//promoter/stresskit
type: Collection
persistent identity: promoter stresskit
display id: promoter_stresskit
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K086028
member: BBa_K086023
member: BBa_K086026
member: BBa_K086025
member: BBa_K086032
member: BBa_K086017
member: BBa_K086021
member: BBa_K086019
member: pUSPA
member: BBa_K283031
member: BBa_K086030
member: BBa_K086022
member: BBa_K086024
member: BBa_K086029
member: BBa_K086027
member: BBa_K086018
member: BBa_K086020
member: BBa_K086031
member: BBa_K086033

BBa_K086029
type: ComponentDefinition
persistent identity: BBa K086029
display id: BBa_K086029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_4
sequence: https://synbiohub.org/public/igem/BBa_K086029_sequence/1

P32_4
type: SequenceAnnotation
persistent identity: annotation1995763
display id: annotation1995763
version: 1
top level: BBa_K086029
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086029/annotation1995763/range1995763/1

https://synbiohub.org/public/igem/BBa_K086029/annotation1995763/range1995763/1
type: Range
persistent identity: range1995763
display id: range1995763
version: 1
direction: direction/forward
top level: BBa_K086029
start: 1
end: 55
orientation: inline

BBa_K086032
type: ComponentDefinition
persistent identity: BBa K086032
display id: BBa_K086032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: cleaned
mutable provenance: Lac O and Sigma 38 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_3
sequence: https://synbiohub.org/public/igem/BBa_K086032_sequence/1

P38_3
type: SequenceAnnotation
persistent identity: annotation1995774
display id: annotation1995774
version: 1
top level: BBa_K086032
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086032/annotation1995774/range1995774/1

https://synbiohub.org/public/igem/BBa_K086032/annotation1995774/range1995774/1
type: Range
persistent identity: range1995774
display id: range1995774
version: 1
direction: direction/forward
top level: BBa_K086032
start: 1
end: 55
orientation: inline

BBa_K086021
type: ComponentDefinition
persistent identity: BBa K086021
display id: BBa_K086021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: Lac O and sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_4
sequence: https://synbiohub.org/public/igem/BBa_K086021_sequence/1

P24_4
type: SequenceAnnotation
persistent identity: annotation1995755
display id: annotation1995755
version: 1
top level: BBa_K086021
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086021/annotation1995755/range1995755/1

https://synbiohub.org/public/igem/BBa_K086021/annotation1995755/range1995755/1
type: Range
persistent identity: range1995755
display id: range1995755
version: 1
direction: direction/forward
top level: BBa_K086021
start: 1
end: 55
orientation: inline

BBa_K086018
type: ComponentDefinition
persistent identity: BBa K086018
display id: BBa_K086018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: LacO and Sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_1
sequence: https://synbiohub.org/public/igem/BBa_K086018_sequence/1

P24_1
type: SequenceAnnotation
persistent identity: annotation1995752
display id: annotation1995752
version: 1
top level: BBa_K086018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086018/annotation1995752/range1995752/1

https://synbiohub.org/public/igem/BBa_K086018/annotation1995752/range1995752/1
type: Range
persistent identity: range1995752
display id: range1995752
version: 1
direction: direction/forward
top level: BBa_K086018
start: 1
end: 55
orientation: inline

BBa_K086020
type: ComponentDefinition
persistent identity: BBa K086020
display id: BBa_K086020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: Lac O and sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_3
sequence: https://synbiohub.org/public/igem/BBa_K086020_sequence/1

P24_3
type: SequenceAnnotation
persistent identity: annotation1995754
display id: annotation1995754
version: 1
top level: BBa_K086020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086020/annotation1995754/range1995754/1

https://synbiohub.org/public/igem/BBa_K086020/annotation1995754/range1995754/1
type: Range
persistent identity: range1995754
display id: range1995754
version: 1
direction: direction/forward
top level: BBa_K086020
start: 1
end: 55
orientation: inline

BBa_K086033
type: ComponentDefinition
persistent identity: BBa K086033
display id: BBa_K086033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: cleaned
mutable provenance: Lac O and sigma 38 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_4
sequence: https://synbiohub.org/public/igem/BBa_K086033_sequence/1

P38_4
type: SequenceAnnotation
persistent identity: annotation1995775
display id: annotation1995775
version: 1
top level: BBa_K086033
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086033/annotation1995775/range1995775/1

https://synbiohub.org/public/igem/BBa_K086033/annotation1995775/range1995775/1
type: Range
persistent identity: range1995775
display id: range1995775
version: 1
direction: direction/forward
top level: BBa_K086033
start: 1
end: 55
orientation: inline

BBa_K086031
type: ComponentDefinition
persistent identity: BBa K086031
display id: BBa_K086031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: Cleaned
mutable provenance: Lac O and sigma 38 binding sites conserved sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_2
sequence: https://synbiohub.org/public/igem/BBa_K086031_sequence/1

P38_2
type: SequenceAnnotation
persistent identity: annotation1995765
display id: annotation1995765
version: 1
top level: BBa_K086031
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086031/annotation1995765/range1995765/1

https://synbiohub.org/public/igem/BBa_K086031/annotation1995765/range1995765/1
type: Range
persistent identity: range1995765
display id: range1995765
version: 1
direction: direction/forward
top level: BBa_K086031
start: 1
end: 55
orientation: inline

BBa_K086025
type: ComponentDefinition
persistent identity: BBa K086025
display id: BBa_K086025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: cleaned
mutable provenance: Lac O and sigma 28 binding sites' conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P28_4
sequence: https://synbiohub.org/public/igem/BBa_K086025_sequence/1

P28_4
type: SequenceAnnotation
persistent identity: annotation1995759
display id: annotation1995759
version: 1
top level: BBa_K086025
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086025/annotation1995759/range1995759/1

https://synbiohub.org/public/igem/BBa_K086025/annotation1995759/range1995759/1
type: Range
persistent identity: range1995759
display id: range1995759
version: 1
direction: direction/forward
top level: BBa_K086025
start: 1
end: 55
orientation: inline

BBa_K086023
type: ComponentDefinition
persistent identity: BBa K086023
display id: BBa_K086023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: cleaned
mutable provenance: Lac O and Sigma 28 binding sites' conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P28_2
sequence: https://synbiohub.org/public/igem/BBa_K086023_sequence/1

P28_2
type: SequenceAnnotation
persistent identity: annotation1995757
display id: annotation1995757
version: 1
top level: BBa_K086023
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086023/annotation1995757/range1995757/1

https://synbiohub.org/public/igem/BBa_K086023/annotation1995757/range1995757/1
type: Range
persistent identity: range1995757
display id: range1995757
version: 1
direction: direction/forward
top level: BBa_K086023
start: 1
end: 55
orientation: inline

BBa_K086027
type: ComponentDefinition
persistent identity: BBa K086027
display id: BBa_K086027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_2
sequence: https://synbiohub.org/public/igem/BBa_K086027_sequence/1

P32_2
type: SequenceAnnotation
persistent identity: annotation1995761
display id: annotation1995761
version: 1
top level: BBa_K086027
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086027/annotation1995761/range1995761/1

https://synbiohub.org/public/igem/BBa_K086027/annotation1995761/range1995761/1
type: Range
persistent identity: range1995761
display id: range1995761
version: 1
direction: direction/forward
top level: BBa_K086027
start: 1
end: 55
orientation: inline

BBa_K086022
type: ComponentDefinition
persistent identity: BBa K086022
display id: BBa_K086022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: Cleaned
mutable provenance: Lac and sigma 28 conserved binding sites
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P28_1
sequence: https://synbiohub.org/public/igem/BBa_K086022_sequence/1

P28_1
type: SequenceAnnotation
persistent identity: annotation1995756
display id: annotation1995756
version: 1
top level: BBa_K086022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086022/annotation1995756/range1995756/1

https://synbiohub.org/public/igem/BBa_K086022/annotation1995756/range1995756/1
type: Range
persistent identity: range1995756
display id: range1995756
version: 1
direction: direction/forward
top level: BBa_K086022
start: 1
end: 55
orientation: inline

BBa_K283031
type: ComponentDefinition
persistent identity: BBa K283031
display id: BBa_K283031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FF_FF ansB promoter (E.coli)
creator: Shi Lei
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: inducible hypoxia promoter
mutable notes: BBa_283030
mutable provenance: BBa_283030
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FNR
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K283031_sequence/1

FNR
type: SequenceAnnotation
persistent identity: annotation2056553
display id: annotation2056553
version: 1
top level: BBa_K283031
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K283031/annotation2056553/range2056553/1

https://synbiohub.org/public/igem/BBa_K283031/annotation2056553/range2056553/1
type: Range
persistent identity: range2056553
display id: range2056553
version: 1
direction: direction/forward
top level: BBa_K283031
start: 53
end: 74
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2056551
display id: annotation2056551
version: 1
top level: BBa_K283031
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K283031/annotation2056551/range2056551/1

https://synbiohub.org/public/igem/BBa_K283031/annotation2056551/range2056551/1
type: Range
persistent identity: range2056551
display id: range2056551
version: 1
direction: direction/forward
top level: BBa_K283031
start: 1
end: 105
orientation: inline

FNR
type: SequenceAnnotation
persistent identity: annotation2056552
display id: annotation2056552
version: 1
top level: BBa_K283031
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K283031/annotation2056552/range2056552/1

https://synbiohub.org/public/igem/BBa_K283031/annotation2056552/range2056552/1
type: Range
persistent identity: range2056552
display id: range2056552
version: 1
direction: direction/forward
top level: BBa_K283031
start: 1
end: 24
orientation: inline

BBa_K086019
type: ComponentDefinition
persistent identity: BBa K086019
display id: BBa_K086019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24
mutable notes: Cleaned
mutable provenance: Lac O and sigma 24 binding conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_2
sequence: https://synbiohub.org/public/igem/BBa_K086019_sequence/1

P24_2
type: SequenceAnnotation
persistent identity: annotation1995753
display id: annotation1995753
version: 1
top level: BBa_K086019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086019/annotation1995753/range1995753/1

https://synbiohub.org/public/igem/BBa_K086019/annotation1995753/range1995753/1
type: Range
persistent identity: range1995753
display id: range1995753
version: 1
direction: direction/forward
top level: BBa_K086019
start: 1
end: 55
orientation: inline

BBa_K086028
type: ComponentDefinition
persistent identity: BBa K086028
display id: BBa_K086028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites' promoter sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_3
sequence: https://synbiohub.org/public/igem/BBa_K086028_sequence/1

P32_3
type: SequenceAnnotation
persistent identity: annotation1995762
display id: annotation1995762
version: 1
top level: BBa_K086028
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086028/annotation1995762/range1995762/1

https://synbiohub.org/public/igem/BBa_K086028/annotation1995762/range1995762/1
type: Range
persistent identity: range1995762
display id: range1995762
version: 1
direction: direction/forward
top level: BBa_K086028
start: 1
end: 55
orientation: inline

pUSPA
type: ComponentDefinition
persistent identity: BBa K1620000
display id: BBa_K1620000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter element UspA
creator: Celio Dias Santos Jr
created: 2015-09-02T11:00:00Z
modified: 2015-09-05T12:59:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2037_
m user id: 23597
owner id: 23597
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The universal stress protein A is a response of Escherichia coli cells to growth arrest, and its lacking generates cells with defective growth.This genetic element is dependent to sigma-70 factor. Its regulation is done through the concentration of a specific stationary phase allormone, guanosine-5'-diphosphate-3'-diphosphate (ppGp), as described elsewhere. The ppGp activate the transcription of downstream elements through a positive regulation of the β-subunit of RNA polymerase. In this sense, the PuspA element is considered a stationary phase promoter. However, its behavior is like a constitutive transcription promotion, becoming higher in stress situations. Diverse conditions make the Escherichia coli cell enter to stress state, like heat shocks, starvation, osmotic stress, ultraviolet light and other conditions. In these situations, the RNA polimerase sigma factors (ropS) trigger the expression of chaperones and other stress protector molecules, in order to help the cell survive. In our project we have used this promoter to trigger the expression of limonene synthase after an osmotic shock. Other uses of this part includes the environmental stress detectors making using a reporter system coupled to this promoter.
mutable notes: The original promoter site was shortened and the last three nucleotides were changed to restriction site adjustment.
mutable provenance: We have drawn the sequence for an improved PuspA promoter from an analysis of 400bp upstream the Universal Protein A (UspA) gene in E. coli K12 MG16555 genome. After design, the DNA template of part was synthesized by IDT and was amplified from a G-block and assembled using 3A assembly method.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: UspA
sequence: https://synbiohub.org/public/igem/BBa_K1620000_sequence/1

UspA
type: SequenceAnnotation
persistent identity: annotation2443284
display id: annotation2443284
version: 1
top level: pUSPA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1620000/annotation2443284/range2443284/1

https://synbiohub.org/public/igem/BBa_K1620000/annotation2443284/range2443284/1
type: Range
persistent identity: range2443284
display id: range2443284
version: 1
direction: direction/forward
top level: pUSPA
start: 1
end: 46
orientation: inline

BBa_K086017
type: ComponentDefinition
persistent identity: BBa K086017
display id: BBa_K086017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: unmodified Lutz-Bujard LacO promoter
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: unmodified Lutz-Bujard LacO promoter.
mutable notes: Cleaned
mutable provenance: Lac O
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K086017_sequence/1

LacO
type: SequenceAnnotation
persistent identity: annotation1995751
display id: annotation1995751
version: 1
top level: BBa_K086017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086017/annotation1995751/range1995751/1

https://synbiohub.org/public/igem/BBa_K086017/annotation1995751/range1995751/1
type: Range
persistent identity: range1995751
display id: range1995751
version: 1
direction: direction/forward
top level: BBa_K086017
start: 1
end: 55
orientation: inline

BBa_K086030
type: ComponentDefinition
persistent identity: BBa K086030
display id: BBa_K086030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38
mutable notes: cleaned
mutable provenance: Lac O and sigma 38 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P38_1
sequence: https://synbiohub.org/public/igem/BBa_K086030_sequence/1

P38_1
type: SequenceAnnotation
persistent identity: annotation1995764
display id: annotation1995764
version: 1
top level: BBa_K086030
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086030/annotation1995764/range1995764/1

https://synbiohub.org/public/igem/BBa_K086030/annotation1995764/range1995764/1
type: Range
persistent identity: range1995764
display id: range1995764
version: 1
direction: direction/forward
top level: BBa_K086030
start: 1
end: 55
orientation: inline

BBa_K086024
type: ComponentDefinition
persistent identity: BBa K086024
display id: BBa_K086024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28
mutable notes: cleaned
mutable provenance: Lac O and sigma 28 binding sites' conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P24_3
sequence: https://synbiohub.org/public/igem/BBa_K086024_sequence/1

P24_3
type: SequenceAnnotation
persistent identity: annotation1995758
display id: annotation1995758
version: 1
top level: BBa_K086024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086024/annotation1995758/range1995758/1

https://synbiohub.org/public/igem/BBa_K086024/annotation1995758/range1995758/1
type: Range
persistent identity: range1995758
display id: range1995758
version: 1
direction: direction/forward
top level: BBa_K086024
start: 1
end: 55
orientation: inline

BBa_K086026
type: ComponentDefinition
persistent identity: BBa K086026
display id: BBa_K086026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: modified Lutz-Bujard LacO promoter,with alternative sigma factor σ32
mutable notes: cleaned
mutable provenance: Lac O and sigma 32 binding sites conserved sequences
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P32_1
sequence: https://synbiohub.org/public/igem/BBa_K086026_sequence/1

P32_1
type: SequenceAnnotation
persistent identity: annotation1995760
display id: annotation1995760
version: 1
top level: BBa_K086026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086026/annotation1995760/range1995760/1

https://synbiohub.org/public/igem/BBa_K086026/annotation1995760/range1995760/1
type: Range
persistent identity: range1995760
display id: range1995760
version: 1
direction: direction/forward
top level: BBa_K086026
start: 1
end: 55
orientation: inline