Member (U085O11+D038O22)
Promoter Family Member with Hybrid Operator (D001O16)
Promoter Family Member (U073O26+D062NUL)
Promoter Family Member (U061O11+D014O22)
NOT Gate Promoter Family Member (D001O77)
Promoter Family Member (U085O11+D026O22)
Promoter Family Member (U061O11+D050O22)
Promoter Family Member (U097O11+D062NUL)
Promoter Family Member (U073O11+D038O22)
NOT Gate Promoter Family Member (D001O55)
Promoter Family Member (U097NUL+D050O22)
Promoter Family Member (U049O11+D062NUL)
A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732402.
Promoter Family Member (U037O11+D002O22)
Promoter Family Member (U049O11+D026O22)
Promoter Family Member (U037O11+D026O22)
Promoter Family Member (U097NUL+D026O22)
A Double Reporter (LacZ-alpha and GFP) loaded on pSB3C5. For more information please refer to BBa_I732403.
Promoter Family Member (U049O11+D050O22)
Promoter Family Member (U061O11+D002O22)
Promoter Family Member (U097NUL+D062NUL)
P_template1 serves as the truss for PCR cloning. By respectively applying 7 different primers upstream and downstream, that is, 49 pairs of primers in total, we have got 49 different promoters. Then we made them digsted and loaded into a vector that has already possessed a lacZ-alpha fragment. In this way, we can read from the color of the clones on the plate whether the inserted promoter works or not. By this means, We can try seven different positions for Operator A and seven for Operator B, thus constructing 7*7 kinds of promoters which have actually constituted a specific promoter family. After transformed into the four test plasmids (pCR00, pCR01, pCR10, pCR11) together with a downstream double-reporter system, we will get to know the solo-repression and co-repression effects of the two repressors on the specific promoter.
Promoter Family Member (U097O11+D026O22)
Promoter Family Member (U061O11+D062O22)
Promoter Family Member (U085O11+D002O22)
NOT Gate Promoter Family Member (D001O11)
Promoter Family Member (U037O11+D014O22)
Promoter Family Member (U097NUL+D038O22)
Promoter Family Member with Hybrid Operator (D001O46)
Promoter Family Member (U085O11+D014O22)